664 results about "Dendrobium candidum" patented technology

This invention relates to polysaccharides extract from Dendrobium candidum and its preparation method. The molecular weight of the polysaccharide extract of Dendrobium candidum is 10000-300000. The content of polysaccharide is no less than 25 wt%. The invention also relates to a Chinese medicinal composition containing polysaccharide extract from Dendrobium candidum and its preparation method, and application of the polysaccharide extract in preparing medicine for auxiliary treatment of diseases caused by hypoimmunity. The extract and Chinese medicinal composition containing the same can improve immunologic function with high bioavailability, no toxicity and adverse side effect. the preparation method is simple and convenient for operation and commercial process.

The invention discloses a tree tissue culture method of dendrobium candidum, which belongs to the agriculture technical field, and aims at solving the technical problem that a tree tissue culture method of the dendrobium candidum is provided, and belongs to the bionics wild cultivation. In order to solve the problems, the tissue culture method of the dendrobium candidum comprises the following steps that: 1) a tree with surface of a tree bark having grains is selected as an epiphytic tree, a dendrobium candidum seedling after being domesticated is selected as an epiphytic seedling, and a hay band is selected as a binding rope; 2) the epiphytic seedling is arranged on the surface of a trunk of the epiphytic tree, and root of the epiphytic seedling is wound by the binding rope to be fixed; and 3) the tree tissue culture humidity is controlled at 70 percent to 85 percent. Due to the adoption of the method, the dendrobium candidum can be planted on the tree with the surface having the grains.

The invention discloses a three-dimensional original ecological cultivation method for dendrobium candidum in forest. The three-dimensional original ecological cultivation method comprises the following steps of: firstly, selecting a cultivation environment; secondly, preparing a cultivation pot; thirdly, paving a matrix in the pot; fourthly, selecting seedlings; fifthly, permanently planting; sixthly, planting in a hanging manner or binding the seedling on the tree; seventhly, managing the cultivation; and eighthly, harvesting. The three-dimensional original ecological cultivation method disclosed by the invention provides a three-dimensional environment of original forest; the healthy growth of the dendrobium candidum is ensured; and the industrial and scale development of the dendrobium candidum can be realized; and the three-dimensional original ecological cultivation method disclosed by the invention has the advantages of high survival rate of seedlings, high yield, high quality of products, low cultivation cost and simpleness for management and operation.

The invention discloses a method for one-time separation and extraction of dendrobe polysaccharide, dendrobine and erianin from dendrobium candidum. The method for extraction of dendrobe polysaccharide, dendrobine and erianin from dendrobium candidum comprises the steps of crushing of dendrobe, enzymatic hydrolysis, ultrasonic extraction, microfiltration, ultrafiltration and nano-membrane concentration, solid-liquid separation, vacuum cryogenic distillation, solvent extraction, solvent recovery, recrystallization, vacuum low-temperature drying and purification in the carbon dioxide supercritical fluid, thereby obtaining the dendrobe polysaccharide, dendrobine and erianin. The prepared product has good color, good solubility and high active ingredients.

The invention discloses a method for cultivating algam Dendrobium nobile in group in water. Since the blades of algam Dendrobium nobile before output bottle will not form complete horny layer to process effective photosynthesis, according to the growth and physiological characters, the algam Dendrobium nobile output bottle is cultivated by water cultivate device, to be cultivated for 30 days at right condition, therefore, cultivated seeds can be transplanted into large field. The invention can effectively resolve the problems as low survival rate and improve survival rate to 95%.

The invention belongs to a planting method of plants, and particularly relates to a method for planting dendrobium candidum through imitating wild hanging trees. The adopted technical scheme is as follows: the method for planting dendrobium candidum through imitating wild hanging trees is characterized by comprising the following steps of: planting 3-5 cm dendrobium candidum large seedlings or domesticated seedlings in plastic flowerpots with a port diameter of 12-15 cm, hanging the flowerpot with dendrobium candidum planted on evergreen tree, applying routine nutrient solution and water on the dendrobium candidum large seedlings or domesticated seedlings, and spreading lime around the evergreen tree, wherein the bottom layer of the medium in the flowerpots is 50% of crushed wood blocks and the upper layer is sawdust mixture. By adopting the scheme, aiming at the defects of the prior art, the invention provides a novel method for planting dendrobium candidum which has a high growth rate and can be cultivated in a large scale through imitating wild hanging trees.

