1413 results about "Callus" patented technology

The invention discloses an application of a peony PoAIL6 gene and an encoding protein thereof, and belongs to the technical field of biology. According to the invention, the peony PoAIL6 gene is cloned from a peony cultivar 'Paeonia ostii', and bioinformatics analysis is carried out. According to the invention, the peony PoAIL6 gene is transferred into arabidopsis thaliana, and phenotypic contrast finds that the number of lateral roots of the transgenic arabidopsis thaliana is significantly increased, which indicates that the overexpression of the peony PoAIL6 gene can promote the development of the root system of the arabidopsis thaliana. Arabidopsis thaliana roots are used for inducing calluses and carrying out callus differentiation, and it is found that the overexpressed peony PoAIL6 gene inhibits formation of the calluses and promotes the calluses to be differentiated into buds at the same time, which indicates that the peony PoAIL6 gene can regulate formation of arabidopsis thaliana somatic embryos. The invention provides a certain theoretical basis for establishing a complete plant regeneration and genetic transformation system of peony.

The invention provides an NAD < + > composition for skin anti-aging and firming cosmetics and a preparation process of the NAD < + > composition. The NAD + composition is prepared from the following components in parts by weight: 40 to 50 parts of dipropylene glycol, 0.1 to 1 part of nicotinamide, 0.001 to 0.01 part of nicotinamide adenine dinucleotide, 0.05 to 0.1 part of beta-nicotinamide mononucleotide, 0.1 to 0.5 part of leontopodium alpinum flower / leaf extract, 0.1 to 0.5 part of leontopodium alpinum callus extract, 0.1 to 0.5 part of leontopodium alpinum yeast fermentation lysate filtrate and 0.1 to 0.5 part of citric acid. The NAD + composition has the advantages that the preparation process is simple, the prepared NAD + composition has excellent synergy when being compared with some natural plant extract compositions, the effects of resisting aging and tightening skin can be better achieved, the NAD + composition can be effectively absorbed by the skin, the cell regeneration and differentiation capacity is promoted, the resilience force of the skin is helped to be improved, the skin is protected, and the skin is protected. The tightening and anti-aging effects are achieved.

The invention discloses a pennisetum purpureum gene PpbHLH148L and application thereof in improving the cold resistance of plants, and relates to the technical field of gene engineering. The nucleotide sequence of the gene is as shown in SEQ ID NO.5. After the gene is subjected to subcellular localization and expression condition analysis after cold stress, the gene is related to low-temperature tolerance. An overexpression vector of the gene is constructed, Agrobacterium tumefaciens is transferred, then Arabidopsis thaliana infection is carried out, cold tolerance determination is carried out on overexpressed Arabidopsis thaliana, phenotypic difference and physiological index difference between an overexpressed strain after cold treatment and a wild type are observed, and it is found that the gene can significantly improve the low-temperature stress tolerance of Arabidopsis thaliana. And in the low-temperature treatment of the pennisetum alopecuroides transgenic callus, the transgenic callus also shows higher tolerance than the wild callus at low temperature. The pennisetum purpureum gene PpbHLH148L can be used for low-temperature tolerance modification of plants, and has an application prospect in creation of low-temperature-resistant plant materials.

The invention discloses a construction method of an agrobacterium tumefaciens mediated masson pine genetic transformation system, and belongs to the technical field of genetic engineering. According to the construction method of the agrobacterium tumefaciens-mediated masson pine genetic transformation system, immature masson pine cones are collected, embryogenic calluses are induced and cultured, the embryogenic calluses of the masson pine in a PEM III stage are used as a material, suspension pre-culture is performed to obtain an acceptor material, and the acceptor material is subjected to genetic transformation to obtain the agrobacterium tumefaciens-mediated masson pine genetic transformation system. The method comprises the following steps: firstly, carrying out tissue culture on a transgenic somatic embryo, infecting by adopting an agrobacterium infection solution with an OD600 value of 0.2-0.6, carrying out co-culture, recovery culture and screening culture, carrying out histochemical staining by adopting GUS, carrying out molecular identification to obtain a transgenic embryogenic callus, and carrying out maturation treatment to finally obtain the transgenic somatic embryo. The masson pine genetic transformation system established by the invention is stable in transformation performance and high in transformation positive rate, and when an acceptor material is masson pine embryonic callus with the genotype of 1-3-5, the transformation positive rate is as high as 100%.

