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93 results about "Yeast suspension" patented technology

As yeast cell suspension become more diluted, the number of yeast cells per cm3 that counted using haemocytometer under the microscope decrease. This is because when more distilled water used, the smaller the ratio of yeast suspension to distilled water become.

Method for preparing nucleotide from waste beer yeast

The invention discloses a method for preparing nucleotide from waste beer yeast. The method comprises the following steps: sieving, debittering and deodorizing the waste beer yeast to prepare a yeast suspension, extracting the yeast suspension for a period of time in a stainless steel reactor by virtue of a high-temperature concentrated salt method; after the extraction is completed, rapidly cooling and filtering to obtain supernatant; carrying out ultrafiltration desalting and concentration by virtue of an ultrafiltration device to obtain a concentrated solution, hydrolyzing the concentrated solution with protease, carrying out high-temperature enzyme deactivation and adjusting the acidity so that protein is denatured and precipitated; centrifuging and taking supernatant; purifying the supernatant through an ultrafiltration device, adding a nuclease for hydrolyzing, carrying out enzyme deactivation, purifying and drying through a spray dryer to obtain yeast-nucleotide powder. Compared with the prior art, since the high-temperature concentrated salt extraction method, the proteolysis technology and the ultrafiltration membrane technology are adopted, the method is easily operated and low in cost, the extraction rate and purity of the nucleotide are increased, the yeast-nucleotide powder prepared by the method is high recovery rate and purity, the value-adding capacity of processing of the waste beer yeast is increased and the huge economic benefits are generated.
Owner:成都宏安生物科技有限公司

Method for preparing esterified monascus for brewed wine

The invention relates to a method for preparing esterified monascus for brewed wine. The method is characterized by including the steps that indica rice is washed, soaked, drained, cooked and cooled to 37-42 DEG C; 10-15% by weight of mixed monascus inoculation solution is mixed quantitatively according to the weight of the rice, the mixture is placed in sterilized jute bags after being evenly mixed and placed in a distiller's yeast room; the mixture is poured out and laid on a distiller's yeast bed after 18 hours; the mixture is cooled to 35-37 DEG C and stacked into a distiller's yeast pile, heat preservation is conducted, the temperature is increased to 48-50 DEG C, and then the distiller's yeast pile is formed again; after 30-38 hours, the mixture is taken out, intermittently ventilated, cooled and cultured for 28-34 hours; brewer's yeast suspensions are sprayed three times according to 10-20% of the weight of the monascus rice; the humidity of the distiller's yeast room is controlled to be 75-85% for 46-50 hours, the monascus is output and aired at the seventh day, and the esterified monascus is obtained. By means of the technology, the esterification capacity of the esterified monascus is improved, the esterified monascus preparation period is shortened, and the quality product rate of the esterified monascus is increased. The added esterified monascus has a special aroma enhancement function.
Owner:FUJIAN NORMAL UNIV

Preparation method of magnetic biological microcapsule for processing organic matters and ammonia nitrogen in water

The invention discloses a preparation method of a magnetic biological microcapsule for processing organic matters and ammonia nitrogen in water. The method comprises the following steps: 1, adding Candida yeast living cells into a disinfected YPD culture medium, culturing at 25-28DEG C under 150-200rpm for 5-6h, taking a bacterial strain liquid obtained after a logarithmic phase, carrying out low temperature drying refrigeration, centrifuging, washing, and carrying out suspension extraction by using normal saline to obtain a bacterial strain Candida yeast suspension with the OD value of 1.5-1.8; 2, mixing sodium alginate and Fe3O4 powder, adding the obtained mixture to deionized water according to the addition amount of sodium alginate of 19-21g/L and the addition amount of the Fe3O4 powder of 1.8-2.2g/L, heating until sodium alginate is completely dissolved, and cooling; and 3, uniformly mixing the yeast suspension with the obtained sodium alginate-iron powder solution according to a volume ratio of 1:9-1:11, adding the obtained solution mixture into a calcium chloride solution in a dropwise manner, curing for 30-40min, and filtering to obtain the microcapsule. The magnetic biological microcapsule prepared in the invention can efficiently degrade the organic matters and ammonia nitrogen in water, can maintain the vitality of thalli, separates the thalli from extraneous adverse environment, can be uniformly distributed in water and separated from water, and is stable in the storage and use process.
Owner:上海艾耐基科技股份有限公司

