Test kits and POCT blood cell analyzers

Abstract

The present application provides a test kit and a POCT blood cell analyzer. The test kit includes a box body, a sealing ring, a microporous sheet, and a back pool body. The box body includes a front pool and a mounting cavity connected to the front pool; the sealing ring is arranged in the mounting cavity and is located on the side of the mounting cavity close to the front pool; the microporous sheet is provided with micropores that allow cells to pass through one by one; the microporous sheet is arranged in the mounting cavity and is located on the side of the sealing ring away from the front pool; the back pool body abuts against the side of the microporous sheet away from the sealing ring, and is used to fix the microporous sheet and the sealing ring on the box body; the back pool body is formed with a drainage cavity, and the front pool and the drainage cavity are connected through micropores. The test kit provided by the present application has a simple structure, is easy to assemble, and is conducive to the processing of the test kit.

Description

Technical Field

[0001] The present application relates to the field of medical device technology, and in particular to a test kit and a POCT blood cell analyzer. Background Art

[0002] Hematology analyzers are commonly used medical testing devices that measure parameters such as the number and proportion of blood cells (red blood cells, white blood cells, and platelets). Through blood analysis, they can identify microbial infections, diagnose and treat anemia, and diagnose blood disorders. With advancements in technology and scientific research, hematology analyzers have seen their functions expand, performance improve, and automation levels increase, leading to widespread clinical application.

[0003] However, the installation of the microporous sheet in the existing POCT blood cell analyzer is relatively complicated and there may be a risk of leakage. In order to ensure its sealing and convenient installation, the installation structure of the existing microporous sheet needs to be optimized. Summary of the Invention

[0004] The present application provides a test kit and a POCT blood cell analyzer to solve the technical problems of the prior art in that the installation structure of the microporous sheet is complex and the sealing performance is poor.

[0005] In order to solve the above technical problems, a technical solution adopted in the present application is: to provide a test kit, which includes: a box body, including a front pool and an installation cavity connected to the front pool; a sealing ring, arranged in the installation cavity, and located on the side of the installation cavity close to the front pool, a microporous sheet, provided with micropores allowing cells to pass through one by one, the microporous sheet is arranged in the installation cavity, and located on the side of the sealing ring away from the front pool, a rear pool body, the rear pool body abuts against the side of the microporous sheet away from the sealing ring, and is used to fix the microporous sheet and the sealing ring on the box body, the rear pool body is formed with a drainage cavity, and the front pool and the drainage cavity are connected through the micropores.

[0006] Furthermore, the box body includes a through hole connecting the front pool and the installation cavity, the ratio of the diameter of the microporous sheet to the diameter of the through hole is not less than 1.7, and the diameter of the microporous sheet is not greater than the inner diameter of the installation cavity.

[0007] Furthermore, the sealing ring is clearance-matched with the installation cavity.

[0008] Furthermore, the rear pool body is bonded to the box body, and the microporous sheet and the sealing ring are bonded to the box body. Alternatively, the rear pool body is bonded to the box body, and the microporous sheet and the sealing ring are bonded to the rear pool body.

[0009] Furthermore, a positioning protrusion is provided on the outer end surface of the installation cavity, and a positioning groove is provided on the rear pool body, and the positioning protrusion cooperates with the positioning groove to position the rear pool body, or a positioning groove is provided on the outer end surface of the installation cavity, and a positioning protrusion is provided on the rear pool body, and the positioning protrusion cooperates with the positioning groove to position the rear pool body.

[0010] Furthermore, the front cell is provided with a front cell electrode, and the rear cell body is provided with a rear cell electrode extending toward the drainage cavity. The front cell electrode and the rear cell electrode are spaced apart and located on both sides of the microporous sheet, and the inner end surface of the rear cell electrode is a convex surface.

[0011] Furthermore, the rear cell body includes a bottom wall and a side wall, the bottom wall is connected to the side wall to form a drainage cavity, the rear cell electrode is arranged on the bottom wall, and a liquid outlet is provided on the side wall, the liquid outlet is connected to the drainage cavity, the bottom wall is a plane, and the plane and the side wall are inclined. The liquid outlet is arranged at the intersection of the bottom wall and the side wall, and is located on the side away from the microporous sheet.

[0012] Furthermore, the rear cell body includes a bottom wall and side walls, the bottom wall is connected to the side walls to form a drainage cavity, the rear cell electrode is arranged on the bottom wall, and the distance from the bottom wall to the microporous sheet gradually decreases from the position where the rear cell electrode is arranged on the bottom wall to the edge of the bottom wall.

[0013] Furthermore, one side surface or both sides of the microporous sheet are provided with a concave guide surface around the micropores.

