100results about How to "Good wall breaking effect" patented technology

The invention relates to a method for preparing an ecological organic fertilizer from organic waste. The method comprises the following steps of putting the organic waste of which the moisture content is within 95% into a subcritical water reaction kettle, adding biomass which is 0-10% of the organic waste, feeding NaOH to the reaction kettle to adjust the pH to 7.5-8.5; leading steam into the reaction kettle, adjusting the temperature to 150-300 DEG C and the pressure to 0.5-5.0MPa to react for 15-60 minutes; baking or dewatering the product until the moisture content is 50-65%; feeding a microbial agent which is 0.5-1% of weight of the product to the baked or dewatered product; evenly mixing and then piling, heating the pile to 60-70 DEG C after 2-3 days to be stable, and carrying out heat preservation at 55-65 DEG C to maintain for 5-7 days, and turning when the temperature exceeds 65 DEG C and preparing the product until the temperature is not changed. By adopting the method, the limitation of the traditional aerobic fermentation is broken through; pathogene can be thoroughly killed; no bad smell or heavy metal pollution appears; the treatment cost is reduced; the treatment efficiency is improved; and the highest target of resource utilization of the organic waste is achieved.

The invention relates to a method for preparing natural carotenoid crystals, especially lycopene and beta-carotene by taking microorganism biomasses as raw materials. The method comprises the following steps of: adding an extraction solvent into Blakeslea trispora mycelium, carrying out cell disruption and filtering, and collecting filter liquor; carrying out vacuum concentration on the filter liquor, and recycling solvent to obtain oleoresin; adding a washing solvent into the oleoresin, and washing and filtering to obtain coarse crystals; and drying the coarse crystals and removing residual solvent, thus obtaining the high-content carotenoid crystals. According to the method provided by the invention, the types of the used solvents are few, the use levels of the used solvents are little, the extraction and purification processes are simple, the yield is high, and the method is suitable for industrial production; and the obtained carotenoid crystal product is high in content and good in quality, and can be widely applied to the fields of functional foods, medicines and the like.

The invention discloses device and method for cell wall breaking in a municipal sludge dewatering process. The device comprises an adjusting pond, a stirrer, a hot water tank, a hot water pump, a hot water pipe which is communicated with the adjusting pond through a heat exchanger, a sludge pump, a valve, a sludge inlet pipe which is communicated with the adjusting pond through the heat exchanger, an alkaline liquid tank, an alkaline liquid pump, an alkaline liquid pipe which is communicated with the adjusting pond, and a dewatering machine which is connected with the adjusting pond, wherein the filtrate from the dewatering machine is discharged through the heat exchanger, and the adjusting pond is provided with an alkaline liquid concentration sensor, a temperature sensor and a controller and is in connected connection with the sludge pump, a water pump, the alkaline liquid pump, the motor of the stirrer, the dewatering machine and the valve. The cell wall breaking method comprises the following steps of: (1) performing a pretest; (2) preparing an alkaline liquid; (3) loading sludge to the adjusting pond in which the sludge is preheated; (4) adding alkali to adjust the pH of the sludge and allowing mixed reactions to take place; (5) starting the dewatering machine and a filtrate return device; (6) detecting the sludge mixed reaction results at a preset time, to ensure optimum wall-breaking dewatering effect of the sludge; and (7) repeating from the step (3) to the step (6). The device and method provided by the invention are applied to the wall-breaking treatment prior to the dewatering treatment of the sludge, has a perfect structure, is easy to operate, and improves effects.

The invention provides a preparation method of wall-breaking enzymolysis bee pollen. The preparation method is characterized by specifically comprising the following steps: 1) removing impurities; 2) sterilizing; 3) mechanically breaking the wall; and 4) carrying out biological enzymolysis. The preparation method of the wall-breaking enzymolysis bee pollen has the following advantages that (1) the wall-breaking effect is remarkable, and the benefits are provided for releasing and separating the nutritional components in the bee pollen such as the fatty acid, flavonoid and polysaccharide; (2) the sensitization of antigen protein is removed, the enzymolysis is carried out on alkaline glutelin, and the bioavailability is improved; (3) the dietary fibers such as lignin are obviously reduced, the absorption performance of animals to liposoluble substances is improved, the energy utilization rate is raised, and the animal production achievement is improved; (4) the particles are fine, good in flavor and comfortable in taste, and the animal ingestion and favorite are obviously improved; and (5) the action condition is mild, and little damage is brought to the heat sensitive nutritional substances.

