240 results about "Plant tissue" patented technology

The invention discloses a method for predicting nitrogen oxide infection change of a plant based on an AI image in the technical field of plant research, and the method comprises the steps: building a dynamic mapping relation of morphological indexes-infection concentration and time; the image morphology of the plant under time lapse and / or other concentrations is directly predicted by inputting the current image of the plant, the plant tissue does not need to be damaged, the whole-stage dynamic monitoring of the same plant from the health state to the pollution stress can be realized through regular image acquisition, the interference of traditional chemical sampling on the plant growth is avoided, and the monitoring accuracy is improved. The method is particularly suitable for rare plant or long-term ecological research, the abstract RGB value and the nitrogen oxide concentration are converted into visual results such as a color gradient diagram and a dynamic feature point evolution curve, non-professionals can rapidly judge the pollution level through color depth and patch distribution, and the environment early warning response time is shortened.

The invention discloses a preparation method of artemisia apiacea extracellular vesicles, which comprises the following steps: step 1, pre-treating artemisia apiacea, carrying out primary centrifugal treatment, removing large plant tissues and cell debris, carrying out secondary centrifugal treatment, filtering by a needle filter, and collecting; step 2, transferring the filtered suspension to a sucrose density gradient solution, then carrying out third centrifugal treatment, collecting the solution with the concentration of 30% in the layer, and carrying out fourth centrifugal treatment to obtain artemisia apiacea extracellular vesicles; the invention further discloses an artemisia apiacea extracellular vesicle, a pharmaceutical composition for treating cerebral apoplexy, a medicine and application of the artemisia apiacea extracellular vesicle in preparation of the medicine for treating cerebral apoplexy. The naturally-sourced vesicle delivery system provided by the invention is expected to have higher safety and lower toxic and side effects, and the enrichment concentration of the therapeutic component at the focus part of cerebral apoplexy is improved, so that the bioavailability and the treatment efficiency of the medicine are remarkably improved.

The invention relates to the technical field of life science, in particular to a full-automatic nucleic acid extraction system and process, and the system comprises a complete machine frame, a sample adding and waste liquid treatment mechanism, an oscillation magnetic suction heating mechanism, a control system and a screen interaction system. The sample adding and waste liquid treatment mechanism realizes three-dimensional accurate positioning through transverse and longitudinal movement guide rails and a vertical movement assembly, comprises a large piston device and a small piston device which are connected in parallel, and can flexibly switch different volumes of pipettes and share a connector; the oscillation magnetic suction heating mechanism integrates oscillation, heating and magnetic suction functions and is matched with multi-specification suction heads and kits to complete sample treatment. According to the extraction process, all the mechanisms are linked through a control system, and full-flow operation such as sample cracking, solid-liquid separation, magnetic bead purification and elution is automatically completed. The nucleic acid extraction device realizes automation and precision of nucleic acid extraction, improves the operation efficiency and the extraction effect, and is suitable for nucleic acid extraction of samples such as plant tissues, animal tissues, blood and plasmids.

The invention provides an extraction method and application of natural plant polyphenol, which mainly utilizes a polylactic acid-polyethylene glycol copolymer to prepare an extraction solution for extracting and embedding polyphenol in natural plant tissues. The preparation method comprises the following steps: (1) preparing a PLA-PEG extraction solution; (2) extracting the natural plant polyphenol to obtain the natural plant polyphenol extract; and (3) carrying out post-treatment on the natural plant polyphenol extract. The invention provides a new path for high-valued utilization of agricultural and sideline products and industrial upgrading of natural polyphenol. The stability of the polyphenol is remarkably improved and the shelf life is prolonged through copolymer embedding, long-acting bacteriostasis is achieved, and the hydrogel has good biocompatibility and wide market prospects.

