159 results about "Blood cell analysis" patented technology

A sending mode and the sending device automatically for the blood cell analysis instrument are to convert the tube to send the sample. The auto sending sample device includes the tube pushing device, the mixing device, the scan device and puncturing device. The tube pushing device is connected to the mixing device, the scan device and puncturing device. It will combine the mixing equip and scan device into one mixing scan device. The invention makes tube blood mix under the situation that the tube doesn't escape from a tube, and have good mixing efficient. But the blood collecting can be completed under the situation that the tube doesn't escape from a work. So it makes the puncturing sample more convenient and simple. It can further shorten the blood sample entering time, have operation more simple, the equipments are more cleverly made and the manufacturing cost is cheaper and higher automation degree.

The invention belongs to the technical field of blood cell analysis, and particularly relates to a blood cell analysis chip, analysis meter and analysis method. The blood cell analysis chip disclosed by the invention comprises a white blood cell/hemoglobin analysis chip and a red blood cell/blood platelet analysis chip; the white blood cell/hemoglobin analysis chip performs white blood cell classification by adopting the sheath flow technology and the multi-angle laser polarized light scattering technology, and detects the concentration of hemoglobin by adopting the light absorption method; the red blood cell/blood platelet analysis chip counts the red blood cells and blood platelets respectively by adopting the electrical impedance technology and the floating threshold technology. According to the invention, the blood cell analysis chip is utilized to realize classification of blood cells, has the advantages that the structure is simple, the size is small, the cost is low, the operation is convenient, the easy maintenance and transportation are realized, the chip can be discarded after being used, and the like, satisfies the development requirements of miniaturization, integration and portability of an analysis instrument, and is particularly suitable for field detection, emergency analysis, household application and use of primary care departments.

The invention belongs to agents for medical diagnostic apparatuses, and in particular relates to an agent for a blood cell analyzer. The agent for the blood cell analyzer consists of a diluent, an EOI hemolytic agent, an LH hemolytic agent, an EOII staining solution and a cleaning solution. The agent for the blood cell analyzer is wide in range of use, suitable for many types of machines, suitable for detectors with machine types of BC5100/5180/5300/5380/5310/5390 and the like, and stable and accurate in detection result; the R value of a correlation coefficient after linear regression reaches more than or equal to 0.99 compared with a Mairui original blood cell agent and expresses relatively good relativity; and moreover, the agent is rich in raw material and low in price, and the raw materials are nontoxic and harmless and do not have threats on operators, so that the burden of the environment is lightened, and the raw materials are beneficial for popularization and application of the agent for the blood cell analyzer.

The invention relates to a hemolytic agent for a blood cell analysis device. The hemolytic agent is capable of cracking the erythrocytes in the blood, and the blood with cracked erythrocytes can be used for hemoglobin concentration determination. In addition, the invention also relates to a leukocyte classification reagent system which comprises the hemolytic agent and an inhibitor. After the hemolytic agent cracks the erythrocytes, the inhibitor can be added into the blood to prevent the hemolytic agent from damaging the morphology and structure of the leucocytes. Furthermore, the invention also relates to a method of classifying the leucocytes by using the reagent system.

A method for indentifying red cell fragments is used for blood cell analysis with cell counting by the resistance method, and comprises the following steps: A. counting white blood cells in a blood sample, drawing a white blood cell column diagram, and recording the full width at half maximum of each particle pulse during the process of particle pulse signal identification; B. locating an area A with possible existence of red cell fragments and an area B without any red cell fragments on the white blood cell column diagram, and determining whether the existence of red cell fragments interfering with white blood cell counting is true in the area A according to the comparison between the distribution of the full width at half maximum of particle pulse in the area A and in the area B; and C. in the case of disturbance caused by red cell fragments, determining the position, in which the interference peak of red cells ends, and correcting the count value of white blood cells according to the ending position of the interference peak of the red cells. The method achieves the effect of improving the accuracy of the result of white blood cell counting and the accuracy of white blood cell classification.

The invention discloses an integrated blood inspection platform and a using method thereof. The integrated blood inspection platform comprises a sample feeding center and inspection instruments, wherein each inspection instrument is fixed on a bracket; the sample feeding center is connected with the inspection instruments through a uniform interface; the sample feeding center comprises a sample feeding arm, a sample rack, a cleaning center, a temperature control system and a reagent rack; the inspection instruments comprise a blood rheology/ blood sedimentation instrument, a biochemical analyzer, a blood cell analyzer and a blood coagulation analyzer; the integrated blood inspection platform further comprises inspection modules corresponding to the inspection instruments, wherein the inspection modules are used for controlling the inspection instruments; and the inspection modules respectively comprise a blood rheology/ blood sedimentation instrument, a biochemical analysis module, a blood cell analysis module and a blood coagulation analysis module. The inspection devices of various detection methods are integrated into the detection modules with the uniform interface and integrated in the inspection platform, so that the project detection of various detection methods can be realized by using only one inspection platform, the spatial dimensions of the devices are greatly reduced, and the using cost is also reduced.

