33 results about "Dendrobium candidum" patented technology

The invention belongs to the technical field of plant protection, and particularly relates to a Tulasnella calospora fungus strain SH1 and application of the Tulasnella calospora fungus strain SH1 in promoting germination of dendrobium chrysanthum seeds. The preservation number of the fungus strain SH1 is GDMCC No: 66867. The fungus strain SH1 and mature dendrobium chrysanthum seeds are subjected to symbiotic germination, and the germination rate of the seeds can be remarkably increased. Results of the embodiment show that when the fungus strain SH1 is used for symbiotic germination of dendrobium chrysanthum seeds, the germination rate can reach 73.20% at most.

The invention discloses an application of Dendrobium officinale-derived exosome-like nano-vesicles in stimulation of polarization of macrophages into M2-type macrophages, and finds that when the Dendrobium officinale-derived exosome-like nano-vesicles are used for stimulation of the macrophages, the macrophages can be effectively polarized and converted into the M2-type macrophages.

The invention belongs to the technical field of processing technologies of dendrobium candidum tea, and particularly relates to dendrobium candidum composite tea as well as a preparation method and application thereof. The dendrobium officinale composite tea is prepared by mixing dendrobium officinale stems, dendrobium officinale leaves, Chinese wolfberry fruits, roses and licorice roots according to the proportion of (2.4 g-4g): (0.4 g-0. 8g): (2.535 g): (0.688 g: 0.400 g). The dendrobium officinale stems and the dendrobium officinale leaves are obtained through vacuum freeze drying. The dendrobium officinale composite tea is high in leaching rate and can effectively resist oxidation and maintain the healthy level of blood sugar.

The invention relates to a method for preparing high-solubility dendrobium officinale powder based on a polysaccharide enzymolysis technology, and belongs to the technical field of food processing. The invention relates to a method for preparing high-solubility dendrobium officinale powder based on a polysaccharide enzymolysis technology, which comprises the following steps of: crushing, grinding and sieving dendrobium officinale dry strips to obtain dendrobium officinale dry powder, and adding the dendrobium officinale dry powder into purified water to obtain dendrobium officinale dispersion liquid; heating the dispersion liquid in a water bath at 90-100 DEG C for 1-2 hours, centrifuging, and collecting supernate to obtain an extracting solution; and adding glycosidase into the extracting solution, carrying out enzymolysis at 40-60 DEG C for 1-1.5 hours, and carrying out enzyme deactivation, high-pressure homogenization and spray drying to obtain the high-solubility dendrobium officinale powder. By means of enzymolysis treatment, the yield of the effective component (polysaccharide) in the dendrobium nobile powder is increased. The method is easy to operate, mild in condition and relatively short in production period, other substances cannot be introduced, and adverse effects on the flavor of the prepared high-solubility dendrobium officinale powder cannot be caused.

The invention discloses a sterile production method of a fresh dendrobium officinale protocorm product, which comprises the following steps: taking dendrobium officinale seeds from capsules of dendrobium officinale, repeatedly washing the capsules with tap water until the capsules are free of foam, sterilizing, cutting the capsules open, and drying to obtain the dendrobium officinale protocorm product. Uniformly spreading the seeds on an M2 solid culture medium, keeping the illumination condition of 500Lx and the temperature of 25 + / -1 DEG C, and waiting for the seedlings to grow for 60-90 days; wherein the M2 solid culture medium comprises 1 / 2 MS macroelements without exogenous hormones, 35% of banana juice, 35% of composite carbon source and 2% of agar powder. The method has the beneficial effects that a technical route map for mass production by infinitely stacking sterilization and ventilation culture of the small glass containers of 10 liters and getting rid of the constraint of a shaking table is provided. The technical key point is to screen out that the growth rate which can reach 8.4 mg / g.d without depending on plant growth hormone (which is a rigid biological index).

The utility model discloses a kind of Dendrobium candidum transplanting planting devices, it is related to Dendrobium planting technical field.The utility model discloses a kind of Dendrobium candidum transplanting planting devices, it is related to Dendrobium planting technical field.The utility model discloses a kind of Dendrobium candidum transplanting planting devices, it is related to Dendrobium planting technical field.

