74 results about "Cell disruption" patented technology

The invention belongs to the field of gene engineering and enzyme engineering, and particularly relates to a method for producing rebaudioside M by an enzyme method. The method comprises the following steps: S1, adding a mixed solution 1 of recombinant cell disruption liquid into a substrate, adding into a buffer solution to obtain a pre-reaction solution 1 (a mixture of recombinant cell disruption liquid of a recombinant strain UGT11 (S158F) and a recombinant strain AtSUS (P94N)), and collecting precipitate after reaction; and S2, after the precipitate is dissolved, adding a mixed solution 2 (a mixture of recombinant cell disruption solutions of a recombinant strain UGT76G1 (G83Q) and a recombinant strain AtSUS (P94N)) of recombinant cell disruption solutions, adding a buffer solution to obtain a pre-reaction solution 2, and collecting the reaction solution to obtain Reb M. The method provided by the invention can effectively solve the problems of low content and poor specificity of the extracted Reb M, and can realize the conversion rate of 93.33% from Reb A to Reb M.

The utility model discloses a kind of cell crushing devices for biological gene detection, it is related to cell crushing technical field, including crushing box, the front surface of crushing box is equipped with box door, hinged joint is connected between the box door and crushing box, the bottom of crushing box is fixedly connected with multiple supporting feet, and the top of crushing box is embedded with operating panel, the middle position of box door is embedded with visual window, the inside of crushing box is fixedly connected with ultrasonic generator near top position, the bottom of ultrasonic generator is connected with conducting rod, the inner surface of crushing box is connected with auxiliary positioning structure.The utility model said a kind of cell crushing devices for biological gene detection, can be positioned to the position of beaker loaded with cell liquid, can be adjusted to the appropriate position, can ensure the accuracy of cell crushing position, can be conveniently supported to the placement seat after adjustment, can improve the relative stability of cell crushing device work.

Methods and kits are provided for producing Griffithsin. The methods include providing a genetically modified microorganism comprising a gene encoding Griffithsin protein operably linked to an inducible promotor and growing the genetically modified microorganism under conditions that induce the promotor and cause expression of griffithisin. The Griffithsin is purified by releasing Griffithsin from the microorganism by cellular disruption, performing a precipitation step to remove contaminating protein and nucleic acids, and performing an anion exchange chromatography step.

This invention discloses a method for preparing the multifunctional bioactive peptide Aj-UBL from sea cucumber, relating to the fields of biotechnology and biomedicine. Using the Aj-UBL coding sequence from sea cucumber as a template, the method involves PCR amplification, recombinant expression vector construction, engineered bacterial induction expression, bacterial cell disruption, affinity chromatography purification, and identification to obtain the target peptide possessing triple activities of antibacterial, antiprotozoal, and antitumor activity. This invention employs an optimized coupling process of genetic engineering and affinity chromatography to achieve high-purity, high-quality, and stable product preparation, while being environmentally friendly and free from drug resistance risks. It not only solves the problem of coordinated disease control in sea cucumber farming but also expands the high-value application of sea cucumber resources in the biomedical field, filling the technological gap in the large-scale preparation of multifunctional bioactive peptides from sea cucumber and the biological control of ciliates, possessing significant theoretical and practical application value.

The utility model provides an efficient sludge wall breaking device which comprises a wall breaking box and a steam assembly, a discharge pipe is installed on the side wall of the wall breaking box in a penetrating mode, a feeding pipe is installed at the top of the wall breaking box in a penetrating mode, and the steam assembly comprises a steam generator located on one side of the wall breaking box. By arranging the exhaust assembly and the adsorption assembly, air can be exhausted outwards through the exhaust assembly during use, so that the interior of the wall breaking box is in a negative pressure state, odor in sludge in the wall breaking box is prevented from escaping from the feeding pipe, and the odor exhausted by the exhaust assembly is exhausted after being adsorbed by the adsorption assembly; according to the wall breaking device, damage to bodies of field personnel can be avoided, meanwhile, pollution to the environment can be reduced, redundant steam in the wall breaking box can preheat sludge passing through the feeding pipe in advance through the preheating assembly in the exhaust process of the exhaust assembly, the steam utilization rate is increased, and energy waste is reduced.

