144 results about "Haematococcus pluvialis" patented technology

The invention discloses a high-temperature-resistant astaxanthin microcapsule and a preparation method and application thereof, and relates to the field of nutritional products, the microcapsule comprises: a, a core material: astaxanthin oil selected from haematococcus pluvialis or krill oil; b, double-layer wall materials: an inner-layer wall material is formed by crosslinking sodium alginate and whey protein through an ionic gel method; the outer-layer wall material is formed by electrostatic adsorption of chitosan and Arabic gum; and c, an antioxidant synergist: a rosemary extract. A double-layer embedding structure is adopted outside astaxanthin oil, sodium alginate on the inner layer is combined with whey protein to form a compact network structure through ionic bonds, chitosan on the outer layer and Arabic gum form a compact composite film on the surface of a primary embedded microsphere through electrostatic attraction, so that the double-layer embedded microcapsule has the advantages that the astaxanthin oil has good stability, and the astaxanthin oil has good stability. The temperature resistance limit of a traditional single-layer microcapsule is broken through, the stability of the astaxanthin in a high-temperature baking environment is remarkably improved, the astaxanthin can stably exist in high-temperature baked food, and the anti-oxidation effect and the like of the astaxanthin are fully exerted.

The invention discloses a method for extracting astaxanthin by using a biological enzyme, which comprises the following steps of: decomposing cell walls of haematococcus pluvialis at a certain temperature by using the biological enzyme, releasing astaxanthin from the haematococcus pluvialis, dissolving out the astaxanthin by using a certain solvent, and separating and purifying to obtain powdery astaxanthin. According to the method disclosed by the invention, wall breaking is carried out by combining ultrasound and biological enzyme, and a mixed solvent of ethanol and Tween 80 is adopted, so that the extraction rate of astaxanthin reaches 98.1%, the wall breaking time is shortened, and the efficiency is improved.

The invention provides a haematococcus pluvialis culture medium and a culture method thereof. The culture medium comprises a nutrition proliferation culture medium, a stress induction culture medium and an accumulation strengthening culture medium which are used for segmented culture, the nutrition proliferation culture medium comprises sodium nitrate, monopotassium phosphate, potassium chloride, ferric ammonium citrate, sodium citrate and a trace element mixed solution; adding an osmotic stress agent, an oxidation inducer and plant hormones into a stress induction culture medium; and accumulating and strengthening the culture medium to remove sodium nitrate, and adding and supplementing a carbon source, a polymer penetrant and a zwitterionic stabilizer. The culture method comprises the following steps: a nutrition proliferation stage, a stress induction stage and an accumulation strengthening stage. According to the method, through a three-section culture medium and a fine regulation culture process, and through the synergistic effect of a composite stress factor and a dynamic environment signal, on the premise of ensuring relatively high activity of algae cells, bidirectional synergistic efficient improvement of biomass growth and astaxanthin accumulation is realized.

The invention relates to the technical field of microorganisms, and provides Haematococcus pluvialis RXXHp1 which is preserved in the China Center for Type Culture Collection on May 19, 2023, and the preservation number of the Haematococcus pluvialis RXXHp1 is CCTCC NO: M 2023791. The invention also provides an application of the haematococcus pluvialis RXXHp1. The application comprises the step of enriching at least one of organic selenium and astaxanthin in a selenium ion stress environment by using the haematococcus pluvialis RXXHp1. The invention further provides algae powder containing the haematococcus pluvialis RXXHp1 and application of the haematococcus pluvialis RXXHp1 or the algae powder in preparation of wine. The haematococcus pluvialis RXXHp1 provided by the invention has certain tolerance to a selenium ion stress environment, not only can be used for enriching organic selenium, but also can be used for promoting the accumulation of astaxanthin; besides, when the haematococcus pluvialis RXXHp1 is used for preparing the wine, the content of active substances such as astaxanthin and xanthophyll in the wine can be remarkably increased, so that the effects of improving the color and flavor of the wine and improving the oxidation resistance of the wine are achieved.

The present disclosure relates to the field of microbial technology and provides a Haematococcus pluvialis KM2-1 and its applications. The present disclosure provides a Haematococcus pluvialis ( Haematococcus lacustris ) KM2-1, which was deposited at the China Center for Type Culture Collection on April 3, 2025, and its deposit number is CCTCC NO: M 2025704; the present disclosure also provides an application of Haematococcus pluvialis KM2-1 in enriching carotenoids; the present disclosure also provides an application of Haematococcus pluvialis KM2-1 in preparing products rich in carotenoids. The Haematococcus pluvialis KM2-1 provided by the present disclosure has good tolerance to light stress and can promote the accumulation of carotenoids under high-intensity light stress conditions, thereby better enriching carotenoids.