The invention provides a construction method of a plant tissue space transcriptome sequencing library, which comprises the following steps: fixing and embedding plant tissues to obtain embedded blocks; performing autofluorescence detection and tissue permeabilization treatment on part of the embedded blocks, and determining an autofluorescence threshold value and target tissue permeabilization time; slicing, pasting and fixing the remaining embedding blocks, and performing microscope fluorescence scanning detection and tissue permeabilization treatment on the tissue-containing chip according to an autofluorescence threshold value and target tissue permeabilization time to obtain a permeabilized tissue chip; and carrying out reverse transcription, tissue removal, cDNA release, recovery and amplification on the permeabilized tissue chip to obtain a cDNA amplification product. The plant tissue transcript constructed by the construction method is not easy to diffuse and high in capture rate, high-quality in-situ capture time-space group data can be obtained, the accuracy and the credibility are high, the sequencing accuracy is ensured, and the application value is high.

The invention discloses a fresh plant directed biotransformation method based on endogenous enzyme activation and a product and application thereof. The core of the preparation method is as follows: fresh plants (tuberous roots, stems or fruits) which are harvested in a ripe state and have metabolism in a dormant state (the respiration rate is lower than 5 mg CO / kg.h) are taken as raw materials, the raw materials are treated in an environment with the specific pressure being 0.02-0.20 MPa, the temperature being 70-121 DEG C and the humidity being 50-95%, a specific impregnating compound can be selectively combined, and the raw materials can be prepared into the plant culture medium. Under the conditions that exogenous microorganisms are not introduced and plant tissue structures are not damaged, the catalytic function of an endogenous metabolic enzyme system is safely and efficiently restarted and maximally promoted, and components of a plant body are driven to realize directional biotransformation. According to the process, conversion of primary metabolites and / or secondary metabolites is remarkably promoted, the content conversion of one or more beneficial components in the primary metabolites and / or the secondary metabolites is improved, and meanwhile, the biological and pharmaceutical structures and inherent metabonomics characteristics of the plants are completely reserved. The biotransformation product can be used as a high-quality raw material to be applied to medicines, common foods, health foods, special diet foods, daily chemical products and disinfection products, and can be further prepared into traditional Chinese medicine decoction pieces, solid preparations, semi-solid preparations and liquid preparations by adding or not adding pharmaceutically acceptable auxiliary materials.

The invention relates to a method for extracting genome DNA of plants rich in secondary metabolites and a buffer solution, and belongs to the technical field of molecular biology and botany. The method solves the technical problems of low DNA extraction efficiency, poor purity, easy degradation and the like when a traditional DNA extraction method is used for treating plant tissues rich in secondary metabolites such as alkaloid, polyphenol and the like. Comprising the following steps: adding a complexing agent such as polyethylene glycol or polyvinylpyrrolidone when grinding plant tissues in a liquid nitrogen environment; splitting by using a cell wall splitting buffer solution containing dithiothreitol and a nonionic surfactant; carrying out DNA release and extraction at 60-70 DEG C by adopting a CTAB (Cetyltrimethyl Ammonium Bromide) extraction buffer solution containing polyethylene glycol and papain; and then purifying and precipitating to obtain high-purity genome DNA (Deoxyribose Nucleic Acid). The method can effectively remove alkaloid, protein and other impurities, significantly improves the DNA yield and purity, and is suitable for genome sequencing, genetic resource protection, medicinal plant molecular identification and the like of plants with high secondary metabolites such as Stephania kwangsiensis and the like.

The present invention relates to a recombinant construct comprising: a first expression cassette comprising a fluorescence enhancing factor; wherein the amino acid sequence of the fluorescence enhancement factor is as shown in SEQ ID NO: 2, or an amino acid sequence which at least has 60%, 65%, 70%, 75%, 80%, 85% or 90% identity with the SEQ ID NO: 2 and is functionally equivalent to the protein as shown in the SEQ ID NO: 2; the first expression cassette is configured to increase the self-luminous intensity of a plant, plant tissue, or cell. According to the application, the LumEnhancer gene is further introduced on the basis of an FBP biological self-luminous system, so that the physiological activity of transgenic plant cells is effectively improved, and the energy metabolism level of the plant is further enhanced, thereby providing a more sufficient energy basis for a bioluminescence reaction and effectively enhancing the luminous intensity of the whole plant.

