735 results about "Dendrobium sanguinolentum" patented technology

The invention discloses a tree tissue culture method of dendrobium candidum, which belongs to the agriculture technical field, and aims at solving the technical problem that a tree tissue culture method of the dendrobium candidum is provided, and belongs to the bionics wild cultivation. In order to solve the problems, the tissue culture method of the dendrobium candidum comprises the following steps that: 1) a tree with surface of a tree bark having grains is selected as an epiphytic tree, a dendrobium candidum seedling after being domesticated is selected as an epiphytic seedling, and a hay band is selected as a binding rope; 2) the epiphytic seedling is arranged on the surface of a trunk of the epiphytic tree, and root of the epiphytic seedling is wound by the binding rope to be fixed; and 3) the tree tissue culture humidity is controlled at 70 percent to 85 percent. Due to the adoption of the method, the dendrobium candidum can be planted on the tree with the surface having the grains.

The invention provides a tie-to-tree dendrobium officinale cultivation method, comprising the steps of seedling tissue-culture breeding, tissue-culture seedling acclimatization and tie-to-tree cultivation of acclimatized seedlings. The step of tissue-culture seedling acclimatization comprises selection of bottled tissue-culture seedlings, selection and arrangement of a seedling acclimatization medium, selection of seedling acclimatization fertilizer, planting and after-planting management and is characterized in that the seedling acclimatization medium consists of hardwood sawdust and broken stone mixed in a mass ratio of 6.5-7.5:2.5-3.5, and the seedling acclimatization fertilizer is organic fertilizer prepared by fermenting herbivorous animal waste. The step of tie-to-tree cultivation of acclimatized seedlings comprises selection of acclimatized seedlings, selection of cultivation forest land, selection of variety of trees to be tied, seedling tying up and planting management. The tie-to-tree dendrobium officinale cultivation method provided by the invention has the advantages of low planting cost, easiness in management, high survival rate and long harvesting period; moreover, the harvest products have high quality.

The invention discloses a three-dimensional original ecological cultivation method for dendrobium candidum in forest. The three-dimensional original ecological cultivation method comprises the following steps of: firstly, selecting a cultivation environment; secondly, preparing a cultivation pot; thirdly, paving a matrix in the pot; fourthly, selecting seedlings; fifthly, permanently planting; sixthly, planting in a hanging manner or binding the seedling on the tree; seventhly, managing the cultivation; and eighthly, harvesting. The three-dimensional original ecological cultivation method disclosed by the invention provides a three-dimensional environment of original forest; the healthy growth of the dendrobium candidum is ensured; and the industrial and scale development of the dendrobium candidum can be realized; and the three-dimensional original ecological cultivation method disclosed by the invention has the advantages of high survival rate of seedlings, high yield, high quality of products, low cultivation cost and simpleness for management and operation.

The invention relates to a light control method for dendrobium officinale tissue culture, which comprises the following steps of: obtaining powdery seeds after dendrobium officinale fruits are sterilized, culturing the powdery seeds through a seed germination culture medium, and germinating the cultured seeds to form protocorms. The method is characterized in that: in the tissue culture process of dendrobium officinale seedlings, a light emitting diode (LED) plant growth illumination system is used as a light source, and different illumination treatment is implemented according to different stages of dendrobium officinale tissue culture. The different illumination treatment is implemented according to different growth stages so that the dendrobium officinale seedlings can furthest utilize light energy for photosynthesis and the highest growth amount is obtained. Experimental results show that: the culture period of the dendrobium officinale seedlings can be effectively shortened according to the technical scheme, the time from the seeds to the rooted finished seedlings is only 150 to 180 days, the growth period is shortened by 30 to 60 days compared with the culture under a common fluorescent lamp, and the seedlings cultured by the method have stable hereditary character and good quality.

