14 results about "Functional identification" patented technology

The application belongs to the technical field of genetic engineering, and particularly relates to application of NtNRAMP2 protein, a method for improving tobacco cadmium stress resistance and a method for obtaining tobacco plants with cadmium stress resistance. The application provides application of NtNRAMP2 protein in creating tobacco with cadmium stress resistance or breeding tobacco with cadmium stress resistance, and an amino acid sequence of the NtNRAMP2 protein is shown as SEQ ID NO:2. The NtNRAMP2 protein is synthesized by NtNRAMP2 gene, and it is proved by transgenic and functional identification that the NtNRAMP2 natural resistance macrophage protein synthesized by the NtNRAMP2 gene is closely related to tobacco cadmium absorption regulation.

The application discloses a tobacco salt-tolerant gene Nt-zfhd-6 and application thereof. The nucleotide sequence of the tobacco salt-tolerant gene Nt-zfhd-6 comprises a sequence as shown in SEQ ID No. 1. The amino acid sequence coded by the tobacco salt-tolerant gene Nt-zfhd-6 is shown in SEQ ID No. 2. Under a salt stress condition, the expression amount of the Nt-zfhd-6 gene in tobacco seedling leaves is increased by nearly 40 times. The gene is amplified, enzyme-cut, and constructed into an expression vector, and then is infected into tobacco by means of agrobacterium, and a tobacco with the Nt-zfhd-6 gene is obtained. Under a salt stress treatment condition, the root length of the tobacco is obviously increased compared with that of a wild type, and it is proved that the Nt-zfhd-6 gene is a salt-tolerant gene. The functional identification of the tobacco Nt-zfhd-6 gene enriches the gene resources of tobacco salt-tolerant breeding, and has important theoretical significance and application value.

The invention discloses a method for synthesizing carnosic acid in vitro and application of the carnosic acid in improvement of oxidation resistance, and belongs to the field of molecular biology. According to the application disclosed by the invention, SrCYP716A514 and SrCYP76AK7 genes positioned in endoplasmic reticulum are found, the SrCYP716A514 and SrCYP76AK7 genes are found to be capable of synthesizing the carnosic acid in vitro, and the content of the carnosic acid is obviously increased when the SrCYP716A514 and SrCYP76AK7 genes are overexpressed in rosemary, and the oxidation resistance of a rosemary extract can be enhanced. Through separation and functional identification analysis of the SrCYP716A514 gene and the SrCYP76AK7 gene, it is determined that the SrCYP716A514 gene and the SrCYP76AK7 gene can synthesize carnosic acid in vitro, the content of the carnosic acid is remarkably increased through overexpression in rosemary, and the oxidation resistance of a rosemary extract can be enhanced. According to the invention, synthesis of carnosic acid in plants can be accurately regulated and controlled, the carnosic acid content can be increased, the method can be used for cultivating transgenic plants, plants with high carnosic acid content can be obtained, and the method has a wide application prospect in the fields of food, medicines, cosmetics and the like.

The invention discloses a dendrobium MYB transcription factor gene DoMYB59 and application of the dendrobium MYB transcription factor gene DoMYB59. The invention relates to application of a transcription factor DoMYB59 or a coding gene DoMYB59 thereof in regulation and control of synthesis of rutin in plants. The amino acid sequence of the transcription factor DoMYB59 is shown as SEQ ID NO.2. The invention also relates to application of the transcription factor DoMYB59 in regulation and control of synthesis of rutin in plants. A coding gene MYB transcription factor DoMYB59 belonging to a dendrobium MYB transcription factor DoMYB59 is cloned from dendrobium officinale leaves, the MYB transcription factor DoMYB59 is used as a positive regulation factor to participate in regulation of a rutin synthesis process of dendrobium, and transient expression and functional identification prove that the rutin content of transgenic dendrobium is increased when the MYB transcription factor DoMYB59 is excessively expressed. Therefore, the MYB transcription factor DoMYB59 or the coding gene thereof has important theoretical guidance significance and application value in the fields of secondary metabolite synthesis and medical application of orchid plants.

The invention discloses a robot acupoint identification system and method based on multi-physical field active disturbance and application. The method aims at solving the problems that traditional acupuncture point positioning depends on subjective experience, the automation degree is low, and positioning is not accurate only based on static physical characteristics. The robot comprises a vision-guided multi-degree-of-freedom mechanical arm; and the bionic end effector is mounted at the tail end of the mechanical arm. The core innovation of the actuator is that the actuator is integrated with a thermal-electric cooperative TENG sensing array (TE-TENG Array) and an active pressing unit. The TENG array can be switched between a sensing mode and an excitation mode; the actuator is further integrated with a miniature heat flow disturbance unit and a high-precision temperature sensor. The acupoints are defined and positioned by identifying extreme points of functional responses, the leap from static positioning to dynamic functional identification is achieved, and the accuracy and objectivity of automatic acupoint positioning are greatly improved.

A system for producing a therapeutic oligomer includes a computing device configured to design a proposed therapeutic oligomer sequence, wherein designing further comprises generating a genomic library for an organism from a gene target, initiating a sequence identification function, identifying a genomic locus that the proposed therapeutic oligomer sequence is predicted to bond to as a function of an off-target sequence function, selecting the proposed therapeutic oligomer sequence as a function of the sequence identification function, the genomic locus, and a criterion element, and synthesize a therapeutic oligomer as a function of the proposed therapeutic oligomer sequence.

