98 results about "Blood cell" patented technology

The application discloses a method for establishing a mouse model of MHC haplotype compatible allogeneic hematopoietic cell transplantation, and belongs to the technical field of animal experiment model construction. The technical problem to be solved by the application is how to construct a mouse model which can be used to study the immune reconstruction mechanism and treatment effect evaluation of MHC haplotype compatibility and MHC full compatibility in bone marrow cell transplantation recipient mice. To solve the technical problem, the application provides a method for constructing a mouse model of MHC haplotype compatible allogeneic hematopoietic cell transplantation, which comprises transplanting bone marrow cells of a donor mouse into a recipient mouse, and the MHC haplotype of the donor mouse and the recipient mouse is compatible. The application sets a modeling condition, and the model establishment condition is evaluated by survival condition, graft-versus-host disease and rejection reaction observation, flow cytometry and histopathological section, so as to prove that the model is reliable and stable.

The application discloses a kind of based on DNA methylation's construction method of biological age prediction model, the application is downloaded from GEO data website, derived from Chinese population, contains calendar age data, whole blood sample 450k methylation chip data, by the method of elastic network combined bootstrap, 31 methylation sites of modeling candidate are screened, multiple linear regression, support vector machine, random forest and gradient boosting regression tree are used to carry out preliminary construction and evaluation of model, then further filter methylation sites using full subset regression, obtain methylation age prediction model based on 18 methylation sites.Finally, using any provincial team natural population data, methylation age prediction model is optimized, and finally the biological age prediction model based on 18 methylation sites is obtained.The model is suitable for Chinese population, the number of methylation sites contained is less, is not affected by blood cell components, and the prediction accuracy is good.

This application provides a method for using a POCT hematology analyzer, comprising: in response to a reagent kit being placed in a test chamber, controlling a pipette to move above the first mounting head reservoir of the reagent kit and descending to load the first mounting head; controlling the pipette to move to the diluent reservoir of the reagent kit to transfer the diluent into the pre-cell for impedance detection; controlling the pipette to transfer a sample into the pre-cell; mixing the test solution in the pre-cell; controlling the metal shielding cap to close with the test chamber to establish a connection between the rear cell and the pressure building system of the reagent kit; controlling the pressure building system to guide the test solution from the pre-cell into the rear cell; and detecting the test solution by impedance detection.

1. Name of the design product: bracelet (blood cell separation sheet). 2. Use of the design product: the design product is a bracelet worn on the wrist, which activates health conditions naturally through physical therapy in a non-drug and non-chemical way. 3. Design points of the design product: in the combination of shape and pattern. 4. Picture or photo best indicating the design points: perspective view 1.

A filter device according to the present invention is to be mounted on a centrifugal separator and includes: a first space which is formed in a housing and into which blood flows; a second space which is formed in the housing and from which blood flows out; a partition wall that separates the first space and the second space and that is formed with a communication port establishing communication between the first space and the second space; and a white-blood-cell removal filter that is disposed in the second space. The first space is located between the partition wall and a first main surface of the housing, and the second space is located between the partition wall and a second main surface of the housing.

The application belongs to the technical field of cell culture, and particularly relates to a method for determining the number of particles in a unit volume of liquid and application. The method comprises the following steps: (1) using a dye to dye the liquid to be measured, and sequentially dropping the dyed liquid to be measured and a chromatographic separation liquid at one end of filter paper, the particles in the liquid to be measured are dispersed on the filter paper under the action of capillary chromatography, and the number of particles in the liquid to be measured is obtained by naked-eye counting; (2) according to the volume of the liquid to be measured, the number of particles in a unit volume of liquid is calculated. The determination method of the application does not need to purchase expensive instruments, does not need to take photos and use image analysis software for auxiliary counting, breaks through the limitation that a blood cell counter cannot determine particles with a particle size greater than 100 mu m, and can complete the determination of the number of particles by naked-eye observation, which is simple, efficient and low in cost.