The invention relates to a planting method of famous and precious Chinese herb medicine dendrobium officinale, and the method is characterized in that a cultivation bed of a plantation trough with an undulated bottom is established under a certain facility environment, multiple mixed organic matrixes mainly comprising pine bark are paved, a nondestructive planting technology for stretching tissue culture seedling roots is adopted, and scientific and reasonable dendrobium officinale planting method adopting comprehensive cultivation measures such as a photo and temperature control technology, a fertilizer control technology, an insect and harmful organism nuisanceless control technology and a sustainable harvesting technology is adopted in different growth seasons and for different plants according to the biological characteristics of the dendrobium officinale; and due to the adoption of the method, the ventilation, moisture evaporation and moistening functions of a plant root environment can be enhanced, and harmful substances influencing the growth of the plant can be eliminated in time, so that the plant can grow strong, active components are highly concentrated, the resistance is enhanced, a purpose of high yield and excellent quality can be realized, the high yield and stable yield of the dendrobium officinale can be guaranteed, the product quality is excellent, sustainability for harvesting the dendrobium officinale for multiple years can be realized, and super high yield of fresh products about 600kg per Mu every year can be realized after the method is put into production.

The invention discloses a vacuum freeze-drying processing method of dendrobium officinale, comprising the following steps of: (1) washing and draining fresh steam sections of dendrobium officinale and cutting into segments; (2) arranging the drained dendrobium officinale in a freeze-drying device for pre-freezing and constant-temperature freezing at 30 DEG C below zero to 35 DEG C below zero; (3)carrying out sublimation drying on the dendrobium officinale processed in the step (2); (4) heating the dendrobium officinale after the sublimation drying in the step for (3) for evaporating residualwater; and (5) packing the condensed dendrobium officinale products with water content of 6-9% dried in the step (4) into clean and sanitary food-grade aluminum-foil bags, sealing to the dendrobium officinale products. The dendrobium officinale products processed by the method disclosed by the invention are rich in dendrobium officinale polysaccharide, and can be directly eaten or taken as ingredients for stewing soup to eat or making tea to drink. The dendrobium officinale products keep original state, taste and pharmacological effects of the dendrobium officinale, can be stored and transported at normal temperature, and has more than five years of shelf life.

The invention provides a process for extracting dendrobium polysaccharide and dendrobine from dendrobium officinale. The raw material used in the process comprises dendrobium officinale, cellulase (a biochemical reagent), glucose, phenol, acetone, petroleum ether and absolute ethyl alcohol. According to the process provided by the invention, the polysaccharide and dendrobine in the dendrobium officinale are extracted by using cellulase-catalyzed extraction in a combination manner; the dendrobine is obtained when the dendrobium polysaccharide is obtained; high efficiency for single extraction of the polysaccharide by an enzymic method is kept; the effects of effectively breaking cell walls are improved; dissolution of polysaccharide and the like are promoted; the process and condition are mild; the extraction rate is improved to a certain extent; and the economic benefits are relatively high.

The invention discloses a preparation method of dendrobe health-care Pu'er tea cream and belongs to the field of food and drug technology. Different-specific-year Pu'er tea in 1 year, 5 years, 10 years and 20 years is used as a tea cream ingredient main body through reasonable proportioning, the mouth feeling advantages of tea in different specific years are sufficiently utilized, and the singleness of the mouth feeling of the Pu'er tea cream prepared by single raw materials is overcome, so the mouth feeling of the tea cream is purer and milder, and the fragrance is richer. In addition, polysaccharide effective ingredients of the dendrobe are extracted and are added into the tea cream at a proper proportion, the mouth feeling of the tea cream is enhanced, and meanwhile, the health-care function of the tea cream is enriched.

The invention discloses a processing method of tea making with dendrobium candidum leaves. The method comprises the steps of fresh leaf treatment, high-temperature withering, rolling, fermentation, drying and deodorizing, wherein in the high-temperature withering step, the treated fresh dendrobium candidum leaves are uniformly placed on a withering bed and continuously turned over for 3-5 hours at the constant temperature of 70-90 DEG C to allow the water content of the leaves to reach 60-70%, and the leaves are put away if no crackly fracture sound is heard when the leaves are caught up by hand and pinched forcibly. The method solves the problems that the existing dendrobium candidum leaves are wasted, tea prepared by the dendrobium candidum leaves is inconvenient to drink, and effective components are difficult to utilize; and the method has the advantages of being capable of making full use of a rare resource of dendrobium candidum, effectively increasing the leaching rate of medicinal components of dendrobium candidum, and facilitating brewing.

