80 results about "Blood cell analysis" patented technology

The present application provides a test kit and a POCT blood cell analyzer. The test kit includes a box body, a sealing ring, a microporous sheet, and a back pool body. The box body includes a front pool and a mounting cavity connected to the front pool; the sealing ring is arranged in the mounting cavity and is located on the side of the mounting cavity close to the front pool; the microporous sheet is provided with micropores that allow cells to pass through one by one; the microporous sheet is arranged in the mounting cavity and is located on the side of the sealing ring away from the front pool; the back pool body abuts against the side of the microporous sheet away from the sealing ring, and is used to fix the microporous sheet and the sealing ring on the box body; the back pool body is formed with a drainage cavity, and the front pool and the drainage cavity are connected through micropores. The test kit provided by the present application has a simple structure, is easy to assemble, and is conducive to the processing of the test kit.

A blood cell analyzer (100) and a blood cell analysis method (300). The blood cell analysis method (300) comprises: sucking a blood sample to be tested; preparing a first determination sample and obtaining first optical information; preparing a second determination sample and obtaining second optical information; generating a first leukocyte classification scatter diagram and a second leukocyte classification scatter diagram; determining a first counting result of neutrophils of the to-be-detected blood sample based on the first leukocyte classification scatter diagram and the second leukocyte classification scatter diagram; determining an actual distribution area of neutrophils from the first leukocyte classification scatter diagram; based on a reference distribution region of neutrophils of a reference leukocyte classification scatter diagram of the normal blood sample, determining a leaf-division nucleophore distribution region from the actual distribution region; determining a second counting result of the cells falling into the leaf-separating nucleogranulocyte distribution area; and based on the first counting result and the second counting result, determining a counting result of the rod-shaped nuclear granulocytes in the to-be-detected blood sample.

The invention relates to the technical field of blood cell analysis, and particularly discloses a blood cell analysis system and method based on computer vision, which uses an image recognition and generation technology based on computer vision to carry out grid division and semantic feature extraction on blood image data. Performing global text generation and semantic coding on each blood image data grid block feature after semantic extraction so as to perform adjustment response aggregation on attention of different blood local areas in each blood image data grid block feature according to blood image text description semantic features; therefore, intelligent optimization of the blood image data is realized. In this way, the complex deep-layer mutual influence association relationship between cells can be captured, so that a more accurate diagnosis basis is provided. Meanwhile, the processing strategy can be dynamically adjusted according to the specific description content of the blood image so as to better adapt to diversified samples, and the method does not only depend on fixed preprocessing parameters.

The utility model belongs to the technical field of medical instruments, and particularly relates to a blood extraction device for a hematology analyzer, which comprises an electric telescopic frame and a detection module, the electric telescopic frame is positioned on one side of a hematology analyzer main body and is connected with a base, a plurality of clamping grooves I are formed in the electric telescopic frame, and the detection module is connected with the electric telescopic frame. An extraction needle cylinder is detachably installed in the first clamping groove, an extraction needle head is arranged at one end of the extraction needle cylinder, a piston is movably connected to the interior of the extraction needle cylinder in a sealed mode, the detection cylinder is driven to descend through stretching and retracting of the electric telescopic frame, and in the descending process of the detection cylinder, after the detection module detects the layering position of plasma and blood cells, the extraction needle head is connected to the extraction needle cylinder; the controller controls the electric telescopic frame to move, at the moment, the push rod is pulled, the extraction needle head is just located above the layering position, the plasma is extracted into the needle cylinder, one-time plasma extraction is achieved through accurate detection of the detection module, and waste of the plasma is reduced as much as possible.

The invention discloses a hematology analyzer and a platelet numerical value measuring method, in the hematology analyzer, an analysis unit is used for receiving a pulse signal to generate a first image, receiving an optical signal to generate a second image, obtaining a platelet original numerical value based on the first image, obtaining an interference coefficient of a leukocyte fragment based on the second image, and obtaining the platelet numerical value based on the interference coefficient; and obtaining a platelet measurement value based on the platelet original value and the interference coefficient. Through the cooperation of the impedance module and the flow fluorescence module, the platelet original value in the blood cell sample and the interference coefficient of the leukocyte fragment are detected, and the accuracy of each step in the calculation process of the platelet measurement value is ensured. Meanwhile, the platelet measurement numerical value is calculated through the analysis unit based on the platelet original numerical value and the interference coefficient, so that the accuracy of the blood cell analyzer for measuring the platelet measurement numerical value is improved, the practicability of the blood cell analyzer is improved, and the use experience of a user on the blood cell analyzer is improved.

