17 results about "Yeast suspension" patented technology

The invention discloses a processing method of pigeon blood tofu. The content of protein peptide and the absorbable utilization degree of protein in a pigeon blood tofu finished product are improved. The method comprises the following steps: (1) anticoagulant treatment: adding an anticoagulant into fresh pigeon blood, and uniformly mixing; (2) extracting the pigeon blood protein: adjusting the pH value of the anticoagulant pigeon blood to the isoelectric point of the pigeon blood protein, centrifuging, and collecting the precipitate to obtain the pigeon blood protein; (3) pre-digestion treatment: carrying out enzymolysis pre-digestion treatment on the pigeon blood protein by using pepsin and trypsin in sequence; (4) making pigeon blood bean curds: adding guar gum, carrageenan, calcium chloride and water into the pre-digested pigeon blood protein, performing reflux treatment by using a colloid mill, and then heating gel to obtain the pigeon blood bean curds; (5) inoculating and fermenting: inoculating debaryomyces hansenii suspension into the pigeon blood tofu, and fermenting; and sterilizing the fermented pigeon blood tofu with steam, and cooling to obtain the pigeon blood tofu product.

The application discloses a highland barley yellow rice wine fermenting agent and a preparation method and application thereof, and relates to the technical field of fermentation and brewing of wine. The highland barley yellow rice wine fermenting agent is configured by microbial suspensions of the following types: mold suspensions, yeast suspensions, non-brewing yeast suspensions and lactic acid bacterial suspensions. The highland barley yellow rice wine is prepared by using the highland barley yellow rice wine fermenting agent for fermentation. The fermenting agent is prepared by mixing multiple strains, is used for highland barley yellow rice wine fermentation, can effectively promote the yellow rice wine fermentation process, improves the brewing efficiency, and meanwhile improves the flavor and quality of the product highland barley yellow rice wine.

The invention relates to a method for preparing dried Schizosaccharomyces, preferably S. pombe, yeast product, which product is an Active Dried Yeast (ADY), the method comprising - growing a viable Schizosaccharomyces, preferably S. pombe, in a culture medium, thereby obtaining a grown viable Schizosaccharomyces, preferably S. pombe, yeast having a protein content of 49.0 wt.% or less based on dry yeast weight; - recovering the grown Schizosaccharomyces, preferably S. pombe, yeast from the culture medium and obtaining a suspension of recovered viable Schizosaccharomyces, preferably S. pombe, cells; - drying the suspension of recovered Schizosaccharomyces, preferably S. pombe,, thereby obtaining the Active Dried Yeast, the Active Dried Yeast containing at least 1.0 x 1010 viable Schizosaccharomyces, preferably S. pombe, cells per gram Active Dried Yeast, and having a protein content of 49.0 wt.% or less based on dry weight of the Active Dried Yeast. The invention further relates to Schizosaccharomyces, preferably S. pombe, ADY and various uses thereof.

The invention discloses a making method of manyprickle acanthopanax fruit polysaccharide steamed buns. The preparation method comprises the following specific steps: 1) weighing raw materials: weighing the required raw materials in proportion; 2) raw material pretreatment: sieving the acanthopanax fruit polysaccharide dry powder; 3) polysaccharide extraction: extracting acanthopanax fruit polysaccharide by adopting an ultrasonic water extraction method, and setting ultrasonic parameters: 35 minutes at 55 DEG C, 200W and a material-liquid ratio of 1: 10g / mL. (4) kneading dough: stirring the high gluten flour, the acanthopanax fruit polysaccharide powder, the white granulated sugar and the yeast suspension together, and kneading into dough; 5) fermentation: putting the dough into a constant-temperature fermentation box for fermentation for 60 minutes at the temperature of 40 DEG C and the humidity of 80% RH; (6) shaping and fermenting: kneading the dough until the surface is smooth, and fermenting at room temperature for 20 minutes; and (7) steaming: steaming for 10 minutes at the power of 2100W and then steaming for 10 minutes at the power of 1600W, stopping fire for 5 minutes, and boiling to obtain a finished product. The manyprickle acanthopanax fruit polysaccharide steamed bun takes the flour and the manyprickle acanthopanax fruit polysaccharide as main raw materials, and the prepared manyprickle acanthopanax fruit polysaccharide steamed bun is rich in fragrance, attractive in color and rich in nutrition and can be eaten as functional food.

