321 results about "Blood cell" patented technology

A blood cell analyzer (100) and a blood cell analysis method (300). The blood cell analysis method (300) comprises: sucking a blood sample to be tested; preparing a first determination sample and obtaining first optical information; preparing a second determination sample and obtaining second optical information; generating a first leukocyte classification scatter diagram and a second leukocyte classification scatter diagram; determining a first counting result of neutrophils of the to-be-detected blood sample based on the first leukocyte classification scatter diagram and the second leukocyte classification scatter diagram; determining an actual distribution area of neutrophils from the first leukocyte classification scatter diagram; based on a reference distribution region of neutrophils of a reference leukocyte classification scatter diagram of the normal blood sample, determining a leaf-division nucleophore distribution region from the actual distribution region; determining a second counting result of the cells falling into the leaf-separating nucleogranulocyte distribution area; and based on the first counting result and the second counting result, determining a counting result of the rod-shaped nuclear granulocytes in the to-be-detected blood sample.

The invention relates to the field of plasma separation and detection, in particular to a composition for separating plasma and a using method thereof. The composition comprises: a phase change component, which is selected from n-alkanes, which are selected from at least one of n-tetradecane, n-pentadecane, n-hexadecane, n-heptadecane, n-octadecane, n-nonadecane, n-eicosane, n-heneicosane or n-docosane; a liquid phase carrier component, wherein the liquid phase carrier component is selected from silicone oil; the structural framework and rheology regulation and control component comprises fumed silica, and the structural framework and rheology regulation and control component comprises fumed silica; the dispersion and stabilization auxiliary agent component is selected from at least one of TiO2 and BaSO4, the density adjusting filler component is selected from at least one of TiO2 and BaSO4, and the dispersion and stabilization auxiliary agent component comprises a silane coupling agent. According to the device, plasma and blood cells can be reliably, rapidly and effectively separated only through one-time low-temperature centrifugation, and remixing caused by transportation jolting can be avoided.

The present invention relates to systems and methods for optimizing plasma collection volume. A plasma withdrawal system and a method for operating a plasma withdrawal system are provided by which the volume / weight of the anticoagulated plasma collected is optimized. In one example, a nomogram is provided that utilizes the donor hematocrit to calculate the volume / weight of raw plasma within a plasma product having a maximum volume allowed by the FDA nomogram. In a plasma withdrawal process having multiple collection phases followed by a reinfusion cycle in which concentrated red blood cells are returned to the donor, the volume of plasma product to be collected is calculated prior to the start of each collection cycle to account for the increasing hematocrit of the donor, resulting in a greater total volume of plasma product to be collected during the plasma withdrawal process.

The invention discloses a method for preparing an amino acid water-soluble fertilizer by processing animal blood, which belongs to the technical field of water-soluble fertilizer preparation, and comprises the following steps: step 1, collecting animal blood, and performing centrifugal treatment to remove part of impurities and blood cell fragments in the blood to obtain a relatively pure plasma part; 2, compound protease is added into the pretreated animal blood for an enzymolysis reaction, meanwhile, sodium selenite is added into an enzymolysis system, and the sodium selenite serves as a selenium source; 3, after enzymolysis is completed, a compound microbial agent is added into enzymatic hydrolysate, and aerobic fermentation is carried out; according to the method for preparing the amino acid water-soluble fertilizer through animal blood processing, the structural design is reasonable, the added selenium element can enhance the stress resistance of plants and improve the quality of agricultural products, and an acidolysis method or an enzymolysis method and a liquid fertilizer or solid fertilizer production mode are flexibly selected, so that the optimal economic benefits and social benefits are achieved.

Methods and devices for analyzing a sample from a subject are provided. A device includes one or more coherent light sources and one or more partially coherent light sources. The light sources are configured to emit first and second wavelengths of electromagnetic radiation towards an imaging chamber configured to hold blood cells. The light sources are aligned to illuminate a single common area of the imaging chamber. The device includes optics positioned to receive light from the imaging chamber, and a detector in optical communication with the optics. The detector is configured to detect fluorescence emission and backscatter from blood cells when present in the imaging chamber. The device includes mixers / unmixers configured to replicate and mix first and second signals representative of the responses to the first and second wavelengths after interacting with the plurality of blood cells to generate spectro-spatial responses.

