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672 results about "Plant tissue" patented technology

Plant Tissue Definition. Plant tissue is a collection of similar cells performing an organized function for the plant. Each plant tissue is specialized for a unique purpose, and can be combined with other tissues to create organs such as leaves, flowers, stems and roots.

Multi-modal system and method for real-time plant hydration monitoring and irrigation management

The present invention discloses a multi-modal system for real-time plant hydration monitoring and irrigation management. The system comprises at least one acoustic sensor configured to emit controlled sound waves through plant tissues and detect corresponding vibrations and resonance frequencies using sensitive microphones or piezoelectric sensors to assess plant hydration. The system includes at least one bioelectrical sensor for monitoring bioelectrical signals. At least one nanotechnology-based sensor is configured to detect molecular changes in water content within plant cells through embedded or externally applied nanosensors. An aerial imaging device mounted on an unmanned aerial vehicle captures data related to canopy temperature, leaf color, and hydration indicators using infrared, multi-spectral, and thermal cameras. A central processing unit receives and analyzes data using predictive models, integrates the data via data fusion algorithm to generate a plant hydration profile, and controls water delivery through an irrigation management unit based on the hydration profile.
Owner:WU BRAD

Tissue culture and test-tube seedling acclimatization and transplantation method of rhodiola crenulata

The invention provides a rhodiola crenulata tissue culture and test-tube seedling acclimatization and transplantation method, and belongs to the technical field of plant tissue culture. The tissue culture method of the rhodiola crenulata comprises the following steps: (1) sterilizing rhodiola crenulata seeds, and performing germination culture to obtain adventitious buds; (2) transferring the adventitious buds obtained in the step (1) into an adventitious bud proliferation culture medium for adventitious bud proliferation culture to obtain cluster buds; and (3) transferring the cluster buds obtained in the step (2) into a seedling strengthening and rooting culture medium for seedling strengthening and rooting culture to obtain test-tube plantlets. The tissue culture and test-tube seedling acclimatization and transplantation method of rhodiola crenulata can provide a new method for large-area efficient cultivation of rhodiola crenulata, and has important application value for resource protection, development and utilization of rhodiola crenulata.
Owner:DEXING ACAD OF TRADITIONAL CHINESE MEDICINE EXPERIMENTAL TRAINING BASE +1

Insecticidal proteins compositions and methods of use

Disclosed herein are transformed plants, plant tissues, plant parts, plant cells, and plant seeds comprising a recombinant nucleic acid molecule encoding a polypeptide having pesticidal activity. Also disclosed herein are methods of protecting or treating a plant from infection by a plant pathogen or pest by transforming plants, plant tissues, plant parts, plant cells, and plant seeds with a recombinant nucleic acid molecule encoding a polypeptide having pesticidal activity.
Owner:GENECTIVE SA +9

Method for predicting nitrogen oxide infection change of plant based on AI image

The invention discloses a method for predicting nitrogen oxide infection change of a plant based on an AI image in the technical field of plant research, and the method comprises the steps: building a dynamic mapping relation of morphological indexes-infection concentration and time; the image morphology of the plant under time lapse and / or other concentrations is directly predicted by inputting the current image of the plant, the plant tissue does not need to be damaged, the whole-stage dynamic monitoring of the same plant from the health state to the pollution stress can be realized through regular image acquisition, the interference of traditional chemical sampling on the plant growth is avoided, and the monitoring accuracy is improved. The method is particularly suitable for rare plant or long-term ecological research, the abstract RGB value and the nitrogen oxide concentration are converted into visual results such as a color gradient diagram and a dynamic feature point evolution curve, non-professionals can rapidly judge the pollution level through color depth and patch distribution, and the environment early warning response time is shortened.
Owner:NANJING FORESTRY UNIV

Novel transcriptional regulator 30s ribosomal protein regulates physiological responses in plant cells

