8 results about "Dendrobine" patented technology

The invention belongs to the technical field of liquid fermentation of endophytic fungi, and particularly relates to a method for producing dendrobine by co-culture of endophytic fungi and dendrobium nobile and a special strain. According to the method, fusarium endophytic fungi which are preserved in the China General Microbiological Culture Collection Center (CGMCC) and have the preservation number of CGMCC NO.3.27824 are adopted, dendrobium nobile stems or dry powder is added into an optimized liquid culture medium to serve as a homologous stimulant for co-culture, and the yield of dendrobine is remarkably increased. Experiments show that by means of the method, the yield of dendrobine can be increased by dozens of times or above and up to about 99 times, the process is stable, cost is low, and large-scale production is easy to achieve. The invention provides a new way for large-scale green production of dendrobine, and reduces the dependence on wild dendrobium resources.

The invention discloses a novel application of VCH-916 in increasing the content of dendrobine in dendrobium nobile lindl. Belongs to the field of plant biotechnology. By applying an effective dose of VCH-916 to dendrobium nobile, a dendrobine biosynthesis pathway of dendrobium nobile can be remarkably activated, so that the accumulation amount of dendrobine is greatly increased. The method is easy and convenient to operate, remarkable in effect and high in safety, and has great significance in solving the problem of shortage of high-quality dendrobium nobile resources and improving the medicinal material value and economic benefits of the dendrobium nobile.

This invention relates to the field of breast cancer therapeutics, providing a novel application of *Dendrobium nobile*, specifically the application of dendrobine in the preparation of breast cancer therapeutics. This invention verifies that dendrobine can significantly inhibit breast cancer cell viability. The inhibitory effect of dendrobine on the migration and proliferation of T47D breast cancer cells and its ability to induce cell death increases with increasing dosage. Simultaneously, cloning experiments also show that dendrobine significantly inhibits the colony-forming ability of breast cancer cells. ROS and JC-1 experiments also show that dendrobine induces mitochondrial dysfunction and increases ROS levels. Western blot experiments show that under dendrobine treatment, the expression levels of ferroptosis markers GPX4 and Nrf2 are significantly reduced. Dendrobine can promote ferroptosis in breast cancer cells and inhibit their cell viability by inducing mitochondrial dysfunction, and it holds promise as a breast cancer therapeutic drug specifically targeting ferroptosis.

The present application belongs to the field of microbial technology, and particularly relates to a bacillus subtilis strain, a fermentation method thereof and application. The present application isolates a bacillus subtilis strain with high cellulase yield from medicinal plants Dendrobium, which has been preserved in China General Microbiological Culture Collection Center (CGMCC) on September 19, 2016, and the address is No. 1, Yihuangyuan 3rd, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 13010. The strain can produce cellulase with high activity, can decompose cellulose in the cell wall of Dendrobium, and promote the dissolution of effective components such as Dendrobium alkaloids. The Dendrobium fermented by the strain can improve the growth performance and immune function of South American white prawn, large yellow croaker and other aquatic animals.

The present application belongs to the technical field of biological medicine, and particularly relates to the application of dendrobium alkaline and / or pinoecorine in the treatment of depression. Specifically, the present application proves through experiments that dendrobium alkaline and pinoecorine can improve LPS-induced depressive behavior, and reduce the level of oxidative stress and neuroinflammatory response by targeting PDE4B to inhibit the activation of the TLR4 / PI3K / AKT / NF-κB pathway, thereby reversing the damage of neurotoxicity, apoptosis abnormality and synaptic plasticity to relieve symptoms. Dendrobium alkaline improves the intestinal flora disorder, and acts on the central nervous system through the microbe-gut-brain axis, so that the anti-depression effect of dendrobium alkaline is better than that of pinoecorine. The present application opens up a new drug use for dendrobium alkaline and pinoecorine, and lays an experimental foundation and provides a new perspective for the development of efficient drugs for treating depression.

