9 results about "Functional identification" patented technology

The application belongs to the technical field of genetic engineering, and particularly relates to application of NtNRAMP2 protein, a method for improving tobacco cadmium stress resistance and a method for obtaining tobacco plants with cadmium stress resistance. The application provides application of NtNRAMP2 protein in creating tobacco with cadmium stress resistance or breeding tobacco with cadmium stress resistance, and an amino acid sequence of the NtNRAMP2 protein is shown as SEQ ID NO:2. The NtNRAMP2 protein is synthesized by NtNRAMP2 gene, and it is proved by transgenic and functional identification that the NtNRAMP2 natural resistance macrophage protein synthesized by the NtNRAMP2 gene is closely related to tobacco cadmium absorption regulation.

This invention belongs to the field of genetic engineering technology, and specifically discloses the soybean BSP family. Glyma.20G130000.1 Application of a gene in soybean resistance to soybean Phytophthora root rot. This invention clones a gene from soybean. Glyma.20G130000.1 The expression was efficiently achieved in soybean plants through transient transformation, resulting in overexpressed soybean plants. Functional identification results showed that, compared with control plants, the overexpression... Glyma.20G130000.1 Soybean plants containing this gene exhibit significantly enhanced resistance to Phytophthora soybeanii. Furthermore, this invention provides the application of biomaterials related to this gene, and methods for screening or breeding disease-resistant soybean plants based on this gene. Therefore, Glyma.20G130000.1 Genes not only provide an effective tool for revealing the basic resistance mechanism of soybean to Phytophthora root rot, but also provide valuable genetic resources for soybean disease resistance breeding.

A system for producing a therapeutic oligomer includes a computing device configured to design a proposed therapeutic oligomer sequence, wherein designing further comprises generating a genomic library for an organism from a gene target, initiating a sequence identification function, identifying a genomic locus that the proposed therapeutic oligomer sequence is predicted to bond to as a function of an off-target sequence function, selecting the proposed therapeutic oligomer sequence as a function of the sequence identification function, the genomic locus, and a criterion element, and synthesize a therapeutic oligomer as a function of the proposed therapeutic oligomer sequence.

This invention relates to a method for antibody reprogramming and screening based on engineered cells and its applications. This method achieves efficient in vitro rearrangement and diversity generation of antibody genes in non-lymphocytes by constructing engineered human cells containing the RAG1 / 2 recombinase system and programmable recombination signal sequence (RSS) elements. Utilizing this invention, the V(D)J rearrangement mechanism of B cells can be simulated in engineered cells, allowing for the targeted introduction of insertions, deletions, and point mutations in the antibody complementarity-determining region (CDR), resulting in a structurally diverse antibody library. Combined with piggyBac transposase-mediated genome integration, mammalian cell surface display technology, and flow cytometry sorting, high-throughput antibody screening and functional identification are achieved. This invention is simple to operate, has high antibody gene rearrangement efficiency, and produces a rich diversity of antibody libraries, making it suitable for rapid optimization of antibody drugs and discovery of new antibodies.

The application provides a functional identification of a novel virulence factor of a cell and an application thereof. Based on the research on the novel virulence factor of the cell, the application further provides a polypeptide delivery system and an application thereof. The polypeptide delivery system comprises a protein complex and a signal peptide, the signal peptide comprises at least 20 amino acids at the N-terminus of a PagT1 protein or a homologous protein thereof, the signal peptide is used for guiding the polypeptide to be loaded into the protein complex, and guiding the polypeptide to be positioned to the nucleus of a eukaryotic cell. The research of the application provides an important basis for constructing a safe and efficient novel tumor treatment system, and provides an innovative solution for the treatment of cancer and other major diseases.

The application discloses a kind of for synthesizing β - terpene synthase CcTPS6 of eucalyptol and its encoding gene and its product β - the application of eucalyptol as anti-aging drug belongs to synthetic biology and natural medicine chemistry technical field. The application starts from Labiatae plant torch flower ( Colquhounia coccinea var. mollis ) Cloning and functional identification of a synthetic β - eucalyptol single-function terpene synthase CcTPS6 encoding gene, its nucleotide sequence is as shown in Seq ID No.2, after codon optimization and site-directed mutagenesis, connect with expression vector pCold-TF, construct into the recombinant plasmid capable of expressing in escherichia coli, again the recombinant plasmid is transformed into escherichia coli and is constructed into engineering cell, realizes the heterologous high-efficiency synthesis compound β - eucalyptol in escherichia coli. The gene engineering cell constructed by the application is safe and stable, and the production cycle is short, which shows its great value in application development. The CcTPS6 product provided by the application β - eucalyptol has anti-aging activity and can be applied in the preparation of anti-aging drugs.

The present application provides a thermostable reductive aminase, its preparation method and application. Specifically, the present application relates to a thermostable reductive aminase and its functional identification and in vitro directed evolution. The reductive aminase of the present application is a reductive aminase from bacteria (BaRedAm) and its mutants (including a reductive aminase mutant with significantly improved enantiomeric selectivity of product rasagiline). The present application also provides an in vitro reductive amination method, comprising the steps of: (i) in the presence of a thermostable reductive aminase BaRedAm, subjecting (S1) a ketone substrate or an aldehyde substrate to a reductive amination reaction with (S2) an amine substrate. The enzyme and mutant of the present application can be used for in vitro enzymatic synthesis of different products such as enantiomerically pure rasagiline.

This invention discloses a method for constructing and applying a Linux module function identification model, belonging to the field of computer system security management technology. By analyzing the call relationships between modules, modules that depend on other modules are identified, thus obtaining upper-level modules. For each upper-level module, the functional information of the definition modules of several frequently called external functions is integrated to construct its function value matrix. By training the mapping relationship between the function value matrix and its function value vector of the upper-level modules, the correspondence between the function of the module to be identified and the functions of the other modules it depends on is learned, thereby constructing a Linux module function identification model. This allows for the reverse analysis of modules from unknown sources to obtain information about their dependent modules, and then, based on the mapping relationship between the functions of the dependent modules and their actual functions, accurate and effective module function identification can be performed.