The invention discloses a dendrobium officinale stem cell and an isolated culture method thereof. The method comprises the following steps: culturing plant root tissue containing a quiescent centre, performing subcultring and scale-up culture on quiescent centre stem cell, and then preparing stem cell dry powder or extracting solution according to requirements. The dendrobium officinale stem cell overcomes the problem that dendrobium officinale in vitro material is easy to brown, the cell of a stem cell line is not differentiated, the cell increment is large in a nutritional condition, the culture time is short, and the cell activity is high. The dendrobium officinale stem cell and the method have the advantages that the technical process is easy to operate, the quantity of technological processes is less, the output is high, and the dendrobium officinale stem cell obtained is anti-aging and oxidation resistant, promotes hair growth and high baldness-resistant activity, and can be manufactured into functional health care products and cosmetics for preventing and curing various senescence and baldness and relevant Chinese drugs for preventing senescence and baldness.

The invention discloses a preparation method of a dendrobium officinale extract. The method comprises the following steps: by taking fresh dendrobium officinale strips as a raw material, performing fermentation with neutral protease after juice squeezing; quickly freezing fermentation liquor, naturally unfreezing the fermentation liquor, and removing starch and other impurities from the fermentation liquor; removing a starch solution through treatment with strongly acidic cation exchange resin and weakly basic anion exchange resin; and finally performing concentration with a titanium stick filter, performing alcohol precipitation and centrifugation, collecting supernatant, and freeze-drying the supernatant to prepare the dendrobium officinale extract. The preparation method is simple to operate, low in cost and high in industrialization degree. The dendrobium officinale extract provided by the invention is used for preparing medicines for treating wounds. Compared with a control group, the dendrobium officinale extract is remarkably different in wound size, healing speed and mean healing time within 10 days after operation of rabbits; and the dendrobium officinale extract can speed up the healing of a wound of a wound model rabbit.

The invention relates to a dendrobium candidum chewable tablet. The chewable tablet consists of dendrobium candidum fine powder and medicinal acceptable auxiliary materials, and is characterized in that: the particle diameter of the dendrobium candidum fine powder is 34-58 mum. The dendrobium candidum chewable tablet disclosed by the invention has high polysaccharide dissolution rate, and contributes to intestinal absorption of active ingredients; and the bioavailability of a preparation can be improved remarkably.

The invention relates to a method for preparing a dendrobium officinale culture substrate. The method specifically comprises the following steps: forming the culture substrate by using rotten woods, oil tea camellia cakes, camellia oleifera abel peels and corncobs according to quantities, wherein when the rotten woods and the corncobs are crushed, a crushing machine is placed with no screen, so that the rotten woods and the corncobs are crushed into crushed substances and irregular substances including pieces and blocks. The prepared substrate has the advantages as follows: the culture medium prepared by using the rotten woods as main raw material is very suitable for nutrients needed by dendrobium officinale during growing; by using a crushing measure of the rotten woods and the corncobs, the crushed substances can be transformed into nutrients for the dendrobium officinale to absorb; the pieces and the blocks are slowly transformed to follow-up nutrients; therefore, a long-acting and balanced nutrient supply system is formed; and as the blocks are distributed inside the substrate so that the substrate has fluffy texture, the substrate is also beneficial for more root surfaces of the dendrobium officinale to grow; therefore, the growing speed of the dendrobium officinale is accelerated and the yield of the dendrobium officinale is increased.

A dendrobium candidum planting method specifically includes the steps that dendrobium candidum seedlings are planted on tree species with longitudinal grooves in the surface of bark; before planting, the roots of the dendrobium candidum seedlings dip in a solid nutrient solution prepared through a high-water-absorbent material and a Jin Weiguo effective foliar fertilizer produced by the America Grow More Inc; during planting, the dendrobium candidum seedlings are bound on trunks through binding strips manufactured through shading nets so that the dendrobium candidum seedlings can be attached to the trunks. According to the method, the trunks serve as epiphytes, tress are used for shading, plastic greenhouses and other infrastructure do not need to be built, substrates are not used, cost is reduced by nearly a half, and environment is not influenced. Meanwhile, dendrobium candidum products cultivated through live trees are more ecological, higher in polysaccharide content and higher in quality. Dendrobium candidum is planted on the trees, does not occupy fertile farmland suitable for grains and does not occupy forest land suitable for the trees, and comprehensive benefits of the forest land can be better achieved. Most importantly, the live trees are planted in the wild, restoration of wild populations is facilitated, and sustainable development is achieved.

