33 results about "Functional identification" patented technology

The invention relates to a trigger signal identification method and device, equipment and a storage medium. The trigger signal identification method comprises the following steps: acquiring an FPGA design code to be subjected to trigger signal identification; traversing at least one module code in the FPGA design codes, and extracting trigger signal features from block statements in the module codes and / or a target list; wherein the target list is composed of at least one field corresponding to the preset identifier, and the at least one field is used for representing the trigger signal information; performing function identification on the trigger signal features, and determining at least one trigger signal; and generating a trigger signal identification result according to the at least one trigger signal and the reference name of the at least one trigger signal in the corresponding module code. According to the method provided by the invention, the accuracy of trigger signal identification is improved, and the adaptability to complex design scenes is higher.

1. The name of the design product: single-lens multifunctional identification chip. 2. The use of the design product: biometric identification and cat eye visibility, monitoring function. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The application belongs to the technical field of genetic engineering, and particularly relates to application of NtNRAMP2 protein, a method for improving tobacco cadmium stress resistance and a method for obtaining tobacco plants with cadmium stress resistance. The application provides application of NtNRAMP2 protein in creating tobacco with cadmium stress resistance or breeding tobacco with cadmium stress resistance, and an amino acid sequence of the NtNRAMP2 protein is shown as SEQ ID NO:2. The NtNRAMP2 protein is synthesized by NtNRAMP2 gene, and it is proved by transgenic and functional identification that the NtNRAMP2 natural resistance macrophage protein synthesized by the NtNRAMP2 gene is closely related to tobacco cadmium absorption regulation.

The invention provides a method for improving the VIGS research efficiency of solanaceous vegetables and application of the method, and belongs to the technical field of biology. Specifically, a UP gene interference fragment and a target gene interference fragment are connected in series, double-gene silencing is utilized, UP is used as a reporter gene, and whether the target gene is silenced or not is judged according to orientation changes of flowers and fruits of a plant before and after UP gene silencing, so that gene silencing judgment is more visual and efficient; the method can greatly improve the screening efficiency of functional genes, has important significance in accelerating screening and functional identification of solanaceous vegetable genes, and particularly has wide application prospects in exploration and functional identification of control genes related to flower and fruit development, fruit ripening and metabolism.

The application relates to the technical field of protein-related data processing, in particular to a biological information processing system and method applied to synthetic biology. The method comprises the following steps: acquiring real-time proteomics data, performing polymorphic context decoding, and obtaining a protein expression context matrix; analyzing the interaction of behavior characteristics in the protein expression context matrix, and obtaining a multi-scale causal structure graph; performing structure-function transformation rule extraction on the multi-scale causal structure graph, and obtaining a function mapping unit set; evaluating the function mapping unit set, thereby forming a configuration decision graph; simulating the path in the configuration decision graph, performing path adaptability scoring, and obtaining a path adaptability feedback table; and optimizing the path adaptability feedback table, thereby obtaining an optimal expression configuration set. The application helps to improve the accuracy, stability and controllability of protein information in the structural analysis, function identification and regulation path construction process.

The invention relates to the technical field of animal blood cell detection and analysis, in particular to a method for an animal blood cell analyzer to recognize platelet aggregation, which comprises the following steps: after the animal blood cell analyzer performs pretreatment and fluorescent staining on a blood sample in a white blood cell DIFF channel, acquiring three signals of blood cells through laser flow cytometry; two scatter diagrams are drawn according to the three signals, platelet aggregation particles are identified in a certain area range in the two scatter diagrams, when the number exceeds a threshold value, an alarm is given, and normal leukocytes cannot fall in the area. The good alarm function of the instrument provides more accurate prompts for clinical diagnosis, and clinical appropriate microscopic examination of platelets is facilitated. The cost is saved, extra testing is not needed, and the alarm function can be achieved in the leukocyte DIFF channel in the blood routine testing mode. The platelet aggregation region is identified, the influence of platelet aggregation on the total number of leukocytes is eliminated, and the leukocyte value is more accurate.