A process for fermenting the fresh flower of marigold by bacterium lactis includes such steps as drying in the air, screening, loading it in fermenting pool, uniformly spraying composite bacterium lactis, compacting, sealing, and anaerobic fermenting at medium temp for 8-12 days. Its advantages are high acid generating speed and wall-breaking effect, and unique taste.

A kind of animals and plants and microbial cell wall dissolving enzymes reaction solutionú¼which belongs to enzyme dissolving technique of cell engineering. The character is: the reaction solution includes cell wall dissolving enzymes, protoplasm solution, enzymes promoter, inorganic salt, mineral water and other ingredients. The cell wall dissolving enzyme reaction solution isní»t poisonous and has good biological degradation ability. As a result of fermenting of animals and plants and microorganism, the molecular structure of the product has changed completely. The mass of biological active substance such as small cell protein, free amino acid, linolenic acid and polypeptide increase a lot. Also, these substances can be absorbed easily by human.

The invention discloses a Chinese herbal piece ultrasonic wall breaking method. The Chinese herbal piece ultrasonic wall breaking method comprises the following steps: 1) pretreatment: Chinese herbal medicines are put in a drying chamber from a feed port to obtain dried Chinese herbal medicines; 2) the dried Chinese herbal medicines obtained in the step 1) are roughly crushed by a 80-mesh rough crushing device to obtain rough powder of 80-100 meshes; 3) ultrasonic wall breaking: the rough powder obtained in the step 2) is controlled through a flow valve to add in a first wall breaking reaction cavity for ultrasonic crushing to reach a particle size of 10-100 microns so as to obtain wall breaking fine powder; 4) secondary ultrasonic wall breaking: the wall breaking fine powder obtained in the step 3) is added in a second wall breaking reaction cavity for secondary wall breaking by 30-90 min to obtain secondary wall breaking powder; and 5) ternary ultrasonic wall breaking: the secondary wall breaking powder is put in a third wall breaking reaction cavity for ultrasonic wall breaking by at least 20 min to obtain wall breaking ultrafine powder; and the wall breaking ultrafine powder flows out from a discharge port. The Chinese herbal piece ultrasonic wall breaking method can be applied to manufacture ultrafine wall breaking Chinese herbal pieces, and is better in wall breaking effect of the Chinese herbal pieces through three times of continuous ultrasonic wall breaking.

The invention discloses a preparation method of an alga natural antioxidant. The preparation method is as follows: beating and crushing fresh alga, extracting with hot water, centrifuging, drying, crushing, sieving to obtain alga residue, further using a biological enzyme method for broking wall to obtain wall-broken alga residue dry powder; using food grade absolute ethanol for extraction at room temperature, collecting an extracting solution, and performing decompression concentration and vacuum drying to obtain the natural algae antioxidant product. The natural algae antioxidant product is paste, and comprises 5.2 to 13.7 by mass% of total phenol, 3.6 to 8.4 by mass% of total carotenoids, 0.5 to 1.3 by mass% of terpenoids and 0.9 to 2.6 by mass% of sterol compounds. The preparation method adopts the alga residue as a raw material to prepare the natural antioxidant with strong antioxidant activity, realizes the comprehensive utilization of resources, is in simple, efficient, green and environmentally friendly in the production process, and is suitable for industrial production; the natural algae antioxidant product is strong in antioxidant capacity, natural and green, and can replace traditional chemical synthesized antioxidants to use in food industry.