The utility model discloses an airlift type muskmelon outer vesicle culture and separation device which comprises a culture tank, the culture tank is a glass tank body with a big-end-up caliber, the top of the large caliber of the culture tank is connected with an exhaust valve through threads, and one side of the small caliber of the culture tank is fixedly connected with an air filter. One side of the air filter is fixedly connected with an air compressor, the culture tank is in threaded connection with a regulating valve, the output end of the regulating valve is connected with a pressure pump through a connecting pipe, the output end of the pressure pump is connected with an ultrafilter through a connecting pipe, and the bottom of the ultrafilter is provided with a discharge port; the outer surface of the side wall of the culture tank is in threaded connection with a temperature controller and a cooling water inlet, and the outer wall of the other side of the culture tank is in threaded connection with a cooling water outlet. The device is convenient to operate, fresh plant tissues do not need to be crushed traditionally, and the outer vesicles of the cucurbita moschata are cultured and enriched through airlift illumination suspension, so that the time and the labor cost are saved.

To provide a new method for efficiently purifying an exogenous protein expressed in a plant.SOLUTION: A method for purifying a foreign protein expressed in a plant, comprising the step of adding an acid to an extract of a tissue of a plant in which a foreign protein is expressed to degrade contaminating proteins derived from the plant.SELECTED DRAWING: None

This utility model relates to the field of life science technology, and in particular to a nucleic acid extraction device. The device includes a frame, a sample loading and waste liquid treatment mechanism, a oscillating magnetic heating mechanism, a control device, and a screen interaction system. The sample loading and waste liquid treatment mechanism achieves precise three-dimensional positioning through horizontal and vertical motion guides and vertical moving components. It includes parallel large and small pistons, allowing for flexible switching between different volume pipetting and sharing a common connector. The oscillating magnetic heating mechanism integrates oscillation, heating, and magnetic attraction functions, and works with various sizes of pipette tips and reagent kits to complete sample processing. The extraction process is automated through the control device, linking all mechanisms to automatically complete the entire process, including sample lysis, solid-liquid separation, magnetic bead purification, and elution. This application automates nucleic acid extraction, improving operational efficiency and extraction results, and is suitable for nucleic acid extraction from plant tissues, animal tissues, blood, plasmids, and other samples.

Provided herein are methods for generating doubled haploid plants. The methods include obtaining a haploid plant tissue, contacting the haploid plant tissue with a safener compound, contacting the haploid plant tissue with a chromosome doubling agent, and regenerating a doubled haploid plant. The safener compound (e.g., metcamifen) and the chromosome doubling agent (e.g., colchicine) are applied sequentially or simultaneously. Additionally, the methods include further contacting the haploid plant tissue with a vigor response compound (e.g., thiamethoxam) in which the vigor response compound and chromosome doubling agent are applied sequentially or simultaneously.

The present disclosure is directed to a split ribozyme biosensor system. Additionally, a genetically modified plant, plant tissue, or plant cell comprising the split ribozyme biosensor system is described. Methods for examining in vivo RNA expression in plants, plant cells or plant tissues are disclosed. Lastly, disclosed herein is a kit comprising the split ribozyme biosensor system.

This invention belongs to the field of biodetection technology, specifically relating to a primer set, kit, and application for detecting *Alternaria solani* brown spot disease. The base sequences of the primer set are shown in SEQ ID NO.1~SEQ ID NO.5. Based on the primer set, this invention utilizes loop-mediated isothermal amplification technology for the rapid detection of *Alternaria solani*, accurately detecting the fungus from the complex pathogenic environment in diseased plant tissues. The rapid detection system described in this invention exhibits high specificity and sensitivity, with a detection limit of 0.246 fg / μL for *Alternaria solani* DNA. Using the rapid detection system provided by this invention, *Alternaria solani* can be detected rapidly, conveniently, accurately, and sensitively from field samples within 45 minutes, which is simpler and more efficient than traditional symptom identification methods and ordinary molecular detection methods.

