21 results about "Dendrobine" patented technology

The invention discloses endophyte Schizophyllanthus commune CY01-1H for improving synthesis of jasmonic acid and dendrobine of dendrobium nobile seedlings and application of the endophyte Schizophyllanthus commune CY01-1H, and belongs to the technical field of plant secondary metabolism regulation and control and microorganisms. The preservation number of the endophyte CY01-1H is CGMCC (China General Microbiological Culture Collection Center) No.42003; the strain is preserved in China General Microbiological Culture Collection Center on May 12, 2025, and the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the endophytic fungus CY01-1H obtained through separation comes from a healthy dendrobium nobile plant, the content of jasmonic acid of dendrobium nobile and accumulation of dendrobine can be increased by planting the endophytic fungus CY01-1H in dendrobium nobile, the normal growth state of the plant is not affected, and wide application prospects are brought for improving the quality of the dendrobium nobile medicinal material.

The invention provides a POCD treatment medicine composition with dendrobine delivered by a nano-carrier and application, and belongs to the technical field of medicine. The composition consists of dendrobine, a nano-carrier material and a stabilizer, wherein a nano-carrier is PLGA (poly (lactic-co-glycolic acid)) nanoparticles or lipidosome. The particle size of the composition is 50-150nm, the polydispersity index is less than 0.3, and the loading capacity of dendrobine is 5-15%. The invention also provides two preparation methods of the composition. The PLGA nanoparticles are prepared by adopting an emulsification-solvent evaporation method; the liposome is prepared by adopting a film hydration-ultrasonic extrusion method, and each process parameter is specifically limited. The pharmaceutical composition can significantly improve the brain targeting and bioavailability of dendrobine, and can be used for preparing drugs for preventing or treating postoperative cognitive impairment (POCD), and the administration route can be intravenous injection or nasal administration.

An endophytic fungus of Dendrobium nobile and a use thereof provided. The endophytic fungus is obtained by separating a living Orchidaceae perennial herb, Dendrobium nobile, by adopting an endophytic fungus separation and purification technology, with a category name of Phyllosticta fallopiae DNL19, which has been deposited in the China General Microbiological Culture Collection Center on Apr. 27, 2023, with a strain deposit number of CGMCC No. 40583. Meanwhile, a use of the endophytic fungus is provided, which promotes the growth of Dendrobium nobile, as well as the accumulation of an effective constituent dendrobine. The endophytic fungus brings broad application prospects in the field of yield and quality improvement of Dendrobium nobile medicinal materials.

The invention belongs to the technical field of liquid fermentation of endophytic fungi, and particularly relates to a method for producing dendrobine by co-culture of endophytic fungi and dendrobium nobile and a special strain. According to the method, fusarium endophytic fungi which are preserved in the China General Microbiological Culture Collection Center (CGMCC) and have the preservation number of CGMCC NO.3.27824 are adopted, dendrobium nobile stems or dry powder is added into an optimized liquid culture medium to serve as a homologous stimulant for co-culture, and the yield of dendrobine is remarkably increased. Experiments show that by means of the method, the yield of dendrobine can be increased by dozens of times or above and up to about 99 times, the process is stable, cost is low, and large-scale production is easy to achieve. The invention provides a new way for large-scale green production of dendrobine, and reduces the dependence on wild dendrobium resources.

The invention discloses a novel application of VCH-916 in increasing the content of dendrobine in dendrobium nobile lindl. Belongs to the field of plant biotechnology. By applying an effective dose of VCH-916 to dendrobium nobile, a dendrobine biosynthesis pathway of dendrobium nobile can be remarkably activated, so that the accumulation amount of dendrobine is greatly increased. The method is easy and convenient to operate, remarkable in effect and high in safety, and has great significance in solving the problem of shortage of high-quality dendrobium nobile resources and improving the medicinal material value and economic benefits of the dendrobium nobile.