The application discloses a tobacco salt-tolerant gene Nt-zfhd-6 and application thereof. The nucleotide sequence of the tobacco salt-tolerant gene Nt-zfhd-6 comprises a sequence as shown in SEQ ID No. 1. The amino acid sequence coded by the tobacco salt-tolerant gene Nt-zfhd-6 is shown in SEQ ID No. 2. Under a salt stress condition, the expression amount of the Nt-zfhd-6 gene in tobacco seedling leaves is increased by nearly 40 times. The gene is amplified, enzyme-cut, and constructed into an expression vector, and then is infected into tobacco by means of agrobacterium, and a tobacco with the Nt-zfhd-6 gene is obtained. Under a salt stress treatment condition, the root length of the tobacco is obviously increased compared with that of a wild type, and it is proved that the Nt-zfhd-6 gene is a salt-tolerant gene. The functional identification of the tobacco Nt-zfhd-6 gene enriches the gene resources of tobacco salt-tolerant breeding, and has important theoretical significance and application value.

The invention discloses application of peanut exocrine peptide AhSIP1 in improving salt tolerance of plants, and belongs to the technical field of molecular breeding. The nucleotide sequence of the peanut exocrine peptide AhSIP1 is as shown in SEQ ID NO: 1, and the sequence of amino acid coded by the peanut exocrine peptide AhSIP1 is as shown in SEQ ID NO: 2. Experiments prove that when the AhSIP1 is mature and applied in vitro, peanut seed germination under salt stress can be remarkably promoted, 35S: AhSIP1 transgenic apple callus grows faster (fresh weight is larger) under salt stress, and it is indicated that the peanut exocrine small peptide AhSIP1 positively regulates plant salt tolerance. Functional identification of the peanut exocrine small peptide AhSIP1 provides theoretical basis and technical guidance for seedling emergence, full seedling and high-yield cultivation of saline-alkali soil plants, and has important application value in salt-tolerant crop breeding.

The invention discloses a method for synthesizing carnosic acid in vitro and application of the carnosic acid in improvement of oxidation resistance, and belongs to the field of molecular biology. According to the application disclosed by the invention, SrCYP716A514 and SrCYP76AK7 genes positioned in endoplasmic reticulum are found, the SrCYP716A514 and SrCYP76AK7 genes are found to be capable of synthesizing the carnosic acid in vitro, and the content of the carnosic acid is obviously increased when the SrCYP716A514 and SrCYP76AK7 genes are overexpressed in rosemary, and the oxidation resistance of a rosemary extract can be enhanced. Through separation and functional identification analysis of the SrCYP716A514 gene and the SrCYP76AK7 gene, it is determined that the SrCYP716A514 gene and the SrCYP76AK7 gene can synthesize carnosic acid in vitro, the content of the carnosic acid is remarkably increased through overexpression in rosemary, and the oxidation resistance of a rosemary extract can be enhanced. According to the invention, synthesis of carnosic acid in plants can be accurately regulated and controlled, the carnosic acid content can be increased, the method can be used for cultivating transgenic plants, plants with high carnosic acid content can be obtained, and the method has a wide application prospect in the fields of food, medicines, cosmetics and the like.

The invention relates to the technical field of cell differentiation degree evaluation, and particularly discloses a stem cell differentiation degree evaluation method based on electron microscope image recognition, which comprises the following steps: adjusting electron microscope imaging parameters of each stem cell differentiation sample; based on the preprocessed stem cell differentiation sample images, stem cell differentiation degree characteristic data is extracted and analyzed, stem cell differentiation influence factors are matched, the stem cell differentiation degree in the stem cell differentiation sample images is determined based on a convolutional neural network model, and a stem cell differentiation thermodynamic diagram is generated; and determining a culture parameter adjustment instruction at the time of each stem cell differentiation sample image. The problems that a traditional cell marker detection method is time-consuming, labor-intensive and high in cost, functional identification is limited to detection of a single cell, high-throughput analysis is difficult to achieve, efficiency is low, the requirement of large-scale research is difficult to meet, dynamic adaptive capacity is insufficient, and evaluation precision is insufficient are solved.

