68results about How to "Increase dehydrogenase activity" patented technology

The present invention is directed to glucose dehydrogenase (GDH) polypeptides that have enhanced GDH activity and / or thermostability relative to the backbone wild-type glucose dehydrogenase polypeptide. In addition, the present invention is directed to a polynucleotide that encodes for the GDH polypeptides of the present invention, to nucleic acid sequences comprising the polynucleotides, to expression vectors comprising the polynucleotides operatively linked to a promoter, to host cells transformed to express the GDH polypeptides, and to a method for producing the GDH polypeptides of the present invention.

The invention relates to the field of microorganisms and discloses a microbial strain capable of remedying heavy metal-contaminated soil and application of the microbial strain. The preservation number of the microbial strain is CGMCC No.10843. A strain of burkholderia cepacia is screened from the soil contaminated by heavy metal cadmium and can purify the exchangeable cadmium in the soil and significantly reduce the content of the exchangeable cadmium. The microbial strain is extremely-strong in tolerance to the cadmium and small in soil disturbance, has a stronger biological remediation function, meanwhile can improve the activity of soil dehydrogenase and can also be applied to the remediation of the heavy metal-contaminated soil and the improvement of the activity of the soil dehydrogenase as well as the preparation of relevant products.

The invention discloses red mud-assisted soil remediation fertilizer. The red mud-assisted soil remediation fertilizer is prepared from, by weight, 24-26 parts of wheat gluten flour, 8-10 parts of humus soil, 28-32 parts of cottonseed cakes, 68-74 parts of red mud, 2-3 parts of potassium selenite, 8-10 parts of humic acidic ammonium, 40-45 parts of kapok hulls, 13-15 parts of siderite slag, 13-15 parts of limonite slag, 40-45 parts of orange peel, 4-5 parts of an EM compound microorganism bacterium agent and an appropriate amount of water. According to the soil remediation fertilizer, the red mud materials are utilized to be combined with the humic raw materials, the content of soil nutrients, especially silicon, can be increased, the content of biological effective-state heavy metal is simultaneously decreased, the stabilization effect is improved, accumulation of the heavy metal in organisms is reduced, and the purpose of soil improvement is achieved to enable the soil to be beneficial for crop growth.

The invention discloses organic polluted soil remediation fertilizer which is prepared from, by weight, 10-12 parts of shell powder, 23-25 parts of bone meal, 5-6 parts of polyaspartic acid, 14-16 parts of powdered straw sugar, 1-3 parts of potassium chlorate, 2-3 parts of compound sodium nitrophenolate, 34-36 parts of eucalyptus saw powder, 13-14 parts of siderite slag, 13-14 parts of limonite slag, 55-60 parts of orange peel, 5-6 parts of EM compound microorganism bacterium agents, and an appropriate amount of water. The soil remediation fertilizer is applicable to deodorization and soil regeneration for soil polluted by waste in a brewery, a slaughter factory, a livestock farm and the like, water and oil in the pollutant are adsorbed and gathered through organic strong adsorption materials such as the shell powder and the bone meal, and microbial decomposition is carried out in an auxiliary mode, so that the ecological environment of the polluted soil is greatly improved, pathogenic bacteria breeding is prevented, the improved soil can be used as a seedling culture substrate, and the utilization value of the improved soil is improved.

The present invention is directed to glucose dehydrogenase (GDH) polypeptides that have enhanced GDH activity and / or thermostability relative to the backbone wild-type glucose dehydrogenase polypeptide. In addition, the present invention is directed to a polynucleotide that encodes for the GDH polypeptides of the present invention, to nucleic acid sequences comprising the polynucleotides, to expression vectors comprising the polynucleotides operatively linked to a promoter, to host cells transformed to express the GDH polypeptides, and to a method for producing the GDH polypeptides of the present invention.

