3 results about "Glucose dehydrogenase" patented technology

This invention discloses an engineered methanogenic bacterium that produces high levels of squalene, its construction method, and its applications, belonging to the fields of metabolic engineering and synthetic biology. This is achieved by using a strong promoter in the host bacterium (methanogenic bacterium). P mxaF Expression of squalene synthase encoding gene sqs Overexpression of the gene encoding 1-deoxy-xylulose-5-phosphate synthase in the squalene synthesis pathway dxs1 and dxs2 Farnesyl pyrophosphate synthase encoding gene ispA Isopentenyl pyrophosphate isomerase encoding gene My friends Simultaneously overexpressing glucose-6-phosphate dehydrogenase zwf1 and the EMP pathway pfk1 and fba2 An engineered methanogenic bacterium producing squalene was obtained. Using this engineered methanogenic bacterium for squalene fermentation production can significantly increase squalene yield and has excellent prospects for industrial application.

The application discloses a multi-channel double-layer membrane anti-blood cell interference electrochemical test sheet and application thereof, and belongs to the technical field of electrochemical test sheets, and comprises a base material, a sensing electrode, a top sealing layer, and a double-layer membrane structure composed of an enzyme reaction membrane layer and a blood cell filtration membrane layer, wherein the double-layer membrane structure is located above the sensing electrode. The application realizes a completely independent multi-channel microfluidic system through physical structure separation, and sets a three-dimensional protruding structure at the blood inlet of each microfluidic channel, thereby significantly improving blood absorption starting speed and sample entering efficiency, and solving the problems of unstable blood absorption efficiency and difficult-to-avoid cross interference in the existing multi-channel design in the structural aspect. The application adopts a double-layer membrane structure, constructs a physical filter membrane layer with a stable pore size structure through PU / PVA blending, and stacks the physical filter membrane layer on a glucose dehydrogenase reaction layer, so that red blood cells are blocked from entering a detection area from the source, and the influence of HCT on a signal is reduced.