The invention discloses a making method of purple perilla steamed buns. The preparation method comprises the following specific steps: 1) weighing raw materials: accurately weighing the required raw materials in proportion; 2) raw material pretreatment: sieving the freeze-dried purple perilla powder; 3) yeast activation: adding dry yeast into warm water, and stirring to activate the yeast; (4) kneading dough: stirring the high gluten flour, the purple perilla freeze-dried powder, the white granulated sugar and the yeast suspension liquid together until the materials are completely fused, and kneading into dough; 5) fermentation: putting the dough into a constant-temperature fermentation box for fermentation, and setting parameters as follows: the temperature is 40 DEG C, the humidity is 80% RH, and the fermentation time is 60 min; (6) shaping and fermenting: kneading the dough until the surface is smooth, putting the dough on a steaming grate, and fermenting at room temperature for 20 minutes; and 7) steaming: weighing 2000mL of water, pouring into a stainless steel steamer, steaming for 10 minutes at the power of 2100W, then steaming for 10 minutes at the power of 1600W, stopping fire for 5 minutes, and boiling to obtain the finished product. According to the purple perilla steamed bun disclosed by the invention, the flour and the purple perilla powder are used as main raw materials, and the prepared purple perilla steamed bun is rich in fragrance, attractive in color and rich in nutrition, can be used as staple food and also can be used as health-care food.

The present invention discloses the use of tryptophan in improving the efficacy of yeast in preventing and controlling post-harvest diseases of fruits and vegetables. The invention is characterized in that the yeast is Scheffersomyces spartinae W9, which has a preservation number of CGMCC No. 27149, and the post-harvest diseases of fruits and vegetables are strawberry gray mold and peach brown rot. The invention can further prepare a preparation for improving the efficacy of yeast in preventing and controlling post-harvest diseases of fruits and vegetables. The preparation is characterized in that the yeast is a yeast having a concentration of 1×10 8 cells / mL of yeast suspension is added with tryptophan to a final concentration of 25 μM. The preparation is useful for preparing inhibitors of Botrytis cinerea and / or Sclerotinia sclerotiorum, and for preparing inhibitors of strawberry gray mold and / or peach brown rot. The advantage is that after storage at shelf temperature for three days, the incidence of gray mold in strawberries is reduced by 100%, and the incidence of brown rot in peaches is reduced by 56%.

The invention discloses a Lactobacillus plantarum strain capable of degrading low-molecular aldehydes, a combination thereof, and application of the strain in degrading the fishy smell of duck liver. The strain is characterized in that the strain is an LPC01 strain with a deposit number of CGMCC No. 32002, and the addition amount of the Lactobacillus plantarum suspension to duck liver pâté is 0.5-1.5vt%. The invention further provides an application of the Lactobacillus plantarum combination consisting of a Lactobacillus plantarum suspension and a yeast suspension in equal volumes in degrading the fishy smell of duck liver. The combination comprises the steps of inoculating the yeast suspension into the duck liver pâté for fermentation, and then inoculating the Lactobacillus plantarum suspension for fermentation. The invention has the advantages that, during the fermentation process of the duck liver, the total aldehyde degradation rate of the strain is 63.8%, and under the condition of co-culturing with the yeast, the degradation rate is increased to 97.8%, showing a significant fishy smell removal effect.

The invention discloses a preparation method of nanoscale wall-broken selenium-enriched yeast, and relates to the technical field of food biologication.The preparation method comprises the following steps that selenium-enriched yeast suspension liquid is prepared; the selenium-enriched yeast suspension is subjected to mild local pore pretreatment, and local pores are formed in cell walls; a mixed solution containing amphiphilic molecules is added into the treated suspension liquid, a reaction is carried out under the low-strength shearing condition to form nanosheets, and the amphiphilic molecules comprise beta-cyclodextrin or a derivative thereof; and carrying out stabilizing treatment on the obtained product, and drying to obtain the nano-scale wall-broken selenium-enriched yeast. According to the invention, the nano-scale wall breaking with relatively ordered structure of the cell wall is realized, and the release and absorption efficiency of the selenium component in the gastrointestinal tract can be obviously improved; the wall breaking process is mild, low in temperature and low in shearing, the structural integrity and biological activity of selenium active ingredients are effectively protected, and the retention rate of the active ingredients is remarkably superior to that of a traditional violent wall breaking method.

