158 results about "Blood cell analysis" patented technology

The present application provides a test kit and a POCT blood cell analyzer. The test kit includes a box body, a sealing ring, a microporous sheet, and a back pool body. The box body includes a front pool and a mounting cavity connected to the front pool; the sealing ring is arranged in the mounting cavity and is located on the side of the mounting cavity close to the front pool; the microporous sheet is provided with micropores that allow cells to pass through one by one; the microporous sheet is arranged in the mounting cavity and is located on the side of the sealing ring away from the front pool; the back pool body abuts against the side of the microporous sheet away from the sealing ring, and is used to fix the microporous sheet and the sealing ring on the box body; the back pool body is formed with a drainage cavity, and the front pool and the drainage cavity are connected through micropores. The test kit provided by the present application has a simple structure, is easy to assemble, and is conducive to the processing of the test kit.

The invention discloses a hematology analyzer and a bubble discharging method. The hematology analyzer comprises a flowing chamber, a pressure source, a first control valve, a second control valve and a third control valve, the flowing chamber comprises a liquid containing cavity, a first liquid inlet, a second liquid inlet and a liquid outlet, the first liquid inlet, the second liquid inlet and the liquid outlet are communicated with the liquid containing cavity, the first control valve is connected with the first liquid inlet through a pipeline, and the second control valve is connected with the liquid outlet through a pipeline. The third control valve is connected with the second liquid inlet through a pipeline, and the pressure source is connected with at least one of the first liquid inlet and the second liquid inlet through a pipeline; when the first liquid exists in the liquid containing cavity, the pressure source is matched with the first control valve, the second control valve and the third control valve to provide positive pressure and negative pressure for the liquid containing cavity, the liquid containing cavity is filled with liquid, second liquid is obtained, and bubbles in the second liquid are smaller than bubbles in the first liquid. Therefore, bubbles of the liquid in the flow chamber can be discharged through a simple structure.

A blood cell analyzer (100) and a blood cell analysis method (300). The blood cell analysis method (300) comprises: sucking a blood sample to be tested; preparing a first determination sample and obtaining first optical information; preparing a second determination sample and obtaining second optical information; generating a first leukocyte classification scatter diagram and a second leukocyte classification scatter diagram; determining a first counting result of neutrophils of the to-be-detected blood sample based on the first leukocyte classification scatter diagram and the second leukocyte classification scatter diagram; determining an actual distribution area of neutrophils from the first leukocyte classification scatter diagram; based on a reference distribution region of neutrophils of a reference leukocyte classification scatter diagram of the normal blood sample, determining a leaf-division nucleophore distribution region from the actual distribution region; determining a second counting result of the cells falling into the leaf-separating nucleogranulocyte distribution area; and based on the first counting result and the second counting result, determining a counting result of the rod-shaped nuclear granulocytes in the to-be-detected blood sample.

The invention relates to the technical field of blood cell analysis, and particularly discloses a blood cell analysis system and method based on computer vision, which uses an image recognition and generation technology based on computer vision to carry out grid division and semantic feature extraction on blood image data. Performing global text generation and semantic coding on each blood image data grid block feature after semantic extraction so as to perform adjustment response aggregation on attention of different blood local areas in each blood image data grid block feature according to blood image text description semantic features; therefore, intelligent optimization of the blood image data is realized. In this way, the complex deep-layer mutual influence association relationship between cells can be captured, so that a more accurate diagnosis basis is provided. Meanwhile, the processing strategy can be dynamically adjusted according to the specific description content of the blood image so as to better adapt to diversified samples, and the method does not only depend on fixed preprocessing parameters.

The invention discloses a portable blood cell analysis method based on a micro-fluidic technology, which comprises the following steps: preprocessing a collected blood sample, adding the preprocessed blood sample into a micro-fluidic chip, turning on a light source while the sample flows through an image acquisition area, and shooting the sample in real time by using an image sensor to obtain a blood cell image; the method comprises the following steps: carrying out graying, denoising, contrast enhancement and other preprocessing operations on collected images, segmenting cells and backgrounds through a self-adaptive threshold method, and carrying out differential calculation according to motion characteristics of the cells in a system and adjacent three frames of images to finish dynamic capture of target cells, so as to obtain cell image data sets of different postures in a camera view. A spatial pyramid pooling structure SPPF in a YOLOv8 model is replaced by SPPCSPC, a GAM attention mechanism is introduced into a backbone network, sample images are labeled in batches by using a labmeli labeling tool to obtain final sample data sets of different types of cells, and the improved YOLOv8 model is trained to realize classification and counting of the cells.