The invention belongs to the technical field of genetic engineering, and discloses a schizochytrium limacinum genetic engineering strain for enhancing supply of acetyl coenzyme A and efficiently producing astaxanthin, a method and application of the schizochytrium limacinum genetic engineering strain. By heterologous expression of a beta-carotene ketolase CrtO gene and a beta-carotene hydroxylase CrtZ gene from haematococcus pluvialis, synthesis of astaxanthin from beta-carotene in schizochytrium limacinum is promoted, so that the content of astaxanthin in schizochytrium limacinum HX-308 is increased. According to the invention, the endogenous AACT gene and heterologous CrtO, CrtZ, PK and PTA genes of schizochytrium limacinum are integrated into an original strain through homologous recombination, and the obtained strain still keeps genetic stability after multiple subculture, has the capability of efficiently synthesizing astaxanthin, effectively improves the yield of astaxanthin, and lays a foundation for large-scale industrial production.

The invention discloses an astaxanthin supercritical CO2 extraction process and special equipment, and relates to the field of astaxanthin preparation, the astaxanthin supercritical CO2 extraction process comprises a multistage wall-breaking granulation pretreatment stage and a dynamic countercurrent supercritical extraction stage, and liquid nitrogen low-temperature embrittlement is performed on a haematococcus pluvialis mud raw material; carrying out ultrahigh-pressure homogenization wall breaking on the algae mud subjected to low-temperature embrittlement to obtain wall-broken algae powder; carrying out vacuum belt type drying on the wall-broken algae powder, and removing moisture to a critical value; and preparing high-porosity particles from the dried wall-broken algae powder, porous starch, lecithin and nano calcium carbonate. In the multi-stage wall breaking pretreatment stage, cell walls of haematococcus pluvialis are efficiently destroyed under the combined action of liquid nitrogen low-temperature embrittlement and ultrahigh-pressure homogeneous wall breaking, so that astaxanthin wrapped in cells is fully exposed; according to the prepared high-porosity particles, the specific surface area and the pore structure of the particles are greatly increased, the contact efficiency of supercritical CO2 and astaxanthin is enhanced, the problems that in a traditional technology, the astaxanthin is high in wrapping rate and difficult to dissolve out are solved, and the extraction rate is remarkably increased.

The present invention relates to the technical field of Haematococcus pluvialis cultivation, and specifically relates to a disinfection device for a Haematococcus pluvialis cultivation system, which includes an algae storage tank, a disinfection tank, and a number of cultivation tubes. A separation part is connected and arranged between two adjacent upper and lower ones among a number of cultivation tubes on the same side. By starting the double-headed motor to drive the partition to rotate 90 degrees, a number of liquid passing boxes and connecting tubes are blocked, so as to cut off two adjacent cultivation tubes at the same height, and at the same time, the two adjacent upper and lower cultivation tubes are connected to form a loop path. Then, the disinfectant with a large density sinks into the lower cultivation tube to form a layer, and it can be pumped and drained, while the Haematococcus pluvialis culture solution is retained in the cultivation tube for continuous cultivation. Then, just open the liquid outlet pipe to release the disinfectant. Therefore, it is not necessary to lead out and collect all the Haematococcus pluvialis culture solution and then separate the disinfectant. This device saves the time and process of disinfection and separation, and avoids the long-term adverse effects of the disinfectant on the Haematococcus pluvialis culture solution.

The invention discloses a method for enhancing biomass and astaxanthin accumulated by haematococcus pluvialis based on gradient salt stress, and belongs to the technical field of bioengineering. According to the method, low-salt pre-stress is firstly carried out in the haematococcus pluvialis logarithmic culture stage to enhance the stress resistance of algae cells, and then high-salt is applied to induce the haematococcus pluvialis to efficiently accumulate biomass and astaxanthin in the stress stage. According to the method, the biomass of haematococcus pluvialis and the yield and stress resistance of astaxanthin can be effectively improved, the process is simple, operation is easy, dependence on the environment is small, the environment cannot be harmed in the culture process, and the method has a strong application prospect in large-scale production.

