490 results about "Plant tissue" patented technology

The present invention discloses a multi-modal system for real-time plant hydration monitoring and irrigation management. The system comprises at least one acoustic sensor configured to emit controlled sound waves through plant tissues and detect corresponding vibrations and resonance frequencies using sensitive microphones or piezoelectric sensors to assess plant hydration. The system includes at least one bioelectrical sensor for monitoring bioelectrical signals. At least one nanotechnology-based sensor is configured to detect molecular changes in water content within plant cells through embedded or externally applied nanosensors. An aerial imaging device mounted on an unmanned aerial vehicle captures data related to canopy temperature, leaf color, and hydration indicators using infrared, multi-spectral, and thermal cameras. A central processing unit receives and analyzes data using predictive models, integrates the data via data fusion algorithm to generate a plant hydration profile, and controls water delivery through an irrigation management unit based on the hydration profile.

The invention discloses a method for predicting nitrogen oxide infection change of a plant based on an AI image in the technical field of plant research, and the method comprises the steps: building a dynamic mapping relation of morphological indexes-infection concentration and time; the image morphology of the plant under time lapse and / or other concentrations is directly predicted by inputting the current image of the plant, the plant tissue does not need to be damaged, the whole-stage dynamic monitoring of the same plant from the health state to the pollution stress can be realized through regular image acquisition, the interference of traditional chemical sampling on the plant growth is avoided, and the monitoring accuracy is improved. The method is particularly suitable for rare plant or long-term ecological research, the abstract RGB value and the nitrogen oxide concentration are converted into visual results such as a color gradient diagram and a dynamic feature point evolution curve, non-professionals can rapidly judge the pollution level through color depth and patch distribution, and the environment early warning response time is shortened.

The present disclosure provides genetically modified plants, plant cells, or plant tissues wherein the plants, plant cells, or plant tissues comprise an exogenous nucleic acid comprising a nucleotide sequence encoding a 30S ribosomal protein or a homolog thereof, wherein the 30S ribosomal protein or the homolog thereof is expressed in the plant, plant cell, or plant tissue. The current disclosure provides methods of improving drought tolerance and water loss in a plant, plant cell, or plant tissue, the methods comprising an exogenous nucleic acid sequence encoding a 30S ribosomal protein or a homolog thereof, wherein the 30S ribosomal protein or the homolog thereof is expressed in the plant, plant cell, or plant tissue. Additionally, the current disclosure is describes to genetically modified plants, plant cells, or plant tissues wherein the plants, plant cells, or plant tissues comprise an exogenous nucleic acid comprising a nucleotide sequence encoding a cation / H+ exchanger 20 (CHX20) protein or a homolog thereof, wherein the nucleotide sequence is operably linked to a heterologous promoter, and the CHX20 protein or homolog thereof is expressed in the plant, plant cell, or plant tissue. Finally, the current disclosure provides methods of improving drought tolerance and water loss in a plant, plant cell, or plant tissue, the methods comprising an exogenous nucleic acid sequence encoding a CHX20 or a homolog thereof, wherein the CHX20 or the homolog thereof is expressed in the plant, plant cell, or plant tissue.

The invention discloses a preparation method of artemisia apiacea extracellular vesicles, which comprises the following steps: step 1, pre-treating artemisia apiacea, carrying out primary centrifugal treatment, removing large plant tissues and cell debris, carrying out secondary centrifugal treatment, filtering by a needle filter, and collecting; step 2, transferring the filtered suspension to a sucrose density gradient solution, then carrying out third centrifugal treatment, collecting the solution with the concentration of 30% in the layer, and carrying out fourth centrifugal treatment to obtain artemisia apiacea extracellular vesicles; the invention further discloses an artemisia apiacea extracellular vesicle, a pharmaceutical composition for treating cerebral apoplexy, a medicine and application of the artemisia apiacea extracellular vesicle in preparation of the medicine for treating cerebral apoplexy. The naturally-sourced vesicle delivery system provided by the invention is expected to have higher safety and lower toxic and side effects, and the enrichment concentration of the therapeutic component at the focus part of cerebral apoplexy is improved, so that the bioavailability and the treatment efficiency of the medicine are remarkably improved.