The invention discloses a sample detection device applied to instant detection, a bearing assembly of the sample detection device comprises a first loading seat and a second loading seat, the first loading seat is used for bearing a kit and a to-be-detected sample, or the first loading seat is used for bearing the kit, and the to-be-detected sample is stored in the kit; the second loading seat is used for bearing a reagent card, and the reagent card is provided with a sample adding area. The pipetting assembly is used for acquiring the to-be-detected sample on the first loading seat and transferring the to-be-detected sample to the reagent card for sample adding, so that automatic sample adding of the reagent card is realized, the operation is simple, manual intervention can be effectively reduced, and the detection efficiency of the hematology analyzer is improved.

The utility model belongs to the technical field of medical instruments, and particularly relates to a blood extraction device for a hematology analyzer, which comprises an electric telescopic frame and a detection module, the electric telescopic frame is positioned on one side of a hematology analyzer main body and is connected with a base, a plurality of clamping grooves I are formed in the electric telescopic frame, and the detection module is connected with the electric telescopic frame. An extraction needle cylinder is detachably installed in the first clamping groove, an extraction needle head is arranged at one end of the extraction needle cylinder, a piston is movably connected to the interior of the extraction needle cylinder in a sealed mode, the detection cylinder is driven to descend through stretching and retracting of the electric telescopic frame, and in the descending process of the detection cylinder, after the detection module detects the layering position of plasma and blood cells, the extraction needle head is connected to the extraction needle cylinder; the controller controls the electric telescopic frame to move, at the moment, the push rod is pulled, the extraction needle head is just located above the layering position, the plasma is extracted into the needle cylinder, one-time plasma extraction is achieved through accurate detection of the detection module, and waste of the plasma is reduced as much as possible.

The invention discloses a hematology analyzer and a platelet numerical value measuring method, in the hematology analyzer, an analysis unit is used for receiving a pulse signal to generate a first image, receiving an optical signal to generate a second image, obtaining a platelet original numerical value based on the first image, obtaining an interference coefficient of a leukocyte fragment based on the second image, and obtaining the platelet numerical value based on the interference coefficient; and obtaining a platelet measurement value based on the platelet original value and the interference coefficient. Through the cooperation of the impedance module and the flow fluorescence module, the platelet original value in the blood cell sample and the interference coefficient of the leukocyte fragment are detected, and the accuracy of each step in the calculation process of the platelet measurement value is ensured. Meanwhile, the platelet measurement numerical value is calculated through the analysis unit based on the platelet original numerical value and the interference coefficient, so that the accuracy of the blood cell analyzer for measuring the platelet measurement numerical value is improved, the practicability of the blood cell analyzer is improved, and the use experience of a user on the blood cell analyzer is improved.

The invention discloses a hematology analyzer and a detection method for hole blockage of a flow chamber, in the hematology analyzer provided by the invention, in the process that sample liquid enters the flow chamber for sample detection, a control unit can also obtain signal parameters based on optical signals of the sample liquid flowing through the flow chamber; whether the flow chamber is blocked or not is judged, the safety of the flow chamber is improved, and the detection efficiency of an optical detection system on sample liquid is improved. Meanwhile, in the process of detecting the sample liquid, whether the flow chamber has the hole blocking problem or not can be judged, the step of judging the hole blocking of the flow chamber does not need to be additionally set, the operation efficiency of the hematology analyzer is improved, and the use experience of a user on the hematology analyzer is improved.

The present application provides a blood cell classification method, in which the impedance pulse signal of the blood sample to be classified is input into a trained target neural network classification model. The target neural network classification model is trained by a large number of impedance pulse signal samples with classification result label information. It has a strong pulse morphology feature extraction capability, so that the classification result of the impedance pulse signal can be determined based on the morphological characteristics of the impedance pulse signal of the blood sample to be classified. Compared with the existing technology, the classification result is more accurate, and it can identify the impedance pulse signal caused by interference factors, and can perform identification and classification based on the impedance pulse signal, with higher real-time performance. In addition, the present application also provides a blood cell analyzer and a storage medium.