The invention discloses a hematology analyzer and a detection method for hole blockage of a flow chamber, in the hematology analyzer provided by the invention, in the process that sample liquid enters the flow chamber for sample detection, a control unit can also obtain signal parameters based on optical signals of the sample liquid flowing through the flow chamber; whether the flow chamber is blocked or not is judged, the safety of the flow chamber is improved, and the detection efficiency of an optical detection system on sample liquid is improved. Meanwhile, in the process of detecting the sample liquid, whether the flow chamber has the hole blocking problem or not can be judged, the step of judging the hole blocking of the flow chamber does not need to be additionally set, the operation efficiency of the hematology analyzer is improved, and the use experience of a user on the hematology analyzer is improved.

The present application provides a blood cell classification method, in which the impedance pulse signal of the blood sample to be classified is input into a trained target neural network classification model. The target neural network classification model is trained by a large number of impedance pulse signal samples with classification result label information. It has a strong pulse morphology feature extraction capability, so that the classification result of the impedance pulse signal can be determined based on the morphological characteristics of the impedance pulse signal of the blood sample to be classified. Compared with the existing technology, the classification result is more accurate, and it can identify the impedance pulse signal caused by interference factors, and can perform identification and classification based on the impedance pulse signal, with higher real-time performance. In addition, the present application also provides a blood cell analyzer and a storage medium.

The present disclosure relates to a method for white blood cell differential counting using whole blood in a fluid state, and the like. The method of the present disclosure does not include the drying and washing processes used in traditional blood cell analysis to provide a standard for multiple white blood cell analysis, thereby enabling rapid analysis with high repeatability and reproducibility even when large quantities of samples are being processed, and providing accurate test results including the numbers, ratios, and morphological discrimination for each type of white blood cells.

The application discloses a blood cell analyzer and a processing method of a red blood cell sample. In the blood cell analyzer, a control module grades the agglomeration degree of the red blood cell sample, and then processes the red blood cell sample according to the corresponding grade based on the agglomeration degree grade of the red blood cell sample, to obtain a detection result of the red blood cell sample. The control module can intelligently grade the agglomeration degree of the red blood cell sample, and process the red blood cell sample according to the corresponding grade based on the agglomeration degree grade of the red blood cell sample, so that the blood cell analyzer can automatically process the agglutinated red blood cell sample, the agglomeration effect of the red blood cell sample is reduced, the detection accuracy of the blood cell analyzer for the red blood cell sample is improved, the probability of missing detection in the manual report auditing process is reduced, the workload of manual disposal is reduced, time for accurately and quickly issuing a detection report is saved, and the user experience of the blood cell analyzer is improved.

The invention discloses a POCT (Point of Care Testing) hematology analyzer. In the application, the first bearing position is arranged on the sample injection module to bear the detection card for receiving the first to-be-detected sample, and the second bearing position is arranged on the first detection module to bear the detection card after receiving the first to-be-detected sample, so that the receiving and detection of the first to-be-detected sample are separately carried out at different bearing positions of different modules; the transfer module is arranged at the output end of the driving module of the preprocessing module, so that the driving module can drive the transfer module to transfer the detection card from the first bearing position to the second bearing position after driving the pipetting consumable through the loading module to transfer the first to-be-detected sample into the detection card at the first bearing position; therefore, the driving module is ingeniously used for driving the transfer module, and a driving device special for driving the transfer module is avoided, so that the overall structure of the analyzer is simpler, the production cost is reduced, and the analyzer is more compact and miniaturized in structure.