The invention discloses a dendrobium MYB transcription factor gene DoMYB59 and application of the dendrobium MYB transcription factor gene DoMYB59. The invention relates to application of a transcription factor DoMYB59 or a coding gene DoMYB59 thereof in regulation and control of synthesis of rutin in plants. The amino acid sequence of the transcription factor DoMYB59 is shown as SEQ ID NO.2. The invention also relates to application of the transcription factor DoMYB59 in regulation and control of synthesis of rutin in plants. A coding gene MYB transcription factor DoMYB59 belonging to a dendrobium MYB transcription factor DoMYB59 is cloned from dendrobium officinale leaves, the MYB transcription factor DoMYB59 is used as a positive regulation factor to participate in regulation of a rutin synthesis process of dendrobium, and transient expression and functional identification prove that the rutin content of transgenic dendrobium is increased when the MYB transcription factor DoMYB59 is excessively expressed. Therefore, the MYB transcription factor DoMYB59 or the coding gene thereof has important theoretical guidance significance and application value in the fields of secondary metabolite synthesis and medical application of orchid plants.

The invention discloses a robot acupoint identification system and method based on multi-physical field active disturbance and application. The method aims at solving the problems that traditional acupuncture point positioning depends on subjective experience, the automation degree is low, and positioning is not accurate only based on static physical characteristics. The robot comprises a vision-guided multi-degree-of-freedom mechanical arm; and the bionic end effector is mounted at the tail end of the mechanical arm. The core innovation of the actuator is that the actuator is integrated with a thermal-electric cooperative TENG sensing array (TE-TENG Array) and an active pressing unit. The TENG array can be switched between a sensing mode and an excitation mode; the actuator is further integrated with a miniature heat flow disturbance unit and a high-precision temperature sensor. The acupoints are defined and positioned by identifying extreme points of functional responses, the leap from static positioning to dynamic functional identification is achieved, and the accuracy and objectivity of automatic acupoint positioning are greatly improved.

The invention discloses a dendrobe small RNA (Ribonucleic Acid) miR421 and an application thereof. According to the application of the miR421 in regulation and control of synthesis of the plant polysaccharide, the mature sequence of the miR421 is as shown in SEQ ID NO. 1. According to the invention, miR421 belonging to dendrobium is cloned from dendrobium officinale leaves, the miR421 is used as a negative regulatory factor to participate in regulating the synthesis process of dendrobium polysaccharide, and transient expression and functional identification prove that the polysaccharide content of transgenic dendrobium is reduced when the miR421 is excessively expressed. Therefore, the miR421 has important theoretical guidance significance and application value in the fields of synthesis of secondary metabolites of orchid plants and medical application.

Provided herein are methods for predicting unobserved phenotypes and selecting genetic variant organisms for effective use in genetically improving non-human animal species. Also provided herein are systems for implementing such methods, as well as computer-readable storage media storing instructions for performing such methods.

The invention discloses application of an NtFATB gene in regulation and control of tobacco glandular hair development, and belongs to the technical field of plant genetic engineering. The NtFATB gene silent tobacco plant is cultivated by constructing an RNAi vector of the NtFATB gene, the tobacco leaf glandular hair density of the silent plant is observed, and analysis shows that the glandular hair density in the tobacco leaf is remarkably reduced. Therefore, the NtFATB gene is proved to be a key gene for regulating and controlling the development of the tobacco glandular hair. The gene is used in the field of scientific research and can be used for exploring a glandular hair development molecular regulation mechanism, identifying glandular hair development related genes, breeding key tobacco varieties for regulating glandular hair development and the like. The functional identification of the tobacco NtFATB gene not only enriches the gene resources of tobacco for regulating and controlling glandular hair development and quality breeding, but also is beneficial to analyzing the diversity function of the tobacco NtFATB gene, and has important theoretical significance and application value.

