782 results about "Callus" patented technology

The invention discloses a gene for improving wheat immature embryo transformation efficiency and application thereof, and belongs to the technical field of plant genetic engineering. According to the invention, a new wheat regeneration gene TaDJA gene is found, and the TaDJA gene is over-expressed in a wheat variety 'Kefeng No.3' which is extremely difficult to transform, so that the callus induction rate and the transformation efficiency of wheat immature embryos can be improved; the method is beneficial for integrating exogenous nucleic acid molecules into the genome of the wheat variety difficult to transform, and provides technical support for genetic improvement of the wheat variety difficult to transform.

The invention provides an AbAIL5 gene and application of the AbAIL5 gene in improvement of genetic transformation efficiency of amorphophallus bulbifer, and belongs to the technical field of biology. The invention provides an AbAIL5 gene. The nucleotide sequence of the AbAIL5 gene is shown as SEQ ID NO: 1. Genetic transformation is performed on the amorphophallus bulbifer somatic embryo by constructing an overexpression vector of the AbAIL5 gene, so that the genetic transformation efficiency of the amorphophallus bulbifer callus can be improved, and candidate genes are provided for application of a large-scale breeding technology and a genetic transformation technology of the amorphophallus bulbifer callus in good varieties.

The invention relates to the technical field of plant breeding, and particularly discloses a metabonomics-based alfalfa stress resistance gene analysis function and a breeding method. The core of the method is that screening is carried out by combining a tissue culture technology with metabolic intervention; an alfalfa explant is inoculated into an induction culture medium containing a specific metabolic pathway regulator for metabolic intervention culture; then transferring the obtained callus to a verification culture medium containing the metabolism regulator and an abiotic adversity stress factor at the same time, and carrying out metabolism mark verification and screening; and finally regenerating the screened cell line into a complete plant. According to the method, metabolic pressure is actively applied to the cellular level, and dual verification screening is implemented, so that early-stage, efficient and accurate breeding of the stress-resistant alfalfa material is realized, and the technical problems of long period, low efficiency and insufficient utilization of metabolic information in traditional breeding are solved.

The invention relates to the technical field of biology, in particular to a vitis davidii VdbHLH51 gene as well as an encoding protein and application of the vitis davidii VdbHLH51 gene. The vitis davidii VdbHLH51 gene is located on a chromosome 13 of vitis davidii and is distributed in a 772522-773497 region, and the nucleotide sequence of the vitis davidii VdbHLH51 gene is as shown in SEQ ID NO. 1. The amino acid sequence of the protein coded by the gene is as shown in SEQ ID No.2. The invention also discloses an application of the vitis davidii VdbHLH51 gene in improvement of resistance of grapes to pathogenic bacteria after overexpression of grape calluses. The VdbHLH51 gene provided by the invention can improve the resistance of grapes to grape anthracnose, and provides a theoretical basis for grape anthracnose resistance breeding.

The invention provides an exosome from rice as well as a preparation method and application thereof. The exosome has a double-layer membrane spherical structure, and the particle size range is 20-1000nm. The exosome is obtained from rice calluses, and has the characteristics of high biological safety, high stability and sustainable mass production. And tests prove that the exosome has the functions of resisting aging, promoting cell regeneration and tissue repair, protecting and the like, and also can remarkably improve the cell absorption efficiency and the delivery efficiency of active ingredients such as peptide cells.

The invention discloses a construction method and application of an in-vitro callus-like organ, the survival rate of the organ is improved by improving freeze-formed bio-ink, and a stable directional channel structure is further prepared, so that the internal mass transfer problem of the callus-like organ is improved, and the material exchange efficiency is improved. The forming mode of the callus-like organ is simple and effective, the stent material is easy to prepare, and the callus-like organ has mature cross-linking and printing characteristics, is expected to be applied to drug screening research or bone defect repair, is clear in process and simple in composition, and has relatively high operability.

The invention relates to the technical field of functional food and biological medicine, in particular to a modified pseudo-ginseng exosome and an intestinal conditioning gel composition and application thereof. According to the gel beverage, an exosome derived from panax notoginseng callus cultured in a laboratory is used as a core active component, is modified through phosphatidylserine-aminated pectin-genipin and is embedded into a hydrogel matrix composed of carboxymethyl chitosan and sodium alginate, so that the exosome is protected from being degraded in the stomach, and the activity of the exosome in the stomach is improved. The exosome can be effectively released under the intestinal pH and flora environment, so that the targeted delivery of the intestinal tract is realized, and the preparation method is suitable for improving the intestinal inflammation and maintaining the intestinal health. The invention can effectively solve the technical problems of uncontrollable raw material quality and safety, low oral delivery stability and active ingredient availability and poor medication compliance of the existing plant exosome.