This utility model belongs to the technical field of blood cell analyzers, specifically relating to an isolation chamber device for a blood cell analyzer. It includes an isolation chamber body, a waste liquid outlet fixedly connected to the lower outer side of the isolation chamber body, a mixing gas inlet fixedly connected to the upper outer side of the isolation chamber body, a top cover fixedly connected to the upper side of the isolation chamber body, a reaction tank connection port fixedly connected to the upper side of the top cover, a filter body fixedly connected inside the isolation chamber body, a fixed blocking part fixedly connected inside the isolation chamber body opposite to the mixing gas inlet, a buoyancy component and a movable blocking part connected to the buoyancy component, which, when the buoyancy component rises, moves an airbag to the mixing gas inlet to seal it. This utility model can reduce the phenomenon of liquid from inside the isolation chamber body entering the mixing gas inlet.

The present invention provides a method for manufacturing iPS cells. The method uses a cell processing apparatus having a closed system consisting of a sealed container for material supply and a rotatable sealed chamber for centrifugation connected by connecting pipes. While maintaining the airtightness of the closed system, the following steps are performed sequentially within the rotatable sealed chamber: centrifuging blood cells from whole blood; contacting initialization factors with the aforementioned blood cells; and establishing iPS cells from the aforementioned blood cells.

According to the automatic detection method and device for the blood cells in the blood smear image, a local teacher model and an external teacher model are combined to generate high-quality pseudo labels for original extremely sparse labeled data, the models are guided to learn a complete foreground concept, and the problem of a large amount of missing detection caused by insufficient labeling is effectively relieved; the text feature of the blood cell category name is used as a domain invariant anchor point, a detector is guided to generate visual representation with strong generalization, and excellent generalization performance is achieved in multiple visual domains; inter-text comparative learning and inter-visual comparative learning are introduced to promote clearer alignment between text features and visual features from multiple dimensions, and generalization is improved while discrimination is guaranteed.

The application provides a method for constructing a PCI postoperative patient all-cause death risk prediction model, which comprises the following steps: obtaining a training data set containing clinical electronic medical records, blood cell count and blood biochemical data of patients undergoing selective PCI, and at least 5 years of postoperative follow-up and all-cause death label; performing Z-score standardization on continuous variables and one-hot encoding on category variables for pretreatment; screening 43 core features through LASSO regression combined with ten-fold cross-validation; constructing a model based on a LightGBM algorithm, taking a binary logarithmic loss function as a measurement standard and optimizing hyperparameters through grid search; finally outputting a prediction model which can receive core feature data of a new patient and output the postoperative all-cause death probability of the new patient; and the application can realize accurate prediction of long-term death risk of PCI postoperative patients.

A flushing system configured to flush a wound and / or a body cavity of a patient comprises: an infusion liquid outlet to connect a first tube guiding a flow of infusion liquid from the system to the wound and / or the body cavity; an effusion liquid inlet to connect to a second tube guiding the effusion liquid flow from the wound and / or the body cavity to the system; a flow rate control system to control the flow rate at the infusion liquid outlet; and a hematocrit sensor configured to optically measure the hematocrit value of the effusion liquid received via the effusion liquid inlet, to obtain a blood loss volume and / or a blood loss flow rate from the wound and / or the body cavity by means of emitting light having different wavelengths. The different wavelengths are respectively assigned to the green wavelength range and to the red or near-infrared wavelength range.

This application discloses a blood cell washing centrifuge for use in the field of medical devices. The application includes: a centrifuge housing, a centrifugation mechanism, a signaling mechanism, a mixing mechanism, a cleaning mechanism, and a liquid storage chamber. The centrifugation mechanism is disposed inside the centrifuge housing and includes a centrifuge support frame and a centrifugation device, the centrifugation device being mounted on the centrifuge support frame. The signaling mechanism is disposed inside the centrifuge support frame and is used to control the cleaning mechanism to perform the washing operation. The cleaning mechanism is mounted on the centrifuge support frame, and a pipeline is disposed below the cleaning mechanism connecting to the liquid storage chamber for collecting waste liquid and injecting washing liquid during the washing operation. The mixing mechanism is disposed below the centrifuge support frame for mixing the centrifuged sample. This application can solve the problems of cumbersome operation, long time consumption, errors caused by manual operation, and high manpower consumption during the washing process.