The invention discloses an industrial cultivation method for dendrobium candidum, belonging to the technical field of Chinese herbal medicine cultivation. The industrial cultivation method disclosed by the invention comprises the following steps of: taking freshly wild dendrobium candidum seeds as a raw material, thereby obtaining a dendrobium candidum sterile cultivating system; the industrial cultivation method comprises related technologies, such as seed treatment, seedling propagation, seedling tissue-cultivation and seedling training, transplantation and the like, so that the source of the dendrobium candidum seedlings and the geo-authentic character of the seedlings are effectively ensured; compared with the conventional seedling propagation, the seedling propagation speed is increased by 37%, the seedling training and transplantation survival rates are increased to above 90%, and the yield is increased by above 30%; and compared with the active ingredient content of wild dendrobium candidum, the dendrobium candidum, cultivated according to the method disclosed by the invention, comprises a same polysaccharide content as the wild dendrobium candidum, and slightly higher amino acid content as well as trace-element content than the wild dendrobium candidum.

The invention discloses a modified medium for improving propagation of stems of Dendrobium officinale and a propagation method. The medium comprises MS (Murashige and Skoog) basal medium, cane sugar 20g/l, agar 6g/L, 6-BA (benayl aminopurine) 2mg/l, NAA (alpha-naphthaleneacetic acid) 0.2-0.5mg/l, banana mash 15-20g/l, potato mash 15-20g/l, and common tap water replacing distilled water. The modified MS medium is used to propagate the stems with axillary buds of Dendrobium officinale, alkaloid and polysaccharide content of Dendrobium officinale is evidently increased, and Dendrobium officinale grow normally. By the method, consumption of cane sugar is reduced, the tap water is used to replace the distilled water, and accordingly tissue culture technology for Dendrobium officinale is more convenient, more efficient and resource-saving.

The invention relates to a flower tea manufacturing method, and in particular relates to a method for manufacturing dendrobium officinale flower tea. The method comprises the following steps: (1), collecting fresh dendrobium officinale flower, and subsequently removing impurities and cleaning; (2) removing water, namely, baking the fresh dendrobium officinale flower for 40-60 minutes at 120-125 DEG C, and subsequently spreading for cooling down at the room temperature; and (3) drying, namely, baking the dendrobium officinale flower with water removed for 30-50 minutes at 90-100 DEG C, highlighting the fragrance, drying, shaping and packaging in bags. The dendrobium officinale flower tea prepared by using the method is packaged and sealed in plastic bags or other packages, can be preserved for more than 1 year without color change or taste change at the temperature being about 4 DEG C, and is simple and convenient and high in practicability.

The invention discloses a normalized cultivation method for field cultivation of dendrobium officinale, and aims at providing a normalized cultivation method for field cultivation of dendrobium officinale which is high in product quality. The method comprises the first step of planting field selection, the second step of soil preparation, the third step of plastic tunnel build, the fourth step of matrix treatment, the fifth step of matrix laying, the sixth step of transplanting, the seventh step of field management and the eighth step of harvesting. The normalized cultivation method for the field cultivation of the dendrobium officinale is not only suitable for promotion cultivation in flat areas and gentle slope zones, but also suitable for the promotion cultivation in mountainous areas and hilly zones which are good in natural ecological environment. The influence of terrains on the cultivation method is small, the adaptability is strong, the cultivation method is suitable for promotion of artificial normalization and large-scale cultivation of the dendrobium officinale, the produced dendrobium officinale can meet the requirements of markets, and products with high quality can be provided for the markets.

The invention relates to a compound dendrobium candidum buccal tablet and a preparation method of the compound dendrobium candidum buccal tablet and aims at reaching the effects that the absorption and utilization efficiency and speed of the dendrobium candidum can be respectively improved and accelerated through the provided buccal tablet, and the provided preparation method has the characteristics that the process is simple, and the operation is convenient. The compound dendrobium candidum buccal tablet and the preparation method have the technical scheme that the compound dendrobium candidum buccal tablet comprises compound dendrobium candidum extract extractum, mannitol, dextrin and magnesium stearate which are mixed according to the weight proportion, and the compound dendrobium candidum extract extractum comprises dendrobium candidum, American ginseng, Chinese wolfberry fruit, rhizoma phragmitis, okra, mulberry and astragalus extracts which are determined according to the weight proportion. The preparation method of the buccal tablet comprises the following manufacture steps that (1) the materials are taken according to the raw material proportion; (2) the American ginseng is subjected to backflow extraction processing to obtain extractum A; (3) the extracted American ginseng medicine slag is mixed with other raw materials, decoction and extraction are carried out, and extractum B is obtained; (4) the extractum A and the extractum B are mixed and are then concentrated, and the compound dendrobium candidum extract extractum is obtained; (5) granulation is carried out; and (6) tabletting is carried out.