PendingUS20250137009A1HydrolasesPlant peptidesBiotechnologyRibosomal protein E-L30
The present disclosure provides genetically modified plants, plant cells, or plant tissues wherein the plants, plant cells, or plant tissues comprise an exogenous nucleic acid comprising a nucleotide sequence encoding a 30S ribosomal protein or a homolog thereof, wherein the 30S ribosomal protein or the homolog thereof is expressed in the plant, plant cell, or plant tissue. The current disclosure provides methods of improving drought tolerance and water loss in a plant, plant cell, or plant tissue, the methods comprising an exogenous nucleic acid sequence encoding a 30S ribosomal protein or a homolog thereof, wherein the 30S ribosomal protein or the homolog thereof is expressed in the plant, plant cell, or plant tissue. Additionally, the current disclosure is describes to genetically modified plants, plant cells, or plant tissues wherein the plants, plant cells, or plant tissues comprise an exogenous nucleic acid comprising a nucleotide sequence encoding a cation / H+ exchanger 20 (CHX20) protein or a homolog thereof, wherein the nucleotide sequence is operably linked to a heterologous promoter, and the CHX20 protein or homolog thereof is expressed in the plant, plant cell, or plant tissue. Finally, the current disclosure provides methods of improving drought tolerance and water loss in a plant, plant cell, or plant tissue, the methods comprising an exogenous nucleic acid sequence encoding a CHX20 or a homolog thereof, wherein the CHX20 or the homolog thereof is expressed in the plant, plant cell, or plant tissue.
Owner:UT BATTELLE LLC

Artemisia apiacea extracellular vesicle and preparation method thereof, pharmaceutical composition and medicine for treating cerebral apoplexy, and application of Artemisia apiacea extracellular vesicle in preparation of medicine for treating cerebral apoplexy

The invention discloses a preparation method of artemisia apiacea extracellular vesicles, which comprises the following steps: step 1, pre-treating artemisia apiacea, carrying out primary centrifugal treatment, removing large plant tissues and cell debris, carrying out secondary centrifugal treatment, filtering by a needle filter, and collecting; step 2, transferring the filtered suspension to a sucrose density gradient solution, then carrying out third centrifugal treatment, collecting the solution with the concentration of 30% in the layer, and carrying out fourth centrifugal treatment to obtain artemisia apiacea extracellular vesicles; the invention further discloses an artemisia apiacea extracellular vesicle, a pharmaceutical composition for treating cerebral apoplexy, a medicine and application of the artemisia apiacea extracellular vesicle in preparation of the medicine for treating cerebral apoplexy. The naturally-sourced vesicle delivery system provided by the invention is expected to have higher safety and lower toxic and side effects, and the enrichment concentration of the therapeutic component at the focus part of cerebral apoplexy is improved, so that the bioavailability and the treatment efficiency of the medicine are remarkably improved.
Owner:THE FIRST AFFILIATED HOSPITAL OF TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE

Plant paraffin embedding method

The invention relates to the technical field of plants, in particular to a paraffin embedding method for reducing plant RNA degradation. In the plant paraffin embedding method provided by the invention, a stationary liquid for fixing plant tissues is prepared from absolute ethyl alcohol and acetic acid in a volume ratio of (2-5): (1-4); buffer I used for dehydration is absolute ethyl alcohol and n-butyl alcohol with the volume ratio of (1-3): (1-2); buffer II used for waxing is n-butyl alcohol and low-temperature paraffin wax with the volume ratio of (1-3): (1-2), and the melting point of the paraffin wax is 20-50 DEG C. According to the method, low-temperature paraffin with the melting point of 20-50 DEG C is adopted, and a reagent for paraffin embedding and a paraffin embedding process are adjusted, so that the plant section with relatively high RNA integrity and relatively high tissue structure integrity is obtained. The paraffin embedding method provided by the invention is suitable for different types of molecular biology experiments of space transcriptome, immunohistochemistry, immunofluorescence and the like.
Owner:BIOMARKER TECH

Biochar material for repairing cadmium and tetracycline combined pollution in water body as well as preparation method and application of biochar material

ActiveCN120288751AOther chemical processesWater contaminantsPlant tissueSolanum sarrachoides
The invention relates to a biochar material for repairing cadmium and tetracycline combined pollution in a water body as well as preparation and application of the biochar material, and belongs to the technical field of water body pollution repairing. Comprising the following steps: (1) cleaning, drying and grinding plant tissues of solanum nigrum plants after the cadmium-polluted soil is repaired by fungi and solanum nigrum, so as to obtain solanum nigrum plant tissue powder; and (2) pyrolyzing the black nightshade plant tissue powder at 400-600 DEG C for 1-3 hours, cooling to 20-30 DEG C, and grinding to obtain the biochar material for repairing cadmium and tetracycline combined pollution in the water body. According to the invention, the plant tissue of the nightshade plant after the cadmium-polluted soil is repaired by the combination of fungi and nightshade is used as the raw material of the biochar material for the first time, so that the resource utilization of the polluted soil repairing plant is realized. The problem of plant treatment after polluted soil remediation is solved, consumption of extra resources is avoided, and the sewage treatment cost is greatly reduced.
Owner:SHANDONG JIANZHU UNIV