The utility model relates to medical equipment technical field discloses a kind of platelet-rich plasma separators, comprising: round plate, the outer surface of the round plate is fixedly installed with limit block.This kind of platelet-rich plasma separator, due to the design of movement mechanism, reach the flexible adjustment effect of centrifugal radius without replacing centrifugal cup, can be according to different patient blood viscosity, hematocrit and other indexes, accurately control centrifugal force, avoid platelet residue or excessive activation, significantly improve platelet-rich plasma preparation quality and biological activity.Discard the cumbersome operation of traditional replacement centrifugal cup, substantially improve operation efficiency, while reducing the mismatch risk caused by frequent replacement of spare parts, reduce the operation burden of medical staff, reduce equipment spare parts procurement cost and management difficulty, effectively improve the versatility and utilization of equipment, reduce hospital equipment procurement and maintenance cost.

This application relates to a blood cell analyzer and a red blood cell detection method. The blood cell analyzer includes: a sampling device for aspirating a blood sample to be tested; a sample preparation device for mixing a portion of the blood sample to be tested with a diluent to prepare a test sample; an impedance detection device including a counting cell and a detection component, wherein the counting cell is for the test sample to pass through, and the detection component is for acquiring the electronic signals of each particle in the test sample as it passes through the counting cell; and a processor configured to: acquire raw red blood cell volume distribution information based on the electronic signals of each particle in the test sample; acquire red blood cell agglutination information based on the raw red blood cell volume distribution information; and obtain the red blood cell count value of the blood sample to be tested based on the raw red blood cell volume distribution information and the red blood cell agglutination information. This enables the acquisition of an accurate red blood cell count value.

We disclose here the nano-enabled delivery of highly potent 5-hydroxymethyl furfural (5-HMF) to sickled blood cells in patients. 5-HMF, a superior antisickling agent, has been formulated to address the limitations of existing treatment modalities arising from disfavorable pharmacokinetics of the drug compound. Specifically, two complementary 5-HMF prodrugs are integrated as a ‘protected’ biocompatible composite nanoparticle designed to readily fuse with RBCs and be amenable to transdermal delivery (5-HMF-grafted-phospholipid layered over a sucrose-derived graphitic carbon dot core). These RBC-targeted, protected, biocompatible, self-assembled 5-HMF prodrug nanoparticles for sickle cell disease are the first in class technology for offering a treatment for sickle cell disease which has improved potency, targeted payload delivery, and extended release with the red blood cells with enhanced pharmacodynamic effect in a treated patient.

Compositions, methods, and uses are contemplated in which a betalain-containing preparation is administered to a mammal to enhance athletic performance. Most preferably, the preparations are substantially nitrate free and acutely and transiently increase hematocrit, erythropoietin, adrenalin, and beta endorphin, while reducing serum lactate, serum lactate dehydrogenase, heart rate and a rate of perceived exertion.

Disclosed are methods, materials and devices for approximation of blood volume in a fluid, such as in a biological fluid collected during a surgical procedure. The method and devices may include the use of an RBC flocculant, for example polyDADMAC, and an approximate blood hematocrit from the type of animal blood being evaluated, as well as a calculated RBC packing ratio corresponding to the collection device being used. Also provided is a Blood Indicator Panel (BIP), comprising a series of markings calculated from an observed red blood settlement volume, the average animal blood hematocrit, and a calculated RBC packing ratio “η” value for the collection device. A collection device with a BIP is disclosed. Pediatric (about 200 ml or 250 ml size container), adult human (about 1,000 ml-1,500 ml) and veterinary (about 500 ml-2,500 ml) collection containers are also disclosed, that include a RBC flocculant, for use in approximating blood volume in a fluid.