[0014] To solve the above technical problems, another technical solution adopted in this application is: to provide a POCT blood cell analyzer, which includes a test kit of any of the above embodiments and a detection seat that cooperates with the test kit, and the POCT blood cell analyzer is used for analyzing and detecting blood samples.

[0015] The beneficial effects of the present application are as follows: Unlike the prior art, the test kit of the present application includes a box body, a sealing ring, a microporous sheet, and a back cell body, wherein the sealing ring, microporous sheet, and back cell body are sequentially arranged in the mounting cavity of the box body, and the back cell body is used to fix the microporous sheet and sealing ring to the box body. The test kit of the present application has a simple structure and is easy to assemble. The novel fixing structure of the microporous sheet ensures better sealing of the front end of the mounting cavity, reduces the risk of leakage, and can improve the reliability of the test kit detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present application. Those skilled in the art can also derive other drawings based on these drawings without inventive work, among which:

[0017] Figure 1 Schematic diagram of the structure of one embodiment of the kit provided by the present application;

[0018] Figure 2 yes Figure 1 An exploded schematic diagram of the kit is shown;

[0019] Figure 3 yes Figure 1 A schematic cross-sectional view of the kit shown;

[0020] Figure 4 yes Figure 1 A schematic structural diagram of an embodiment of a rear cell body in the kit shown;

[0021] Figure 5 yes Figure 1 A schematic structural diagram of another embodiment of the rear cell body in the kit shown;

[0022] Figure 6 is a schematic structural diagram of another embodiment of the kit provided by the present application;

[0023] Figure 7 yes Figure 6 An exploded schematic diagram of the kit is shown;

[0024] Figure 8 is a schematic structural diagram of another embodiment of the kit provided by the present application;

[0025] Figure 9 yes Figure 8 An exploded schematic diagram of the kit is shown;

[0026] Figure 10 is a schematic structural diagram of another embodiment of the kit provided by the present application;

[0027] Figure 11 It is a structural schematic diagram of an embodiment of a pipette and a pipette tip in a POCT blood cell analyzer provided in the present application. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are only part of the embodiments of this application, not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0029] It should be noted that if the embodiments of the present application involve directional indications (such as up, down, left, right, front, back, etc.), the directional indications are only used to explain the relative position relationship, movement status, etc. between the various components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indications will also change accordingly.

[0030] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present application, the descriptions of "first", "second", etc. are only for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of such features. In addition, the technical solutions between the various embodiments can be combined with each other, but they must be based on the fact that they can be implemented by ordinary technicians in this field. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by this application.

[0031] In the first embodiment, the present application provides a kit, such as Figure 1-Figure 3 As shown, Figure 1 This is a schematic structural diagram of an embodiment of the kit provided by the present application. Figure 2 yes Figure 1 An exploded schematic diagram of the kit is shown; Figure 3 yes Figure 1 The cross-sectional structure diagram of the reagent kit shown in one perspective, the reagent kit includes: a box body 10, a sealing ring 20, a microporous sheet 30 and a back cell body 40.

[0032] Specifically, if Figure 1 and Figure 2 As shown, the box body 10 includes a forecell 101 and a mounting cavity 102 connected to the forecell 101. In this embodiment, the forecell 101 is an impedance detection cell and is provided with two groups, respectively for coordinating WBC (white blood cell) detection and RBC (red blood cell) detection. In other embodiments, the forecell 101 can also be provided with one or at least three groups, etc., for detecting red blood cells, white blood cells, or other items, and the specific configuration can be based on actual needs.

[0033] like Figure 3As shown, the sealing ring 20 is disposed in the mounting cavity 102 and is located on the side of the mounting cavity 102 close to the front cell 101. The microporous sheet 30 is provided with micropores 31 that allow cells to pass through one by one. The microporous sheet 30 is disposed on the side of the sealing ring 20 away from the front cell 101. The rear cell body 40 is located on the side of the microporous sheet 30 away from the sealing ring 20. The rear cell body 40 is used to fix the microporous sheet 30 and the sealing ring 20 on the box body 10. The rear cell body 40 and the mounting cavity 102 are snap-fitted, threaded, interference-fitted, laser-welded, or adhesively bonded.

[0034] In this embodiment, the sealing ring 20, the microporous sheet 30, and the rear cell body 40 are sequentially arranged in the installation cavity 102, and the microporous sheet 30 and the sealing ring 20 are pressed and fixed to the box body 10 by the rear cell body 40. This method ensures that the inner end of the installation cavity 102 is well sealed, improves the reliability of the test kit, and facilitates the processing and assembly of the test kit. Here, the inner end of the installation cavity 102 refers to the end of the installation cavity 102 near the front cell 101.