The invention discloses a POCT hematology analyzer, a kit, a detection assembly and a detection method.The detection assembly comprises a detection part, a first electrode and a second electrode, the detection part is provided with a liquid storage cavity and a liquid drainage cavity, the liquid storage cavity is used for storing a first to-be-detected sample, the bottom of the detection part is provided with micropores, and the micropores are used for communicating the liquid storage cavity with the liquid drainage cavity; the detection assembly is installed on the sample analyzer, and the sample analyzer sucks first to-be-detected samples through the liquid discharge cavity and counts the first to-be-detected samples through the first electrode and the second electrode. Therefore, the sample analyzer does not need to clean the detection piece, a cleaning system is saved, the cost is reduced, and the detection efficiency is improved.

The utility model relates to the technical field of hematology analyzers, in particular to a hematology analyzer probe cleaning mechanism which can clean the outside and the inside of a probe, improve the cleaning effect, recycle cleaning fluid and reduce waste. Comprising a cleaning cylinder, an inserting assembly, a cleaning assembly and a recycling assembly, an inserting opening is formed in the top end of the cleaning cylinder, the inserting assembly is installed at the position of the inserting opening in the top end of the cleaning cylinder, the cleaning assembly is installed at the bottom end of the cleaning cylinder, and the output end of the cleaning assembly is located in the cleaning cylinder; the cleaning assembly comprises a liquid storage box, a plurality of fixing frames, a liquid adding pipe, a sealing cover, a water pump, a liquid pumping pipe, a conveying pipe, a plurality of connecting rods and a liquid storage disc, and the recycling assembly is installed on the right portion of the cleaning assembly.

The present disclosure relates to a method for white blood cell differential counting using whole blood in a fluid state, and the like. The method of the present disclosure does not include the drying and washing processes used in traditional blood cell analysis to provide a standard for multiple white blood cell analysis, thereby enabling rapid analysis with high repeatability and reproducibility even when large quantities of samples are being processed, and providing accurate test results including the numbers, ratios, and morphological discrimination for each type of white blood cells.

The application discloses a blood cell analyzer and a processing method of a red blood cell sample. In the blood cell analyzer, a control module grades the agglomeration degree of the red blood cell sample, and then processes the red blood cell sample according to the corresponding grade based on the agglomeration degree grade of the red blood cell sample, to obtain a detection result of the red blood cell sample. The control module can intelligently grade the agglomeration degree of the red blood cell sample, and process the red blood cell sample according to the corresponding grade based on the agglomeration degree grade of the red blood cell sample, so that the blood cell analyzer can automatically process the agglutinated red blood cell sample, the agglomeration effect of the red blood cell sample is reduced, the detection accuracy of the blood cell analyzer for the red blood cell sample is improved, the probability of missing detection in the manual report auditing process is reduced, the workload of manual disposal is reduced, time for accurately and quickly issuing a detection report is saved, and the user experience of the blood cell analyzer is improved.

The invention discloses a POCT (Point of Care Testing) hematology analyzer. In the application, the first bearing position is arranged on the sample injection module to bear the detection card for receiving the first to-be-detected sample, and the second bearing position is arranged on the first detection module to bear the detection card after receiving the first to-be-detected sample, so that the receiving and detection of the first to-be-detected sample are separately carried out at different bearing positions of different modules; the transfer module is arranged at the output end of the driving module of the preprocessing module, so that the driving module can drive the transfer module to transfer the detection card from the first bearing position to the second bearing position after driving the pipetting consumable through the loading module to transfer the first to-be-detected sample into the detection card at the first bearing position; therefore, the driving module is ingeniously used for driving the transfer module, and a driving device special for driving the transfer module is avoided, so that the overall structure of the analyzer is simpler, the production cost is reduced, and the analyzer is more compact and miniaturized in structure.

