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Dendrobium officinale stem cell and isolated culture method thereof

A technology of separation and culture of Dendrobium officinale, applied in plant cells, skin diseases, pharmaceutical formulations, etc., can solve the problems of few successful cases of industrialization, and achieve the effects of short culture time, strong cell activity, and easy operation of technical processes

Inactive Publication Date: 2014-12-10
STEMCELL ESSENTIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, although there are also a lot of investment and efforts in plant cell culture, there are still very few successful cases of industrialization

Method used

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  • Dendrobium officinale stem cell and isolated culture method thereof
  • Dendrobium officinale stem cell and isolated culture method thereof
  • Dendrobium officinale stem cell and isolated culture method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The method for isolating and culturing stem cells of dendrobium officinale comprises the following steps:

[0049] (1) Induction and isolation of cell lines from the quiescent center of Dendrobium officinale

[0050] Collect the aseptic root tip containing the quiescent center of the Dendrobium officinale plant, remove the root cap, cut the 1mm length from the incision as an explant, put the explant into the N6 induction medium, and add 2mg / L2,4- D, the culture temperature is 25±1°C, and after 40 days of culture, the quiescent central stem cells are naturally separated from other root tissue cells due to their differences;

[0051] (2) Subculture and scale-up culture of Dendrobium officinale stem cells

[0052] 2-1 Subculture of Dendrobium candidum stem cells

[0053] Use the inoculation spatula to transfer the stem cells induced in step (1) into the liquid culture medium (N6 medium) in the flask, the pH value is 5.8, and cultivate in a controlled darkroom with a rota...

Embodiment 2

[0061] Effect of Dendrobium candidum stem cells on subacute aging mice induced by D-galactose

[0062] 1. Experimental materials:

[0063] 1.1 Drugs and reagents: Dendrobium candidum stem cell dry powder; superoxide dismutase (SOD) kit, 100T, Nanjing Jiancheng Bioengineering Research Institute, batch number: 20101009; glutinous glutinous peroxidase (GSH-Px) test kit, Nanjing Jiancheng Bioengineering Research Institute, batch number: 20101008; total antioxidant capacity (T-AOC) assay kit, 50T, Nanjing Jiancheng Bioengineering Research Institute, batch number: 20101013.

[0064] 1.2 Experimental animals: SPF grade KM mice, body weight (20±2) g, half male and half male, provided by the Experimental Animal Center of Guangzhou University of Traditional Chinese Medicine, certificate number: SCXK (Guangdong) 2008-0020.

[0065] 2. Experimental method

[0066] 2.1 Modeling: Take adult mice weighing 20±2g, half male and half male. The animals were randomly divided into 4 groups, nam...

Embodiment 3

[0074] The Effect Test on the Levels of Sex Hormone and Hepatocyte Growth Factor in Rats with Seborrheic Alopecia

[0075] 1. Experimental materials

[0076] 1.1 Drugs and reagents: Dendrobium candidum stem cell dry powder; testosterone propionate (Hubei Yuancheng Group Yuancheng Pharmaceutical Co., Ltd., batch number: 20100705); serum testosterone (ELISA kit), purchased from Guangzhou Qiyun Biotechnology Co., Ltd., production batch number E02T0007; Estradiol (E2) ELISA kit, purchased from Guangzhou Qiyun Biotechnology Co., Ltd., production batch number: E02E0023; hepatocyte growth factor ELISA kit, purchased from Guangzhou Qiyun Biotechnology Co., Ltd., production batch number : E02H0208; heparin sodium, Shanghai Yuanju Biotechnology Co., Ltd., production batch number: 20090124; Arowana peanut oil, Yihai Kerry Food Marketing Co., Ltd.; 0.9% sodium chloride injection, Shandong Lukang Chenxin Pharmaceutical Co., Ltd., Production batch number: 1004234202.

[0077] 1.2 Experime...

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Abstract

The invention discloses a dendrobium officinale stem cell and an isolated culture method thereof. The method comprises the following steps: culturing plant root tissue containing a quiescent centre, performing subcultring and scale-up culture on quiescent centre stem cell, and then preparing stem cell dry powder or extracting solution according to requirements. The dendrobium officinale stem cell overcomes the problem that dendrobium officinale in vitro material is easy to brown, the cell of a stem cell line is not differentiated, the cell increment is large in a nutritional condition, the culture time is short, and the cell activity is high. The dendrobium officinale stem cell and the method have the advantages that the technical process is easy to operate, the quantity of technological processes is less, the output is high, and the dendrobium officinale stem cell obtained is anti-aging and oxidation resistant, promotes hair growth and high baldness-resistant activity, and can be manufactured into functional health care products and cosmetics for preventing and curing various senescence and baldness and relevant Chinese drugs for preventing senescence and baldness.

Description

technical field [0001] The invention belongs to the field of medicinal plant stem cells, and in particular relates to stem cells of Dendrobium officinale Kimura et Migo and an isolation and culture method thereof. Background technique [0002] Plant stem cells, also known as meristems, are the source of plant growth and development and the center of signal regulation. Since plant stem cells are undifferentiated cells, they have strong self-renewal and continuous division capabilities, and are "immortal cells" with permanent viability; in addition, plant stem cells can also prevent genetic damage through self-apoptosis, avoiding future The defective DNA is passed on, thus protecting the plant from harsh environments. [0003] Studies have shown that plant stem cells are activators of human stem cells; plant stem cells have strong antioxidant and anti-aging activities, can significantly improve human immunity and anti-cancer capabilities, and open up a new way for human healt...

Claims

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Application Information

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IPC IPC(8): C12N5/04A61K36/8984A61K8/97A61P17/14A61P39/06A61Q19/08A61Q5/12
CPCA61K36/8984A61P17/14A61P39/06C12N5/04
Inventor 古焕庆
Owner STEMCELL ESSENTIALS
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