The invention discloses an image processing method and system for zebra fish flowing blood cell recognition. According to the method, aiming at the problems of cell blurring, stretching and overlapping caused by long-time exposure in microscopic imaging, a complete process including image preprocessing, Transform-based target detection model training and self-adaptive post-processing is constructed. The preprocessing improves the image quality by enhancing the cell edge and the contrast ratio; the detection model performs end-to-end localization on cells by using an improved DETR structure; and in post-processing, an optimal threshold value is automatically selected according to different scenes so as to obtain a stable counting result. According to the method, high recognition precision can be achieved under the complex deformation condition, the good experiment condition generalization ability is achieved, and the method can be used for high-throughput zebra fish hemodynamics research and other scenes.

A blood separation device includes a pump system, a separator, and a controller. The controller is programmed to execute a blood separation procedure including a red blood cell collection stage in which blood is conveyed into the separator, red blood cells are separated from the blood in the separator, and the separated red blood cells are conveyed from the separator into a collection container. The red blood cell collection stage is ended when a target volume of blood has been separated in the separator. The controller then determines a percentage of the red blood cells from the target volume of blood that have been collected in the collection container based on the volume of red blood cells and the hematocrit of the fluid in the collection container, a volumetric flow rate of pure red blood cells in the blood conveyed into the separator, and the procedure time.

The invention discloses a multispectral microscope blood cell automatic classification and counting system. The system is composed of a multispectral illumination and microscopic imaging module, a spectrum and geometric calibration module, a multispectral preprocessing and cell segmentation module, a multispectral discrimination index and spectral band weight adaptive updating module, a cell graph structure classification module, a man-machine interaction module, an online adaptive learning module and the like. The method comprises the following steps: acquiring a blood smear image by a system under a narrow-band multi-spectrum condition, constructing cellular spectrum-morphological characteristics by combining a multi-scale segmentation result after noise suppression, flat field correction and background deduction, generating a discrimination index with a self-adaptive spectrum band weight, and completing joint classification and counting on a cell map; and meanwhile, carrying out constrained increment updating on the spectral band weight and the classification model by utilizing an artificial correction result of the low-confidence-coefficient cells. Compared with a traditional single-channel microscopic imaging and static classification method, the method has higher classification accuracy and counting stability under different dyeing and imaging conditions, and the workload of manual recheck can be reduced.

The invention discloses a method for establishing a hemophagocytic syndrome in-vitro cell disease model, and belongs to the technical field of biology. Based on human PBMC construction, the reaction of a human immune system can be truly reflected, experimental result deviation caused by species difference in an animal model is avoided, and a more accurate model basis is provided for research of human diseases. In addition to T cell over-activation, the HLH model of the present application has a clear key feature of NK cell reduction, which is HLH, accompanied by up-regulation of cytokines such as interferon-gamma and IL-10. The establishment process of the model is rapid and efficient, the experimental period is remarkably shortened, a large number of samples or drugs can be rapidly screened, the research efficiency is improved, and the conversion process from basic research to clinical application is accelerated. The method can be used for researching various mechanisms related to the HLH, is beneficial to more comprehensive understanding of pathogenesis of the HLH, and provides a theoretical basis for developing preliminary screening of targeted drugs.

This invention relates to a method and system for cardiovascular disease risk assessment based on machine learning models. By utilizing machine learning models to focus on predicting the risk of heart failure, it accurately distinguishes between healthy individuals and those with cardiovascular disease, emphasizing the differentiation of heart failure and other common cardiovascular diseases, and deeply exploring diagnostic indicators, thus opening new avenues for cardiovascular diagnosis and treatment. It hierarchically utilizes features closely related to blood cells, blood lipids, and blood glucose, and constructs predictive models using multiple machine learning models, laying a solid foundation for further in-depth research. This invention fully utilizes diagnostic indicators and multiple machine learning models, making full use of the predictive results and differentiated characteristics of different machine learning models to achieve efficient and accurate 5-category risk assessment.

The application discloses a method for establishing a mouse model of MHC haplotype compatible allogeneic hematopoietic cell transplantation, and belongs to the technical field of animal experiment model construction. The technical problem to be solved by the application is how to construct a mouse model which can be used to study the immune reconstruction mechanism and treatment effect evaluation of MHC haplotype compatibility and MHC full compatibility in bone marrow cell transplantation recipient mice. To solve the technical problem, the application provides a method for constructing a mouse model of MHC haplotype compatible allogeneic hematopoietic cell transplantation, which comprises transplanting bone marrow cells of a donor mouse into a recipient mouse, and the MHC haplotype of the donor mouse and the recipient mouse is compatible. The application sets a modeling condition, and the model establishment condition is evaluated by survival condition, graft-versus-host disease and rejection reaction observation, flow cytometry and histopathological section, so as to prove that the model is reliable and stable.

