30 results about "Haworthia" patented technology

The invention provides a method for isolated culture of Haworthia succulent plants, which is characterized by comprising the followings steps of: (1) selecting and disinfecting explants; (2) building a sterile system; (3) cluster bud mulitiplication of the sterile system; (4) culturing rooting; and (5) exercising seedling of test-tube plantlet. The method utilizes tissue culture technology to carry out factorization rapid propagation on the tissue culture so as to have the advantages of less material consumption, rapid propagation, purification, rejuvenation, virus free and the like, the seedling propagation speed can be fasted, and annual production of high-quality seedlings is realized. The method has a positive meaning for development of flowers with the name.

The invention relates to a method for cultivating haworthia maughanii tissue. The method comprises the following steps of: preparing culture medium, selecting and sterilizing explants, callus induction, adventitious shoot induction, adventitious bud increment, strong seedling cultivation, adventitious bud root induction, somaclone domestication, and transplantation. The basic culture medium selects an MS culture medium; the dosage of saccharose is 20-40g/L; a coagulator is agar powder; the usage is 8-9g/L; the pH of the culture medium is adjusted to 5.7-5.8 before being charged; callus induction culture medium is 3.0-5.0mg/L of MS+6-BA and 0.05-0.2mg/L of NAA; adventitious shoot induction culture medium is 1.0-3.0mg/L of MS+6-BA and 0.05-0.2mg/L of NAA; adventitious bud increment culture medium is 0.5-1.0mg/L of MS+6-BA and 0.05-0.2mg/L of NAA; strong seedling culture medium is 0.1-0.2mg/L of MS+6-BA and 0.01-0.1mg/L of NAA; and root cultivation medium is 0-1.0mg/L of MS+IBA or 1/2MS + 0-1.0mg/L of IBA. The method has the advantages that the growth coefficient of the built rapid propagation technique system for haworthia maughanii tissue tissue cultivation is 2-2.5; the rooting rate is 100%; and the transplanting survival rate is over 98%.

The invention provides a special culture medium for improving the tissue culture propagation speed of haworthia succulent plants and a tissue culture method. The special culture medium is characterized in that the ingredients of the special culture medium include a basic culture medium and low-dosage 2,4-D. The tissue culture method comprises the following steps of 1 explant selection and sterilization, 2 inoculation and aseptic strain establishment and 3 transfer and expanding propagation. The problem of low tissue culture propagation speed of the haworthia succulent plants can be solved, the propagation speed of the haworthia plants is remarkably improved, the cost of the whole tissue culture is reduced, and the marketing speed of products is improved.

The invention relates to a soilless culture method for haworthia dural belonging to the succulent. The soilless culture method is characterized by comprising the following steps of S1. providing aseptic seedlings combining with tissue culture; S2. moving the aseptic seedlings out from a laboratory, and field planting the aseptic seedlings in a container using sphagna as a fixing object; and S3. spraying a nutrient solution, and keeping the relative humidity of air in the planting area more than 80%. The nutrient solution contains 0.3 % of nitrogen, 0.4 % of phosphor, 0.2 % of potassium, 0.5 % of humic acid, 0.03 % of carbendazim or thiophanate methyl and the balance water. By adopting the soilless culture method, insect diseases can be reduced, and the speed of growth of the haworthia dural can be distinctly raised.

The invention relates to a Haworthia cooperivar. pilfera M. B. Bayer OB-1 tissue culture formula. A MS medium is used as a basic medium, 0.25-0.35mg of 6-BA, 0.25-0.35mg of KT, 0.05-0.15mg of NAA and 5-6g of cane sugar into each liter of the basic medium, pH of the mixture is adjusted to 6.0, the temperature of the mixture is adjusted to 22+/-3 DEG C and the illumination intensity is adjusted to 3000lux. The seedling obtained by the technical scheme has a high transplant survival rate and a good form and solves the technical problem that a vitreous body and a malformed seedling can be easily produced in tissue culture.

The invention provides a cultivation material of black fungus, and belongs to the technical field of cultivation materials used for edible fungus cultivation. High-pole glossy privet branches and leaves, pineapple peels, Chinese holly branches and leaves, passion fruit peels, macadamia nut shells and traditional Chinese medicine dregs are adopted as main raw materials, and bean powder, gypsum powder, quicklime powder, monopotassium phosphate, white sugar and vitamin C are adopted as auxiliary raw materials, and the cultivation material of the black fungus is prepared after conducting fermentation on the main raw materials and the auxiliary materials. The cultivation material of the black fungus is comprehensive and rich in nutrition and high in quality, further has certain antibacterial ability and sterilizing ability, can effectively inhibit sundry bacteria contamination, reduce generating of diseases and pests and promote achievement of high-yield and stable-yield planting of black fungus, and is free of pollution, free of contamination and free of residues.