Diflufenican-containing compound and processing technology thereof

PendingCN119423101AGood defoaming and anti-foaming effectGood wetting and spreadingBiocideAnimal repellantsToxicologyDefoamer
The invention discloses a diflufenican-containing compound and a processing technology thereof, and relates to the technical field of agriculture. The diflufenican-containing compound agent is prepared from the following components in percentage by mass: 10 to 20 percent of diflufenican, 8 to 12 percent of isoproturon, 8 to 12 percent of florasulam, 0.5 to 1 percent of an anti-freezing agent, 0.5 to 1 percent of a de-foaming agent, 1.5 to 2.5 percent of a cosolvent, 1 to 3 percent of a wetting agent, 0.5 to 1 percent of a preservative, 1.5 to 3.5 percent of a dispersing agent and the balance of water. The defoaming agent is prepared from butynediol diethoxy ether grafted with low-hydrogen polydimethylsiloxane, and the defoaming agent is prepared from the butynediol diethoxy ether grafted with low-hydrogen polydimethylsiloxane; the dispersing agent is prepared from polyether amine bridging modified sodium lignin sulfonate. The pesticide is loaded on the nano silicon dioxide, so that the pesticide has good release performance and stability, the utilization rate of the pesticide is improved, the nano pesticide is small in size, the pesticide can better permeate into plant tissues, and a better prevention and treatment effect is achieved.
Owner:JIANGSU HEYUTAI CHEM CO LTD

Bulbophyte extracellular vesicle as well as preparation method and application thereof

The invention provides a bulb plant extracellular vesicle as well as a preparation method and application thereof, and belongs to the technical field of preparation of extracellular vesicles. The preparation method of the bulb plant extracellular vesicles provided by the invention comprises the following steps: S1, mixing plant tissues with a buffer solution, crushing, and filtering to obtain a plant stock solution; s2, treating the plant stock solution obtained in the step S1 to obtain a plant clear solution; s3, centrifuging the plant clear liquid obtained in the step S2 for 80-100 minutes under the conditions that the temperature is 3-5 DEG C and the weight is 110000-130000 g, retaining the precipitate, re-suspending the precipitate by using a buffer solution, and repeating for 1-2 times to obtain a re-suspension liquid; and S4, filtering the resuspension obtained in the step S3, and concentrating the filtrate to obtain the plant extracellular vesicles. The method can be used for efficiently extracting the extracellular vesicles from thick fresh lily and lycoris radiata tissues.
Owner:ZHEJIANG SCI-TECH UNIV

Methods and systems for depleting undesired cell culturing products from cell culture media

PCT designated stage expiredWO2025046586A1Tissue cultureBiotechnologyPlant tissue
A method of depleting undesired cell culturing products from a cell culture medium is provided. The method comprises contacting a cell culture medium which comprises lactate and ammonia with a plant tissue under conditions which allow depletion of the lactate and ammonia from the culture medium by the plant tissue, wherein the contacting is effected in an aseptic environment, thereby depleting undesired cell culturing products from the cell culture medium.
Owner:MEDIUM WELL TECHNOLOGICAL SOLUTIONS LTD

Plant exosome as well as extraction method and application thereof in cosmetics

The invention provides a plant exosome as well as an extraction method and application thereof in cosmetics, and belongs to the technical field of exosome extraction. The preparation method of the plant exosome comprises the following steps: mixing plant tissues with a solvent, carrying out high-pressure homogenization treatment, adding immobilized compound enzyme for enzymolysis, filtering, carrying out differential centrifugation on enzymatic hydrolysate, and carrying out size exclusion chromatography and ultrafiltration purification to obtain the plant exosome. Wherein exosome release and protection are considered, and the influence of agglomeration on the exosome extraction effect is avoided; high-pressure homogenization and immobilized compound enzyme enzymolysis are adopted, physical wall breaking is combined with chemical wall breaking, and the yield of the exosome is increased; the immobilized compound enzyme can avoid free enzyme residues, and the subsequent purification step is simplified; the plant exosome can be further purified through size exclusion chromatography and ultrafiltration. The exosome prepared by the method disclosed by the invention can retain the active ingredients of the exosome to the greatest extent, has excellent antioxidant, anti-inflammatory and whitening effects, and is suitable for the field of cosmetics.
Owner:GUANGZHOU GAOTAI BIOTECHNOLOGY CO LTD