The invention discloses a method for planting dendrobium candidum in a wild imitating understory condition. The method comprises: selecting a wild imitating forest land; preparing soil and staking out; preparing sprouts; binding trees and planting; and managing the sprouts after planting. The method specifically comprises the steps: binding dendrobium candidum on artificial stubs by using a non-woven fabric as a bandage; and then putting the artificial stubs in an understory manner for natural growth. A light source required by growth of dendrobium candidum and the area of the forest land are effectively utilized for being combined with normalized culture management. As dendrobium candidum is planted by virtue of the method, dendrobium candidum is few in disease, is basically free of chemical fertilizers and pesticides and is relatively excellent in quality. The method has the advantages of being simple in operation, easy to master, strong in normalization, energy-saving, environmental-friendly and pollution-free and the like.

The invention discloses a dendrobium candidum jelly mask. The dendrobium candidum jelly mask is prepared from, by weight, 30-50 parts of Carbomer, 10-15 parts of water, 30-40 parts of moisturizer, 50-60 parts of dendrobium candidum extract, 5-10 parts of bletilla striata extract, 2-5 parts of white poria extract, 1-3 parts of radix sophorae flavescentis extract, 4-8 parts of aloe extract, 10-15 parts of seaweed extract and 5-8 parts of fructus forsythia extract. The dendrobium candidum jelly mask has the advantages that the dendrobium candidum jelly mask uses the dendrobium candidum as the main raw material and has effects of tonifying yin and replenishing moisture, resisting bacteria and diminishing inflammation, resisting oxidation, resisting aging and repairing damaged skin, the nontoxic moisturizer nonirritant to the skin is added, and the moisturizer is combined with the dendrobium candidum to enhance the beautifying effect of the dendrobium candidum.

The present invention provides a method for authenticating dendrobium officinale by using a HPLC fingerprint. The method comprises the following steps: (1) establishing a HPLC fingerprint of dendrobium officinale: a, precisely weighing powder of a dendrobium officinale medicinal material, adding water or a monohydric alcohol to carry out extraction and concentration, extracting by an organic solvent, carrying out treatments of recovering, concentrating, volume metering, filtering to obtain a tested sample solution; b, adopting a high performance liquid chromatography method to analyze the tested sample solution to obtain the fingerprint of the dendrobium officinale medicinal material; (2) establishing a standard fingerprint of the dendrobium officinale: carrying out analysis and comparison for 20 batches of the dendrobium officinale medicinal materials to obtain the standard fingerprint of the dendrobium officinale and the similarity of the dendrobium officinale medicinal material, wherein the similarity is more than 0.8; (3) adopting the same steps to detect the fingerprint of the dendrobium officinale counterfeit/substitute, carrying out comparison for the fingerprint of the dendrobium officinale counterfeit/substitute and the standard fingerprint of the dendrobium officinale, calculating the similarity, wherein the similarity is less than 0.6. According to the present invention, the standard fingerprint and the similarity can be adopted to effectively monitor the authenticity of the dendrobium officinale and the medicinal material quality.

The present invention relates to specially-made fertilizer for culturing dendrobium candidum wall.ex lindl, and a preparation method thereof, and belongs to the technical field of organic fertilizer. According to the present invention, the specially-made cultivation fertilizer suitable for the dendrobium candidum wall.ex lindl growth is developed according to the wild natural environment of the dendrobium candidum wall.ex lindl and the characteristic of endomycorrhiza of the dendrobium candidum wall.ex lindl; the formula of the specially-made fertilizer comprises tea bran, fish meal, bone meal, a small amount of phosphorus fertilizer and 100 parts by weight of water, wherein a ratio of the tea bran to the fish meal to the bone meal is 2-4:4-8:4-8. The preparation method comprises: concurrently pouring various components in a plastic bucket according to the ratio, uniformly mixing, fermenting for 3-6 months, and collecting supernatant after fermenting and ripening to obtain the specially-made fertilizer. With the present invention, the difficulties of low transplant survival rate of the dendrobium candidum wall.ex lindl seedlings, slow growth, not high yield and high cultivation cost are solved, such that the transplant survival rate of the dendrobium candidum wall.ex lindl seedlings is more than 98%, the dendrobium candidum wall.ex lindl flowers or produces stems and leaves 1-3 years in advance, the yield is high (the yield per mu is more than 250 kg), the active ingredient content is high, and the quality is excellent; the technical assurance is provided for rapidly culturing the dendrobium candidum wall.ex lindl; the active promotion effects are provided for development, utilization, and meeting of living requirements of the dendrobium candidum wall.ex lindl; important social benefits and economic benefits are provided.

