111 results about "Haematococcus pluvialis" patented technology

The invention discloses a method for extracting astaxanthin by using a biological enzyme, which comprises the following steps of: decomposing cell walls of haematococcus pluvialis at a certain temperature by using the biological enzyme, releasing astaxanthin from the haematococcus pluvialis, dissolving out the astaxanthin by using a certain solvent, and separating and purifying to obtain powdery astaxanthin. According to the method disclosed by the invention, wall breaking is carried out by combining ultrasound and biological enzyme, and a mixed solvent of ethanol and Tween 80 is adopted, so that the extraction rate of astaxanthin reaches 98.1%, the wall breaking time is shortened, and the efficiency is improved.

The invention relates to the technical field of microorganisms, and provides Haematococcus pluvialis RXXHp1 which is preserved in the China Center for Type Culture Collection on May 19, 2023, and the preservation number of the Haematococcus pluvialis RXXHp1 is CCTCC NO: M 2023791. The invention also provides an application of the haematococcus pluvialis RXXHp1. The application comprises the step of enriching at least one of organic selenium and astaxanthin in a selenium ion stress environment by using the haematococcus pluvialis RXXHp1. The invention further provides algae powder containing the haematococcus pluvialis RXXHp1 and application of the haematococcus pluvialis RXXHp1 or the algae powder in preparation of wine. The haematococcus pluvialis RXXHp1 provided by the invention has certain tolerance to a selenium ion stress environment, not only can be used for enriching organic selenium, but also can be used for promoting the accumulation of astaxanthin; besides, when the haematococcus pluvialis RXXHp1 is used for preparing the wine, the content of active substances such as astaxanthin and xanthophyll in the wine can be remarkably increased, so that the effects of improving the color and flavor of the wine and improving the oxidation resistance of the wine are achieved.

The invention belongs to the technical field of genetic engineering, and discloses a schizochytrium limacinum genetic engineering strain for enhancing supply of acetyl coenzyme A and efficiently producing astaxanthin, a method and application of the schizochytrium limacinum genetic engineering strain. By heterologous expression of a beta-carotene ketolase CrtO gene and a beta-carotene hydroxylase CrtZ gene from haematococcus pluvialis, synthesis of astaxanthin from beta-carotene in schizochytrium limacinum is promoted, so that the content of astaxanthin in schizochytrium limacinum HX-308 is increased. According to the invention, the endogenous AACT gene and heterologous CrtO, CrtZ, PK and PTA genes of schizochytrium limacinum are integrated into an original strain through homologous recombination, and the obtained strain still keeps genetic stability after multiple subculture, has the capability of efficiently synthesizing astaxanthin, effectively improves the yield of astaxanthin, and lays a foundation for large-scale industrial production.

The invention discloses an astaxanthin supercritical CO2 extraction process and special equipment, and relates to the field of astaxanthin preparation, the astaxanthin supercritical CO2 extraction process comprises a multistage wall-breaking granulation pretreatment stage and a dynamic countercurrent supercritical extraction stage, and liquid nitrogen low-temperature embrittlement is performed on a haematococcus pluvialis mud raw material; carrying out ultrahigh-pressure homogenization wall breaking on the algae mud subjected to low-temperature embrittlement to obtain wall-broken algae powder; carrying out vacuum belt type drying on the wall-broken algae powder, and removing moisture to a critical value; and preparing high-porosity particles from the dried wall-broken algae powder, porous starch, lecithin and nano calcium carbonate. In the multi-stage wall breaking pretreatment stage, cell walls of haematococcus pluvialis are efficiently destroyed under the combined action of liquid nitrogen low-temperature embrittlement and ultrahigh-pressure homogeneous wall breaking, so that astaxanthin wrapped in cells is fully exposed; according to the prepared high-porosity particles, the specific surface area and the pore structure of the particles are greatly increased, the contact efficiency of supercritical CO2 and astaxanthin is enhanced, the problems that in a traditional technology, the astaxanthin is high in wrapping rate and difficult to dissolve out are solved, and the extraction rate is remarkably increased.