The invention relates to a same-variety grafting method for Tibetan pinus bungeana, and belongs to the technical field of forest vegetative propagation. In order to solve the technical problems that in the high-altitude environment, the grafting survival rate of the white bark pine is low, a connector is prone to being infected by microorganisms, and callus is formed slowly, efficient grafting is achieved through collaborative optimization of scion pretreatment and stock incision treatment. The method comprises the steps that a one-year-old branch with the diameter being 4-6 mm is selected and subjected to temperature and humidity control and composite antibacterial liquid dipping treatment; a sterile healing interface is formed in cooperation with rootstock incision slope cutting and four times of composite antibacterial liquid brushing; a one-year-old branch is cut, at least two or more bud points exist on the branch, two plump bud points are reserved, the branch is cut open from the position between the two plump bud points, and the scion is obtained. According to the method, the grafting survival rate and the interface stress resistance of the pinus bungeana in the alpine region are remarkably improved, the method is suitable for rapid breeding and hereditary characteristic retention of excellent germplasm of the pinus bungeana in Tibet plateau ecological restoration engineering, and more scion resources of the pinus bungeana can be utilized and protected.

The invention discloses an efficient agrobacterium tumefaciens-mediated euphorbia hirta genetic transformation method, and belongs to the technical field of plant genetic engineering. The method mainly comprises the following steps: 1) activation culture of agrobacterium tumefaciens; 2) seed disinfection and material selection; (3) inducing calluses; 4) preparation of an infection liquid: culturing agrobacterium in a YEB liquid culture medium, adding the agrobacterium into an MS liquid culture medium, adjusting the bacterial liquid until OD600 is 0.3-0.9, then adding 100 [mu] mol / L acetosyringone and 100 [mu] mol / L MgCl2, and shaking uniformly; the method comprises the following steps: (1) carrying out primary culture, (2) carrying out secondary culture, (3) carrying out secondary culture, (4) carrying out secondary culture, (5) carrying out infection, (6) carrying out co-culture, (7) carrying out cleaning and recovery culture, (8) carrying out screening differentiation and rooting induction, and (9) carrying out plant regeneration and authentication.Through the steps, a euphorbia hirta genetic transformation system can be successfully constructed, and the transformation rate of a positive plant obtained through transformation reaches 76.19%.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a method for establishing an elymus nutans seed callus induction and regeneration system. The invention provides a tissue culture medium and a tissue culture method for culturing elymus nutans, and in the tissue culture medium provided by the invention, 2, 4-D can induce explants to form calluses and promote cell division and growth. The CH has a good effect on differentiation of embryoid and adventitious buds. ABA can promote the development of seed embryos. The KT can promote the generation of calluses and the growth of adventitious buds. NAA can promote plant growth and promote generation of adventitious roots. Under the combined action of NAA, 2, 4-D, ABA, KT and CH, the callus induction rate of the mature embryo is improved. The invention also provides a regeneration tissue culture method of elymus nutans, the mature embryo is used as an explant for tissue culture, and the callus induction rate of the mature embryo is high.

The invention relates to the technical field of plant tissue culture, in particular to a Rhododendron pulchrum tissue culture method based on callus regeneration. According to the method, the tender cotyledons of aseptic seedlings are taken as explants, and an efficient Rhododendron pulchrum tissue culture and rapid propagation technical system is established through induction of calluses, subculture multiplication of the calluses, differentiation of adventitious buds, bud strengthening and rooting culture, so that complete tissue culture progeny plants are formed. The induction rate of the callus tissue of the system is 100.0%, the proliferation multiple of the callus tissue in a subculture cycle reaches 5.2 times, 28.5 adventitious buds can be differentiated from a callus tissue block with the size of about 0.5 mm * 0.5 mm * 0.5 mm, the rooting rate reaches 95.8%, the system is not limited by factors such as seasons, climate and regions, and efficient and rapid propagation can be achieved all the year round. Meanwhile, the establishment of the system also lays a technical foundation for genetic transformation improvement, mutant screening breeding and the like of the Rhododendron pulchrum based on the callus.