Preparation method of liquid fermentum rubrum for brewing

The present invention relates to a preparation method of liquid fermentum rubrum for brewing. The method comprises: adopting a 12 DEG Bx malt wort, a 14 DEG Bx rice koji juice and a Monascus purpureus Went secondary seed liquid as first feeding fermentation main raw materials to ferment for 24 h, and then adding a 14 DEG Bx rice koji juice, an Aspergillus niger spore suspension and a yeast suspension to carry out first material supplement fermentation and second material supplement fermentation so as to obtain the liquid fermentum rubrum for mature brewing. According to the present invention, the liquid fermentum rubrum used for the mature brewing and obtained through the method is a thick red liquid; the microscopic examination results show that the liquid fermentum rubrum has the Monascus purpureus Went, the Aspergillus niger hypha is robust, more than or equal to 10<5> of the yeast cells exist in each visual field, and almost no miscellaneous bacteria exists; the liquid fermentum rubrum for the brewing has characteristics of high liquefying ability, high saccharification ability and high fermentation ability; and after the normal mature liquid brewing fermentum rubrum is cultured, the acidity is 2.2-3.2 g/L, and the fermented alcohol odor and mould odor compound odor is provided.
Owner:福建屏湖红生物科技有限公司

Preparation of steamed buns by fermentation method, and evaluation methods for qualities of steamed buns and flour

The invention discloses a preparation of steamed buns by a fermentation method, and evaluation methods for qualities of the steamed buns and flour. A method for preparing the steamed buns comprises the steps of 1) adding 50 mL of a yeast suspension with a mass concentration of yeast being 5-10% in per 200 g of flour, and then adding water until water absorption of the flour is 65%-80%; 2) fermenting obtained dough for 60 min under a condition with a temperature of 37 DEG C and a relative humidity of 80%, loading the fermented dough on a doughsheeter to roll the dough, kneading the dough round by hands, shaping the dough, fermenting the shaped dough for 15 min under a condition with a temperature of 40 DEG C and a relative humidity of 85%, steaming the dough for 20 min with a big fire, taking the steamed dough out, and cooling for 1 h at a room temperature. On the basis, the evaluation methods for the qualities of the steamed buns and the flour are obtained by grading specific volume, shape, outer color and palatability of the steamed buns. The evaluation methods are few in indexes, strong in practicability and good in repeatability, and have important practice guiding significance for controlling the qualities of raw materials and products in industrial production of the steamed buns in China.
Owner:INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI

Preparation method of white spirit red yeast for brewing red yeast white spirit

The invention relates to a preparation method of white spirit red yeast for brewing red yeast white spirit. The preparation method is characterized by comprising the following steps of: soaking and cleaning long-shaped rice with water, and then putting the cleaned long-shaped rice to a steamer to be steamed; adding yeast seed slurry to the steamed rice; putting the yeast material into gunny bags, and putting the gunny bags into a sterilized yeast room to carry out stacking culture; pouring the yeast material out, and reducing the product temperature to 33-35 DEG C; stacking the yeast material; putting the yeast material of which the temperature rises through stacking into gunny bags, and soaking the gunny bags into a water pool to be soaked; blending a mature Aspergillus niger seed solution to the yeast material, and carrying out stacking fermentation; spreading the yeast material for heat dissipation, spraying a little amount of yeast suspension, then stacking the yeast material, covering the stacked yeast with gunny bags to ferment, stirring at intervals, and spraying a little amount of yeast suspension at intervals, wherein the product temperature is controlled between 30 DEG C and 32 DEG C; carrying out stacking fermentation for 5-6 days; and drying the white spirit red yeast which is mature through culturing by using return air with the temperature of 40 DEG C for later use. According to the white spirit red yeast prepared by using the preparation method, through measurement, the saccharifying enzyme activity is 800-1000U / mL, and the fermentation speed and the acidity are low.
Owner:FUJIAN NORMAL UNIV

