48 results about "Functional identification" patented technology

The invention discloses application of an NtTCP7 gene in cold resistance of tobacco, and belongs to the technical field of molecular biology. According to the application of the NtTCP7 gene disclosed by the invention in the cold resistance of the tobacco, the sequence of the NtTCP7 gene is as shown in SEQ ID NO. 1. The NtTCP7 gene negatively regulates and controls the cold resistance of the tobacco, and the cold resistance of the tobacco is remarkably improved by knocking out the gene. Excavation and functional identification of the gene provide important gene resources and theoretical basis for low-temperature-resistant breeding of tobacco.

The invention relates to the technical field of computer-aided drug research and development, and provides an antibacterial peptide function recognition optimization method and system based on deep learning and LLM. Systematic innovation is performed in multiple links such as feature extraction, data generation and reasoning verification by introducing a fusion mechanism of a large language model and a deep learning model; and the accuracy and robustness of antibacterial peptide function prediction are obviously improved. On one hand, a strict feature extraction process and a structured cue word template are constructed, sequence information and physicochemical property indexes of the antibacterial peptide and a prediction result of a deep learning model can be effectively integrated, and the prediction result is reasonably corrected and optimized through the reasoning ability and knowledge verification mechanism of a large language model. The misjudgment risk of a traditional deep learning method under the condition of insufficient samples or incomplete features is effectively reduced, and the prediction accuracy and stability are remarkably improved.

The invention relates to the technical field of protein related data processing, in particular to a biological information processing system and method applied to synthetic biology. The method comprises the following steps: acquiring real-time proteomics data, and performing polymorphic context decoding to obtain a protein expression context matrix; analyzing interaction of behavior characteristics in the protein expression context matrix to obtain a multi-scale causal structure map; executing structure-function transformation rule extraction in the multi-scale causal structure atlas to obtain a function mapping unit set; performing evaluation based on the function mapping unit set so as to form a configuration decision diagram; simulating paths in the configuration decision diagram, and performing path adaptability scoring to obtain a path adaptability feedback table; and optimizing the path adaptability feedback table to obtain an optimal expression configuration set. According to the method, the precision, the stability and the controllability of protein information in the process of structural analysis, function recognition and regulation path construction can be improved.

The invention discloses cloning and functional identification of a liverwort flavone synthetase gene, and belongs to the technical field of gene engineering. According to the invention, one flavone synthase I (flavones synthase I, FNS I) is obtained through screening, and is named as MpFNS I; a prokaryotic expression system is utilized, an MpFNS I recombinant protein is obtained through purification, and the result of in-vitro enzyme activity identification shows that an MpFNS I enzyme substrate is relatively wide in selectivity, has a flavone synthetase function and also has a part of flavonol synthetase function. The liverwort flavonoid has a significant difference on affinity and catalytic efficiency of different substrates, the in-vivo function of the liverwort flavonoid is verified by utilizing heterologous expression of arabidopsis thaliana, experimental evidence is provided for comprehensively analyzing a biosynthetic pathway of the liverwort flavonoid, and meanwhile, a research target is provided for deeply researching a mechanism for synthesizing and responding to UV-B radiation of the liverwort flavonoid.

The invention provides functional identification and application of a novel cell virulence factor. Based on the research on the novel virulence factor of the cell, the invention also provides a polypeptide delivery system and application thereof. Wherein the polypeptide delivery system comprises a protein complex and a signal peptide, the signal peptide comprises at least 20 amino acids at the N-terminal of PagT1 protein or homologous protein thereof, and the signal peptide is used for guiding the polypeptide to be loaded into the protein complex and guiding the polypeptide to be positioned to the nucleus of eukaryotic cells. The research of the invention provides an important basis for constructing a safe and efficient novel tumor treatment system, and provides an innovative solution for treating serious diseases such as cancers.

The invention relates to a trigger signal identification method and device, equipment and a storage medium. The trigger signal identification method comprises the following steps: acquiring an FPGA design code to be subjected to trigger signal identification; traversing at least one module code in the FPGA design codes, and extracting trigger signal features from block statements in the module codes and / or a target list; wherein the target list is composed of at least one field corresponding to the preset identifier, and the at least one field is used for representing the trigger signal information; performing function identification on the trigger signal features, and determining at least one trigger signal; and generating a trigger signal identification result according to the at least one trigger signal and the reference name of the at least one trigger signal in the corresponding module code. According to the method provided by the invention, the accuracy of trigger signal identification is improved, and the adaptability to complex design scenes is higher.

