966 results about "Blood cell" patented technology

The invention relates to the technical field of target detection, and discloses a blood cell detection method based on improved YOLOv8. According to the method, a convolutional attention module (CBAM) is introduced, so that the detection performance of the model on a shielded small target is improved; a new loss function S-MPDIOU is set, so that the accuracy and robustness of the loss function are further improved; the multi-scale feature fusion technology is adopted to better capture blood cell information of different sizes, so that the model can more accurately identify small target platelets; the recall rate is improved by improving a non-maximum suppression (NMS) algorithm, and particularly under the condition of dense blood cells or complex forms, the improved NMS algorithm can more effectively solve the overlapping problem and reduce false detection and missing detection. Through testing, the model can accurately identify and position different types of blood cells such as red blood cells, white blood cells and blood platelets in an image, and a new effective method is provided for blood cell detection.

The present application provides a test kit and a POCT blood cell analyzer. The test kit includes a box body, a sealing ring, a microporous sheet, and a back pool body. The box body includes a front pool and a mounting cavity connected to the front pool; the sealing ring is arranged in the mounting cavity and is located on the side of the mounting cavity close to the front pool; the microporous sheet is provided with micropores that allow cells to pass through one by one; the microporous sheet is arranged in the mounting cavity and is located on the side of the sealing ring away from the front pool; the back pool body abuts against the side of the microporous sheet away from the sealing ring, and is used to fix the microporous sheet and the sealing ring on the box body; the back pool body is formed with a drainage cavity, and the front pool and the drainage cavity are connected through micropores. The test kit provided by the present application has a simple structure, is easy to assemble, and is conducive to the processing of the test kit.

Methods and devices for analyzing a sample from a subject are provided. A device includes one or more coherent light sources and one or more partially coherent light sources. The light sources are configured to emit first and second wavelengths of electromagnetic radiation towards an imaging chamber configured to hold blood cells. The light sources are aligned to illuminate a single common area of the imaging chamber. The device includes optics positioned to receive light from the imaging chamber, and a detector in optical communication with the optics. The detector is configured to detect fluorescence emission and backscatter from blood cells when present in the imaging chamber. The device includes mixers / unmixers configured to replicate and mix first and second signals representative of the responses to the first and second wavelengths after interacting with the plurality of blood cells to generate spectro-spatial responses.

The invention provides a blood cell image recognition method based on a multi-scale Vision Transform model, and the method comprises the steps: constructing a multi-scale feature extraction architecture, combining the global modeling capability of a Transform network, carrying out the parallel processing of Patches of different sizes, and fusing the multi-scale information, thereby achieving the recognition of a blood cell image. The cells in the blood smear can be comprehensively perceived from microscopic details to macroscopic contexts, so that not only is the adaptability of the model to cell size difference and morphological diversity enhanced, but also the recognition stability under the conditions of cell overlapping and dyeing difference in a complex visual field is improved; the accuracy and calculation efficiency of cell classification and counting are effectively improved, and full-process automation from visual field quality evaluation to automatic cell recognition and counting is achieved.

Disclosed are methods of treating a subject having clinically significant signs and symptoms associated with blood cell differentiation and treated with an agent that reduces the level and / or activity of BRG1 and / or BRM. The methods disclosed herein may include, e.g., administering to the subject an effective amount of a corticosteroid, hydroxyurea, or furosemide, or subjecting the subject to leukapheresis.

Disclosed are methods, means, and compositions of matter useful for the stimulation of angiogenesis directly by administration of membrane vesicles, such as fibroblast-derived exosomes, and / or through induction of angiogenic cytokines from blood cells contacted with fibroblast-derived exosomes. The invention provides means of treating conditions in which angiogenesis is beneficial through local or systemic administration of exosomes, including those derived from fibroblasts, wherein the fibroblasts are cultured under basal conditions or conditions of hypoxia. In other embodiments exosomes derived from fibroblasts are utilized to augment endogenous regenerative processes, such as hematopoiesis, angiogenesis and neurogenesis, as well as augment regenerative processes stimulated by administration of exogenous therapeutics such as cells, growth factors, or genes.