The invention discloses a novel application of VCH-916 in increasing the content of dendrobine in dendrobium nobile lindl. Belongs to the field of plant biotechnology. By applying an effective dose of VCH-916 to dendrobium nobile, a dendrobine biosynthesis pathway of dendrobium nobile can be remarkably activated, so that the accumulation amount of dendrobine is greatly increased. The method is easy and convenient to operate, remarkable in effect and high in safety, and has great significance in solving the problem of shortage of high-quality dendrobium nobile resources and improving the medicinal material value and economic benefits of the dendrobium nobile.

The present application relates to a preparation method and application of Dendrobium candidum extract, and belongs to the technical field of plant extraction. The present application realizes physical disruption of cell structure by using ultra-low temperature crushing technology, and avoids degradation of heat-sensitive components. A complex system of cellulase, pectinase and neutral protease is used for synergistic enzymolysis at 35-45 DEG C and pH 5-6, which efficiently hydrolyzes cell wall components and releases bound anti-inflammatory active ingredients. Subsequently, Ruminococcus lactaris is used for anaerobic fermentation, and the beta-glucosidase secreted by the bacteria hydrolyzes the bibenzyl aglycone, the metabolic product lactic acid promotes the protonation of alkaloids to form water-soluble salts, and the exopolysaccharide forms a complex active ingredient with the dendrobium polysaccharide; the fermentation broth is filtered, and is compounded with the ultrafiltration purified water extract obtained by ultrasonic assisted extraction at a ratio of (2-4):1, and finally high-purity extract is obtained by freeze-drying. The extract can be released controllably in the gastrointestinal tract, and the inhibition rate of LPS-induced inflammation is better than that of traditional processes, and can be used in the field of intestinal anti-inflammatory.

The utility model belongs to the technical field of disinfection devices, and particularly relates to a disinfection device for planting dendrobium devonianum, which comprises a barrel body, a pesticide spraying device is arranged on the barrel body, a cover is slidably connected to the upper end of the inner side of the barrel body, a motor is fixedly mounted at the upper end of the cover, and the output end of the motor is rotatably connected with the cover. And the output end of the motor is fixedly connected with a mounting seat, the outer side of the mounting seat is fixedly connected with a sleeve, and the interior of the sleeve is slidably connected with a sliding rod. According to the disinfection device for planting dendrobium devonianum, the motor, the mounting seat, the scraper blade and other parts are arranged, so that pesticide and water in the barrel body can be fully mixed, and the spring is arranged to drive the scraper blade to be attached to the inner wall of the barrel body, so that the pesticide is prevented from remaining on the inner wall of the barrel body, and the problems that when the disinfection device for planting dendrobium devonianum is used, the pesticide and water are not uniformly mixed, and the use is inconvenient are solved. And the concentration of the sprayed liquid medicine is too high or too low.

The present application relates to Dendrobium officinale flower extract, in particular to a Dendrobium officinale flower active part in the preparation of a drug for treating cervicitis; a Dendrobium officinale flower active part in the preparation of a drug for treating cervicitis; characterized in that the Dendrobium officinale flower active part is prepared by the following steps: weighing 4g of Dendrobium officinale flower, adding 200mL of purified water, boiling water bath for 1h, extracting 3 times, then filtering and combining the filtrate, removing most of the water, and then water bath evaporation; the extract is added with 100mL of purified water to prepare a suspension, extracted with ethyl acetate, and extracted 3 to 4 times until complete extraction; the concentrated extract is the ethyl acetate part DOF- EA of the Dendrobium officinale flower boiling water extract. The present application adopts a mouse cervicitis model induced by phenol glue, and DOF- EA is administered by gavage, and the results show that DOF- EA can significantly improve the damage of cervical mucosa tissue, reduce the level of inflammatory factors and oxidative stress, and has a good effect on anti-cervicitis.