The utility model discloses wall breaking equipment for fresh dendrobium huoshanense freeze-dried powder, and relates to the technical field of dendrobium processing equipment. The device comprises an outer cylinder and an inner cylinder, the inner cylinder is rotationally connected to the interior of the outer cylinder, supporting plates are fixedly connected to the two sides of the bottom of the outer cylinder, and a transverse plate is fixedly connected between the two supporting plates; a cold circulation mechanism is arranged at the top of the transverse plate and comprises a cold water tank fixedly connected to the top of the transverse plate, a circulating water pump communicating with one side of the cold water tank and a circulating water pipe communicating with the top of the circulating water pump. Cold water in a cold water tank is pumped into a circulating water pipe through a circulating water pump in the cold circulating mechanism, heat on the outer wall of the inner barrel is taken away through continuous circulation of the cold water, and the heat can be discharged through vent holes by matching with rotating fan blades in the driving mechanism, so that the problem of heat accumulation in the inner barrel is solved, and the service life of the inner barrel is prolonged. And the activity of dendrobine is reduced.

The invention discloses a microbial agent composition beneficial to foliage spraying of dendrobium nobile, and relates to the technical field of agricultural microbial application. Dendrobium nobile endophytic phyllosticta is adopted as a core bacterium, bacillus amyloliquefaciens and streptomyces jingyangensis are compounded, and dendrobium nobile stem extract and jasmonic acid methyl ester are matched to serve as dual inducers; the preparation method comprises the following steps: constructing an integrated auxiliary agent system consisting of a chitosan-nano-selenium compound, erythritol and vitamin C; designing a special nutritional auxiliary material containing chelated calcium and magnesium, dendrobium nobile leaf superfine powder and betaine; the finished product is prepared by the processes of independently propagating strains, mixing the strains in sequence, compounding additives in stages and lagging in addition of nutritional auxiliary materials. The composition provided by the invention can improve the colonization rate of the strain on the lignified leaves of the dendrobium nobile, enhance the nutrient penetration and absorption capability, and directionally promote the accumulation of dendrobine, also gives consideration to the effects of growth promotion and disease resistance, and meets the medicinal cultivation requirements of the dendrobium nobile.

The invention discloses a traditional Chinese medicine formula for treating gastric cancer as well as a preparation method and application thereof. The formula is prepared from the following components in parts by weight: monarch drugs: 38-90 parts of tripterygium wilfordii alkaloid, 38-90 parts of tripterygium hypoglaucum alkaloid, 28-85 parts of chamaejasmine, 28-85 parts of gelsemium elegans alkaloid and 28-85 parts of xanthoxylin; ministerial and adjuvant drugs: 16 to 80 parts of ganoderan, 16 to 80 parts of pinellia ternate sitosterol, 16 to 80 parts of radix rehmanniae praeparata catalpol, 16 to 80 parts of amomum cardamomum volatile oil, 16 to 80 parts of piper cubeba element, 16 to 80 parts of dendrobine, 16 to 80 parts of alpinia katsumadai volatile oil, 16 to 80 parts of panax notoginseng ginsenoside, 16 to 80 parts of linalool, 16 to 80 parts of caulis spatholobi element, 16 to 80 parts of cassia seed element, 16 to 80 parts of bergamot alcohol, 16 to 80 parts of cistanche deserticola, 16 to 80 parts of fructus forsythiae alkaloid and 16 to 80 parts of mulberry inorganic salt; 16 to 80 parts of total flavonoids of sea-buckthorn; and 100 parts of water. Wherein the ratio of the total parts by weight of the monarch drugs to the total parts by weight of the ministerial and adjuvant drugs is 1: (1.5-5.0). The traditional Chinese medicine formula for treating gastric cancer provided by the invention has the advantages of obvious curative effect, multi-target synergistic effect, high safety and controllable toxicity, and can improve the living quality of patients.