The invention discloses a processing method for dendrobium candidum health-card tea, which mainly comprises the following steps of: pre-processing, airing, drying, de-enzyming, rolling, initially drying, forming, drying and enhancing aroma. According to the processing method, reasonable development and utilization of tissue culture seedling during production of dendrobium candidum seedling is realized, the dendrobium candidum health-card tea has unique aroma which ordinary tea does not have, and can be eaten after drinking, a sticky feeling is generated by chewing, and moreover, the dendrobium candidum health-card tea is low in processing cost, and is simple to prepare, easy to preserve and much convenient to drink.

The invention discloses an artificial light cultivating method of dendrobium candidum, belonging to the technical field of medical plant cultivation. The method comprises the following steps: naturalized young plants are transplanted to a nutrition pot or a potted tray containing base material and placed in a three-dimensional cultivating shelf of an artificial light closed plant factory; wherein cultivating conditions are as follows: the illumination intensity is 60-150 mu mol.m-2.s-1; the illumination time is 9-12h.d-1, the temperature is 24-27 DEG C; the relative humidity is 65-80%; the CO2 concentration is 400-800 mu mol.m-1; and the air velocity is 0.3-0.6 m.s-1; after the young plants are transplanted, atomizing keeps the humidifying state of leaf surfaces of the young plants before 2 weeks; after two weeks, the young plants are watered; according to the principle of keeping the humidification of the base material, macroelement nutrient solution is applied every 7 to 15 days; microelement nutrient solution is applied every 25 to 35 days; bottom surface is watered every 3 to 5 days; the young plants are harvested after being cultivated for 1.5 to 2 years and every half year is a picking time. The method shortens the production period, guarantees the uniform quality of the products, increases the yield, and is easy to realize standardization, industrialization and scale production.

The invention relates to a cultivation method of dendrobium candidum, comprising the following steps of: paving the ground: levelling a land, and then sequentially paving a quicklime powder layer, a gardening ground cloth, a gravel layer and a shading net layer from bottom to top; forming rectangular land; building a shed; selecting the specifications of species and germchits: selecting seed sources in Zhejiang and domesticated seedlings obtained by domesticating tissue culture seedlings transplanted out of bottles for more than one year; and culturing. In the invention, the layers of multiple-layer ground pavements are not mutually influenced, the gravel layer can be used as a water filtering layer to provide a good root growth environment for the dendrobium candidum, and the multiple-layer ground pavements also physically obstruct insect pest sources from underground to a peripheral space with a matched insect prevention measures together, thereby not only saving the cost by reducing the use amount of pesticides, but also greatly enhancing the edible safety of the dendrobium candidum. The cultivation method of the dendrobium candidum creates an optimal environment for the growth of the dendrobium candidum from roots and the ground aiming at the artificial cultivation of a rare and endangered traditional Chinese medicinal material, i.e. the dendrobium candidum, and is used for carrying out pest control by adopting a full-physical method, thereby effectively reducing the generation of plant diseases and insect pests and realizing the safe and efficient cultivation.