The invention provides a dendrobium devonianum epiphytic tea tree cultivation method, which belongs to an epiphytic plant planting technology, and is characterized in that a dendrobium devonianum root system can be closely fused with a tea tree trunk through treatment of'minimal trauma + callus inducer ', and a structural foundation is laid for material exchange. The jasmonic acid methyl ester stress with a specific concentration is applied to the dendrobium nobile in the key phenological period, the dendrobium nobile is accurately induced to synthesize and secrete beneficial secondary metabolites, the theanine content in the tea can be remarkably increased, the proportion of ester type catechin can be reduced, and therefore the fresh degree of the tea is remarkably increased, and the astringent taste of the tea is remarkably reduced. The stress resistance of the dendrobium devonianum and the tea trees treated by the method is also enhanced, and the disease occurrence rate is reduced. While high-quality dendrobium nobile is harvested, the quality and economic benefits of the produced tea leaves are synchronously improved.

The invention discloses a method for enhancing squalene synthesis through oil tea HMGR gene editing. According to the method, phosphorylation regulation and control sites Ser47 and / or Thr208 recognized by MAPK are targeted, and enzymatic activity inhibition is relieved and metabolic flux is enhanced by constructing phosphorylation resistance mutants (such as Ser-> Ala and Thr-> Val). The constructed editing vector can be introduced into camellia oleifera tissues in manners of PEG-mediated protoplast transient transfer, agrobacterium infection of calluses and the like, so that stable expression is realized. Experiments prove that the enzyme activity of HMGR in the editor is remarkably improved, the squalene accumulation amount is increased by 50% or above, the editing efficiency is high, expression is stable, and the method is suitable for high-value development of plant triterpenoid functional components and excellent strain breeding. The method also has the potential of popularization and application in other triterpenoid synthetic crops.

The invention discloses a preparation method and application of a gynostemma pentaphylla exosome. According to the method, a high-activity callus is obtained by optimizing a callus induction culture medium, and a suspension culture system is established to realize biomass accumulation with the dry cell weight of 19.8 + / -0.7 g / L. A sequential progressive composite induction strategy (methyl jasmonate is added after chitosan is pre-induced for 24 hours) is innovatively adopted, so that the saponin content is remarkably increased to 102.6 + / -4.8 mg / g DW. Exosomes (P-Exos) are extracted from induced cells through an enzymolysis-magnetic bead affinity purification technology (WGA modified magnetic bead affinity capture after mild enzymolysis of a cracking buffer solution), the yield reaches 1.45 + / -0.08 mg / g cells, and the extraction efficiency is remarkably improved compared with that of a traditional gradient centrifugation method (C-Exos). Moreover, the gynostemma pentaphyllum exosome prepared by the invention can effectively improve the synthesis of skin collagen, has a better effect, can be applied to medicines for promoting skin wound repair or can be added into anti-aging cosmetics as a functional component, and has a very high application value.

The application discloses a grafting seedling cultivation method for improving grafting survival rate, relates to the technical field of grafting seedling cultivation, and aims at the problem of poor success rate of seedling grafting. The application deeply fuses plant physiological characteristics, geometric morphological parameters and grafting mechanics principles, constructs a quantifiable and traceable method selection model, improves the adaptation efficiency of the grafting method by more than 3 times, realizes continuous optimization of the healing environment through continuous circulation of data comparison and parameter adjustment, guarantees efficient grafting process, sets evaluation indexes such as callus development, new shoot growth, environmental stability and root development, accurately judges the growth quality of the grafting seedling at different healing stages, realizes continuous optimization of the healing environment through continuous circulation of data comparison and parameter adjustment, and guarantees efficient grafting process.

