375 results about "Plant tissue" patented technology

The present invention discloses a multi-modal system for real-time plant hydration monitoring and irrigation management. The system comprises at least one acoustic sensor configured to emit controlled sound waves through plant tissues and detect corresponding vibrations and resonance frequencies using sensitive microphones or piezoelectric sensors to assess plant hydration. The system includes at least one bioelectrical sensor for monitoring bioelectrical signals. At least one nanotechnology-based sensor is configured to detect molecular changes in water content within plant cells through embedded or externally applied nanosensors. An aerial imaging device mounted on an unmanned aerial vehicle captures data related to canopy temperature, leaf color, and hydration indicators using infrared, multi-spectral, and thermal cameras. A central processing unit receives and analyzes data using predictive models, integrates the data via data fusion algorithm to generate a plant hydration profile, and controls water delivery through an irrigation management unit based on the hydration profile.

The invention discloses a method for predicting nitrogen oxide infection change of a plant based on an AI image in the technical field of plant research, and the method comprises the steps: building a dynamic mapping relation of morphological indexes-infection concentration and time; the image morphology of the plant under time lapse and / or other concentrations is directly predicted by inputting the current image of the plant, the plant tissue does not need to be damaged, the whole-stage dynamic monitoring of the same plant from the health state to the pollution stress can be realized through regular image acquisition, the interference of traditional chemical sampling on the plant growth is avoided, and the monitoring accuracy is improved. The method is particularly suitable for rare plant or long-term ecological research, the abstract RGB value and the nitrogen oxide concentration are converted into visual results such as a color gradient diagram and a dynamic feature point evolution curve, non-professionals can rapidly judge the pollution level through color depth and patch distribution, and the environment early warning response time is shortened.

The invention discloses a preparation method of artemisia apiacea extracellular vesicles, which comprises the following steps: step 1, pre-treating artemisia apiacea, carrying out primary centrifugal treatment, removing large plant tissues and cell debris, carrying out secondary centrifugal treatment, filtering by a needle filter, and collecting; step 2, transferring the filtered suspension to a sucrose density gradient solution, then carrying out third centrifugal treatment, collecting the solution with the concentration of 30% in the layer, and carrying out fourth centrifugal treatment to obtain artemisia apiacea extracellular vesicles; the invention further discloses an artemisia apiacea extracellular vesicle, a pharmaceutical composition for treating cerebral apoplexy, a medicine and application of the artemisia apiacea extracellular vesicle in preparation of the medicine for treating cerebral apoplexy. The naturally-sourced vesicle delivery system provided by the invention is expected to have higher safety and lower toxic and side effects, and the enrichment concentration of the therapeutic component at the focus part of cerebral apoplexy is improved, so that the bioavailability and the treatment efficiency of the medicine are remarkably improved.

The invention provides a plant exosome as well as an extraction method and application thereof in cosmetics, and belongs to the technical field of exosome extraction. The preparation method of the plant exosome comprises the following steps: mixing plant tissues with a solvent, carrying out high-pressure homogenization treatment, adding immobilized compound enzyme for enzymolysis, filtering, carrying out differential centrifugation on enzymatic hydrolysate, and carrying out size exclusion chromatography and ultrafiltration purification to obtain the plant exosome. Wherein exosome release and protection are considered, and the influence of agglomeration on the exosome extraction effect is avoided; high-pressure homogenization and immobilized compound enzyme enzymolysis are adopted, physical wall breaking is combined with chemical wall breaking, and the yield of the exosome is increased; the immobilized compound enzyme can avoid free enzyme residues, and the subsequent purification step is simplified; the plant exosome can be further purified through size exclusion chromatography and ultrafiltration. The exosome prepared by the method disclosed by the invention can retain the active ingredients of the exosome to the greatest extent, has excellent antioxidant, anti-inflammatory and whitening effects, and is suitable for the field of cosmetics.

A cotton variety FM 765AX is disclosed. Seeds, plants, plant cells, plant tissue, harvested products and cotton lint as well as to hybrid cotton plants and seeds obtained by repeatedly crossing plants of variety FM 765AX with other plants are also provided. Plants and varieties produced by the method of essential derivation from plants of FM 765AX and to plants of FM 765AX reproduced by vegetative methods, including but not limited to tissue culture of regenerable cells or tissue from FM 765AX are provided.