The invention discloses a method for promoting biomass accumulation of haematococcus pluvialis, which comprises the following steps: adding salicylic acid, melatonin and alpha-ketoglutaric acid into a basic culture medium, and replacing ferric trichloride with ferric citrate as an iron source to obtain an improved BG-11 culture medium; the method comprises the following steps: inoculating a haematococcus pluvialis seed solution into a culture bottle containing an improved culture medium, carrying out illumination treatment on the first day to the second day by adopting pulsed red light with the wavelength of 620 nm in the early stage of a green stage, carrying out illumination treatment on the third day to the fifth day by adopting red light with the wavelength of 660 nm in the middle stage of the green stage, and carrying out illumination treatment on the sixth day to the seventh day by adopting pulsed red light with the wavelength of 680 nm in the later stage of the green stage. Through a synergistic scheme of the improved BG-11 culture medium, staged red light treatment and dynamic environment regulation and control, the problems of low light energy utilization rate and insufficient stress resistance in the existing haematococcus pluvialis culture process are solved, high efficiency and stability of haematococcus pluvialis biomass accumulation are realized, the biomass can reach 7.0 * 10 < 5 > cells / mL in the later period of the green stage, and the yield of the haematococcus pluvialis can reach 7.0 * 10 < 5 > cells / mL in the later period of the green stage. And the survival rate of haematococcus pluvialis cells reaches 95%.

The invention relates to technical development and application of new special medical food on the basis of a new food raw material framework, and belongs to the technical field of special medical purpose formula food (FSMP). According to the series of foods, new food raw materials (euglena, haematococcus pluvialis and ampelopsis grossedentata leaves) are taken as cores, medicinal and edible components (lotus leaves, haws and mulberry leaves) and marine active substances (fucoidan polysaccharides and chitin) are combined, functional factors are extracted through a prehydrolysis SBE technology and a biological enzymolysis technology, and the bioavailability is improved by adopting a nano-carrier technology. The product comprises a total nutrient base material (20-30% of protein and 15-25% of dietary fiber), functional components (10-15% of a new food raw material extract and 8-12% of a traditional Chinese medicine extract) and a metabolism regulator (conjugated linoleic acid and L-carnitine). Clinical tests show that after an obese patient takes 400 kcal of the replacement meal every day, the weight of the obese patient is reduced by 10.2 kg on average (4.8 kg of a control group) 12 weeks later, and the body fat rate is reduced by 16.3%. The product supports line iteration (such as powder, tablets and liquid food) through modular formula design, and fills the application blank of new food raw materials in special medical food.

The invention belongs to the field of industrial application of microalgae, and provides a method for improving astaxanthin produced by haematococcus pluvialis. Comprising the following steps: 1, culturing haematococcus pluvialis to a logarithmic phase; 2, the haematococcus pluvialis is diluted to a proper concentration, and a seed solution for producing astaxanthin from the haematococcus pluvialis is prepared; 3, respectively adding six pre-prepared inducers including creatine, glycine, 6-aminocaproic acid, glutamic acid, L-hydroxyproline and L-cysteine into the prepared haematococcus pluvialis liquid for induction; and 4, continuously filling sterile air into the haematococcus pluvialis liquid added with the inducer in a photobioreactor, culturing, and inducing haematococcus pluvialis cells to accumulate astaxanthin. The invention discloses a method for improving astaxanthin produced by haematococcus pluvialis. The haematococcus pluvialis can be promoted to accumulate a large amount of astaxanthin, and the production cycle of the astaxanthin is shortened. The method is easy to operate, high in efficiency, low in cost and completely suitable for industrial culture of haematococcus pluvialis.

The invention relates to the technical field of natural product extraction, and particularly discloses a method for extracting astaxanthin from haematococcus pluvialis. According to the method, a deep eutectic solvent (DES) composed of sodium alpha-ketoglutarate and lactic acid is adopted as an extraction medium, the molar ratio of the sodium alpha-ketoglutarate to the lactic acid is 1: 1-1: 4, water bath ultrasonic extraction is carried out at the temperature of 25-50 DEG C, and the liquid-solid ratio is 10: 1-40: 1. Experiments show that under the optimal condition, the astaxanthin extraction rate and the DPPH free radical scavenging rate are obviously superior to those of a traditional solvent method. The method has the characteristics of greenness, high efficiency and simplicity and convenience in operation, and provides a new strategy for astaxanthin extraction.