The invention provides a fluorescent dye for blood cell analysis. The fluorescent dye has the following structural general formula I, wherein X is CH2, C (CH3) 2, O, S or Se; y is-CH2-CH2 = CH-, or is selected from any one of the following structural formulas II, III and IV, and Z is an anion; r1 and R2 are respectively and independently selected from straight-chain or branched-chain C1-10 alkyl, benzyl and-(CH2) mNR3R4, the benzyl is optionally substituted by the following substituent groups: halogen, hydroxyl, sulfydryl and alkyl, and R1 and R2 are not benzyl at the same time; m is 1-10, and R3 and R4 are independently selected from H and straight-chain or branched-chain C1-6 alkyl groups respectively.

The purpose of the present invention is to provide a means for making it possible to sort and count reticulocytes and platelets. The blood cells in a test specimen are stained with a fluorescent dye, fluorescence information, first scattered light information, and second scattered light information that are generated by irradiating the stained blood cells with light having a wavelength of from 315 nm to 600 nm and light having a wavelength of from 610 nm to 750 nm are acquired, and the fluorescence information, the first scattered light information, and the second scattered light information are acquired on the basis of the first scattered light information and the second scattered light information, and / or on the basis of the first scattered light information and the second scattered light information, and / or on the basis of the first scattered light information and the second scattered light information. The problem is solved by specifying blood cells other than leukocytes on the basis of the first scattered light information and the fluorescence information, and classifying reticulocytes and platelets from groups other than leukocytes on the basis of the fluorescence information and the second scattered light information.

The hematology analyzer comprises a main machine and a puncture device, a front face cover is fixedly arranged at the front end of the main machine, a sampling groove of a concave structure is formed in the edge of the lower end portion of the front face cover, and the puncture device is fixedly arranged in the upper end portion of the front face cover. A sampling needle of the puncture device can vertically and downwards extend out of the front mask into the sampling groove; the control end of the puncture device is electrically connected with the host; a sampling key or a puncture test tube adapter is arranged on the inner wall of the sampling groove, and the sampling key or the puncture test tube adapter is electrically connected with the host. Compared with the prior art, the utility model realizes the switching between an automatic sampling mode and a manual sampling mode, and has the advantages of flexible conversion, stronger independence, convenience in installation and adjustment, efficient and convenient sampling and low cost; and operation is convenient, size is small, parts are few, and maintenance is convenient.

Disclosed is a method for analyzing blood cells, the method including: acquiring first scattered light information, second scattered light information, and fluorescence information generated by irradiating a measurement sample that contains a particle stained with a fluorescent dye with a light of a first wavelength and a light of a second wavelength; identifying blood cells other than white blood cell from the particles in the measurement sample, with reference to the first scattered light information and the second scattered light information, and / or, with reference to the first scattered light information and the fluorescence information; and classifying the blood cells other than white blood cell into reticulocyte and platelet, with reference to the fluorescence information and the second scattered light information, the first wavelength being 315 nm or longer and 600 nm or shorter, and the second wavelength being 610 nm or longer and 750 nm or shorter.

This application provides a method for using a POCT hematology analyzer, comprising: in response to a reagent kit being placed in a test chamber, controlling a pipette to move above the first mounting head reservoir of the reagent kit and descending to load the first mounting head; controlling the pipette to move to the diluent reservoir of the reagent kit to transfer the diluent into the pre-cell for impedance detection; controlling the pipette to transfer a sample into the pre-cell; mixing the test solution in the pre-cell; controlling the metal shielding cap to close with the test chamber to establish a connection between the rear cell and the pressure building system of the reagent kit; controlling the pressure building system to guide the test solution from the pre-cell into the rear cell; and detecting the test solution by impedance detection.