The invention relates to a complex biological growth promoter used for recovering river bottom sediment, which comprises the following components (by weight percent: 5 to 14 % of electrical stone, 85 to 94% of active zeolite, 0.3 to 1% of biochemical fulvic acids and 0.1 to 0.3% of CoCl2.6H2O, wherein natural electrical stone is used as the raw material of the electrical stone, and the natural electrical stone is crushed into super fine powder with the granularity of 800 to 1200 meshes; natural active zeolite is used as the raw material of the active zeolite, the natural active zeolite is firstly activated at the high temperature of 300 to 600 DEG C and then crushed into active zeolite powder with the granularity of 100 to 500 meshes; and the complex biological growth promoter used for recovering river bottom sediment can be obtained after the electrical stone, the active zeolite, the biochemical fulvic acids and the CoCl2.6H2O are dried, crushed and mixed. The invention has the characteristics of activity preservation, simple production process and low production cost and suitability for large-size application, and is not easy to lose along with water.

The invention discloses a soil restoration fertilizer with the passivated heavy metal mobility. The soil restoration fertilizer with the passivated heavy metal mobility is prepared from, by weight, 20-24 parts of paulownia sawdust, 34-36 parts of Chinese medicine residues, 4-5 parts of gelatin, 16-18 parts of kaolin, 1-2 parts of sodium polyphosphate, 1-2 parts of polymeric aluminum chloride, 1-3 parts of zinc chloride, 48-50 parts of eucalyptus sawdust, 12-14 parts of siderite slag, 12-14 parts of limonite slag, 45-55 parts of orange peel, 3-4 parts of an EM composite microbial agent and a proper amount of water. The soil restoration fertilizer is modified through mixed calcination of kaolin and sodium polyphosphate, the buffering capacity of soil in use is improved, the adsorption capacity of soil for heavy metal is enhanced, migration of heavy metal in soil to plants is inhibited, eating safety of crop products is guaranteed, meanwhile, the conditions of leaching, infiltration and the like of heavy metal can be reduced, underground water resources are prevented from being polluted, and health of a soil ecological environment is guaranteed.

The invention belongs to the field of alcoholism relieving beverage, in particular to Chinese medicinal alcoholism relieving health-care beverage with high safety, high drug effect, stable and consistent quality control and acute and chronic alcoholism resistance, and a preparation method thereof. The beverage is prepared from the following raw materials in part by weight: 0.1 to 2 parts of pueraria flavonoid powder, 0.1 to 1.5 parts of bamboo flavonoid powder, 0.1 to 1.5 parts of semen hoveniae extract powder, 0.05 to 0.2 part of silymarin powder, 1 to 4 parts of gastrodia elata powder, 1 to 6 parts of fig powder, 0.1 to 1 part of algin powder, 0.01 to 0.2 part of menthol powder, 0.008 to 0.2 part of astaxanthin powder, 15 to 30 parts of resistant starch, and 69.8 to 184.8 parts of purified water. The Chinese medicinal alcoholism relieving health-care beverage has the advantages of mild medicinal property, delicious taste, pure nature and safety, definite content of effective ingredients and quick and remarkable drug effect, stable and consistent quality control and long term administration.

The invention discloses chromium-contaminated soil restoring fertilizer which is prepared from, by weight, 13-15 parts of straw fibers, 28-30 parts of castor cakes, 24-26 parts of animal broken bone powder, 1-3 parts of sodium tripolyphosphate, 2-3 parts of ferrous sulfate, 22-25 parts of flax fiber beams, 23-25 parts of spathic iron slag, 23-25 parts of limonite slag, 75-80 parts of orange peel, 3-4 parts of an EM compound microbial agent and a proper amount of water. According to the soil restoring fertilizer, multiple materials are adopted and matched, through the chelation and adsorption functions of sodium tripolyphosphate, charcoal and the like, the harm of heavy metal to soil is lowered, ferrous sulfate and the like are added and jointly act with microorganisms and organic matter, reduction of hexavalent chromium in the soil into nontoxic hexavalent chromium is promoted, meanwhile the content of effective nutritious elements in the soil is increased, the purpose of restoring soil contaminated by heavy metal, especially chromium is achieved, and the chromium-contaminated soil restoring fertilizer has quite good application and popularization value.