The present disclosure relates to compositions and methods of increasing levels of fetal hemoglobin (HbF) in cells. The present disclosure further relates to methods for treating patients suffering from blood cell diseases, including those associated with reduced amounts of functional adult hemoglobin (HbA), such as sickle cell disease and β-thalassemias

The invention provides a blood smear device and method for a clinical laboratory. The method comprises the following steps: collecting a staining image of a blood smear; segmenting a gathering region and a sparse region of the blood cells in the blood smear from the dyed image, and determining edge features of each blood cell in the dyed image; performing overlapping analysis on the overlapped blood cells in the aggregation area based on the edge features of the blood cells in the aggregation area to obtain an overlapping influence area of the blood cells in the aggregation area; based on the edge features of the blood cells in the sparse region, reconstructing gaps among the blood cells in the sparse region to obtain a gap reconstruction region of the blood cells in the sparse region; determining the density distribution characteristics of the blood cells in the dyeing image according to the overlapping influence area and the gap reconstruction area; and detecting the uniformity of the blood smear according to the density distribution characteristics. By adopting the scheme of the invention, the blood cell distribution condition can be accurately identified under complex interference factors.

In some embodiments, a method for classifying elements of a blood sample is provided, the method including: digitally staining an image of the blood sample using a trained machine learning model to generate a digitally stained image; extracting one or more intermediate features generated by the trained machine learning model during the digital staining of the image; providing the one or more extracted intermediate features to a trained multi-class classifier; and employing the trained multi-class classifier to classify at least one element in the blood sample based on the one or more extracted intermediate features. Many other embodiments are also provided.

A blood cell analyzer (100) and a blood cell analysis method (300). The blood cell analysis method (300) comprises: sucking a blood sample to be tested; preparing a first determination sample and obtaining first optical information; preparing a second determination sample and obtaining second optical information; generating a first leukocyte classification scatter diagram and a second leukocyte classification scatter diagram; determining a first counting result of neutrophils of the to-be-detected blood sample based on the first leukocyte classification scatter diagram and the second leukocyte classification scatter diagram; determining an actual distribution area of neutrophils from the first leukocyte classification scatter diagram; based on a reference distribution region of neutrophils of a reference leukocyte classification scatter diagram of the normal blood sample, determining a leaf-division nucleophore distribution region from the actual distribution region; determining a second counting result of the cells falling into the leaf-separating nucleogranulocyte distribution area; and based on the first counting result and the second counting result, determining a counting result of the rod-shaped nuclear granulocytes in the to-be-detected blood sample.

The invention discloses a portable blood cell real-time classified counting system and method based on a big data algorithm, and relates to the technical field of biomedical engineering. Aiming at the problems of insufficient portability, poor real-time performance and difficulty in complex cell feature recognition of traditional blood cell classification counting equipment, the system realizes fusion acquisition of high-resolution cell images and dynamic spectral information through a multi-modal image acquisition module; according to the system, intelligent preprocessing and multi-scale feature extraction of cell images are completed by using a self-adaptive preprocessing unit and a lightweight feature extraction engine; according to the system, cell classification algorithm optimization based on meta-learning and clinical knowledge is realized by means of a big data enhancement classifier; according to the system, an intelligent interaction terminal and an ultra-low power consumption hardware architecture are combined, so that real-time visualization of a cell classification result and miniaturization integration of equipment are realized; and finally, in a portable scene, the accuracy, the real-time performance and the equipment usability of blood cell classification counting are remarkably improved.

The invention discloses an anti-lipopolysaccharide factor rALF-like protein and application thereof, and belongs to the technical field of molecular biology. The anti-lipopolysaccharide factor rALF-like protein provided by the invention belongs to secretory protein, the rALF-like protein can be competitively combined with an LPS structural domain of bacteria to achieve the purpose of inhibiting inflammation, and the expression of the coding gene Pv-ALF-like gene in blood cells, hepatopancreas, gill and intestinal tissues is remarkably up-regulated, so that the anti-lipopolysaccharide factor rALF-like protein can be used for inhibiting inflammation. The rALF-like protein disclosed by the invention has antibacterial and antiviral effects, lays a foundation for developing antibacterial and antiviral drugs and functional feed additives, and plays an important role in improving the resistance link of prawns.

The invention relates to the field of plasma separation and detection, in particular to a composition for separating plasma and a using method thereof. The composition comprises: a phase change component, which is selected from n-alkanes, which are selected from at least one of n-tetradecane, n-pentadecane, n-hexadecane, n-heptadecane, n-octadecane, n-nonadecane, n-eicosane, n-heneicosane or n-docosane; a liquid phase carrier component, wherein the liquid phase carrier component is selected from silicone oil; the structural framework and rheology regulation and control component comprises fumed silica, and the structural framework and rheology regulation and control component comprises fumed silica; the dispersion and stabilization auxiliary agent component is selected from at least one of TiO2 and BaSO4, the density adjusting filler component is selected from at least one of TiO2 and BaSO4, and the dispersion and stabilization auxiliary agent component comprises a silane coupling agent. According to the device, plasma and blood cells can be reliably, rapidly and effectively separated only through one-time low-temperature centrifugation, and remixing caused by transportation jolting can be avoided.

