A kind of in vitro culture method of Maohan twelve volumes

An in vitro culture, twelve-volume technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve high browning rate and pollution rate, long flowering time, long explant cycle, etc. problem, achieve the effects of shortening the transfer time, robust regenerated seedlings, and reducing the difficulty of material collection

Active Publication Date: 2020-02-18
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the reports on the rapid propagation of Twelves in vitro tissue culture use plant scapes as explants. Although it is easy to obtain regenerated buds, in practice, it takes a long time for plants to grow from seedlings to flowering. This leads to a longer and more difficult period of obtaining explants; at the same time, in the in vitro induction culture, it is often cultivated under continuous strong light, and the transfer period is longer, usually one month; accompanied by a higher browning rate and pollution rate, which cannot meet the needs of the current market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The leaves of "Daoqi" in Mao Han Twelve Volumes were selected as explants. After aseptic treatment of the explants, the explants were inoculated on the primary culture callus induction medium, and placed in a suitable temperature, humidity and Cultivate under light intensity conditions to induce callus, and the specific steps are:

[0022] 1. First-generation culture: select the leaves of Maohan Twelve Volumes "Daozu" as explants, wash them with washing powder, rinse with running water for 1 hour, transfer the explants to the aseptic operating table, and disinfect them with 75% alcohol first. 10 seconds, rinse once with sterile water, then disinfect with 0.1% mercuric chloride for 5 minutes or 15% sodium hypochlorite for 15 minutes, then rinse with sterile water for 4 times, cut off the base of the leaves and cut them into 1cm 2 Small pieces of each bottle were inoculated on the callus induction medium. The callus induction medium is MS medium, and its components are 6...

Embodiment 2

[0027] The leaves of "Daoqi" in Mao Han Twelve Volumes were selected as explants. After aseptic treatment of the explants, the explants were inoculated on the primary culture callus induction medium, and placed in a suitable temperature, humidity and Cultivate under light intensity conditions to induce callus, and the specific steps are:

[0028] 1. First-generation culture: select the leaves of Maohan Twelve Volumes "Daozu" as explants, wash them with washing powder, rinse them with running water for 2 hours, transfer the explants to the sterile operating table, and disinfect them with 75% alcohol first. For 20 seconds, rinse twice with sterile water, then disinfect with 0.1% mercuric chloride for 3 minutes, then rinse with sterile water, after 6 times, cut off the base of the leaves and cut them into 2cm 2 Small pieces of each bottle were inoculated on the callus induction medium. The callus induction medium is MS medium, the components are 6-benzylaminoadenine (6-BA) 2mg / L...

Embodiment 3

[0033] The leaves of "Daoqi" in Mao Han Twelve Volumes were selected as explants. After aseptic treatment of the explants, the explants were inoculated on the primary culture callus induction medium, and placed in a suitable temperature, humidity and Cultivate under light intensity conditions to induce callus, and the specific steps are:

[0034] 1. First-generation culture: select the leaves of Maohan Twelve Volumes "Daozu" as explants, wash them with washing powder, rinse with running water for 2 hours, transfer the explants to a sterile operating table, and disinfect them with 75% alcohol first For 30 seconds, rinse twice with sterile water, then disinfect with 0.1% mercuric chloride for 8 minutes, rinse with sterile water for 6 times, cut off the base of the leaves, and cut them into 1-2cm 2Small pieces of each bottle were inoculated on the callus induction medium. The callus induction medium is MS medium, and the components are 6-benzylaminoadenine (6-BA) 3mg / L, kinetin ...

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Abstract

The invention discloses a haworthia maughanii in vitro culture method. Leaf blades of haworthia maughanii Daoqi are used as explants; an in vitro rapid propagation method system of primary culture, successive transfer culture and rooting culture is built. By adopting the method provided by the invention, the leaf blades can be used as the explants; the material taking difficulty can be reduced; the problems of explant browning, death and the like in the bud induction process can be solved; the callus inductivity and the regenerated seedling growth condition are improved; caespitose shoots withgreat quantity and high quality are obtained; the multiplication time is reduced; the multiplication times are improved; no browning and no pollution exist; the regenerated seedlings are healthy andstrong; no vain growth exists; the factory production requirements are met.

Description

technical field [0001] The invention belongs to the technical field of asexual reproduction, and in particular relates to a method for in vitro cultivation of Mao Han Twelve Volumes, in particular to an in vitro cultivation method for Mao Han Twelve Volumes "Dao Wife". Background technique [0002] Mao Han Twelve Volumes (Haworthia maughanii), also known as Vientiane, the fleshy leaves obliquely protrude from the base and are arranged in a loose rosette shape. The leaves are dark green, gray-green or reddish-brown, with a rough surface. Maohan Twelve Juan likes a warm, dry and sunny environment. Not cold-resistant, afraid of high temperature and strong light, resistant to half shade. Prefers loose, well-drained sandy loam soil. The temperature in winter is not lower than 10°C. [0003] At present, most of the reports on the rapid propagation of Twelves in vitro tissue culture use plant scapes as explants. Although it is easy to obtain regenerated buds, in practice, it ta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王姗鲍华鹏张晓霞
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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