1. The name of the design product: single-lens multifunctional identification chip. 2. The use of the design product: biometric identification and cat eye visibility, monitoring function. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The present invention discloses a new rice disease susceptibility gene OsBSG1 Its functional identification method and its application in breeding new rice varieties with broad-spectrum disease resistance, high quality and high yield. According to the rice genome annotation information, clone OsBSG1 The CDS sequence of this gene is 2580 bp long and encodes a protein of 859 amino acids. OsBSG1 The CRISPR / Cas9 mutants of the gene were used to identify resistance to rice blast and found to be broadly resistant to rice blast; their thousand-grain weight was increased and their quality was better. OsBSG1 The discovery of these genes provides valuable genetic resources and theoretical guidance for the breeding of new rice varieties with broad-spectrum resistance to rice blast and high yield and quality.

The invention discloses a method for inducing orchid gene silencing through viruses. According to the method provided by the invention, orchid buds are used as acceptor materials; the construction method comprises the following steps: constructing a silent vector containing 200-300bp target genes by taking Super-MV-pro60 as a basic vector, and introducing the silent vector into agrobacterium to obtain an engineering bacterium; and injecting the engineering bacterium liquid into the buds, and culturing. According to the method, efficient silencing of orchid genes can be achieved, the silencing success rate is 75% or above, the silencing efficiency is 60%-95%, and the method is good in stability and has a good silencing effect under different environmental conditions. The method provided by the invention provides a new technical means for orchid gene function identification, realizes efficient silencing of orchid genes, can be used for rapid verification of orchid flower color, flower fragrance, flower type and other gene functions, and has important significance for orchid function gene identification and cultivation of new orchid varieties through a molecular breeding technology.

The application belongs to the technical field of genetic engineering, and particularly relates to application of NtNRAMP2 protein, a method for improving tobacco cadmium stress resistance and a method for obtaining tobacco plants with cadmium stress resistance. The application provides application of NtNRAMP2 protein in creating tobacco with cadmium stress resistance or breeding tobacco with cadmium stress resistance, and an amino acid sequence of the NtNRAMP2 protein is shown as SEQ ID NO:2. The NtNRAMP2 protein is synthesized by NtNRAMP2 gene, and it is proved by transgenic and functional identification that the NtNRAMP2 natural resistance macrophage protein synthesized by the NtNRAMP2 gene is closely related to tobacco cadmium absorption regulation.

The invention provides a method for improving the VIGS research efficiency of solanaceous vegetables and application of the method, and belongs to the technical field of biology. Specifically, a UP gene interference fragment and a target gene interference fragment are connected in series, double-gene silencing is utilized, UP is used as a reporter gene, and whether the target gene is silenced or not is judged according to orientation changes of flowers and fruits of a plant before and after UP gene silencing, so that gene silencing judgment is more visual and efficient; the method can greatly improve the screening efficiency of functional genes, has important significance in accelerating screening and functional identification of solanaceous vegetable genes, and particularly has wide application prospects in exploration and functional identification of control genes related to flower and fruit development, fruit ripening and metabolism.

The invention provides a rice OsNPR3 gene and application of the rice OsNPR3 gene in resisting rice blast, aiming at the production problem of how to improve the resistance of rice to the rice blast. Overexpression of the OsNPR3 gene can improve the rice blast resistance, and knockout or mutation of the OsNPR3 gene can improve the rice blast sensitivity. The nucleotide sequence of the OsNPR3 gene is as shown in SEQ ID NO. 1. According to the invention, rice blast resistance function identification is carried out on the OsNPR3 gene, and the positive regulation effect of the OsNPR3 gene in rice blast resistance is found and proved for the first time; experiments prove that the resistance of the rice to the rice blast is weakened after the OsNPR3 gene is knocked out, and the resistance of the rice to the rice blast can be enhanced by up-regulated expression of the OsNPR3 gene, so that the OsNPR3 gene positively regulates the resistance of the rice to the rice blast. The results show that the OsNPR3 gene is an excellent candidate gene for creating transgenic rice with enhanced rice blast resistance and molecular crop design, and has important theoretical value and wide application prospect.