This invention relates to the field of plant genetic engineering technology, and more particularly to... SsNHX1 and SsSOS2 Dual-gene plant expression vector and its application in improving salt tolerance in alfalfa. The plant expression vector uses pCAMBIA1300 as its backbone and inserts genes derived from Suaeda salsa. SsNHX1 Genes and SsSOS2 It is constructed from genes. SsNHX1 Genes and SsSOS2 The CDS coding sequence of the gene is shown in SEQ ID NO:1 and SEQ ID NO:2 of the sequence listing. Using Agrobacterium tumefaciens EHA105-mediated transformation, the vector was introduced into the leaves of sterile alfalfa seedlings. Positive transgenic plants were obtained through co-culture, callus induction differentiation, and PCR identification. The advantage lies in the synergistic effect of the two genes, which effectively enhances the salt tolerance of alfalfa, enabling it to grow normally under 200–300 mM NaCl stress, providing a new solution for forage cultivation in saline soils.

The invention relates to an application of a genetic transformation method of populus sibirica and a CRISPR / Cas9 gene editing method in plant breeding, and belongs to the technical field of plant molecular breeding. The method comprises the following steps: (1) soaking an explant in an infection bacterium solution; (2) spreading on a CM1 culture medium; (3) transferring the explant to a CM2 culture medium for 4-5 weeks; (4) transferring the calluses to a CM3 culture medium until adventitious buds are obtained; (5) transferring the callus with the adventitious buds to a CM4 culture medium until cluster buds are obtained; and (6) cutting off clustered seedlings, and inserting the clustered seedlings on a CM5 culture medium. According to the application of the genetic transformation and CRISPR / Cas9 gene editing method of the poplar in plant breeding, a target gene is transferred into a poplar body to obtain a positive plant, meanwhile, a poplar phytoene dehydrogenase (PDS) gene is taken as an example, a poplar mutant of which the PDS gene is knocked out is successfully obtained by applying a CRISPR / Cas9 gene editing technology, and the method is suitable for plant breeding. And a technical support is provided for application of a CRISPR / Cas9 gene editing technology in molecular breeding of the populus sibirica.

The invention relates to the technical field of plant tissue culture and plant transgenosis, in particular to a method for co-transformation of an LcSUCLA alpha-LcSUCLA beta-pEGFC binary expression vector and Leymus chinensis double genes. The method comprises the following steps: obtaining leymus chinensis tissue culture sterile calluses, transforming agrobacterium competent by a freeze thawing method, activating agrobacterium tumefaciens, infecting the leymus chinensis calluses and co-culturing, screening and culturing plant transformation materials, differentiating and screening a culture medium and culture conditions, and differentiating and screening a culture medium and culture conditions as well as a rooting culture medium and culture conditions. According to the invention, double-gene co-transformation of leymus chinensis is realized, and a leymus chinensis double-transgenic plant is obtained. The cotransformation of the LcSUCLA alpha and the LcSUCLA beta in the leymus chinensis can synthesize more succinic acid than the single transformation, so that the tolerance of the leymus chinensis under the stress of basic salt is further improved.

The invention discloses a radix gentianae callus cell culture and application method, and relates to the technical field of plant biology.The method comprises the following steps that in the laser irradiation process, a real-time spectrum analyzer is used for monitoring radix gentianae tissue, and absorption characteristic information of the plant tissue to light with different wavelengths is obtained. By combining the real-time spectral analysis and the machine learning model, the scheme can accurately monitor the interaction between the laser wavelength and the photosensitive component in the plant tissue, and by intelligently evaluating and dynamically adjusting the laser wavelength, the excessive absorption of laser energy is avoided, and the photochemical reaction and cell damage are reduced. The method not only reduces the potential damage of laser irradiation to plant cells, but also optimizes the synthesis process of medicinal components, and ensures the high efficiency, repeatability and reliability of callus culture. According to the scheme, powerful support is provided for improving the accuracy of experimental results and commercialized application of the gentiana scabra bunge.

The invention relates to a key gene CbLTP63 for regulating and controlling salt stress response of catalpa bungei and application of the key gene CbLTP63, and belongs to the technical field of plant genetic engineering and biology. The nucleotide sequence of the CbLTP63 gene is as shown in Seq 1, and the coded amino acid sequence is as shown in Seq 2. The invention relates to cloning of a catalpa bungei salt stress response key gene CbLTP63. Identifying that the CbLTP63-GFP subcell is positioned on a cell membrane through an agrobacterium injection method; through qRT-PCR analysis and identification, the gene responds to salt stress on the transcriptional level and is down-regulated by H2O2, ABA and SA; through transgenosis identification, the gene is a catalpa bungei salt stress response negative regulation gene, and the salt tolerance of catalpa bungei calluses after gene silencing is obviously enhanced; the invention also relates to utilization of the gene in regulating stress response of catalpa bungei. The invention discloses a catalpa bungei salt stress response key gene CbLTP63, which has important application value in the fields of catalpa bungei gene engineering and clonal forestry.