The current application reveals a method, apparatus, device, and storage medium for detecting micro residual lesions, falling within the domain of medical detection technology. This method is based on differentiated deep whole-exome / targeted drug sequencing and tissue-blood cell-plasma co-capture technology, and 100,000× ultra-high depth personalized / high evidence hotspot combination panel sequencing to evaluate tiny residual lesions and tumor evolution / second primary in plasma samples. It resolves the challenges of existing techniques, such as elevated tissue detection thresholds, restricted tracking locations, inadequate detection sensitivity and precision, or elevated costs when ctDNA concentrations in the bloodstream are minimal. Furthermore, it surmounts the challenge of simultaneously achieving personalized tracking detection and monitoring tumor evolution or second / primary detection. It markedly boosts the precision of forecasting the likelihood of recurrence following patient therapy within a restricted budget.

The utility model discloses a portable peripheral blood cell counter which comprises a glass slide and a cover glass, and the cover glass is positioned at the upper end of the glass slide; the carrying and placing frame is arranged at the lower end of the glass slide, a rectangular placing open groove is formed in the upper end face of the carrying and placing frame, L-shaped positioning pieces are fixed to the four corners of the upper end of the rectangular placing open groove in the carrying and placing frame, and the glass slide is located in the rectangular placing open groove and clamped among the four L-shaped positioning pieces; the semicircular through groove is formed in the middle of the front end face and the rear end face of the carrying and placing frame, and the semicircular through groove vertically penetrates through the carrying and placing frame. According to the blood cell counter, external protection of glass slides and cover glass is achieved, the effect that each group of cover glass and glass slides are separated is achieved, and therefore the problems that a main body of an existing blood cell counter used for counting is made of glass slides and cover glass, the glass slides and the cover glass are prone to colliding with each other during placement, damage can occur, and peripheral blood cell counting is affected are effectively solved.

The invention relates to the technical field of blood cell detection, and provides an intelligent blood cell detection device and a blood cell image splicing method.The intelligent blood cell detection device comprises a device shell, a base, a micro-motion positioning assembly, an imaging support, an observation assembly, a placement table and a two-way positioning assembly, the base is fixedly installed in the device shell through the damping foot pad, the micro-motion position adjusting assembly is installed on the base, the imaging support is installed on the micro-motion position adjusting assembly, the observation assembly is installed on the imaging support, the placing table is arranged on the base, and the bidirectional position adjusting assembly is installed on the base. According to the technical scheme, the problems that in the prior art, when a specimen is subtly observed, the observation position is difficult to accurately adjust in a small distance, adjacent images are difficult to obtain, and meanwhile the images are difficult to accurately splice are solved.

The invention relates to the technical field of blood cell analysis, and particularly discloses a blood cell analysis system and method based on computer vision, which uses an image recognition and generation technology based on computer vision to carry out grid division and semantic feature extraction on blood image data. Performing global text generation and semantic coding on each blood image data grid block feature after semantic extraction so as to perform adjustment response aggregation on attention of different blood local areas in each blood image data grid block feature according to blood image text description semantic features; therefore, intelligent optimization of the blood image data is realized. In this way, the complex deep-layer mutual influence association relationship between cells can be captured, so that a more accurate diagnosis basis is provided. Meanwhile, the processing strategy can be dynamically adjusted according to the specific description content of the blood image so as to better adapt to diversified samples, and the method does not only depend on fixed preprocessing parameters.

The present invention relates to methods and products for assessing autophagic flux, and more specifically provides a biochemical functional measure used to quantify autophagic flux in sub-populations of blood cells. The present invention also provides lysosomal system markers for use in the methods described herein, systems for assessing autophagic flux, methods of identifying markers indicative of autophagic flux, and methods of treating subjects found to be suffering from, or susceptible to, a disease, condition or state associated with autophagic flux dysfunction using the methods described herein.

Fluid analyzation devices, methods, and systems are disclosed including an analyzation device comprising a sample vessel having an outer surface, a microchannel within the confines of the outer surface, a first port extending through the outer surface to the microchannel, and a second port extending through the outer surface to the microchannel; and an piezo transducer bonded to the outer surface of the sample vessel to form a monolithic structure, the piezo transducer configured to emit ultrasonic acoustic waves having a first frequency, a second frequency, and a third frequency into and / or to a blood sample within the microchannel, the first frequency configured to begin separation of red blood cells and plasma in the blood sample, the second frequency configured to complete separation of the red blood cells and plasma, and the third frequency configured to rupture cell walls of the blood cells producing a lysed blood sample.