The invention discloses a method for enhancing biomass and astaxanthin accumulated by haematococcus pluvialis based on gradient salt stress, and belongs to the technical field of bioengineering. According to the method, low-salt pre-stress is firstly carried out in the haematococcus pluvialis logarithmic culture stage to enhance the stress resistance of algae cells, and then high-salt is applied to induce the haematococcus pluvialis to efficiently accumulate biomass and astaxanthin in the stress stage. According to the method, the biomass of haematococcus pluvialis and the yield and stress resistance of astaxanthin can be effectively improved, the process is simple, operation is easy, dependence on the environment is small, the environment cannot be harmed in the culture process, and the method has a strong application prospect in large-scale production.

The invention discloses a method for promoting biomass accumulation of haematococcus pluvialis, which comprises the following steps: adding salicylic acid, melatonin and alpha-ketoglutaric acid into a basic culture medium, and replacing ferric trichloride with ferric citrate as an iron source to obtain an improved BG-11 culture medium; the method comprises the following steps: inoculating a haematococcus pluvialis seed solution into a culture bottle containing an improved culture medium, carrying out illumination treatment on the first day to the second day by adopting pulsed red light with the wavelength of 620 nm in the early stage of a green stage, carrying out illumination treatment on the third day to the fifth day by adopting red light with the wavelength of 660 nm in the middle stage of the green stage, and carrying out illumination treatment on the sixth day to the seventh day by adopting pulsed red light with the wavelength of 680 nm in the later stage of the green stage. Through a synergistic scheme of the improved BG-11 culture medium, staged red light treatment and dynamic environment regulation and control, the problems of low light energy utilization rate and insufficient stress resistance in the existing haematococcus pluvialis culture process are solved, high efficiency and stability of haematococcus pluvialis biomass accumulation are realized, the biomass can reach 7.0 * 10 < 5 > cells / mL in the later period of the green stage, and the yield of the haematococcus pluvialis can reach 7.0 * 10 < 5 > cells / mL in the later period of the green stage. And the survival rate of haematococcus pluvialis cells reaches 95%.

The invention relates to technical development and application of new special medical food on the basis of a new food raw material framework, and belongs to the technical field of special medical purpose formula food (FSMP). According to the series of foods, new food raw materials (euglena, haematococcus pluvialis and ampelopsis grossedentata leaves) are taken as cores, medicinal and edible components (lotus leaves, haws and mulberry leaves) and marine active substances (fucoidan polysaccharides and chitin) are combined, functional factors are extracted through a prehydrolysis SBE technology and a biological enzymolysis technology, and the bioavailability is improved by adopting a nano-carrier technology. The product comprises a total nutrient base material (20-30% of protein and 15-25% of dietary fiber), functional components (10-15% of a new food raw material extract and 8-12% of a traditional Chinese medicine extract) and a metabolism regulator (conjugated linoleic acid and L-carnitine). Clinical tests show that after an obese patient takes 400 kcal of the replacement meal every day, the weight of the obese patient is reduced by 10.2 kg on average (4.8 kg of a control group) 12 weeks later, and the body fat rate is reduced by 16.3%. The product supports line iteration (such as powder, tablets and liquid food) through modular formula design, and fills the application blank of new food raw materials in special medical food.

The application provides a method for synchronously separating active substances of Haematococcus pluvialis, and relates to the technical field of natural product extraction, and comprises an extraction phase with a mass ratio of 20%-50%, and the balance is water; the extraction phase is a quaternary phosphonium ionic liquid or a eutectic solvent; the quaternary phosphonium ionic liquid comprises tetrabutylphosphonium trifluoroacetate or tributyl octyl phosphonium bromide; and the eutectic solvent comprises a quaternary phosphonium ionic liquid and a fatty acid with a molar ratio of 1:1-5:3. The extraction phase has weak acidity, the ability to form van der Waals forces and hydrogen bond interactions, the Haematococcus pluvialis cell wall breaking effect and high solubility. The extraction phase can form liquid-liquid phase separation, can realize the synchronous extraction of Haematococcus pluvialis cell wall breaking, astaxanthin, carbohydrates and proteins, and has no organic solvent, is simple to operate, has short extraction time and high extraction efficiency. The method solves the technical problem that the extraction agent in the prior art cannot effectively separate multiple active substances of Haematococcus pluvialis.