The invention relates to the technical field of plants, in particular to a paraffin embedding method for reducing plant RNA degradation. In the plant paraffin embedding method provided by the invention, a stationary liquid for fixing plant tissues is prepared from absolute ethyl alcohol and acetic acid in a volume ratio of (2-5): (1-4); buffer I used for dehydration is absolute ethyl alcohol and n-butyl alcohol with the volume ratio of (1-3): (1-2); buffer II used for waxing is n-butyl alcohol and low-temperature paraffin wax with the volume ratio of (1-3): (1-2), and the melting point of the paraffin wax is 20-50 DEG C. According to the method, low-temperature paraffin with the melting point of 20-50 DEG C is adopted, and a reagent for paraffin embedding and a paraffin embedding process are adjusted, so that the plant section with relatively high RNA integrity and relatively high tissue structure integrity is obtained. The paraffin embedding method provided by the invention is suitable for different types of molecular biology experiments of space transcriptome, immunohistochemistry, immunofluorescence and the like.

The invention relates to a biochar material for repairing cadmium and tetracycline combined pollution in a water body as well as preparation and application of the biochar material, and belongs to the technical field of water body pollution repairing. Comprising the following steps: (1) cleaning, drying and grinding plant tissues of solanum nigrum plants after the cadmium-polluted soil is repaired by fungi and solanum nigrum, so as to obtain solanum nigrum plant tissue powder; and (2) pyrolyzing the black nightshade plant tissue powder at 400-600 DEG C for 1-3 hours, cooling to 20-30 DEG C, and grinding to obtain the biochar material for repairing cadmium and tetracycline combined pollution in the water body. According to the invention, the plant tissue of the nightshade plant after the cadmium-polluted soil is repaired by the combination of fungi and nightshade is used as the raw material of the biochar material for the first time, so that the resource utilization of the polluted soil repairing plant is realized. The problem of plant treatment after polluted soil remediation is solved, consumption of extra resources is avoided, and the sewage treatment cost is greatly reduced.

The invention provides a plant exosome as well as an extraction method and application thereof in cosmetics, and belongs to the technical field of exosome extraction. The preparation method of the plant exosome comprises the following steps: mixing plant tissues with a solvent, carrying out high-pressure homogenization treatment, adding immobilized compound enzyme for enzymolysis, filtering, carrying out differential centrifugation on enzymatic hydrolysate, and carrying out size exclusion chromatography and ultrafiltration purification to obtain the plant exosome. Wherein exosome release and protection are considered, and the influence of agglomeration on the exosome extraction effect is avoided; high-pressure homogenization and immobilized compound enzyme enzymolysis are adopted, physical wall breaking is combined with chemical wall breaking, and the yield of the exosome is increased; the immobilized compound enzyme can avoid free enzyme residues, and the subsequent purification step is simplified; the plant exosome can be further purified through size exclusion chromatography and ultrafiltration. The exosome prepared by the method disclosed by the invention can retain the active ingredients of the exosome to the greatest extent, has excellent antioxidant, anti-inflammatory and whitening effects, and is suitable for the field of cosmetics.

Provided herein are nanofibres including self-assembled cellular components derived from a homogenized plant tissue. Methods for preparing such nanofibres, as well as uses thereof in the treatment or prevention of diseases or disorders in a subject and / or as delivery vehicles are also described.

The invention discloses a light formula generation method and system based on dendrobium officinale polysaccharide synthesis, particularly relates to the technical field of plant factory light environment regulation and control, and is used for solving the technical bottlenecks of polarization state instability and insufficient light signal penetration uniformity caused by environment interference in existing polarization state light regulation and control. A polarization state distortion area is sensed in real time based on Stokes parameters, physical interference factors of a reflection path and medium scattering are extracted, polarization state compensation parameters are generated through cross-correlation modeling, a laser light source direction angle and a phase delay amount are cooperatively optimized, and re-calibration of a distortion matrix is triggered through colorimetric method penetration uniformity verification; through closed-loop logic of physical interference traceability and dynamic compensation, the polarization stability of optical signals and the penetrability of plant tissues are synchronously improved, and efficient cooperation of a dendrobium officinale light response mechanism and a polysaccharide synthesis path is guaranteed.

The invention relates to the technical field of plant genetic engineering, in particular to a ginkgo GbGRF2 gene and application of a GbGRF2-GbGIF2 fusion gene of the ginkgo GbGRF2 gene in plant tissue culture. According to the invention, the GbGRF2 gene coding region full length of the GRF gene family of gingko is successfully cloned from gingko, and the gene presents a specific expression mode in the regeneration process of gingko callus; the overexpression of the fusion gene of GbGRF2 and GbGRF2-GbGIF2 can significantly promote the regeneration efficiency of adventitious buds in the in-vitro culture process of plants; the gene provided by the invention has important theoretical significance and application potential in a plant tissue regeneration regulation network, and plays a key role in improving in-vitro regeneration efficiency of gymnosperm.