The invention provides a chitosan-iron nanoparticle leaf surface resistance and control agent as well as a preparation method and application thereof, and belongs to the technical field of reduction of heavy metal pollution of crops. The preparation method comprises the following steps: dissolving chitosan in an organic solvent to obtain a chitosan solution, mixing the chitosan solution with ferrous sulfate, adding a sodium tripolyphosphate solution for crosslinking and stirring to obtain a solution containing precipitates, centrifuging, and collecting the precipitates to obtain the chitosan / ferrous sulfate composite material. The TPP and amino groups of the chitosan form ionic crosslinking through triphosphate groups, and Fe < 2 + > is successfully loaded on the surfaces of the chitosan nanoparticles. By complexing chitosan and Fe < 2 + >, the crystallinity of the material is reduced, and the adsorption activity of the amorphous structure is enhanced. Pot experiments show that by spraying the chitosan-iron nanoparticle leaf surface resistance and control agent on leaf surfaces, heavy metals of crops can be remarkably reduced, the antioxidant enzyme activity of plant tissues is improved, active oxygen is effectively removed, oxidative damage is relieved, and therefore plant growth is promoted, and the toxic effect of heavy metal stress on the crops is relieved.

The invention relates to the technical field of life science, in particular to a full-automatic nucleic acid extraction system and process, and the system comprises a complete machine frame, a sample adding and waste liquid treatment mechanism, an oscillation magnetic suction heating mechanism, a control system and a screen interaction system. The sample adding and waste liquid treatment mechanism realizes three-dimensional accurate positioning through transverse and longitudinal movement guide rails and a vertical movement assembly, comprises a large piston device and a small piston device which are connected in parallel, and can flexibly switch different volumes of pipettes and share a connector; the oscillation magnetic suction heating mechanism integrates oscillation, heating and magnetic suction functions and is matched with multi-specification suction heads and kits to complete sample treatment. According to the extraction process, all the mechanisms are linked through a control system, and full-flow operation such as sample cracking, solid-liquid separation, magnetic bead purification and elution is automatically completed. The nucleic acid extraction device realizes automation and precision of nucleic acid extraction, improves the operation efficiency and the extraction effect, and is suitable for nucleic acid extraction of samples such as plant tissues, animal tissues, blood and plasmids.

The invention provides an extraction method and application of natural plant polyphenol, which mainly utilizes a polylactic acid-polyethylene glycol copolymer to prepare an extraction solution for extracting and embedding polyphenol in natural plant tissues. The preparation method comprises the following steps: (1) preparing a PLA-PEG extraction solution; (2) extracting the natural plant polyphenol to obtain the natural plant polyphenol extract; and (3) carrying out post-treatment on the natural plant polyphenol extract. The invention provides a new path for high-valued utilization of agricultural and sideline products and industrial upgrading of natural polyphenol. The stability of the polyphenol is remarkably improved and the shelf life is prolonged through copolymer embedding, long-acting bacteriostasis is achieved, and the hydrogel has good biocompatibility and wide market prospects.

The invention relates to the technical field of plant tissue extraction, in particular to an efficient extraction method and application of ginseng callus-derived exosomes, the extraction method comprises the following steps: S1, mixing ginseng callus with a PBS buffer solution, homogenizing and filtering to obtain a filtrate; s2, adding a protease inhibitor and a deep eutectic solvent into the filtrate, uniformly mixing, and standing to obtain a mixed solution; s3, centrifuging and filtering the mixed solution, and reserving supernate; s4, carrying out ultracentrifugation on the supernatant, collecting the precipitate, and resuspending with PBS to obtain a resuspension; and S5, further filtering the resuspension to obtain a filtrate, and storing at low temperature. The ginseng callus exosome extracted by the extraction method disclosed by the invention can be applied to preparation of drugs or cosmetics with anti-oxidation, anti-inflammatory and anti-aging effects, and the technical problems of low extraction efficiency, insufficient purity, complex extraction operation and poor stability of the extracted exosome in the existing exosome extraction technology can be effectively solved.