The invention discloses a haworthia maughanii tissue culture and rapid propagation method based on an LED light source. The method comprises the following steps: carrying out primary culture on a plump haworthia maughanii mature leaf as an explant; selecting red and blue LED illumination for induced differentiation and subculture; and carrying out rooting culture and domestication and transplanting to obtain a haworthia maughanii tissue culture seedling with high quality and high survival rate. According to the method, the proliferation cycle can be reduced to 28 days, the proliferation timescan be increased to 6.4, and the obtained multiple shoots are great in quantity and good in quality. The vitrification rate is reduced to 15.6%, and the obtained regrowth seedling is large in window,dwarf and robust. The plant root system is robust and healthy and the transplanting survival rate reaches 100%.

The invention relates to a ground face washing soap with traditional Chinese medicines and effects of moistening skins and removing freckle. The ground face washing soap mainly comprises a mature cold soap and traditional Chinese medicine liquid decoction. The mature cold soap comprises, by weight, 100 parts of macadamia nut oil, 75 parts of sweet almond oil, 175 parts of olive oil, 75 parts of palm oil, 75 parts of coconut oil, 65 parts of sodium hydroxide, 365 parts of traditional Chinese medicine liquid and 15 parts of honey, and the traditional Chinese medicine liquid comprises, by weight, 15 parts of radix angelicae sinensis, 5 parts of radix notoginseng, 10 parts of rhizoma chuanxiong, 15 parts of radix achyranthis bidentatae, 10 parts of flos carthami, 10 parts of flos rosae rugosae, 10 parts of fructus hippophae and 20 parts of radix astragali. The ground face washing soap has the advantages that grinding procedures in late periods are additionally carried out on the manually ground soap after the cold-press soap is completely manufactured, the traditional Chinese medicine liquid with functions of moistening the skins and removing the freckle is added into the mature cold-press manual soap, heating is omitted in the integral procedures, and the traditional Chinese medicine liquid cannot be easily destroyed by strong alkali; the mature cold soap is manually sufficiently ground, accordingly, the finished soap is fine and smooth and is soft in wash feel, the skins can be cleaned and simultaneously can be moist, the ground face washing soap has functions of whitening and moistening the skins and removing the freckle, and skin pigmentation can be effectively improved.

The invention discloses a haworthia maughanii in vitro culture method. Leaf blades of haworthia maughanii Daoqi are used as explants; an in vitro rapid propagation method system of primary culture, successive transfer culture and rooting culture is built. By adopting the method provided by the invention, the leaf blades can be used as the explants; the material taking difficulty can be reduced; the problems of explant browning, death and the like in the bud induction process can be solved; the callus inductivity and the regenerated seedling growth condition are improved; caespitose shoots withgreat quantity and high quality are obtained; the multiplication time is reduced; the multiplication times are improved; no browning and no pollution exist; the regenerated seedlings are healthy andstrong; no vain growth exists; the factory production requirements are met.

The present invention discloses blood pressure-lowering ginger tea granules and a preparation method thereof. The granules are characterized in that the granules are made from the following raw materials in parts by weight: ginger 60-65, ginger leaves 14-15, Litchi chinensis flesh 10-11, bamboo shoots 9-10, soya-bean milk 30-32, glutinous rice 7-8, oat germs 6-7, Hippophae rhamnoides powder 4-5, Queensland nut kernels 12-13, purple sweet potato powder 5-6, roots of kudzu vine 2-3, flos chrysanthemi 1-2, eucommia leaves 1-2, burdock roots 2.4-3, mulberry leaves 2-2.3, oblongleaf garcinia fruits and barks 1-1.2, khas aletris herb 0.9-1, bamboo-charcoal powder 4-5 and nutrition additives 5-6. The ginger, a principal raw material, is processed with a pestle made of wooden fish stone (hollow ore containing limonite) so that selenium, zinc and other trace elements contained in the wooden fish stone can integrate into the granules, which enhances nutritional values of the granules. In addition, often drinking the granules can play a role in lowering blood pressure with the addition of the roots of kudzu vine, the flos chrysanthemi and other Chinese medicinal herbs.