The invention discloses a phytoremediation soil auxiliary fertilizer. The phytoremediation soil auxiliary fertilizer is prepared from, by weight, 11-13 parts of toosendan fruit sawdust, 36-38 parts of rape seed cake powder, 7-9 parts of peat soil, 5-6 parts of residues of monosodium glutamate, 1-2 parts of zinc sulfate, 1-3 parts of sodium hexametaphosphate, 3-4 parts of chitosan, 28-32 parts of horseradish tree pods, 16-17 parts of siderite slags, 16-17 parts of limonite slags, 68-74 parts of orange peels, 3-5 parts of effective microorganism (EM) agents and a proper amount of water. According to the soil remediation fertilizer, the quantity of effective microorganisms in soil can be remarkably increased by addition of the plentiful carbon-source nutrients, so that degradation of organic pollutants in soil is promoted, the soil micro-ecological environment is improved, a good soil granular structure is formed, and the soil fertility recovers and is improved; and by matching of cultivation of heavy metal accumulation plants, accumulator plants can grow quickly, the heavy metal absorption and storage amount is increased, and consequently the phytoremediation efficiency is improved.

The invention discloses a soil restoration fertilizer for bettering soil fertility. The soil restoration fertilizer for bettering soil fertility is prepared from, by weight, 36-40 parts of cottonseed meal, 9-13 parts of biochemical fulvic acid, 13-15 parts of pine needle leaf powder, 2-3 parts of bacillus mucilaginosus, 1-3 parts of zeolite powder and calcium formate, 2-3 parts of sodium pyrophosphate, 33-36 parts of eucalyptus camaldulensis bark, 17-19 parts of siderite slag, 17-19 parts of limonite slag, 75-78 parts of orange peel, 6-7 parts of EM compound microbial agent and a proper amount of water. The soil restoration fertilizer has the functions of activating soil and increasing soil organic matter, the special requirements of grain produced by soil, especially saline alkali soil for micronutrient fertilizer are met, added bacillus mucilaginosus and fermented materials are collaboratively applied to soil so that the lattice structure of aluminosilicate, hard-to-dissolve phosphorus compounds and the like can be damaged, soil fertility is enhanced, crops are induced to enhance cold resistance, drought resistance, disease resistance and adverse resistance, and the quality and yield of the crops are improved.

The invention discloses a preparation method of a novel bio-magnetic suspension filler for water treatment. According to the method, a magnetic powder is activated after alkali modification, then polyurethane is subjected to copolymerization modification with an organic silicone emulsion, a dispersing lubricant, a crosslinking agent and a pore-forming agent are added for high-speed kneading to obtain a mixed material, the mixed material is directly subjected to extrusion forming and cutting, the pore-forming agent is removed by vacuum drying to make the interior of the formed filler porous toobtain a suspension filler, the suspension filler is magnetized and then loaded with a surface-enhancing component to obtain the bio-magnetic suspension filler.

The invention provides an anti-alcoholism beverage using tea saponin as a main body. The anti-alcoholism beverage comprises the following ingredients: tea saponin, extracts from pueraria, extracts from pericarpium citri reticulatae, jujube date polysaccharide, extracts from medlar, extracts from haw apple, honey, citric acid, vitamin B1, vitamin B6 and vitamin C. The disclosed anti-alcoholism beverage is high in raw material utilization ratio, low in cost and good in taste, and can protect liver and stomach. According to the anti-alcoholism beverage provided by the invention, the drunkenness endurance time of mice can be prolonged, the sleeping time is reduced, the concentration of ethyl alcohol in blood can be obviously reduced, the activity of in vivo glutathione peroxidase and the activity of superoxide dismutase after drinking are increased, the metabolism of the ethyl alcohol and the elimination of free radicals in the liver are accelerated, the generation of peroxides is reduced, the increase of malondialdehyde content caused by alcohol is inhibited, the activity of ethanol dehydrogenase is further increased to a certain extent, the metabolism of the ethyl alcohol in the liver is quickened, the damage of the ethyl alcohol on human bodies is reduced, and the anti-alcoholism beverage has good taste.