[0035] like Figure 3 As shown, the box body 10 includes a through hole 103 connecting the front pool 101 and the installation cavity 102. The sealing ring 20 is installed at the through hole 103 and is gap-fitted with the installation cavity 102. In this way, the sealing ring 20 will not tilt when it is placed in, which facilitates the installation of the sealing ring 20, and the liquid in the front pool 101 will not enter the rear pool body 40 from the edge of the sealing ring 20, thereby improving the reliability of the test kit detection.

[0036] Furthermore, the ratio of the diameter of the microporous sheet 30 to the diameter of the through-hole 103 is not less than 1.7, and the diameter of the microporous sheet 30 is not greater than the inner diameter of the mounting cavity 102. For example, the ratio of the diameter of the microporous sheet 30 to the diameter of the through-hole 103 is 1.7, 1.75, or 1.8, and the diameter of the microporous sheet 30 can be equal to or slightly smaller than the inner diameter of the mounting cavity 102. This approach facilitates the back cell body 40 to press the microporous sheet 30, prevents liquid from entering the back cell body 40 from the edge of the microporous sheet 30, and facilitates the installation of the microporous sheet 30, saving assembly time of the microporous sheet 30.

[0037] Optionally, the case body 10, sealing ring 20, microporous sheet 30, and rear cell body 40 are separate components. In one specific embodiment, the microporous sheet 30 and sealing ring 20 are bonded to the case body 10, and the rear cell body 40 is bonded to the case body 10. In another specific embodiment, the microporous sheet 30 and sealing ring 20 are bonded to the rear cell body 40, and the rear cell body 40 is bonded to the case body 10. This arrangement reduces assembly difficulty.

[0038] In other embodiments, the sealing ring 20, the microporous sheet 30 and the back cell body 40 may be an integrated structural component to reduce the number of assembly parts, lower the assembly difficulty and save assembly time.

[0039] The box body 10 and / or the back cell body 40 may be made of plastic. The microporous sheet 30 may be made of a plastic sheet or a ceramic sheet. Plastic sheets or ceramic sheets are relatively inexpensive and can be used as disposable products, eliminating the need for expensive materials that require repeated cleaning. The sealing ring 20 may be injection molded using a secondary injection molding process using a relatively soft plastic material.

[0040] Furthermore, if Figure 2 and Figure 3 As shown, the box body 10 is provided with a forecell electrode 60 corresponding to the forecell 101, and the rear cell body 40 is formed with a drainage cavity 41 (also referred to as the rear cell), and the forecell 101 and the drainage cavity 41 are connected through the micropore 31. The rear cell body 40 is provided with a rear cell electrode 50 extending into the drainage cavity 41, and the forecell electrode 60 and the rear cell electrode 50 are respectively spaced apart and located on both sides of the microporous sheet 30. The outer ends of the forecell electrode 60 and the rear cell electrode 50 (i.e., the two ends away from each other) are used to connect to the working voltage, and the inner ends of the forecell electrode 60 and the rear cell electrode 50 (i.e., the two ends close to each other) are in contact with the sample liquid to be tested. The liquid level of the sample liquid to be tested in the forecell 101 will be higher than the forecell electrode 60, and the drainage cavity 41 will be filled with the sample liquid to be tested during detection.

[0041] In the embodiment of the present application, the axis of the forecell electrode 60 and the axis of the rear cell electrode 50 are substantially aligned. Experimental verification has shown that detection accuracy is relatively high when the axis of the forecell electrode 60 and the rear cell electrode 50 are coaxial. In other embodiments, the axis of the forecell electrode 60 and the axis of the rear cell electrode 50 may not be aligned.

[0042] During the cell calculation process in the impedance channel, liquid in the forewell 101 passes through the microporous sheet 30, and the drainage cavity 41 (backwell) slowly fills with liquid. One or both surfaces of the microporous sheet 30 can be provided with concave limiting guide surfaces 32 around the micropores 31. Concave limiting guide surfaces 32 can be spherical or conical to guide the liquid in and out of the micropores 31.

[0043] Furthermore, the front cell electrode 60 and / or the back cell electrode 50 may be columnar electrodes. However, in this manner, the liquid column passing through the micropores 31 of the microporous sheet 30 may collide with the back cell electrode 50, causing backflow to impact the microporous sheet 30. This phenomenon, on the one hand, affects the stability of the signal, and on the other hand, may generate M waves, reducing the reliability of the detection.

