122 results about "Cell disruption" patented technology

The invention belongs to the field of gene engineering and enzyme engineering, and particularly relates to a method for producing rebaudioside M by an enzyme method. The method comprises the following steps: S1, adding a mixed solution 1 of recombinant cell disruption liquid into a substrate, adding into a buffer solution to obtain a pre-reaction solution 1 (a mixture of recombinant cell disruption liquid of a recombinant strain UGT11 (S158F) and a recombinant strain AtSUS (P94N)), and collecting precipitate after reaction; and S2, after the precipitate is dissolved, adding a mixed solution 2 (a mixture of recombinant cell disruption solutions of a recombinant strain UGT76G1 (G83Q) and a recombinant strain AtSUS (P94N)) of recombinant cell disruption solutions, adding a buffer solution to obtain a pre-reaction solution 2, and collecting the reaction solution to obtain Reb M. The method provided by the invention can effectively solve the problems of low content and poor specificity of the extracted Reb M, and can realize the conversion rate of 93.33% from Reb A to Reb M.

The present invention provides an aqueous polyhydroxyalkanoate coating, a preparation method therefor, and a use thereof. The preparation method for the aqueous polyhydroxyalkanoate coating comprises: subjecting a polyhydroxyalkanoate fermentation solution to cell wall breaking and extraction, to obtain an aqueous polyhydroxyalkanoate dispersion, and then mixing the aqueous polyhydroxyalkanoate dispersion with an additive to obtain the aqueous polyhydroxyalkanoate coating, a dispersant and talcum powder being added during the cell disruption process. By means of using talcum powder to replace a portion of a dispersant as a protective additive for PHA particles, the present invention can prevent the aggregation of PHA particles at high purity, and the resulting aqueous PHA coating exhibits good coating water resistance.

The invention belongs to the technical field of tea processing, and particularly relates to a high-precision grading and directional processing system for Qihong tea. The system comprises an intelligent tea leaf grading module based on hyperspectral imaging and machine vision, wherein the intelligent tea leaf grading module is used for realizing accurate classification of raw materials; an AI-controlled dynamic withering module is used for optimizing enzyme activity and water evaporation; a pressure-rotating speed self-adaptive intelligent rolling module ensures that cells are uniformly broken; an electronic nose is combined with a multispectral real-time fermentation monitoring module to accurately control the fermentation degree; a far infrared-hot air coupled precise drying module is used for realizing gradient dehydration; the near infrared spectrum and deep learning quality detection module is used for completing finished product quality evaluation; the data traceability module of the block chain technology is used for recording whole-process processing parameters; and a big data-driven process optimization module continuously improves the product stability. According to the invention, the intellectualization, precision and standardization of the Qihong Huang tea processing process are realized, and the consistency and the superior product rate of the product quality are obviously improved.

The invention belongs to the technical field of microorganism application, and particularly relates to a genetically engineered bacterium for producing L-alanine and application of the genetically engineered bacterium in two-stage fermentation of the L-alanine, and the genetically engineered bacterium takes vibrio natrievibrio as a chassis bacterium and can co-express an alanine dehydrogenase gene and an alanine transporter gene. An alanine dehydrogenase gene is heterologously introduced into a vibrio natriticus genome, and an inactivated gene combination with the optimal L-alanine yield is found out by randomly inactivating a key enzyme in a byproduct production pathway, so that the vibrio natriticus genetically engineered bacterium capable of efficiently producing the L-alanine through a fermentation method is constructed. According to the present invention, the L-alanine is produced through the two-stage fermentation by using the gene engineering bacteria through the temperature-sensitive control strategy so as to reduce the influence of the accumulation of the L-alanine on the cell growth, and the high production of the Xiabanmate is provided. The genetically engineered bacterium is short in growth cycle, glucose can be efficiently utilized to convert the genetically engineered bacterium into L-alanine, the L-alanine is transferred to the outside of cells, separation and purification are simple, cell disruption is not needed, and the yield and conversion rate of the L-alanine can both reach a high level.