The invention discloses a method for extracting an active polysaccharide from the seaweed, which comprises the steps of seaweed cleaning, seaweed crushing treatment, seaweed degreasing treatment, enzymolysis treatment, super extraction, concentration, protein removal treatment, decolorization treatment, alcohol precipitation and the like. The method for extracting an active polysaccharide from theseaweed comprises the following steps of: firstly carrying out superfine grinding on the seaweed, extracting the polysaccharide of the seaweed by adopting a mode of combining microwave-assisted enzymolysis and super extraction, thereby achieving a good cell wall breaking effect, effectively improving the leaching rate of the polysaccharide and the extraction efficiency is greatly improved, the process route is simple, and the production cost is low. The method for extracting an active polysaccharide from the seaweed is simple and feasible, the extraction conversion rate and the purity of thepolysaccharide are higher, the industrial production is easy with higher safety, and the economic value of the seaweed is greatly improved., The method has mild deproteinization and decolorization processes, shortens the process flow, reduces the energy consumption and the consumption of organic solvents, which is more suitable for large-scale production and application.

The invention relates to the technical field of traditional Chinese medicinal material processing, in particular to a preparation method of directly orally-taken traditional Chinese medicinal decoction dendrobium powder. The preparation method comprises the following steps: (1) sterilizing: putting dendrobium into ozone water for performing ozone sterilization; (2) crushing: crushing the dendrobium by a wet crushing method to obtain a dendrobium slurry; (3) filtering: performing vacuum suction filtration on the dendrobium slurry by using a filter screen to obtain a filtrate slurry; (4) concentrating: performing vacuum reduced pressure concentration on the filtrate slurry to obtain a concentrated slurry; (5) drying: performing vacuum freeze drying on the concentrated slurry to obtain the dendrobium powder. A finished product prepared by the preparation method is extremely fine powder which is 100% broken, and has good water solubility; the sterilizing effect is good; active components are basically not destroyed; the finished product is high in active component polysaccharide content, low in moisture content, sterile and long in shelf life.

The invention discloses a preparation method of high-purity all-trans lycopene crystal, and comprises the following steps: filtering the ferment material liquid, thus obtaining mycelium; adding polar organic solvent to the mycelium to grind and break the wall, and then filtering and removing the organic solvent; taking the mixture of mycelium and low-boiling petroleum based organic solvent as degreaser to remove mycelium oily consistituent and the foreign matter such as little amount of Beta-carotene; taking the mixture of mycelium and low-boiling petroleum based organic solvent as extractingagent to liquid-solid extract the lycopene in mycelium; cooling down the extract liquor for crystallization; filtering and drying in vacuum, thus obtaining high-purity all-trans lycopene crystal. Thetechnique adopted by the method can avoid lycopene from retrogradation and isomerization owing to heat and oxidation. The lycopene crystal prepared by the method has high purity, the content of lycopene exceeds over 97 percent, and the content of the all-trans structure exceeds over 90 percent.

The invention provides a novel method for producing yeast autolysate by probiotic fermentation, which is particularly implemented by carrying out mixed fermentation on Bacillus subtilis K1 and yeast cells. The collection number of the Bacillus subtilis K1 is CCTCC NO:M2014396. After 48 hours of fermentation, the wall breaking ratio of the yeast cells in the fermentation tank filled with the Bacillus subtilis K1 is up to 95% above, thereby obtaining unexpected technical effects. Compared with the traditional technique, the method provided by the invention greatly shortens the technical process, reduces the production cost, and has obvious wall breaking effect and wide application prospects.

A process for breaking the wall of ganoderma spora with high wall-breaking rate (more than 99%) includes such steps as choosing pearl particles with different diameters from 2 mm to 5 mm, proportionally mixing them with ganoderma spora and water, ball grinding, separating pearl particles from wall-broken spora, drying and pulverizing.