The present disclosure provides compositions and methods for the treatment and prevention of macrophage activation syndrome (MAS) or hemophagic lymphocyte hyperplasia (HLH). The methods involve administering to a patient in need thereof an antibody or fragment thereof that is specific for human GM-CSF protein.

A method of producing hematopoietic stem / progenitor cells (HS / PCs) and blood cells from adipose derived mesenchymal stem cells (Ad MSCs). The method comprises obtaining biological cells of a subject; expanding the biological cells; obtaining a pure Ad MSCs cell line from the expanded biological cells; converting the pure Ad MSCs cell line into HS / PCs in vitro; expanding the converted HS / PCs in vitro; and obtaining the expanded HS / PCs.

An assay for autologous primary immune cells is described, in which individual blood cells can be functionally screened simultaneously for individual antigens of interest, such as T-cell epitopes, without the need for HLA haplotype-specific reagents. An oligonucleotide-labeled hash-tracking system, followed by deconvolution via single-cell sequencing, correlates antigen reactivity with individual T cells.

The utility model discloses a trace automatic oscillation blending device which comprises a guide sleeve, a sleeve bottom cover detachably arranged in the middle of the lower end of the guide sleeve, a reagent tube supporting table arranged in the middle of the inner wall of the guide sleeve in a sliding mode, and a reagent tube lantern ring arranged over the reagent tube supporting table. A trace reagent tube is arranged in the middle of the reagent tube lantern ring, and the self-adaptive variable-diameter calibration mechanism is arranged in the middle of the reagent tube lantern ring and is used for centering and positioning the trace reagent tube; the eccentric reciprocating lifting mechanism is arranged at the bottom of the reagent tube supporting table and is used for vibrating the trace reagent tubes. By arranging the eccentric reciprocating lifting mechanism and utilizing the intermittent transmission mode of the inclined eccentric wheel and the linkage lock teeth, the reagent tube supporting table and the trace reagent tube can be driven to do reciprocating lifting motion along the guide sleeve, it is guaranteed that plasma and blood cells form the turbulent flow uniform mixing effect in the vertical direction, and the problem that existing layered interfaces are not sufficient in uniform mixing is solved.

The invention discloses a hematology analyzer and a detection method for hole blockage of a flow chamber, in the hematology analyzer provided by the invention, in the process that sample liquid enters the flow chamber for sample detection, a control unit can also obtain signal parameters based on optical signals of the sample liquid flowing through the flow chamber; whether the flow chamber is blocked or not is judged, the safety of the flow chamber is improved, and the detection efficiency of an optical detection system on sample liquid is improved. Meanwhile, in the process of detecting the sample liquid, whether the flow chamber has the hole blocking problem or not can be judged, the step of judging the hole blocking of the flow chamber does not need to be additionally set, the operation efficiency of the hematology analyzer is improved, and the use experience of a user on the hematology analyzer is improved.

The invention relates to the technical field of pickling of meat products, and discloses an internal and external coloring pickling process of meat products, which comprises the following steps: firstly collecting blood of a meat product to be pickled, separating blood cells from plasma by adopting a centrifugal separation method, and then carrying out hemolysis treatment on the blood cells by utilizing an acidic buffer solution. According to the internal and external coloring and pickling process of the meat product, processing equipment, a natural coloring agent, a natural preservative and a natural pickling agent are adopted, and when hemoglobin pigment is extracted, centrifugal separation and ion exchange chromatography technologies are applied, so that high purity of the pigment is ensured, and a basis is provided for stable internal coloring. In the rolling and pickling link, composite natural microorganism fermentation pickling liquid is used, components of the composite natural microorganism fermentation pickling liquid are carefully screened and cultured, microorganisms such as lactic acid bacteria and the like and metabolite antibacterial polypeptides thereof not only can inhibit growth and reproduction of harmful microorganisms, but also can enable meat products to uniformly absorb components such as salt and spices in the pickling process, so that the meat products are not prone to damage. And the quality difference caused by non-uniform pickling is effectively avoided.

A system includes: a dialysis system configured to provide a dialysis treatment for a patient; an optical monitoring system in communication with the dialysis system, wherein the optical monitoring system is configured to obtain measurements of the patient's hematocrit, oxygen saturation and / or blood volume change during the dialysis treatment, wherein the measurements are indicative of a double pool refill rate for the patient during the dialysis treatment; and a massaging system in communication with the dialysis system, wherein the massaging system is configured to provide massaging for the patient during the dialysis treatment, wherein providing massaging for the patient includes running a massage sequence taking into account the measurements obtained by the optical monitoring system.