This invention relates to a blood cell analysis method based on machine vision. Inputting the blood sample into a computer by a camera to convert the color image into the black-and-white and then the binary one and scale the binary image; calculating the area and taking count of the scaled image to obtain the number of RBC, WBC and platelet. This invention has high efficiency and accuracy with low cost.

The invention relates to a laser system for blood cell analysis, flow cell analysis and body fluid analysis, which comprises a first laser source, a second laser source, a first condensation device, a second condensation device and a front receiving device and a lateral receiving device, wherein the first laser source transmits laser with first wavelength; the second laser source transmits laser with second wavelength; the first condensation device makes the laser transmitted by the first laser source condense and enter a sample room; the second condensation device makes the laser transmitted by the second laser source condense and enter the sample room; and the front receiving device and the lateral receiving device are used for collecting emergent light signals of the sample room. The laser system can reduce the interference of cell membranes on cell nucleus scattering in the process of detecting a cell sample, acquire more information of a detected object, reduce error detection and omission detection of the detected objects, and improve the detection accuracy of a detector.

A blood cell analyzer diluent and a hemolytic agent, are characterized in that one litre diluent is provided with 12.0-4.0g of sodium chloride, 2.0-10.0g of sodium sulfate, 0.8-0.5g of 1,3,2-methylol urea, 0.2-0.5g of copper sulfite, 3.0-8.0g of EDTA-2Na, 0.2-0.7g of Piperacillin Sodium, a borate buffering liquid toning the ph value to 7.2-7.8, and the balance is water; one litre hemolytic agent is provided with 0.8-5.0g of potassium chloride, 0-60.0g of dodecyl trimethyl ammonium chloride, 14.0-0g of octadecyl trimethyl ammonium bromide, 6.0-10.0ml of isopropanol, carbonate or alcaine buffering liquid toning the ph value to 7.2-7.8, and the balance is water. The inventive reagent can form stable hemoglobin derivatives, and the absorption spectrum curves are similar when lambada is 540nm, lambada is 504nm, which can satisfy the clinical inspection requirement; the reagent does not contain cyanide, azide, and has non-toxicity, which can effective improve working atmosphere of operating staff, and can reduce harm of toxicant to personal health.

The invention relates to a blood cell segmentation and recognition model construction method and a blood cell recognition method, and provides a blood cell segmentation model and recognition model construction method on one hand and a cell recognition method based on a blood cell segmentation model and a recognition model on the other hand. The blood image database is generated based on the full-view image, and the blood cell segmentation model is trained, so that the accuracy and comprehensiveness of data are ensured, and the segmentation accuracy of the blood cell segmentation model is improved. Full-view blood cell analysis is realized by utilizing a computer, so that the interference of human objective factors is greatly reduced, and the objectivity and consistency of an inspection result are improved. The blood cell segmentation model and the recognition model are intelligent, the software algorithm has a self-learning attribute, the training efficiency of the recognition model isgradually improved along with the increase of high-quality labeled images, and the software recognition and classification accuracy can be continuously optimized.

The invention belongs to agents for medical diagnostic apparatuses, and in particular relates to a buffer solution for an electrochemiluminescence immunity analyzer and a preparation method of the buffer solution. The buffer solution for the electrochemiluminescence immunity analyzer comprises the following components: potassium dihydrogen phosphate 4, tween-20, dodecyl polyglycol ether, polidocanol, tripropyl amine and citric acid; and the pH value of the buffer solution is 6.5-7.0. The buffer solution for the electrochemiluminescence immunity analyzer is simple to prepare and less in usage amount of tripropyl amine to reduce harms of tripropyl amine to bodies of operators and reduce the burden of the environment; the R value of a correlation coefficient after linear regression reaches more than 0.99 compared with an imported buffer solution for an electrochemical immunity analyzer and expresses relatively good relativity; and moreover, the buffer solution is low in production cost, and the selling price can be reduced by 30% compared with a reagent for an equivalent blood cell analyzer on the market, so that the buffer solution for the electrochemiluminescence immunity analyzer is beneficial for large-scale popularization and application.

The invention provides a green fluorescence cyanine dye, which has a structural general formula I shown in the specification, wherein in the formula, X is C(CH3)2, O, S or Se; R1 and R2 are independently selected form H, C1-18 alkyl groups, OR6, C1-6 alkyl group OR6 or halogen respectively; R3 is N(R5)(R7); R4 is selected from C1-18 alkyl groups, benzyl groups and substituted benzyl groups; the substituted benzyl groups are arbitrarily substituted by the following groups: C1-18 alkyl groups, CN, COOH, NH2, NO2, OH, SH, C1-6 alkoxy groups, C1-6 alkyl amino groups, C1-6 acylamino groups, halogen or C1-6 halogenated alkyl groups; R5 and R7 are independently selected from H or C1-18 alkyl groups respectively; R6 is H or C1-18 alkyl groups; and Y is negative ions. The fluorescence dye can be used for DNA (Deoxyribonucleic Acid) quantitative detection and biological dyeing, and is applied to the fields of nucleic acid marking, blood cell analysis, clinic medical diagnosis, immune analysis detection and the like.