To reduce a work load by shortening a moving distance of an operator.SOLUTION: A composite analysis system 1 includes: a blood cell analyzer 2; a blood coagulation measurement device 3; and a transport device 4 having a transport path 50. The blood cell analyzer 2, the blood coagulation measurement device 3, and the transport device 4 are arranged at a position and orientation that allow at least one of access to a power supply operation reception unit of the blood cell analyzer 2 and the blood coagulation measurement device 3 from one side along the transport path 50, access to a consumable item setting unit of the respective devices from one side along the transport path 50, access to a terminal device of the respective devices from one side along the transport path 50, and access to a maintenance management target unit of the respective devices from one side along the transport path 50.SELECTED DRAWING: Figure 1

The invention discloses a POCT blood cell analyzer, a control method thereof and a kit assembly. The POCT hematology analyzer comprises an acquisition unit, a detection unit and a processing unit. Wherein the acquisition unit is used for acquiring a calibration coefficient corresponding to the disposable kit, the kit comprises an impedance detection cell, and a microporous sheet is arranged in the impedance detection cell; the detection unit is used for being connected with the impedance detection pool and controlling a sample in the impedance detection pool to pass through the microporous sheet so as to detect a preset item of the sample; and the processing unit is used for obtaining a final detection value of the sample based on the detection result of the sample and the calibration coefficient. In the application, the POCT hematology analyzer corrects the detection result of the kit through the calibration coefficient of the kit, so that the accuracy of sample detection is improved.

The application discloses a blood cell analyzer and a blood cell analysis method. The liquid storage assembly of the blood cell analyzer comprises a liquid storage cavity. The height of the inlet of the liquid storage cavity is less than the height of the outlet. The first end of the temporary storage mechanism of the liquid path emptying assembly is connected with the pressure supply mechanism. The second end of the temporary storage mechanism is connected with the inlet of the liquid storage assembly through the first control valve. The processor is used to control the pressure supply mechanism to switch the temporary storage mechanism to the negative pressure state in response to receiving the liquid draining instruction. The processor is also used to control the first control valve to conduct the liquid storage assembly and the temporary storage mechanism, so that the liquid in the liquid storage cavity is drained through the pipeline between the first control valve and the liquid storage assembly. The liquid in the liquid storage cavity can be drained through the pipeline between the first control valve and the liquid storage assembly by the temporary storage mechanism in the negative pressure state, the residual reagent in the liquid storage cavity is removed, the blockage failure caused by the evaporation and crystallization of the reagent is reduced or avoided, and the service life and reliability of the blood cell analyzer are improved.

The utility model discloses a built-in hemolytic agent bottle and hematology analyzer relates to medical instrument technical field, wherein the built-in hemolytic agent bottle includes solvent bottle, movable joint block and pipeline subassembly, solvent bottle includes mutually communicated bottle body and bottle cap, bottle body and bottle cap detachable connection, bottle cap far away from bottle body side is equipped with the movable mouth; the movable connector block is movably arranged at the movable opening, the movable connector block and the movable opening are arranged in a clearance mode, and the movable connector block is provided with a mounting hole which is formed in the thickness direction in a penetrating mode; the pipeline assembly is fixedly arranged at the mounting hole, one end of the pipeline assembly extends into the bottle body, and the other end of the pipeline assembly penetrates out of the mounting hole and is communicated with an external liquid system. According to the technical scheme, the problem that when a bottle cap of an existing built-in hemolytic agent bottle is screwed down, a pipeline is prone to being bent is solved.

The invention belongs to the technical field of biomedical detection, and discloses a blood cell analysis system based on image flow cytometry, which comprises a cell staining module, the dyeing module simultaneously comprises a first fluorescent probe capable of being specifically combined with components in a cell nucleus or enriched in the cell nucleus, a second fluorescent probe capable of being specifically combined with cell nucleus DNA and a third fluorescent probe capable of being specifically combined with a blood cell differentiation antigen; an image flow cytometer; and the image processing module is used for calculating the kernel detection rate and / or the average kernel number. According to the present invention, the combination of the specific types of the cell staining dyes is adopted, the image flow cytometry is matched, and the optimized multi-dimensional fluorescence staining combination and the matched automatic image analysis process are adopted to achieve the synchronous, high-throughput and accurate quantitative analysis of the blood cell immune phenotype and the cell morphology key characteristics at the single cell level;

The invention relates to the technical field of animal blood cell detection and analysis, in particular to a method for an animal blood cell analyzer to recognize platelet aggregation, which comprises the following steps: after the animal blood cell analyzer performs pretreatment and fluorescent staining on a blood sample in a white blood cell DIFF channel, acquiring three signals of blood cells through laser flow cytometry; two scatter diagrams are drawn according to the three signals, platelet aggregation particles are identified in a certain area range in the two scatter diagrams, when the number exceeds a threshold value, an alarm is given, and normal leukocytes cannot fall in the area. The good alarm function of the instrument provides more accurate prompts for clinical diagnosis, and clinical appropriate microscopic examination of platelets is facilitated. The cost is saved, extra testing is not needed, and the alarm function can be achieved in the leukocyte DIFF channel in the blood routine testing mode. The platelet aggregation region is identified, the influence of platelet aggregation on the total number of leukocytes is eliminated, and the leukocyte value is more accurate.