The invention provides a fermentation method for high-content 12[alpha]-hydroxysteroid dehydrogenase fermentation liquor, and belongs to the technical field of biology and medicines. the fermentationmethod comprises the following steps of: inoculating a spare recombinant escherichia coli containing a 12[alpha]-hydroxysteroid dehydrogenase expression gene into a novel sterile fermentation medium as a strain according to an inoculation amount of 2-6% of volume fraction to be cultured; and after 2h, carrying out fed-batch by a fed-batch induced medium totally for 8h. By use of the fermentation method, in a process that the spare recombinant escherichia coli containing the 12[alpha]-hydroxysteroid dehydrogenase expression gene is taken as the strain to ferment and produce the 12[alpha]-hydroxysteroid dehydrogenase (12[alpha]-HSDH), a high-nutrition low-cost novel medium is used for replacing a traditional LB (lysogeny broth) medium, the fed-batch induced medium is adopted, the bacteria OD600 (optical density) of culture bacteria is 50-60, and meanwhile, the enzyme activity of the 12[alpha]-hydroxysteroid dehydrogenase is 700-900U / L.

The invention specifically discloses Pseudomonas aeruginosa and application thereof. The accession number of the Pseudomonas aeruginosa is CGMCC No. 10844. The invention also discloses a microbial agent containing the Pseudomonas aeruginosa and a method for passivation of lead. The Pseudomonas aeruginosa provided by the invention has strong capability in passivation of lead in a water body, can finish passivation of lead within 4 to 5 h and has a maximal lead removal rate of 97.33%. As soil contaminated by lead is inoculated with the Pseudomonas aeruginosa, the dehydrogenase activity of the soil can be obviously improved, and lead can be effectively changed into a stable state from a transformable state so as to achieve the effect of lead passivation. Moreover, as the Pseudomonas aeruginosa is used for lead passivation, operation is simple, a period is short, secondary pollution is not posed and high economic benefits and social benefits are obtained.

The invention discloses a bagasse composite soil restoration fertilizer. The bagasse composite soil restoration fertilizer is prepared from, by weight, 50-55 parts of bagasse, 12-13 parts of rice straw ash, 9-10 parts of sesame-seed cake, 3-4 parts of bamboo leaf flavonoid powder, 1-2 parts of cobaltous chloride, 2-3 parts of sodium sulfate, 30-34 parts of moringa pods, 20-22 parts of siderite slag, 20-22 parts of limonite slag, 72-76 parts of orange peel, 4-6 parts of an EM compound microorganism bacterium agent and a proper amount of water. According to the bagasse composite soil restoration fertilizer, the bagasse which is a byproduct in cane sugar manufacturing serves as the main raw material, the processes of fermentation irradiation and the like are used, a large quantity of fibers in the bagasse and a charcoal base are matched to effectively absorb heavy metal elements in soil, higher economic value is achieved, a recycling method for the waste product of cane sugar manufacturing is also provided, and cyclic utilization of resources is achieved.

The invention discloses acidified soil restoration fertilizer. The acidified soil restoration fertilizer is prepared from, by weight, 18-20 parts of dead leaves, 29-31 parts of potato straw powder, 6-7 parts of loofah powder, 1-2 parts of calcium cyanamide, 3-4 parts of sodium polyphosphate, 5-6 parts of dolomite powder, 28-30 parts of red gum bark, 19-21 parts of siderite slags, 19-21 parts of limonite slags, 65-70 parts of orange peels, 3-4 parts of EM compound microorganism bacterium agent and an adequate amount of water. According to the acidified soil restoration fertilizer, the soil acidification condition can be improved, the soil fertility disorders derived from soil acidification is relieved, a soil-borne disease caused by soil acidification is controlled, calcium ions in the soil are supplemented by adding the calcium cyanamide and the dolomite powder, a calcium ion leaching phenomenon caused by the soil acidification is relieved, various microelements are supplemented adequately, and restoration of acidified soil is achieved economically and effectively.