To provide a method for mass-producing novel yeast-derived microparticles at once without using special apparatus or reagents.SOLUTION: The method includes: at least one of a step (A1) of heating a yeast suspension in which yeast is suspended in an aqueous solvent and a step (A2) of suspending the heated yeast in an aqueous solvent to prepare a yeast suspension; a step (B) of fractionating the yeast suspension into a precipitate fraction and a floating fraction; and a step (C) of concentrating the floating fraction to prepare a floating concentrate.SELECTED DRAWING: Figure 1

The invention belongs to the technical field of biological prevention and treatment, and relates to application of volatile compounds generated based on Caribike Yarrowia pastoris to prevention and treatment of postharvest penicilliosis of pear fruits. The method comprises the following steps: firstly, preparing a yeast suspension, then injecting a PDA culture medium into a sterile sealing box, inoculating the yeast suspension after solidification, and uniformly smearing to obtain a sterile sealing box A; wherein the VOCs comprise 2-phenethyl alcohol; placing a sterilized filter paper sheet completely covering the bottom in the sterile sealing box, and dropwise adding 2-phenethyl alcohol to obtain a sterile sealing box B; the pears in a natural state are placed in the sterile sealing box A or B, and the prevention and treatment of the penicilliosis of the picked pears by the VOCs can be realized. According to the method, the postharvest penicilliosis of the pears is creatively controlled on the basis of VOCs generated by the Caribik Yarrowia, phenethyl alcohol is introduced as a characteristic volatile matter through component analysis, prevention and treatment of the postharvest penicilliosis of the pears are effectively achieved, the substantive technical effect is achieved, and remarkable economic benefits and social benefits are achieved.

The invention belongs to the field of biological control of postharvest diseases of fruits and vegetables, and particularly relates to a preparation method and application of a sodium alginate-gellan gum based antagonistic yeast bioactive membrane. The preservation number of the candida linangbi is CGMCC (China General Microbiological Culture Collection Center) No.33800 Firstly, a yeast suspension is prepared; then preparing a basic membrane solution from sodium alginate, gellan gum, glycerol and water, and adding the yeast suspension to obtain a bacterial-containing membrane solution; and finally, the fruits and vegetables are sequentially immersed in a mycoderm-containing solution and a calcium chloride solution, and after the fruits and vegetables are taken out and aired, a stable sodium alginate-gellan gum base antagonistic yeast bioactive membrane can be formed on the surfaces of the fruits and vegetables, so that the antibacterial and fresh-keeping purposes of the fruits and vegetables are achieved. The candida linangbi has originality, and meanwhile, the antagonistic yeast is encapsulated by adopting an edible coating film, so that the candida linangbi is safe and environment-friendly, the survival rate of the yeast in the film is high, long-acting release of bacteriostatic activity is realized, the shelf life is prolonged, the edible safety is guaranteed, and the candida linangbi has a wide market application prospect.

The invention belongs to the technical field of biological prevention and treatment of postharvest diseases of fruits, and particularly relates to a method for preventing and treating postharvest diseases of grapes, storing and preserving grapes through cyclic adenylic acid induced culture of sporidiobolus pteropteroides Y16. The method comprises the following steps: carrying out induced culture on sporidiobolus roseus Y16 by using an NYDB culture medium containing cAMP with the final concentration of 0.05-0.5 mM, centrifuging to obtain thalli, carrying out resuspension on sterile normal saline to obtain a yeast suspension, punching grapes, injecting the yeast suspension, standing, and injecting an equal-volume aspergillus niger spore suspension to effectively control the sour rot, or uniformly spraying the yeast suspension subjected to induced culture on the surfaces of the grapes, and naturally airing, so as to realize the purposes of disease control, storage and fresh-keeping after the grapes are picked. The method can significantly reduce the occurrence of sour rot after the grapes are picked, reduces the natural rotting rate after the grapes are picked, has no significant adverse effect on main quality indexes of the picked grapes, is safe and environment-friendly, and has a wide market application prospect.

FIELD: food industry.SUBSTANCE: method for producing pan bread from composite wheat-flax-camelina flour is characterized by the fact that the composite wheat-flax-camelina flour is obtained by mixing premium wheat baking flour with flax-camelina flour, taken in the ratio of 95:5, 90:10, 85:15. The content of flaxseed and camelina flour in the composition is taken in equal proportions of 2.5:2.5, 5.0:5.0 and 7.5:7.5, respectively. Next, a yeast suspension is prepared from pressed yeast and a salt solution. Then the dough is kneaded, whereas flour, yeast suspension, salt solution, and the remaining water are fed into the dough mixing machine, the dough is kneaded for 3 minutes, the temperature of the dough after kneading is 30 °C. Then the dough is placed in a proofing cabinet for fermentation for 170 minutes at a temperature of 40 °C and relative humidity of 75-85% with two kneadings after 60 and 120 minutes from the start of fermentation. Next, the dough piece is cut and shaped, then the dough piece is placed on a baking sheet and placed in a proofing cabinet for final proofing for 30 minutes at a temperature of 40 °C and relative humidity 75-85%. Next, the dough piece is baked in a convection oven with humidification of the baking chamber at a temperature of 220 °C for 30 minutes. Then the shaped bread made from composite wheat-flax-camelina flour is cooled at room temperature.EFFECT: increased nutritional and biological value of bakery products.1 cl, 4 tbl