[0044] To improve the above problem, in some embodiments, the inner end surface of the rear cell electrode 50 can be configured as a convex surface. For example, the inner end of the rear cell electrode 50 can be configured as a hemispherical surface, so that the inner end surface of the rear cell electrode 50 is a curved surface. In this way, the liquid backwash flow can be evenly dispersed from the center to the surrounding area, so that the backwash flow will not flow directly to the microporous sheet 30, thereby reducing the phenomenon of cell particle recoil. Wherein, the inner end surface refers to the end of the rear cell electrode 50 pointing to the fore cell 101 when the fore cell 101 is used as a reference, and the end away from the fore cell 101 is the outer end surface.

[0045] Furthermore, a liquid outlet 44 is provided on the rear pool body 40, the liquid outlet 44 is connected to the installation cavity 102, and the liquid outlet 44 is used to discharge the gas or liquid in the drainage cavity 41. The structure of the rear pool body 40 can also refer to the description of the second embodiment below, as shown in detail. Figure 4 and Figure 5 The description in the illustrated embodiment will not be repeated here.

[0046] The distance between the inner end surface of the rear cell electrode 50 and the microporous sheet 30 is not less than 5 mm. For example, the distance between the inner end surface of the rear cell electrode 50 and the microporous sheet 30 can be set to 5 mm, 6 mm, 7 mm, or 8 mm. By limiting the distance between the microporous sheet 30 and the inner end surface of the rear cell electrode 50, the microporous sheet 30 is prevented from being too close to the rear cell electrode 50, thereby providing sufficient buffer distance for the liquid, thereby facilitating the timely discharge of the liquid in the drainage cavity 41 from the liquid outlet 44 by negative pressure.

[0047] Furthermore, the inner end of the mounting cavity 102 is connected to the front tank 101 via a through hole 103, and the outer end of the mounting cavity 102 is open to receive the rear tank body 40. A positioning portion (not shown) can be provided on the end surface of the mounting cavity 102, and a mating portion (not shown) can be provided on the rear tank body 40 to mate with the positioning portion to position and install the rear tank body 40.

[0048] In a specific embodiment, a positioning protrusion can be provided on the outer end surface of the installation cavity 102 to serve as a positioning portion, and a positioning groove can be provided on the rear sump body 40 to match the positioning protrusion as a mating portion. When the rear sump body 40 is installed, the positioning protrusion and the positioning groove cooperate to position the rear sump body 40. In this way, the assembly difficulty of the rear sump body 40 can be reduced and the assembly time can be saved.

[0049] In other embodiments, a positioning groove may be provided on the outer end surface of the installation cavity 102 to serve as a positioning portion, and a positioning protrusion adapted to the positioning groove may be provided on the rear sump body 40 to serve as a mating portion. When the rear sump body 40 is installed, the positioning protrusion and the positioning groove are mated to position and install the rear sump body 40. This can also reduce the difficulty of assembling the rear sump body 40 and save assembly time.

[0050] In summary, the test kit of this embodiment has a simple structure, a novel fixing method for the microporous sheet 30 , is easy to process, has low assembly difficulty, and has high reliability.

[0051] In the second embodiment, the present application also provides a kit, such as Figure 1-Figure 5 As shown, Figure 4 yes Figure 1 A schematic structural diagram of an embodiment of the rear cell body in the kit shown, Figure 5 yes Figure 1 A schematic structural diagram of another embodiment of the rear cell body in the test kit shown in the figure, the test kit of this embodiment includes an impedance detection cell (not shown in the figure), which is used to perform impedance detection on the sample to be detected, and the impedance detection cell includes a rear cell body 40 and a rear cell electrode 50, and the rear cell electrode 50 is embedded in the rear cell body 40. The rear cell electrode 50 and the rear cell body 40 can be integrally injection molded or detachably connected.

[0052] The back cell body 40 is formed with a drainage cavity 41. The back cell electrode 50 is disposed on the back cell body 40 and extends into the drainage cavity 41. The inner end surface of the back cell electrode 50 is convex. Specifically, the inner end of the back cell electrode 50 can be configured as a hemispherical shape. The inner end surface of the back cell electrode 50 is the end surface of the back cell electrode 50 that extends into the drainage cavity 41. The convex structure of the back cell electrode 50 is described in the first embodiment and will not be repeated here.

