143 results about "Functional identification" patented technology

A method and article of manufacture for encoding file metadata into a file name used in a computer system is disclosed. Metadata is added to an original file name and extension created by a user. The metadata may be in the form of a left padded, monotonically increasing number which operates similar to a time stamp. The file extension is then duplicated following a delimiter to preserve a user's ability to search for the original file name and extension, while maintaining functional identification of the file type to the operating and / or file system.

The invention relates to an invitro recombinant expression technology and functional identification of chlamys farreri peptidoglycan recognition protein (Cf-PGRP) gene. Cf-PGRP is provided with an amino acid sequence in a SEQ ID NO.1. The Cf-PGRP is obtained by the invitro recombinant expression technology, and the recombinant protein has broad sterilizing effect, obvious killing effect on Gram positive bacteria and the certain antibacterial effect on Gram negative bacteria and potential application value in aspects of developing broad antibacterial medicines, immune reinforcing agent, feed additive, and the like.

The invention discloses a method and equipment for realizing USB endpoint multiplexing, belonging to the field of communication. The USB equipment of the invention supports at least two USB functional devices and the number of the USB functional devices supported by the USB equipment is larger than the number of the data endpoints of the USB equipment. The method comprises the following steps: receiving the request of switching the USB functional devices, wherein the request carries the functional identifications of the USB functional devices selected by users; according to each functional identification, searching each USB functional device corresponding to the functional identification; and switching the USB equipment to the searched USB functional device. The equipment comprises USB equipment and a host. By the multiplexing of a USB endpoint, the invention realizes the purpose of taking the USB equipment as multifunctional equipment, and thereby saving the cost and improving the use experience of the user.

The invention provides an IEC (international electrotechnical commission) 61850 based verification system and an IEC 61850 based verification method for a switch mechanical characteristic testing device. The system is communicated with a to-be-tested device through an IEC 61850 network, and outputs state quantity and analog quantity to test the switch mechanical characteristic testing device. The verification method includes electric current accuracy testing, mechanism case temperature testing, energy storage medium pressure testing, opening and closing time testing, time synchronization function testing, diagnostic analysis function testing and repeatability testing. The verification system and the verification method have the advantages that various states of switchgears are replaced by analog signals and switching signals, so that repeatability is high, testing process is concise, and various working conditions, fault conditions and the like of the switchgears can recur; the verification system and the verification method are suitable for testing, functional identification and verification of the switch mechanical characteristic testing device in the electric power industry.

The invention discloses a broadband microscopic imaging spectrum system and a wavelength conversion rapid focusing control method thereof. The system comprises a microscope, a multiple optical paths switching device, an optical path controller, a light splitting unit, an Intensified Charge Coupled Device (ICCD), a light splitting unit controller, an image acquisition unit, a driving motor, an objective table, an objective table support, a position sensor, a light source and light splitting unit support, a focusing controller, a 2*1 optical path coupler, a reflection common light source, a reflection laser light source, a transmission common light source, a transmission laser light source, a light source controller, an image registering and controlling unit, a power supply module, and a computer. According to wavelength change, the system can automatically adjust a height of the objective table, rapid compensation of focal length change caused by the wavelength change is realized, and an acquired single wave band image can be subjected to pixel level registering. According to the invention, an imaging spectrum range is wide, ultraviolet, visible light and infrared light are covered, a microscopic image and spectrum information of a sample are obtained simultaneously, and the detection sample can be subjected to structural and functional identification and analysis.

A cloud control the access control management system and the authentication method used with one or a plurality of C-Lock connection management, the system includes at least: a system program and provide the management interface for programming operations, which contain five functional modules: a user module, a permission system module, a device management module, a virtual computing module and an event processing module combination; a database for storing the management information and function of identification information. Certification Department of the present invention is the use of multi-functional identification of cloud control lock mechanism through the encryption of network traffic and Cloud control the access control management system connection; it is close and remote two ways to achieve the purpose of certification.