The invention relates to a method for directionally improving the tolerance of haematococcus pluvialis acetate, aiming at the problem that haematococcus pluvialis is sensitive to high-concentration acetate, the method comprises the following steps: gradually improving the tolerance of algae species through a staged adaptive evolution strategy, gradually adapting WT to 2.5 g / L sodium acetate through short-period-multi-cycle domestication, and obtaining a domesticated algae species HpAC25; on the basis of HpAC25, domesticating the HpAC25 to 5g / L to obtain HpAC50; further, on the basis of HpAC50, domesticating to obtain 7.5 g / L of sodium acetate, so as to obtain HpAC75. In the domestication process, suspended green motile cells are enriched through centrifugation, circulation passage is carried out every 3 days, the cell density is monitored, and the accumulative culture period reaches 189 days. Experiments show that the domesticated algae species HpAC50 and HpAC75 are cultured for 10 days under 5g / L sodium acetate and 7.5 g / L sodium acetate, and the survival rates of the domesticated algae species HpAC50 and HpAC75 reach 81.22% and 43.51% respectively and are increased by 422% and 460% compared with WT. The method is simple and convenient to operate, the tolerance of haematococcus pluvialis to acetate is remarkably enhanced, and a technical basis is provided for efficient production of astaxanthin and large-scale culture.

The invention discloses a haematococcus pluvialis culture illumination system based on edge control and a control method, and relates to the technical field of haematococcus pluvialis culture illumination control, and the haematococcus pluvialis culture illumination system comprises a plurality of sensor monitoring modules, a plurality of edge controllers, a plurality of illumination units and a digital twinborn monitoring platform. According to the method, the illumination intensity can be dynamically adjusted according to the actual growth state of algae, and the problem of algae growth inhibition or insufficient astaxanthin accumulation possibly caused in a traditional fixed illumination control mode is solved. All haematococcus pluvialis cultivation is divided into a plurality of areas for more precise control, an edge controller is arranged in each set area range, and the edge controllers can optimize illumination modes in the area ranges based on real-time data analysis. The digital twinborn monitoring platform establishes a three-dimensional digital model of haematococcus pluvialis growth by collecting data of the sensor monitoring module and the edge controller in real time, and real-time visual monitoring is achieved.

The application discloses a functional composition for improving bone and joint health, a conditioner and application. The functional composition comprises a first active component and a second active component; the first active component comprises elastin peptide, haematococcus pluvialis and ergothioneine; and the second active component comprises one or more of houttuynia cordata and non-denatured type II collagen. The application realizes the protection effect on joint, cartilage and bone health by scientific matching of the components and synergistically improving the glycosaminoglycan level in chondrocytes and the osteocalcin level in osteoblasts. Meanwhile, the components in the functional composition for improving bone and joint health are natural and have good biocompatibility, and have no obvious side effects, so that the application provides a kind of drug or a substitute for the treatment and prevention of bone diseases.

The invention discloses a treatment method of fermentation wastewater. The treatment method of the fermentation wastewater comprises the following steps: adding filamentous fungi and green algae into fermentation wastewater generated after fermentation treatment of kitchen garbage, carrying out fermentation culture, then carrying out solid-liquid separation, and extracting grease from the separated solid. The filamentous fungi comprise trichoderma harzianum, and the green algae comprise one or a combination of more selected from chlorella vulgaris, chlamydomonas, haematococcus pluvialis, hylothrix, scenedesmus obliquus and chaetoclada. According to the treatment method of the fermentation wastewater, grease can be produced while the fermentation wastewater is treated, and the treatment cost of the fermentation wastewater is reduced.