The invention discloses a key gene NtOS for controlling ocimene biosynthesis in tobacco and application of the key gene NtOS. The nucleotide sequence of the key gene NtOS for controlling ocimene biosynthesis in tobacco comprises a sequence as shown in SEQ ID No. 1, and the coded amino acid sequence comprises a sequence as shown in SEQ ID No. 2. The NtOS gene is transformed into tobacco through amplification, enzyme digestion, expression vector construction and agrobacterium infection, the ocimene release amount of an obtained NtOS gene transformed tobacco plant is remarkably increased, it is proved that the NtOS gene is a key gene for controlling ocimene biosynthesis, the function identification of the tobacco NtOS gene enriches the gene resources of tobacco quality breeding, and the tobacco NtOS gene has a wide application prospect. The method has important theoretical significance and application value.

A method comprising, requiring to use a machine learning, ML, feature based on using a ML Model appropriate for the ML feature; receiving an activation message in relation to at least one of an activation of one ML Model available and appropriate for the ML feature, the one ML Model associated with at least one Functionality, or an activation of one Functionality of at least one Functionality associated with one ML Model available and appropriate for the ML feature, wherein the activation message indicates that the one ML Model and / or the one Functionality is activated; based on the received activation message, starting to use the one ML Model; and reporting at least one of ML Model performance key performance indicators, KPIs, of the one ML Model, or Functionality performance KPIs of the one Functionality associated with the one ML Model.

This invention provides a method, apparatus, and device for identifying closely related web pages based on function and structure. The method includes: acquiring page information of two web pages to be identified; extracting functional features from the two web pages, inputting the functional feature vectors into a functional classification model to predict the functional labels of the two web pages; searching for a corresponding clustering model in a clustering model set based on the functional labels; inputting the structural feature vectors into the corresponding clustering model to predict the cluster to which each of the two web pages belongs, wherein the clusters and the functional labels constitute the functional and structural features of the web pages; and determining whether the two web pages are closely related pages based on their respective functions and structures. This invention obtains the function of a page through functional identification, and then identifies the structure through structural clustering based on the functional information, thereby effectively characterizing the implementation structure of web pages and improving the accuracy of identifying closely related web pages.

A system for producing a therapeutic oligomer includes a computing device configured to design a proposed therapeutic oligomer sequence, wherein designing further comprises generating a genomic library for an organism from a gene target, initiating a sequence identification function, identifying a genomic locus that the proposed therapeutic oligomer sequence is predicted to bond to as a function of an off-target sequence function, selecting the proposed therapeutic oligomer sequence as a function of the sequence identification function, the genomic locus, and a criterion element, and synthesize a therapeutic oligomer as a function of the proposed therapeutic oligomer sequence.

The invention discloses a caulis sinomenii methyltransferase and an application of the caulis sinomenii methyltransferase in synthesis of sinomenine. The amino acid sequence of the caulis sinomenii methyltransferase is as shown in SEQ ID NO: 2. According to the invention, a new methyltransferase SaOMT3 is identified from a medicinal plant caulis sinomenii for the first time, and it is found that the methyltransferase participates in biosynthesis of sinomenine. Functional identification on SaOMT3 can greatly promote the research on the biosynthesis path of sinomenine, provide sufficient raw material guarantee for the creation of new sinomenine drugs, and also provide reference for the research of other medicinal plants. Meanwhile, the caulis sinomenii methyltransferase SaOMT3 disclosed by the invention is good in substrate specificity, high in catalytic efficiency and very high in application value.