The invention relates to a board cutting method based on automatic parameter recognition. The board cutting method comprises the following steps of S1, manufacturing a cutting band, inputting relatedparameters of a cutter in advance to manufacture a cutting band table; S2, manufacturing a cutter table, setting an identification code to be used as a functional identification point of a cutting machine host, and calling corresponding cutter parameters of the cutting band through the identification code in executing a cutter path; S3, drilling positioning holes in a PCB; S4, fixing the PCB on the cutting machine through pins on the positioning holes; S5, mounting a cutter and performing cutter alignment; S6, executing automatic cutting board manufacturing; S7, manufacturing the first piece and checking the dimensional accuracy; and S8 performing batch production and regular spot check. By virtue of the board cutting method, constant manual parameter input by a working staff can be eliminated; and each item of the cutter diameters, functions and parameters are pre-stored in the cutting band the cutting machine, so that a staff can realize direct starting up conveniently after the datais input, and influence to the product quality caused by wrong parameter input can be avoided.

The invention belongs to the field of genetic engineering, relates to separation, determinate evolution in vitro and functional identification of a resistance gene capable of degrading herbicide glyphosate efficiently, and further relates to the resistance gene capable of degrading herbicide glyphosate in plants. The nucleotide sequence of the gene B3S1 is as shown in the figure SEQ ID NO: 1; the amino acid sequence of encoded protein of the resistance gene is as shown in the figure SEQ ID NO: 2. According to the invention, the glycine oxidase (thiO) from marine bacteria bacillus cereus (Bacillus cereus) is amplified, and two rounds of random mutagenesis and a round of DNA shuffling test are performed continuously on the glycine oxidase (thiO), and combined with an activity screening technology on the basis of T7 phage pyrolysis-horseradish peroxidase / dianisidine enzyme coupling, the final glyphosate oxidase mutant B3S1 is obtained. The function of the gene and the application approach of the gene in degrading the resistance of herbicide glyphosate are proved in the invention.

The invention belongs to the field of plant biotechnology, and particularly relates to functional identification and application of a soybean constitutive expression promoter SOY001P. The promoter SOY001P disclosed by the invention has very high expression activity in tissues and organs such as roots, stems, leaves and flowers of plants, and has an excellent application prospect in the field of plant transgene.

The invention belongs to the technical field of plant biotechnology, and particularly relates to functional identification and application of a rice constitutive expression promoter KTN632P. A KT632 gene disclosed by the invention is a rice NAC type transcription factor family gene, so that the promoter thereof has very strong expression in tissues and organs such as roots, stems, leaves and flowers of plants, has very strong expressing activity under various abiotic stress treatment conditions, and has better application prospect in plant trasgenetic field.

The invention provides a method for rapidly displaying a functional control help document of an application program. The method comprises the following steps of: 10, monitoring long-press operation of a user on a functional control through a device; 20, acquiring long-press operation information and generating a corresponding identification code; 30, acquiring the identification code, and generating a corresponding functional identification code; 40, acquiring the functional identification code, and generating a prompt window for displaying the help document on the device; and 50, monitoring the prompt window, and stopping displaying the help document and closing the prompt window when the user performs short-press operation on the prompt window and operates the area beyond the prompt window. The method has the advantages that the method is convenient to operate and can effectively provide operating instructions and help the operator.