The invention discloses an application of an NtMADS21 gene in regulation and control of tobacco traits. The nucleotide sequence of the NtMADS21 gene is shown as SEQ ID NO. 1. The invention also discloses an application of the NtMADS21 gene in regulation and control of tobacco traits. Through overexpression of the NtMADS21 gene, it is found that the NtMADS21 gene can synergistically improve the plant type and the tissue culture character of tobacco, specifically, the plant height is reduced, the number of leaves is increased to shape an ideal plant type, the leaf callus induction capacity is remarkably improved to 78.5%, in addition, the NtMADS21 gene has the effect of delaying flowering, and the yield of tobacco is increased. The invention provides a key gene resource integrating various excellent characters for genetic improvement and efficient breeding of tobacco.

The invention discloses application of a pear transcription factor PbrMADS1 to regulation and control of synthesis of stone cells and lignin as a component of the stone cells. A transcription factor PbrMADS1 gene separated from Dangshan pears belongs to SEP subfamily members, the nucleotide sequence of the transcription factor PbrMADS1 gene is shown as SEQ ID No.1, and the coded amino acid sequence of the transcription factor PbrMADS1 gene is shown as SEQ ID No.2 in a sequence table. The lignin content and the lignin synthetic gene expression quantity of the pear pulp callus with the PbrMADS1 being knocked out are remarkably reduced. Therefore, it is proved that the PbrMADS1 gene regulates formation of the pear fruit stone cells and the lignin component of the pear fruit stone cells. And the PbrMADS1 gene has the advantage of being capable of regulating and controlling a plurality of genes at the same time, so that a more efficient way is provided for molecular breeding. The discovery of the gene supplements and perfects a pear stone cell character transcriptional regulation mechanism, and provides a theoretical basis and gene resources for improving the pear fruit stone cell content.

The invention belongs to the technical field of biology, and particularly relates to a construction method and application of a tobacco plant for expressing rare ginsenoside. According to the method, dammarendiol synthase DDS, cytochrome P450 CYP716A47 and glycosyl transferase UGTPg1 / UGTPg45 are added into tobacco, heterologous synthesis of rare ginsenosides CK and Rh2 is achieved, dependence on traditional ginseng cultivation extraction is avoided, and cost is remarkably reduced and efficiency is remarkably improved. The characteristics of short growth cycle and easy culture of tobacco are utilized to overcome the problems of low efficiency and low yield of traditional synthesis; positive transgenic tobacco leaves are dedifferentiated to form callus cells, so that materials are provided for large-scale fermentation, and an industrial production foundation is laid.

This invention relates to the field of plant genetic engineering technology, and more particularly to... SsNHX1 and SsSOS1 A dual-gene plant expression vector and its application in improving salt tolerance in alfalfa. The plant expression vector uses pCAMBIA3301 as its backbone and inserts genes derived from Suaeda salsa. SsNHX1 Genes and SsSOS1 It is constructed from genes. SsNHX1 Genes and SsSOS1 The nucleotide sequences of the gene are shown in SEQ ID NO:1 and SEQ ID NO:2. Using Agrobacterium tumefaciens EHA105-mediated transformation, the vector was introduced into the leaves of sterile alfalfa seedlings. Positive transgenic plants were obtained through co-culture, callus induction differentiation, and PCR identification. The advantages are: the synergistic effect of the two genes effectively enhances the salt tolerance of alfalfa, enabling it to grow normally under 200–300 mM NaCl stress, providing a new solution for forage cultivation in saline soils.

The invention discloses a preparation method of aquilaria sinensis leaf exosomes and application of the aquilaria sinensis leaf exosomes in cosmetics, and belongs to the field of plant exosomes. The preparation method comprises the steps of explant selection and disinfection, inoculation and callus induction, suspension culture and exosome separation and purification. The aquilaria sinensis leaf exosome prepared by the preparation method can be applied to cosmetics; according to the method, through raw material preparation and process innovation, efficient transdermal and targeted delivery and a multi-component synergistic system, the effect bottleneck of a single component is broken through, and dual improvement of targeting and effectiveness is achieved.