The invention provides a construction method of a plant tissue space transcriptome sequencing library, which comprises the following steps: fixing and embedding plant tissues to obtain embedded blocks; performing autofluorescence detection and tissue permeabilization treatment on part of the embedded blocks, and determining an autofluorescence threshold value and target tissue permeabilization time; slicing, pasting and fixing the remaining embedding blocks, and performing microscope fluorescence scanning detection and tissue permeabilization treatment on the tissue-containing chip according to an autofluorescence threshold value and target tissue permeabilization time to obtain a permeabilized tissue chip; and carrying out reverse transcription, tissue removal, cDNA release, recovery and amplification on the permeabilized tissue chip to obtain a cDNA amplification product. The plant tissue transcript constructed by the construction method is not easy to diffuse and high in capture rate, high-quality in-situ capture time-space group data can be obtained, the accuracy and the credibility are high, the sequencing accuracy is ensured, and the application value is high.

The invention discloses a fresh plant directed biotransformation method based on endogenous enzyme activation and a product and application thereof. The core of the preparation method is as follows: fresh plants (tuberous roots, stems or fruits) which are harvested in a ripe state and have metabolism in a dormant state (the respiration rate is lower than 5 mg CO / kg.h) are taken as raw materials, the raw materials are treated in an environment with the specific pressure being 0.02-0.20 MPa, the temperature being 70-121 DEG C and the humidity being 50-95%, a specific impregnating compound can be selectively combined, and the raw materials can be prepared into the plant culture medium. Under the conditions that exogenous microorganisms are not introduced and plant tissue structures are not damaged, the catalytic function of an endogenous metabolic enzyme system is safely and efficiently restarted and maximally promoted, and components of a plant body are driven to realize directional biotransformation. According to the process, conversion of primary metabolites and / or secondary metabolites is remarkably promoted, the content conversion of one or more beneficial components in the primary metabolites and / or the secondary metabolites is improved, and meanwhile, the biological and pharmaceutical structures and inherent metabonomics characteristics of the plants are completely reserved. The biotransformation product can be used as a high-quality raw material to be applied to medicines, common foods, health foods, special diet foods, daily chemical products and disinfection products, and can be further prepared into traditional Chinese medicine decoction pieces, solid preparations, semi-solid preparations and liquid preparations by adding or not adding pharmaceutically acceptable auxiliary materials.

A cotton variety FM 823AXTP is disclosed. Seeds, plants, plant cells, plant tissue, harvested products and cotton lint as well as to hybrid cotton plants and seeds obtained by repeatedly crossing plants of variety FM 823AXTP with other plants are also provided. Plants and varieties produced by the method of essential derivation from plants of FM 823AXTP and to plants of FM 823AXTP reproduced by vegetative methods, including but not limited to tissue culture of regenerable cells or tissue from FM 823AXTP are provided.

The invention discloses a radix gentianae callus cell culture and application method, and relates to the technical field of plant biology.The method comprises the following steps that in the laser irradiation process, a real-time spectrum analyzer is used for monitoring radix gentianae tissue, and absorption characteristic information of the plant tissue to light with different wavelengths is obtained. By combining the real-time spectral analysis and the machine learning model, the scheme can accurately monitor the interaction between the laser wavelength and the photosensitive component in the plant tissue, and by intelligently evaluating and dynamically adjusting the laser wavelength, the excessive absorption of laser energy is avoided, and the photochemical reaction and cell damage are reduced. The method not only reduces the potential damage of laser irradiation to plant cells, but also optimizes the synthesis process of medicinal components, and ensures the high efficiency, repeatability and reliability of callus culture. According to the scheme, powerful support is provided for improving the accuracy of experimental results and commercialized application of the gentiana scabra bunge.

The invention discloses a method for carrying out single-objective optimization on plant tissue culture based on artificial intelligence, and relates to the field of plant tissue culture, and the method comprises the following steps: building a plant tissue culture environment, and collecting and preprocessing environmental parameters; metabonomics data of plant tissues are measured through mass spectrum and liquid chromatography analysis, and a single-target optimization culture target is set in combination with environmental parameters; based on the environmental parameters and the metabonomics data, constructing a metabonomics analysis model through a random forest algorithm, and extracting a preliminary optimization path; according to the preliminary optimization path, using a sparse optimization algorithm to minimize plant culture resources, and obtaining a minimum resource scheme; plant tissue culture is carried out by using a minimum resource scheme, environmental parameters and metabonomics data are continuously collected, and metabonomics analysis model parameters are updated. By comprehensively utilizing artificial intelligence, metabonomics and an optimization algorithm, intelligence and precision of plant tissue culture and efficient utilization of resources are improved.

