Dendrobium officinale Kimura et Migo tissue culture batch production method through one-step seedling formation

A Dendrobium candidum and tissue culture technology is applied in the field of one-step mass production of Dendrobium candidum tissue culture seedlings, which can solve the problems of high cost, cumbersome tissue culture procedures, and short reproduction cycle, and achieve high production efficiency, accelerated reproduction, The effect of simple method

Active Publication Date: 2015-12-16
花桓县精虹生物科技发展有限公司
View PDF9 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of cumbersome tissue culture procedure and high cost of Dendrobium candidum, the object of the present invention is to provide a tissue culture method of Dendrobium candidum with short propagation cycle and low cost with high efficiency and low consumption.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Dendrobium officinale Kimura et Migo tissue culture batch production method through one-step seedling formation
  • Dendrobium officinale Kimura et Migo tissue culture batch production method through one-step seedling formation
  • Dendrobium officinale Kimura et Migo tissue culture batch production method through one-step seedling formation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1. Rapid propagation of Dendrobium officinale by tissue culture.

[0035] 1. Culture medium preparation and sterilization

[0036] The first-generation medium is used for basic seedling propagation: MS+6-BA2.0mg / L+KT0.5mg / L+IAA0.25mg / L+NAA0.5mg / L+chlormequat 0.1mg / L

[0037] The subculture medium is used for one-step seedling mass production: MS+6-BA0.3mg / L+IAA0.3mg / L+NAA0.4mg / L+chlormequat 0.6mg / L.

[0038] The above medium was sterilized at 121°C for 20 minutes.

[0039] 2. Basic seedling propagation

[0040]Take the 0.5cm long stem section with terminal buds of Dendrobium officinale, first clean it with washing powder, wash it with sterile water for 5 to 7 times on a sterile operating table, soak it with 70% alcohol for 1 minute, and then put it into 0.1% L Sterilize in mercury solution for 8 to 12 minutes, rinse with sterile water for 5 to 6 times, transfer the terminal buds and axillary buds to the primary culture medium, and the number of explants in...

Embodiment 2

[0046] Embodiment 2, tissue culture rapid propagation Dioscorea shield leaf

[0047] 1. Culture medium preparation and sterilization

[0048] 2. Primary culture medium: MS+6-BA1.5mg / L+KT0.5mg / L+IAA0.3mg / L+NAA0.4mg / L+chlormequat 0.3mg / L;

[0049] 3. Subculture medium: MS+6-BA0.5mg / L+IAA0.2mg / L+NAA0.6mg / L+chlormequat 0.9mg / L

[0050] The above medium was sterilized at 121°C for 20 minutes.

[0051] Get Dendrobium officinale Kimura et Migo (Dendrobium officinale Kimura et Migo) terminal buds or stem segments, the basic operation method is consistent with Example 1; the effect is not much different from Example 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A disclosed dendrobium officinale Kimura et Migo tissue culture batch production method through one-step seedling formation comprises the following steps: (1) breeding protocorm, namely, selecting a terminal bud or an axillalry bud of an excellent dendrobium officinale Kimura et Migo plant as an explant, sterilizing the explant, putting in a MS agar solid medium with 6-BA concentration of 0.1-3.0 mg / L and KT concentration of 0.2-1.0 mg / L and IAA concentration of 0.1-1.0 mg / L and NAA concentration of 0.2-2.0 mg / L and chlormequat concentration of 0.01-0.5 mg / L, inducing protocorm and obtaining a robust bud through differentiation; (2) performing one-step seedling formation batch production, namely, directly transferring protocorm with the bud into an MS agar solid medium with 6-BA concentration of 0.2-1.0 mg / L and IAA concentration of 0.2-0.5 mg / L and NAA concentration of 0.1-2.0 mg / L and chlormequat concentration of 0.5-2.0 mg / L, and performing propagation, differentiation, seedling strengthening and rooting; and (3) transplanting the test-tube seedling. The provided dendrobium officinale Kimura et Migo high-efficiency low-consumption tissue culture method is short in breeding period and low in cost, is extremely suitable for industrialized production, and reaches industrialization level.

Description

technical field [0001] The invention belongs to the technical field of tissue culture of dendrobium candidum, and in particular relates to a method for mass production of dendrobium candidum tissue culture seedlings in one step. Background technique [0002] Dendrobium officinale Kimura et Migo is a perennial herb belonging to the family Dendrobium officinale. The medicinal and health value of Dendrobium candidum is very high. The term "Dendrobium candidum" comes from "Shen Nong's Herbal Classic". The international medicinal plant community calls it "the giant panda in the medicine world"; Modern clinical application and pharmacological research have confirmed that Dendrobium officinale has the magical effects of nourishing yin and moistening lungs, nourishing stomach and promoting body fluid, strengthening brain and improving eyesight, protecting liver and calming heart, clearing heat and reducing fire, nourishing fatigue of five internal organs, lightening body and prolong...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 段艳平余艳黄和芳
Owner 花桓县精虹生物科技发展有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap