Preparation method of small molecular weight dendrobium candidum polysaccharides

A technology of Dendrobium officinale polysaccharide and small molecular weight, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as inflammation, harmful microorganisms eroding skin, and skin barrier function disappearance.

Active Publication Date: 2017-06-20
PROYA COSMETICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the skin hygiene is good, the normal flora is balanced, the probiotics are dominant, and the metabolites (sebum, sweat) on the skin are mostly used by the beneficial flora, which promotes the pH drop and the formation of the skin barrier, which can inhibit harmful bact

Method used

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  • Preparation method of small molecular weight dendrobium candidum polysaccharides
  • Preparation method of small molecular weight dendrobium candidum polysaccharides
  • Preparation method of small molecular weight dendrobium candidum polysaccharides

Examples

Experimental program
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Effect test

Embodiment 1

[0047] A. Strain activation and expansion: Under sterile conditions, use a sterile dropper to draw an appropriate amount of liquid medium and drop it into the strain tube containing the endophytic fungus Fusarium oats freeze-dried powder of Dendrobium officinale to prepare a strain concentration of 1× 10 6 CFU / mL bacterial suspension, then take 0.2mL of bacterial suspension and streak on the surface of PDA solid medium, culture at 25°C for 48h, pick a well-dispersed single colony and inoculate it into a fermentation shaker flask containing liquid medium components Carry out expansion cultivation in 25°C, 200r / min under the condition of oxygen supply for 48h, the oxygen concentration is 0.030mol / L, and the fermented seed liquid is obtained, in which the concentration of Fusarium avenatum is 1×10 5 CFU / mL, the liquid medium used is MS liquid medium containing 30.0g / L sucrose and 8.0g / L yeast powder;

[0048] B, treatment of dendrobium candidum broken wall: under the condition...

Embodiment 2

[0053] A. Strain activation and expansion: Under sterile conditions, use a sterile dropper to draw an appropriate amount of liquid medium and drop it into the strain tube containing the endophytic fungus Fusarium oats freeze-dried powder of Dendrobium officinale to prepare a strain concentration of 8.5× 10 5 CFU / mL bacterial suspension, then take 0.4mL bacterial suspension and streak on the surface of PDA solid medium, culture at 20°C for 36h, pick a well-dispersed single colony and inoculate it into a fermentation shaker flask containing liquid medium components Carry out expansion culture in 20°C and 200r / min under the condition of oxygen supply for 72h, the oxygen concentration is 0.010mol / L, and the fermented seed liquid is obtained, in which the concentration of Fusarium avenatum is 6.5×10 4 CFU / mL, the liquid medium used is MS liquid medium containing 18.0g / L sucrose and 10.0g / L yeast powder;

[0054] B, Dendrobium officinale wall breaking treatment: under the condition...

Embodiment 3

[0059] A. Strain activation and expansion: Under sterile conditions, use a sterile dropper to draw an appropriate amount of liquid medium and drop it into the strain tube containing the endophytic fungus Fusarium oats freeze-dried powder of Dendrobium officinale to prepare a strain concentration of 1× 10 5 CFU / mL bacterial suspension, then take 0.5mL bacterial suspension and streak on the surface of PDA solid medium, culture at 30°C for 24 hours, pick a well-dispersed single colony and inoculate it into a fermentation shaker flask containing liquid medium components Carry out expansion culture in the middle, 30 ℃, 200r / min conditions of oxygen supply culture 24h, oxygen concentration 0.020mol / L, make fermented seed liquid, wherein the concentration of Fusarium avenatum is 1×10 4 CFU / mL, the liquid medium used is MS liquid medium containing 15.0g / L sucrose and 10.0g / L yeast powder;

[0060] B. Treatment of dendrobium candidum wall breaking: at 51°C, pre-mix 3kg of dendrobium p...

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Abstract

The invention relates to a preparation method of small molecular weight dendrobium candidum polysaccharides. The preparation method comprises following steps: A, bacterial strain activation and expanding culture are carried out, wherein under aseptic conditions, an appropriate amount of a liquid culture medium is used for preparing a fusarium avenaceum bacterium suspension; B, high pressure homogenization is repeated for 5 to 10 times using a high pressure micro jet homogenizer so as to obtain a dendrobium candidum wall-broken homogenate; C, fermentation culturing is carried out, wherein an appropriate amount of a liquid culture medium is injected into a fermentation tank, the dendrobium candidum wall-broken homogenate and the fusarium avenaceum bacterium suspension are weighed, and are delivered into the fermentation tank for fermentation so as to obtain a fermentation liquid; and D, polysaccharide purification is carried out, wherein after fermentation, active carbon is used for decolouring, an obtained product is allowed to stand for 1h for absorption, filtering is carried out so as to remove residue and obtain a filtrate, vacuum pumping filtration is carried out so as to obtain a precipitate, the precipitate is washed with ethanol for three times, and vacuum drying is carried out so as to obtain the small molecular weight dendrobium candidum polysaccharides. The average molecular weight of the small molecular weight dendrobium candidum polysaccharides is lower than 300kDa; and the small molecular weight dendrobium candidum polysaccharides are capable of promoting propagation of skin probiotics, increasing the density of langerhans cells on skin, and improving skin immunity.

Description

technical field [0001] The invention relates to the technical field of extracting effective components of natural products, in particular to a preparation method of polysaccharides of dendrobium officinale with small molecular weight. Background technique [0002] Dendrobium candidum Wall.ex Lindl is a perennial epiphytic herb of the Orchidaceae genus Dendrobium. It is rich in Dendrobium polysaccharides, dendrobine, amino acids, phenanthrene compounds and various trace elements. precious Chinese herbal medicines. Dendrobium officinale is mostly used in the health care industry. The main active ingredient is Dendrobium polysaccharides. Oral administration can enhance the body's immunity, anti-tumor, anti-oxidation and delay aging. At present, the cosmetics industry has also begun to use Dendrobium officinale polysaccharides as cosmetic raw materials, but most of them are based on the long-lasting moisturizing effect of Dendrobium officinale polysaccharides with large molecul...

Claims

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Application Information

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IPC IPC(8): C12P19/04C08B37/00C12R1/77
CPCC08B37/0003C12P19/04
Inventor 薛绘张金龙王丽娜杨盼盼蒋丽刚毕永贤李文倩
Owner PROYA COSMETICS
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