The invention discloses a method for preparing biological grease in algae cell. The method comprises the following steps of: firstly, crushing the algae cell, collecting mixed liquor of crushed algae cell, performing centrifugation, taking supernatant, performing first-time liquid-liquid extraction by using a normal hexane / ethyl acetate mixed organic solvent, and collecting aqueous phase and organic phase; secondly, performing second-time liquid-liquid extraction on the aqueous phase obtained by the first-time liquid-liquid extraction by using the normal hexane / ethyl acetate mixed organic solvent, and collecting aqueous phase and organic phase; thirdly, performing third-time liquid-liquid extraction on the aqueous phase obtained by the second-time liquid-liquid extraction by using the normal hexane / ethyl acetate mixed organic solvent, and collecting aqueous phase and organic phase; fourthly, combining the organic phases obtained by the three-time extraction, and performing vacuum evaporation to obtain the biological grease in algae cell; and finally adding a KOH-containing methanol solution into the biological grease in algae cell, stirring violently, and performing esterification reaction to obtain biodiesel which is in line with 'GB / T 20828-2007 Bildiesel Blend Stock (BD100) for Diesel Engine Fuels'.

A method for breaking the wall of plant cell in order to increase the nutritive value of plant includes such steps as washing raw plant, baking until its water content is 15-40%, heating in pressure tank at 50-80 deg.C for 10-40 min, vacuumizing for 1-3 hr, and cooling.

The invention provides a method for treating sludge by utilizing a microbial flocculant and microbial wall breaking. According to the method, residual sludge is subjected to biological modification with a microbial flocculant, the modified sludge is subjected to biological wall breaking with a microbial wall breaking flora and a heavy metal chelating flora, the content of heavy metals is reduced,and sludge is subjected to aerobic fermentation with a sludge fermentation flora and a biomass fermentation flora, such that the sludge is converted into organic humus. No chemical agent is added in the whole process, no energy is input into the sludge in the dehydration process, only a small amount of mechanical power is used during stacking and turning, the sludge is modified, heated and dried by purely utilizing the activity advantage of microorganisms, and finally reduction, harmlessness, stabilization and recycling of the sludge are achieved. According to the invention, a new method for treating and disposing residual sludge and industrial sludge with high organic matter content in municipal sewage plants is opened up, and the method provided by the invention has the advantages of lowequipment investment, simple operation, safety, no pollution, low energy consumption, and good economic and environmental benefits.

The invention provides a wall-breaking method for haematococcus pluvialis, and relates to a breaking method for a freshwater algae cell wall. Algal species plate cultivation is performed; counting is performed on algal liquid through a blood counting chamber, the algal liquid is inoculated into a triangular bottle containing a first-class seed BBM+V culture medium to be cultured, and first-class seed liquid is obtained when algal bodies grow to a logarithm growth period; the first-class seed liquid is taken to be inoculated into a triangular bottle containing a second-class seed BBM+V culture medium to be cultured, the triangular bottle is shaken by hands for twice everyday to prevent the algal bodies from adhering to the bottle wall, and second-class seed liquid is obtained; a fermentation medium is contained in a triangular bottle, the second-class seed liquid is inoculated according to the initial algal cell density of 5*104 cells / ml, the triangular bottle is placed in an illuminating incubator to be cultured and shaken by hands for twice regularly everyday, and three biological repetitions are performed on each set of cells; wall-breaking treatment is performed on the algal bodies by utilizing a high-pressure homogenizing breaking method or a high-speed bead milling method or a manual grinding method, a sample is taken to be used for analysis on parameters such as carotenoid releasing amount and cell breaking rate.

The invention relates to a process method for producing yeast polysaccharide and yeast nucleotide from yeast, which solves the problems of low yield, long period, high equipment investment and high production cost existing in the conventional process for extracting the yeast polysaccharide and the yeast nucleotide. The yeast polysaccharide and the yeast nucleotide are respectively extracted by taking waste beer yeast as a raw material through the steps of high-pressure homogenization and performing of an enzyme method. The process method has the characteristics of high yield and purity of extracted yeast polysaccharide, high yeast nucleotide content, short extraction time, low enzyme consumption, low equipment investment and production cost and suitability for large-scale industrial production.

The invention discloses a high pressure release type biochemical sludge conditioning method, which is characterized in that sludge is poured into a sealed container; then, the pressure inside the container is raised in a pressurization mode; when the pressure is raised to a rated value, pressure release is completed within an extreme time; microbial cells in the sludge realize wall breaking through gas expansion and oscillation impact generated during the pressure release moment, and the sludge conditioning is realized. The subsequent sludge dehydration effect can be better improved; the method has the advantages that the implementation is simple; the operation is convenient; the treatment efficiency is higher; no side effect is generated, and the like.