This invention discloses a multi-level astaxanthin complex, its preparation method, and its applications, relating to the fields of encapsulation technology or astaxanthin formulations. It includes a first encapsulation component containing astaxanthin and a second encapsulation component containing Haematococcus pluvialis isolated protein; optionally, the multi-level astaxanthin complex further contains cyclodextrin or its derivatives. This invention overcomes the problems of aggregation and uneven encapsulation caused by the direct addition of astaxanthin or astaxanthin solution and the need for vigorous stirring to promote the formation of encapsulated particles in existing astaxanthin complex preparation techniques through local microencapsulation in a foaming microenvironment.

The invention discloses a multifunctional special dietary food skin beautifying and shaping formula and application, and belongs to the technical field of beauty treatment. The multifunctional special dietary food at least comprises collagen peptide, collagen tripeptide, skipjack elastin peptide, carob bean extract, phosphatidylserine, casein hydrolytic peptide, nicotinamide, rice bran oil powder, haematococcus pluvialis, cranberry powder, oriental cherry flower powder, pig blood peptide powder, red date peptide powder and cattle spleen peptide. The health-care product can comprehensively supplement nutrition recovered after exercise, has the effects of beautifying, shaping and removing wrinkles, can effectively relieve pressure and regulate emotion, and also has the effects of improving female physiological health, strengthening the spleen and stomach and enhancing immunity.

The application discloses a kind of NADES and method for extracting astaxanthin from haematococcus pluvialis using it, wherein a kind of natural eutectic solvent for extracting astaxanthin from haematococcus pluvialis includes 1 molar part of hydrogen bond acceptor, 2-6 molar parts of hydrogen bond donor;The hydrogen bond acceptor is choline chloride, and the hydrogen bond donor is 1,4-butanediol or 1,2-propanediol.The extraction solvent of the application only needs to use the above-mentioned natural eutectic solvent to realize the extraction of astaxanthin in haematococcus pluvialis, which has the advantages of safety, green, higher extraction efficiency, etc., and is very beneficial to the industrialization and large-scale production of astaxanthin.

The application discloses a fermentation wastewater treatment method. The fermentation wastewater treatment method comprises the following steps: adding filamentous fungi and green algae into fermentation wastewater generated after fermentation treatment of kitchen garbage to carry out fermentation culture, then carrying out solid-liquid separation, and extracting oil from the separated solid. The filamentous fungi comprise Trichoderma harzianum, and the green algae comprise one or more selected from the group consisting of Chlorella vulgaris, Chlorella sp., Haematococcus pluvialis, Leptolyngbya sp., Agmenellum sp. and Comatulicium sp. The fermentation wastewater treatment method can produce oil while treating fermentation wastewater, and reduces the cost of fermentation wastewater treatment.

The invention discloses a functional composition capable of improving bone and joint health, a conditioner and application. The functional composition comprises a first active component and a second active component, the first active component is prepared from elastin peptide, haematococcus pluvialis and ergothioneine; the second active component is prepared from one or more of herba houttuyniae and non-denatured type II collagen. Through scientific compatibility of all the components, the levels of glycosaminoglycan in chondrocytes and osteocalcin in osteoblasts are synergistically improved, and the protection effect on joint, cartilage and skeleton health is achieved. Meanwhile, a plurality of components in the functional composition capable of improving the health of the bones and the joints are natural in source, good in biocompatibility and free of obvious side effects, and a medicine or an alternative scheme thereof is provided for treatment and prevention of the bone diseases.

The invention provides a haematococcus pluvialis freeze-dried algae seed production process which comprises the following steps: algae seed acquisition: automatically separating haematococcus pluvialis seeds from a natural water body water sample by adopting a flow cytometry sorting technology in combination with a fluorescence labeling method; expanding cultivation: adopting an open autotrophy mode, and automatically adjusting the illumination intensity and duration by utilizing an intelligent control system; concentrating, namely concentrating the algae liquid into algae mud through a centrifugal machine; compounding and blending, namely adding a freeze-drying protective agent into the concentrated algae seeds, and uniformly mixing; and filling and storing, namely packaging by adopting nitrogen, and storing in a shady and dry place for a long time. According to the method, flow cytometry sorting is combined with a fluorescence labeling method to separate algae species, the expanding culture environment is intelligently regulated and controlled, a freeze-drying protective agent formula and a vacuum freezing nitrogen packaging process are optimized, the dormant state and long-term stable storage of the algae species are achieved, the storage stability and transportation convenience of the algae species are improved, and meanwhile it is guaranteed that the algae species can be revived with water; and adding a culture medium for propagation and propagation.