The invention belongs to the technical field of plant tissue culture, and particularly relates to an induction method of adventitious roots of cinnamomum camphora. According to the method disclosed by the invention, the adventitious root induction rate of the adventitious root of the cinnamomum camphora is remarkably improved by establishing a sterile system of the cinnamomum camphora, culturing cluster buds and sterile seedlings, promoting rooting of tissue culture seedlings, inhibiting browning, enabling the adventitious buds to extend and inducing the adventitious root through three ways, so that the problems of shortage of borneol resources and high market demand are solved; the method for inducing the adventitious roots of the cinnamomum camphora can be used for industrial production, and sufficient raw materials are provided for extraction of borneol; according to the method, large-scale culture of the adventitious roots of the cinnamomum camphora is achieved, a large number of pharmaceutical raw materials can be obtained at low cost in a short time, and an integrated production system can be formed with the pharmaceutical industry. The technology has important practical significance for promoting the development of the cinnamomum camphora industry, expanding the application of the pharmaceutical field, constructing a rare and endangered traditional Chinese medicinal material breeding technology system and realizing sustainable development and utilization.

The invention relates to a method for extracting genome DNA of plants rich in secondary metabolites and a buffer solution, and belongs to the technical field of molecular biology and botany. The method solves the technical problems of low DNA extraction efficiency, poor purity, easy degradation and the like when a traditional DNA extraction method is used for treating plant tissues rich in secondary metabolites such as alkaloid, polyphenol and the like. Comprising the following steps: adding a complexing agent such as polyethylene glycol or polyvinylpyrrolidone when grinding plant tissues in a liquid nitrogen environment; splitting by using a cell wall splitting buffer solution containing dithiothreitol and a nonionic surfactant; carrying out DNA release and extraction at 60-70 DEG C by adopting a CTAB (Cetyltrimethyl Ammonium Bromide) extraction buffer solution containing polyethylene glycol and papain; and then purifying and precipitating to obtain high-purity genome DNA (Deoxyribose Nucleic Acid). The method can effectively remove alkaloid, protein and other impurities, significantly improves the DNA yield and purity, and is suitable for genome sequencing, genetic resource protection, medicinal plant molecular identification and the like of plants with high secondary metabolites such as Stephania kwangsiensis and the like.

The present invention relates to a recombinant construct comprising: a first expression cassette comprising a fluorescence enhancing factor; wherein the amino acid sequence of the fluorescence enhancement factor is as shown in SEQ ID NO: 2, or an amino acid sequence which at least has 60%, 65%, 70%, 75%, 80%, 85% or 90% identity with the SEQ ID NO: 2 and is functionally equivalent to the protein as shown in the SEQ ID NO: 2; the first expression cassette is configured to increase the self-luminous intensity of a plant, plant tissue, or cell. According to the application, the LumEnhancer gene is further introduced on the basis of an FBP biological self-luminous system, so that the physiological activity of transgenic plant cells is effectively improved, and the energy metabolism level of the plant is further enhanced, thereby providing a more sufficient energy basis for a bioluminescence reaction and effectively enhancing the luminous intensity of the whole plant.

The utility model discloses an airlift type muskmelon outer vesicle culture and separation device which comprises a culture tank, the culture tank is a glass tank body with a big-end-up caliber, the top of the large caliber of the culture tank is connected with an exhaust valve through threads, and one side of the small caliber of the culture tank is fixedly connected with an air filter. One side of the air filter is fixedly connected with an air compressor, the culture tank is in threaded connection with a regulating valve, the output end of the regulating valve is connected with a pressure pump through a connecting pipe, the output end of the pressure pump is connected with an ultrafilter through a connecting pipe, and the bottom of the ultrafilter is provided with a discharge port; the outer surface of the side wall of the culture tank is in threaded connection with a temperature controller and a cooling water inlet, and the outer wall of the other side of the culture tank is in threaded connection with a cooling water outlet. The device is convenient to operate, fresh plant tissues do not need to be crushed traditionally, and the outer vesicles of the cucurbita moschata are cultured and enriched through airlift illumination suspension, so that the time and the labor cost are saved.