The invention discloses a caulis sinomenii methyltransferase and an application of the caulis sinomenii methyltransferase in synthesis of sinomenine. The amino acid sequence of the caulis sinomenii methyltransferase is as shown in SEQ ID NO: 2. According to the invention, a new methyltransferase SaOMT3 is identified from a medicinal plant caulis sinomenii for the first time, and it is found that the methyltransferase participates in biosynthesis of sinomenine. Functional identification on SaOMT3 can greatly promote the research on the biosynthesis path of sinomenine, provide sufficient raw material guarantee for the creation of new sinomenine drugs, and also provide reference for the research of other medicinal plants. Meanwhile, the caulis sinomenii methyltransferase SaOMT3 disclosed by the invention is good in substrate specificity, high in catalytic efficiency and very high in application value.

The application belongs to the technical field of genetic engineering, and particularly relates to application of a transcription factor MdWRKY71 or a MdWRKY71 gene in promoting anthocyanin accumulation in plants, wherein an amino acid sequence of the transcription factor MdWRKY71 is shown as SEQ ID NO:1, and a nucleotide sequence of the MdWRKY71 gene is shown as SEQ ID NO:2. The transcription factor MdWRKY71 is synthesized by the MdWRKY71 gene, and it is proved by transgenic and functional identification that the transcription factor MdWRKY71 synthesized by the MdWRKY71 gene as a new transcription factor for regulating anthocyanin accumulation in apples can positively regulate expression of key anthocyanin synthesis enzyme genes MdANS and MdDFR, and promote 5-amino levulinic acid (ALA)-induced anthocyanin accumulation in apples, and can play an important role in agricultural and forestry production and scientific research.

This invention relates to a method for antibody reprogramming and screening based on engineered cells and its applications. This method achieves efficient in vitro rearrangement and diversity generation of antibody genes in non-lymphocytes by constructing engineered human cells containing the RAG1 / 2 recombinase system and programmable recombination signal sequence (RSS) elements. Utilizing this invention, the V(D)J rearrangement mechanism of B cells can be simulated in engineered cells, allowing for the targeted introduction of insertions, deletions, and point mutations in the antibody complementarity-determining region (CDR), resulting in a structurally diverse antibody library. Combined with piggyBac transposase-mediated genome integration, mammalian cell surface display technology, and flow cytometry sorting, high-throughput antibody screening and functional identification are achieved. This invention is simple to operate, has high antibody gene rearrangement efficiency, and produces a rich diversity of antibody libraries, making it suitable for rapid optimization of antibody drugs and discovery of new antibodies.

The application provides a functional identification of a novel virulence factor of a cell and an application thereof. Based on the research on the novel virulence factor of the cell, the application further provides a polypeptide delivery system and an application thereof. The polypeptide delivery system comprises a protein complex and a signal peptide, the signal peptide comprises at least 20 amino acids at the N-terminus of a PagT1 protein or a homologous protein thereof, the signal peptide is used for guiding the polypeptide to be loaded into the protein complex, and guiding the polypeptide to be positioned to the nucleus of a eukaryotic cell. The research of the application provides an important basis for constructing a safe and efficient novel tumor treatment system, and provides an innovative solution for the treatment of cancer and other major diseases.

The application discloses a kind of for synthesizing β - terpene synthase CcTPS6 of eucalyptol and its encoding gene and its product β - the application of eucalyptol as anti-aging drug belongs to synthetic biology and natural medicine chemistry technical field. The application starts from Labiatae plant torch flower ( Colquhounia coccinea var. mollis ) Cloning and functional identification of a synthetic β - eucalyptol single-function terpene synthase CcTPS6 encoding gene, its nucleotide sequence is as shown in Seq ID No.2, after codon optimization and site-directed mutagenesis, connect with expression vector pCold-TF, construct into the recombinant plasmid capable of expressing in escherichia coli, again the recombinant plasmid is transformed into escherichia coli and is constructed into engineering cell, realizes the heterologous high-efficiency synthesis compound β - eucalyptol in escherichia coli. The gene engineering cell constructed by the application is safe and stable, and the production cycle is short, which shows its great value in application development. The CcTPS6 product provided by the application β - eucalyptol has anti-aging activity and can be applied in the preparation of anti-aging drugs.

The present application provides a thermostable reductive aminase, its preparation method and application. Specifically, the present application relates to a thermostable reductive aminase and its functional identification and in vitro directed evolution. The reductive aminase of the present application is a reductive aminase from bacteria (BaRedAm) and its mutants (including a reductive aminase mutant with significantly improved enantiomeric selectivity of product rasagiline). The present application also provides an in vitro reductive amination method, comprising the steps of: (i) in the presence of a thermostable reductive aminase BaRedAm, subjecting (S1) a ketone substrate or an aldehyde substrate to a reductive amination reaction with (S2) an amine substrate. The enzyme and mutant of the present application can be used for in vitro enzymatic synthesis of different products such as enantiomerically pure rasagiline.

This invention discloses a method for constructing and applying a Linux module function identification model, belonging to the field of computer system security management technology. By analyzing the call relationships between modules, modules that depend on other modules are identified, thus obtaining upper-level modules. For each upper-level module, the functional information of the definition modules of several frequently called external functions is integrated to construct its function value matrix. By training the mapping relationship between the function value matrix and its function value vector of the upper-level modules, the correspondence between the function of the module to be identified and the functions of the other modules it depends on is learned, thereby constructing a Linux module function identification model. This allows for the reverse analysis of modules from unknown sources to obtain information about their dependent modules, and then, based on the mapping relationship between the functions of the dependent modules and their actual functions, accurate and effective module function identification can be performed.