The invention provides a preparation method of high sulphate group fucoidan polysaccharide, and belongs to the technical field of active polysaccharides. The method solves the problems that in the process of extracting the fucoidan polysaccharide in the prior art, extraction liquid has many impurities; the polysaccharide leaching efficiency is low; the product purity is not high; the polysaccharide decomposing is caused by high temperature, strong acid or strong alkalinity environment in the preparation process; active groups such as sulphate groups are lost, and the like. According to the method, algal is used as a raw material; firstly, the algal is subjected to ultrafine crushing treatment, so that the algal cells are subjected to wall breaking; the polysaccharide leaching rate is increased; the extraction time is reduced; then, a water extraction method is used for extracting the fucoidan polysaccharide; the polysaccharide decomposing and the sulphate group activity loss can be reduced; the natural ingredients and activity of the fucoidan polysaccharide can be greatly remained; finally, the extraction liquid is subjected to protein removal by trichloroacetic acid, sodium alginate impurity removal through ethanol precipitation, and polysaccharide precipitation by ethanol, so that the high-purity and high sulphate group algal polysaccharide is obtained. The purity of the fucoidan polysaccharide prepared by the method can reach 60 to 70 percent; the organic sulfate radical content reaches 20 to 30 percent.

The invention discloses a telescopic efficient wall-breaking food processer. The telescopic efficient wall-breaking food processer comprises a processer body and a top cover, the top cover is installed at the top of the processer body, and an inner cavity is formed in the processer body; the lower side of the inner cavity is provided with a bottom power cavity, a bottom motor assembly is installed in the bottom power cavity, the output end of the bottom motor assembly is connected with a bottom rotating shaft, the bottom rotating shaft extends upwards out of the bottom power cavity and stretches into the inner cavity, and the tail end of the bottom rotating shaft is provided with a bottom knife; the bottom of the processer body is provided with a plurality of supporting legs; one side of the processer body is provided with a side handle, and the side handle comprises a top side edge, a vertical edge and a bottom side edge. Due to the shake-proof design of the supporting legs, noise generated by shake in the use process can be effectively reduced, and the use experience of a user is improved; besides, due to the upper and lower double-knife design and the telescopic design of an upper knife, the wall breaking effect can be well improved, and the wall breaking efficiency of the wall-breaking food processer is greatly improved.

The invention relates to the field of chemical industry, and discloses an extraction method of Cinnamomum camphora anthocyanin. The method comprises the following steps of: cleaning a collected Cinnamomum camphora raw material, removing kernels, carrying out freeze-drying, pulverizing, and screening to obtain Cinnamomum camphora powder; carrying out leaching degreasing treatment on the Cinnamomum camphora powder by using ethyl acetate according to a certain material-liquid ratio to obtain degreased Cinnamomum camphora powder; mixing the degreased Cinnamomum camphora powder and a deep-eutectic solvent according to a certain material-liquid ratio, diluting with water, and carrying out magnetic sufficient oscillation extraction; carrying out ultrahigh-pressure treatment on the obtained solution under ultrahigh-pressure conditions; adding a certain proportion of back-extraction solution into the obtained solution, centrifuging, and taking the supernate to obtain a Cinnamomum camphora anthocyanin crude extract; and eluting the Cinnamomum camphora anthocyanin crude extract with a macroporous resin and a dextrangel to obtain a Cinnamomum camphora anthocyanin refined extract. The degreasing and deep-eutectic solvent repeated extraction are matched with the ultrahigh-pressure treatment to achieve the excellent wall breaking effect, thereby greatly enhancing the extraction efficiency of the Cinnamomum camphora anthocyanin.