The invention discloses an amide hydrolase mutant and an application in production of nicotinic acid, and provides two novel amide hydrolase mutants, which can significantly improve the production efficiency and yield of nicotinic acid, reduce the operation steps of nicotinic acid synthesis, realize one-step reaction synthesis from nicotinamide to nicotinic acid after cell disruption, break the bottleneck of the prior art, and improve the production efficiency and yield of nicotinic acid. The synthesis time is greatly shortened while the production cost of the nicotinic acid is reduced, and the production of the nicotinic acid is promoted to develop towards a more efficient, more economic and more environment-friendly direction.

1. The name of the design product: grinding instrument. 2. The use of the design product: the instrument for tissue homogenization, sample grinding, cell disruption, homogenization, material dispersion, preparation, sample mixing, oscillation disruption. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: perspective view.

1. The name of the design product: multi-sample tissue grinder (2025). 2. The use of the design product: for tissue homogenization, sample grinding, cell disruption, homogenization, material dispersion, preparation, sample mixing, oscillation disruption, etc. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best shows the design points: perspective view 1.

The utility model discloses an ultrasonication heat preservation device, which belongs to the technical field of cell disruption devices and comprises a disruption machine body, an energy converter is arranged at the top of the disruption machine body, an amplitude-change pole penetrating into the disruption machine body is mounted at the bottom of the energy converter, a horizontally arranged partition plate is arranged on the inner wall of the disruption machine body, and the partition plate is arranged on the outer wall of the disruption machine body. A lifting table is arranged on the partition plate, a water containing tank with an opening in the top end is installed on the lifting table, a cover plate is installed at the top of the water containing tank, a cavity structure extending downwards into the water containing tank is arranged in the middle of the cover plate, and a low-temperature cooling circulating pump is installed at the bottom end of the inner wall of the crusher body; the input end and the output end of the low-temperature cooling circulating pump are communicated with the water containing tank through a water inlet pipe and a water outlet pipe respectively. The cavity structure is used for placing the culture cup, and the cavity structure and the culture cup are separately arranged, so that a sample and the culture cup can be taken down together after the cells are broken.

This invention discloses a method for hydroxylation modification of recombinant mussel foot protein and its application. The invention utilizes a whole-cell catalyst with tyrosinase catalytic activity to hydroxylate tyrosine residues in recombinant mussel foot protein. This method eliminates the need for cell disruption and protein purification steps. By immobilizing tyrosinase on the host cell surface through cell surface display, the recombinant mussel foot protein can directly contact the tyrosinase in the extracellular environment and undergo hydroxylation, thereby introducing dopamine groups into the protein to improve its underwater adhesion properties. This method is applicable to the hydroxylation modification of various types of recombinant mussel foot protein and has advantages such as high catalytic efficiency, good stability, simple operation, and low cost, making it valuable for industrial applications.

The present invention relates to a method for preparing polyhydroxyalkanoate nanobeads. The present invention optimizes a process in which physical cell disruption is performed in order to directly recover polyhydroxyalkanoates, produced in the cells of polyhydroxyalkanoate-producing bacteria, in the original form thereof, and the recovered polyhydroxyalkanoates are washed. Using the optimized method for preparing polyhydroxyalkanoates according the present invention has the effect that polyhydroxyalkanoates can be prepared with high purity and high yield without an organic solvent, and polyhydroxyalkanoate nanobeads can be directly obtained without a separate forming process.

This invention relates to the fields of environmental and bioanalytical technology, and particularly to a method for simultaneous pretreatment and detection of bisphenol S and bisphenol AF in biological tissues. The technical solution includes a framework process for simultaneous pretreatment and detection of bisphenol S and bisphenol AF in biological tissues. This invention establishes a dual-solvent secondary extraction system composed of n-hexane and methyl tert-butyl ether, achieving differentiated adaptation to different tissue matrices. By combining the advantages of non-polar and moderately polar solvents, it can simultaneously handle the relatively highly polar bisphenol S and the relatively non-polar bisphenol AF in a single extraction process. Furthermore, this method sets key physical parameters during the extraction process according to the histological characteristics of each organ. For heart and lung tissues, specific ultrasonic power is used to ensure thorough cell disruption; while for liver and spleen with high lipid content, different power and time are used to ensure the full release of lipid-bound target substances.