The invention relates to an original ecological culture method of dendrobium candidum and belongs to the technical field of biological resource culture. The original ecological culture method of the dendrobium candidum is characterized in that the natural growth forest environment is selected, the sun shading effect of branches and leaves is utilized, the natural sun shading degree is 70 percent to 80 percent, the light irradiation is diffused light or scattered light, the light illumination intensity is 3000 to 5000lux, culture media are washed away from seedlings for field planting, the roots of the dendrobium candidum are placed onto culture substrates in a way that 3 to 5 strains are used as one bundle according to the bundle distance being 8-12*18-22cm, the roots are covered until the roots cannot be seen, sufficient water is watered, the air humidity is ensured to be 80 to 95 percent, the temperature is 19 to 33 DEG C, the water spraying is carried out for 1 to 2 times every day after the planting, and the water spraying is sufficient in each time. The original ecological culture method of the dendrobium candidum has the advantages that the natural growth forest environment is used as a carrier, high-quality and efficient culture substrates are adopted, the environment favorable for the growth of the dendrobium candidum is formed, the survival rate of the dendrobium candidum is as high as more than 95 percent, in addition, the yield is high, and the culture cost is low.

The invention provides a Dendrobium candidum polysaccharide buccal tablet comprising the following components by weight portions: 30-100 portions of Dendrobium candidum polysaccharide, 380-420 portions of xylithol as a carrier, 300-340 portions of aspartame, 0.5-1.5 portions of menthol, 0.5-1.5 portions of aerosol and 4-6 portions of magnesium stearate; and the invention also provides a preparingmethod of the Dendrobium candidum polysaccharide buccal tablet, comprising the following steps: taking the Dendrobium candidum polysaccharide as raw material, adding auxiliary material, uniformly mixing according a certain proportion, and tabletting and shaping to obtain the product. The Dendrobium candidum polysaccharide is easy to be taken, and fresh and cool in mouthfeel, can be directly absorbed through oral mucosa, avoids loss generated when nutrients are absorbed by organs such as human gastrointestine, liver and the like, improves the absorption utilization ratio of nutrients in Dendrobium candidum polysaccharide, and has important significance to clinical treatment and the prevention and development of health care products.

The invention provides dendrobe health-care tea and a manufacturing method. The dendrobe health-care tea is prepared by taking Yunnan big-leaf tea fresh leaves and medical dendrobe as raw materials according to a conventional tea making process. Dry tea raw materials are prepared by selecting one-bud, one-bud and two-leaves or one-bud and three-leaves Yunnan big-leaf tea fresh leaves according to the a conventional tea making process. The dendrobe raw material is selected from any one of purple dendrobe, dendrobium candidum and Dendrobium aphyllum. The dendrobe health-care tea keeps the appearance of the tea, obviously reduces the bitter and astringent tastes, embellishes mouths and throats, is enriched with dendrobe effective components and has a better health-care effect; and the manufacturing method is simple and feasible.

The invention discloses a processing method of tea making with dendrobium candidum leaves. The method comprises the steps of fresh leaf treatment, high-temperature withering, rolling, fermentation, drying and deodorizing, wherein in the high-temperature withering step, the treated fresh dendrobium candidum leaves are uniformly placed on a withering bed and continuously turned over for 3-5 hours at the constant temperature of 70-90 DEG C to allow the water content of the leaves to reach 60-70%, and the leaves are put away if no crackly fracture sound is heard when the leaves are caught up by hand and pinched forcibly. The method solves the problems that the existing dendrobium candidum leaves are wasted, tea prepared by the dendrobium candidum leaves is inconvenient to drink, and effective components are difficult to utilize; and the method has the advantages of being capable of making full use of a rare resource of dendrobium candidum, effectively increasing the leaching rate of medicinal components of dendrobium candidum, and facilitating brewing.

The invention discloses a method for processing dendrobium candidum gynostemma pentaphyllum zhulan scented tea. The method comprises the steps of: A. picking fresh and tender stem tips of gynostemma pentaphyllum to be stacked, steaming and removing green by using a stainless steel tray with meshes; B. fast pushing the fresh and tender stem tips of gynostemma pentaphyllum to be spread for cooling,conducting convection blowing to the downside of a fan, and baking; intermittently baking and dewatering by using a baking bed with a moisture extraction hood, spreading before baking, taking out forcooling, drying, cooling, and spraying a dendrobium candidum extracting solution sieved with a 120 thousand-mesh molecule sieve, wherein the concentration of the dendrobium candidum extracting solution is 10%; and C. baking to be dry: baking by using a famous tea baking bed with a moisture extraction hood, taking out for cooling, baking for increasing scent, adsorbing Chloranthus flower essentialoil with absorbent wool according to 30 equal parts of tea leaves, then placing in a breathable box, filling the dendrobium candidum gynostemma pentaphyllum tea into a ceramic pot, separately placingscented boxes into a pot bottom layer, a pot middle layer and a pot upper layer, and smoking to obtain dendrobium candidum gynostemma pentaphyllum zhulan scented tea. The method is easy, simple and convenient to operate, and sweet in taste, has no obvious bitter taste, is non-irritating to intestines and stomach, and has the obvious effects of relaxing the bowels, invigorating stomach and decreasing blood sugar.