The invention discloses a preparation process of a yeast extract, and particularly relates to a preparation process of a yeast extract. The invention relates to a preparation process of a yeast extract, which comprises the following steps: adding water into washed and impurity-removed clean yeast paste to dilute into yeast suspension, carrying out settling separation on the yeast suspension, pouring out supernate, taking out the supernate obtained in the step S1, adding a debitterizing agent into the supernate to carry out debitterizing and deodorizing treatment, and centrifuging for 10 minutes by using a 2000r / min centrifugal machine to obtain yeast supernate with higher purity, then adding an autolysis accelerant NaCl into the yeast liquid, controlling the temperature, time and pH value of the yeast liquid, enabling the yeast to be autolyzed, preparing an autolysis product, and centrifugally separating an enzymolysis product obtained after enzyme deactivation of the autolysis product, so that the amount of obtained yeast extract clear liquid is large, the amount of dried powdery yeast extract is large, and the extraction rate of the yeast extract is high; the prepared yeast cell wall by-product has various physiological functions of enhancing immunity, preventing and treating diseases, promoting growth, supplementing nutrition and the like, and has economic value.

The invention relates to the technical field of biology, and particularly discloses a preparation method and application of nanoscale selenium-enriched yeast. According to the preparation method, photocatalysis, ball milling, ultrasound and other technical means are integrated in the same device for the first time, and the preparation method comprises the following specific steps: preparing a yeast suspension, carrying out ultrasonic ball milling treatment with photocatalysis, and repeating the ultrasonic ball milling treatment with photocatalysis for 2-3 times, the step of separately operating photocatalysis, ball milling and ultrasound is omitted, multiple times of sample transfer and raw material loss caused in the transfer process are avoided, respective advantages of the three technical means are fully played, the technical effect of synergistic interaction is achieved, the production period is effectively shortened, and the production cost is reduced. And by matching with a specific process and parameter limitation, the damage of nutrient substances is effectively reduced, so that the nano-scale selenium-enriched yeast with high wall breaking rate, high nanocrystallization degree and high nutrient content is obtained.

Method for performing a fermenting process and Apparatus for performing said method, wherein the method for performing a fermenting process in a fermentation vessel, comprising: supplying a yeast suspension into the fermentation vessel (S1), fermenting, during the fermentation process, the yeast suspension within the fermentation vessel (S2), wherein, during the fermenting process, yeast can accumulate in a lower portion of the vessel, and, during at least part of the fermentation process, carrying out a recirculating process in an at least quasi- continuous or continuous manner (S3), which recirculating process comprises: withdrawing yeast suspension, including accumulated yeast, from the lower portion of the vessel (S31), separating the withdrawn yeast suspension by means of a separating device to thereby obtain, from the withdrawn yeast suspension, extracted yeast as retentate and remaining liquid as permeate (S32), and recirculating the permeate into the fermentation vessel (S33).

The present application relates to the technical field of microorganism separation, in particular to a yeast cell separation method and system based on microspotting equipment, comprising: diluting yeast suspension and adding hydrogel to obtain pretreated suspension, and measuring 1 microliter and placing in a microplate; using a microspotting needle to capture a single yeast cell from the 1 microliter suspension and release to a target well, with adsorption negative pressure controlled to 0.02 MPa, slow pressure release when releasing, preheating before adsorption of the spotting needle, and cooling after capturing to maintain cell activity; performing microscopic observation on the receiving well, marking the well containing only a single cell, and adding culture medium for monoclonal culture. The present application realizes controllable separation of a single yeast cell in a 1 microliter limit microvolume, ensures low cell damage through cooperative limitation of mechanical and thermodynamic parameters, eliminates multi-cell mixing through a closed-loop design of spatial separation by hydrogel and end-point verification, and makes conventional microspotting equipment have a single cell sorting function, which is suitable for microbiology research and biomedical development fields.