The invention relates to a grouper crossbreeding method. The method comprises the four steps of parent breeding, temperature-sensitive domestication, artificial spawning and insemination, incubation and fry breeding, specifically, cold water-based clear water stone spots and hot water-based brown spot stone spots are selected as parents, the clear water stone spots are used as female parents, the brown spot stone spots are used as male parents, and the middle water temperature is adapted through gradient temperature-sensitive domestication; stress-resistant feed containing hilsa herring egg source peptide and haematococcus pluvialis astaxanthin ester and a temperature-sensitive blender are matched, a reproductive care agent is supplemented to strengthen sperm and egg performance, and normative spawning induction, insemination and graded cultivation processes are adopted. According to the method, the problems of poor water temperature adaptation and low hatching rate of grouper hybrid sperms and eggs in different temperature areas are solved, the wide-temperature property of filial generations is improved, the method adapts to the water temperature of 18-28 DEG C, the hatching rate, the fry survival rate, the adult fish survival rate and the average weight of single fish are remarkably improved, and technical support is provided for industrial application.

The present invention discloses a composition for improving sleep, preventing and treating anxiety, delaying aging and its preparation method. The composition includes lily, lemon balm, liquorice, mulberry, Poria, spinach seed, sea buckthorn, lotus seed core, roxburghii, alpinia oxyphylla, valerian root, acanthopanax senticosus, red rose, light bamboo leaf, snow lotus, gastrodia elata, passion flower, ergothioneine, dihydroquercetin, gamma-aminobutyric acid, casein peptide, royal jelly, pea peptide, tea theanine, fish collagen peptide and Haematococcus pluvialis powder. The composition of the present invention rationally proportions each component, and patient takes and is easy to carry, and is effective. In addition, the preparation method process of the composition is simple, stable and reliable in performance, high in production efficiency, low in production cost, and has broad application prospects.

The present invention relates to the field of astaxanthin oil, specifically to a method for preparing astaxanthin oil with high anti-inflammatory activity and its application. The present invention cultivates Haematococcus pluvialis H7 and Haematococcus pluvialis ENN71 in a racetrack photobioreactor and a pipeline photobioreactor to accumulate astaxanthin and prepare astaxanthin oil. Under the same concentration and conditions, the combined culture significantly enhances the anti-inflammatory activity of astaxanthin oil compared to astaxanthin oil prepared by culturing Haematococcus pluvialis H7 or Haematococcus pluvialis ENN71 alone, increasing the zebrafish embryo neutrophil aggregation inhibition rate by 76.2% and 115.2%, respectively. The present application optimizes the ratio of the combined culture of Haematococcus pluvialis H7 and Haematococcus pluvialis ENN71. When the mass ratio of Haematococcus pluvialis H7 to Haematococcus pluvialis ENN71 is 1:2, the astaxanthin oil prepared by the combined culture has the highest anti-inflammatory activity, with a zebrafish embryo neutrophil aggregation inhibition rate of 77.1±0.36%.

The invention is suitable for the technical field of biological medicine, and provides an active composition for relieving fatigue and application of the active composition. The composition is prepared from a silkworm chrysalis-derived mitochondrial repair peptide, a chickpea-derived neuromodulation peptide, a haematococcus pluvialis-derived phagocytosis peptide, a phosphatidylcholine-lipoic acid conjugate, a whey protein-chitosan copolymer and an ergothioneine-selenium compound in a synergistic manner. Through the synergistic effect of four mechanisms of energy metabolism activation, endocrine balance, mitochondrial autophagy repair and oxidative stress removal, physiological fatigue and stress fatigue are effectively and synchronously relieved. After targeted modification, the utilization rate of macamides is remarkably increased, and the composition can remarkably prolong exercise tolerance, reduce cortisol level, promote GABA synthesis, recover mitochondrial function and greatly shorten fatigue recovery time.

