12 results about "Glucose dehydrogenase" patented technology

The invention belongs to the field of metabolic engineering, and discloses a genetically engineered bacterium for producing myo-inositol as well as a construction method and application of the genetically engineered bacterium. The genetically engineered bacterium takes escherichia coli as an original strain, and is obtained by performing the following gene editing on a genome of the escherichia coli: non-expressed lactose operon repressor protein, glucose phosphate dehydrogenase, acetokinase, glucose phosphate isomerase and glycerol repressor protein; and overexpressing inositol-1-phosphate synthase, inositol monophosphate, glucokinase, glucose permease and glycerol kinase. Compared with the prior art, the genetically engineered bacterium disclosed by the invention has remarkable advantages in the aspects of key enzyme expression rate, metabolism specificity, fermentation period and the like, and an efficient, stable, economical and feasible solution is provided for industrial production of myo-inositol.

This application relates to a phenytoin detection kit. Specifically, the glucose-6-phosphate dehydrogenase mutant of this application contains one mutation selected from the following compared to the wild-type glucose-6-phosphate dehydrogenase: D306C, D375C, or G426C. The detection kit prepared using the glucose-6-phosphate dehydrogenase mutant of this application exhibits high specificity, high sensitivity, ease of operation, short detection time, and accurate quantification, making it suitable for high-throughput detection.

The present application relates to a preparation method of a conjugate. Specifically, the 6-phosphogluconate dehydrogenase mutant of the present application comprises one mutation or a combination thereof selected from D306C, D375C, G426C compared with wild-type 6-phosphogluconate dehydrogenase. The detection kit prepared by using the 6-phosphogluconate dehydrogenase mutant of the present application has high specificity, high sensitivity, convenient operation, short detection time, accurate quantification, and is suitable for high-throughput detection.

The present application relates to a preparation method of a conjugate. Specifically, the 6-phosphogluconate dehydrogenase mutant of the present application comprises one mutation or a combination thereof selected from D306C, D375C, G426C compared with wild-type 6-phosphogluconate dehydrogenase. The detection kit prepared by using the 6-phosphogluconate dehydrogenase mutant of the present application has high specificity, high sensitivity, convenient operation, short detection time, accurate quantification, and is suitable for high-throughput detection.

The invention belongs to the technical field of biochemical energy batteries, and discloses an implantable biochemical energy battery array, a chip and application. Comprising a plurality of biochemical energy power generation units which are mutually connected through a microfluidic channel to form a biochemical energy power generation array, the power generation array is connected in series and in parallel to form a battery array, and glucose fluid flows through the microfluidic channel. The power generation unit comprises a positive electrode, a biochemical decomposition chamber and a negative electrode, carrier materials of hexokinase HK, glucose 6-phosphate dehydrogenase G6PD, glucose 6-phosphate lactonase PGLS and glucose 6-phosphate dehydrogenase 6PGDH are fixed in the biochemical decomposition chamber, and the negative electrode is coated with an artificial film assembled with NADPH transhydrogenase and mitochondrial respiratory chain compound protein. According to the invention, glucose is used as fuel of the cell for continuous power generation, and the flexible array biochemical energy cell which is miniaturized, high in load, long in endurance, capable of being shaped and high in energy density can be provided.

This application relates to the use of conjugates in the preparation of diagnostic reagents. Specifically, the glucose-6-phosphate dehydrogenase mutant of this application contains one or a combination of mutations selected from the following, compared to wild-type glucose-6-phosphate dehydrogenase: D306C, D375C, and G426C. Diagnostic kits prepared using the glucose-6-phosphate dehydrogenase mutant of this application exhibit high specificity, high sensitivity, ease of operation, short detection time, and accurate quantification, making them suitable for high-throughput detection.

The application discloses high-yield gernien A engineering methanotrophs, a construction method and application thereof, and belongs to the technical field of metabolic engineering and synthetic biology. P mxaF expressing a gernien A synthase encoding gene gas overexpressing a 1-deoxy-xylose-5-phosphate synthase encoding gene in a gernien A synthesis pathway dxs1 and dxs2 a farnesyl pyrophosphate synthase encoding gene ispA a isopentenyl pyrophosphate isomerase encoding gene Bsidi simultaneously overexpressing 6-phosphogluconate dehydrogenase zwf1 and pfk1 and fba2 in an EMP pathway, to obtain gernien A engineering methanotrophs. The fermentation production of gernien A is carried out by using the engineering methanotrophs, and the yield of gernien A is obviously improved, so that the engineering methanotrophs have an industrial application prospect.