The invention discloses a planting method for a Hawaii lawn. The planting method comprises the following steps of 1, debris removal, wherein all debris and all weeds on the ground of planting land arecleaned up; 2, rotary tillage and flatting, wherein the planting land is subjected to deep rotary tillage and soil turning by using a rotary cultivator, and then the planting land is flatted; 3, pesticide spraying, wherein a weed killer is uniformly sprayed to the surface of the soil layer, which is subjected to rotary tillage and flatting, of the planting land; 4, ditching, wherein the plantingland is ditched; 5, seeding, wherein grass seeds are placed in ditches and then totally covered with soil; 6, fertilization, wherein after the lawn grows 1.5-2.5 centimeters, fertilization and thinning are conducted; 7, soft compression, wherein soft compression is conducted by using a roller press, and the lawn is compressed flat; 8, pruning, wherein pruning is conducted by using a trimmer, and after being pruned to a proper length, the lawn is made to grow transversely. The planting method for the Hawaii lawn is simple in process, short in planting cycle and small in fertilizing amount. Thelawn planted by using the planting method is light green in color, high in coverage rate, high in flatness, resistant to tread and drought and high in survival rate.

A ginger candy body-warming macadamia nut is prepared from, by weight, 130-150 parts of macadamia nut, 15-20 parts of fresh ginger, 40-45 parts of brown sugar, 10-15 parts of red dates, 20-25 parts of green bean starch, 5-6 parts of poria cocos, 3-4 parts of radix astragali, 2-3 parts of radix bupleuri, 3-4 parts of rhizoma pinelliae and the like. According to the ginger candy body-warming macadamia nut, the macadamia nut is boiled to be tasty with green tea leaves, rose flowers and the like so as to have faint flower, fruit and tea aroma and is then baked through microwaves to be dry, the crispiness of the macadamia nut is increased, and the rancidity caused by oil and fat rancidification when the macadamia nut is in a high temperature can be avoided; through certain technological processing, the macadamia nut is wrapped by fresh ginger, brown sugar and the like, so the macadamia nut has a body-warming effect; the macadamia nut is matched with extracts of traditional Chinese medicines including poria cocos, radix astragali, radix bupleuri, rhizoma pinelliae and the like, so the macadamia nut is endowed with good body-warming and weight-losing functions and is particularly suitable for being eaten by females.

These collars, leashes and harnesses are to be made of Hawaiian print fabrics with accessories attached. These Hawaiian prints include all designs such as surfing, floral, fisheries, fruits, tapa, ancient artistry, etc. These fabrics include all types such as cotton, rayon, quilted, heavy grade cotton canvas, etc. These accessories include any and all types such as flowers, fishes, pineapples, coconut trees, turtles, pearls, rhinestones, lace, etc.

The invention discloses a colloid type growth substrate for haworthia cooperivar. The colloid type growth substrate comprises the following components in parts by weight: 100 parts of water, 41-45 parts of fresh cow milk, 5-15 parts of starch, 2-6 parts of agar, 2-5 parts of potassium humate, 0.5-3 parts of sodium chloride, 0.5-1 part of zinc sulfate and 0.02-1 part of boric acid. The colloid type growth substrate for haworthia cooperivar can replace soil to be used as the growth substrate, and not only can effectively stimulate rooting and strengthen roots to enable petals of haworthia cooperivar to be stronger, but also can enable the haworthia cooperivar to be crystal and clear.

The present invention discloses macadamia ternifolia kernel body building lonicera fragrantissima fruit dried bean curds. The dried bean curds are prepared from the following raw materials: macadamia ternifolia kernels, lonicera fragrantissima, soybeans, lactuca sativa, northeast rice, water, lucid ganoderma, roselle calyx, salvia japonica, mint leaves, daphne odora, and lettuce seed powder. A preparation method comprises the following steps: (1) fruit pulp is prepared; (2) mixed slurry juice is prepared; (3) coarse cereal fruit and vegetable slurry is prepared; (4) nutrient solution is prepared; (5) filtrate is prepared; and (6) the coarse cereal fruit and vegetable slurry, nutrient solution, filtrate and lettuce seed powder are mixed evenly, and the mixture is prepared into finished products of the dried bean curds. By introducing the macadamia ternifolia kernels, lonicera fragrantissima, lactuca sativa, northeast rice and other nutrients of the fruits, vegetables and coarse cereals into the accessory materials of the dried bean curds and at the same time adding the health-care and beneficial ingredients of the lucid ganoderma, lettuce seed powder, etc. the dried bean curds are unique in flavor, fragrant, sweet and refreshing, and rich in various nutritional ingredients of proteins, amino acids, trace elements, etc., and have relative good therapeutic and health-care effects.