The invention discloses an alcohol-effect-dispelling and health-maintaining plant beverage and a preparation method of the alcohol-effect-dispelling and health-maintaining plant beverage. The invention provides a plant beverage which has the alcohol-effect-dispelling and sobering-up effects and also has the health-maintaining and health functions, and a preparation method of the plant beverage. The beverage is composed of following raw materials in parts by weight: 1-10 parts of gynostemma pentaphylla, 1-7 parts of glycyrrhiza uralensis, 10-25 parts of hovenia dulcis thumb, 15-45 parts of lobed kudzuvine roots, 10-35 parts of lobed kudzuvine flowers and 1-10 parts of crataegus pinnatifida bunge. The alcohol-effect-dispelling and health-maintaining plant beverage further comprises a flavoring agent composed of white granulated sugar, citric acid, potassium sorbate and fruity essence. All the raw materials are reasonably matched and have a synergistic effect; the intestines and stomach absorption of alcohol is inhibited by effects of sweating, promoting diuresis, relaxing the bowels and the like, the first pass effect of the alcohol in gastrointestinal tracts is enhanced and the concentration of the ethanol in blood is reduced; meanwhile, medicines directly act on a liver metabolic enzyme system so as to enhance the activity of ethanol dehydrogenase, accelerate the elimination speed of the ethanol and metabolites thereof, and relieve the injuries to tissues and cells; the alcohol-effect-dispelling effect is very obvious and the sleeping time is shortened; the sobering-up recovery time is 62.3 minutes.

The invention discloses moisturizing and soil moisture preserving soil remediation fertilizer. The fertilizer is prepared from, by weight, 35-36 parts of rice straw, 6-8 parts of flammulina velutipes dregs, 10-12 parts of intestine and feather meal, 8-10 parts of bentonite, 4-5 parts of ammonium dihydrogen phosphate, 2-3 parts of sodium hydrogen sulfite, 39-41 parts of sugar beet pulp, 14-18 parts of siderite slag, 14-18 parts of limonite slag, 52-55 parts of orange peels, 3-6 parts of EM compound microbial agents and an appropriate amount of water. The organic waste residues are used as main raw materials of the soil remediation fertilizer and are reasonably matched with the bentonite, the microbial agents and the like, and physical and chemical properties of soil are improved; the organic filler of the appropriate percentage can increase the dialysis speed and the total porosity of the soil, increase the content of related saline ions, and improve the microbial activity and is beneficial to nutrient conversion and circulation and moisturizing and soil moisture preservation of the soil, and a good environment is provided for crop growth after remediation.

The invention discloses lead zinc ore contaminated soil remediation fertilizer which is prepared from, by weight, 3-5 parts of bacillus conidial powder, 26-30 parts of grass carbon powder, 40-45 parts of wheat bran, 8-10 parts of dregs of tea-oil tree fruits, 3-5 parts of citric acid, 2-3 parts of zinc sulfate powder, 30-35 parts of pinewood, 17-18 parts of siderite slag, 17-18 parts of limonite slag, 67-73 parts of orange peel, 4-6 parts of EM compound microorganism bacterium agents and a proper amount of water. According to the soil remediation fertilizer, for the content of heavy metal, especially the content of lead and zinc, in the lead zinc ore contaminated soil, through the synergistic effect of organic matter and bactericide in the fertilizer, the plant availability of heavy metal, such as lead and zinc is passivated, the toxicity of the lead element and the zinc element in the soil of the plant roots is relieved, meanwhile the organic content of the contaminated soil is greatly increased in match with the grass carbon powder and the dregs of tea-oil tree fruits, breeding of indigenous microorganism in the soil is promoted, and the self purification capacity of the soil is improved.

The present invention relates to a microorganism with improved production of 5′-xanthosine monophosphate and 5′-guanine monophosphate, and more specifically, to a Corynebacterium sp. microorganism having increased proline dehydrogenase activity compared with an intrinsic activity thereof, a method for producing 5′-xanthosine monophosphate or 5′-guanine monophosphate from the culture medium obtained by culturing the transformed microorganism, and a use of the microorganism for production of 5′-xanthosine monophosphate or 5′-guanine monophosphate.

