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Wall breaking method for preparing nanoscale selenium-enriched yeast

A selenium-enriched yeast, nano-scale technology, applied in the field of photocatalysis and biology, can solve the problems of high price of reagents, loss of effective components, and poor popularity, and achieve good size uniformity, improve absorption and utilization, and improve destruction. The effect of wall efficiency

Pending Publication Date: 2022-05-17
NANJING YINGTIAN HEALTH MANAGEMENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the population in my country is deficient in selenium, and the average intake of selenium is less than 40 μg / day, which is lower than the recommended intake of the Chinese Nutrition Society (50-250 μg / day)
However, because the yeast wall is relatively thick (0.1-0.3 μm), in order to promote the dissolution of the active ingredients in the yeast cells, it is necessary to break the yeast cell wall. The commonly used cell wall breaking methods include mechanical method, ultrasonic method, pyrolysis method, chemical method Studies have shown that a single wall-breaking method can only break the yeast cell wall, and cannot reach the ultra-fine level. The particle size of the selenium-enriched yeast suspension is large and uneven, which makes digestion The system does not fully absorb it, resulting in the loss of active ingredients, and the cell wall breaking rate is difficult to reach 80%.
From the perspective of development prospects, the biological method of wall breaking has the advantages of low energy consumption and mild conditions, and is technically feasible. It is a wall breaking method worth looking forward to. and not widespread

Method used

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  • Wall breaking method for preparing nanoscale selenium-enriched yeast
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  • Wall breaking method for preparing nanoscale selenium-enriched yeast

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Prepare 50mL of Zn(NO 3 ) 2 solution and NaOH solution, the NaOH solution was mixed with Zn(NO 3 ) 2 Mix the solutions, continue to stir for 20 minutes, transfer to a centrifuge tube and centrifuge for 5 minutes at a speed of 6000 rpm, discard the supernatant, wash the precipitate with distilled water once, discard the supernatant after centrifugation, and disperse the precipitate in 80mL with a concentration of 1mol / L H 2 o 2 In the solution, under the condition of sufficient stirring, heat to 75°C, stir for 1h, centrifuge for 10min at 8000rpm, wash the precipitate once with distilled water and absolute ethanol, and dry at 80°C for 30min to obtain the ZnO precursor. Then it was placed in a crucible, heat-treated at 400° C. for 2 h, cooled to room temperature, and then taken out to obtain photocatalytically excited ZnO powder.

[0044] Weigh 10 g of selenium-enriched yeast dry powder, dissolve it in water, and prepare a selenium-enriched yeast suspension with a mas...

Embodiment 2

[0048] Weigh 10 g of selenium-enriched yeast dry powder, dissolve it in water, and prepare a selenium-enriched yeast suspension with a mass fraction of 10%. Add 200mg of the ZnO powder obtained in Example 1 to the above-mentioned selenium-enriched yeast suspension, stir evenly at 1200r / min, and place it in a dark box. Under continuous stirring at the same speed, the selenium-enriched yeast suspension is illuminated for 40min, and the light source The distance from the liquid surface of the suspension is about 20 cm, and then centrifuged at 800 r / min for 1 min to obtain a photocatalytic selenium-enriched yeast suspension.

[0049] Then pour the selenium-enriched yeast suspension into a ball mill for grinding, use zirconia as the impact ball, the ball-to-material ratio is 3:1, the ball diameter is 2-3cm, the speed is 500rpm, take it out after 1 hour of ball milling, and use an ultrasonic cell disruptor Ultrasonic treatment for 10 minutes, power 300W, repeated ball milling and ul...

Embodiment 3

[0051] Weigh 10 g of selenium-enriched yeast dry powder, dissolve it in water, and prepare a selenium-enriched yeast suspension with a mass fraction of 10%. Add 200 mg of the ZnO powder obtained in Example 1 to the above-mentioned selenium-enriched yeast suspension, stir evenly at 1000 r / min, and place it in a dark box. With constant stirring at the same speed, irradiate the selenium-enriched yeast suspension for 40 minutes. The distance from the liquid surface of the suspension is about 20 cm, and centrifuged at 650 r / min for 1.5 min to obtain a photocatalytic selenium-enriched yeast suspension.

[0052] Then pour the selenium-enriched yeast suspension into a ball mill for grinding, use zirconia as the impact ball, the ball-to-material ratio is 3:1, the ball diameter is 2-3cm, the speed is 500rpm, take it out after 1 hour of ball milling, and use an ultrasonic cell disruptor Ultrasonic treatment for 10 minutes, power 300W, repeated ball milling and ultrasonic treatment, the t...

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Abstract

The invention discloses a wall breaking method for preparing nanoscale selenium-enriched yeast. The method comprises the following steps: (1) mixing selenium-enriched yeast with water to prepare a selenium-enriched yeast suspension; (2) adding a photocatalyst into the selenium-enriched yeast suspension prepared in the step (1), uniformly mixing, putting into a dark box, stirring, and carrying out light treatment and centrifugal separation to obtain a photocatalytic selenium-enriched yeast suspension; (3) carrying out ball milling on the prepared photocatalytic selenium-enriched yeast suspension to obtain a ball-milled selenium-enriched yeast suspension; (4) carrying out ultrasonic crushing on the prepared ball-milled selenium-enriched yeast suspension; and (5) repeating the operations in the steps (3) and (4) to obtain the nano-scale selenium-enriched yeast. According to the method, the wall breaking rate of the selenium-enriched yeast can reach 80% or above, the nanocrystallization degree exceeds 70%, cell wall polysaccharide and other nutrient substances in the selenium-enriched yeast are fully released, and absorption and utilization of organic selenium by the human body are promoted.

Description

technical field [0001] The invention relates to the fields of photocatalysis technology and biotechnology, in particular to a wall-breaking method for preparing nano-scale selenium-enriched yeast. Background technique [0002] The structure and strength of microbial cell walls depend on the composition of the cell walls and the degree of interconnection between them. The main resistance to breaking cells comes from the covalent bonds connecting the network structure of the cell walls. Knowing the composition and structure of the cell wall will help to choose the appropriate breaking method. Yeast is a single-celled eukaryotic microorganism. The thickness of its cell wall is about 0.1-0.3 μm. When the cell ages, the thickness will increase. The main components of the yeast cell wall are glucan (35%-45%), mannan (40%-45%), protein (5%-10%), chitin (1%-2%), lipids (3% to 8%), inorganic salts (1% to 3%), etc. Studies have found that the cell wall structure of yeast is similar...

Claims

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Application Information

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IPC IPC(8): C12N1/06C12R1/645
CPCC12N1/063C12N1/066
Inventor 王绪新徐锦忠
Owner NANJING YINGTIAN HEALTH MANAGEMENT
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