The application discloses a kind of based on medicine and food homology dendrobium compound product and preparation method thereof, belong to food technical field, wherein, a kind of based on medicine and food homology dendrobium compound product, including following weight parts raw materials Effective component and pharmaceutically acceptable adjuvant: dendrobium 20-40 parts, medlar 15-25 parts, American ginseng 25-35 parts, ganoderma 15-25 parts, D-mannitol 10-20 parts.The application will dendrobium, American ginseng, ganoderma, medlar four medicinal materials scientific compatibility, wherein dendrobium nourishing yin is monarch, American ginseng yin and qi double supplementation is minister, ganoderma is right and solid foundation, medlar nourishing liver and kidney is make, follow the theory of "monarch, minister, right and solid foundation, make" of traditional Chinese medicine.Four drugs are used together, and the effect of common yin, enhancing immunity, relieving fatigue is played, and the common problem of modern people's complex sub-health is solved, and the effect is more comprehensive and balanced than single formula product.

The present application belongs to the field of fingerprint, and particularly relates to a method for detecting medicinal stems of Dendrobium candidum from Sichuan origin. The specific technical scheme is as follows: a method for constructing a Dendrobium fingerprint, comprising the following steps: mixing a Dendrobium powder to be tested with methanol, performing ultrasonic treatment, taking out, cooling, and filtering; evaporating the filtrate under reduced pressure, dissolving the residue with methanol, filtering, shaking, and obtaining a test solution; taking the test solution, performing high performance liquid chromatography detection, and recording a fingerprint; and the chromatography detection has the following chromatography conditions: a C 18 reverse-phase chromatography column; a column temperature of 30 DEG C; a detection wavelength of 270 nm; and a total flow rate of 0.8 mL / min. The method provided by the present application is simple to operate, has complete characteristic components, and has a stable test solution, high precision, and good reproducibility.

The invention belongs to the technical field of plant puree preparation, and particularly relates to dendrobium puree as well as a preparation method and application thereof. According to the preparation method, firstly, a 1% sodium bicarbonate aqueous solution is used for conducting heat extraction on dendrobium nobile, the 1% sodium bicarbonate aqueous solution can neutralize the acid environment, and acidic hydrolysis of polysaccharide in the extraction process is inhibited; according to the method, an ethanol solution with the concentration of 50% is added into dendrobium residues extracted by a sodium bicarbonate aqueous solution with the concentration of 1% for re-extraction, gradient separation of components can be realized by using solvent systems with different polarities, and the ethanol solution with the concentration of 50% can dissolve acidic polysaccharide which is not dissolved by alkaline water in dendrobium and can effectively extract medium polar substances such as flavones and glycosides at the same time. In addition, a water-saturated n-butyl alcohol solution is continuously added into the extracted dendrobium residues for extraction, and residual polysaccharide and saponin components in the dendrobium are efficiently extracted. Through the steps, the raw stock components in the dendrobium nobile can be effectively reserved, and the dendrobium nobile has an excellent anti-inflammatory effect.

The application discloses a method for inducing somatic embryogenesis by using dendrobium candidum stem segments, which comprises the following steps: taking the dendrobium candidum stem segments with dormant buds as explants; disinfecting the explants; uniformly scratching the surface of the disinfected explants; performing skinning treatment on the disinfected explants; performing dormant bud development induction culture on the skinned explants to obtain the dendrobium candidum stem segments with clustered buds; cutting the dendrobium candidum stem segments with clustered buds to remove the bud tips; uniformly scratching the cut surfaces; and performing somatic embryogenesis induction culture to obtain the dendrobium candidum somatic embryos. The method can be used for directly inducing the dendrobium candidum somatic embryos on a large scale by using the dendrobium candidum stem segments, and the induction time is short. The new buds can be used for successfully inducing the somatic embryo clusters in 60-90 days, so that the dendrobium candidum can be rapidly expanded and multiplied. The method is simple in operation, low in cost, and can be used for inducing the dendrobium candidum without seeds. The method is genetically stable, and can be used for large-scale proliferation in a short time according to the requirements.

The utility model relates to a dendrobium planting equipment technical field especially relates to a kind of sunshade devices for dendrobium candidum planting, including fixed frame, planting groove, rack and sunshade mechanism, fixed frame is rectangular frame body structure, and the upper portion of fixed frame is provided with planting groove, and the upper portion of planting groove is provided with sunshade mechanism;The sunshade mechanism includes first fixed support, first rotating roller, transmission chain, receiving frame, second transmission sprocket and transmission gear, first fixed support is movably connected with the slide strip on fixed frame by the sliding slot of both sides, and the inside of first fixed support is provided with first rotating roller, and first rotating roller is connected with the second transmission sprocket in receiving frame by transmission chain by the first shaft of both sides through;Second transmission sprocket is set on second shaft, and transmission gear is set on second shaft.The utility model is used to solve the problem, such as inconvenient operation, low efficiency of existing sunshade mode, for the growth of dendrobium candidum provides more suitable, stable illumination environment.