The application belongs to the technical field of genetic engineering, and particularly relates to application of a transcription factor MdWRKY71 or a MdWRKY71 gene in promoting anthocyanin accumulation in plants, wherein an amino acid sequence of the transcription factor MdWRKY71 is shown as SEQ ID NO:1, and a nucleotide sequence of the MdWRKY71 gene is shown as SEQ ID NO:2. The transcription factor MdWRKY71 is synthesized by the MdWRKY71 gene, and it is proved by transgenic and functional identification that the transcription factor MdWRKY71 synthesized by the MdWRKY71 gene as a new transcription factor for regulating anthocyanin accumulation in apples can positively regulate expression of key anthocyanin synthesis enzyme genes MdANS and MdDFR, and promote 5-amino levulinic acid (ALA)-induced anthocyanin accumulation in apples, and can play an important role in agricultural and forestry production and scientific research.

This invention relates to a method for antibody reprogramming and screening based on engineered cells and its applications. This method achieves efficient in vitro rearrangement and diversity generation of antibody genes in non-lymphocytes by constructing engineered human cells containing the RAG1 / 2 recombinase system and programmable recombination signal sequence (RSS) elements. Utilizing this invention, the V(D)J rearrangement mechanism of B cells can be simulated in engineered cells, allowing for the targeted introduction of insertions, deletions, and point mutations in the antibody complementarity-determining region (CDR), resulting in a structurally diverse antibody library. Combined with piggyBac transposase-mediated genome integration, mammalian cell surface display technology, and flow cytometry sorting, high-throughput antibody screening and functional identification are achieved. This invention is simple to operate, has high antibody gene rearrangement efficiency, and produces a rich diversity of antibody libraries, making it suitable for rapid optimization of antibody drugs and discovery of new antibodies.

The invention discloses a Pictet-Spengler enzyme capable of identifying alpha-keto acid and application of the Pictet-Spengler enzyme capable of identifying the alpha-keto acid. According to the invention, a Pictet-Spengler enzyme gene apksB is obtained by cloning from actinomycetes NRRL B-65536T, the nucleotide sequence of the Pictet-Spengler enzyme gene apksB is as shown in SEQ ID NO.1, the full length of the Pictet-Spengler enzyme gene apksB is 960 bp, the amino acid sequence of the Pictet-Spengler enzyme gene apksB encoded by the Pictet-Spengler enzyme gene apksB is as shown in SEQ ID NO.2, and the Pictet-Spengler enzyme gene apksB contains 319 amino acids in total; the method comprises the following steps: cloning a Pictet-Spengler enzyme gene apkslB, connecting the Pictet-Spengler enzyme gene apkslB with an expression vector pET28a (+), then converting the Pictet-Spengler enzyme gene apkslB into escherichia coli BL21 (DE3), culturing, inducing expression, and purifying to obtain the Pictet-Spengler enzyme ApkslB which is used as a catalyst to catalyze the reaction of L-tryptophan and alpha-ketoglutaric acid so as to produce the tetrahydro beta-carboline skeleton kitasetasic acid. Discovery and functional identification of the Pictet-Spengler enzyme ApKslB have practical guiding significance on large-scale production of alkaloids, and the Pictet-Spengler enzyme ApKslB can be applied to the fields of biology, medicine, fine chemical engineering and the like.

The invention belongs to the field of gene engineering, and particularly relates to a gene and a method for improving corn characters. According to the invention, molecular markers are continuously developed in a QTL interval in several years, recombinant single plants are screened from offspring for offspring test, characters of tens of thousands of recombinant single plants are measured to reduce a functional interval, and finally, the functional gene ZCN9 capable of influencing the content of tocopherol in corn is finely positioned; the ZCN9 gene and the homologous gene ZCN10 of the ZCN9 gene are subjected to gene knockout so as to perform more detailed functional identification, and corn plants after the ZCN9 and ZCN10 genes are knocked out can increase the content of tocopherol in grains and improve the characters such as the flowering period, the plant height, the ear height, the grain protein content and the grain yield.