Disclosed is a pharmaceutical composiiton containing dense dendrobium and preparation process, wherein the composition comprises (by weight ratio) dense dendrobium juice with honey 10-70%, Siberian solomonseal rhizome 30-90%. The composition may also contain gen-seng and lucid ganoderma to be made into oral liquid.

The invention relates to a dendrobium candidum chewable tablet. The chewable tablet consists of dendrobium candidum fine powder and medicinal acceptable auxiliary materials, and is characterized in that: the particle diameter of the dendrobium candidum fine powder is 34-58 mum. The dendrobium candidum chewable tablet disclosed by the invention has high polysaccharide dissolution rate, and contributes to intestinal absorption of active ingredients; and the bioavailability of a preparation can be improved remarkably.

The invention discloses a manufacturing method of dendrobium candidum wallex lindll pure powder tablets. The method comprises steps that: (1) fresh dendrobium candidum wallex lindll stem segments are washed, and dripped; (2) the dripped dendrobium candidum wallex lindll is placed in lyophilizing equipment, and is pre-lyophilized for 1 to 3 hours under a temperature of -30 DEG C; the temperature is then reduced to -35 DEG C, and the temperature is maintained for 2 to 4 hours; (3) the lyophilized dendrobium candidum wallex lindll is processed from gelsiccation; the dendrobium candidum wallex lindll is vacuumed, and is dried by heating by using a heating plate in the equipment, wherein 35 DEG C consistent-temperature drying is carried out for 6 to 9 hours, and then 45 DEG C consistent-temperature drying is carried out for 6 to 8 hours; (4) dried dendrobium candidum wallex lindll is crushed by using a crusher, such that powder is obtained; (5) ultra-fine crushing is carried out upon the dendrobium candidum wallex lindll powder by using supersonic waves, dendrobium candidum wallex lindll cell wall-broken ultra-fine powder is obtained; (6) the obtained ultra-fine powder is processed from cobalt 60 radiation sterilization, and is pressed into tablets by using a tablet machine, such that dendrobium candidum wallex lindll pure powder tablet are obtained. With the method, dendrobium candidum wallex lindll is processed into ultra-fine powder, and nutrients are easy to dissolute, such that the tablets are easy to be absorbed by human bodies.

The invention discloses a method for efficiently and rapidly propagating Dendrobium candidum test-tube seedlings in a large scale. The method provided by the invention comprises the following steps of: disinfecting Dendrobium candidum capsules, rinsing and slicing off to obtain seeds; sowing seeds into a liquid culture medium and carrying out a shaking culture for 50-60 days; germinating seeds to form protocorms and further forming gemmules; putting the gemmules into a strong seedling culture medium to be cultured for 2-2.5 months to form plantlets with 2-3 leaves and 1-2 radicles; transferring the radicles into a strong seedling rooting culture medium and covering an opening sealing membrane on a bottle opening of a tissue bottle; transferring the tissue bottle to a plastic greenhouse seedling bed with an outer shade; providing sunlight or natural light and culturing for 2-3 months to obtain bigger seedlings; taking out the bigger seedlings from the bottle, washing, disinfecting and airing; then transplanting the bigger seedlings into cultivation matrix of a plastic greenhouse to be cultured for 24-30 months to obtain the product. The process provided by the invention can be directly used for industrially producing germchit in a large scale and has the advantages of less pollution, high seed germination rate, low variation rate, strong germchit, low production cost, high transplanting survival rate and the like.