This invention discloses a method for rapid seedling cultivation of Eucommia ulmoides via tissue culture, relating to the field of Eucommia ulmoides breeding technology. The method includes the following steps: using young, tender stem segments with axillary buds from superior Eucommia ulmoides strains as explants, after surface disinfection, the segments are cut into sections with axillary buds and inoculated into an axillary bud induction medium to induce axillary bud germination; the germinated axillary buds are cut into segments and inoculated into a specific medium for adventitious bud induction and elongation culture; the adventitious buds after proliferation culture are separated and subjected to a two-step in-bottle rooting method or external adventitious root induction, ultimately obtaining complete regenerated Eucommia ulmoides plants. Beneficial effects: This invention develops a simplified Eucommia ulmoides tissue culture propagation method that shortens the production cycle, has a high propagation coefficient, rapid rooting, high adventitious root induction rate, and minimal basal callus. It can rapidly obtain high-quality Eucommia ulmoides seedlings, which is of great significance for the large-scale propagation of superior strains and varietal improvement research, while also providing technical support for the sustainable development of the Eucommia ulmoides industry.

The invention discloses a leontopodium alpinum callus extract as well as a preparation method and application thereof, and belongs to the technical field of plant active ingredient extraction. The leontopodium alpinum callus extract is prepared by the following steps: mixing leontopodium alpinum callus freeze-dried powder with a buffer solution, adding cellulase, pectinase and beta-glucanase, uniformly mixing, reacting to obtain primary enzymolysis slurry, adjusting the primary enzymolysis slurry to be neutral, adding phospholipase A2, stirring, reacting to obtain deep enzymatic hydrolysate, filtering, washing, and drying to obtain the leontopodium alpinum callus extract. Transferring into an extraction kettle, adding an entrainer, and extracting to obtain a leontopodium alpinum extract; dissolving soybean phospholipid and cholesterol in ethanol to obtain an organic phase; dispersing the leontopodium alpinum extract in a buffer solution to obtain an active matter water phase; and injecting the organic phase into the active matter water phase, stirring, carrying out reduced pressure distillation, and homogenizing. The preparation method provided by the invention can efficiently and completely obtain the active component, and the nano-liposome formed by phospholipid molecules can effectively improve the stability of the active component.

The invention provides application of epiphyllum callus extract in preparation of cosmetics, and belongs to the technical field of new biological materials. Compared with a common epiphyllum extract, the epiphyllum callus extract provided by the invention is rich in high-content active ingredients, such as kaempferol, general flavone, total polyphenol, total protein and total sugar; the antioxidant skincare effect is achieved, and the removal efficiency of cells on DPPH free radicals can be improved. The skin care effect of promoting ATP synthesis is achieved, ATP synthesis can be remarkably promoted, and energy metabolism of damaged mitochondria is improved. Effective components of epiphyllum calluses are extracted, the effect of the epiphyllum calluses on human immortal keratinocytes is evaluated through cell experiments, it is proved that the epiphyllum calluses have excellent effects of resisting oxidation, resisting inflammation and repairing the keratinocytes, and the epiphyllum calluses are suitable for being used as raw materials to be applied to the cosmetic industry.

The invention relates to the technical field of plant grafting, in particular to a method for improving the grafting survival rate of malania oleifera, which comprises the following steps: S1, scion preparation: treating branches of excellent perennial wild malania oleifera plants in the field, conveying the branches to an inoculation field, and disinfecting to obtain disinfected branches; s2, stock preparation; s3, grafting: preparing a scion by using the disinfected branch, treating a stock, tightening the stock and a grafting film for tongue grafting of the scion, and smearing a healing agent on a cut of the cut stock and the upper end of the grafting film; s4, management after grafting. Compared with the prior art, the method has the advantages that superior plant branches and artificially cultivated stocks are subjected to tongue grafting, and compared with bud grafting, the yield time of grafted seedlings is shortened; and then the branch of the malania oleifera superior plant is treated immediately when the scion is collected, so that the problems that the activity of the branch is reduced in the transportation process and the formation of the callus of the stock and scion cambium is slow are solved, the grafting survival rate is increased, and the grafted seedling obtaining time is shortened.