The invention provides a fluorescent dye for blood cell analysis. The fluorescent dye has the following structural general formula I, wherein X is CH2, C (CH3) 2, O, S or Se; y is-CH2-CH2 = CH-, or is selected from any one of the following structural formulas II, III and IV, and Z is an anion; r1 and R2 are respectively and independently selected from straight-chain or branched-chain C1-10 alkyl, benzyl and-(CH2) mNR3R4, the benzyl is optionally substituted by the following substituent groups: halogen, hydroxyl, sulfydryl and alkyl, and R1 and R2 are not benzyl at the same time; m is 1-10, and R3 and R4 are independently selected from H and straight-chain or branched-chain C1-6 alkyl groups respectively.

The hematology analyzer comprises a main machine and a puncture device, a front face cover is fixedly arranged at the front end of the main machine, a sampling groove of a concave structure is formed in the edge of the lower end portion of the front face cover, and the puncture device is fixedly arranged in the upper end portion of the front face cover. A sampling needle of the puncture device can vertically and downwards extend out of the front mask into the sampling groove; the control end of the puncture device is electrically connected with the host; a sampling key or a puncture test tube adapter is arranged on the inner wall of the sampling groove, and the sampling key or the puncture test tube adapter is electrically connected with the host. Compared with the prior art, the utility model realizes the switching between an automatic sampling mode and a manual sampling mode, and has the advantages of flexible conversion, stronger independence, convenience in installation and adjustment, efficient and convenient sampling and low cost; and operation is convenient, size is small, parts are few, and maintenance is convenient.

This application provides a method for using a POCT hematology analyzer, comprising: in response to a reagent kit being placed in a test chamber, controlling a pipette to move above the first mounting head reservoir of the reagent kit and descending to load the first mounting head; controlling the pipette to move to the diluent reservoir of the reagent kit to transfer the diluent into the pre-cell for impedance detection; controlling the pipette to transfer a sample into the pre-cell; mixing the test solution in the pre-cell; controlling the metal shielding cap to close with the test chamber to establish a connection between the rear cell and the pressure building system of the reagent kit; controlling the pressure building system to guide the test solution from the pre-cell into the rear cell; and detecting the test solution by impedance detection.

To reduce a work load by shortening a moving distance of an operator.SOLUTION: A composite analysis system 1 includes: a blood cell analyzer 2; a blood coagulation measurement device 3; and a transport device 4 having a transport path 50. The blood cell analyzer 2, the blood coagulation measurement device 3, and the transport device 4 are arranged at a position and orientation that allow at least one of access to a power supply operation reception unit of the blood cell analyzer 2 and the blood coagulation measurement device 3 from one side along the transport path 50, access to a consumable item setting unit of the respective devices from one side along the transport path 50, access to a terminal device of the respective devices from one side along the transport path 50, and access to a maintenance management target unit of the respective devices from one side along the transport path 50.SELECTED DRAWING: Figure 1

The application discloses a blood cell analyzer and a blood cell analysis method. The liquid storage assembly of the blood cell analyzer comprises a liquid storage cavity. The height of the inlet of the liquid storage cavity is less than the height of the outlet. The first end of the temporary storage mechanism of the liquid path emptying assembly is connected with the pressure supply mechanism. The second end of the temporary storage mechanism is connected with the inlet of the liquid storage assembly through the first control valve. The processor is used to control the pressure supply mechanism to switch the temporary storage mechanism to the negative pressure state in response to receiving the liquid draining instruction. The processor is also used to control the first control valve to conduct the liquid storage assembly and the temporary storage mechanism, so that the liquid in the liquid storage cavity is drained through the pipeline between the first control valve and the liquid storage assembly. The liquid in the liquid storage cavity can be drained through the pipeline between the first control valve and the liquid storage assembly by the temporary storage mechanism in the negative pressure state, the residual reagent in the liquid storage cavity is removed, the blockage failure caused by the evaporation and crystallization of the reagent is reduced or avoided, and the service life and reliability of the blood cell analyzer are improved.