The invention discloses a traditional Chinese medicine preparation method combining ultrasonic waves and directional enzymolysis, and relates to the technical field of traditional Chinese medicine preparation, and the method comprises the following steps: S1, raw material pretreatment: cleaning and drying traditional Chinese medicinal materials, and crushing the traditional Chinese medicinal materials to 20-100 meshes by using mechanical crushing equipment to obtain crushed materials; s2, ultrasonic pretreatment: mixing the crushed material obtained in the step S1 with water according to a mass-volume ratio of 1: 5 to 1: 8 to form an extraction water bath; an ultrasonic generator is adopted to conduct pretreatment on the extraction water bath, the ultrasonic frequency is 20-40 kHz, the power is 300-800 W, the treatment temperature is 30-60 DEG C, and the treatment time is 10-40 minutes. According to the method, cell structures of the traditional Chinese medicinal materials are efficiently disintegrated through the synergistic effect of cavitation effect physical wall breaking of ultrasonic waves and biological directional enzymolysis of the compound enzyme, so that the extraction rate of target effective components such as astragaloside, total flavonoids and polysaccharides is remarkably increased, meanwhile, dissolution of impurities is reduced due to specificity of enzymolysis reaction, and the quality of the traditional Chinese medicinal materials is improved. Therefore, the traditional Chinese medicine extract with higher purity and better color is obtained.

The utility model discloses a kind of cell crushing devices for biological gene detection, it is related to cell crushing technical field, including crushing box, the front surface of crushing box is equipped with box door, hinged joint is connected between the box door and crushing box, the bottom of crushing box is fixedly connected with multiple supporting feet, and the top of crushing box is embedded with operating panel, the middle position of box door is embedded with visual window, the inside of crushing box is fixedly connected with ultrasonic generator near top position, the bottom of ultrasonic generator is connected with conducting rod, the inner surface of crushing box is connected with auxiliary positioning structure.The utility model said a kind of cell crushing devices for biological gene detection, can be positioned to the position of beaker loaded with cell liquid, can be adjusted to the appropriate position, can ensure the accuracy of cell crushing position, can be conveniently supported to the placement seat after adjustment, can improve the relative stability of cell crushing device work.

The invention provides a method for efficiently preparing active polypeptide by fermenting rapeseed dregs, which comprises the following steps: pretreatment: crushing rapeseed dregs, adding 3-5% of reducing sugar, humidifying and sterilizing; performing mixed bacteria segmented fermentation: inoculating a composite strain consisting of bacillus licheniformis SWJS33 with high yield of aminopeptidase, aspergillus oryzae and lactobacillus plantarum, and fermenting at 30 DEG C or 37 DEG C for 72-84 hours; ultrasonic wall breaking: adding water after fermentation, uniformly mixing, carrying out ultrasonic treatment for 20-30 minutes under the power of 120-200W, and breaking thalli to release intracellular enzyme; enzymolysis: carrying out enzymolysis for 4-6 hours under the condition of 50-60 DEG C; post-treatment: enzyme deactivation, centrifugation and drying are carried out after enzymolysis, and a polypeptide product is obtained. By adding the exogenous reducing sugar, nutrition is enhanced, and thallus proliferation is accelerated; bacillus licheniformis SWJS33 with high yield of aminopeptidase is inoculated, so that the generation of small-molecule active peptide is further promoted; a mixed bacteria composite system and a segmented fermentation technology are adopted, and bacteria cells are crushed in an ultrasonic-assisted manner, so that the enzymolysis efficiency is enhanced, and the yield and activity of the polypeptide are remarkably improved.

Polyhydroxyalkanoate-producing microorganisms are cultured to obtain bacterial cells that accumulate polyhydroxyalkanoate particles and have an average cell diameter of 2 μm or greater. The bacterial cells are then heat-treated to reduce the average particle size of the polyhydroxyalkanoate particles within the bacterial cells to 1.8 μm or greater and the average cell diameter of the particles is not greater. Furthermore, the heat-treated bacterial cells are disrupted, and PHA particles can be separated from the aqueous phase of the resulting cell disrupted liquid.