The invention relates to application of dendrobium candidum polysaccharide as a largemouth bass feed additive, the dendrobium candidum polysaccharide is added into largemouth bass feed, and the content of the dendrobium candidum polysaccharide is less than or equal to 2.5 g in every 1 kg of feed. Dendrobium officinale is added into the feed, so that growth can be promoted, liver and intestine health can be regulated, and the survival rate can be increased.

The invention belongs to the technical field of candies, and particularly relates to a dendrobium coffee component candy and a processing method thereof, the dendrobium coffee component candy comprises the following components: 40-50% of white granulated sugar, 17.5-27.5% of glucose syrup, 20.5-24.5% of clear water, 2.5-6.5% of dendrobium powder, 3.8-4.2% of black coffee powder, 0.3-0.8% of citric acid, 0.1-0.5% of vanillin, and 0.5-0.9% of butter. The dendrobium nobile powder is introduced into a candy system and is in synergistic compatibility with the black coffee powder, so that the good taste of the candy is maintained, the product is endowed with herbaceous plant flavor and certain nutritional attributes, and the functional application field of traditional candies is expanded.

The application discloses a Dendrobium candidum multi-index nondestructive quality evaluation method, and belongs to the technical field of traditional Chinese medicinal material nondestructive detection, and particularly relates to a Dendrobium candidum multi-index nondestructive quality evaluation method. The sample is clamped in a measuring cavity, an effective measuring area between nodes is determined, and zero-perturbation pre-scanning data are obtained, based on which a micro-perturbation script is generated, near-infrared pulse irradiation, air heat exchange perturbation and mechanical light tapping perturbation are applied under a unified time axis, diffuse reflection near-infrared spectrum, a thermal recovery curve, a vibration attenuation waveform and an epidermal texture change sequence are synchronously collected, a moisture-polymer coupling state tensor is constructed, and a quality evaluation result or an abnormal label is output.

1. The name of the design product: packing bottle (Dendrobium candidum original syrup). 2. The use of the design product: for Dendrobium candidum original syrup packing. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best indicates the design points: perspective view.

The invention relates to the technical field of dendrobium granule preparation, and discloses a dendrobium officinale composite granule preparation preparation device which comprises a machine body, a vibration assembly and a drying disc are installed in the machine body, the vibration assembly is connected with the drying disc, a discharging assembly is installed over the drying disc, and the discharging assembly is fixedly connected to the top of the machine body; the discharging assembly comprises a conveying pipe, a center shaft and a spiral conveying blade, a round hole is formed in the top of the machine body, the top end of the conveying pipe is fixedly connected with the inner wall of the round hole, the center shaft is driven to rotate in the conveying pipe, the spiral conveying blade is fixedly connected to the circumferential side face of the center shaft, and the outer side face of the spiral conveying blade is in abutting connection with the inner wall of the conveying pipe. The spiral conveying blade forcibly rotates to feed materials, traditional valve opening and closing type feeding is replaced, and the problem of extrusion and adhesion caused by the high-viscosity characteristic of dendrobium officinale wet particles due to rich polysaccharide is thoroughly solved.

The application provides a Dendrobium candidum exosome composition loaded with miR-146a and miR-124 and a preparation method and application thereof. The Dendrobium candidum exosome has an antioxidant function, miR-146a and miR-124 have a synergistic anti-inflammatory function, the Dendrobium candidum exosome provides a delivery carrier for miR-146a and miR-124 and improves the stability and anti-inflammatory function of miR-146a and miR-124, and finally a pharmaceutical composition EXE-miR-146a-124 for treating neuroinflammation and diseases related thereto is developed. The application not only provides a safe and efficient miRNA delivery system, but also endows the system with neuroprotective and anti-inflammatory effects, and provides a brand-new, natural source intervention scheme for the treatment of nervous system inflammatory diseases.