The application discloses atherosclerotic cardiovascular disease patient risk assessment based on blood microorganisms, and further discloses a atherosclerotic cardiovascular disease prediction system, which comprises a sampling and layering module, a microorganism sequencing module, an index detection module and a data analysis module. The sampling and layering module divides samples into four groups, i.e., old men, old women, middle-aged men and middle-aged women, according to age / sex, and each group contains more than 100 ASCVD patients and more than 50 healthy controls. The microorganism sequencing module generates equal-length fragments by digesting DNA through BcgI enzyme digestion, connects the equal-length fragments through a linker, amplifies the equal-length fragments through PCR, and constructs a microorganism tag database (2b-Tag-DB) through Illumina sequencing. The index detection module analyzes traditional risk indexes such as blood cell classification, blood lipids and inflammatory factors. The data analysis module calculates the microorganism Gscore value, screens Gscore>5 species, and carries out diversity analysis in combination with Chao1 / Shannon index and UniFrac / MetaStorms algorithm. The prediction model module integrates the microorganism characteristics and the traditional indexes to generate a risk prediction result. The system significantly improves the ASCVD risk prediction accuracy through the collaborative analysis of the microbiome and the clinical indexes.

This invention describes a method for preparation of stable hematology reference materials by producing synthetic hydrogel blood cell surrogates, which mimic human blood components in size, morphology, performance and functionality when analyzed using an automated hematology analyzer employing multiple detection technologies. Different hydrogel particles can be combined and mixed to prepare multi-parameter and multi-level hematology reference materials, which could be used for calibration, linearity verification, proficiency evaluation, and routine performance monitoring of modern automated hematology analyzers. These hydrogel particles can also be combined with processed and stabilized human blood components to prepare the reference materials of this invention.

A method for collecting blood components using a medical system, the method comprising: receiving data associated with a subject by the medical system, the data associated with the subject including the subject's initial hematocrit value; determining the amount of subject-specific pure component to be collected by the medical system, the amount of subject-specific pure component to be collected by the medical system being determined using the subject's hematocrit value, the subject's total blood volume, and a target change in the hematocrit value; and performing a component collection process using the medical system to collect the determined amount of subject-specific pure component.

This invention relates to the field of hematology analysis technology, and in particular to a diluent for hematology analysis, its preparation method, and its uses. The diluent comprises the following components: 0.5-15 g / L of inorganic salt, 0.01-5.0 g / L of preservative, and 0.1-0.5 g / L of stabilizing agent; the solvent of the diluent is water; the diluent also includes an anti-precipitant, selected from one or more of salt-based and organic anti-precipitants; the salt-based anti-precipitant includes one or more of thiocyanate and nitrate, with a content of 2.0-5.0 mmol / L; the organic anti-precipitant includes one or more of trehalose, mannitol, and amino acid ionic liquids, with a content of 4.0-8.0 mmol / L. In this diluent, the components work synergistically to reduce the maintenance cost of the hematology analyzer, increase its service life, and improve detection efficiency.

The application discloses a full-spectrum analysis method for monitoring blood oxygen saturation and hematocrit in an extracorporeal circulation pipeline, and comprises the following steps: step 1, constructing a photoelectric acquisition device; step 2, performing system initialization calibration; acquiring CMOS image sensor data, photodiode sensor dark current calibration data and empty pipeline reference data; step 3, using a main control unit to control a mixed light source module and a mixed sensor module to perform time sequence synchronous acquisition; acquiring a transmission image of a visible light band and a transmission light intensity voltage of an infrared band at a fixed frame rate; step 4, pre-processing the acquired transmission image data and transmission light intensity voltage data to generate an optical characteristic map reflecting the pipeline and an optical density vector reflecting absolute light absorption; and step 5, inputting the pre-processed optical characteristic map and optical density vector into a pre-trained double-flow deep neural network model to obtain quantitative values of blood oxygen saturation and hematocrit of liquid in the pipeline.