The invention relates to the technical field of plant tissue culture, in particular to an anther in-vitro culture method for both highland barley and rice. The method comprises the following steps: S1, performing low-temperature refrigeration treatment on ears of naked barley / rice for 14-28 days; s2, performing disinfection and cleaning treatment on the Wheatstone to obtain anther in the Wheatstone; s3, treating the anther by using a pretreatment culture medium and an induction culture medium; s4, treating the callus or the embryoid obtained in the S3 by using a differential culture medium and a seedling strengthening and rooting culture medium; and S5, carrying out seedling hardening and doubling treatment on the obtained regenerated plants to obtain homozygous rice and naked barley seeds. According to the method, the problems of genotype limitation, low culture efficiency, high cost and the like in an anther culture technology are effectively solved, and the culture efficiency and practicability are improved.

The invention belongs to the technical field of agricultural biology, and particularly relates to a plant constitutive promoter OsSULTR2. 2pro and an application thereof. The promoter is characterized in that the promoter is OsSULTR2; the nucleotide sequence of the 2pro is as shown in SEQ ID NO. 1. The promoter provided by the invention is OsSULTR2; 2pro is a rice endogenous constitutive promoter, can drive a target gene to realize efficient and stable expression in plant callus, roots, stems, leaves, young ears, seeds and other tissues in a vegetative growth period, and can replace an existing non-plant-source promoter. The method has important application value in the field of plant genetic engineering, and can effectively reduce potential safety risks of transgenic plants caused by introduction of exogenous DNA.

The invention discloses a rubus cochinchinensis efficient genetic transformation method based on RUBY reporter gene and agrobacterium rhizogenes mediation, which realizes that the transformation period is shortened to be within 15 days and the transformation efficiency is improved to be more than 60% through direct explant treatment under a non-tissue culture condition. The method comprises the following specific steps: 1) preparing an explant material; (2) constructing an RUBY carrier and transforming agrobacterium rhizogenes; (3) infecting the explant by utilizing agrobacterium rhizogenes carrying a target gene; (4) co-culturing after infection and carrying out RUBY visual screening; 5) positive root molecular biology verification; (6) inducing positive roots into buds; according to the method, induction of the positive callus, the positive root and the positive bud of the rubus coreanus stem segment under the non-tissue culture condition is successfully achieved, a stable and efficient genetic transformation system is constructed, the method has the remarkable advantages of being easy and convenient to operate, low in cost, wide in applicability, high in reproducibility and the like, and a key technical support is provided for molecular breeding of the rubus coreanus.

The invention discloses a grafted sapling cultivation method capable of improving the grafting survival rate, relates to the technical field of grafted sapling cultivation, and aims to solve the problem that the sapling grafting success rate is poor. According to the method, a quantifiable and traceable method selection model is constructed by deeply fusing plant physiological characteristics, geometrical morphology parameters and a grafting mechanics principle, so that the adaptation efficiency of the grafting method is improved by more than three times, and continuous optimization of a healing environment is realized through continuously circulating data comparison and parameter adjustment; according to the method, high-efficiency grafting is guaranteed, growth quality of grafted saplings in different healing stages can be accurately judged by setting evaluation indexes such as callus development, shoot growth amount, environmental stability and root development, continuous optimization of healing environment is realized by continuously circulating data comparison and parameter adjustment, and the survival rate of the saplings is increased. And high efficiency of the grafting process is ensured.

The invention discloses a method and a device suitable for CUAMP. The invention discloses a method for extracting liriodendron tulipifera cell nucleuses by a Tag technology and application of the liriodendron tulipifera cell nucleuses. Hybridized liriodendron tulipifera calluses are used as materials, and protoplasts are released through enzymolysis solution vacuumizing and mild enzymolysis for 1.5 h; the method comprises the following steps: sequentially purifying by using a W5 solution and mannitol, detecting the activity by FDA, cracking by using an NE buffer solution, and washing by using a Wash buffer solution in two steps, thereby obtaining 2 * 10 < 6 >-3 * 10 < 6 > high-purity and high-integrity cell nucleuses. The obtained core is clean in background and complete in membrane structure, and can be directly used for CUTamp; tag is used for building a library, so that the magnetic bead capturing efficiency is improved by 46%, and protein-DNA interaction high-throughput sequencing under the condition of low sample size is realized. The method disclosed by the invention is simple and convenient to operate and good in repeatability, and provides key technical support for epigenetic research of rare tree species such as liriodendron tulipifera.