The invention discloses a dendrobium nobile endophytic fungus for producing dendrobine and application of the dendrobium nobile endophytic fungus. The method aims at solving the problems that in an existing plant, the dendrobine content is low, and the culture period is long. The dendrobium nobile endophytic fungus is fusarium 689, and is preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 21, 202 5, and the preservation number is CGMCC 3.27824. The method for producing dendrobine by using the strain comprises the following steps: carrying out dark culture on preserved hyphae in an artificial climate box at 25 DEG C for 12 hours, then picking up the hyphae, inoculating the hyphae into a new solid PDA culture medium, carrying out dark culture at 25 DEG C for several days, picking up the hyphae, inoculating the hyphae into a 100ml liquid PDB culture medium, culturing in a culture bottle in a dark environment at the speed of 120 rpm and the temperature of 25 DEG C for about 30 days, and separating fermentation liquor from thalli (dried), so as to obtain the dendrobine. And detecting dendrobine from the thalli. The strain has good antioxidant enzyme activity and can also be applied to production of dendrobine through microbial fermentation.

The invention provides a preparation method and application of superfine crushed dendrobium nobile, and belongs to the technical field of traditional Chinese medicine processing and application, the preparation method comprises the following steps: 1) airing fresh dendrobium nobile stems, cutting into segments, and drying to obtain dry stem segments; 2) pre-crushing the dried stem segments, performing first screening, and collecting undersize components to obtain fine powder; and (3) carrying out superfine grinding on the fine powder, carrying out secondary sieving, and collecting undersize components as superfine powder. According to the preparation method disclosed by the invention, the structure of the dendrobium nobile is greatly destroyed, the particle size of the powder is reduced, the cell wall breaking rate is increased, and the leaching and release of active ingredients such as polysaccharides, dendrobine, total polyphenols and total flavonoids in the dendrobium nobile are promoted; compared with conventional fine powder, the dendrobium nobile submicron powder prepared by the preparation method disclosed by the invention has the advantage that the liver protection activity of dendrobium nobile can be remarkably enhanced.

This invention relates to the field of breast cancer therapeutics, providing a novel application of *Dendrobium nobile*, specifically the application of dendrobine in the preparation of breast cancer therapeutics. This invention verifies that dendrobine can significantly inhibit breast cancer cell viability. The inhibitory effect of dendrobine on the migration and proliferation of T47D breast cancer cells and its ability to induce cell death increases with increasing dosage. Simultaneously, cloning experiments also show that dendrobine significantly inhibits the colony-forming ability of breast cancer cells. ROS and JC-1 experiments also show that dendrobine induces mitochondrial dysfunction and increases ROS levels. Western blot experiments show that under dendrobine treatment, the expression levels of ferroptosis markers GPX4 and Nrf2 are significantly reduced. Dendrobine can promote ferroptosis in breast cancer cells and inhibit their cell viability by inducing mitochondrial dysfunction, and it holds promise as a breast cancer therapeutic drug specifically targeting ferroptosis.

The present application belongs to the field of microbial technology, and particularly relates to a bacillus subtilis strain, a fermentation method thereof and application. The present application isolates a bacillus subtilis strain with high cellulase yield from medicinal plants Dendrobium, which has been preserved in China General Microbiological Culture Collection Center (CGMCC) on September 19, 2016, and the address is No. 1, Yihuangyuan 3rd, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 13010. The strain can produce cellulase with high activity, can decompose cellulose in the cell wall of Dendrobium, and promote the dissolution of effective components such as Dendrobium alkaloids. The Dendrobium fermented by the strain can improve the growth performance and immune function of South American white prawn, large yellow croaker and other aquatic animals.