The invention provides a movable crop stress in-vivo recognition device and a stress type recognition method, and the device comprises a fixed support which is provided with a supporting rod extending in the transverse direction; the transverse displacement rod extends in the longitudinal direction, is arranged on the supporting rod in a sliding mode and is used for moving on the supporting rod in the transverse direction; the longitudinal displacement platform is arranged on the transverse displacement rod in a sliding mode and used for moving on the transverse displacement rod in the longitudinal direction; and the Raman spectrum acquisition assembly is arranged on the longitudinal displacement platform and is used for acquiring a Raman spectrum on the target plant. According to the movable crop stress in-vivo recognition device and the stress type recognition method provided by the invention, the advantages of no need of sample pretreatment and high specificity of a Raman spectrum technology are utilized, stress response marker molecules in plant tissues are detected, and related information of a plant physiological state is obtained; and in-vivo, lossless and rapid identification of crop stress is realized by matching with structures such as a fixed bracket, a transverse displacement rod and a longitudinal displacement platform.

The invention discloses a gradient internal reference-based absolute quantification method for plant endophyte bacteria, which comprises the following steps of: designing a specific primer for amplifying a plant endophyte bacteria absolute quantification internal reference sequence of a part matched with conserved sequences on two wings of an internal reference sequence; the method comprises the following steps: obtaining an internal reference sequence for amplifying absolute quantification of plant endophyte bacteria and increasing conserved sequences on two wings, and a specific primer for amplifying the plant endophyte bacteria 16SV5V7, mixing the series of internal reference sequences according to a certain gradient proportion, and jointly amplifying and building a library together with total DNA of a plant tissue sample, so as to obtain a specific primer for amplifying the plant endophyte bacteria 16SV5V7. The abundance of the endophyte flora in the plant tissue sample is absolutely quantified, so that the condition of quantitative distortion in plant endophyte detection is eliminated.

A cotton variety FM 765AX is disclosed. Seeds, plants, plant cells, plant tissue, harvested products and cotton lint as well as to hybrid cotton plants and seeds obtained by repeatedly crossing plants of variety FM 765AX with other plants are also provided. Plants and varieties produced by the method of essential derivation from plants of FM 765AX and to plants of FM 765AX reproduced by vegetative methods, including but not limited to tissue culture of regenerable cells or tissue from FM 765AX are provided.

The invention provides a chitosan-iron nanoparticle leaf surface resistance and control agent as well as a preparation method and application thereof, and belongs to the technical field of reduction of heavy metal pollution of crops. The preparation method comprises the following steps: dissolving chitosan in an organic solvent to obtain a chitosan solution, mixing the chitosan solution with ferrous sulfate, adding a sodium tripolyphosphate solution for crosslinking and stirring to obtain a solution containing precipitates, centrifuging, and collecting the precipitates to obtain the chitosan / ferrous sulfate composite material. The TPP and amino groups of the chitosan form ionic crosslinking through triphosphate groups, and Fe < 2 + > is successfully loaded on the surfaces of the chitosan nanoparticles. By complexing chitosan and Fe < 2 + >, the crystallinity of the material is reduced, and the adsorption activity of the amorphous structure is enhanced. Pot experiments show that by spraying the chitosan-iron nanoparticle leaf surface resistance and control agent on leaf surfaces, heavy metals of crops can be remarkably reduced, the antioxidant enzyme activity of plant tissues is improved, active oxygen is effectively removed, oxidative damage is relieved, and therefore plant growth is promoted, and the toxic effect of heavy metal stress on the crops is relieved.

The invention relates to the technical field of life science, in particular to a full-automatic nucleic acid extraction system and process, and the system comprises a complete machine frame, a sample adding and waste liquid treatment mechanism, an oscillation magnetic suction heating mechanism, a control system and a screen interaction system. The sample adding and waste liquid treatment mechanism realizes three-dimensional accurate positioning through transverse and longitudinal movement guide rails and a vertical movement assembly, comprises a large piston device and a small piston device which are connected in parallel, and can flexibly switch different volumes of pipettes and share a connector; the oscillation magnetic suction heating mechanism integrates oscillation, heating and magnetic suction functions and is matched with multi-specification suction heads and kits to complete sample treatment. According to the extraction process, all the mechanisms are linked through a control system, and full-flow operation such as sample cracking, solid-liquid separation, magnetic bead purification and elution is automatically completed. The nucleic acid extraction device realizes automation and precision of nucleic acid extraction, improves the operation efficiency and the extraction effect, and is suitable for nucleic acid extraction of samples such as plant tissues, animal tissues, blood and plasmids.