This invention discloses an inteptide Ssp tau, characterized by its amino acid sequence as shown in SEQ ID No. 2. It also discloses its encoding gene and its application in the preparation of tetrapeptide-5. Furthermore, it discloses the Ssp tau-tetrapeptide-5 recombinant protein, the corresponding encoding gene, the expression vector, and the expression host bacteria. This invention discloses a novel inteptide Ssp tau and an inteptide Ssp tau-mediated tetrapeptide-5 expression and purification technique. Ssp tau and tetrapeptide-5 are combined to form a fusion protein, and the fusion protein gene sequence is constructed into PET-28a(+) to obtain a recombinant expression vector for tetrapeptide-5. Through simple steps such as induced expression, cell disruption, ultrafiltration, inteptide autocleavage, secondary ultrafiltration, and rotary lyophilization, high-purity tetrapeptide-5 can be obtained efficiently. This biosynthetic method is suitable for the industrial production of tetrapeptide-5 and has significant market value.

The utility model discloses a carrier for a cell crusher, the carrier for the cell crusher comprises a base and a carrier main body, the carrier main body is fixedly installed at the top end of the base, the carrier for the cell crusher comprises the base and the carrier main body, the carrier main body is fixedly installed at the top end of the base, and the carrier main body is fixedly installed at the top end of the base. A plurality of sample tube accommodating holes are formed in the carrier frame main body, the plurality of sample tube accommodating holes are arranged in a square matrix, gaps exist among the sample tube accommodating holes, and the sample tube accommodating holes are used for placing sample containers. The carrier for the cell disruption instrument is beneficial to control of temperature in the water tank, can rapidly dissipate heat to prevent sample denaturation caused by ultrasonic heat production in the cell disruption process, and is simple in structure, easy to manufacture, low in use cost, wide in application range and high in practicability.

Methods and kits are provided for producing Griffithsin. The methods include providing a genetically modified microorganism comprising a gene encoding Griffithsin protein operably linked to an inducible promotor and growing the genetically modified microorganism under conditions that induce the promotor and cause expression of griffithisin. The Griffithsin is purified by releasing Griffithsin from the microorganism by cellular disruption, performing a precipitation step to remove contaminating protein and nucleic acids, and performing an anion exchange chromatography step.

This invention discloses a method for preparing the multifunctional bioactive peptide Aj-UBL from sea cucumber, relating to the fields of biotechnology and biomedicine. Using the Aj-UBL coding sequence from sea cucumber as a template, the method involves PCR amplification, recombinant expression vector construction, engineered bacterial induction expression, bacterial cell disruption, affinity chromatography purification, and identification to obtain the target peptide possessing triple activities of antibacterial, antiprotozoal, and antitumor activity. This invention employs an optimized coupling process of genetic engineering and affinity chromatography to achieve high-purity, high-quality, and stable product preparation, while being environmentally friendly and free from drug resistance risks. It not only solves the problem of coordinated disease control in sea cucumber farming but also expands the high-value application of sea cucumber resources in the biomedical field, filling the technological gap in the large-scale preparation of multifunctional bioactive peptides from sea cucumber and the biological control of ciliates, possessing significant theoretical and practical application value.

The invention relates to the field of tobacco, in particular to an expression cassette, an expression vector and a preparation for improving tobacco quality and application thereof. The present invention provides an expression cassette comprising: a gene encoding carotenoid cleavage dioxygenase and a promoter. Probiotic escherichia coli E. coli Nissle 1917 is used as an expression host, genetically engineered bacteria for heterologously expressing carotenoid cleavage dioxygenase are constructed, endotoxin is prevented from being introduced in the tobacco leaf fermentation process, and a biological preparation capable of increasing aroma substances of tobacco leaves can be obtained through simple cell disruption. Meanwhile, the content of aroma substances such as geranyl acetone and ionone in the tobacco leaves can be remarkably increased through tobacco leaf fermentation.