Plant tissue-derived nanofibres

Provided herein are nanofibres including self-assembled cellular components derived from a homogenized plant tissue. Methods for preparing such nanofibres, as well as uses thereof in the treatment or prevention of diseases or disorders in a subject and / or as delivery vehicles are also described.
Owner:PSIGRYPH INC

Light formula generation method and system based on dendrobium officinale polysaccharide synthesis

The invention discloses a light formula generation method and system based on dendrobium officinale polysaccharide synthesis, particularly relates to the technical field of plant factory light environment regulation and control, and is used for solving the technical bottlenecks of polarization state instability and insufficient light signal penetration uniformity caused by environment interference in existing polarization state light regulation and control. A polarization state distortion area is sensed in real time based on Stokes parameters, physical interference factors of a reflection path and medium scattering are extracted, polarization state compensation parameters are generated through cross-correlation modeling, a laser light source direction angle and a phase delay amount are cooperatively optimized, and re-calibration of a distortion matrix is triggered through colorimetric method penetration uniformity verification; through closed-loop logic of physical interference traceability and dynamic compensation, the polarization stability of optical signals and the penetrability of plant tissues are synchronously improved, and efficient cooperation of a dendrobium officinale light response mechanism and a polysaccharide synthesis path is guaranteed.
Owner:SANMING UNIV +1

Gingko GbGRF2 gene and application of GbGRF2-GbGIF2 fusion gene thereof in plant tissue culture

The invention relates to the technical field of plant genetic engineering, in particular to a ginkgo GbGRF2 gene and application of a GbGRF2-GbGIF2 fusion gene of the ginkgo GbGRF2 gene in plant tissue culture. According to the invention, the GbGRF2 gene coding region full length of the GRF gene family of gingko is successfully cloned from gingko, and the gene presents a specific expression mode in the regeneration process of gingko callus; the overexpression of the fusion gene of GbGRF2 and GbGRF2-GbGIF2 can significantly promote the regeneration efficiency of adventitious buds in the in-vitro culture process of plants; the gene provided by the invention has important theoretical significance and application potential in a plant tissue regeneration regulation network, and plays a key role in improving in-vitro regeneration efficiency of gymnosperm.
Owner:NANJING FORESTRY UNIV

Movable crop stress in-vivo identification device and stress type identification method

The invention provides a movable crop stress in-vivo recognition device and a stress type recognition method, and the device comprises a fixed support which is provided with a supporting rod extending in the transverse direction; the transverse displacement rod extends in the longitudinal direction, is arranged on the supporting rod in a sliding mode and is used for moving on the supporting rod in the transverse direction; the longitudinal displacement platform is arranged on the transverse displacement rod in a sliding mode and used for moving on the transverse displacement rod in the longitudinal direction; and the Raman spectrum acquisition assembly is arranged on the longitudinal displacement platform and is used for acquiring a Raman spectrum on the target plant. According to the movable crop stress in-vivo recognition device and the stress type recognition method provided by the invention, the advantages of no need of sample pretreatment and high specificity of a Raman spectrum technology are utilized, stress response marker molecules in plant tissues are detected, and related information of a plant physiological state is obtained; and in-vivo, lossless and rapid identification of crop stress is realized by matching with structures such as a fixed bracket, a transverse displacement rod and a longitudinal displacement platform.
Owner:NONGXIN TECH BEIJING CO LTD +1

Plant endophytic bacterium absolute quantification method based on gradient internal reference

The invention discloses a gradient internal reference-based absolute quantification method for plant endophyte bacteria, which comprises the following steps of: designing a specific primer for amplifying a plant endophyte bacteria absolute quantification internal reference sequence of a part matched with conserved sequences on two wings of an internal reference sequence; the method comprises the following steps: obtaining an internal reference sequence for amplifying absolute quantification of plant endophyte bacteria and increasing conserved sequences on two wings, and a specific primer for amplifying the plant endophyte bacteria 16SV5V7, mixing the series of internal reference sequences according to a certain gradient proportion, and jointly amplifying and building a library together with total DNA of a plant tissue sample, so as to obtain a specific primer for amplifying the plant endophyte bacteria 16SV5V7. The abundance of the endophyte flora in the plant tissue sample is absolutely quantified, so that the condition of quantitative distortion in plant endophyte detection is eliminated.
Owner:GENESKY BIOTECH INC