The application provides a functional identification of a novel virulence factor of a cell and an application thereof. Based on the research on the novel virulence factor of the cell, the application further provides a polypeptide delivery system and an application thereof. The polypeptide delivery system comprises a protein complex and a signal peptide, the signal peptide comprises at least 20 amino acids at the N-terminus of a PagT1 protein or a homologous protein thereof, the signal peptide is used for guiding the polypeptide to be loaded into the protein complex, and guiding the polypeptide to be positioned to the nucleus of a eukaryotic cell. The research of the application provides an important basis for constructing a safe and efficient novel tumor treatment system, and provides an innovative solution for the treatment of cancer and other major diseases.

The invention discloses application of an NtMPK4 gene in cold resistance of tobacco, and belongs to the technical field of molecular biology. According to the application of the NtMPK4 gene disclosed by the invention in the cold resistance of the tobacco, the sequence of the NtMPK4 gene is as shown in SEQ ID NO. 1. The NtMPK4 gene negatively regulates the cold resistance of the tobacco, and the excavation and functional identification of the gene provide important gene resources and theoretical basis for low-temperature-resistant breeding of the tobacco.

This invention relates to a method for quantifying the relationship between morphological subtypes and biological functions of organoids, comprising the following steps: Step 1, organoid heterogeneity analysis: Several primary organoids are induced and cultured and morphologically differentiated to screen for organoid systems that are morphologically distinguishable among different organ types, and the morphological subtypes of these systems are observed; Step 2, external perturbation processing of organoids; Step 3, bright-field imaging and image preprocessing; Step 4, prediction of biological function parameters using a biological function identification model; Step 5, addition of organoid morphological labels to the perturbated bright-field image data; Step 6, construction of a correlation map: the scores of biological function parameters are normalized and divided into quartiles, then sequentially correlated with morphological subtypes to construct a correlation map between morphological subtypes and biological function parameter scores. This invention can reveal the relationship between morphological heterogeneity and biological function with high accuracy.

The application discloses a kind of for synthesizing β - terpene synthase CcTPS6 of eucalyptol and its encoding gene and its product β - the application of eucalyptol as anti-aging drug belongs to synthetic biology and natural medicine chemistry technical field. The application starts from Labiatae plant torch flower ( Colquhounia coccinea var. mollis ) Cloning and functional identification of a synthetic β - eucalyptol single-function terpene synthase CcTPS6 encoding gene, its nucleotide sequence is as shown in Seq ID No.2, after codon optimization and site-directed mutagenesis, connect with expression vector pCold-TF, construct into the recombinant plasmid capable of expressing in escherichia coli, again the recombinant plasmid is transformed into escherichia coli and is constructed into engineering cell, realizes the heterologous high-efficiency synthesis compound β - eucalyptol in escherichia coli. The gene engineering cell constructed by the application is safe and stable, and the production cycle is short, which shows its great value in application development. The CcTPS6 product provided by the application β - eucalyptol has anti-aging activity and can be applied in the preparation of anti-aging drugs.

The present application provides a thermostable reductive aminase, its preparation method and application. Specifically, the present application relates to a thermostable reductive aminase and its functional identification and in vitro directed evolution. The reductive aminase of the present application is a reductive aminase from bacteria (BaRedAm) and its mutants (including a reductive aminase mutant with significantly improved enantiomeric selectivity of product rasagiline). The present application also provides an in vitro reductive amination method, comprising the steps of: (i) in the presence of a thermostable reductive aminase BaRedAm, subjecting (S1) a ketone substrate or an aldehyde substrate to a reductive amination reaction with (S2) an amine substrate. The enzyme and mutant of the present application can be used for in vitro enzymatic synthesis of different products such as enantiomerically pure rasagiline.