1. Name of the product in this design: Graphical User Interface for Blood Sample Analysis of Electronic Device. 2. Purpose of this design: An electronic device. 3. The key design feature of this product is its graphical user interface. 4. The image or photograph that best illustrates the design's key features: the front view. 5. The product carrier uses a conventional design, omitting the rear view, left view, right view, top view, and bottom view. 6. Uses of the graphical user interface: for data analysis functions such as blood cell counting, white blood cell differential, and hemoglobin concentration measurement. 7. Human-computer interaction method of graphical user interface: The main view is the blood sample information and parameter results interface. Clicking "Start Test" on this interface will enter the change state diagram 1 to display patient information. Clicking "Next" on this interface will enter the change state diagram 2 to display test items. Clicking "Next" on this interface will enter the change state diagram 3 to display test status. 8. Other situations requiring explanation: In the change state diagram 1, the light yellow covered area represents the content screen, and the design for which protection is sought does not include color.

The application discloses a red blood cell room temperature storage solution and a preparation method and application thereof, and belongs to the technical field of biological medicines. The red blood cell storage agent comprises the following components in parts by weight: 1-200 parts of an anticoagulant, 1-200 parts of a blood cell stabilizer, 1-100 parts of a metabolism inhibitor, 1-100 parts of an electrolyte, 1-150 parts of an antioxidant, 350-1000 parts of deionized water, and pH 4.6-7.0. The components in the storage agent cooperate with each other and have a synergistic effect, and the multi-component synergistic protection can keep the red blood cell membrane integrity; the electrolyte balance osmotic pressure is maintained, the antioxidant removes free radicals and reduces hemolysis; the storage time is prolonged, the cell morphology and function are good; the cold chain dependence is reduced, and the demand for deep low-temperature equipment is reduced.

The application provides a reprogramming vector for blood cells and application thereof. Through the technical scheme in the disclosure, a low-cost and high-benefit solution can be provided for efficient reprogramming and application of iPSCs, and the wide application of iPSCs in clinical and scientific research fields is significantly promoted, and the iPSCs have important application value and development potential in research and practice in aspects such as disease models, drug screening, cell therapy and the like.

The invention belongs to the technical field of interpretable active learning, and particularly relates to a cell image attribute collaborative driven interpretable active learning method, which comprises the following steps of: in an inspection process, acquiring an image from a blood cell smear by using a camera, constructing a newly acquired cell image data set D1, randomly extracting a small amount of samples from the data set D1 to construct an initial labeling set D2; performing category labeling on the D2 and completing model pre-training based on the D2, performing standardization and preprocessing on the remaining unlabeled data in the D1, dividing the remaining unlabeled data into a plurality of batches according to the number to form a candidate unlabeled pool D3, sequentially inputting the data in the D3 into a model according to a preset batch, and for each batch of input data, adopting a class imbalance adaptive sample screening mechanism CASS to obtain a candidate unlabeled pool D3; the method comprises the following steps: comprehensively utilizing three types of indexes of uncertainty, representativeness and diversity under current weight configuration, introducing attribute information, carrying out weighted calculation in combination with a type frequency and an effective sample number, and screening to obtain candidate high-value samples of the batch;

The present disclosure relates to a method for white blood cell differential counting using whole blood in a fluid state, and the like. The method of the present disclosure does not include the drying and washing processes used in traditional blood cell analysis to provide a standard for multiple white blood cell analysis, thereby enabling rapid analysis with high repeatability and reproducibility even when large quantities of samples are being processed, and providing accurate test results including the numbers, ratios, and morphological discrimination for each type of white blood cells.

The invention discloses a microscopic image small target detection method and system based on multidirectional gating convolution, and the method comprises the steps: firstly obtaining a microscopic image data set, and carrying out the preprocessing; and secondly, inputting the preprocessed microscopic image into a target detection model YOLOv8n, and performing target detection to obtain a target detection result. And finally, training the target detection model YOLOv8n by adopting an end-to-end training normal form of single-stage detection, and testing and evaluating after training. According to the invention, positioning jitter is reduced in a mild defocusing and anisotropic distortion scene, the detection stability of a slender target is improved, and the method can be stably applied to detection and counting tasks of micro objects such as bacteria and blood cells.

