A disclosed
dendrobium officinale Kimura et Migo
tissue culture batch production method through one-step
seedling formation comprises the following steps: (1) breeding protocorm, namely, selecting a terminal
bud or an axillalry
bud of an excellent
dendrobium officinale Kimura et Migo
plant as an explant, sterilizing the explant, putting in a MS
agar solid medium with 6-BA concentration of 0.1-3.0 mg / L and KT concentration of 0.2-1.0 mg / L and IAA concentration of 0.1-1.0 mg / L and NAA concentration of 0.2-2.0 mg / L and
chlormequat concentration of 0.01-0.5 mg / L, inducing protocorm and obtaining a robust
bud through differentiation; (2) performing one-step
seedling formation
batch production, namely, directly transferring protocorm with the bud into an MS
agar solid medium with 6-BA concentration of 0.2-1.0 mg / L and IAA concentration of 0.2-0.5 mg / L and NAA concentration of 0.1-2.0 mg / L and
chlormequat concentration of 0.5-2.0 mg / L, and performing propagation, differentiation,
seedling strengthening and rooting; and (3)
transplanting the test-tube seedling. The provided
dendrobium officinale Kimura et Migo high-efficiency low-consumption
tissue culture method is short in breeding period and low in cost, is extremely suitable for industrialized production, and reaches industrialization level.