The invention discloses double-embedded microcapsule powder as well as a formula, a preparation method and application thereof, and belongs to the technical field of foods. According to the invention, phase condensation is carried out on core materials with different polarities, so that microcapsule powder capable of pairwise embedding the core materials with different polarities, lipophilic core materials such as vegetable oil (conjugated linoleic acid glyceride, medium chain triglyceride, DHA algal oil, linseed oil, sunflower seed oil and the like), animal oil, haematococcus pluvialis oil or fat-soluble vitamins and the like are obtained; the hydrophilic core materials comprise water-soluble vitamins, high-solubility amino acids and the like, the amphiphilic core materials comprise diglyceride oil, phospholipid oil, sugar fat oil and the like, and the amphoteric insoluble core materials comprise polyphenol substances or low-solubility amino acid substances and the like. According to the microcapsule powder and the preparation method thereof, diversification and compatibility of properties of the core material are realized, the corresponding microcapsule powder effectively isolates negative effects of external environmental factors such as oxygen, moisture, illumination and temperature change on the core material, and the microcapsule powder can be widely applied to industries such as medicine, food and cosmetics.

The invention discloses a preparation method of astaxanthin nanoemulsion, and belongs to the field of astaxanthin, the preparation method comprises the following steps: S1, heating 120-150 parts by mass of haematococcus pluvialis oil to 28-30 DEG C, keeping the temperature, and continuously stirring for later use to obtain a component A; s2, dissolving 4-7 parts by mass of a polygonatum odoratum extract and 3-5 parts by mass of a black pepper extract in 5-20 parts by mass of purified water, and heating to 28-30 DEG C to obtain a component B; s3, emulsification treatment: mixing the component A and the component B according to a mass ratio of 1: (1-2), adding a compound emulsifier which is 1-3 times of the mass of the mixture, uniformly stirring, treating by using a high-speed dispersion homogenizer, and then treating by using an ultrasonic cell disruptor; when a high-speed dispersion homogenizer is used for treatment, high-speed shearing dispersion is carried out for 9-15 min at the speed of 10000 r / min to 12000 r / min; when an ultrasonic cell disruptor is used for treatment, ultrasonic dispersion is carried out for 2 min at the power of 100 W, then ultrasonic dispersion is carried out for 4 min at the power of 200 W, the mode of working for 1 s and intermittent for 1 s is selected, and the astaxanthin nanoemulsion is obtained. The nano-emulsion prepared by the invention is good in stability and high in bioavailability.

The application provides a method for synchronously separating active substances of Haematococcus pluvialis, and relates to the technical field of natural product extraction, and comprises an extraction phase with a mass ratio of 20%-50%, and the balance is water; the extraction phase is a quaternary phosphonium ionic liquid or a eutectic solvent; the quaternary phosphonium ionic liquid comprises tetrabutylphosphonium trifluoroacetate or tributyl octyl phosphonium bromide; and the eutectic solvent comprises a quaternary phosphonium ionic liquid and a fatty acid with a molar ratio of 1:1-5:3. The extraction phase has weak acidity, the ability to form van der Waals forces and hydrogen bond interactions, the Haematococcus pluvialis cell wall breaking effect and high solubility. The extraction phase can form liquid-liquid phase separation, can realize the synchronous extraction of Haematococcus pluvialis cell wall breaking, astaxanthin, carbohydrates and proteins, and has no organic solvent, is simple to operate, has short extraction time and high extraction efficiency. The method solves the technical problem that the extraction agent in the prior art cannot effectively separate multiple active substances of Haematococcus pluvialis.

The invention relates to a method for directionally improving the tolerance of haematococcus pluvialis acetate, aiming at the problem that haematococcus pluvialis is sensitive to high-concentration acetate, the method comprises the following steps: gradually improving the tolerance of algae species through a staged adaptive evolution strategy, gradually adapting WT to 2.5 g / L sodium acetate through short-period-multi-cycle domestication, and obtaining a domesticated algae species HpAC25; on the basis of HpAC25, domesticating the HpAC25 to 5g / L to obtain HpAC50; further, on the basis of HpAC50, domesticating to obtain 7.5 g / L of sodium acetate, so as to obtain HpAC75. In the domestication process, suspended green motile cells are enriched through centrifugation, circulation passage is carried out every 3 days, the cell density is monitored, and the accumulative culture period reaches 189 days. Experiments show that the domesticated algae species HpAC50 and HpAC75 are cultured for 10 days under 5g / L sodium acetate and 7.5 g / L sodium acetate, and the survival rates of the domesticated algae species HpAC50 and HpAC75 reach 81.22% and 43.51% respectively and are increased by 422% and 460% compared with WT. The method is simple and convenient to operate, the tolerance of haematococcus pluvialis to acetate is remarkably enhanced, and a technical basis is provided for efficient production of astaxanthin and large-scale culture.