This invention discloses an engineered methanogenic bacterium that produces high levels of squalene, its construction method, and its applications, belonging to the fields of metabolic engineering and synthetic biology. This is achieved by using a strong promoter in the host bacterium (methanogenic bacterium). P mxaF Expression of squalene synthase encoding gene sqs Overexpression of the gene encoding 1-deoxy-xylulose-5-phosphate synthase in the squalene synthesis pathway dxs1 and dxs2 Farnesyl pyrophosphate synthase encoding gene ispA Isopentenyl pyrophosphate isomerase encoding gene My friends Simultaneously overexpressing glucose-6-phosphate dehydrogenase zwf1 and the EMP pathway pfk1 and fba2 An engineered methanogenic bacterium producing squalene was obtained. Using this engineered methanogenic bacterium for squalene fermentation production can significantly increase squalene yield and has excellent prospects for industrial application.

This application relates to a tacrolimus detection kit. Specifically, the glucose-6-phosphate dehydrogenase mutant of this application contains one or a combination of mutations selected from the following, compared to the wild-type glucose-6-phosphate dehydrogenase: D306C, D375C, and G426C. The detection kit prepared using the glucose-6-phosphate dehydrogenase mutant of this application exhibits high specificity, high sensitivity, ease of operation, short detection time, and accurate quantification, making it suitable for high-throughput detection.

The invention discloses a multi-channel double-layer membrane blood cell interference resisting electrochemical test piece and application thereof, and belongs to the technical field of electrochemical test pieces, the multi-channel double-layer membrane blood cell interference resisting electrochemical test piece comprises a base material, a sensing electrode, a top sealing layer and a double-layer membrane structure composed of an enzyme reaction membrane layer and a blood cell filtering membrane layer, and the double-layer membrane structure is located above the sensing electrode. According to the invention, a completely independent multi-channel microfluidic system is realized through physical structure partition, and a three-dimensional bulge structure is arranged at a blood inlet of each microfluidic channel, so that the blood suction starting speed and the sample entering efficiency are remarkably improved, and the problems of unstable blood suction efficiency and difficulty in avoiding cross interference of the existing multi-channel design are solved on the structural aspect. According to the invention, a double-layer membrane structure is adopted, a physical filter membrane layer with a stable aperture structure is constructed by blending PU / PVA, and the physical filter membrane layer is superposed on a glucose dehydrogenase reaction layer, so that red blood cells are prevented from entering a detection area from the source, and the influence of HCT on signals is reduced.

The application discloses a multi-channel double-layer membrane anti-blood cell interference electrochemical test sheet and application thereof, and belongs to the technical field of electrochemical test sheets, and comprises a base material, a sensing electrode, a top sealing layer, and a double-layer membrane structure composed of an enzyme reaction membrane layer and a blood cell filtration membrane layer, wherein the double-layer membrane structure is located above the sensing electrode. The application realizes a completely independent multi-channel microfluidic system through physical structure separation, and sets a three-dimensional protruding structure at the blood inlet of each microfluidic channel, thereby significantly improving blood absorption starting speed and sample entering efficiency, and solving the problems of unstable blood absorption efficiency and difficult-to-avoid cross interference in the existing multi-channel design in the structural aspect. The application adopts a double-layer membrane structure, constructs a physical filter membrane layer with a stable pore size structure through PU / PVA blending, and stacks the physical filter membrane layer on a glucose dehydrogenase reaction layer, so that red blood cells are blocked from entering a detection area from the source, and the influence of HCT on a signal is reduced.

The present application relates to a cortisol detection kit. Specifically, the 6-phosphogluconate dehydrogenase mutant of the present application comprises one mutation or a combination thereof selected from the following: D306C, D375C, G426C, compared with wild-type 6-phosphogluconate dehydrogenase. The detection kit prepared using the 6-phosphogluconate dehydrogenase mutant of the present application has strong specificity, high sensitivity, convenient operation, short detection time, accurate quantification, and is suitable for high-throughput detection.