The invention relates to cloning and functional identification of a gene GhBZR1 for preferential expression of cotton fibers. The gene GhBZR1 is 1410bp in overall length and is coded with a protein containing amino acid 313. If a large amount of mRNA (messenger Ribonucleic Acid) of the gene GhBZR1 is accumulated in cotton fiber cells during a stretching development stage, the gene GhBZR1 is considered as a fiber preferential expression gene. The protein GhBZR1 is positioned in a cytoplasm and a cell nucleus, and is accumulatively increased in the cell nucleus after being induced by BL (Butyrolactone). The protein GhBZR1 can interact with a protein GhBIN2 and can be phosphorylated by the protein GhBIN2, thereby promoting the interaction carried out between the protein GhBZR1 and a protein 14-3-3. Thus, if the over-expression of the GhBZR1 is carried out in a mutant bri1 of arabidopsis BR, the scrubby phenotype of the arabidopsis BR can be restored. If the expression of the gene GhBZR1 in the cotton is inhibited by utilizing an RNAi (Ribonucleic Acid interfere) technique, the development of ovules and fibers of a transgenic cotton plant is apparently affected. Thus, the result speculates that the cotton fiber development and the fiber quality character are regulated by the gene GhBZR1 possibly in a manner of influencing a BR signal.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to separation, clone, functional identification and application of a rose DNA fragment. The rose DNA fragment comprises a rose transcription factor gene RrMYB11, wherein the nucleotide sequence of the rose transcription factor gene RrMYB11is shown in a sequence list SEQ ID NO: 1, and the corresponding protein sequence is shown in SEQ ID NO: 2. The gene fragment can change the flower type of a plant, and can be transferred into the plant directly to change the flower type of the transgenic plant.

The invention discloses a camera functional test plate comprising a big plate (100), a small plate (200) and a plurality of metal card lines (300), wherein, the big plate (100) is provided at least one big plate connector (101) used for connecting a head plate, and at least one group of big plate electric conduction columns provided with functional identifications, wherein, each big plate electric conduction column (102) is respectively connected with a pin of the big plate connector (101) correspondingly; the small plate (200) is provided with at least one small plate connector (201), and at least one group of small plate electric conduction columns (102), wherein, each small plate electric conduction column (102) is respectively connected with the pin of the small plate connector (201) correspondingly; and the metal card lines (300) are connected with the small plate electric conduction columns (102) and the big plate electric conduction columns (101) in a one-to-one correspondence mode. The camera functional test plate provided by the invention can be used for testing the functions of various camera modules, and can save the development time and cost.

The present invention is one kind of BS2 protein separated and purified from Bacillus subtilis B111 fermenting liquid and capable of resisting cotton blight and verticillum wilt. The initial functional identification shows that the BS2 protein possesses certain inhibition on cotton blight and verticillum wilt and E.coli. Identification shows that the BS2 protein belongs to neutral proteinase of Bacillus subtilis, and the gene sequence of the BS2 protein has been cloned from Bacillus subtilis B111. The BS2 protein may be used in the development of biological preparation for preventing and controlling cotton blight and verticillum wilt, and the gene may be used in the research of blight and verticillum wilt resisting transgenic cotton.

The invention discloses application of dendrobium officinale embryonic development later-period rich protein DoLEA43 to promoting the formation of plant callus. An encoding gene DoLEA43 of the dendrobium officinale embryonic development later-period rich protein DoLEA43 is obtained from dendrobium officinale protocorm through cloning, the dendrobium officinale embryonic development later-period rich protein DoLEA43 as the position regulation factor participates in the formation of the plant callus, the over-expression of the dendrobium officinale embryonic development later-period rich proteinDoLEA43 is proved through transgenic and functional identification, and the callus inductivity of a transgenic plant is increased. Thus, the dendrobium officinale embryonic development later-period rich protein DoLEA43 or the encoding gene thereof have important theoretical significance and application value in the plant tissue regeneration process and especially play an important role in preserving plant germplasm and generating active substances through the callus.

The invention discloses an antisense expression method for self-compatibility novel germplasm of an S-RNase creation pear by utilizing pollen mediators and belongs to the molecular genetics field. According to the antisense expression method for the self-compatibility novel germplasm of the S-RNase creation pear by utilizing the pollen mediators, an antisense expression vector pGSA1285-S3 is built through a double-enzyme-digestion method, the antisense expression vector pGSA1285-S3 is transferred to a juicy pear with yellow pear pollen serving as a vector through a method of processing the pollen mediators through ultrasonic waves, three plants containing the antisense expression vector are screened out according to molecular identification of progeny strains, and the novel germplasm of the self-compatibility pear is obtained. The antisense expression method for the self-compatibility novel germplasm of the S-RNase creation pear by utilizing the pollen mediators has the advantages of overcoming a defect that the existing self-compatibility pear is rare in variety resource, providing a new way for cultivating and innovating varieties of the self-compatibility pear, meanwhile providing good testing materials for functional identification of S-RNase gene protein products and having good breeding practical significances and theoretical research values.