The invention discloses a haematococcus pluvialis culture illumination system based on edge control and a control method, and relates to the technical field of haematococcus pluvialis culture illumination control, and the haematococcus pluvialis culture illumination system comprises a plurality of sensor monitoring modules, a plurality of edge controllers, a plurality of illumination units and a digital twinborn monitoring platform. According to the method, the illumination intensity can be dynamically adjusted according to the actual growth state of algae, and the problem of algae growth inhibition or insufficient astaxanthin accumulation possibly caused in a traditional fixed illumination control mode is solved. All haematococcus pluvialis cultivation is divided into a plurality of areas for more precise control, an edge controller is arranged in each set area range, and the edge controllers can optimize illumination modes in the area ranges based on real-time data analysis. The digital twinborn monitoring platform establishes a three-dimensional digital model of haematococcus pluvialis growth by collecting data of the sensor monitoring module and the edge controller in real time, and real-time visual monitoring is achieved.

The invention relates to the field of functional food engineering, in particular to low-protein and high-energy baked food as well as a preparation method and application thereof. The low-protein and high-energy baked food is prepared from the following raw materials by mass: 15 to 25 g of sweet potato starch, 15 to 25 g of corn starch, 15 to 25 g of potato starch, 15 to 25 g of pea starch, 35 to 45 g of eggs, 4 to 6 g of corn oil, 8 to 12 g of linseed oil, 0.5 to 1.2 g of haematococcus pluvialis powder, 0.1 to 0.3 g of sodium ascorbate, 15 to 25 g of milk, 10 to 14 g of carrot juice, 18 to 24 g of tomato juice, 5 to 8 g of cane sugar and 5 to 8 g of a flavoring agent. The product disclosed by the invention is relatively low in cost, low in protein and high in energy, adopts different fillings to improve the taste, and has a health-care effect aiming at patients with nephropathy.

The application discloses a functional composition for improving bone and joint health, a conditioner and application. The functional composition comprises a first active component and a second active component; the first active component comprises elastin peptide, haematococcus pluvialis and ergothioneine; and the second active component comprises one or more of houttuynia cordata and non-denatured type II collagen. The application realizes the protection effect on joint, cartilage and bone health by scientific matching of the components and synergistically improving the glycosaminoglycan level in chondrocytes and the osteocalcin level in osteoblasts. Meanwhile, the components in the functional composition for improving bone and joint health are natural and have good biocompatibility, and have no obvious side effects, so that the application provides a kind of drug or a substitute for the treatment and prevention of bone diseases.

The invention discloses a treatment method of fermentation wastewater. The treatment method of the fermentation wastewater comprises the following steps: adding filamentous fungi and green algae into fermentation wastewater generated after fermentation treatment of kitchen garbage, carrying out fermentation culture, then carrying out solid-liquid separation, and extracting grease from the separated solid. The filamentous fungi comprise trichoderma harzianum, and the green algae comprise one or a combination of more selected from chlorella vulgaris, chlamydomonas, haematococcus pluvialis, hylothrix, scenedesmus obliquus and chaetoclada. According to the treatment method of the fermentation wastewater, grease can be produced while the fermentation wastewater is treated, and the treatment cost of the fermentation wastewater is reduced.

The present invention relates to the technical field of Haematococcus pluvialis cultivation, and specifically to a light and wind control system for a Haematococcus pluvialis cultivation base, which includes a base slope, support columns, and cultivation pipes. Wind control systems are spacedly arranged on the support columns, and a light control system is arranged between the left and right wind control systems. Auxiliary brackets are symmetrically arranged on the left and right between the same group of support columns. The light control system includes a main light structure, a secondary light structure arranged at the front end of the main light structure, and a retracting and deploying structure for driving the folding and unfolding of the secondary light structure. In this system, by setting the retracting and deploying structure, when the sunlight intensity is relatively high, it drives the light control system to descend to shade the cultivation pipes below, and at the same time fully opens the secondary solar panels, increasing the total light-receiving area and improving the total power generation when the sunlight conditions are good.