The application discloses a method for extracting marine red shrimp astaxanthin from rhodotorula mucilaginosa, which comprises the steps of breaking walls of the rhodotorula mucilaginosa, and adopting an enzymic method to break the walls of the rhodotorula mucilaginosa, wherein wall breaking conditions are as follows: Beta-glucanase with enzyme activity of 10,000U / g and Beta-mannase with the enzyme activity of 60,000U / g with the ratio of 1:1 are added, and respective enzyme adding quantities are 0.005-0.05g / ml, pH is regulated to be 4.5-5.0, and water bath is performed at 50 DEG C to obtain the wall-broken rhodotorula mucilaginosa; digestion with absolute ethyl alcohol; ultrasonic extraction; removing polar impurities; obtaining the product. According to the method, the wall breaking is more through by adopting an enzymic method, and the wall breaking effect is obviously better than that of a chemical method, and structure completeness of marine red shrimp astaxanthin is favorably kept; the method has greater advantage in the aspect of cost, can be used for large-scale extraction and easy for implementation of industrialized production.

RNA (Ribonucleic Acid) is an important genetic substance in a living organism. The RNA extraction is the basis of molecular biology study; high-quality and high-completeness RNA is needed for downstream molecular biological experiments such as cDNA library construction, in vitro inverse transcription, real-time fluorescent quantitative PCR and Nothem hybridization assay. The project has the main content of providing an efficient bacterial cell wall breaking method; the cell breaking is more complete; the RNA dissolution is more facilitated; the high-quality total RNA capable of being directlyused for the downstream molecular biological experiments is obtained. The method has wide applicability. The bacterium belongs to a gram-positive bacterium. Compared with the other methods, the methodhas the advantage that the extracted RNA concentration is improved by 4 to 5 times. The work innovation is shown in aspects that during the bacterium treatment, Tissue Cell-Destroyer DS1000, lysozymeand glass beads are combined, so that the bacterial cell wall can achieve a good crushing effect; through the use of the Tissue Cell-Destroyer DS1000, the RNA extraction effect is greatly improved.

The invention discloses a preparation method of chlorella noodles, belongs to the technical field of food processing, and overcomes the technical defects in the prior art that noodles are single in taste, poor in nutrient and the like. The preparation method disclosed by the invention comprises the following steps: firstly, adding distilled water into chlorella powder to dilute the chlorella powder into a chlorella liquid; then putting the chlorella liquid at 5-30MPa pressure to be homogenized twice to obtain a cracking liquid; then separately adding cellulase and pectinase into the cracking liquid, wherein the adding amounts of cellulase and pectinase is 10-20% by mass of the cracking liquid, adjusting the pH to 8 and performing enzymolysis for 2h to obtain an enzymolysis liquid, adding diatomite into the enzymolysis liquid, and taking the supernatant; and uniformly stirring the supernatant with flour and water and transferring the mixture into a dough maker to dough paste, and pressing the dough to noodles, further curing and air-drying the noodles to obtain the chlorella noodles. Beside relatively high nutrients, the chlorella noodles prepared by the preparation method also plays a role of adjusting flora in the intestinal tract.

The invention aims to provide a safe, energy-saving, efficient and stable sludge freezing dehydration device and a sludge dehydration method. The sludge freezing dehydration device comprises a feedingdevice, a freezing treatment device and a spiral extrusion filter, the freezing treatment device comprises a refrigerator and a crushing device, the spiral extrusion filter comprises a sludge conveying channel, and the sludge conveying channel sequentially comprises a crushing treatment section, a heating treatment section and a filtering treatment section from back to front along a sludge conveying path. According to the sludge freezing dehydration device and the sludge dehydration method, the sludge is frozen and crushed through the freezing treatment device, the cell structure of microorganisms in the sludge is destroyed, water in cells is separated out, the frozen sludge is melted through the screw extrusion filter, then solid-liquid separation is conducted, and the dehydrated sludgewith the low water content is obtained.