A dendrobium candidum planting method specifically includes the steps that dendrobium candidum seedlings are planted on tree species with longitudinal grooves in the surface of bark; before planting, the roots of the dendrobium candidum seedlings dip in a solid nutrient solution prepared through a high-water-absorbent material and a Jin Weiguo effective foliar fertilizer produced by the America Grow More Inc; during planting, the dendrobium candidum seedlings are bound on trunks through binding strips manufactured through shading nets so that the dendrobium candidum seedlings can be attached to the trunks. According to the method, the trunks serve as epiphytes, tress are used for shading, plastic greenhouses and other infrastructure do not need to be built, substrates are not used, cost is reduced by nearly a half, and environment is not influenced. Meanwhile, dendrobium candidum products cultivated through live trees are more ecological, higher in polysaccharide content and higher in quality. Dendrobium candidum is planted on the trees, does not occupy fertile farmland suitable for grains and does not occupy forest land suitable for the trees, and comprehensive benefits of the forest land can be better achieved. Most importantly, the live trees are planted in the wild, restoration of wild populations is facilitated, and sustainable development is achieved.

The invention discloses an industrial cultivation method for dendrobium candidum, belonging to the technical field of Chinese herbal medicine cultivation. The industrial cultivation method disclosed by the invention comprises the following steps of: taking freshly wild dendrobium candidum seeds as a raw material, thereby obtaining a dendrobium candidum sterile cultivating system; the industrial cultivation method comprises related technologies, such as seed treatment, seedling propagation, seedling tissue-cultivation and seedling training, transplantation and the like, so that the source of the dendrobium candidum seedlings and the geo-authentic character of the seedlings are effectively ensured; compared with the conventional seedling propagation, the seedling propagation speed is increased by 37%, the seedling training and transplantation survival rates are increased to above 90%, and the yield is increased by above 30%; and compared with the active ingredient content of wild dendrobium candidum, the dendrobium candidum, cultivated according to the method disclosed by the invention, comprises a same polysaccharide content as the wild dendrobium candidum, and slightly higher amino acid content as well as trace-element content than the wild dendrobium candidum.

The invention relates to a traditional Chinese preparation, and particularly relates to traditional Chinese medicine dendrobium candidum buccal tablets. 1000 traditional Chinese medicine dendrobium candidum buccal tablets are prepared from the following raw materials according to a weight ratio: 100 g of dendrobium candidum, 1-500 g of filling agent, 1-40 g of adhesive, 1-10 g of glidant and 0.1-2 g of lubricating agent.

The invention discloses a rapid tree cultivation method for dendrobium candidum tissue-culture bottle seedlings. The rapid tree cultivation method is to bind dendrobium candidum tissue-culture bottle seedlings after subjected to short-term refining on threes for cultivation, and includes seedling hardening, seedling fetching, pretreatment of seedlings, treatment on tree bark and moss, preparation of a binding material, binding of the seedlings, spraying sanitization, spraying management and final-period management. According to the invention, the tree cultivation method is simplified, seedling hardening and tree cultivation effects are optimized, production period is shortened, relatively high seedling survival rate is guaranteed as the survival rate is above 0.095, the obtained medicine is high in quality, multiple effects of speediness, high yield and excellent production are achieved, and the rapid tree cultivation method for dendrobium candidum tissue-culture bottle seedlings is an efficient and feasible tree cultivation method for dendrobium candidum.