[0053] Furthermore, if Figure 4 As shown, the rear cell body 40 includes a bottom wall 43 and a side wall 42. The bottom wall 43 is connected to the side wall 42 to form a drainage cavity 41. The rear cell electrode 50 is arranged on the bottom wall 43. The side wall 42 is provided with a liquid outlet 44, which is connected to the drainage cavity 41 and is used to discharge the gas or liquid in the drainage cavity 41. Figure 4 In the illustrated embodiment, the bottom wall 43 is a plane, and the plane on which the bottom wall 43 lies is inclined relative to the side wall 42. The liquid outlet 44 is provided at the intersection of the bottom wall 43 and the side wall 42, and is located on the side away from the microporous sheet 30. In this embodiment, the bottom wall 43 is provided as an upwardly inclined plane and serves as a drainage surface, allowing the liquid to flow through the microporous sheet 30 to the rear cell electrode 50 and then be drained by the bottom wall 43 to the liquid outlet 44, facilitating the liquid outflow from the liquid outlet 44, avoiding liquid accumulation, reducing signal disturbances caused by particle vortexing near the rear cell electrode 50, and improving detection reliability.

[0054] In addition, the liquid outlet 44 is located at the intersection of the side wall 42 and the bottom wall 43. In this way, the end of the slope formed by the bottom wall 43 is close to the liquid outlet 44. When the liquid in the drainage cavity 41 finishes draining through the bottom wall 43, it will flow directly out of the liquid outlet 44, further reducing the retention of the liquid.

[0055] In another embodiment, Figure 5 As shown, in this embodiment, the bottom wall 43 also serves as a drainage surface to drain the liquid within the drainage cavity 41. Specifically, the distance between the bottom wall 43 and the microporous sheet 30 gradually decreases from the location where the back cell electrode 50 is disposed on the bottom wall 43 toward the edge of the bottom wall 43. In other words, the bottom wall 43 may be shaped like a bell mouth to drain the liquid within the drainage cavity 41.

[0056] Furthermore, if Figure 5 As shown, the liquid outlet 44 is arranged at the intersection of the bottom wall 43 and the side wall 42, that is, the liquid outlet 44 can be arranged close to the bottom wall 43. In this way, when the liquid in the rear pool finishes draining through the bottom wall 43, it will flow directly out of the liquid outlet 44, reducing the retention of the liquid.

[0057] Furthermore, the maximum distance between the inner end surface of the rear cell electrode 50 and the bottom wall 43 is less than 0.5 mm. The inner end surface of the rear cell electrode 50 protrudes from the inner surface of the bottom wall 43 by a small distance, thereby reducing the phenomenon of liquid stuck in the rear cell electrode 50.

[0058] Furthermore, the distance between the inner end surface of the rear cell electrode 50 and the microporous sheet 30 is not less than 5 mm, so as to prevent the microporous sheet 30 from being too close to the rear cell electrode 50, thereby providing the liquid with sufficient buffer distance and facilitating the negative pressure to timely discharge the liquid in the drainage cavity 41 from the liquid outlet 44.

[0059] Optionally, the rear cell electrode 50 is disposed at the center of the bottom wall 43 , and the rear cell electrode 50 and the drainage cavity 41 are coaxially disposed. In this way, the demolding operation after the mold is formed is facilitated.

[0060] In the above embodiment, the structure of the impedance detection cell is novel, the inner end face of the rear cell electrode 50 is set to a convex surface, and the bottom wall 43 of the rear cell body 40 forms a drainage surface to guide the liquid to the liquid outlet 44. This structural improvement facilitates the outflow of liquid from the liquid outlet and can reduce the effects of signal disturbances caused by particle recoil and vortex, thereby improving the sample detection accuracy and the accuracy of the detection results.

[0061] In the third embodiment, the present application also provides a kit, see Figure 6 and Figure 7 As shown, Figure 6 is a schematic structural diagram of another embodiment of the kit provided by the present application, Figure 7 yes Figure 6 The kit is shown as an exploded schematic diagram. The kit includes a box body 10. The box body 10 includes a first box body 11 and a second box body 12. The first box body 11 and the second box body 12 are detachably connected.

[0062] The first box body 11 is provided with a first detection cell position, which is used for electrical impedance detection. The second box body 12 is provided with a second detection cell position, which is used for optical detection. In this embodiment, the first detection cell position can be used for WBC detection and RBC detection, and the second detection cell position can be used for detection of specific proteins. In other embodiments, the second detection cell position can also be used in conjunction with other biochemical tests, immunoassays, etc.

[0063] In this embodiment, the first box body 11 and the second box body 12 are detachably connected. This facilitates storage and transportation of the box body 10. In other embodiments, the first box body 11 and the second box body 12 can also be integrally formed to improve the reliability of the connection between the first box body 11 and the second box body 12.

[0064] exist Figure 6 and Figure 7 In the illustrated embodiment, at least one slot 111 is provided on one side of the first housing 11, and a protrusion 121 is provided on one side of the second housing 12 to cooperate with the slot 111. The slot 111 and the protrusion 121 cooperate to attach the second housing 12 to the first housing 11. This arrangement provides a simple connection structure between the first and second housings 11, 12, and facilitates assembly and disassembly. Preferably, the number of slots 111 and protrusions 121 can be multiple to provide a more secure connection between the second housing 12 and the first housing 11.