The invention belongs to the technical field of plant extracts and biological medicines, and particularly relates to a dendrobium nobile composition, a dendrobium nobile liquid preparation and application of the dendrobium nobile composition and the dendrobium nobile liquid preparation in the aspect of reducing uric acid. The dendrobium composition is prepared by compounding dendrobium officinale primary pulp and a coffee extract rich in chlorogenic acid according to a specific proportion, and is rich in coffee chlorogenic acid and dendrobium officinale polysaccharide. The coffee chlorogenic acid can reduce purine absorption, and chelated sodium ions inhibit urate crystallization; the dendrobium officinale polysaccharide can enhance the permeability of blood capillaries and increase the excretion function of kidneys, the dendrobium officinale polysaccharide and the kidney are compounded to form a dual acid reduction mechanism, and the uric acid reduction effect is remarkably superior to that of dendrobium officinale puree or coffee extract rich in chlorogenic acid with the same dosage. The herba dendrobii composition is mixed with a sour flavoring agent and a sweetening agent to form a plant flavor liquid preparation which is sour, sweet and palatable, no preservative or artificial color is added, the safety is higher, and the herba dendrobii composition is suitable for daily drinking of people with hyperuricemia and has the advantages of nutrition intervention and taste optimization.

The application of the Dendrobium candidum stem / leaf polysaccharide in preparing medicines or health foods for preventing and treating ulcerative colitis. The Dendrobium candidum stem / leaf polysaccharide obtained by extracting stems or leaves of specific Dendrobium candidum species by a specific extraction process has a higher content and a better prevention and treatment effect on ulcerative colitis, and can significantly alleviate the phenomenon of sudden weight loss of mice, reduce the DAI value of the colon tissue of ulcerative colitis mice, alleviate the abnormal atrophy or hypertrophy of the immune organs of mice, and restore the colon length. The Dendrobium candidum stem / leaf polysaccharide obtained by the application has the characteristics of definite curative effect, and has a good market development and application prospect.

The utility model provides a kind of bundling device for the trunk planting dendrobium candidum, it is related to dendrobium planting technical field, including fixed band, the outside of fixed band is provided with quick binding assembly, the quick binding assembly includes the fixed connector of being installed in fixed band one end, the other end of fixed band is equipped with movable connector, the inside of fixed connector is provided with antiskid clamping plate, the inside of movable connector is equipped with antiskid clamping nail;The utility model is equipped with quick binding assembly, when bundling, fixed clamping cone is inserted into trunk, fixed connector is fixed, so that fixed band is stretched around trunk and is fixed, ensure the convenience and effect when bundling, when movable connector and fixed connector are mutually buckled, antiskid clamping plate will extrude fixed band to recess on antiskid clamping plate, so as to avoid that fixed band appears sliding after bundling, ensure the stability of dendrobium candidum bundling fixation.

The present application relates to the technical field of Dendrobium candidum tea preparation, in particular to a Dendrobium candidum tea preparation equipment and production process, which comprises a base, a conical crushing bin is formed in the inside of the base, a crushing cone is arranged in the inside of the conical crushing bin, a sleeve rod is fixedly connected to the bottom of the crushing cone, an inner rod is movably inserted into the inside of the sleeve rod, an equipment bin is formed in the bottom of the base, an arcuate frame is fixedly connected in the inside of the equipment bin, a hollow wheel one and a hollow wheel two are rotatably connected to one end of the arcuate frame respectively, and a plurality of adjusting sliding grooves are formed in the outer periphery of the hollow wheel one and the hollow wheel two. The present application can simultaneously increase the rotating speed and reduce the gap for the annual old stems, and can simultaneously reduce the rotating speed and increase the gap for the annual tender stems, so as to avoid the exudation of mucilage and the adhesion of fine powder, thereby effectively improving the adaptability of the equipment to the Dendrobium candidum stems of different growth periods and ensuring the uniformity of the crushing granularity.