The application relates to the technical field of protein-related data processing, in particular to a biological information processing system and method applied to synthetic biology. The method comprises the following steps: acquiring real-time proteomics data, performing polymorphic context decoding, and obtaining a protein expression context matrix; analyzing the interaction of behavior characteristics in the protein expression context matrix, and obtaining a multi-scale causal structure graph; performing structure-function transformation rule extraction on the multi-scale causal structure graph, and obtaining a function mapping unit set; evaluating the function mapping unit set, thereby forming a configuration decision graph; simulating the path in the configuration decision graph, performing path adaptability scoring, and obtaining a path adaptability feedback table; and optimizing the path adaptability feedback table, thereby obtaining an optimal expression configuration set. The application helps to improve the accuracy, stability and controllability of protein information in the structural analysis, function identification and regulation path construction process.

The invention relates to the technical field of animal blood cell detection and analysis, in particular to a method for an animal blood cell analyzer to recognize platelet aggregation, which comprises the following steps: after the animal blood cell analyzer performs pretreatment and fluorescent staining on a blood sample in a white blood cell DIFF channel, acquiring three signals of blood cells through laser flow cytometry; two scatter diagrams are drawn according to the three signals, platelet aggregation particles are identified in a certain area range in the two scatter diagrams, when the number exceeds a threshold value, an alarm is given, and normal leukocytes cannot fall in the area. The good alarm function of the instrument provides more accurate prompts for clinical diagnosis, and clinical appropriate microscopic examination of platelets is facilitated. The cost is saved, extra testing is not needed, and the alarm function can be achieved in the leukocyte DIFF channel in the blood routine testing mode. The platelet aggregation region is identified, the influence of platelet aggregation on the total number of leukocytes is eliminated, and the leukocyte value is more accurate.

The application discloses a tobacco salt-tolerant gene Nt-zfhd-6 and application thereof. The nucleotide sequence of the tobacco salt-tolerant gene Nt-zfhd-6 comprises a sequence as shown in SEQ ID No. 1. The amino acid sequence coded by the tobacco salt-tolerant gene Nt-zfhd-6 is shown in SEQ ID No. 2. Under a salt stress condition, the expression amount of the Nt-zfhd-6 gene in tobacco seedling leaves is increased by nearly 40 times. The gene is amplified, enzyme-cut, and constructed into an expression vector, and then is infected into tobacco by means of agrobacterium, and a tobacco with the Nt-zfhd-6 gene is obtained. Under a salt stress treatment condition, the root length of the tobacco is obviously increased compared with that of a wild type, and it is proved that the Nt-zfhd-6 gene is a salt-tolerant gene. The functional identification of the tobacco Nt-zfhd-6 gene enriches the gene resources of tobacco salt-tolerant breeding, and has important theoretical significance and application value.

The invention discloses application of peanut exocrine peptide AhSIP1 in improving salt tolerance of plants, and belongs to the technical field of molecular breeding. The nucleotide sequence of the peanut exocrine peptide AhSIP1 is as shown in SEQ ID NO: 1, and the sequence of amino acid coded by the peanut exocrine peptide AhSIP1 is as shown in SEQ ID NO: 2. Experiments prove that when the AhSIP1 is mature and applied in vitro, peanut seed germination under salt stress can be remarkably promoted, 35S: AhSIP1 transgenic apple callus grows faster (fresh weight is larger) under salt stress, and it is indicated that the peanut exocrine small peptide AhSIP1 positively regulates plant salt tolerance. Functional identification of the peanut exocrine small peptide AhSIP1 provides theoretical basis and technical guidance for seedling emergence, full seedling and high-yield cultivation of saline-alkali soil plants, and has important application value in salt-tolerant crop breeding.

The invention discloses a method for synthesizing carnosic acid in vitro and application of the carnosic acid in improvement of oxidation resistance, and belongs to the field of molecular biology. According to the application disclosed by the invention, SrCYP716A514 and SrCYP76AK7 genes positioned in endoplasmic reticulum are found, the SrCYP716A514 and SrCYP76AK7 genes are found to be capable of synthesizing the carnosic acid in vitro, and the content of the carnosic acid is obviously increased when the SrCYP716A514 and SrCYP76AK7 genes are overexpressed in rosemary, and the oxidation resistance of a rosemary extract can be enhanced. Through separation and functional identification analysis of the SrCYP716A514 gene and the SrCYP76AK7 gene, it is determined that the SrCYP716A514 gene and the SrCYP76AK7 gene can synthesize carnosic acid in vitro, the content of the carnosic acid is remarkably increased through overexpression in rosemary, and the oxidation resistance of a rosemary extract can be enhanced. According to the invention, synthesis of carnosic acid in plants can be accurately regulated and controlled, the carnosic acid content can be increased, the method can be used for cultivating transgenic plants, plants with high carnosic acid content can be obtained, and the method has a wide application prospect in the fields of food, medicines, cosmetics and the like.