The invention provides a composition for dispelling the effects of alcohol and protecting the liver and application thereof, and relates to the technical field of health care products. The compositioncomprises a water extract of medicinal and edible traditional Chinese medicines, probiotics, taurine, vitamin B and vitamin C. The composition is prepared from medicinal and edible crude drug raw materials specified by Ministry of Health and nutrition enhancer raw materials conforming to national standards of national food raw materials, has both treatment and health care effects, can be preparedinto health care products which have corresponding functions and can be eaten for a long time, and has the effects of dispelling the effects of alcohol immediately and protecting the liver after being taken for a long time.

The invention relates to a soil amendment which is prepared from, by weight, 50-150 parts of fly ash, 100-200 parts of livestock manure, 100-200 parts of active sludge, 150-250 parts of leguminous green manure and 10-30 parts of mushroom bran. Compared with the prior art, the soil amendment has the advantages that soil aggregation is promoted, soil physical and chemical properties are improved, soil fertility is improved, and the yield of crops is increased.

The present invention is a system capable of increasing retinal and neural glucose oxidation by enhancing pyruvate dehydrogenase activity and therefore treats retinopathy and central nervous system disorders in both diabetic and non-diabetic patients. The current invention is a system of the treating of eye and nerve diseases using insulin pulses to a patient utilizing Chronic Intermittent Intravenous Insulin Therapy to achieve an increase in retinal and neural glucose oxidation by enhancing pryuvate dehydrogenase activity, therefore treating retinopathy and central nervous system disorders in both diabetic and non-diabetic patients.

The invention relates to the technical field of ethanol detection of blood serum and in particular relates to an enzyme-method ethanol (ALC) detection reagent with a high anti-interference capability and high accuracy. A reagent R1 is mainly prepared from a buffering solution, ammonium oxalate, hydrazine hydrochloride, potassium nitroprusside, an ion balancing agent, ascorbic acid oxidase, a protecting agent, a surfactant, NAD (Nicotinamide Adenine Dinucleotide) and a preservative; a reagent R2 is mainly prepared from a buffering solution, a protecting agent, a heavy meal ion chelating agent, alcohol dehydrogenase and a preservative. The ammonium oxalate and the hydrazine hydrochloride are added into the reagent R1 so that interference caused by lactic dehydrogenase and hydroxybutyrate dehydrogenase can be effectively removed; less potassium nitroprusside is added so that interference caused by reducing substances including bilirubin and the like is effectively removed, so that the anti-interference capability of the reagent is greatly improved; the ascorbic acid oxidase is added so that interference caused by ascorbic acid can be effectively removed; the heavy metal ion chelating agent aminotriacetic acid is added so that interference caused by heavy metal ions, especially calcium ions, on dehydrogenase activity is removed very well, so that the activity of the alcohol dehydrogenase is improved, and the reagent becomes one detection reagent with the high anti-interference capability and the high accuracy.

The invention discloses fertilizer for repairing soil contaminated by snow-melting agents. The fertilizer is prepared from, by weight, 30-34 parts of mushroom bran, 8-12 parts of seaweed meal, 14-16 parts of cattle bone powder, 10-12 parts of bean pulp, 6-7 parts of sepiolite powder, 3-4 parts of pyroligneous liquor, 2-3 parts of copper sulfate, 36-38 cow dung, 16-18 parts of siderite slag, 16-18 parts of limonite slag, 60-65 parts of orange peel, 4-6 parts of EM compound microorganism bacterium agents and a proper amount of water. According to the fertilizer for repairing soil, for soil hardening and salinization caused by inorganic snowmelt agents which are current used, the breathability and water binding capacity of soil are improved through adding of organic materials, salt in the snow-melting agents is adsorbed and neutralized in match with the sepiolite powder, the pyroligneous liquor and the like, contamination of underground water is eliminated, meanwhile composition of soluble salt is adjusted, the fertility of the contaminated soil is enhanced, and the fertilizer has important practical value on the aspect of soil repair of green belts along highways.