This invention belongs to the field of microbial fermentation technology, specifically a process for producing EPA using Schizochytrium fungi as raw material. It employs a dual-gene modified strain with MAT gene overexpression and CLF gene precise knockout, which is domesticated under high-sugar oxidative stress to obtain a highly stable strain. Through three-stage temperature-controlled, stepwise dissolved oxygen fermentation, and the combined addition of para-aminobenzoic acid and ascorbic acid, EPA synthesis is enhanced while oxidative loss is reduced. Highly efficient EPA enrichment is achieved through low-temperature ultrasonic cell disruption of wet cells, urea inclusion, two-stage molecular distillation, and tandem purification using immobilized composite adsorption decolorization. This invention significantly improves EPA yield and proportion, reduces oxidative loss, and enhances product purity and stability through the synergistic effect of gene modification, metabolic regulation, and purification processes. The process is highly controllable, suitable for industrial production, and yields EPA products with high purity and good stability, which can be directly used in the food, health product, and pharmaceutical fields.

The utility model discloses a cell disruption device for biological gene detection, and relates to the technical field of cell disruption, the cell disruption device comprises a device box body, a positioning structure and an overturning structure, the bottom end of the interior of the device box body is fixedly connected with two electric push rods, the top ends of the electric push rods are fixedly connected with connecting plates, a mounting block is arranged between the two connecting plates, and the mounting block is fixedly connected with the positioning structure. An ultrasonic breaker is mounted at the bottom end of the mounting block; according to the device, positioning operation on the crushing container is completed through the positioning structure, extrusion and positioning force is applied to the crushing container from the periphery by controlling a plurality of eccentric wheels, and it is guaranteed that the position of the crushing container is stable in the subsequent crushing process, and deviation cannot occur; according to the device, the opening of the crushing container is shielded through the overturning structure, the two baffles are controlled to be overturned to the position over the crushing container, a sealing cover plate is formed by the two baffles, the opening of the crushing container is partitioned, and a sample is effectively prevented from splashing out of the crushing container.

The invention relates to a hepatocyte growth-promoting factor preparation and a preparation method thereof, and belongs to the technical field of medicines. In order to solve the problems of low cell disruption efficiency, large polypeptide activity loss and long freeze-drying period in the existing hepatocyte growth-promoting factor preparation process, the invention provides a preparation method of a hepatocyte growth-promoting factor preparation, which comprises the steps of raw material pretreatment, low-temperature plasma cell disruption, compound protective agent compounding and pulse microwave freeze-drying. According to the method, an argon-oxygen mixed low-temperature plasma crushing technology is adopted, and the cell breakage rate of 99.7% can be achieved only in 10 min; a mannitol + proline + trehalose composite protective agent system is adopted, so that the activity index of the polypeptide is increased by 15.4%, and the validity period of the product is prolonged to 24 months; by adopting the process coupling design of plasma rapid crushing and pulse microwave rapid freeze-drying, the total production period is shortened to 12.5 hours from 30-36 hours of the traditional process, and the production efficiency is improved by more than 60%.

This invention discloses an unsaponifiable extract of olive oil, its preparation method, and its applications. The preparation method includes: S1, raw material pretreatment: after drying and pulverizing olive pulp, antioxidants and a compound enzyme are added for enzymatic hydrolysis, followed by ultrasonic cell disruption; S2, solvent extraction: extraction is performed using a hexane-anhydrous ethanol compound solvent to obtain an extract; S3, multi-stage saponification: the extract undergoes two stages of saponification sequentially, and the organic phase is collected; S4, purification and refining: after dehydration and concentration, the organic phase is dissolved in squalane and filtered to obtain a refined concentrate. This invention achieves efficient and gentle extraction of high-value components such as squalene and phytosterols through low-temperature processing, enzyme-ultrasonic synergistic cell disruption, and gradient saponification. The obtained product can be directly used in cosmetics, exhibiting excellent anti-aging and skin repair effects, and the formulation is extremely simple, gentle, and non-irritating, showing promising application prospects.