The diluent for hemocyte analyzer includes sodium hydroxide 430-470 g/L, dimethylol urea 0.8-1.2 g/L, phosphoric acid 430-470 ml/L, non-ionic surfactant 0.3-0.7 g/L, procaine hydrochloride 0.09-0.13 g/L, anhydrous sodium sulfate 1.0-9.5 g/L, and sodium chloride 0.3-0.7 g/L except water. The diluent of the present invention may be used in various kinds of hemocyte analyzer.

The invention discloses a particle counting device. The particle counting device comprises a front pool part, a rear pool part, a counting component arranged between the front pool part and the rear pool part, wherein the rear pool part comprises a rear pool shell, a rear sheath inlet, a capturing pipe, a waste liquid outlet, a rear pool cleaning outlet and a rear pool; the rear pool is formed by an internal cavity of the rear pool shell; the rear sheath inlet is arranged on the rear pool shell and is communicated with the rear pool; one end of the capturing pipe, which is close to the counting component, is provided with a capturing pipe inlet, and the other end of the capturing pipe, which is far away from a technical component, is communicated with the waste liquid outlet; and the rear pool cleaning outlet is arranged at the middle part of the capturing pipe, and is respectively communicated with the rear pool and the waste liquid outlet. The invention also discloses a blood cell analysis device adopting the particle counting device. The particle counting device and blood cell analysis device disclosed by the invention have the advantages of simple structure and high reagent utilization ratio.

The invention discloses a measurement method and a measurement device capable of performing full-automatic peripheral blood sample injection for blood cell analysis. The measurement device comprises a test tube rack for loading a centrifuge tube, a sample injecting loading platform mechanism for sending the test tube rack into an analyzer, a sampling needle mechanism for spitting and sucking oscillation, an unloading retreating platform mechanism for retreating the test tube rack from the analyzer, an automatic diluent adding button, an automatic counting button and an automatic control system, wherein the automatic control system is connected with the sampling needle mechanism for spitting and sucking oscillation, the sample injecting loading platform mechanism, the unloading exiting platform mechanism, the automatic diluent adding button and the automatic counting button. After adoption of the measurement device and the measurement method, high automation degree and high efficiency can be achieved.

The invention discloses an automatic pipeline for blood cell analysis and POCT immunoassay, and the pipeline comprises a rack, a first conveying platform, a first mixing device, a first sampling mechanism, a first detector with an analyzing device, a first unloading platform, a first conveying device, a second conveying platform, a relay mechanism, a second mixing device, a second sampling mechanism and a fluorescent immunoassay analyzer with a plurality of test channels, wherein the automatic pipeline for blood cell analysis and POCT immunoassay with the above structure organically combines blood cell analysis and fluorescence immunoassay to achieve detection of blood cells and POCT immunization programs, and the plurality of test channels using the fluorescent immunoassay analyzer can freely combine different test items, thereby providing versatility and expandability, shortening sample detection cycle, improving efficiency, reducing manual intervention and reducing error rates.

The invention relates to hemolysin used for blood cell analysis, in particular to the environmental-protection typed hemolysin of no-cyanogen leucocyte three-classification used for blood cell analysis. The hemolysin comprises the components as follows (by density): 0.5-5.0g/L of sodium lauryl sulphate (SLS), 3.0-10.0g/L of quaternary ammonium salt, 0.5-5.0g/L of nonionic surfactant, 0.5-3.0g/L of alkali metal salt, and residual quantity of deionized water. The hemolysin of the invention can quickly dissolve erythrocyte and release haemoglobin; the hemolysin and the haemoglobin form stable haemoglobin derivatives so as to lead the concentration to be exact and easy to be measured; furthermore, the leucocyte three-classification measurement is carried out simultaneously. The hemolysin of the invention can be highly relative to the HiCN mensuration recommended by International Council for Standardization in Hematology (ICSH) and can carry out the leucocyte three-classification exactly and quickly.

The invention discloses a reagent set for a five-classification blood cell analyzer, which consists of a diluent, a hemolytic agent and a staining solution. The reagent set for the five-classification blood cell analyzer of the present invention has the advantages of reasonable formula, easy-to-obtain raw materials, easy processing, and low cost. Therefore, it has a good value for popularization and use; it changes the current situation that the domestic reagents for blood cell analyzers rely on imports for a long time, and The hemolytic agent-SLS in the reagent is a cyanide-free hemolytic agent, which greatly reduces the damage to the health of the production unit personnel and experiment operators, and also avoids the pollution of the environment caused by the use of cyanide. The reagent is suitable for the XN series five-differentiation automatic blood cell analyzer of Sysmex Corporation in Japan. The correlation coefficients between white blood cells, red blood cells, hemoglobin, platelets, etc. and imported XN reagents are all greater than 0.95.