The invention discloses a repair fertilizer capable of loosening soil. The fertilizer capable of loosening the soil is prepared from, by weight, 8-9 parts of clay, 14-15 parts of tobacco leaves, 12-13 parts of turnip dregs, 25-28 parts of biogas fluid, 6-7 parts of granulated sugar, 2-3 parts of stannous chloride, 38-42 parts of cattle manure, 9-12 parts of siderite slags, 9-12 parts of limonite slags, 48-52 parts of orange peels, 3-4 parts of EM compound microbial agent, 3-5 parts of sodium silicate and an appropriate amount of water. According to the repair fertilizer capable of loosening the soil, on one hand, the using effect of the fertilizer is achieved through addition of organic matter, and the soil fertility is improved; on the other hand, the clay and silicate such as the sodium silicate are added, an ion substitution reaction can be conducted on the soil and the silicate and inorganic salt which is fixedly contained in the soil, a rigid pillared structure which is of a porous structure is generated, the soil structure becomes loosened, and the effect of repairing the soil is further achieved.

The invention discloses a biological soil restoration fertilizer. The fertilizer is prepared from, by weight, 32-36 parts of biogas slurry, 4-5 parts of saccharomyces cerevisiae, 6-9 parts o brown sugar, 3-4 parts of calcium powder, 1-2 parts of copper sulphate pentahydrate, 26-30 parts of flax fiber bundles, 15-17 parts of siderite slag, 15-17 parts of limonite slag, 68-70 parts of orange peels, 3-6 parts of an EM compound microbial agent and a defined amount of water. By means of the biological soil restoration fertilizer, biological activity of soil can be effectively improved, growth and expanding of plant roots are facilitated, water permeability and air permeability of the soil are improved, the soil is prevented from being eroded by bacteria and pests, healthy growth of plants is promoted, meanwhile, heavy metal can be subjected to a precipitation reaction, a chelation reaction and other reactions in the soil, bio-availability of the heavy metal is lowered, the absorbing capacity of the crops for the heavy metal is reduced, quality of agricultural products is improved, and the ecological environment of the soil is restored.

The invention discloses a soil remediation fertilizer for newly-exploited farmland of a mining area. The soil remediation fertilizer is prepared from, by weight parts, 44-48 parts of bagasse, 9-11 parts of fish bone meal, 6-7 parts of river sand, 2-4 parts of calcium chloride, 1-2 parts of aluminum potassium sulfate, 1-3 parts of magnesium oxide, 50-55 parts of bombax ceiba shell, 16-18 parts of siderite residue, 16-18 parts of limonite residue, 37-41 parts of orange peel, 5-6 parts of EM compound microorganism bacterium agent and an appropriate amount of water. According to the soil remediation fertilizer, according to the characteristics of high heavy metal and dust content in the newly-exploited farmland of the mining area, pollution adsorption is performed through highly active magnetic charcoal; the content of organic humus ingredients in the soil is greatly improved after the fish bone meal is become thoroughly decomposed, a physicochemical state of the farmland soil is improved, the soil fertility is improved quickly, and the productivity of the newly-exploited farmland is improved.

The invention discloses a long-acting carbon-reducing soil restoration fertilizer which is prepared from, by weight, 12-15 parts of tobacco powder, 6-7 parts of pine pollen, 38-40 parts of plant ash, 6-7 parts of boronic acid, 1-2 parts of sodium sulphide, 1-2 parts of sodium bismuthate, 26-28 parts of deal, 14-16 parts of siderite slag, 14-16 parts of limonite slag, 74-76 parts of orange peel, 4-5 parts of an EM composite microbial agent and a proper amount of water. According to the long-acting carbon-reducing soil restoration fertilizer, a structure of biochar solid-supported organic matter is adopted, the fertilizer has good corrosion resistance and can form aggregate together with mineral substances in soil, the function of microorganisms on biochar is weakened, and the fertilizer can stay in the soil for a long time. The surface adsorbs dissolvable organic carbon to promote formation of soil particles wrapped by organic matter, the mineralization function of the organic carbon of the soil is reduced, emissions of CO2 are reduced, and the aims of soil restoration, carbon sequestration and emission reduction are achieved.