This invention discloses a method for preparing phycocyanin and its related applications, relating to the field of active substance extraction technology. Through a specific sequence of membrane filtration, cell disruption, and chromatography, this invention efficiently and selectively separates and purifies high-purity phycocyanin from the complex composition of *E. coli* fermentation broth. Compared to the cumbersome traditional process relying on algal extraction followed by chemical lysis, this method avoids the seasonality and geographical limitations of raw materials, achieving high recovery rates and highly selective enrichment of phycocyanin. The process flow is clear, the conditions are mild, and it is easy to scale up, demonstrating good industrial scalability potential. This invention not only provides a stable, economical, and efficient technical route for the large-scale preparation of phycocyanin but also lays a solid foundation for its in-depth application in high-value-added fields such as food, pharmaceuticals, and biological detection.

The invention relates to the field of tea production, and discloses a special flavor raw material tea processing method, which comprises the following steps: tea garden management, arginine foliage dressing, and red, blue and yellow multi-light-quality intermittent light stress on leaf surfaces; fresh leaves are picked, and tea tree tender shoots are picked after stress treatment and self-adaption before picking; the white tea processing comprises the following steps: carrying out red, blue and yellow dark and cold and hot air intermittent combination and atomization exogenous addition type first withering on fresh leaves, carrying out multifunctional enzyme activity ventilation addition and cold and hot air intermittent variable air combination secondary withering on the leaves withered for the first time, carrying out high-pressure or ultrasonic cell disruption on the leaves withered for the second time, and carrying out drying and drying. And piling and absorbing fragrance of the cell-broken leaves through warm and wet air containing orchid essential oil and plant-extracted amino acid, and drying the piled and absorbed leaves through combination of hot-air quick drying and far-infrared supplementary drying to obtain the flower-fragrance fresh and sweet white tea. The flower fragrance fresh and sweet white tea is prepared from the comprehensive direction of tea garden management, fresh leaf picking and tea leaf processing, and a high-quality white tea product can be stably obtained.

The invention discloses a multi-section programmable high-voltage electric field sludge wall breaking device and method, and the multi-section programmable high-voltage electric field sludge wall breaking device comprises: a pretreatment unit, which at least comprises a feed pump for pretreating to-be-treated sludge, a sludge feed port, and a security filter; the multiple sections of electric field reaction chambers are connected to the pretreatment unit and are used for carrying out wall breaking treatment on the pretreated sludge, and electric fields for carrying out wall breaking treatment in different electric field reaction chambers are different in intensity and are respectively used for carrying out wall breaking on cells with different cell wall intensities in the pretreated sludge; and the electric field driving unit is connected to the electric field reaction chambers and is used for controlling the different electric field reaction chambers to generate electric fields with different electric field intensities. Compared with the prior art, the wall breaking efficiency is high, wall breaking is uniform, electric energy consumption can be reduced, and the maintenance cost is reduced.

The application discloses a mechanical sludge cell wall breaking device, and particularly relates to the technical field of sludge treatment, which comprises a tank body, a processing assembly for sludge treatment is arranged in the tank body; the processing assembly comprises an outer pipe arranged in the tank body, a feeding pipe movably connected to the top end of the outer pipe through a bearing, and the top end and the bottom end of the outer pipe penetrate through the tank body. The inner diameter of the feeding pipe is changed, so that the feeding pipe can transport sludge with different block sizes. Then, the sludge is sheared through two positive screw conical parts and two reverse screw conical parts in the inner pipe. The sludge is repeatedly sheared through the flow guide ring plate on the outer pipe and the baffle ring plate on the rotating sieve barrel. Meanwhile, the sludge is discharged to the inside of the tank body through the material holes by centrifugal force, and the sludge is further broken by the segmentation thorn in the material holes. Therefore, the sludge can be comprehensively treated in multiple directions and multiple levels, and the practicability of the application is improved.

The utility model discloses an ultrasonic wall breaking device for extracting plant active ingredients, and aims to effectively improve the wall breaking uniformity, promote the active ingredients to be fully released and dissolved and improve the extraction efficiency. Therefore, the ultrasonic wall breaking device for extracting the plant active ingredients comprises a working table, a support is arranged on the working table in a horizontally rotating mode, an ultrasonic vibrator is fixedly installed on the support, an electric lifting table and a cleaning tank are arranged on the working table, an extraction tank is arranged on the electric lifting table, and the ultrasonic vibrator is fixedly installed on the cleaning tank. When the support rotates to a first position, the ultrasonic vibrator is located over the extraction tank, the electric lifting platform can drive the extraction tank to move upwards, so that an ultrasonic vibration rod of the ultrasonic vibrator is inserted into the extraction tank, and when the support rotates to a second position, the ultrasonic vibration rod of the ultrasonic vibrator is inserted into the extraction tank. And the ultrasonic vibrator is positioned right above the cleaning tank.