The present invention relates to a recombinant bacterium producing Arctic-derived β-glucosidase and its application, belonging to the field of biotechnology. The target gene of the recombinant bacterium is β-glucosidase derived from Arctic bacteria Arc12, the nucleotide sequence of which is shown in SEQ ID No: 1, and the nucleotide sequence of the β-glucosidase with signal peptide is shown in SEQ ID No: 2, the signal peptide is Bacillus subtilis signal peptide CitH, and the expression vector is Escherichia coli-Bacillus shuttle plasmid pMA5, which contains constitutive promoter P HpaII The expression strain is Bacillus subtilis WB600. The present invention provides a method for secretory expression of the enzyme under specific promoter, signal peptide, and strain conditions. This method is simple to operate and can be achieved without the need for special inducers. Secretory expression of the enzyme eliminates the need for cell disruption in downstream processing, reducing production costs and possessing promising application prospects.

The application discloses a kind of cobalt-based MOF material ethanol dispersion and its preparation method and application in third-order nonlinear optics.The preparation method comprises: making cobalt source and organic ligand react, and prepare cobalt-based MOF material;Wherein, the organic ligand includes 1,3,5-tri (4-carboxyl phenyl acetylene group) benzene;And, the cobalt-based MOF material is mixed with ethanol and is broken by cell crushing device, and prepare cobalt-based MOF material ethanol dispersion;Wherein, the power of the breaking treatment is 30%-50%, and the temperature of condensate water in cell crushing device is controlled to be 1-5 ℃.The cobalt-based MOF material ethanol dispersion prepared by the application has excellent performance and reliability in optical third-order nonlinear characteristics, lays a solid practical foundation for the wide application of future all-optical devices, and is expected to promote the further development of optical technology in communication, sensing, information processing and other fields.

The utility model provides an efficient sludge wall breaking device which comprises a wall breaking box and a steam assembly, a discharge pipe is installed on the side wall of the wall breaking box in a penetrating mode, a feeding pipe is installed at the top of the wall breaking box in a penetrating mode, and the steam assembly comprises a steam generator located on one side of the wall breaking box. By arranging the exhaust assembly and the adsorption assembly, air can be exhausted outwards through the exhaust assembly during use, so that the interior of the wall breaking box is in a negative pressure state, odor in sludge in the wall breaking box is prevented from escaping from the feeding pipe, and the odor exhausted by the exhaust assembly is exhausted after being adsorbed by the adsorption assembly; according to the wall breaking device, damage to bodies of field personnel can be avoided, meanwhile, pollution to the environment can be reduced, redundant steam in the wall breaking box can preheat sludge passing through the feeding pipe in advance through the preheating assembly in the exhaust process of the exhaust assembly, the steam utilization rate is increased, and energy waste is reduced.

The invention provides an ultrasonic cell disruption instrument, and belongs to the technical field of cell disruption, the ultrasonic cell disruption instrument comprises a cell disruption instrument, a working bin is reserved in the cell disruption instrument, an ultrasonic probe is assembled on the cell disruption instrument, a support is assembled in the working bin, the support is assembled on the lower portion of the ultrasonic probe, a containing opening is reserved in the support, and the containing opening is communicated with the working bin. A containing piece is connected to the containing opening in a sliding mode. The problems that when an existing ultrasonic cell disruption instrument disrupts cells, a containing bottle containing cell sap to be disrupted is directly placed under an ultrasonic probe, vibration generated during cell disruption easily causes displacement of the containing bottle, the cell sap is easily deviated from a focus area of the ultrasonic probe, and the cell sap is prone to be disrupted are solved. And the action efficiency of the cavitation effect is reduced.

The present disclosure discloses a method for preparing an Oat Protein Sponge (OPS) filter for feeding bottles to remove microplastics. The method involves isolating proteins from oats using cell disruption with alkali. The oats are cultivated through tissue culture and / or hydroponics. The isolated protein is then made soluble through incubation and breakdown, followed by neutralization with a strong acid such as Hydrochloric or Sulfuric acid. The resulting protein is concentrated oat protein, treated with a crosslinking agent, and formed into a sponge structure. This structure is incubated to create a stable hydrogel, which is subsequently washed and purified. The OPS filter effectively removes microplastics from milk and is integrated into feeding bottles, providing a solution to enhance milk safety and quality for infants.