Cotton variety FM 765ax

A cotton variety FM 765AX is disclosed. Seeds, plants, plant cells, plant tissue, harvested products and cotton lint as well as to hybrid cotton plants and seeds obtained by repeatedly crossing plants of variety FM 765AX with other plants are also provided. Plants and varieties produced by the method of essential derivation from plants of FM 765AX and to plants of FM 765AX reproduced by vegetative methods, including but not limited to tissue culture of regenerable cells or tissue from FM 765AX are provided.
Owner:BASF AGRICULTURAL SOLUTIONS US LLC

Chitosan-iron nanoparticle leaf surface blocking and controlling agent as well as preparation method and application thereof

The invention provides a chitosan-iron nanoparticle leaf surface resistance and control agent as well as a preparation method and application thereof, and belongs to the technical field of reduction of heavy metal pollution of crops. The preparation method comprises the following steps: dissolving chitosan in an organic solvent to obtain a chitosan solution, mixing the chitosan solution with ferrous sulfate, adding a sodium tripolyphosphate solution for crosslinking and stirring to obtain a solution containing precipitates, centrifuging, and collecting the precipitates to obtain the chitosan / ferrous sulfate composite material. The TPP and amino groups of the chitosan form ionic crosslinking through triphosphate groups, and Fe < 2 + > is successfully loaded on the surfaces of the chitosan nanoparticles. By complexing chitosan and Fe < 2 + >, the crystallinity of the material is reduced, and the adsorption activity of the amorphous structure is enhanced. Pot experiments show that by spraying the chitosan-iron nanoparticle leaf surface resistance and control agent on leaf surfaces, heavy metals of crops can be remarkably reduced, the antioxidant enzyme activity of plant tissues is improved, active oxygen is effectively removed, oxidative damage is relieved, and therefore plant growth is promoted, and the toxic effect of heavy metal stress on the crops is relieved.
Owner:GUILIN UNIVERSITY OF TECHNOLOGY

Full-automatic nucleic acid extraction system and process

The invention relates to the technical field of life science, in particular to a full-automatic nucleic acid extraction system and process, and the system comprises a complete machine frame, a sample adding and waste liquid treatment mechanism, an oscillation magnetic suction heating mechanism, a control system and a screen interaction system. The sample adding and waste liquid treatment mechanism realizes three-dimensional accurate positioning through transverse and longitudinal movement guide rails and a vertical movement assembly, comprises a large piston device and a small piston device which are connected in parallel, and can flexibly switch different volumes of pipettes and share a connector; the oscillation magnetic suction heating mechanism integrates oscillation, heating and magnetic suction functions and is matched with multi-specification suction heads and kits to complete sample treatment. According to the extraction process, all the mechanisms are linked through a control system, and full-flow operation such as sample cracking, solid-liquid separation, magnetic bead purification and elution is automatically completed. The nucleic acid extraction device realizes automation and precision of nucleic acid extraction, improves the operation efficiency and the extraction effect, and is suitable for nucleic acid extraction of samples such as plant tissues, animal tissues, blood and plasmids.
Owner:BEIJING QINGKE BIOTECHNOLOGY CO LTD

Protein extracting solution of polysaccharide and polyphenol plant tissues and protein extracting method

The invention discloses a protein extracting solution and a protein extracting method of polysaccharide and polyphenol plant tissues, and is characterized in that the protein extracting solution comprises the following components in molar ratio: 180-250 parts of trihydroxymethyl aminomethane hydrochloride buffer solution, 200-300 parts of sugar, 45-55 parts of chlorate, 10-70 parts of beta-mercaptoethanol and 0.5-2 parts of protease inhibitor. The browning condition of the total protein solution obtained by the protein extraction formula is obviously weakened, which indicates that the oxidation of polyphenol is obviously inhibited, the release of protein in a tissue sample is facilitated, and the extracted protein effect is better.
Owner:SHENZHEN UNIV