The invention discloses a gradient digestion, extraction and detection method for microplastics in plant tissues, and belongs to the technical field of plant pollutant detection. The core of the method is that a hydrogen peroxide-based primary digestion solution, a cellulase and pectinase compounded tissue digestion solution and a residual digestion solution composed of a Fenton reagent and sodium bromide are sequentially adopted to perform three-stage digestion on a plant sample under a mild acidic condition so as to thoroughly remove organic matters and completely retain micro-plastics. After digestion, micro-plastics are extracted through filtration, ultrasonic enrichment and concentration, and finally accurate qualitation and quantification are carried out through thermal cracking-gas chromatography / mass spectrometry. The invention synchronously protects the special digestion solution composition and the kit for realizing the method. According to the scheme, the contradiction between incomplete removal of organic matters and damage of a micro-plastic structure in a traditional digestion technology is effectively solved, the unification of efficient impurity removal (gt, 90%) and high recovery rate (greater than or equal to 85%) is realized, and the accuracy and reliability of detection are remarkably improved.

The invention discloses bacillus velezensis and application thereof in prevention and treatment of sweet potato black spot, and belongs to the field of microorganisms, 15 bacteria are successfully obtained through systematic separation of sweet potato rhizosphere soil and healthy plant tissues, screening is performed through a plate confrontation culture method, and the bacillus velezensis is obtained. The bacillus velezensis N-J-5 with a remarkable inhibiting effect on the sweet potato alternaria alternate is obtained. The antibacterial radius of the strain reaches 7.5 mm, and the antibacterial rate reaches up to 51.61%. And technologies such as morphological observation, physiological and biochemical characteristic determination, 16S rRNA gene sequence analysis and the like are comprehensively utilized to finally identify the bacillus velezensis as bacillus velezensis. The discovery of the strain provides a high-quality strain resource for research and development of a sweet potato black spot biological control preparation, and has important theoretical significance and practical value for promoting green and sustainable development of the sweet potato industry.

The invention relates to the technical field of fermentation of medicinal material production, and discloses a construction method of a pseudo-ginseng cell line, which comprises the following steps: obtaining a target organ of a pseudo-ginseng plant; inducing calluses through a solid culture medium by adopting a target organ; and culturing and evaluating the callus in an oscillation fermentation system to obtain the working cell strain and the adaptive liquid culture medium thereof. According to the technical scheme provided by the invention, cells from different tissue parts of the same or different plants can form cell strain lines with different yields and different product components under selective and optimized culture conditions; the cell strain line can generate different drug properties and different economic values. In the dimensions, compared with traditional planting type pseudo-ginseng, the pseudo-ginseng planting method has the advantages of richness of germplasm and diversity of product application. When the obtained working cell strain is applied to a liquid fermentation method of pseudo-ginseng cells, compared with a traditional pseudo-ginseng planting method, pseudo-ginseng biomass which is richer in medicine property and higher in quality is more efficiently produced.

The application discloses a functional gene PaSPL8 for regulating apricot fruit ripening, and the nucleotide sequence of the PaSPL8 gene is shown as SEQ ID NO. 1. The functional gene PaSPL8 for regulating apricot fruit ripening is used for regulating apricot or tomato fruit ripening, and the fruit ripening is delayed by increasing the expression amount of the PaSPL8 gene; or the functional gene PaSPL8 for regulating apricot fruit ripening is used for regulating the content of abscisic acid in plant tissues. The application verifies that the PaSPL8 gene regulates the ripening of apricot fruits by regulating the content of ABA in apricots, and therefore, the PaSPL8 gene can be used for apricot breeding.

The application provides a method for obtaining aseptic endocarp of rubber tree young fruit, and belongs to the technical field of plant tissue engineering. The method for obtaining aseptic endocarp of rubber tree young fruit through standard anatomical operation is realized without using ethanol and mercury chemical disinfectants, and the aseptic endocarp of rubber tree young fruit is obtained. The method eliminates the ethanol and mercury disinfection steps, completely avoids the use of toxic mercury chemical reagents, eliminates the potential harm to the operators and the environment, and meets the green biological technology standard. In addition, the method does not need to prepare, manage and dispose of dangerous chemical reagents, simplifies the process, reduces the cost of consumables, and can obtain aseptic endocarp only through standard anatomical operation. The method utilizes the natural barrier effect of the young fruit peel to realize the aseptic acquisition of the internal seeds, and the pollution rate can be stably controlled below 2%, and the aseptic acquisition rate is above 98%.