The invention relates to a preservation method and application of panax notoginseng germplasm resources, and relates to the technical field of panax notoginseng germplasm preservation. The preservation method comprises the following steps: (1) suspending pseudo-ginseng embryogenic calluses and a WPM culture medium, and screening and collecting to obtain pseudo-ginseng embryogenic cell clusters with the size of 0.45-1.5 mm; (2) transferring the panax notoginseng embryogenic cell cluster into a cryopreservation tube, and adding a loading solution for loading treatment to obtain a loaded embryogenic cell cluster; (3) carrying out vitrification treatment on the loaded panax notoginseng embryogenic cell cluster and a vitrification solution in a cryopreservation tube to obtain a vitrified panax notoginseng embryogenic cell cluster; (4) placing the cryopreservation tube filled with the vitrified pseudo-ginseng embryogenic cell cluster in liquid nitrogen for preservation; and after preservation, carrying out recovery culture on the pseudo-ginseng embryogenic cell cluster. According to the method, the survival rate reaches 90% after preservation, pseudo-ginseng plants can be rapidly differentiated, the differentiated pseudo-ginseng plants survive after being transplanted under natural conditions, the transplanting survival rate reaches 90%, and the pseudo-ginseng plants grow well in the later period.

The invention provides an elymus plant tissue culture method. The elymus plant tissue culture method comprises the following steps: (1) seed treatment; (2) callus induction; (3) carrying out callus subculture; and (4) callus differentiation. By selecting a proper seed treatment method, a callus selection standard and a culture medium used in each step, the method disclosed by the invention effectively improves the induction rate and the differentiation rate of the callus, solves the problems that the callus of elymus dahuricus and siberian wildrye is difficult to obtain and the differentiation rate is low when mature seeds are used as explants, and improves the survival rate of the elymus dahuricus and the siberian wildrye. A high-frequency plant regeneration system of the elymus dahuricus and the siberian wildrye is obtained.

The invention discloses a method for inducing sandalwood suspension cells to synthesize santalene. The method comprises the following steps: (1) performing induced callus culture on sandalwood explants; (2) carrying out suspension culture on the sandalwood callus to obtain a suspension cell line; and (3) adding cytokinin and auxin into the cell culture fluid of the suspension cell line, and performing induced culture to obtain the sandalwood suspension cell with high content of santalene. The method takes plant cell culture as a technical basis, and comprises the following steps: firstly, establishing a stable sandalwood suspension cell line; by adding cytokinin and auxin into the sandalwood cell culture fluid, the synthesis of the sandalene can be promoted, so that the content of the sandalene is increased. The result of the embodiment shows that according to the method provided by the invention, the biomass of the sandalwood suspension cells is improved by adding the cytokinin and the auxin. The product content in the sandalwood suspension cell line is about 3% and is obviously higher than that of a control group; gC-MS (gas chromatography-mass spectrometry) detection shows that the main components are alpha-santalene and beta-santalene.

The invention discloses a high-survival-rate method for grafting pinus sylvestris on pinus sylvestris in an alpine region. The grafting adaptability and the survival rate are improved through multi-dimensional innovation. A rising slope is arranged on the sunny side of a pinus sylvestris rootstock, double connectors of a falling slope are arranged on the shady side of the pinus sylvestris rootstock and matched with double wedge-shaped parts of Tibetan white bark pine scion buds, and the rootstock-scion contact face is increased; a photo-thermal response shape memory polymer bandage is adopted for fixation, germs are inhibited, and callus differentiation is promoted; a double-layer protection structure is constructed, a humidity control group is arranged in an inner-layer moisturizing breathable film to maintain the humidity of a connector to be larger than or equal to 60%, and an outer-layer anti-UV windproof net is intelligently adjusted to cope with sand wind and strong light. Furthermore, the callus differentiation is accurately regulated and controlled by pre-embedding pH responsive cellulose microspheres, or an intelligent temperature control film and an aerogel layer are additionally arranged to stabilize the temperature difference, and the freezing resistance is enhanced in combination with scion bud low-temperature domestication and abscisic acid treatment. The method remarkably improves the grafting survival rate, is suitable for efficient introduction of the Tibetan white bark pine in the alpine region, and is high in process synergy and high in environmental adaptability.