The invention discloses a composite hydrogel system loaded with haematococcus pluvialis exosomes and a preparation method and application of the composite hydrogel system, the haematococcus pluvialis exosomes are extracted and encapsulated in hydrogel formed by mixing carboxymethyl chitosan, oxidized sodium alginate, polyvinyl alcohol and tannic acid, and the composite hydrogel system loaded with the haematococcus pluvialis exosomes is prepared. The hydrogel system has injectability and tissue adhesion, slowly releases haematococcus pluvialis exosome, effectively promotes healing of diabetic wounds and reduces scars after healing. The hydrogel system has the advantages of good biocompatibility, low cost, simple preparation process and easiness in large-scale production, and has a wide application prospect in the field of chronic wounds of diabetes mellitus.

The utility model relates to a physical method nanometer material stripping and dispersing machine, which belongs to the technical field of nanometer material mechanical stripping and dispersing and comprises a jacket stirring barrel, a high-pressure jet flow mechanism, a liquid-accumulation-free material taking mechanism, an explosion-proof electric cabinet integrated system and an air filtering and drying machine for a transducer. A first I-level frequency conversion explosion-proof motor is arranged at the top of the jacketed stirring barrel, a right-angle speed reducer is fixedly mounted on the top surface of the motor base, the output end of the first I-level frequency conversion explosion-proof motor is fixedly connected with the right-angle speed reducer, and a quick lock catch is arranged on the jacketed stirring barrel. According to the physical method nano material stripping dispersion machine, the cost is reduced, the efficiency is improved, the problems of high preparation cost, low efficiency, poor consistency and the like in the new material industry are solved, a guarantee is provided for effectively extracting astaxanthin from haematococcus pluvialis through wall breaking and guaranteeing the biological activity of the astaxanthin, and the problem that existing equipment has tube breaking pain points in preparation of carbon nano tubes is solved; and the explosion risk caused by mixing and dispersing the NMP in the carbon nanotube slurry is solved.

This invention relates to the field of photocatalysis technology, and more particularly to a Haematococcus pluvialis / CdS composite material, its preparation method, and its applications. The Haematococcus pluvialis / CdS composite material provided by this invention comprises Haematococcus pluvialis and nano-CdS coated on the surface of the Haematococcus pluvialis; the mass content of astaxanthin in the Haematococcus pluvialis is 1-10%. Haematococcus pluvialis contains astaxanthin, which can enhance the ability of CdS to capture photons within the visible light wavelength range, thus acting as a dye sensitizer, while simultaneously inhibiting the recombination of photogenerated electrons / holes and improving the transport and transfer efficiency of photogenerated electrons. Therefore, compared with CdS alone, the Haematococcus pluvialis / CdS composite material exhibits better visible light photocatalytic activity. Results show that the Haematococcus pluvialis / CdS composite material has good visible light photocatalytic performance, achieving a removal rate of 84.5% for the recalcitrant norfloxacin within 60 min, and exhibits good cycling stability.

The haematococcus pluvialis culture pond comprises a base, a pond body is arranged at the top of the base, stirring rods are arranged at the bottoms of the two sides of the interior of the pond body in a sleeved mode, U-shaped connecting rods are arranged on the two sides of each stirring rod, a driving assembly in transmission connection with one set of U-shaped connecting rods is arranged on one side of the top of the base, and the other side of the top of the base is provided with a driving assembly in transmission connection with the other set of U-shaped connecting rods. Sleeves are arranged on the two sides of the base. Through mutual cooperation of the driving assembly, the movable rod, the sleeve, the hinge rod, the U-shaped connecting rod, the liquid discharging pipe, the filter screen, the filter box and the piston, when the device drives the stirring rod to stir liquid in a culture pond, the piston can be synchronously driven to do piston operation in the sleeve, and the liquid in the pond body is continuously pumped into the filter box to be filtered; the filtered liquid can be conveyed into the pond body for circulation, so that impurities in the culture pond can be automatically removed, the cleanliness in the culture pond is ensured, and the impurity treatment convenience is improved.