This invention discloses an optimized method for a highly efficient fragmentation process of desert algae with high retention of active ingredients. The invention uses cell disruption rate, chlorophyll retention rate, and polysaccharide retention rate as core evaluation indicators, focusing on key parameters of the high-pressure homogenization fragmentation process (fracture pressure, fragmentation time, and algal liquor mass fraction). Effective ranges for these parameters are screened through single-factor experiments, and a multivariate synergistic regression model is constructed using response surface methodology to accurately predict the fragmentation effect and the retention rate of active ingredients. Experimental verification shows that the model has excellent fit; under optimal process conditions, the desert algae fragmentation rate is ≥91.5%, the chlorophyll retention rate is ≥76.9%, and the polysaccharide retention rate is ≥72.4%, significantly superior to existing ultrasonic fragmentation and enzymatic hydrolysis processes. This invention has advantages such as simple operation, high fragmentation efficiency, minimal loss of chlorophyll and polysaccharides, and high accuracy in predicting results. The fragmented products can be directly used in the development of desert algae fertilizers and other products, providing key technical support for the efficient industrial utilization of desert algae resources.

The utility model belongs to cell preparation technical field, concretely relates to a kind of tissue disrupter for cell preparation, including cell disrupter body, amplitude rod is installed in cell disrupter body inner wall, cell disrupter body inner wall installs lifting platform, lifting platform transverse end surface abuts beaker, silica gel pad that beaker surface abuts limit ring inner wall is bonded, limit ring other side surface is connected with moving rod, moving rod surface is slidably connected in the sliding slot of the top of mounting box, mounting box inner wall surface is connected with limit rod, limit rod other side surface is connected with fixed plate, the utility model, by the mutual cooperation between limit ring, moving rod, mounting box, sliding slot, limit rod, fixed plate, dovetail block, spring and side block, the beaker placed on lifting platform is clamped and limited, to avoid its in the process of being broken by ultrasonic vibration driven displacement, cause the influence cell breakage processing uniformity and security.

The application discloses a preparation method of rabbit round small sac transfer factor, comprising the following steps: 1) mixing water for injection with ground rabbit round small sac, and homogenizing the mixture by a colloid mill to obtain a homogenate; 2) centrifuging the homogenate after cell crushing, performing tangential flow filtration on the harvested supernatant, and collecting the permeate; 3) performing tangential flow filtration on the permeate by using filter membranes with different molecular weight cut-off pore sizes in stages to obtain crude products of rabbit round small sac transfer factor with required molecular weight ranges; 4) mixing at least one crude product with a certain molecular weight range in step 3) to obtain a primary product of rabbit round small sac transfer factor; and 5) adjusting the pH and the osmotic pressure of the primary product of rabbit round small sac transfer factor after virus removal, performing sterilization filtration, and obtaining rabbit round small sac transfer factor. The application adopts the tangential flow filtration in stages, establishes precise and effective separation and concentration technology of rabbit round small sac transfer factor and small molecule purification technology, and can prepare rabbit round small sac transfer factors with various molecular weight ranges according to different technological process flows by selecting filter membranes with different molecular weight cut-off pore sizes.

The present application relates to a method, apparatus and system for inspecting a cell crush, a computer device, a storage medium, and a computer program product. The method may include: acquiring images of a flowing cell assembly by a high-speed image acquisition device; extracting image features from the images of the cell assembly; performing similarity matching between the image features and a preset crush model to obtain a similarity matching result; and obtaining a cell crush inspection result based on the similarity matching result.