The invention provides a planting method for Macadamia ternifolia. The method comprises the following steps: 1, establishing a windbreak belt: planting a ring of Populus euphratica seedlings within 1km outside a Macadamia ternifolia planting area, planting the seedlings for at least 3 layers, and allowing a distance between two adjacent Populus euphratica seedlings to be not less than 3 m; 2, establishing a moisture retention belt: planting moisture retention shrubs with a width of at least 10 m outside the Macadamia ternifolia planting area in the windbreak belt, and establishing a grid-shaped water channel in the Macadamia ternifolia planting area; and 3, planting Macadamia ternifolia seedlings: digging grooves in the grid of the grid-shaped water channel, filling every groove with 0.6kg of a composite fertilizer and 0.7 kg of water, drying the groove in the sun for 24 d, filling every groove with a layer of mixed clay, placing one Macadamia ternifolia seedling in every groove, filing the surrounding of the Macadamia ternifolia seedling with the mixed clay, and filling and leveling up every groove. The windbreak belt and the water retention belt established outside the Macadamia ternifolia planting area can ensure the growth environment of the Macadamia ternifolia and reduce the damages caused by wind and drought.

The invention discloses a cultivation method for a nutrient succulent haworthia cymbiformis. The method comprises the following steps that seedlings are placed in a shallow flowerpot with soil mixed by bone meal, sand and soil and moved into an indoor semi-shade place for cultivation, the indoor temperature is controlled at 20-25 DEG C, and the seedling period is 30 days; from the first day to thetenth day, carrot juice is adopted for watering the seedlings, and the indoor temperature is controlled at 20-23 DEG C; from the eleventh day to the thirtieth day, the seedlings are moved to a balcony for illumination, cheerful music is played during illumination, and carrot juice is adopted for watering the seedlings; from the thirtieth day to the fiftieth day, leaves of the succulent start to grow, the succulent is moved to the indoor semi-shade place, a warm light is adopted for illuminating the succulent, and lemon juice is adopted for watering the succulent; from the fifty-first day to the sixtieth day, the succulent is illuminated by the warm light, and a mixed solution of Chinese medicine residues and water is adopted for watering the seedlings. The succulent haworthia cymbiformiscultivated by the method are high in growth speed, fleshy in leaf and high in absorptivity.

The invention relates to the field of disease control, in particular to trichoderma hairy trichoderma YW411, a culture method, a microbial inoculum and application of the trichoderma hairy trichoderma YW411. The invention provides a trichoderma haworthia YW411, and the preservation number of the trichoderma haworthia YW411 is CGMCC (China General Microbiological Culture Collection Center) No. 23202. The trichoderma haworthia YW411 disclosed by the invention can be used for effectively preventing and treating fungal diseases of plants and is free from pollution to the environment.

The invention relates to the technical field of plant tissue culture, and particularly discloses a tissue culture medium for haworthia succulent plants. The culture medium comprises the following components including, by concentration, 10-20 g/L of gel, 15-35 g/L of sucrose, 0.7-1.4 mg/L of heteroauxin, 0.7-1.4 mg/L of 6-BA, 2-3 mg/L of ganoderma lucidum polysaccharide, and 220-270 mg/L of a mineral element additive. The culture medium disclosed by the invention can be used for carrying out tissue culture on the haworthia succulent plants, and has the advantage of increasing the number and length of rhizomes of the plants in the tissue culture process.

The invention discloses a haworthia truncata propagating method by test tube micro-cuttage. The method comprises the following steps that (1), a material is selected and disinfected; (2), under aseptic conditions, leaves without stripes of the disinfected haworthia truncata are removed, leaves with stripes are connected to a culture medium for inducing leaf blade bases to grow; (3), the induced haworthia truncata plantlets are connected to a root medium, the haworthia truncataplantlets are cultivated until the haworthia truncata plantlets grow to root seedlings with the diameter of 2.8-3.2cm;and (4), the haworthia truncataroot seedlings with good root systems and complete blades are subjected to adaptive seedling refining for 18-22 days in a seedling hardening shed, then the hardened rootseedlings are taken out and disinfected,the disinfected haworthia truncata seedlings are placed on a cool and ventilated place to be air-dried until the blades of the haworthia truncata group cultureseedlings wrinkle, and transplant can be conducted. The haworthia truncata can be successfully propagated.

The present invention belongs to the technical field of plant tissue culture fast propagation and germplasm resource preservation, and relates to a blue-green haworthia succulent plant (laworthia succulent plant lanjing) germplasm resource in-vitro preservation method. The blue-green haworthia succulent plant germplasm resource in-vitro preservation method comprises the following steps: preparingculture medium; preparing blue-green haworthia succulent plant germplasm resources; conducting in-vitro preservation; and conducting proliferation culture. The provided convenient and simple blue-green haworthia succulent plant germplasm resource in-vitro preservation method is low in preservation cost and simple in operation, and at the same time, concentration and storage conditions of the growth-inhibiting culture medium during the blue-green haworthia succulent plant germplasm resource in-vitro preservation are provided.