A blood cell analyzer (100) and a blood cell analysis method (300). The blood cell analysis method (300) comprises: aspirating a blood sample to be tested; preparing a first assay sample, and obtaining first optical information; preparing a second assay sample, and obtaining second optical information; generating a first white blood cell classification scattergram and a second white blood cell classification scattergram; based on the first white blood cell classification scattergram and the second white blood cell classification scattergram, determining a first count result of neutrophils in the blood sample to be tested; determining an actual distribution region of neutrophils from the first white blood cell classification scattergram; based on a reference neutrophil distribution region of a reference white blood cell classification scattergram of a normal blood sample, determining a segmented granulocyte distribution region from the actual distribution region; determining a second count result of cells falling within the segmented granulocyte distribution region; based on the first count result and the second count result, determining a count result of band granulocytes in the blood sample to be tested.

This application relates to a sample analysis system and a method for detecting platelet abnormalities. The sample analysis system includes: a blood cell analysis device, comprising a sample preparation unit and a detection unit, wherein the sample preparation unit is configured to prepare a test sample from a blood sample to be tested, and the detection unit is configured to test the test sample to obtain a first platelet detection result; a smear preparation device, configured to prepare a smear of the test sample from the blood sample to be tested; a cell image capturing device, configured to capture cell images of the test sample smear to obtain cell images of the blood sample to be tested, and to analyze the cell images to obtain a second platelet detection result; and a data processing device, configured to determine whether platelet abnormalities exist in the blood sample to be tested based on the first and second platelet detection results, and to output a platelet abnormality prompt when a platelet abnormality is determined to exist. This enables more accurate identification of platelet abnormalities.

Provided are a test specimen analysis method and a test specimen analysis device that support rapid evaluation of the severity of an organ disorder caused by an infectious disease, and a test specimen analysis method and a test specimen analysis device that are capable of outputting information highly related to a score indicating the severity of an organ disorder caused by an infectious disease. The blood cell analyzer (1) includes a measurement unit (measurement unit (2)) that irradiates a measurement sample containing a blood specimen and a fluorescent dye for staining leukocytes contained in the blood specimen with light, detects fluorescence of the leukocytes contained in the measurement sample irradiated with the light, and measures a fluorescence signal on the basis of the detected fluorescence. The method comprises the following steps: generating information related to the distribution width of the fluorescence intensity of a neutrophil group contained in a measurement sample, and generating information related to the distribution width of the fluorescence intensity of the neutrophil group based on the information related to the distribution width of the fluorescence intensity of the neutrophil group; an information processing unit (main body 40) that specifies severity-related information of the organ disorder caused by the infectious disease; and an output unit 42 that outputs the severity-related information of the organ disorder caused by the infectious disease.

The invention discloses an intelligent blood sample identifying and sorting system, and relates to the technical field of sorting. The system comprises a sorting central control management server, a composite robot unit, a sample receiving unit, a sample identifying and sorting unit, a recovery processing unit, a blood cell analyzer, a blood clotting instrument, a serum analyzer and a plasma analyzer, the sorting central control management server is respectively connected and communicated with the composite robot unit, the sample receiving unit, the sample identifying and sorting unit and the recovery processing unit; the sorting central control management server, the hematology analyzer, the blood clotting instrument, the serum analyzer and the plasma analyzer are all used for being connected and communicated with a hospital clinical laboratory information system LIS; through the arrangement of distributed systems such as the sorting central control management server, the composite robot unit, the sample receiving unit, the sample identifying and sorting unit, the recovery processing unit, the hematology analyzer, the blood clotting instrument, the serum analyzer and the plasma analyzer, the efficiency is improved, and the occupied area is saved.