The invention relates to the technical field of cell differentiation degree evaluation, and particularly discloses a stem cell differentiation degree evaluation method based on electron microscope image recognition, which comprises the following steps: adjusting electron microscope imaging parameters of each stem cell differentiation sample; based on the preprocessed stem cell differentiation sample images, stem cell differentiation degree characteristic data is extracted and analyzed, stem cell differentiation influence factors are matched, the stem cell differentiation degree in the stem cell differentiation sample images is determined based on a convolutional neural network model, and a stem cell differentiation thermodynamic diagram is generated; and determining a culture parameter adjustment instruction at the time of each stem cell differentiation sample image. The problems that a traditional cell marker detection method is time-consuming, labor-intensive and high in cost, functional identification is limited to detection of a single cell, high-throughput analysis is difficult to achieve, efficiency is low, the requirement of large-scale research is difficult to meet, dynamic adaptive capacity is insufficient, and evaluation precision is insufficient are solved.

The invention discloses a dendrobium MYB transcription factor gene DoMYB59 and application of the dendrobium MYB transcription factor gene DoMYB59. The invention relates to application of a transcription factor DoMYB59 or a coding gene DoMYB59 thereof in regulation and control of synthesis of rutin in plants. The amino acid sequence of the transcription factor DoMYB59 is shown as SEQ ID NO.2. The invention also relates to application of the transcription factor DoMYB59 in regulation and control of synthesis of rutin in plants. A coding gene MYB transcription factor DoMYB59 belonging to a dendrobium MYB transcription factor DoMYB59 is cloned from dendrobium officinale leaves, the MYB transcription factor DoMYB59 is used as a positive regulation factor to participate in regulation of a rutin synthesis process of dendrobium, and transient expression and functional identification prove that the rutin content of transgenic dendrobium is increased when the MYB transcription factor DoMYB59 is excessively expressed. Therefore, the MYB transcription factor DoMYB59 or the coding gene thereof has important theoretical guidance significance and application value in the fields of secondary metabolite synthesis and medical application of orchid plants.

The invention provides a lung cancer organoid verification and function identification technological process which comprises the following steps: step 1, collecting organoid, and step 2, carrying out verification and function identification on the organoid in the step 1. Wherein the verification and function identification in the step 2 are as follows: evaluating a development state; evaluating tumor characteristics; evaluating physiological functions; biological functions; identifying genetic characteristics; evaluating an immune microenvironment; and evaluating the drug sensitivity. The invention relates to the technical field of biomedicine, and provides a lung cancer organoid verification and function identification process flow, which aims to improve the quality of an organoid model, limit the application range of the organoid and finally provide help for exerting correct clinical and scientific research application values according to the performance of the organoid.

The invention discloses a robot acupoint identification system and method based on multi-physical field active disturbance and application. The method aims at solving the problems that traditional acupuncture point positioning depends on subjective experience, the automation degree is low, and positioning is not accurate only based on static physical characteristics. The robot comprises a vision-guided multi-degree-of-freedom mechanical arm; and the bionic end effector is mounted at the tail end of the mechanical arm. The core innovation of the actuator is that the actuator is integrated with a thermal-electric cooperative TENG sensing array (TE-TENG Array) and an active pressing unit. The TENG array can be switched between a sensing mode and an excitation mode; the actuator is further integrated with a miniature heat flow disturbance unit and a high-precision temperature sensor. The acupoints are defined and positioned by identifying extreme points of functional responses, the leap from static positioning to dynamic functional identification is achieved, and the accuracy and objectivity of automatic acupoint positioning are greatly improved.

The invention discloses a dendrobe small RNA (Ribonucleic Acid) miR421 and an application thereof. According to the application of the miR421 in regulation and control of synthesis of the plant polysaccharide, the mature sequence of the miR421 is as shown in SEQ ID NO. 1. According to the invention, miR421 belonging to dendrobium is cloned from dendrobium officinale leaves, the miR421 is used as a negative regulatory factor to participate in regulating the synthesis process of dendrobium polysaccharide, and transient expression and functional identification prove that the polysaccharide content of transgenic dendrobium is reduced when the miR421 is excessively expressed. Therefore, the miR421 has important theoretical guidance significance and application value in the fields of synthesis of secondary metabolites of orchid plants and medical application.