The utility model discloses a built-in hemolytic agent bottle and hematology analyzer relates to medical instrument technical field, wherein the built-in hemolytic agent bottle includes solvent bottle, movable joint block and pipeline subassembly, solvent bottle includes mutually communicated bottle body and bottle cap, bottle body and bottle cap detachable connection, bottle cap far away from bottle body side is equipped with the movable mouth; the movable connector block is movably arranged at the movable opening, the movable connector block and the movable opening are arranged in a clearance mode, and the movable connector block is provided with a mounting hole which is formed in the thickness direction in a penetrating mode; the pipeline assembly is fixedly arranged at the mounting hole, one end of the pipeline assembly extends into the bottle body, and the other end of the pipeline assembly penetrates out of the mounting hole and is communicated with an external liquid system. According to the technical scheme, the problem that when a bottle cap of an existing built-in hemolytic agent bottle is screwed down, a pipeline is prone to being bent is solved.

The invention belongs to the technical field of biomedical detection, and discloses a blood cell analysis system based on image flow cytometry, which comprises a cell staining module, the dyeing module simultaneously comprises a first fluorescent probe capable of being specifically combined with components in a cell nucleus or enriched in the cell nucleus, a second fluorescent probe capable of being specifically combined with cell nucleus DNA and a third fluorescent probe capable of being specifically combined with a blood cell differentiation antigen; an image flow cytometer; and the image processing module is used for calculating the kernel detection rate and / or the average kernel number. According to the present invention, the combination of the specific types of the cell staining dyes is adopted, the image flow cytometry is matched, and the optimized multi-dimensional fluorescence staining combination and the matched automatic image analysis process are adopted to achieve the synchronous, high-throughput and accurate quantitative analysis of the blood cell immune phenotype and the cell morphology key characteristics at the single cell level;

The invention relates to the technical field of animal blood cell detection and analysis, in particular to a method for an animal blood cell analyzer to recognize platelet aggregation, which comprises the following steps: after the animal blood cell analyzer performs pretreatment and fluorescent staining on a blood sample in a white blood cell DIFF channel, acquiring three signals of blood cells through laser flow cytometry; two scatter diagrams are drawn according to the three signals, platelet aggregation particles are identified in a certain area range in the two scatter diagrams, when the number exceeds a threshold value, an alarm is given, and normal leukocytes cannot fall in the area. The good alarm function of the instrument provides more accurate prompts for clinical diagnosis, and clinical appropriate microscopic examination of platelets is facilitated. The cost is saved, extra testing is not needed, and the alarm function can be achieved in the leukocyte DIFF channel in the blood routine testing mode. The platelet aggregation region is identified, the influence of platelet aggregation on the total number of leukocytes is eliminated, and the leukocyte value is more accurate.

This application relates to a sample analysis system and a method for detecting platelet abnormalities. The sample analysis system includes: a blood cell analysis device, comprising a sample preparation unit and a detection unit, wherein the sample preparation unit is configured to prepare a test sample from a blood sample to be tested, and the detection unit is configured to test the test sample to obtain a first platelet detection result; a smear preparation device, configured to prepare a smear of the test sample from the blood sample to be tested; a cell image capturing device, configured to capture cell images of the test sample smear to obtain cell images of the blood sample to be tested, and to analyze the cell images to obtain a second platelet detection result; and a data processing device, configured to determine whether platelet abnormalities exist in the blood sample to be tested based on the first and second platelet detection results, and to output a platelet abnormality prompt when a platelet abnormality is determined to exist. This enables more accurate identification of platelet abnormalities.

The invention discloses an intelligent blood sample identifying and sorting system, and relates to the technical field of sorting. The system comprises a sorting central control management server, a composite robot unit, a sample receiving unit, a sample identifying and sorting unit, a recovery processing unit, a blood cell analyzer, a blood clotting instrument, a serum analyzer and a plasma analyzer, the sorting central control management server is respectively connected and communicated with the composite robot unit, the sample receiving unit, the sample identifying and sorting unit and the recovery processing unit; the sorting central control management server, the hematology analyzer, the blood clotting instrument, the serum analyzer and the plasma analyzer are all used for being connected and communicated with a hospital clinical laboratory information system LIS; through the arrangement of distributed systems such as the sorting central control management server, the composite robot unit, the sample receiving unit, the sample identifying and sorting unit, the recovery processing unit, the hematology analyzer, the blood clotting instrument, the serum analyzer and the plasma analyzer, the efficiency is improved, and the occupied area is saved.