The invention relates to a method for preparing activated carbon through sludge fermentation residues. The method comprises the following steps of 1, mixed strong base pretreatment on sludge, whereinthe remaining sludge is taken, the moisture content of the sludge is adjusted to be 95-98%, a mixed solution of potassium hydroxide and sodium hydroxide is added, and the sludge is subjected to mixedstrong base pretreatment after being heated to 90-95 DEG C; 2, sludge and water separation for a fermentation mixture, wherein the remaining sludge after strong base pretreatment in step 1 is subjected to anaerobic fermentation, and when fermentation is finished, agricultural and forest waste is put in the fermentation mixture to serve as a filter aid; 3, preparation of the activated carbon through the fermentation residues, wherein the fermentation residues after sludge and water separation in step 2 are slowly dried under a low-temperature condition, the dried residues are sent into a carbonizing furnace for high-temperature carbonizing, the carbonized residues are sent to an activator for pelleting activation, the activated residues are sent to an oven for drying, and after drying is finished, the sludge-based activated carbon is obtained. By means of the method, the situation that the agricultural and forest waste is left unused and wasted is reduced, and the problem that it is hard to dispose anaerobic fermentation sludge is solved.

The invention discloses a wall breaking method for preparing nanoscale selenium-enriched yeast. The method comprises the following steps: (1) mixing selenium-enriched yeast with water to prepare a selenium-enriched yeast suspension; (2) adding a photocatalyst into the selenium-enriched yeast suspension prepared in the step (1), uniformly mixing, putting into a dark box, stirring, and carrying out light treatment and centrifugal separation to obtain a photocatalytic selenium-enriched yeast suspension; (3) carrying out ball milling on the prepared photocatalytic selenium-enriched yeast suspension to obtain a ball-milled selenium-enriched yeast suspension; (4) carrying out ultrasonic crushing on the prepared ball-milled selenium-enriched yeast suspension; and (5) repeating the operations in the steps (3) and (4) to obtain the nano-scale selenium-enriched yeast. According to the method, the wall breaking rate of the selenium-enriched yeast can reach 80% or above, the nanocrystallization degree exceeds 70%, cell wall polysaccharide and other nutrient substances in the selenium-enriched yeast are fully released, and absorption and utilization of organic selenium by the human body are promoted.

The invention discloses a sludge treatment method. The sludge treatment method comprises: firstly, preparing grinding balls and conveying sludge to be treated to a cooler; then, adjusting the temperature of the sludge to 5 DEG C to 10 DEG C; then conveying the sludge into a refrigerator and adjusting the temperature of the sludge to -10 DEG C to -5 DEG C; then conveying the freezed sludge into a ball mill and carrying out ball milling on the sludge in cooling gas; conveying the sludge, which is subjected to the ball milling, to a centrifugal machine through utilizing a conveying spiral; after centrifuging and separating, removing clear liquid to finish refrigeration, wall breaking and dehydration of the sludge. According to the sludge treatment method, a sludge refrigeration and wall breaking process is disclosed for the first time, sludge cell walls are broken through a refrigeration method and a colloidal structure in the sludge is damaged, so that a moisture distribution condition in the sludge is changed; breaking requirements of releasing needed sludge cells by interstitial water in the sludge cells are met, so that dehydration performance of the sludge is improved, and defects of an existing dehydration process that only free water and one part of the interstitial water can be removed are overcome.

The invention discloses a method for breaking algae cells. The method is characterized by firstly culturing algae species and acquiring algae liquid, then putting the harvested algae liquid into a reaction kettle and stirring the algae liquid uniformly, pumping a mixed gas of ozone and air in a volume ratio of 1:150 and stirring the substances uniformly, adding the pressure to 0.4-0.8MPa and maintaining the pressure for 1-2 seconds, opening an exhaust valve of the reaction kettle to release the pressure to the normal pressure so that the supersaturated gas is released from the algae liquid and repeating the processes of stirring the substances uniformly, pumping the mixed gas of ozone and air, adding pressure, releasing the pressure and releasing the supersaturated gas from the algae liquid for 40-80 cycles, thus completing breaking of the algae cells. The method has the following beneficial effects that the ozone is taken as a medium, acts on the algae liquid through gas-liquid reaction and is uniformly mixed with the algae liquid under the action of a stirrer and then breaks the algae cells; and the method has the characteristics of simple equipment, convenience in operation, good wall breaking effect and easiness in realization of automatic control.