The invention discloses a method for activating an original phage based on a low-voltage electrochemical technology. The method comprises the following steps: performing low-voltage electrolysis on a bacterial electrolyte containing the original bacteriophage by adopting a double-electrode system, performing electrolytic treatment, culturing a solution after reaction in a culture solution, sequentially performing cell disruption, centrifugal precipitation, supernatant filtration and bacteriophage extraction, and detecting the number of bacteriophages generated after the original bacteriophage is activated by adopting a double-layer plate method. The method has the characteristics of simplicity in operation, low energy consumption and environmental friendliness by efficiently producing the active free radical to activate the prophage.

The invention discloses an amide hydrolase mutant and an application in production of nicotinic acid, and provides two novel amide hydrolase mutants, which can significantly improve the production efficiency and yield of nicotinic acid, reduce the operation steps of nicotinic acid synthesis, realize one-step reaction synthesis from nicotinamide to nicotinic acid after cell disruption, break the bottleneck of the prior art, and improve the production efficiency and yield of nicotinic acid. The synthesis time is greatly shortened while the production cost of the nicotinic acid is reduced, and the production of the nicotinic acid is promoted to develop towards a more efficient, more economic and more environment-friendly direction.

1. The name of the design product: grinding instrument. 2. The use of the design product: the instrument for tissue homogenization, sample grinding, cell disruption, homogenization, material dispersion, preparation, sample mixing, oscillation disruption. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: perspective view.

1. The name of the design product: multi-sample tissue grinder (2025). 2. The use of the design product: for tissue homogenization, sample grinding, cell disruption, homogenization, material dispersion, preparation, sample mixing, oscillation disruption, etc. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best shows the design points: perspective view 1.

The utility model relates to an ultrasonic cell disruption device, which comprises an ultrasonic generator and a soundproof box, a lifting platform for placing a sample container is arranged in the soundproof box, a transducer penetrating from the top of the soundproof box is arranged above the lifting platform, the lower end of the transducer is connected with an amplitude transformer, and the lower part of the amplitude transformer extends into the sample container. The amplitude-change pole is sleeved with a protective sleeve with the lower end closed. The ultrasonic cell disruption device disclosed by the utility model is novel in structure, reasonable in design, convenient and practical, the amplitude-change pole can be sealed and wrapped by adding the protective sleeve on the amplitude-change pole, a sample is prevented from being in direct contact with the amplitude-change pole, a heat source is isolated, the protective sleeve can be reused, and the investment cost is low.

The utility model discloses an ultrasonication heat preservation device, which belongs to the technical field of cell disruption devices and comprises a disruption machine body, an energy converter is arranged at the top of the disruption machine body, an amplitude-change pole penetrating into the disruption machine body is mounted at the bottom of the energy converter, a horizontally arranged partition plate is arranged on the inner wall of the disruption machine body, and the partition plate is arranged on the outer wall of the disruption machine body. A lifting table is arranged on the partition plate, a water containing tank with an opening in the top end is installed on the lifting table, a cover plate is installed at the top of the water containing tank, a cavity structure extending downwards into the water containing tank is arranged in the middle of the cover plate, and a low-temperature cooling circulating pump is installed at the bottom end of the inner wall of the crusher body; the input end and the output end of the low-temperature cooling circulating pump are communicated with the water containing tank through a water inlet pipe and a water outlet pipe respectively. The cavity structure is used for placing the culture cup, and the cavity structure and the culture cup are separately arranged, so that a sample and the culture cup can be taken down together after the cells are broken.

This invention discloses a method for hydroxylation modification of recombinant mussel foot protein and its application. The invention utilizes a whole-cell catalyst with tyrosinase catalytic activity to hydroxylate tyrosine residues in recombinant mussel foot protein. This method eliminates the need for cell disruption and protein purification steps. By immobilizing tyrosinase on the host cell surface through cell surface display, the recombinant mussel foot protein can directly contact the tyrosinase in the extracellular environment and undergo hydroxylation, thereby introducing dopamine groups into the protein to improve its underwater adhesion properties. This method is applicable to the hydroxylation modification of various types of recombinant mussel foot protein and has advantages such as high catalytic efficiency, good stability, simple operation, and low cost, making it valuable for industrial applications.