The utility model discloses a blood cell and fluorescence immunoassay analyzer, which relates to the technical field of medical instruments and comprises a frame, a whole blood sample component, an immunoreagent component, a blood cell counting component, an immunoassay component and a sampling component. The rack is provided with a first station, a second station, a third station and a fourth station which are arranged at intervals, the whole blood sample assembly is located at the first station, the immunoreagent assembly is located at the second station, the blood cell counting assembly is located at the third station and used for classifying and counting whole blood samples and obtaining HCT values of the whole blood samples, and the immunoassay assembly is located at the fourth station and used for analyzing the HCT values of the whole blood samples. The sampling assembly is used for carrying out fluorescence immunoassay on a whole blood sample, and the sampling assembly is movably arranged on the rack and is used for transferring a corresponding sample or reagent. According to the scheme, blood cell analysis and immunoassay are integrated in one instrument, and a sample in blood cell analysis can be synchronously applied to immunoassay, so that the cost is saved, and the analysis efficiency is improved.

Disclosed in the present application is a POCT hematology analyzer. The POCT hematology analyzer comprises: a cell counting assembly movable relative to a reagent kit, wherein the cell counting assembly comprises a main body provided with a front chamber and a rear chamber; a microporous sheet configured to isolate the front chamber from the rear chamber and provided with a micropore, the front chamber being in communication with the rear chamber by means of the micropore; and a first electrode, which penetrates and is arranged in the rear chamber and configured to be electrically connected to a test sample in the rear chamber, wherein the range of the shortest distance between the portion of the first electrode that penetrates and is arranged in the rear chamber and the micropore is 2-20 mm. By the above means, it is possible to prevent the first electrode from being too far away from or too close to the micropore, thereby reducing interference from other cells, minimizing bubble formation, and accordingly improving the accuracy of test results of the POCT hematology analyzer.

The invention relates to a blood cell analyzer and a blood cell analysis method. Sucking a to-be-detected blood sample; mixing a part of the blood sample to be detected, a hemolytic agent and a first fluorescent staining agent to prepare a first measurement sample, and detecting first optical information of particles in the first measurement sample; mixing the other part of the blood sample to be measured, the diluent and a second fluorescent staining agent to prepare a second measurement sample, and detecting second optical information of particles in the second measurement sample; acquiring first leukocyte classification information based on the first optical information; when it is judged that classification abnormity exists in the first leukocyte classification scatter diagram, second leukocyte classification information is obtained based on the second optical information, and the second leukocyte classification information is used for correcting the first leukocyte classification information. According to the invention, an accurate leukocyte classification result can be obtained.

The utility model provides a blood collection tube mounting mechanism of a blood cell analyzer, which comprises a mounting base, a blood collection tube transposition mechanism arranged on the left side of the mounting base, a sample output mechanism arranged on the right side of the mounting base, and a left support plate fixedly mounted on the left side of the mounting base, a lifting seat is fixedly mounted at the top of the left supporting plate, and a connecting seat is detachably connected to the top of the lifting seat. Through the design of the blood collection tube transposition mechanism, a plurality of soft blood collection tubes can be mounted and supported, meanwhile, the soft blood collection tubes which are not inspected can be driven to an inspection station, and through the design of the sample output mechanism, the soft blood collection tubes can be extruded, so that samples in the soft blood collection tubes are output from the bottom and then fall down under the action of gravity; the blood cell analyzer is used for testing, namely, the function of automatically switching and testing a plurality of samples is achieved, the automation degree of the instrument is improved, and the efficiency of other work of an operator is improved.

The present application relates to the technical field of biological cell collection and preservation, in particular to a peripheral blood immune cell efficient collection and low-temperature storage method, comprising: collecting peripheral blood samples and obtaining donor health status information, generating a sample cell composition atlas containing lymphocyte subpopulation absolute count percentage, monocyte purity and granulocyte contamination ratio through full-automatic blood cell analysis, determining the target immune cell optimal collection amount and adaptive collection process through a preset data analysis model, enriching target cells through gradient density centrifugation and specific magnetic bead sorting, comparing and judging supplementary collection by combining cell quality inspection data and expected collection amount, and then calculating individualized cryopreservation liquid formula, cooling program and storage strategy through a low-temperature storage optimization algorithm. The method can realize precise individualized adaptation of the collection process and storage parameters, and improve the cell enrichment purity and the cell state stability after low-temperature storage.