The invention discloses a multi-index quality analysis method of dendrobium nobile and application of the multi-index quality analysis method. According to the invention, a high performance liquid chromatography (HPLC) method is adopted to establish a method for determining the content of Dendrobin A, xanthophyll, New Zealand vitexin and isophorone in stems and flowers of Dendrobium nobile; a method for determining the content of dendrobine is established by adopting a gas chromatography (GC) method, and a method for determining the content of large components including total polyphenols, total flavonoids, total anthocyanin, total polysaccharides and total alkaloids in stems and flowers of dendrobium nobile lindl is established by adopting an ultraviolet-visible (UV-Vis) spectrophotometric method. According to the quality standard disclosed by the invention, systematic and comprehensive quality control is carried out on the dendrobium nobile for the first time, and the quality standard not only has an important guiding significance on establishing the quality standard of the dendrobium nobile medicinal material red water in Guizhou, but also has an important guiding effect on research of a dendrobium nobile extraction process and development and quality control of related products.

The invention discloses application of dendrobine combined with lactobacillus paracasei, and particularly relates to application of dendrobine combined with lactobacillus paracasei in preparation of products for preventing and / or relieving learning and memory ability damage induced by high-sugar diet. Experiments prove that the combination of the dendrobine and the lactobacillus paracasei increases the variety diversity of intestinal flora, improves the glucose and lipid metabolism disorder of rats induced by high glucose, improves the abnormal blood fat and blood glucose, can prevent and / or improve the influence of the high glucose on the learning and memory ability of the rats, and has a good synergistic effect compared with the single use of the dendrobine or the lactobacillus paracasei. The combined use of dendrobine and lactobacillus paracasei also avoids the problem that the influence effect on learning and memory ability is not obvious when the dendrobine and lactobacillus paracasei are independently used, and the dendrobine and lactobacillus paracasei composition is effective and safe and has very high clinical application value.

The present invention discloses a dendrobium, a pharmaceutical composition and its use in treating diseases related to STAT3 abnormality, specifically the use of dendrobium in preparing a medicament for treating diseases related to STAT3 (signal transduction and activator of transcription 3) abnormality, especially the use of dendrobium in treating inflammatory diseases including sepsis, progressive inflammatory bowel disease, chronic obstructive pulmonary disease, bronchial asthma, chronic nephritis, gastritis, pancreatitis, obstructive nephropathy, diabetic glomerulopathy, retinitis and the like. The present invention is the first to discover that dendrobium has an inhibitory effect on STAT3, which can not only expand the application field of dendrobium, make traditional Chinese medicinal materials realize greater value, but also provide new drugs for treating diseases related to STAT3. The present invention finds that the combination of dendrobium and anti-inflammatory drugs, especially natural medicines such as ligustrazine, resveratrol, limonene, and matrine, has a good synergistic effect and can greatly improve the anti-inflammatory effect.

The invention discloses a dendrobium officinale primary pulp beverage which comprises tremella pulp, dendrobium juice, dendrobium primary pulp, fructo-oligosaccharide, zero-calorie sugar and water. The mass ratio of the raw materials of the tremella pulp: water: crushed tremella is 2: 1; the dendrobium nobile juice is prepared from the following raw materials: dendrobium nobile, tremella pulp and water in a mass ratio of 1: (2-5): (15-18). The raw materials of the dendrobium nobile primary pulp comprise dendrobium nobile juice, fructo-oligosaccharide and zero-calorie sugar in a mass ratio of 100: 1: (0.3-1); the zero-calorie sugar is formed by compounding erythritol and sucralose according to the mass ratio of 9: 1, the erythritol accounts for 90% to neutralize the metal aftertaste of the sucralose and provide a cool taste, and the sucralose accounts for 10% to activate a sweet taste receptor and enhance the aftertaste level; according to the method disclosed by the invention, a low-temperature crushing technology and a high-pressure sol technology are creatively fused, and liquid chromatography-mass spectrometry (LC-MS) detection proves that the dendrobine content is increased by 12% compared with that of a conventional method.