The invention discloses a method for cultivating and planting organic dendrobium candidum. Illumination is conducted on dendrobium candidum tissue culture seedlings through LED red and blue hybrid light, and control and adjustment are conducted on factors such as an illumination period and a light intensity environment of the tissue culture seedlings at different stages, so that the speed of expanding propagation of the tissue culture seedlings is fast, and the yield is high; the cultivation time of the dendrobium candidum from seeds to finished rooting seedlings is about 160 days, high quality seedlings account for over 90% of total amount of the seedlings, the time for seedling hardening is about two weeks, the survival rate of seedbed planting in a greenhouse can reach over 98%, the time for seedling hardening for the tissue culture seedlings is short, the survival rate of transplanting is high, plant diseases and insect pests are few, the acidic water quality is adopted, fertilizer is not needed, and the growing environment of the dendrobium candidum is closer to the natural environment, so that the quality of the dendrobium candidum is guaranteed, nutritional ingredients of the dendrobium candidum is close to those of wild dendrobium candidum, and the medicinal value is high.

The invention discloses a pericarpium citri reticulatae, Pu'er and Se-enriched rice tea which comprises the following components by weight percent: 30-43 percent of hesperidium peels, 10-15 percent of Pu'er tea, 20-25 percent of Se-enriched rice, 10-13 percent of polished round-grained rice, 10-15 percent of dendrobium officinale, 2-5 percent of gingko seeds and 1-3 percent of ginger slices. According to the pericarpium citri reticulatae, Pu'er and Se-enriched rice tea, the pericarpium citri reticulatae internally filled with food materials such as Pu'er, Se-enriched rice, polished round-grained rice, the dendrobium officinale, the gingko seeds and the ginger slices with health care effects is used as a carrier, and used for making tea, and thus the pericarpium citri reticulatae, Pu'er and Se-enriched rice tea integrates the fragrance of the tea and the scorch aroma taste of the rice tea; according to the ingredients of the formula, the pericarpium citri reticulatae, Pu'er and Se-enriched rice tea can play a role in good health care when being drunk for a long time by old people, and has the advantages of improving the immunity and the ageing resistance capability of a user; the pericarpium citri reticulatae, Pu'er and Se-enriched rice tea also has functions of resisting cancer, reducing blood fat and blood pressure, resisting arteriosclerosis, regulating the qi flowing for strengthening the spleen, and the like, and is further suitable for being drunk by crowds of other age groups.

The invention relates to a method for producing dendrobium officinale tissue culture mycorrhizal seedlings, which comprises the following steps of: (1) culturing mycorrhizal fungi by using a solid or liquid culture medium, wherein the mycorrhizal fungi are mycorrhizal fungi LP1, namely Chaetomium sp.; (2) culturing mycorrhizal bacteria, wherein the mycorrhizal bacteria DP6 are Azotobacter sp.; (3) culturing the dendrobium officinale tissue culture seedlings; and (4) carrying out water culturing and training on the tissue culture seedlings. With the method for producing the dendrobium officinale tissue culture mycorrhizal seedlings, provided by the invention, not only the growth of the dendrobium officinale tissue culture seedlings is promoted, but also the tissue culture seedlings are obviously thickened. After transplantation, the survival rate can reach more than 95%, and the tissue culture seedlings have high growth rate, great bud tillering rate, rapid growth rate and strong stress resistance. Compared with the control group of dendrobium officinale seedlings which are not inoculated to mycorrhizal fungi and bacteria to perform tissue culture, the dendrobium officinale seedlings inoculated to mycorrhizal fungi and bacteria have more prosperous root systems, obviously inflated plant internodes and high fresh weight increased by 10-20%.