The invention discloses vacuole proton pyrophosphatase as well as an encoding gene and application thereof and relates to the technical field of molecular biology and biology. A wheat TAVP gene is cloned, the functional identification is carried out in arabidopsis thaliana, a drought-relief and antisalt gene of the wheat is explored, and a foundation can be set for obtaining a good novel wheat strain. The vacuole proton pyrophosphatase has great significance in knowing the resistance mechanism of a plant, and a theoretical direction can be provided to assemble molecules, improve the crop stress resistance and improve the crop yield in the future.

The invention discloses an identification method of photoperiod sensibility quantization of wheat. Mainly aiming at Huanghuai winter wheat areas, the identification method of the photoperiod sensibility quantization of the wheat includes: planting a wheat material to be identified and a comparison material in different periods, wherein the planting periods include an early planting period and a late planting period at an interval of 40 days, and the last planting period ends before the end of November; performing regular farming management to avoid soil drought and fertilizer deficiency; accurately recording heading periods of every material in the early and the late planting periods; calculating difference between the heading periods of every material in the early and the late planting periods, and normally the difference is 1-20 days. The larger the difference between the heading periods is, the stronger the photoperiod sensibility of the material is, the smaller the difference between the heading periods is, the weaker the photoperiod sensibility of the material is, and thus the photoperiod sensibility of the wheat can be quantitatively evaluated. In addition, aiming at the photoperiod sensibility, phenotypic functional identification is performed directly, the photoperiod sensibility can be quantitatively identified, and the identification method is simple, convenient, fast and efficient in technology.

The invention discloses a tobacco outward-rectifying potassium ion channel gene NtSKOR1 as well as a cloning method and application thereof. The nucleotide sequence of the tobacco outward-rectifying potassium ion channel gene NtSKOR1 is as shown in SEQ ID NO. 1, and the amino acid sequence of encoded protein is as shown in SEQ ID NO. 2. The cloning method comprises the steps: synthesis of cDNA oftobacco leaves, specifically, extracting total RNA of the tobacco leaves, and performing reverse transcription to obtain a first strand of the cDNA; and PCR amplification of the NtSKOR1 gene, specifically, using the cDNA of the tobacco leaves as a template, designing a primer according to the NtSKOR1 gene sequence, performing PCR amplification, recycling and purifying PCR amplification products, and performing sequencing. The application is characterized in that the tobacco outward-rectifying potassium ion channel gene NtSKOR1 is applied to transgenic tobacco plants with the tobacco potassiumcontent regulated. The cloned NtSKOR1 gene is subjected to functional identification through a CRISPR / CAS9 technology. The cloning and identification of the tobacco NtSKOR1 gene provides a novel genetarget for regulating the potassium content of tobacco.

The invention discloses a tobacco protein kinase gene NtCIPK25-1 as well as a cloning method and application thereof. The nucleotide sequence of the tobacco protein kinase gene NtCIPK25-1 is as shownin SEQ ID NO. 1, and the amino acid sequence of encoded protein is as shown in SEQ ID NO. 2. The cloning method comprises the steps: synthesis of cDNA of tobacco leaves, specifically, extracting totalRNA of the tobacco leaves, and performing reverse transcription to obtain a first strand of the cDNA; and PCR amplification of the NtCIPK25-1 gene, specifically, using the cDNA of the tobacco leavesas a template, designing a primer according to the NtCIPK25-1 gene sequence, performing PCR amplification, recycling and purifying PCR amplification products, and performing sequencing. The application is characterized in that the tobacco protein kinase gene NtCIPK25-1 is used for obtaining transgenic tobacco plants with the tobacco potassium content regulated. The cloned NtCIPK25-1 gene is subjected to functional identification through a CRISPR / CAS9 technology. The cloning and identification of the tobacco NtCIPK25-1 gene provides a novel gene target for regulating the potassium content of tobacco.