A construction method of double immobilized recombinant yeast engineering bacteria, engineering bacteria, a catalyst and an application in synthesis of rebaudioside. In the present invention, yeast is used as the chassis cell, an ordered self-assembled multi-enzyme cascade surface display system is constructed, and carrier-free immobilization is coupled with uridine piphosphate glucose (UDPG) in-situ regeneration technology, resulting in a novel yeast whole cell catalyst with double immobilization of enzymes and bacteria. The catalyst uses cheap steviol glycoside (St) and rebaudioside A (Reb A) as substrates, and can synthesize rebaudioside (Reb D / M) in an efficient and economical one-pot method without additional addition of expensive UDPG. The synthesis process does not require cumbersome operations including cell disruption, enzyme separation, purification and immobilization, and avoids bottleneck problems in the related art including material transmembrane transport resistance, mass transfer resistance and product hydrolysis by intracellular enzyme. The catalyst has reusability, and can realize continuous and high-intensity rebaudioside biosynthesis through strain rejuvenation, which provides strong technical support for its industrial application.

This invention relates to a method for preparing highly active biomass masterbatches using an air separation nitrogen generation coupled with electrolytic hydrogen-rich water biomass extraction device, and its application, belonging to the field of high-value biomass extraction technology. Based on an integrated extraction device, this invention designs a dual-path differentiated extraction process according to the differences in the thermosensitivity of the raw materials: for non-thermally sensitive / highly lignified raw materials, a path of "low-temperature pretreatment with hydrogen-rich water → high-temperature steam explosion cell disruption → rapid nitrogen cooling → low-temperature high-pressure extraction with hydrogen-rich water and nitrogen" is used; for thermosensitive raw materials, a path B of "low-temperature leaching with hydrogen-rich water → low-temperature high-pressure extraction with nitrogen" is used. The entire process achieves self-production of nitrogen and hydrogen-rich water, eliminating the need for external gas and water sources. This invention can scalably prepare various highly active biomass masterbatches such as willow tea thearubigin, whole tea polyphenols / theanine / catechins, corn germ SOD three enzymes, ginsenosides, and bird's nest peptides, with a masterbatch activity retention rate ≥98% and a significantly improved yield; simultaneously, it protects the masterbatch and its applications in food, health products, and cosmetics.

The invention relates to the technical field of bioengineering and protein engineering, in particular to an escherichia coli genetically engineered bacterium and a high-throughput screening method of an enzyme. According to the high-throughput screening method of the enzyme, the enzyme is displayed on the surface of escherichia coli, and the surface display efficiency of the enzyme is remarkably improved by optimizing an escherichia coli curli fimbriae system, so that the screening efficiency of the enzyme is improved, the screening steps are reduced, and the screening cost is reduced. The method solves the problems of need of cell disruption, complex operation, easy reduction of protein activity and low screening efficiency of a traditional screening method in an enzyme directed evolution process, and has the advantages of no need of cell disruption, simple and easy operation, high flux, specific and accurate enzyme activity determination, wide enzyme activity detection range, high screening efficiency and the like; good application prospects are realized.

The present invention relates to a method for producing polyhydroxyalkanoate (PHA) and a method for monitoring cell disruption. The present invention provides a method for producing polyhydroxyalkanoate (PHA) that enables the length and sphericity of cells or PHA to be measured by three-dimensional image analysis utilizing the difference in refractive index of the cells or PHA, thereby identifying the degree of autolysis, supply state, or degree of disruption of PHA cells, thereby enabling optimal disruption conditions to be set and enhancing the stability of the impurity removal process, which is a process subsequent to the disruption process.

The invention discloses a graphene / copper reinforced epoxy near-zero-wear friction material as well as a preparation method and application thereof. The method comprises the following steps: carrying out ball-milling stripping and charge functionalization on graphene by utilizing ionic liquid (ILs), intercalating the ILs into graphene sheet layers, and carrying out cell disruption and centrifugal treatment to obtain stable dispersion liquid; then mixing with epoxy resin to prepare an electrophoretic deposition emulsion, and depositing by taking a copper foil as an anode and carbon steel as a cathode to form a Gr-ILs-EP-coated Cu coating; under the action of an electric field, graphene is directionally arranged, copper ions are co-deposited on a substrate, and the wear resistance is remarkably improved; the prepared coating has a lowest friction coefficient of 0.025 under the conditions of 200rpm and 3N, realizes an almost zero wear state, keeps stable in a 5h friction test, has excellent self-lubricating performance, and has a wide application prospect in the field of instrument surface protective coatings.