[0065] Furthermore, the side of the card slot 111 away from the first box body 11 is configured as a constriction, that is, the area of ​​the opening of the side of the card slot 111 away from the first box body 11 is smaller than the area of ​​the bottom wall of the side of the card slot 111 close to the first box body 11. In this way, the second box body 12 can be effectively prevented from falling off from the first box body 11.

[0066] Furthermore, the cross-sectional shape of the slot 111 along the first plane can be a trapezoid, wherein the first plane is a plane perpendicular to the thickness direction of the first box body 11. In this way, the connection portion between the first box body 11 and the second box body 12 has a regular shape, which facilitates the processing of the box body 10.

[0067] In another embodiment, Figure 8 and Figure 9 As shown, Figure 8 is a schematic structural diagram of another embodiment of the kit provided by the present application, Figure 9 yes Figure 8 As shown in the exploded schematic diagram of the reagent kit, specifically, a hanger 112 is provided on one side of the first box body 11 , and the edge of the second box body 12 is hung on the hanger 112 .

[0068] Specifically, if Figure 9As shown, the hanger 112 is formed with a hanging hole 1120, and the second box body 12 is inserted into the hanging hole 1120, and the outer edge of the second box body 12 is supported on the hanger 112. This method can make the force on the second box body 12 more uniform and can make the second box body 12 more stably fixed on the hanger 112.

[0069] Furthermore, the cross-section of the hanging hole 1120 can be rectangular, circular, trapezoidal, triangular, or irregularly shaped. The shape of the hanging hole 1120 can be adapted to the shape of the second box body 12. For example, when the second box body 12 is rectangular, the shape of the hanging hole 1120 is also set to be rectangular.

[0070] In other embodiments, at least two edges of the second box body 12 are suspended from the hanger 112, thereby supporting the second box body 12 via the hanger 112. This approach can simplify the structure of the box body 10 and facilitate production. For example, the hanger 112 can directly include two or three support rods for supporting the edges of the second box body 12 at two or three ends, thereby also saving material costs.

[0071] Furthermore, if Figure 7 and Figure 8 As shown, the first detection pool position may include a front pool 101, and the front pool 101 may be provided with two groups, one for cooperating with white blood cell detection and the other for red blood cell detection. The first detection pool position may also include at least one pipette tip placement pool 113, a diluent pool 114, a hemolytic agent pool 115, and a sample accommodating pool 116, wherein the pipette tip placement pool is used to accommodate the pipette tip 60, the diluent pool 114 is used to encapsulate the diluent, and the hemolytic agent pool 115 is used to encapsulate the hemolytic agent; the first detection pool position may also include a sample dilution pool 117, and the sample dilution pool 117 is used for sample dilution. Among them, multiple pool positions are arranged in a straight line. This method can facilitate the pipetting device to move a shorter path during automated detection, wherein the pipetting device is used to transfer and mix the liquids in each pool body.

[0072] To ensure the light transmittance of the material, the first housing 11 can be made of transparent PP (Polypropylene). In other embodiments, the forebay 101 can also be provided with a light-transmitting detection window (not shown) for cooperating with optical detection. The light transmittance and smoothness of the light-transmitting detection window can be the same as or higher than other parts of the forebay 101.

[0073] Furthermore, the second detection cell includes several placement holes 123, which are used to place the optical detection cup assembly (not shown) for optical detection. The placement holes 123 may include a first placement hole 121 and a second placement hole 122, which are spaced apart. The optical detection cup assembly includes an optical measuring cup (not shown) and a reagent cup (not shown). The optical measuring cup can be used for detecting specific proteins. The optical measuring cup can be made of transparent PC (Polycarbonate), and the reagent cup is used to store reagents. The first placement hole 121 can be used to place the optical measuring cup, and the second placement hole 122 can be used to place the detection cup.

[0074] The first placement hole 121 and the second placement hole 122 have different shapes. For example, the first placement hole 121 can be circular, and the second placement hole 122 can be rectangular, for the purpose of distinction.

[0075] Optionally, the second detection pool can be used to place two or more groups of optical detection cup assemblies, that is, the number of the first placement holes 121 and the second placement holes 122 can both be two or more groups for testing different items.

[0076] The box body 10 of the reagent kit in the above embodiment is detachably connected, and the assembly and disassembly processes are simple, making transportation convenient.