Methods of modulating gene expression in plants

The present invention provides a method for computer analysis of a genome of a plant subject, comprising identifying a plurality of sequences that collectively define a promoter set of the plant subject, where each sequence within the promoter set consists of an expression control region, the expression control region extends from an initiation codon of an open reading frame to about 100 kilobases away from the 5'and / or 3 'end of the initiation codon. A transcriptome in the form of mRNA expression data of a plant subject is obtained and analyzed using a sequence-based modeling algorithm to provide a predicted value of the expression level of each protein coding gene within the transcriptome and associate this value with the sequence of the corresponding expression control region within the promoter group. The method can be used to generate new designs for expression control sequences, which are most likely to provide the desired expression profile for operably linked coding sequences. Nucleic acid libraries, plant cells, plant tissues and plants comprising the novel expression control sequences are also provided.
Owner:PHYTOFORM LABS LTD

Extraction method and application of natural plant polyphenol

The invention provides an extraction method and application of natural plant polyphenol, which mainly utilizes a polylactic acid-polyethylene glycol copolymer to prepare an extraction solution for extracting and embedding polyphenol in natural plant tissues. The preparation method comprises the following steps: (1) preparing a PLA-PEG extraction solution; (2) extracting the natural plant polyphenol to obtain the natural plant polyphenol extract; and (3) carrying out post-treatment on the natural plant polyphenol extract. The invention provides a new path for high-valued utilization of agricultural and sideline products and industrial upgrading of natural polyphenol. The stability of the polyphenol is remarkably improved and the shelf life is prolonged through copolymer embedding, long-acting bacteriostasis is achieved, and the hydrogel has good biocompatibility and wide market prospects.
Owner:NORTHEAST FORESTRY UNIV

Sterilization device for plant tissue culture

The utility model belongs to the technical field of tissue culture, and discloses a sterilization device for plant tissue culture, which comprises a box body structure and a sterilization assembly, the box body structure comprises a sterilization box, the sterilization assembly comprises a protection box and an infusion pump, the protection box is arranged at the top of the sterilization box, and the infusion pump is arranged in the protection box. The input end of the liquid conveying pump is connected with a liquid inlet pipe, the output end of the liquid conveying pump is connected with an output pipe, a plurality of connecting pipes are installed on one side of the output pipe, valves are installed on one sides of the connecting pipes, one ends of the connecting pipes are connected with flow dividing pipes, a plurality of branch pipes are installed on one sides of the flow dividing pipes, and a plurality of spraying heads are installed at the bottoms of the branch pipes. A high-temperature sterilization assembly is installed in the sterilization box and comprises a protection frame. The plant tissue culture sterilization device has the advantages that sterilization liquid is sprayed on the surface of plant tissue culture, bacteria, fungi or viruses are prevented from breeding on the surface of the plant tissue, the interior of the sterilization device is sterilized at high temperature, and the safety of plant tissue culture is improved.
Owner:WEIKE (QINGDAO) BIOTECHNOLOGY CO LTD

Efficient extraction method of ginseng callus source exosome, exosome and application of exosome

The invention relates to the technical field of plant tissue extraction, in particular to an efficient extraction method and application of ginseng callus-derived exosomes, the extraction method comprises the following steps: S1, mixing ginseng callus with a PBS buffer solution, homogenizing and filtering to obtain a filtrate; s2, adding a protease inhibitor and a deep eutectic solvent into the filtrate, uniformly mixing, and standing to obtain a mixed solution; s3, centrifuging and filtering the mixed solution, and reserving supernate; s4, carrying out ultracentrifugation on the supernatant, collecting the precipitate, and resuspending with PBS to obtain a resuspension; and S5, further filtering the resuspension to obtain a filtrate, and storing at low temperature. The ginseng callus exosome extracted by the extraction method disclosed by the invention can be applied to preparation of drugs or cosmetics with anti-oxidation, anti-inflammatory and anti-aging effects, and the technical problems of low extraction efficiency, insufficient purity, complex extraction operation and poor stability of the extracted exosome in the existing exosome extraction technology can be effectively solved.
Owner:JIANGSU TONGCUI SCIENCE & TECHNOLOGY INNOVATION SERVICE CO LTD +1

Compositions and methods for soybean plant transformation

Chimeric GRF-GIF polypeptides that can be used to improve the capacity of plant cells to regenerate embryogenic plant tissues, plant organs, and whole plants are disclosed. Also disclosed are plant cells comprising the chimeric polypeptides and related methods for improving the capacity of the plant cells to regenerate embryogenic plant tissues, plant organs, and whole plants.
Owner:INARI AGRICULTURE TECHNOLOGY INC

Construction method and sequencing method of plant tissue space transcriptome sequencing library