The invention provides application of stress resistance related protein bHLH85 in regulation of plant stress resistance, and belongs to the technical field of biology. The invention reports a salt resistance negative regulation related protein bHLH85 and an encoding gene thereof for the first time. Research finds that the expression quantity of the bHLH85 gene in sweet sorghum is remarkably reduced under the salt stress condition, the bHLH85 gene is introduced into arabidopsis thaliana, functional identification is conducted on the arabidopsis thaliana, it is indicated that overexpression of the bHLH85 leads to increase of the number and the length of plant root hairs, and therefore the salt resistance of plants is reduced, and it is indicated that the bHLH85 plays a negative regulation role in salt resistance of plants, and a basis is provided for cultivating resistant plants.

The invention discloses a method for identifying an anti-disease gene of an apple by combining apple callus tissue cell culture and genetic transformation, particularly provides a method for identifying a target gene as a disease resistance-related gene of the apple. An apple callus tissue is taken to serve as a transformation receptor, and the target gene is identified as the disease resistance-related gene of the apple. Proved by tests, apple callus tissue transformation is utilized for functional identification of the anti-disease gene of the apple, technical barriers, including long plant transformation period, difficulties in transformation and the like of the apple, in anti-disease gene function research of the apple are overcome, screening efficiency of the anti-disease gene of the apple is increased greatly, an efficient and simple method for screening and identifying the anti-disease gene of the apple is provided, and exploration of the anti-disease gene of the apple is enabled to be possible. The method has the advantages of efficiency, easiness in implementation and short consumed time, and establishes a base and a possibility for anti-disease gene screening and identification of the apple and in-depth research of a mechanism of action of the anti-disease gene of the apple.

The invention provides a method for silencing a TRV-based virus induced primula malacoides genus plant gene. The method comprises the steps that A, according to a primula malacoides genus plant targetgene, a primer is designed, and a DNA fragment of the target gene is amplified; B, the DNA fragment is connected to a pTRV2 vector, and a recombinant vector is obtained; and C, a pTRV1 and the recombinant vector transform agrobacterium correspondingly, and obtained transformants commonly infect plants. According to the method, it is firstly proved that TRV can be successfully applied to gene silencing and functional identification of primula forbesii, and a foundation is laid for gene functional identification of primula malacoides genus plants. The method is simple, the research period is short, a full-length gene or a transformation system is not needed, the efficiency for obtaining a target phenotype is high, and quick functional identification of a large amount of genes can be achieved. compared with an existing VIGS system, the lasting time of phenotype of the method is long, phenotype changes appear on different parts of a plant, and the method has important significance on genetic research of self-incompatibility of primula malacoides genus heterotype.

The invention discloses a kit for identifying the enzyme activity of wild-type and mutant-type proteasome of X-linked hypophosphatemic rickets PHEX gene. The kit includes a wild-type and a mutant secretory-type PHEX gene and host cells. The kit can perform rapid functional identification of PHEX gene mutation, provide a target for designing drugs for further treatment of the disease, and provide atheoretical basis for elucidating the pathogenic mechanism of the disease.