The invention discloses a tissue culture and rapid propagation method taking a dendrobium devonianum paxt stem segment as a starting material, and belongs to the technical field of plant tissue culture. The method comprises the following steps: after branches are collected in winter and dried in the shade, regularly spraying a hydrogen peroxide silver ion disinfectant with a specific concentration to induce axillary buds to germinate; taking germinated axillary buds as explants, disinfecting, and inoculating to a pre-culture medium; if pollution occurs, soaking with an original disinfectant and transferring into a rescue culture medium containing potassium sorbate for rescue; inoculating the sterile material to a starting culture medium, and performing one-step induction to form multiple shoots with roots; and finally, strong tissue culture seedlings are obtained through seedling strengthening and rooting culture. By means of unique explant pretreatment and induction, an efficient pollution rescue mechanism and an optimized culture medium system, stable and efficient rapid propagation of excellent characters of dendrobium devonianum is achieved, the propagation period is greatly shortened, and reliable technical support is provided for industrialization.

The invention relates to a plant endophyte as well as a preparation process and application of a fermentation product of the plant endophyte, and in particular, the plant endophyte is dendrobium officinale endophyte candida Gandida sp. IGLS2, and the preservation number is CCTCC (China Center For Type Culture Collection) M20242143. The endophyte can be fermented to obtain dendrobium officinale fermentation liquor without an additional carbon source, dendrobium polysaccharides can be effectively degraded, biotransformation is achieved, in addition, compared with an unfermented dendrobium officinale extracting solution, the capacity of inhibiting ROS and IL-6 is effectively improved, the cell migration rate is remarkably improved, and the cell activity is not affected under high concentration.

The application discloses a Dendrobium candidum composite extract with anti-lung cancer activity and a preparation method thereof, wherein the mass ratio of the Dendrobium candidum binzyl extract and the Dendrobium candidum polysaccharide is 1:1-1:2. The composite extract prepared by the application can inhibit the growth of Lewis lung cancer, and the effect is better than that of the binzyl or polysaccharide alone, and the composite extract plays a better synergistic effect, and is expected to be prepared into an anti-lung cancer drug. The application also establishes a detection method of the Dendrobium candidum composite extract, and the quality of the composite extract can be well detected.

The utility model relates to the technical field of dendrobium officinale planting equipment, in particular to a winter heat preservation device for dendrobium officinale planting, which comprises a fixing frame, a first supporting ring, a supporting frame and a film drawing mechanism, the fixing frame is of a rectangular frame body structure, a seedling raising groove is formed in the upper part of the fixing frame, and a material collecting groove is formed in the bottom of the seedling raising groove; a film pulling mechanism is arranged on the upper portion of the fixing frame and comprises a first connecting frame, a second connecting frame, a winding roller, a first material passing roller, a heat preservation film and a limiting clamping table, the first connecting frame is arranged on the side portion of the fixing frame, and the winding roller and the first material passing roller are arranged on the upper portion of the first connecting frame; a second connecting frame is arranged on a first fixing lug on the upper portion of the first connecting frame, and a second material passing roller is arranged on the second connecting frame. A limiting clamping table is arranged on the other side of the fixing frame, and a magnetic plate is arranged in a bearing groove of the limiting clamping table. According to the heat preservation device, the heat preservation film can be laid conveniently, the heat preservation device is convenient to disassemble, and seedling raising efficiency is improved.

The invention discloses a microbial agent composition beneficial to foliage spraying of dendrobium nobile, and relates to the technical field of agricultural microbial application. Dendrobium nobile endophytic phyllosticta is adopted as a core bacterium, bacillus amyloliquefaciens and streptomyces jingyangensis are compounded, and dendrobium nobile stem extract and jasmonic acid methyl ester are matched to serve as dual inducers; the preparation method comprises the following steps: constructing an integrated auxiliary agent system consisting of a chitosan-nano-selenium compound, erythritol and vitamin C; designing a special nutritional auxiliary material containing chelated calcium and magnesium, dendrobium nobile leaf superfine powder and betaine; the finished product is prepared by the processes of independently propagating strains, mixing the strains in sequence, compounding additives in stages and lagging in addition of nutritional auxiliary materials. The composition provided by the invention can improve the colonization rate of the strain on the lignified leaves of the dendrobium nobile, enhance the nutrient penetration and absorption capability, and directionally promote the accumulation of dendrobine, also gives consideration to the effects of growth promotion and disease resistance, and meets the medicinal cultivation requirements of the dendrobium nobile.