The invention discloses a protein extracting solution and a protein extracting method of polysaccharide and polyphenol plant tissues, and is characterized in that the protein extracting solution comprises the following components in molar ratio: 180-250 parts of trihydroxymethyl aminomethane hydrochloride buffer solution, 200-300 parts of sugar, 45-55 parts of chlorate, 10-70 parts of beta-mercaptoethanol and 0.5-2 parts of protease inhibitor. The browning condition of the total protein solution obtained by the protein extraction formula is obviously weakened, which indicates that the oxidation of polyphenol is obviously inhibited, the release of protein in a tissue sample is facilitated, and the extracted protein effect is better.

The invention provides an extraction method and application of natural plant polyphenol, which mainly utilizes a polylactic acid-polyethylene glycol copolymer to prepare an extraction solution for extracting and embedding polyphenol in natural plant tissues. The preparation method comprises the following steps: (1) preparing a PLA-PEG extraction solution; (2) extracting the natural plant polyphenol to obtain the natural plant polyphenol extract; and (3) carrying out post-treatment on the natural plant polyphenol extract. The invention provides a new path for high-valued utilization of agricultural and sideline products and industrial upgrading of natural polyphenol. The stability of the polyphenol is remarkably improved and the shelf life is prolonged through copolymer embedding, long-acting bacteriostasis is achieved, and the hydrogel has good biocompatibility and wide market prospects.

The utility model belongs to the technical field of tissue culture, and discloses a sterilization device for plant tissue culture, which comprises a box body structure and a sterilization assembly, the box body structure comprises a sterilization box, the sterilization assembly comprises a protection box and an infusion pump, the protection box is arranged at the top of the sterilization box, and the infusion pump is arranged in the protection box. The input end of the liquid conveying pump is connected with a liquid inlet pipe, the output end of the liquid conveying pump is connected with an output pipe, a plurality of connecting pipes are installed on one side of the output pipe, valves are installed on one sides of the connecting pipes, one ends of the connecting pipes are connected with flow dividing pipes, a plurality of branch pipes are installed on one sides of the flow dividing pipes, and a plurality of spraying heads are installed at the bottoms of the branch pipes. A high-temperature sterilization assembly is installed in the sterilization box and comprises a protection frame. The plant tissue culture sterilization device has the advantages that sterilization liquid is sprayed on the surface of plant tissue culture, bacteria, fungi or viruses are prevented from breeding on the surface of the plant tissue, the interior of the sterilization device is sterilized at high temperature, and the safety of plant tissue culture is improved.

The invention relates to the technical field of plant tissue extraction, in particular to an efficient extraction method and application of ginseng callus-derived exosomes, the extraction method comprises the following steps: S1, mixing ginseng callus with a PBS buffer solution, homogenizing and filtering to obtain a filtrate; s2, adding a protease inhibitor and a deep eutectic solvent into the filtrate, uniformly mixing, and standing to obtain a mixed solution; s3, centrifuging and filtering the mixed solution, and reserving supernate; s4, carrying out ultracentrifugation on the supernatant, collecting the precipitate, and resuspending with PBS to obtain a resuspension; and S5, further filtering the resuspension to obtain a filtrate, and storing at low temperature. The ginseng callus exosome extracted by the extraction method disclosed by the invention can be applied to preparation of drugs or cosmetics with anti-oxidation, anti-inflammatory and anti-aging effects, and the technical problems of low extraction efficiency, insufficient purity, complex extraction operation and poor stability of the extracted exosome in the existing exosome extraction technology can be effectively solved.

Chimeric GRF-GIF polypeptides that can be used to improve the capacity of plant cells to regenerate embryogenic plant tissues, plant organs, and whole plants are disclosed. Also disclosed are plant cells comprising the chimeric polypeptides and related methods for improving the capacity of the plant cells to regenerate embryogenic plant tissues, plant organs, and whole plants.