The present application belongs to the technical field of biological medicine, and particularly relates to the application of dendrobium alkaline and / or pinoecorine in the treatment of depression. Specifically, the present application proves through experiments that dendrobium alkaline and pinoecorine can improve LPS-induced depressive behavior, and reduce the level of oxidative stress and neuroinflammatory response by targeting PDE4B to inhibit the activation of the TLR4 / PI3K / AKT / NF-κB pathway, thereby reversing the damage of neurotoxicity, apoptosis abnormality and synaptic plasticity to relieve symptoms. Dendrobium alkaline improves the intestinal flora disorder, and acts on the central nervous system through the microbe-gut-brain axis, so that the anti-depression effect of dendrobium alkaline is better than that of pinoecorine. The present application opens up a new drug use for dendrobium alkaline and pinoecorine, and lays an experimental foundation and provides a new perspective for the development of efficient drugs for treating depression.

The present invention discloses a dendrobium alkaloid small molecule active probe and a preparation method, belonging to the field of biomedicine technology. The chemical structure of the dendrobium alkaloid small molecule active probe is as follows: The dendrobium alkaloid small molecule active probe is used to determine the direct target of dendrobium alkaloid. Experimental results show that the dendrobium alkaloid small molecule probe synthesized for the first time has biological activity similar to that of dendrobium alkaloid and can specifically bind to the target of dendrobium alkaloid. This proves that it can be used as a tool to replace dendrobium alkaloid in binding research on related protein targets, fills the gap in the existing technology, and provides a basis for in-depth research on the mechanism of action of dendrobium alkaloid.

The utility model discloses wall breaking equipment for fresh dendrobium huoshanense freeze-dried powder, and relates to the technical field of dendrobium processing equipment. The device comprises an outer cylinder and an inner cylinder, the inner cylinder is rotationally connected to the interior of the outer cylinder, supporting plates are fixedly connected to the two sides of the bottom of the outer cylinder, and a transverse plate is fixedly connected between the two supporting plates; a cold circulation mechanism is arranged at the top of the transverse plate and comprises a cold water tank fixedly connected to the top of the transverse plate, a circulating water pump communicating with one side of the cold water tank and a circulating water pipe communicating with the top of the circulating water pump. Cold water in a cold water tank is pumped into a circulating water pipe through a circulating water pump in the cold circulating mechanism, heat on the outer wall of the inner barrel is taken away through continuous circulation of the cold water, and the heat can be discharged through vent holes by matching with rotating fan blades in the driving mechanism, so that the problem of heat accumulation in the inner barrel is solved, and the service life of the inner barrel is prolonged. And the activity of dendrobine is reduced.

The invention belongs to the field of cell in-vitro culture, and particularly discloses an application of sulfydryl modified dendrobine in oocyte in-vitro maturation culture, a sulfydryl (-SH) functional group is introduced on a dendrobine molecular structure through chemical synthesis to obtain modified dendrobine, the modified dendrobine is added into an IVM culture medium, and the oocyte in-vitro maturation culture is obtained. A newly introduced sulfydryl (-SH) functional group can be directly subjected to redox reaction with ROS (such as superoxide anions and hydroxyl radicals), so that the free radical scavenging capacity is enhanced, and the ROS is reduced; the GSH level can be obviously improved; the oxidative stress of porcine oocytes can be effectively relieved, the early cell apoptosis level is reduced, the mitochondrial membrane potential is maintained, and the blastocyst rate is increased.

The invention discloses a microbial agent composition beneficial to foliage spraying of dendrobium nobile, and relates to the technical field of agricultural microbial application. Dendrobium nobile endophytic phyllosticta is adopted as a core bacterium, bacillus amyloliquefaciens and streptomyces jingyangensis are compounded, and dendrobium nobile stem extract and jasmonic acid methyl ester are matched to serve as dual inducers; the preparation method comprises the following steps: constructing an integrated auxiliary agent system consisting of a chitosan-nano-selenium compound, erythritol and vitamin C; designing a special nutritional auxiliary material containing chelated calcium and magnesium, dendrobium nobile leaf superfine powder and betaine; the finished product is prepared by the processes of independently propagating strains, mixing the strains in sequence, compounding additives in stages and lagging in addition of nutritional auxiliary materials. The composition provided by the invention can improve the colonization rate of the strain on the lignified leaves of the dendrobium nobile, enhance the nutrient penetration and absorption capability, and directionally promote the accumulation of dendrobine, also gives consideration to the effects of growth promotion and disease resistance, and meets the medicinal cultivation requirements of the dendrobium nobile.