The embodiments of the present application relate to a platelet counting method and a blood cell analyzer. The method comprises: aspirating a blood sample to be tested; mixing one portion of said blood sample with a diluent to prepare a first test sample, and mixing the other portion of said blood sample with a hemolytic agent and fluorescent dye to prepare a second test sample; performing impedance measurement on the first test sample, in order to obtain an electrical signal of the first test sample; performing optical measurement on the second test sample, in order to obtain at least one optical signal from among a scattered light signal and a fluorescent light signal of the second test sample; at least on the basis of the electrical signal of the first test sample, obtaining platelet distribution information of said blood sample; and at least on the basis of the pulse width of an optical signal among the at least one optical signal that corresponds to platelets in the second test sample, and the platelet distribution information, obtaining a platelet count value. Thus, an accurate platelet count can be obtained at a low cost.

This utility model provides a reagent bottle assembly and a blood cell analyzer incorporating the same. The reagent bottle assembly, used in the blood cell analyzer, includes: a reagent bottle body; a flexible tube with an input end and an output end, the input end being located inside the reagent bottle body and the output end being located outside the reagent bottle body; and a suction head located inside the reagent bottle body and positioned at the input end. A first end of the suction head is connected to the input end, and a second end of the suction head has an opening. The suction head has a suction channel connecting the input end and the opening. The center of gravity of the suction head is located on the side of a preset plane away from the input end, wherein the preset plane is half-height plane of the suction head. The technical solution of this application effectively solves the problem of poor suction effect in related technologies.

The application provides tooth extraction socket filling hemostatic material and a preparation method thereof, and relates to the technical field of tissue repair materials.The tooth extraction socket filling hemostatic material provided by the application comprises the following components in parts by mass: bioactive ceramic powder 0.1-10 parts, glycerol 0.1-4 parts, starch 2-10 parts, solidification liquid 0.01-3 parts and plasticizing agent 0.01-5 parts.In the tooth extraction socket filling hemostatic material, the molecular chain of the starch is significantly stretched after the starch is infiltrated by glycerol rich in hydrogen bonds, so that the amount of exposed hydroxyl groups is increased, the recruitment of blood cells is accelerated, the formation of blood clots is accelerated, and the adhesion strength of the material to the tissue is significantly increased.The addition amount of the bioactive ceramic powder, glycerol, starch, solidification liquid and plasticizing agent is controlled, so that the tooth extraction socket filling hemostatic material has the function of plasticity, rapid hemostasis can be realized, the wet adhesion is good, and stability can be maintained in the oral environment.

This invention discloses a targeted plasma preservation solution for the auxiliary detection of lung cancer ctDNA. The invention utilizes biomimetic nanovesicles to achieve targeted and controlled release of free radical scavengers, preventing their degradation during shelf life; it inhibits blood cell metabolism through multiple pathways by combining metabolic inhibitors; it efficiently protects cell membrane integrity using polycarboxybenzene methyl methacrylate (PCBMA); and it protects short DNA fragments with spermine, significantly improving the capture and preservation efficiency of ultra-short ctDNA from lung cancer. The blood collection tubes of this invention have a long shelf life, effectively inhibit hemolysis and genomic DNA contamination, and can stably recover lung cancer ctDNA after 7-14 days of storage at room temperature, significantly improving the detection rate of mutations in driver genes such as EGFR. It is suitable for early lung cancer screening, auxiliary diagnosis, and efficacy monitoring.

The application provides a high-efficiency expansion culture method of umbilical cord blood gamma delta T cells and application thereof, and belongs to the technical field of biological medicine. Through a specific two-stage strategy of "initial induction culture + expansion culture", the application is simple in operation, stable in system, and high in success rate, effectively overcomes the defects of limited autologous blood source, unstable expansion and low cell activity in the prior art, and lays a foundation for large-scale preparation and allogeneic application of gamma delta T cells. The umbilical cord blood gamma delta T cells obtained by the method of the application are excellent in quantity, purity, activity and anti-tumor function, and have stronger tumor cell killing ability, and are especially suitable for adoptive immunotherapy. The application breaks through the technical bottleneck of the traditional culture system, provides a high-efficiency, stable and clinically convertible gamma delta T cell expansion scheme, and has important application prospect and market value.