[0077] In the fourth embodiment, the present application also provides a kit, please refer to Figure 10 As shown, Figure 10 FIG2 is a schematic diagram of another embodiment of the kit provided herein. The kit of this embodiment includes a box body 10 and at least two pipette tips 60. The box body 10 is provided with at least two pipette tip placement wells, each for placing the at least two pipette tips 60. The pipette tips 60 are used to be assembled on a pipette to facilitate pipetting operations.

[0078] Each pipette head 60 has a certain volume. During the pipetting operation, the sample liquid or reagent liquid will remain in the pipette head 60. The volumes of at least two pipette heads 60 in this embodiment are different. For example, the test kit can be configured with two pipette heads 60, and the volumes of the two pipette heads 60 are different. In actual use, different sample liquids or reagent liquids can use pipette heads 60 of different volumes. For example, when 10 μL of liquid needs to be pipetted, a pipette head 60 with a capacity of 10 μL to 20 μL can be selected for pipetting to ensure the accuracy of the pipetting. In this way, the sampling accuracy can be improved, thereby improving the accuracy of the sample detection results.

[0079] Furthermore, if Figure 10As shown, the pipette tip 60 includes a tube body 61 and a fixing portion 62. The fixing portion 62 is fixed to the outer periphery of the tube body 61. The tube body 61 is formed with a liquid suction port 63 for sucking / discharging liquid. The fixing portion 62 is located on a side of the tube body 61 away from the liquid suction port 63. The fixing portion 62 is used to fix the tube body 61 in the pipette tip placement pool 113. The fixing portion 62 of pipette tips 60 of different volumes has different shapes, so that the pipette tips 60 can be distinguished by the shape of the fixing portion 62 to prevent the wrong pipette tip 60 from being selected. In other embodiments, the fixing portion 62 of pipette tips 60 of different volumes can also have the same shape to facilitate the production of the pipette tips 60.

[0080] The inner diameters of the pipette tip placement wells 113 on the box body 10 can be the same or different. For example, to facilitate identification, at least two pipette tip placement wells 113 can have different inner diameters to accommodate different pipette tips 60. Specifically, a pipette tip 60 with a larger volume can be inserted into the pipette tip placement well 113 with a larger inner diameter, while a pipette tip 60 with a smaller volume can be inserted into the pipette tip placement well 113 with a smaller inner diameter.

[0081] In order to enable the pipette tip 60 to adapt to pipette tip placement wells 113 of different inner diameters, the fixing portion 62 of the pipette tip 60 can be configured in a stepped shape. Specifically, the fixing portion 62 of the pipette tip 60 includes at least two steps, at least two of which are parallel and spaced apart toward the side away from the liquid aspiration port 63, and the outer diameters of the at least two steps gradually increase toward the direction away from the liquid aspiration port 63. By configuring the fixing portion 62 as steps of different sizes, the pipette tip 60 can adapt to pipette tip placement wells 113 of different inner diameters. In this way, the insertion and installation of the pipette tip 60 is not restricted by the inner diameter of the pipette tip placement well 113, thereby facilitating the removal and placement of the pipette tip 60.

[0082] When the pipette tip 60 is placed in the pipette tip placement pool 113 , the tube body 61 is inserted into the pipette tip placement pool 113 , and the pipette tip 60 can be supported on the surface of the box body 10 by the fixing portion 62 .

[0083] Furthermore, a sink 1131 may be provided at the opening of the pipette tip placement pool 113 . When the pipette tip 60 is placed in the pipette tip placement pool 113 , the fixing portion 62 may be supported on the sink 1131 to support the pipette tip 60 through the sink 1131 .

[0084] Optionally, the lengths and / or calibers of the pipette tips 60 with different volumes are also different, so as to facilitate distinguishing between different pipette tips 60. For example, the length / caliber of the pipette tip 60 with a smaller volume is also smaller, so as to facilitate distinguishing between different pipette tips 60.

[0085] Furthermore, a hydrophobic coating may be provided on the inner wall of the pipette tip 60 to prevent liquid from being attached to the pipette tip 60 and to improve the sample addition accuracy of the pipette tip 60 .

[0086] Furthermore, the pipette head 60 may be provided with an identification such as an electronic tag, a QR code or a barcode, etc., to record relevant parameters of the pipette head 60 .

[0087] The kit of the above embodiment can improve the sample addition accuracy, thereby improving the accuracy of sample detection results.

[0088] In the fifth embodiment, the present application also provides a POCT (point-of-care testing) blood cell analyzer, please refer to Figure 11 As shown, a structural schematic diagram of an embodiment of a pipette and a pipette tip in a POCT blood cell analyzer provided in the present application is provided. The POCT blood cell analyzer includes a pipette 70, and the end of the pipette 70 is used to cooperate with the pipette tip 60 of the above embodiment to perform pipetting operations.