The invention relates to the technical field of blood analysis, in particular to a scatter diagram-based lipid particle identification method and a blood cell analyzer. The method comprises the following steps: extracting lipid particle characteristics in a three-dimensional scatter diagram of a blood sample to obtain sample curve characteristics and particle number characteristics, and obtaining a lipid particle identification result of the three-dimensional scatter diagram based on the sample curve characteristics and the particle number characteristics; and outputting alarm information under the condition that the lipid particle recognition result represents that the lipid particles exist in the three-dimensional scatter diagram. By adopting the method, the cell counting accuracy can be improved.

The invention provides a platelet simulant as well as a preparation method and application thereof, the preparation method comprises the following steps: acquiring platelets from whole blood of mammals, fixing the platelets by using a fixing agent, and then storing the platelets in a preservative solution; the fixing agent comprises 0.01-1% (v / v) of formaldehyde, 0.01-1% (v / v) of glutaraldehyde, 0.1-2% (v / v) of a dispersing agent and 0.1-10 g / L of an anti-polymerization agent. According to the preparation method, platelets are obtained from mammal whole blood, raw material sources are easy to obtain, and the cost is low; the preparation method is simple to operate and beneficial to large-scale production of the product; the prepared platelet simulant is stable in performance, small in batch difference and good in simulation performance, and can be simultaneously applied to an electrical impedance channel, an optical channel and a fluorescent channel of a hematology analyzer.

This invention relates to the field of hematology analysis technology, and in particular to a diluent for hematology analysis, its preparation method, and its uses. The diluent comprises the following components: 0.5-15 g / L of inorganic salt, 0.01-5.0 g / L of preservative, and 0.1-0.5 g / L of stabilizing agent; the solvent of the diluent is water; the diluent also includes an anti-precipitant, selected from one or more of salt-based and organic anti-precipitants; the salt-based anti-precipitant includes one or more of thiocyanate and nitrate, with a content of 2.0-5.0 mmol / L; the organic anti-precipitant includes one or more of trehalose, mannitol, and amino acid ionic liquids, with a content of 4.0-8.0 mmol / L. In this diluent, the components work synergistically to reduce the maintenance cost of the hematology analyzer, increase its service life, and improve detection efficiency.

This utility model provides a reagent bottle assembly and a blood cell analyzer incorporating the same. The reagent bottle assembly, used in the blood cell analyzer, includes: a reagent bottle body; a flexible tube with an input end and an output end, the input end being located inside the reagent bottle body and the output end being located outside the reagent bottle body; and a suction head located inside the reagent bottle body and positioned at the input end. A first end of the suction head is connected to the input end, and a second end of the suction head has an opening. The suction head has a suction channel connecting the input end and the opening. The center of gravity of the suction head is located on the side of a preset plane away from the input end, wherein the preset plane is half-height plane of the suction head. The technical solution of this application effectively solves the problem of poor suction effect in related technologies.

The invention relates to a monocyte simulation composition, a preparation method, a quality control substance and a quality control method. The mononuclear cell simulation composition comprises mononuclear cell simulation particles stored in a preservation solution, the volume ratio of the mononuclear cell simulation particles in the composition is 50% or above, the mononuclear cell simulation particles are obtained by treating neutrophils or lymphocytes separated from blood of mammals, and the mononuclear cell simulation particles are used for simulating the mononuclear cells or the lymphocytes. When the mononuclear cell simulation particle is tested by a hematology analyzer, the mononuclear cell simulation particle has the same or similar light scattering characteristics as human mononuclear cells, and the light scattering characteristics at least reflect the complexity of cell contents. The mononuclear cell simulation particles are independently prepared, so that the particles have good consistency and stability, and the obtained composition is small in batch-to-batch difference when serving as a quality control substance, so that the quality control of a hematology analyzer is facilitated, and the reliability of clinical examination of the hematology analyzer is improved.