The invention provides a precise magnetic control whole blood rare cell flow magnetic separation system, a magnetic separation and identification method and application, and belongs to the technical field of micro-control flow biological detection. According to the invention, the magnetic separation chip can form a gradient magnetic field, and cells are fixed at a certain position on the sealing film under the combined action of magnetic field force and friction force, so that the separation of the cells is realized. Whole blood cell detection is carried out based on the gradient magnetic field, whole blood sample loading treatment can be directly carried out, the problem that the capture rate is reduced due to mechanical death, damage and stress gene expression caused by repeated elution / transfer steps is avoided, and the capture efficiency and cell activity of the chip are guaranteed; the magnetic field provided by electricity is not needed, the cost is low, the detection accuracy is high, the problems existing in existing CTC detection are solved, economic and practical values are achieved, further development of CTC detection can be promoted, and a novel and effective method is provided for complete blood cell separation detection.

The invention relates to a specially selected strain and application thereof in cardiovascular and cerebrovascular maintenance. The invention provides a specially selected strain-prebiotic composition for enhancing and maintaining an ozone high-dose blood purification effect and application of the specially selected strain-prebiotic composition in establishment of an intestinal barrier function and health maintenance of heart and cerebral vessels. According to the invention, blood cell metabolism, immune system and antioxidant enzyme system can be activated by autologous blood circulation reinfusion, the state of the blood vessel wall is improved, and the physiological function of blood cells is enhanced; the specially selected targeted strain-prebiotic composition can regulate intestinal flora balance, establish an intestinal barrier function, maintain and accelerate metabolism of a human body, decompose blood fat and toxin garbage accumulated in the body, promote detoxification of the intestinal tract, purify the intestinal tract and reduce intestinal toxin substances entering the blood circulation of the human body through a portal system. According to the combination scheme, the blood purification effect can be remarkably improved, the blood fat index is remarkably improved, the purposes of recovering the blood vessel function and enhancing the human immune function are jointly achieved, a new technical scheme and means are provided for prevention and treatment of cardiovascular and cerebrovascular diseases, and wide application prospects are achieved.

The invention relates to the technical field of intraoperative intelligent monitoring, and particularly discloses a spinal canal debonding method and system for real-time state evaluation, and the method comprises the steps: collecting neural structure microcirculation blood flow data in real time through integrating a micro-sensing unit at a working end part of a surgical instrument, and extracting blood flow oscillation frequency and capillary hematocrit parameters; performing multi-scale signal decomposition on the blood flow oscillation frequency to obtain an energy distribution parameter, and performing dynamic mode recognition on the hematocrit data to obtain a main variation characteristic value; fusing the two characteristic parameters to generate a nervous tissue vitality index; inputting the index into a pre-trained neural network for time sequence feature analysis, and outputting a graded ischemia risk early warning signal; the operation rhythm is automatically adjusted through a decision tree control mechanism based on the early warning signal, and self-adaptive regulation and control according to the state of the nervous tissue are achieved; the technical problem that the risk of neural ischemia cannot be monitored in real time in a traditional spinal canal release operation is solved, and occult nerve injury caused by asymptomatic neural ischemia can be prevented.

According to one embodiment, an initialization agent is for initializing blood cells derived from peripheral blood. The initialization agent includes an initialization factor group for producing iPS cells by initializing a group of blood cells including mononuclear cells derived from peripheral blood, and a group of lipid nanoparticles that encapsulate the initialization factor group. The lipid nanoparticles have a component of at least or more of 40% FFT-10 and FFT-20. FFT-10 and FFT-20 are included in equal amounts.

The present invention relates to systems and methods for optimizing plasma collection volume. A plasma withdrawal system and a method for operating a plasma withdrawal system are provided by which the volume / weight of the anticoagulated plasma collected is optimized. In one example, a nomogram is provided that utilizes the donor hematocrit to calculate the volume / weight of raw plasma within a plasma product having a maximum volume allowed by the FDA nomogram. In a plasma withdrawal process having multiple collection phases followed by a reinfusion cycle in which concentrated red blood cells are returned to the donor, the volume of plasma product to be collected is calculated prior to the start of each collection cycle to account for the increasing hematocrit of the donor, resulting in a greater total volume of plasma product to be collected during the plasma withdrawal process.