[0089] Specifically, a suction tip 71 is provided at the end of the pipette 70, and the suction tip 71 is used to be connected to the pipette head 60. The pipette head 60 has a certain volume. During the pipetting operation, the sample liquid or reagent liquid will remain in the pipette head 60 and will not enter the interior of the pipette 70. After completing the aspiration, movement, and spitting operations and needing to replace the sample liquid or reagent liquid, the used pipette head 60 can be discarded and the unused pipette head 60 can be reinstalled, so that the new sample liquid or reagent liquid can be pipetted, and the pipette 70 will not be contaminated. Therefore, there is no need to clean the pipette 30 after each use, which eliminates the need for complex cleaning components and cleaning processes, thereby improving detection efficiency.

[0090] Furthermore, the suction head 71 at the end of the pipette 70 is arranged in a stepped shape to adapt to pipette tips 60 of different calibers. In this way, pipette tips 60 of different calibers can be connected by one suction head 71 to simplify the pipetting process and save material costs.

[0091] The present application also provides a point-of-care testing (POCT) blood cell analyzer, comprising a test kit according to any of the aforementioned embodiments and a detection base adapted therefor, for use in analyzing blood samples. The specific structure of the test kit is described in the accompanying drawings and accompanying text of the aforementioned embodiments, and will not be further detailed here.

[0092] The above is only an implementation method of the present application and does not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the description and drawings of this application, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A kit, characterized in that The kit comprises: The box body includes a front pool and a mounting cavity communicated with the front pool; A sealing ring is provided in the installation cavity and is located on a side of the installation cavity close to the front pool. A microporous sheet is provided with micropores that allow cells to pass through one by one. The microporous sheet is arranged in the mounting cavity and is located on the side of the sealing ring away from the front pool. The rear pool body is arranged in the installation cavity, the rear pool body abuts against the side of the microporous sheet away from the sealing ring, and is used to fix the microporous sheet and the sealing ring on the box body. The rear pool body is formed with a drainage cavity, and the front pool and the drainage cavity are connected through the micropores. The outer end surface of the installation cavity is provided with a positioning protrusion, and the rear pool body is provided with a positioning groove, and the positioning protrusion cooperates with the positioning groove to position the rear pool body; or, the outer end surface of the installation cavity is provided with a positioning groove, and the rear pool body is provided with a positioning protrusion, and the positioning protrusion cooperates with the positioning groove to position the rear pool body.

2. The kit according to claim 1, wherein The box body includes a through hole connecting the front pool and the installation cavity, The ratio of the diameter of the microporous sheet to the diameter of the via hole is not less than 1.7, and the diameter of the microporous sheet is not greater than the inner diameter of the mounting cavity.

3. The kit according to claim 2, wherein The sealing ring is loosely fitted into the mounting cavity.

4. The kit according to claim 1, wherein The rear cell body is bonded to the box body, and the microporous sheet and the sealing ring are bonded to the box body; or, The rear cell body is bonded to the box body, and the microporous sheet and the sealing ring are bonded to the rear cell body.

5. The kit according to claim 1, wherein The front cell is provided with a front cell electrode, and the rear cell body is provided with a rear cell electrode extending toward the drainage cavity. The front cell electrode and the rear cell electrode are respectively spaced apart and located on both sides of the microporous sheet, and the inner end surface of the rear cell electrode is a convex surface.

6. The kit according to claim 5, characterized in that The back cell body includes a bottom wall and a side wall, wherein the bottom wall is connected to the side wall to form the drainage cavity, and the back cell electrode is arranged on the bottom wall. The side wall is provided with a liquid outlet, which is connected to the drainage cavity. The bottom wall is a plane, which is inclined to the side wall. The liquid outlet is provided at the intersection of the bottom wall and the side wall and is located on the side away from the microporous sheet.

7. The kit according to claim 5, characterized in that The back cell body includes a bottom wall and a side wall, wherein the bottom wall is connected to the side wall to form the drainage cavity, and the back cell electrode is arranged on the bottom wall. From the position where the rear cell electrode is provided on the bottom wall to the edge of the bottom wall, the distance from the bottom wall to the microporous sheet gradually decreases.

8. The kit according to claim 1, wherein One side surface or both sides of the microporous sheet are provided with a concave guide surface around the micropores.

9. A POCT blood cell analyzer, characterized in that: The POCT blood cell analyzer comprises the test kit according to any one of claims 1 to 8 and a detection seat matched with the test kit, and is used for analyzing and detecting blood samples.

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