The invention discloses a large-scale preparation method of ginseng exosomes, which comprises the following steps: culturing calluses: inoculating explants into an induction culture medium until ginseng calluses are generated; proliferation of the calluses: inoculating the newborn calluses into a proliferation culture medium for proliferation culture; inducing germination and rooting: after the ginseng callus is amplified, transplanting the ginseng callus into a germination induction culture medium until the ginseng callus is germinated, and then transferring the ginseng callus into a rooting culture medium for culturing and rooting; and preparing the exosome, namely grinding root hairs grown from the ginseng callus regeneration plant, uniformly mixing the ground root hairs with PBS (Phosphate Buffer Solution) or performing liquid culture on ginseng suspension cells, and finally performing centrifugal treatment to obtain the ginseng exosome. According to the large-scale preparation method of the ginseng exosome, the endotoxin pollution in the growth and development process is reduced through ginseng callus culture rooting, and then the ginseng exosome is prepared, so that the large-scale preparation requirement in modern production and processing is met.

The invention relates to a method for producing notoginsenoside and ginsenoside by utilizing panax notoginseng cotyledon embryos, and belongs to the technical field of plant adventitious root tissue culture. The method comprises the following steps: inducing embryogenic calluses by utilizing immature zygotic embryos of panax notoginseng; proliferating the panax notoginseng embryogenic callus on a plate culture medium; s2, putting the pseudo-ginseng embryogenic callus obtained in the step S2 into a liquid culture medium, carrying out cell dispersion, and sieving by using a sieve with the aperture of 24-30 meshes; a mechanical stirring type bioreactor is filled with a liquid culture medium, and the globular embryo and the heart-shaped embryo are placed in the mechanical stirring type bioreactor to be cultured; s3, recovering the filtrate in the step S3, filtering by using a screen with the aperture of 30-80 meshes and 80-150 meshes to obtain uniform panax notoginseng embryogenic cells, and differentiating the panax notoginseng embryogenic cells in a triangular flask for the step S4; and drying the cotyledon embryo obtained in the step S4 at 45 DEG C, and grinding into powder. According to the method, large-scale generation of cotyledon embryos can be completed only in 4-6 weeks, the production process is remarkably accelerated, the method is suitable for industrial batch production requirements, and the production efficiency is high.

The invention discloses a gene for improving wheat immature embryo transformation efficiency and application thereof, and belongs to the technical field of plant genetic engineering. According to the invention, a new wheat regeneration gene TaDJA gene is found, and the TaDJA gene is over-expressed in a wheat variety 'Kefeng No.3' which is extremely difficult to transform, so that the callus induction rate and the transformation efficiency of wheat immature embryos can be improved; the method is beneficial for integrating exogenous nucleic acid molecules into the genome of the wheat variety difficult to transform, and provides technical support for genetic improvement of the wheat variety difficult to transform.

The invention discloses an application of an OsATS3 gene in regulation and control of rice flooding stress tolerance. According to the invention, an OsATS3 gene is constructed to edit a knockout vector, an agrobacterium tumefaciens-mediated method is adopted to transform rice calluses, and a knockout strain of the OsATS3 gene is obtained through screening, and the knockout strain of the OsATS3 gene shows a phenotype that after seeds germinate under a waterflooding condition, seedling root systems are developed, seedling growth of overground parts of seedlings is faster, the growth speed of first leaves is faster, and the seedling rate is high; the method has great significance in solving the problems of slow seedling growth, inhibited radicle development, low seedling rate and the like in the rice direct seeding process, and has a wide application prospect in plant breeding.