To provide a new method for efficiently purifying an exogenous protein expressed in a plant.SOLUTION: A method for purifying a foreign protein expressed in a plant, comprising the step of adding an acid to an extract of a tissue of a plant in which a foreign protein is expressed to degrade contaminating proteins derived from the plant.SELECTED DRAWING: None

The present invention discloses a plant tissue section redyeing method, which relates to the field of biotechnology. The method comprises the following steps: (1) preparing plant tissue sections; (2) observing the plant tissue sections for lignin autofluorescence; (3) subjecting the plant tissue sections observed in step (2) to fluorescent staining for cellulose; and (4) staining the plant tissue sections observed in step (3) with a brightfield dye. The present invention rationally arranges the dyes for use based on whether they are washable. By staining the same sample multiple times, the method is simple and quick, while avoiding errors caused by sample differences.

The present invention relates to a method for plant genome modification of at least one plant cell being in the developmental stage of a plant immature inflorescence meristem (IIM) cell, wherein the modification of the specific cell type is achieved by providing a genome modification or editing system, optionally together with at least one regeneration booster, preferably wherein the effector molecules are introduced by particle bombardment. To this end, new artificial and precisely controllable booster genes and proteins are provided. Further, the modified plant cells are regenerated in a direct or an indirect way. Finally, methods, tools, constructs and strategies are provided to effectively modify at least one genomic target site in a plant cell, to obtain the modified plant cell and to regenerate a plant tissue, organ, plant or seed from the modified plant cell.

This utility model relates to the field of life science technology, and in particular to a nucleic acid extraction device. The device includes a frame, a sample loading and waste liquid treatment mechanism, a oscillating magnetic heating mechanism, a control device, and a screen interaction system. The sample loading and waste liquid treatment mechanism achieves precise three-dimensional positioning through horizontal and vertical motion guides and vertical moving components. It includes parallel large and small pistons, allowing for flexible switching between different volume pipetting and sharing a common connector. The oscillating magnetic heating mechanism integrates oscillation, heating, and magnetic attraction functions, and works with various sizes of pipette tips and reagent kits to complete sample processing. The extraction process is automated through the control device, linking all mechanisms to automatically complete the entire process, including sample lysis, solid-liquid separation, magnetic bead purification, and elution. This application automates nucleic acid extraction, improving operational efficiency and extraction results, and is suitable for nucleic acid extraction from plant tissues, animal tissues, blood, plasmids, and other samples.

The present invention belongs to the field of microbial fermentation technology, and discloses a plant fermentation product with soothing and repairing effects, as well as its preparation method and application. The raw materials for preparing the plant fermentation product include: plant raw materials, solvents and fermentation strains; the plant raw materials include sea buckthorn fruit and licorice; the solvents include solvent A and solvent B, solvent A includes glycerol and / or ethoxydiglycol, and solvent B includes water; the fermentation strains include at least one of Lactobacillus plantarum, Lactobacillus casei and Lactobacillus fermentum. The present invention organically combines high-pressure homogenization and specific microbial fermentation, and high-pressure homogenization is supplemented with a specific solvent, so that plant tissue cells can be fully broken, components can be fully released and dissolved, and the utilization efficiency of plant raw materials can be improved. The plant composite liquid obtained by high-pressure homogenization is directly used as the subsequent fermentation matrix. Through the biotransformation ability of microorganisms, a plant fermentation product with a high content of active substances and significant soothing and repairing effects can be prepared.

Provided herein are methods for generating doubled haploid plants. The methods include obtaining a haploid plant tissue, contacting the haploid plant tissue with a safener compound, contacting the haploid plant tissue with a chromosome doubling agent, and regenerating a doubled haploid plant. The safener compound (e.g., metcamifen) and the chromosome doubling agent (e.g., colchicine) are applied sequentially or simultaneously. Additionally, the methods include further contacting the haploid plant tissue with a vigor response compound (e.g., thiamethoxam) in which the vigor response compound and chromosome doubling agent are applied sequentially or simultaneously.