The application discloses a kind of biological reaction devices of plant tissue, comprising, culture device, for the culture of plant tissue, the outer wall of the culture device is provided with detection device, for detecting the parameter and / or sampling of culture solution in the culture device;Heat transfer structure, for the temperature control of the culture device, the heat transfer structure has notch, the detection device is arranged on the outer wall of the culture device in the notch.The plant tissue is cultured by the culture device in the application, the temperature in the device is kept stable by heat transfer structure, prevent the influence caused by external temperature to the environment in the device.And detection device is arranged on the outer wall of the culture device in the notch of heat transfer structure, can avoid the damage or heat loss of heat transfer structure caused by detection device passing through heat transfer structure, ensure the integrity of heat transfer structure, make temperature keep stable, plant tissue better growth.

The invention relates to biotechnology and provides novel engineered protein for conferring enhanced growth. The invention also provides transgenic plants, seeds, cells and plant parts containing recombinant DNA molecules, as well as methods of using the same.

This invention relates to the field of agricultural breeding technology and discloses an integrated device for precise sampling and labeling of single wheat breeding materials. The device includes a feeding drive mechanism, a photoacoustic coaxial cutting and in-situ labeling module, a carrier-type collection system, a microfluidic system, and a central control system. The device utilizes high-frequency mechanical vibration to cut plant tissue, combined with cavitation effects to prevent sample contamination from the cutting edge. The cut sample falls into the carrier for sealed encapsulation and digital identification. The module pumps liquid photosensitive resin to the plant wound, and through ultrasonic-induced shear thinning and acoustic flow effects, promotes deep resin penetration. A coaxial ultraviolet beam is then output for in-situ curing, forming a peel-resistant, mechanically interlocked label patch. The main control system extracts the sample identifier, timestamp, and spectral characteristics of quantum dots within the resin, generating an encrypted hash value for persistent storage. This invention achieves high-purity, precise sampling, robust in-situ labeling, and tamper-proof multidimensional data traceability.

The invention discloses a strain sensor for monitoring plant growth and application thereof, and belongs to the technical field of hydrogel strain sensors. According to the strain sensor for monitoring plant growth, PGTA hydrogel is synthesized through a one-pot method, the PGTA hydrogel is solidified on plant stalks in situ, and then flexible carbon cloth is used for conducting wire leading-out to a data acquisition system; under the condition that plant tissues are not damaged, the fidelity and the signal-to-noise ratio of detection electric signals are improved, compared with existing documents, the durability of the hydrogel strain sensor is improved, an in-situ polymerization method is utilized for the first time to construct the hydrogel strain sensor to achieve in-situ sustainable monitoring of plant growth, and the application prospect is wide. A wireless transmission system is used to realize synchronous monitoring of plant moisture information and growth information, and an internal time relationship between the plant moisture information and growth strain is explored.

Provided is a method for generating a plant having improved growth and / or yield. The method comprises selectively increasing the size of one or more guard cells in a plant wherein the guard cell size is increased compared to guard cell size in an independent wild type control plant of the same species under the same environmental conditions. The ratio of guard cell size to leaf epidermal cell size in the plant is increased as compared to the ratio in the control plant. Also provided is a plant obtainable by the method; a gene construct; a transformed plant part or plant progeny; and a plant part, plant tissue, plant organ, plant cell, plant protoplast, embryo, callus culture, pollen grain or seed, derived or obtained from the plant.

The invention discloses an ingenol ABC transport protein ElABCG39 as well as a coding gene and application thereof. The protein is positioned in a cytoplasmic membrane, and expression of the protein in yeast proves that the protein has ingenol transmembrane efflux transport function, improves the ingenol tolerance of the yeast, and proves that the ElABCG39 has certain substrate specificity in the yeast. The ingenol ABC transporter ElABCG39 can be used for producing ingenol by fermentation of microorganisms or plant tissues.