The invention discloses a traditional Chinese medicine formula for treating gastric cancer as well as a preparation method and application thereof. The formula is prepared from the following components in parts by weight: monarch drugs: 38-90 parts of tripterygium wilfordii alkaloid, 38-90 parts of tripterygium hypoglaucum alkaloid, 28-85 parts of chamaejasmine, 28-85 parts of gelsemium elegans alkaloid and 28-85 parts of xanthoxylin; ministerial and adjuvant drugs: 16 to 80 parts of ganoderan, 16 to 80 parts of pinellia ternate sitosterol, 16 to 80 parts of radix rehmanniae praeparata catalpol, 16 to 80 parts of amomum cardamomum volatile oil, 16 to 80 parts of piper cubeba element, 16 to 80 parts of dendrobine, 16 to 80 parts of alpinia katsumadai volatile oil, 16 to 80 parts of panax notoginseng ginsenoside, 16 to 80 parts of linalool, 16 to 80 parts of caulis spatholobi element, 16 to 80 parts of cassia seed element, 16 to 80 parts of bergamot alcohol, 16 to 80 parts of cistanche deserticola, 16 to 80 parts of fructus forsythiae alkaloid and 16 to 80 parts of mulberry inorganic salt; 16 to 80 parts of total flavonoids of sea-buckthorn; and 100 parts of water. Wherein the ratio of the total parts by weight of the monarch drugs to the total parts by weight of the ministerial and adjuvant drugs is 1: (1.5-5.0). The traditional Chinese medicine formula for treating gastric cancer provided by the invention has the advantages of obvious curative effect, multi-target synergistic effect, high safety and controllable toxicity, and can improve the living quality of patients.

The invention relates to a method for improving growth of dendrobium officinale seedlings. The dendrobium officinale seedlings are illuminated through combined light waves. The method has the advantages that growth is promoted, the increase of the content of polysaccharide, mannose and dendrobine of dendrobium officinale seedlings is remarkably influenced, and the content of endogenous auxin is increased.

The invention provides multi-effect application of bletilla chinensis callus extract in the field of cosmetics, and belongs to the technical field of cosmetic raw material development. The invention provides application of bletilla chinensis callus extract in resisting aging, repairing skin barrier, moisturizing, relieving, resisting light aging, repairing DNA and balancing cell cycle, the extract has rare bibenzyl substances, such as 3, 3 '-dihydroxy-2-p-hydroxybenzyl-5-methoxy bibenzyl, 3, 3', 5-trihydroxy bibenzyl, 3, 3 '-dihydroxy-5-methoxy bibenzyl, dimethoxyl bibenzyl, dimethoxyl bibenzyl, dimethoxyl bibenzyl, dimethoxyl bibenzyl, dimethoxyl bibenzyl, dimethoxyl bibenzyl, dimethoxyl bibenzyl, dimethoxyl bibenzyl, dimethoxyl bibenzyl, dimethoxyl bibenzyl, dimethoxyl bibenzyl, dimethoxyl bibenzyl, dimethoxyl bibenzyl, dimethoxyl bibenzyl, dimethoxyl bibenzyl, dimethoxyl bibenzyl The compound is prepared from the following raw materials: 3, 3 '-dihydroxy-5-methoxy bibenzyl), dendrophenol (3, 3'-dihydroxy-5, 4 '-dimethoxy bibenzyl), Dactylorin E and the like; the bletilla chinensis callus extract has excellent effects in the aspects of aging resistance, skin barrier repairing, soothing, light aging resistance, DNA repairing and cell cycle balancing, the application of the bletilla chinensis callus extract in cosmetics is developed, a new raw material is provided for the field of cosmetics, and the bletilla chinensis callus extract has a wide application prospect.