The invention discloses ribosomal protein PpRPL12 in Chinese pears and application of the ribosomal protein PpRPL12 in improving the disease resistance of plants. The ribosomal protein PpRPL12 with a positive effect on resisting pear black spot is screened from the Chinese pears. According to the invention, an overexpression vector and a virus silencing vector constructed by adopting the coding gene of the ribosomal protein PpRPL12 are used for verifying the pear black spot resistance function of the plant, and the result shows that the pear black spot resistance of the eggplant pear callus and the positive arabidopsis thaliana plant with the coding gene is obviously higher than that of the wild callus and the wild arabidopsis thaliana plant. After the ribosomal protein gene PpRPL12 in the pyrus betulaefolia seedling is silenced, it is found that the pear black spot resistance of the wild pyrus betulaefolia seedling is superior to that of the silenced pyrus betulaefolia seedling. It is proved that the ribosomal protein PpRPL12 and the coding gene thereof have important application value in the research of improving the pear black spot resistance of plants.

The invention relates to the technical field of plant genetic engineering, in particular to a genetic transformation method of prunus mume line plum blossom leaf calluses. The method comprises the following steps: firstly preparing a plum blossom genetic transformation receptor, then transforming agrobacterium tumefaciens by taking an RUBY gene as a reporter gene to prepare an infection bacterium solution, then carrying out callus infection and co-culture, and finally screening to obtain the resistant callus. According to the method provided by the invention, the resistant callus can be identified in an observation or PCR (Polymerase Chain Reaction) detection mode. The plum blossom callus is successfully infected, the red callus expressed by the RUBY reporter gene is obtained, the inductivity is high, and a good basis is provided for establishment and genetic improvement of a plum blossom genetic system.

The invention discloses a dendrobium officinale tissue culture and rapid propagation seedling raising method, which belongs to the field of biotechnology, and comprises the following steps: S1, explant treatment: selecting a dendrobium officinale plant with good growth vigor and no obvious pest and disease damage, carrying out high temperature stress treatment at 40-45 DEG C for 6-8 days, then taking the tender stem of the dendrobium officinale plant subjected to high temperature stress treatment as the explant, and carrying out tissue culture and rapid propagation on the explant; directly transferring the explant to a low temperature of 2-6 DEG C for stress treatment for 8-12 hours; s2, callus induction: taking the explant treated in the step S1, disinfecting the explant, taking the explant without axillary buds, cutting the explant into small blocks, inoculating the small blocks to a callus induction culture medium, and carrying out dark culture for 12-15 days; the callus induction culture medium comprises 1.5 to 3 mg / L of MS + 2, 4-D, 2 to 3 mg / L of KT and 0.1 to 0.3 mg / L of NAA; s3, bud differentiation induction and rooting induction. According to the tissue culture and rapid propagation method for the dendrobium officinale, the browning rate of the callus during callus induction can be effectively reduced, and the tissue culture seedling raising efficiency is improved.

The invention relates to the technical field of plant extraction, in particular to a preparation method of a plant cell exosome, the plant cell exosome and application of the plant cell exosome. The preparation method comprises the following steps: after the surface of a plant leaf is scratched, inoculating the plant leaf into a first culture medium for induction culture, then transferring the plant leaf into a second culture medium for subculture to obtain a callus, and then adding the callus into a buffer solution for grinding to obtain a filtrate; centrifuging the filtrate again to obtain supernate; and adding an exosome complexing agent into the supernate, incubating overnight, centrifuging, and freeze-drying to obtain the exosome. The first culture medium comprises an MS culture medium and the following components added into the MS culture medium: pullulan, 2, 4-D, agar and NAA; the second culture medium comprises an MS culture medium and the following components added into the MS culture medium: pullulan, 2, 4-D, agar, NAA and sodium phytate. The preparation method can increase the protein content in the exosome.