The invention relates to a preparation method of small molecular weight dendrobium candidum polysaccharides. The preparation method comprises following steps: A, bacterial strain activation and expanding culture are carried out, wherein under aseptic conditions, an appropriate amount of a liquid culture medium is used for preparing a fusarium avenaceum bacterium suspension; B, high pressure homogenization is repeated for 5 to 10 times using a high pressure micro jet homogenizer so as to obtain a dendrobium candidum wall-broken homogenate; C, fermentation culturing is carried out, wherein an appropriate amount of a liquid culture medium is injected into a fermentation tank, the dendrobium candidum wall-broken homogenate and the fusarium avenaceum bacterium suspension are weighed, and are delivered into the fermentation tank for fermentation so as to obtain a fermentation liquid; and D, polysaccharide purification is carried out, wherein after fermentation, active carbon is used for decolouring, an obtained product is allowed to stand for 1h for absorption, filtering is carried out so as to remove residue and obtain a filtrate, vacuum pumping filtration is carried out so as to obtain a precipitate, the precipitate is washed with ethanol for three times, and vacuum drying is carried out so as to obtain the small molecular weight dendrobium candidum polysaccharides. The average molecular weight of the small molecular weight dendrobium candidum polysaccharides is lower than 300kDa; and the small molecular weight dendrobium candidum polysaccharides are capable of promoting propagation of skin probiotics, increasing the density of langerhans cells on skin, and improving skin immunity.

The invention relates to a seed tissue culture propagation method of dendrobium candidum and belongs to the technical field of planting of traditional Chinese medicinal materials. The seed tissue culture propagation method of dendrobium candidum comprises the following four steps of: introduction and sterilization, induction culture, differentiation culture as well as strong seedling and rooting culture. The seed tissue culture propagation method of dendrobium candidum has the beneficial effects that seedlings obtained by seed tissue culture are difficult to degenerate, genetic traits are stable, active substance content is close to that of a wild variety, and quality of seeds is guaranteed; quantity of the seeds of dendrobium candidum is high, and propagation quantity of the seeds can be greatly increased; the seed tissue culture propagation method of dendrobium candidum also greatly shortens a tissue culture propagation period of dendrobium candidum, the tissue culture period from seed shearing to rooting and seedling growing is only 4-5 months, production time is greatly shortened, and production cost is reduced.

The invention relates to a compound dendrobium candidum buccal tablet and a preparation method of the compound dendrobium candidum buccal tablet and aims at reaching the effects that the absorption and utilization efficiency and speed of the dendrobium candidum can be respectively improved and accelerated through the provided buccal tablet, and the provided preparation method has the characteristics that the process is simple, and the operation is convenient. The compound dendrobium candidum buccal tablet and the preparation method have the technical scheme that the compound dendrobium candidum buccal tablet comprises compound dendrobium candidum extract extractum, mannitol, dextrin and magnesium stearate which are mixed according to the weight proportion, and the compound dendrobium candidum extract extractum comprises dendrobium candidum, American ginseng, Chinese wolfberry fruit, rhizoma phragmitis, okra, mulberry and astragalus extracts which are determined according to the weight proportion. The preparation method of the buccal tablet comprises the following manufacture steps that (1) the materials are taken according to the raw material proportion; (2) the American ginseng is subjected to backflow extraction processing to obtain extractum A; (3) the extracted American ginseng medicine slag is mixed with other raw materials, decoction and extraction are carried out, and extractum B is obtained; (4) the extractum A and the extractum B are mixed and are then concentrated, and the compound dendrobium candidum extract extractum is obtained; (5) granulation is carried out; and (6) tabletting is carried out.

The invention discloses a method for planting dendrobium candidum in a wild imitating understory condition. The method comprises: selecting a wild imitating forest land; preparing soil and staking out; preparing sprouts; binding trees and planting; and managing the sprouts after planting. The method specifically comprises the steps: binding dendrobium candidum on artificial stubs by using a non-woven fabric as a bandage; and then putting the artificial stubs in an understory manner for natural growth. A light source required by growth of dendrobium candidum and the area of the forest land are effectively utilized for being combined with normalized culture management. As dendrobium candidum is planted by virtue of the method, dendrobium candidum is few in disease, is basically free of chemical fertilizers and pesticides and is relatively excellent in quality. The method has the advantages of being simple in operation, easy to master, strong in normalization, energy-saving, environmental-friendly and pollution-free and the like.