The invention discloses a method for preparing an amino acid water-soluble fertilizer by processing animal blood, which belongs to the technical field of water-soluble fertilizer preparation, and comprises the following steps: step 1, collecting animal blood, and performing centrifugal treatment to remove part of impurities and blood cell fragments in the blood to obtain a relatively pure plasma part; 2, compound protease is added into the pretreated animal blood for an enzymolysis reaction, meanwhile, sodium selenite is added into an enzymolysis system, and the sodium selenite serves as a selenium source; 3, after enzymolysis is completed, a compound microbial agent is added into enzymatic hydrolysate, and aerobic fermentation is carried out; according to the method for preparing the amino acid water-soluble fertilizer through animal blood processing, the structural design is reasonable, the added selenium element can enhance the stress resistance of plants and improve the quality of agricultural products, and an acidolysis method or an enzymolysis method and a liquid fertilizer or solid fertilizer production mode are flexibly selected, so that the optimal economic benefits and social benefits are achieved.

There is disclosed a vital sign measuring device and method for obtaining an indication of a person's blood pressure via the person's finger by means of an inflatable measurement bladder adapted to contact only a portion of an underside of a finger defining a finger pad. A structure or other implement applies a downward force on a top portion of the finger opposite the finger pad. Blood pressure may be determined without the use of any electromagnetic radiation, such as miniature dynamic light sensing (mDLS). Apparatuses and methods for obtaining an indication of other vital signs, including hematocrit and total protein, are also disclosed.

Methods and devices for analyzing a sample from a subject are provided. A device includes one or more coherent light sources and one or more partially coherent light sources. The light sources are configured to emit first and second wavelengths of electromagnetic radiation towards an imaging chamber configured to hold blood cells. The light sources are aligned to illuminate a single common area of the imaging chamber. The device includes optics positioned to receive light from the imaging chamber, and a detector in optical communication with the optics. The detector is configured to detect fluorescence emission and backscatter from blood cells when present in the imaging chamber. The device includes mixers / unmixers configured to replicate and mix first and second signals representative of the responses to the first and second wavelengths after interacting with the plurality of blood cells to generate spectro-spatial responses.

The invention relates to an antibody composition for detecting abnormal CTL (cytotoxic T lymphocyte) cells of hemophagocytic syndrome and application of the antibody composition, and the antibody composition comprises a CD2 antibody, a CD3 antibody, a CD4 antibody, a CD5 antibody, a CD7 antibody, a CD8 antibody, a CD56 antibody, a CD57 antibody, a Granzyme B antibody, a Perforin antibody, a TRBC1 antibody and a CD45 antibody. In the technical scheme provided by the invention, key phenotypic characteristics of CTL (Cytotoxic Lymphocyte) can be completely covered by various antibodies aiming at T cell and NK cell surface markers, T large granular lymphocyte markers, cytotoxic granular proteins, T cell receptor constant regions and leukocyte common antigens; wherein the clonality of the CTL cells is judged through the expression level of the TRBC1 antibody, and the cytotoxic function activity of the CTL cells is evaluated through the expression levels of Granzyme B and Perforin.

The present disclosure provides a method for obtaining a cancer risk prediction marker, a cancer risk assessment method, an electronic device, and a storage medium performed by a machine. The method for obtaining a cancer risk prediction marker includes: analyzing a plurality of peripheral blood circulating cell-free DNA (cfDNA) fragments of a biological sample of a subject to obtain sequence reads; obtaining data of a distribution of a nucleosome, and determining a predetermined interval upstream and downstream of a nucleosome center position as a nucleosome protection region; screening sequence reads of partial cfDNA fragments whose ends are located in the nucleosome protection region from the sequence reads of the plurality of cfDNA fragments; calculating a first fragmentation feature in the sequence reads of the plurality of cfDNA fragments, and calculating a second fragmentation feature in the sequence reads of the screened partial cfDNA fragments; and calculating one or more difference values of a change level of the second fragmentation feature relative to the first fragmentation feature as a cancer risk prediction marker.

The invention relates to the technical field of medical instruments, provides a catheter assembly with flow regulation, backflow prevention and self-adaptive sealing functions, and particularly relates to a peripheral vessel catheter assembly for hemodialysis. The self-adaptive anti-backflow catheter mainly comprises a catheter body, a flow adjusting and anti-backflow linkage unit and a self-adaptive sealing unit. The independent cavities of the main channel and the auxiliary channel can avoid turbulent flow generated by mixing of high-flow blood and medicine in the single-cavity catheter, so that mechanical damage to blood cells is reduced, the flow adjusting and backflow preventing linkage unit accurately adjusts flow through a pressure induction type adjusting piece, a lever mechanism and simple mechanical transmission, and the flow adjusting effect is good. And a bistable silica gel membrane backflow blocking petal and an electromagnetic locking device are matched, so that backflow is reliably prevented. The hemodialysis device has excellent performance in the aspects of flow regulation, backflow prevention, sealing and self-cleaning, and the safety and effectiveness of hemodialysis are remarkably improved.