The invention discloses a POCT (Point of Care Testing) hematology analyzer. In the application, the first bearing position is arranged on the sample injection module to bear the detection card for receiving the first to-be-detected sample, and the second bearing position is arranged on the first detection module to bear the detection card after receiving the first to-be-detected sample, so that the receiving and detection of the first to-be-detected sample are separately carried out at different bearing positions of different modules; the transfer module is arranged at the output end of the driving module of the preprocessing module, so that the driving module can drive the transfer module to transfer the detection card from the first bearing position to the second bearing position after driving the pipetting consumable through the loading module to transfer the first to-be-detected sample into the detection card at the first bearing position; therefore, the driving module is ingeniously used for driving the transfer module, and a driving device special for driving the transfer module is avoided, so that the overall structure of the analyzer is simpler, the production cost is reduced, and the analyzer is more compact and miniaturized in structure.

The application provides application of microgel particles taking cationic monomers as raw materials in preparation of blood crosslinking gel materials, and application of the microgel particles in preparation of interfacial stable hemostatic materials. The hemostatic gel prepared from the microgel particles has a sea-island belt structure based on blood cell bridging, and meanwhile, the strength and toughness of the material are improved. The gel can stably adhere to and support a wound, thereby achieving a dynamic hemostatic effect. The application fully utilizes the characteristics of large bleeding amount in severe bleeding, activates and anchors blood cells through an exogenous pathway based on electrostatic action. The application overcomes weakening effect of blood on a hemostatic material hemostatic interface, promotes formation of a blood enhanced phase, meets mechanical strength required for hemostasis at a severe bleeding site, and meets mechanical stability during patient transfer. The material used in the application has good biocompatibility after dialysis, has low toxicity and side effects on the human body, and provides great convenience for emergency hemostasis and the like.

The application discloses a kind of based on DNA methylation's construction method of biological age prediction model, the application is downloaded from GEO data website, derived from Chinese population, contains calendar age data, whole blood sample 450k methylation chip data, by the method of elastic network combined bootstrap, 31 methylation sites of modeling candidate are screened, multiple linear regression, support vector machine, random forest and gradient boosting regression tree are used to carry out preliminary construction and evaluation of model, then further filter methylation sites using full subset regression, obtain methylation age prediction model based on 18 methylation sites.Finally, using any provincial team natural population data, methylation age prediction model is optimized, and finally the biological age prediction model based on 18 methylation sites is obtained.The model is suitable for Chinese population, the number of methylation sites contained is less, is not affected by blood cell components, and the prediction accuracy is good.

The utility model discloses a blood cell smear auxiliary device. The blood cell smear auxiliary device comprises a blood filling pool and an adjusting part, the blood filling pool is provided with a containing groove used for containing blood, the groove bottom face of the containing groove is opened to be used for being matched with a glass slide, and the adjusting part comprises a first adjusting piece and a second adjusting piece. The first adjusting piece and the second adjusting piece are oppositely arranged in the blood filling pool, and the first adjusting piece and the second adjusting piece can move so as to adjust the volume of the containing groove. According to the blood cell smear auxiliary device, the preparation efficiency and quality of the blood smear can be remarkably improved, and a more reliable sample is provided for hematological examination, so that the accuracy and efficiency of medical diagnosis are promoted.

This application provides a method for using a POCT hematology analyzer, comprising: in response to a reagent kit being placed in a test chamber, controlling a pipette to move above the first mounting head reservoir of the reagent kit and descending to load the first mounting head; controlling the pipette to move to the diluent reservoir of the reagent kit to transfer the diluent into the pre-cell for impedance detection; controlling the pipette to transfer a sample into the pre-cell; mixing the test solution in the pre-cell; controlling the metal shielding cap to close with the test chamber to establish a connection between the rear cell and the pressure building system of the reagent kit; controlling the pressure building system to guide the test solution from the pre-cell into the rear cell; and detecting the test solution by impedance detection.

The invention discloses a blood cell separation magnet device and method, and belongs to the technical field of cell separation. The magnet device comprises a plurality of pole heads which are symmetrically arranged, and runway coils are arranged outside the pole heads; the central axis position of a gap defined by the runway coils is a working area, the working area is a uniform magnetic field capturing area, and magnetized target cells in blood cells are stably separated by using a uniform magnetic field; the width of the working area ranges from 8 mm to 15 mm, and the length of the working area ranges from 200 mm to 500 mm. Through cooperation of the runway coil and the pole head, a linear or planar working area which is small in space body, high in strength, large in gradient and uniform in magnetic field distribution is formed, and compared with an existing point-shaped or small-area high gradient, magnetized cells flowing through a sample in the area can be captured more efficiently and more thoroughly, and the recovery rate and the purity are remarkably improved.