Method for fermenting vinegar by adding starter propagation Chinese herbal medicine

The invention provides a method for fermenting a vinegar by adding a starter propagation Chinese herbal medicine. The method comprises the following steps: (1) preparing a rhizoma polygonati extracting solution and red-knees herb extracting solution; (2) washing and soaking rice, performing filtration, cooking rice to be free from white cores, spreading for cooling, adding a koji mould spore suspension and the rhizoma polygonati extracting solution, performing stacking cultivation, and then performing ventilating cultivation to obtain rhizoma polygonati koji; (3) activating a brewer's yeast; (4) washing and soaking sticky rice, performing filtration, cooking sticky rice to be free from white cores, adding the rhizoma polygonati koji and an activated brewer's yeast suspension, performing fermentation till the alcoholic degree is stable, performing filtration, and performing wine boiling to obtain a fermented wine; and (5) adding the red-knees herb extracting solution into the fermentedwine, adding water to dilute the fermented wine, performing pasteurization, performing cooling, then adding an activated acetic bacteria suspension, charging into a fermentation tank, performing fermentation under stirring and ventilating conditions till the acidity no longer rises, performing filtration, and performing blending and disinfection to obtain the starter propagation Chinese herbal medicine. By adoption of the method, the vinegar fermentation production efficiency and the flavor of the vinegar are improved, and the health value of the vinegar is also improved.
Owner:SICHUAN UNIV

Method for preparing chicken flavoring from waste beer yeast

The invention provides a method for preparing a chicken flavoring from waste beer yeast. The method comprises the following steps: adding water to the waste beer yeast, uniformly stirring the water and the waste beer yeast, sieving the obtained mixture, centrifuging the sieved mixture, centrifuging and washing the centrifuged mixture, and collecting yeast mud obtained after the centrifugation; placing the yeast mud in a container, adding water, uniformly stirring the yeast mud and the water to prepare a yeast suspension, adjusting the pH value, adding flavor protease, and carrying out enzymatic hydrolysis; deactivating enzymes after the enzymatic hydrolysis ends, centrifuging obtained product, and collecting obtained supernatant to obtain a yeast proteolysis liquid; and adding reducing sugar, thiamine, sulfur-containing amino acids, glycine and chicken fat, adding the obtained material to a high pressure reaction kettle, carrying out a Maillard reaction, introducing condensed water after the reaction ends in order to cool the obtained reaction product to room temperature, and taking out the product to obtain the chicken flavoring. The chicken flavoring produced in the invention has delicious taste, attractive flavor and rich nutrients; and the waste beer yeast is adopted as a raw material, so the production cost is reduced, the environment is protected, and resources are effectively and comprehensively used.
Owner:李宇嘉

Biocontrol yeast active lyophilized powder and preparation method thereof

The invention relates to biocontrol yeast active lyophilized powder and a preparation method thereof, which belongs to the technical field of biological prevention. The lyophilized powder in the invention comprises pichia guilliermondii Y35-1, trehalose and glutathione. The preparation method comprises the following steps: firstly inoculating a NYDB seed culture medium with activated pichia guilliermondii, culturing the pichia guilliermondii, then inoculating a NYTB fermentation culture medium with a seed solution, and fermenting the seed solution; centrifuging a yeast fermentation solution, washing by using sterile water, washing the obtained precipitates by using sterile water, and preparing a yeast suspension; and adding trehalose and glutathione as a lyophilized protection agent, uniformly mixing, loading into a flat plate, vacuum freeze drying, and obtaining the lyophilized powder. The biocontrol yeast active lyophilized powder provided by the invention is good in biocontrol effect, the yeast after the lyophilized powder is rehydrated is suspended, and the nectarine fruit disease rate and the fruit disease speckle diameter can be apparently lower than that of a control group and are significantly different from the control group, so that the lyophilized powder preparation can effectively inhibit the anthracnose rot disease of the nectarine fruit after being rehydrated.
Owner:JIANGSU UNIV
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