Provided herein are methods for predicting unobserved phenotypes and selecting genetic variant organisms for effective use in genetically improving non-human animal species. Also provided herein are systems for implementing such methods, as well as computer-readable storage media storing instructions for performing such methods.

The invention discloses application of an NtFATB gene in regulation and control of tobacco glandular hair development, and belongs to the technical field of plant genetic engineering. The NtFATB gene silent tobacco plant is cultivated by constructing an RNAi vector of the NtFATB gene, the tobacco leaf glandular hair density of the silent plant is observed, and analysis shows that the glandular hair density in the tobacco leaf is remarkably reduced. Therefore, the NtFATB gene is proved to be a key gene for regulating and controlling the development of the tobacco glandular hair. The gene is used in the field of scientific research and can be used for exploring a glandular hair development molecular regulation mechanism, identifying glandular hair development related genes, breeding key tobacco varieties for regulating glandular hair development and the like. The functional identification of the tobacco NtFATB gene not only enriches the gene resources of tobacco for regulating and controlling glandular hair development and quality breeding, but also is beneficial to analyzing the diversity function of the tobacco NtFATB gene, and has important theoretical significance and application value.

The invention discloses a key gene NtOS for controlling ocimene biosynthesis in tobacco and application of the key gene NtOS. The nucleotide sequence of the key gene NtOS for controlling ocimene biosynthesis in tobacco comprises a sequence as shown in SEQ ID No. 1, and the coded amino acid sequence comprises a sequence as shown in SEQ ID No. 2. The NtOS gene is transformed into tobacco through amplification, enzyme digestion, expression vector construction and agrobacterium infection, the ocimene release amount of an obtained NtOS gene transformed tobacco plant is remarkably increased, it is proved that the NtOS gene is a key gene for controlling ocimene biosynthesis, the function identification of the tobacco NtOS gene enriches the gene resources of tobacco quality breeding, and the tobacco NtOS gene has a wide application prospect. The method has important theoretical significance and application value.

Systems and methods for device functionality identification are disclosed. For example, a connected device may be coupled to a secondary device. A user may request operation of the connected device, and a system may determine that the connected device is of a given device type. Based on the connected device being of the given device type, the system may cause another device having an environmental sensor to send sensor data indicating environmental changes sensed by the sensor. The connected device may be operated and the sensor may sense environmental changes caused by operation of the connected device. When the sensed environmental changes indicate the device type of the secondary device, a recommendation to change the device type of the connected device to the device type of the secondary device may be provided to a user device associated with the connected device.

A method comprising, requiring to use a machine learning, ML, feature based on using a ML Model appropriate for the ML feature; receiving an activation message in relation to at least one of an activation of one ML Model available and appropriate for the ML feature, the one ML Model associated with at least one Functionality, or an activation of one Functionality of at least one Functionality associated with one ML Model available and appropriate for the ML feature, wherein the activation message indicates that the one ML Model and / or the one Functionality is activated; based on the received activation message, starting to use the one ML Model; and reporting at least one of ML Model performance key performance indicators, KPIs, of the one ML Model, or Functionality performance KPIs of the one Functionality associated with the one ML Model.

This invention provides a method, apparatus, and device for identifying closely related web pages based on function and structure. The method includes: acquiring page information of two web pages to be identified; extracting functional features from the two web pages, inputting the functional feature vectors into a functional classification model to predict the functional labels of the two web pages; searching for a corresponding clustering model in a clustering model set based on the functional labels; inputting the structural feature vectors into the corresponding clustering model to predict the cluster to which each of the two web pages belongs, wherein the clusters and the functional labels constitute the functional and structural features of the web pages; and determining whether the two web pages are closely related pages based on their respective functions and structures. This invention obtains the function of a page through functional identification, and then identifies the structure through structural clustering based on the functional information, thereby effectively characterizing the implementation structure of web pages and improving the accuracy of identifying closely related web pages.

A system for producing a therapeutic oligomer includes a computing device configured to design a proposed therapeutic oligomer sequence, wherein designing further comprises generating a genomic library for an organism from a gene target, initiating a sequence identification function, identifying a genomic locus that the proposed therapeutic oligomer sequence is predicted to bond to as a function of an off-target sequence function, selecting the proposed therapeutic oligomer sequence as a function of the sequence identification function, the genomic locus, and a criterion element, and synthesize a therapeutic oligomer as a function of the proposed therapeutic oligomer sequence.