The invention discloses Plagiochasma appendiculatum flavone hexahydroxy o-methyltransferase as well as an encoding gene and application thereof. The amino acid sequence of the Plagiochasma appendiculatum flavone hexahydroxy o-methyltransferase is one of the following sequences: (1) an amino acid sequence represented by SEQ ID No.1; or (2) a protein with an amino acid sequence obtained by virtue of substitution and / or deletion and / or addition of the amino acid sequence represented by SEQ ID No.1 through one or several amino acid residues and has the same function. O-methyltransferase capable of efficiently catalyzing the methylation of flavone hexahydroxy is cloned from Plagiochasma appendiculatum for the first time, and an in vitro enzyme activity functional identification experiment proves that optimal substrates of PAf6OMT are baicalein and scutellarein. An experiment for testing the influence of the reaction time and the protein amount to a substrate conversion rate proves that baicalein and scutellarein can be completely catalyzed to respectively generate aoroxylia A and hispidulin in a certain period of reaction time by virtue of a proper protein amount.

The invention discloses a geometric world clock table, characterized in comprising time line, hour minute scale identification, time number identificationú¼shadow regionú¼country name, functional identification, calendar minute second frame of the Western and Eastern Hemisphere, and 12 or 24 time lines merges all countries in the world and all geographical elements into an organic whole, it can in time display sports results of various countries such as results of the Olympic Games and automatically arrange a gold medal list, etc. in order, and simultaneously accurately display the time, time differences, geographical positions, calendars, weeks, day and night, weather, and scheduled flight of all countries in the world and it is also a geographical position distribution diagram and a time zone distribution diagram of various countries and by adding and subtracting time zone or counting country names, it can rapidly and accurately determine the time difference and relative geographical position between countries, having large information quantity, wide knowledge quantity, and compact disc face, easy to understand and read, and integrating strong timing function, knowledge, interest, popularization of science and humanized service into an organic whole.

The present invention relates to the isolation and functional identification of a novel acid and heat resistant trehalose synthase enzyme cloned from Picrophilus torridus. More particularly, the present invention discloses the DNA sequence for the Picrophilus torridus trehalose synthase gene, PTTS, which when expressed in a heterologous host such as Escherichia coli, provides enzymatic activity that catalyzes the direct interconversion of maltose and trehalose through intramolecular transglycosylation. Additionally, the present invention teaches methods of use of PTTS for production of trehalose as well as for production of various useful compounds comprising trehalose.

The invention relates to a shuttle plasmid pSW2 based on phage as well as a preparation method and an application of the shuttle plasmid pSW2. The base sequence of the shuttle plasmid is shown in SEQ ID NO.1; and a derivative plasmid is a promoter screening carrier taking a green fluorescent protein as a reporter gene. The preparation method comprises the following steps of: knocking out non-essential genes for copy expression of the plasmid out of the phage, fusing a chloramphenicol gene, cloning and replicating an initiation site, cloning a conjugal transfer component, inserting a multiple cloning site into a phage gene region, thus obtaining a pSW2 expression vector; and meanwhile, inserting the green fluorescent protein into the multiple cloning site, screening promoter sequences with different strengths, and constructing and generating the promoter screening vector. The shuttle plasmid provided by the invention can be replicated and expressed in escherichia coli and different shewanella baltica, can serve as a gene anaplerosis vector to be applied to the functional identification of shewanella baltica and can also be used for high-efficiency expression of exogenous genes at a low temperature.

The invention discloses an optimized tissue culture method for Columbia type arabidopsis thaliana and an application. The tissue culture method comprises steps as follows: KNO3 in a culture medium is replaced with KOH and HNO3 on the basis of an original MS macroelement formula, the content of HNO3 is reduced half, and a 1 / 2 nitrogen element optimized culture medium is obtained, wherein the culture medium comprises ingredients as follows: 1.25mM KH2PO4, 10.3mM NH4NO3, 1.5mM MgSO4*7H2O, 3 Mm CaCl2*2H2O, 18.8mM KOH and 9.4mM HNO3. The method is mainly used for improving the growth vigor of tissue culture Arabidopsis thaliana at the seedling stage, the survival rate of plants transplanted in the later period is ensured, and robust plants are cultured. The method can provide technical support for plant gene functional identification and research and accelerates the research progress of the plant functional genomics.