The invention provides a construction method of a plant tissue space transcriptome sequencing library, which comprises the following steps: fixing and embedding plant tissues to obtain embedded blocks; performing autofluorescence detection and tissue permeabilization treatment on part of the embedded blocks, and determining an autofluorescence threshold value and target tissue permeabilization time; slicing, pasting and fixing the remaining embedding blocks, and performing microscope fluorescence scanning detection and tissue permeabilization treatment on the tissue-containing chip according to an autofluorescence threshold value and target tissue permeabilization time to obtain a permeabilized tissue chip; and carrying out reverse transcription, tissue removal, cDNA release, recovery and amplification on the permeabilized tissue chip to obtain a cDNA amplification product. The plant tissue transcript constructed by the construction method is not easy to diffuse and high in capture rate, high-quality in-situ capture time-space group data can be obtained, the accuracy and the credibility are high, the sequencing accuracy is ensured, and the application value is high.

The invention discloses a fresh plant directed biotransformation method based on endogenous enzyme activation and a product and application thereof. The core of the preparation method is as follows: fresh plants (tuberous roots, stems or fruits) which are harvested in a ripe state and have metabolism in a dormant state (the respiration rate is lower than 5 mg CO / kg.h) are taken as raw materials, the raw materials are treated in an environment with the specific pressure being 0.02-0.20 MPa, the temperature being 70-121 DEG C and the humidity being 50-95%, a specific impregnating compound can be selectively combined, and the raw materials can be prepared into the plant culture medium. Under the conditions that exogenous microorganisms are not introduced and plant tissue structures are not damaged, the catalytic function of an endogenous metabolic enzyme system is safely and efficiently restarted and maximally promoted, and components of a plant body are driven to realize directional biotransformation. According to the process, conversion of primary metabolites and / or secondary metabolites is remarkably promoted, the content conversion of one or more beneficial components in the primary metabolites and / or the secondary metabolites is improved, and meanwhile, the biological and pharmaceutical structures and inherent metabonomics characteristics of the plants are completely reserved. The biotransformation product can be used as a high-quality raw material to be applied to medicines, common foods, health foods, special diet foods, daily chemical products and disinfection products, and can be further prepared into traditional Chinese medicine decoction pieces, solid preparations, semi-solid preparations and liquid preparations by adding or not adding pharmaceutically acceptable auxiliary materials.

A cotton variety FM 823AXTP is disclosed. Seeds, plants, plant cells, plant tissue, harvested products and cotton lint as well as to hybrid cotton plants and seeds obtained by repeatedly crossing plants of variety FM 823AXTP with other plants are also provided. Plants and varieties produced by the method of essential derivation from plants of FM 823AXTP and to plants of FM 823AXTP reproduced by vegetative methods, including but not limited to tissue culture of regenerable cells or tissue from FM 823AXTP are provided.

The invention discloses a radix gentianae callus cell culture and application method, and relates to the technical field of plant biology.The method comprises the following steps that in the laser irradiation process, a real-time spectrum analyzer is used for monitoring radix gentianae tissue, and absorption characteristic information of the plant tissue to light with different wavelengths is obtained. By combining the real-time spectral analysis and the machine learning model, the scheme can accurately monitor the interaction between the laser wavelength and the photosensitive component in the plant tissue, and by intelligently evaluating and dynamically adjusting the laser wavelength, the excessive absorption of laser energy is avoided, and the photochemical reaction and cell damage are reduced. The method not only reduces the potential damage of laser irradiation to plant cells, but also optimizes the synthesis process of medicinal components, and ensures the high efficiency, repeatability and reliability of callus culture. According to the scheme, powerful support is provided for improving the accuracy of experimental results and commercialized application of the gentiana scabra bunge.

The invention discloses a method for carrying out single-objective optimization on plant tissue culture based on artificial intelligence, and relates to the field of plant tissue culture, and the method comprises the following steps: building a plant tissue culture environment, and collecting and preprocessing environmental parameters; metabonomics data of plant tissues are measured through mass spectrum and liquid chromatography analysis, and a single-target optimization culture target is set in combination with environmental parameters; based on the environmental parameters and the metabonomics data, constructing a metabonomics analysis model through a random forest algorithm, and extracting a preliminary optimization path; according to the preliminary optimization path, using a sparse optimization algorithm to minimize plant culture resources, and obtaining a minimum resource scheme; plant tissue culture is carried out by using a minimum resource scheme, environmental parameters and metabonomics data are continuously collected, and metabonomics analysis model parameters are updated. By comprehensively utilizing artificial intelligence, metabonomics and an optimization algorithm, intelligence and precision of plant tissue culture and efficient utilization of resources are improved.