The invention discloses a method for efficiently promoting slash pine somatic embryo maturation, and relates to the technical field of plant breeding. According to the method, the slash pine embryogenic callus is inoculated into a somatic embryo maturation culture medium containing sulfonatin and / or activated carbon, or PEG8000 and / or chitosan oligosaccharide for somatic embryo induction culture, so that early maturation of slash pine somatic embryos can be remarkably promoted. Results of the invention show that when the concentration of the sulfokine added in the culture medium is 0.5 mg / L, the total number of mature somatic embryos is as high as 109 / dish; when 0.5 mg / L of sulfonatin and 1 mg / L of activated carbon are added at the same time, the number of mature somatic embryos is up to 288 / dish; when 120g / L of PEG 8000 and 6mg / L of chitosan oligosaccharide are added at the same time, the number of mature somatic embryos is up to 123 / dish. In conclusion, the method disclosed by the invention can efficiently promote slash pine somatic embryo maturation, and is high in somatic embryo induction efficiency and strong in practicability.

The invention belongs to the technical field of plant biology, and particularly relates to a genetic transformation method based on oat immature embryos, which comprises the following steps: preparing an infection solution containing a target gene; taking immature caryopses of the oats 21-23 days after pollination; infecting the immature embryos with infection liquid, and co-culturing the immature embryos after infection is completed; after co-culture is completed, immature embryos are sequentially transferred into an L3-T culture medium and an L3-B5 culture medium to induce formation of calluses, and after the calluses are formed, the calluses are sequentially induced to be differentiated, rooted and hardened. According to the method, efficient genetic transformation of exogenous genes is realized by selecting proper oat immature embryos, proper culture conditions and proper transformation conditions. By means of the genetic transformation method, the genetic transformation efficiency of the oats is improved to 25% from 2%-3%. And meanwhile, the immature embryo is used as a transformation acceptor material, and the complete plant can be efficiently and stably regenerated by the method disclosed by the invention.

The invention provides an AbAIL5 gene and application of the AbAIL5 gene in improvement of genetic transformation efficiency of amorphophallus bulbifer, and belongs to the technical field of biology. The invention provides an AbAIL5 gene. The nucleotide sequence of the AbAIL5 gene is shown as SEQ ID NO: 1. Genetic transformation is performed on the amorphophallus bulbifer somatic embryo by constructing an overexpression vector of the AbAIL5 gene, so that the genetic transformation efficiency of the amorphophallus bulbifer callus can be improved, and candidate genes are provided for application of a large-scale breeding technology and a genetic transformation technology of the amorphophallus bulbifer callus in good varieties.

The invention belongs to the technical field of plant genetic transformation, and particularly relates to a callus induction culture medium for genetic transformation, a culture medium combination and a genetic transformation method. The culture medium composition comprises a callus induction culture medium, a recovery culture medium, a screening culture medium, a differential culture medium and a rooting culture medium, wherein each 1L of the callus induction culture medium is prepared from the following components: 5-8g of tryptone, 5-8g of yeast, 100-120mg of sodium chloride, 0.8-1g of glutamic acid, 250-300mg of monopotassium phosphate, 100-150mg of magnesium sulfate, 0.8-1mu g of biotin, 10-20g of mannitol and the balance of water. By optimizing the culture medium formula and the transformation condition, the induction efficiency, the regeneration efficiency, the transformation efficiency and the plant regeneration capacity of the wheat are improved, and the method can be widely applied to the technical field of agricultural biology, especially in research, development and production of transgenic crops, and is beneficial to rapidly cultivating new varieties with excellent characters.

The invention discloses application of an OsWRKY28 gene in regulating and controlling the number of regenerated buds of rice callus, and belongs to the technical field of plant genetic engineering. According to the invention, a key regulation factor OsWRKY28 for regulating and controlling the regeneration of the rice callus is found. By constructing the rice OsWRKY28 knockout mutant and the overexpression strain, compared with a wild type, the number of regenerated buds of the callus of the OsWRKY28 knockout mutant is remarkably increased, and the number of regenerated buds of the callus of the OsWRKY28 overexpression strain is remarkably reduced. Therefore, it is determined that the OsWRKY28 gene plays an important role in regulating and controlling the regeneration process of the callus. Therefore, the regeneration capacity of the rice callus can be improved by knocking out the OsWRKY28 gene, so that the transgenosis efficiency is improved.