The present invention relates to a method for preparing polyhydroxyalkanoate nanobeads. The present invention optimizes a process in which physical cell disruption is performed in order to directly recover polyhydroxyalkanoates, produced in the cells of polyhydroxyalkanoate-producing bacteria, in the original form thereof, and the recovered polyhydroxyalkanoates are washed. Using the optimized method for preparing polyhydroxyalkanoates according the present invention has the effect that polyhydroxyalkanoates can be prepared with high purity and high yield without an organic solvent, and polyhydroxyalkanoate nanobeads can be directly obtained without a separate forming process.

This invention relates to the field of downstream product processing technology in bioengineering, specifically disclosing a method for directly extracting polyhydroxyalkanoates (PHA) from fermentation broth using plasticizers. The method includes: first, adding a certain amount of plasticizer to the fermentation broth producing PHA, mixing thoroughly to form a turbid liquid for cell disruption and extraction; then, performing solid-liquid separation to remove the aqueous phase and plasticizer, retaining the PHA-containing solid phase; second, repeatedly washing with detergent to remove residual plasticizer and other impurities, obtaining a solid after solid-liquid separation; finally, drying the solid to obtain the PHA; the plasticizer and detergent used are recycled. This method integrates cell disruption and PHA extraction, featuring a simple process, environmental friendliness, low investment cost, low energy consumption, high production efficiency, high product purity, and stable quality, making it suitable for industrial production.

This invention relates to the fields of environmental and bioanalytical technology, and particularly to a method for simultaneous pretreatment and detection of bisphenol S and bisphenol AF in biological tissues. The technical solution includes a framework process for simultaneous pretreatment and detection of bisphenol S and bisphenol AF in biological tissues. This invention establishes a dual-solvent secondary extraction system composed of n-hexane and methyl tert-butyl ether, achieving differentiated adaptation to different tissue matrices. By combining the advantages of non-polar and moderately polar solvents, it can simultaneously handle the relatively highly polar bisphenol S and the relatively non-polar bisphenol AF in a single extraction process. Furthermore, this method sets key physical parameters during the extraction process according to the histological characteristics of each organ. For heart and lung tissues, specific ultrasonic power is used to ensure thorough cell disruption; while for liver and spleen with high lipid content, different power and time are used to ensure the full release of lipid-bound target substances.

The invention discloses a tea enzyme activation fermentation method which comprises the steps of fresh leaf pretreatment, ultrasonic-assisted low-temperature cold beating, gradient variable-temperature circulating cell breaking, multi-stage enzyme activation fermentation, activity locking storage, secondary drying, quality fine adjustment and finished product grading and packaging. By optimizing a low-temperature treatment process, introducing an ultrasonic-assisted technology and designing a multi-section temperature-humidity coupling regulation system, accurate activation of endogenous enzyme activity of tea and controllable regulation of a fermentation process are realized, so that a tea product which is rich in flavor level, high in freshness and capable of realizing customized fermentation is prepared.

The invention provides an application of Oenococcus oeni in extracting aroma components from a raw material and an extraction method, belonging to the technical field of perfume processing. The application of Oenococcus oeni in extracting aroma components from a raw material and the extraction method mainly comprise preparing an enzyme preparation, enzymatically hydrolyzing a raw material with the enzyme preparation to obtain an enzymatic hydrolyzate, and then extracting aroma components from the enzymatic hydrolyzate by using a simultaneous distillation and extraction method; culturing Oenococcus oeni to prepare a cell disrupted liquid of the Oenococcus oeni, separating the cell disrupted liquid into a solid and a liquid to obtain a solid, and resuspending the solid to obtain an enzyme preparation; crushing the raw material and mixing it with a buffer solution to obtain a mixed liquid, and then adding the enzyme preparation for enzymatic hydrolysis to obtain an enzymatic hydrolyzate; and extracting aroma components from the enzymatic hydrolyzate by using a simultaneous distillation and extraction method to obtain an aroma component extract. The invention can effectively extract aroma components from the raw material, and in particular has a significant extraction effect on linalool, ionone, benzyl alcohol, and 2-phenylethanol components.