The invention provides an application of a CP gene, or a protein encoded by the CP gene, or a recombinant vector or host bacteria containing a CP gene coding region in down-regulation of the MeGRXC3 expression level. Researches find that CP and MeGRXC3 interact with each other, so that the growth ability of yeast in an SD / -Leu / -Trp / -His / -Ade auxotrophic culture medium can be improved, cell autophagy can be activated, the expression of MeGRXC3 can be down-regulated, and the active oxygen content of plant tissues co-expressed with MeGRXC3 can be increased. Research results lay a theoretical foundation for breeding of common cassava mosaic virus disease resistance.

The present invention relates to the field of genetic engineering technology, and specifically discloses a protein AaBBX21 for increasing the anthocyanin content of plants and the application of its encoding gene. The protein AaBBX21, whose gene encoding sequence is shown in SEQ ID No.1; after the encoding gene of AaBBX21 is overexpressed in plants by genetic engineering means, it is used to increase the anthocyanin content, ornamental value and edible value of plants. The present invention adopts genetic engineering technology to clone the AaBBX21 transcription factor from the succulent plant 'Halloween', and performs bioinformatics analysis, plant overexpression vector construction, Agrobacterium-mediated genetic transformation and phenotypic analysis of transgenic Arabidopsis thaliana on AaBBX21. The AaBBX21 gene identified by the present invention can significantly increase the anthocyanin content in plant tissues and the ornamental value and edible value of plants. The present invention lays a solid foundation for the subsequent use of genetic engineering technology to carry out transgenic precision breeding to improve the ornamental value and edible value of plants, and the AaBBX21 gene has great application prospects and market potential.

The invention belongs to the technical field of plant tissue culture, and particularly relates to an induction method of adventitious roots of cinnamomum camphora. According to the method disclosed by the invention, the adventitious root induction rate of the adventitious root of the cinnamomum camphora is remarkably improved by establishing a sterile system of the cinnamomum camphora, culturing cluster buds and sterile seedlings, promoting rooting of tissue culture seedlings, inhibiting browning, enabling the adventitious buds to extend and inducing the adventitious root through three ways, so that the problems of shortage of borneol resources and high market demand are solved; the method for inducing the adventitious roots of the cinnamomum camphora can be used for industrial production, and sufficient raw materials are provided for extraction of borneol; according to the method, large-scale culture of the adventitious roots of the cinnamomum camphora is achieved, a large number of pharmaceutical raw materials can be obtained at low cost in a short time, and an integrated production system can be formed with the pharmaceutical industry. The technology has important practical significance for promoting the development of the cinnamomum camphora industry, expanding the application of the pharmaceutical field, constructing a rare and endangered traditional Chinese medicinal material breeding technology system and realizing sustainable development and utilization.

The invention relates to a method for extracting genome DNA of plants rich in secondary metabolites and a buffer solution, and belongs to the technical field of molecular biology and botany. The method solves the technical problems of low DNA extraction efficiency, poor purity, easy degradation and the like when a traditional DNA extraction method is used for treating plant tissues rich in secondary metabolites such as alkaloid, polyphenol and the like. Comprising the following steps: adding a complexing agent such as polyethylene glycol or polyvinylpyrrolidone when grinding plant tissues in a liquid nitrogen environment; splitting by using a cell wall splitting buffer solution containing dithiothreitol and a nonionic surfactant; carrying out DNA release and extraction at 60-70 DEG C by adopting a CTAB (Cetyltrimethyl Ammonium Bromide) extraction buffer solution containing polyethylene glycol and papain; and then purifying and precipitating to obtain high-purity genome DNA (Deoxyribose Nucleic Acid). The method can effectively remove alkaloid, protein and other impurities, significantly improves the DNA yield and purity, and is suitable for genome sequencing, genetic resource protection, medicinal plant molecular identification and the like of plants with high secondary metabolites such as Stephania kwangsiensis and the like.

The invention discloses a mustard SNP (Single Nucleotide Polymorphism) molecular marker combination and application thereof. The mustard SNP molecular marker combination is composed of 1694 SNP molecular markers, and the physical positions of the 1694 SNP molecular markers are determined based on sequence alignment of a mustard reference genome Bju.gene. According to the scheme, the mustard SNP molecular marker combination marker is a co-dominant marker, and has the characteristics of high quality, single copy, high polymorphism, high specificity, high sensitivity and high resolution; the marking is not influenced by environmental conditions, seeds or any type of plant tissues can be used, the detection result is accurate, and the repeatability and the stability are good; different detection laboratories and different data results can be compared and verified, and the data has universal comparability, can be accurately used for background detection of mustard breeding materials, and has wide application universality.