The invention relates to the technical field of plant breeding, and particularly discloses a metabonomics-based alfalfa stress resistance gene analysis function and a breeding method. The core of the method is that screening is carried out by combining a tissue culture technology with metabolic intervention; an alfalfa explant is inoculated into an induction culture medium containing a specific metabolic pathway regulator for metabolic intervention culture; then transferring the obtained callus to a verification culture medium containing the metabolism regulator and an abiotic adversity stress factor at the same time, and carrying out metabolism mark verification and screening; and finally regenerating the screened cell line into a complete plant. According to the method, metabolic pressure is actively applied to the cellular level, and dual verification screening is implemented, so that early-stage, efficient and accurate breeding of the stress-resistant alfalfa material is realized, and the technical problems of long period, low efficiency and insufficient utilization of metabolic information in traditional breeding are solved.

The invention discloses an efficient genetic transformation method of stem tumor sesbania, and belongs to the technical field of plant genetic transformation. According to the efficient genetic transformation method of the stem tumor sesbania, the cotyledonary node is used as the initial explant, callus induction is not needed, an optimized culture medium component is combined, a genetic transformation system of the stem tumor sesbania can be rapidly and effectively established, the genetic transformation efficiency can be improved and can reach 25% or above, the transformation time is shortened, and the cost is reduced. And a foundation is laid for domestication and accurate improvement work of the stem tumor sesbania.

The invention discloses a method for obtaining leymus chinensis mutants by using heavy ion beams to irradiate seed calluses, relates to the technical field of leymus chinensis breeding biology, and solves the three-low problems of low heading rate, low maturing rate and low germination rate of leymus chinensis seeds. After the leymus chinensis callus is subjected to induction culture, selecting faint yellow granular callus obtained by induction culture, and transferring the faint yellow granular callus into a culture dish filled with an induction culture medium for subculture; carrying out irradiation treatment by using a 5Gy carbon ion beam, transferring the callus subjected to irradiation treatment into a sterile culture bottle filled with a differential culture medium, and culturing to obtain adventitious buds; cutting off callus adhered to the base under a sterile condition, and inserting into a rooting culture medium for rooting culture after a rooting point is exposed; and after obvious root systems grow out, carrying out potting culture to obtain the leymus chinensis mutant. According to the invention, new genetic variation can be created, early in-vitro selection is realized, more effective mutants are obtained as new germplasm resources of leymus chinensis, and the breeding process of leymus chinensis is accelerated.

The invention discloses a method capable of improving the oat tissue culture efficiency. The method comprises the steps of oat seed disinfection, embryo taking, callus induction culture, callus subculture, callus differentiation culture, callus rooting culture, seedling hardening treatment, oat regeneration plant obtaining and the like. The method has the remarkable effects that by optimizing the proportion of hormones in the callus induction culture medium, the subculture medium, the differentiation induction culture medium and the rooting culture medium, the capability of performing dedifferentiation and redifferentiation on the oat embryos to form new plants can be effectively improved.

The invention relates to the technical field of biology, in particular to a vitis davidii VdbHLH51 gene as well as an encoding protein and application of the vitis davidii VdbHLH51 gene. The vitis davidii VdbHLH51 gene is located on a chromosome 13 of vitis davidii and is distributed in a 772522-773497 region, and the nucleotide sequence of the vitis davidii VdbHLH51 gene is as shown in SEQ ID NO. 1. The amino acid sequence of the protein coded by the gene is as shown in SEQ ID No.2. The invention also discloses an application of the vitis davidii VdbHLH51 gene in improvement of resistance of grapes to pathogenic bacteria after overexpression of grape calluses. The VdbHLH51 gene provided by the invention can improve the resistance of grapes to grape anthracnose, and provides a theoretical basis for grape anthracnose resistance breeding.