The application provides cassava MeFER4 Gene and application thereof in improving sensitivity of plants to stress. By constructing overexpression vector containing MeFER4 Gene and transforming into host plant tissues such as Arabidopsis and cassava through infection method, plants sensitive to drought, abscisic acid, oxidation, salt and osmotic stress and other stress are obtained. Research shows that, MeFER4 It is a negative regulatory factor of plant stress response and plays a negative regulatory role in plant drought resistance and the like, and the transgenic plant is more sensitive to stress. The application is beneficial to elucidating the role of MeFER4 Gene in stress response from the molecular mechanism, and has important significance for directional genetic improvement of plant drought resistance and plant variety selection.

The present disclosure is directed to a split ribozyme biosensor system. Additionally, a genetically modified plant, plant tissue, or plant cell comprising the split ribozyme biosensor system is described. Methods for examining in vivo RNA expression in plants, plant cells or plant tissues are disclosed. Lastly, disclosed herein is a kit comprising the split ribozyme biosensor system.

This invention belongs to the field of biodetection technology, specifically relating to a primer set, kit, and application for detecting *Alternaria solani* brown spot disease. The base sequences of the primer set are shown in SEQ ID NO.1~SEQ ID NO.5. Based on the primer set, this invention utilizes loop-mediated isothermal amplification technology for the rapid detection of *Alternaria solani*, accurately detecting the fungus from the complex pathogenic environment in diseased plant tissues. The rapid detection system described in this invention exhibits high specificity and sensitivity, with a detection limit of 0.246 fg / μL for *Alternaria solani* DNA. Using the rapid detection system provided by this invention, *Alternaria solani* can be detected rapidly, conveniently, accurately, and sensitively from field samples within 45 minutes, which is simpler and more efficient than traditional symptom identification methods and ordinary molecular detection methods.

The invention discloses a gradient digestion, extraction and detection method for microplastics in plant tissues, and belongs to the technical field of plant pollutant detection. The core of the method is that a hydrogen peroxide-based primary digestion solution, a cellulase and pectinase compounded tissue digestion solution and a residual digestion solution composed of a Fenton reagent and sodium bromide are sequentially adopted to perform three-stage digestion on a plant sample under a mild acidic condition so as to thoroughly remove organic matters and completely retain micro-plastics. After digestion, micro-plastics are extracted through filtration, ultrasonic enrichment and concentration, and finally accurate qualitation and quantification are carried out through thermal cracking-gas chromatography / mass spectrometry. The invention synchronously protects the special digestion solution composition and the kit for realizing the method. According to the scheme, the contradiction between incomplete removal of organic matters and damage of a micro-plastic structure in a traditional digestion technology is effectively solved, the unification of efficient impurity removal (gt, 90%) and high recovery rate (greater than or equal to 85%) is realized, and the accuracy and reliability of detection are remarkably improved.

The invention discloses bacillus velezensis and application thereof in prevention and treatment of sweet potato black spot, and belongs to the field of microorganisms, 15 bacteria are successfully obtained through systematic separation of sweet potato rhizosphere soil and healthy plant tissues, screening is performed through a plate confrontation culture method, and the bacillus velezensis is obtained. The bacillus velezensis N-J-5 with a remarkable inhibiting effect on the sweet potato alternaria alternate is obtained. The antibacterial radius of the strain reaches 7.5 mm, and the antibacterial rate reaches up to 51.61%. And technologies such as morphological observation, physiological and biochemical characteristic determination, 16S rRNA gene sequence analysis and the like are comprehensively utilized to finally identify the bacillus velezensis as bacillus velezensis. The discovery of the strain provides a high-quality strain resource for research and development of a sweet potato black spot biological control preparation, and has important theoretical significance and practical value for promoting green and sustainable development of the sweet potato industry.

This invention discloses a kit and method for extracting cell nuclei from plant tissues, belonging to the field of biotechnology. The kit includes a nuclear lysis buffer and a nuclear washing buffer. The nuclear lysis buffer is prepared using nuclease-free water with sucrose, Tris-HCl (pH 7-8), NaCl, MgCl2, a surfactant, DTT, an RNase inhibitor, and spermidine. The nuclear washing buffer is prepared using nuclease-free water with sucrose, Tris-HCl (pH 7-8), NaCl, MgCl2, DTT, an RNase inhibitor, and spermidine. This kit is suitable for extracting cell nuclei from fresh yew stem tissue, yielding cell nuclei with intact nuclear membranes, stable gene expression, high purity, and few impurities, suitable for single-cell sequencing.