The plant chemical type rapid collection device and collection method provided by the application, wherein the plant chemical type rapid collection device comprises a mounting seat, a pull rod and a sampling needle wrapped with stainless steel fibers, the pull rod is coaxially connected with the sampling needle and is arranged in the axial direction of the mounting seat, a sealing sleeve is arranged on the side end face of the mounting seat facing the sampling needle, and the sampling needle can be accommodated in the sealing sleeve; the stainless steel fibers are provided with a silica resin-PMSQ microsphere coating. The plant chemical type rapid collection device and collection method provided by the application adopt a new type of polymethylsilsesquioxane microsphere coating, so that the plant chemical type rapid collection device, especially the sampling needle, has the characteristics of high temperature resistance, good acid and alkali resistance and high extraction efficiency, and can fully absorb plant tissue components in a short time; the plant chemical type rapid collection device is designed and manufactured with a sealing sleeve, and can temporarily store plant samples for a long time.

The invention relates to plant source dry-state mitochondria for cell repair and aging resistance as well as a preparation method and application of the plant source dry-state mitochondria. The plant source dry-state mitochondria can effectively improve functions of mitochondria, reduce the damage degree of cells, inhibit cell aging and effectively repair skin wounds. According to the method, the mitochondria is extracted from healthy plant tissues, so that the production cost is reduced, and a harsh quarantine step is not needed. According to the present invention, the dry-state processing enables the mitochondria storage condition to be loose, the transportation and storage difficulty and the transportation and storage cost can be further reduced, the plant source mitochondria subjected to the dry-state processing has characteristics of flexible dosage form, wide auxiliary material application range, and meeting of the design requirements of various products, and the dry-state processing can further remove the nucleic acid in the extract and eliminate the inflammation of the product; in the research process, the inventor also finds that after the plant source mitochondria is subjected to dry-state processing, the mitochondria function can be more effectively improved, the cell damage degree is reduced, cell aging is inhibited, and the skin wound surface is effectively repaired.

Described herein are methods for producing a modified Rubus species plant or plant part. The method can include introducing a polynucleotide of interest (e.g., a portion of an editing system) and a gene that confers resistance to a selection agent into one or more meristem cells of a wounded tissue including meristem cells to provide a transformed tissue, culturing the transformed plant tissue in the presence of the selection agent in an amount at least 1.5 times that needed to kill 90% of Rubus spp. cells to provide a cultured transformed tissue, and regenerating a Rubus spp. plant or plant part from the cultured transformed tissue.

An object of the present invention is to provide a penetration promoter and an agricultural composition, which are capable of promoting penetration of an organic substance into a plant tissue. A penetration promoter for an organic substance for plants, containing at least one compound selected from an oxo fatty acid, or a derivative thereof or a salt thereof, and a hydroxy fatty acid, or a derivative thereof or a salt thereof. An agricultural composition containing: (a) at least one compound selected from an oxo fatty acid, or a derivative thereof or a salt thereof, and a hydroxy fatty acid, or a derivative thereof or a salt thereof; and (b) at least one organic substance for plants.

The utility model provides an aerosol generating product and an aerosol generating system. Wherein the aerosol-generating article comprises: an atomizing element comprising an aerosol-forming agent configured to generate an aerosol when heated; the active functional element is arranged at the downstream of the atomizing element; the active functional element comprises an active matrix; in use, when aerosol generated by the atomizing element flows downstream through the active functional element, the aerosol entrains one or more volatile components released by the active matrix and then is delivered downstream; the active substrate is a porous structure through which an air flow can pass. When the aerosol generating product is used, on one hand, the aerosol forming agent and the active functional components are in different elements, compared with a mode that the aerosol forming agent and the active functional components are completely loaded on plant tissues, the additive amount can be larger, and the smoke amount and the fragrance amount can be increased; and on the other hand, the active matrix is gradually released to form slow release, which is beneficial for maintaining the uniformity of active components with different suction.