The application provides a preparation method of astaxanthin cordyceps militaris fine tablets, comprising the following steps: subjecting cordyceps militaris fruiting bodies to supercritical CO2 extraction treatment to separate a cordyceps militaris terpene component; mixing Haematococcus pluvialis powder and edible vegetable oil, adding the cordyceps militaris terpene component and rosemary extract, and cooling and forming to obtain an astaxanthin-cordyceps militaris terpene blend; crushing the residue after extraction, adding purified water and a composite enzyme preparation to perform enzymolysis to obtain an enzymolysis liquid; adding a chitosan quaternary ammonium salt solution, centrifuging after flocculation, vacuum drying, and crushing to obtain cordyceps militaris active powder; mixing and granulating raw materials to obtain granulation particles; uniformly mixing the granulation particles with xylitol and microcrystalline cellulose, and pressing into tablets; and placing the tablets in a coating machine for coating. The application realizes efficient retention of active ingredients, improvement of safety and stability of product quality through the cooperation of multiple technologies such as supercritical CO2 extraction, enzymolysis flocculation, low-temperature fluidized bed granulation and hot melt coating.

The invention provides aquarium fish feed for gullfish and a preparation method of the aquarium fish feed, and relates to the technical field of aquatic product feeding. The aquarium fish feed is prepared from hermetia illucens larva powder, white fish meal, haematococcus pluvialis wall-broken powder, lycium barbarum polysaccharide extract, squid viscera hydrolysate, compound vitamin premix, mineral premix, yeast beta-glucan, Chinese herbal medicine extract, compound probiotics and sodium alginate. According to the invention, the defects in the prior art are overcome, the insect protein powder is used for replacing fish meal / cattle heart, natural color enhancing components are added, and probiotics and Chinese herbal medicines are combined for compounding, so that the synergistic effect of nutrition, environmental protection and functionality is realized, the growth of the symphysodon is effectively promoted, and water pollution is reduced.

The invention relates to the technical field of microalgae culture, in particular to an intelligent haematococcus pluvialis culture system and method for waste biogas slurry resource utilization, and the system comprises a biogas slurry collection module which is used for collecting waste biogas slurry; the biogas slurry treatment and culture medium preparation module is used for carrying out treatment and nutrition blending on waste biogas slurry to prepare a culture medium special for haematococcus pluvialis; the modular culture unit is used for bearing a special culture medium and culturing haematococcus pluvialis for green growth of algae cells; the intelligent control module is used for receiving real-time monitoring data in the breeding process and dynamically optimizing key parameters such as illumination, temperature, pH, gas flow and nutrition supply through a self-adaptive algorithm; the induction harvesting module is used for applying multiple stress to the high-density algae liquid to induce astaxanthin accumulation, and algae mud is collected through a secondary harvesting system. Therefore, the problems of high production cost, low culture efficiency and the like in the prior art are solved.

The application discloses a kind of complex small molecule coral food and its application in adjusting coral symbiotic microbial community structure.The raw materials of the complex small molecule coral food include: soybean meal small peptide protein, cod small peptide protein, haematococcus pluvialis powder, complex amino acid, squid meal, chitosan oligosaccharide and complex nucleotide.The complex small molecule coral food of the application takes soybean meal small peptide protein and cod small peptide protein as main component, haematococcus pluvialis as secondary component, and is supplemented with complex amino acid and squid meal, and is additionally added with chitosan oligosaccharide and complex nucleotide.The obtained complex small molecule coral food not only has good palatability, but also ensures the feeding amount of coral.It is verified by test that the complex small molecule coral food of the application can adjust the symbiotic microbial community structure of aquarium coral, improve the proportion of coral beneficial symbiotic bacteria, and further improve the environmental tolerance of coral.