The invention provides a construction method of a plant tissue space transcriptome sequencing library, which comprises the following steps: fixing and embedding plant tissues to obtain embedded blocks; performing autofluorescence detection and tissue permeabilization treatment on part of the embedded blocks, and determining an autofluorescence threshold value and target tissue permeabilization time; slicing, pasting and fixing the remaining embedding blocks, and performing microscope fluorescence scanning detection and tissue permeabilization treatment on the tissue-containing chip according to an autofluorescence threshold value and target tissue permeabilization time to obtain a permeabilized tissue chip; and carrying out reverse transcription, tissue removal, cDNA release, recovery and amplification on the permeabilized tissue chip to obtain a cDNA amplification product. The plant tissue transcript constructed by the construction method is not easy to diffuse and high in capture rate, high-quality in-situ capture time-space group data can be obtained, the accuracy and the credibility are high, the sequencing accuracy is ensured, and the application value is high.
Owner:SHENZHEN HUADA SANJIAN QIFA TECHNOLOGY CO LTD

Detection device for seeds and plant tissues

The utility model discloses a detection device for seeds and plant tissues. The detection device comprises a detection box and a seed sample feeder matched with the detection box, the detection box comprises a shell, a weighing module, a loading transparent plate, a camera module and a controller; an opening is formed in the top of the shell, the weighing module is fixed in the shell, the loading transparent plate is connected with the weighing module, the loading transparent plate is located at the opening in the top of the shell, the camera shooting module is located around the loading transparent plate, the camera shooting direction faces the loading transparent plate, and the camera shooting module and the weighing module are connected with the controller; the seed sample sending device comprises a loading plate, a sample sending plate and a partition plate, the loading plate comprises a plurality of seed hole sites, the seed hole sites are roughly the same as detected seeds in particle size and shape, the sample sending plate comprises a plurality of seed channels, and the seed hole sites correspond to the seed channels in position after the loading plate and the sample sending plate are stacked through a magnetic structure. A partition plate is inserted between the loading plate and the sample feeding plate. The seed metering device has the beneficial effects that the seed taking and metering processes are reduced, information such as weight, grain length, grain width and form can be obtained at one time, and the working efficiency is improved.
Owner:ZHONGKE HEFEI INTELLIGENT BREEDING ACCELERATOR INNOVATION RES INST CO LTD

Fresh plant directed biotransformation method based on endogenous enzyme activation and product and application thereof

The invention discloses a fresh plant directed biotransformation method based on endogenous enzyme activation and a product and application thereof. The core of the preparation method is as follows: fresh plants (tuberous roots, stems or fruits) which are harvested in a ripe state and have metabolism in a dormant state (the respiration rate is lower than 5 mg CO / kg.h) are taken as raw materials, the raw materials are treated in an environment with the specific pressure being 0.02-0.20 MPa, the temperature being 70-121 DEG C and the humidity being 50-95%, a specific impregnating compound can be selectively combined, and the raw materials can be prepared into the plant culture medium. Under the conditions that exogenous microorganisms are not introduced and plant tissue structures are not damaged, the catalytic function of an endogenous metabolic enzyme system is safely and efficiently restarted and maximally promoted, and components of a plant body are driven to realize directional biotransformation. According to the process, conversion of primary metabolites and / or secondary metabolites is remarkably promoted, the content conversion of one or more beneficial components in the primary metabolites and / or the secondary metabolites is improved, and meanwhile, the biological and pharmaceutical structures and inherent metabonomics characteristics of the plants are completely reserved. The biotransformation product can be used as a high-quality raw material to be applied to medicines, common foods, health foods, special diet foods, daily chemical products and disinfection products, and can be further prepared into traditional Chinese medicine decoction pieces, solid preparations, semi-solid preparations and liquid preparations by adding or not adding pharmaceutically acceptable auxiliary materials.
Owner:YUNNAN DECAITANG BIOMEDICAL TECH

Cotton variety FM 823axtp

A cotton variety FM 823AXTP is disclosed. Seeds, plants, plant cells, plant tissue, harvested products and cotton lint as well as to hybrid cotton plants and seeds obtained by repeatedly crossing plants of variety FM 823AXTP with other plants are also provided. Plants and varieties produced by the method of essential derivation from plants of FM 823AXTP and to plants of FM 823AXTP reproduced by vegetative methods, including but not limited to tissue culture of regenerable cells or tissue from FM 823AXTP are provided.
Owner:BASF AGRICULTURAL SOLUTIONS US LLC