This invention discloses a multi-level astaxanthin complex, its preparation method, and its applications, relating to the fields of encapsulation technology or astaxanthin formulations. It includes a first encapsulation component containing astaxanthin and a second encapsulation component containing Haematococcus pluvialis isolated protein; optionally, the multi-level astaxanthin complex further contains cyclodextrin or its derivatives. This invention overcomes the problems of aggregation and uneven encapsulation caused by the direct addition of astaxanthin or astaxanthin solution and the need for vigorous stirring to promote the formation of encapsulated particles in existing astaxanthin complex preparation techniques through local microencapsulation in a foaming microenvironment.

The invention discloses a multifunctional special dietary food skin beautifying and shaping formula and application, and belongs to the technical field of beauty treatment. The multifunctional special dietary food at least comprises collagen peptide, collagen tripeptide, skipjack elastin peptide, carob bean extract, phosphatidylserine, casein hydrolytic peptide, nicotinamide, rice bran oil powder, haematococcus pluvialis, cranberry powder, oriental cherry flower powder, pig blood peptide powder, red date peptide powder and cattle spleen peptide. The health-care product can comprehensively supplement nutrition recovered after exercise, has the effects of beautifying, shaping and removing wrinkles, can effectively relieve pressure and regulate emotion, and also has the effects of improving female physiological health, strengthening the spleen and stomach and enhancing immunity.

The invention provides a production method of selenium-rich high-astaxanthin eggs. The production method comprises the following steps: (1) selection of laying hens: selecting healthy, disease-free and high-fecundity laying hens; (2) feed preparation: the feed comprises the following components in parts by weight: 5 parts of fish meal, 5 parts of shrimp shell meal, 10 parts of seaweed meal and 5 parts of vitamins; in addition, 0.0005 to 0.001 part of selenium yeast and 15 to 30 parts of haematococcus pluvialis are added; (3) illumination control: the illumination intensity of the henhouse is 10-20 lux; (4) temperature and humidity control: keeping the temperature of the henhouse at 13-20 DEG C and the humidity at 50-60%; (5) feeding management; and (6) collecting and disinfecting eggs. Through a reasonable feed formula, feeding management and illumination control, the quality and yield of eggs can be improved, the production cost is reduced, and market requirements are met. Meanwhile, the eggs produced by the method are rich in nutritional ingredients such as protein, fat, vitamins and mineral substances, and also have the health-care functions of resisting oxidation, enhancing immunity, protecting heart and blood vessels and the like.

The application discloses a kind of NADES and method for extracting astaxanthin from haematococcus pluvialis using it, wherein a kind of natural eutectic solvent for extracting astaxanthin from haematococcus pluvialis includes 1 molar part of hydrogen bond acceptor, 2-6 molar parts of hydrogen bond donor;The hydrogen bond acceptor is choline chloride, and the hydrogen bond donor is 1,4-butanediol or 1,2-propanediol.The extraction solvent of the application only needs to use the above-mentioned natural eutectic solvent to realize the extraction of astaxanthin in haematococcus pluvialis, which has the advantages of safety, green, higher extraction efficiency, etc., and is very beneficial to the industrialization and large-scale production of astaxanthin.

The application discloses a fermentation wastewater treatment method. The fermentation wastewater treatment method comprises the following steps: adding filamentous fungi and green algae into fermentation wastewater generated after fermentation treatment of kitchen garbage to carry out fermentation culture, then carrying out solid-liquid separation, and extracting oil from the separated solid. The filamentous fungi comprise Trichoderma harzianum, and the green algae comprise one or more selected from the group consisting of Chlorella vulgaris, Chlorella sp., Haematococcus pluvialis, Leptolyngbya sp., Agmenellum sp. and Comatulicium sp. The fermentation wastewater treatment method can produce oil while treating fermentation wastewater, and reduces the cost of fermentation wastewater treatment.

The invention discloses a functional composition capable of improving bone and joint health, a conditioner and application. The functional composition comprises a first active component and a second active component, the first active component is prepared from elastin peptide, haematococcus pluvialis and ergothioneine; the second active component is prepared from one or more of herba houttuyniae and non-denatured type II collagen. Through scientific compatibility of all the components, the levels of glycosaminoglycan in chondrocytes and osteocalcin in osteoblasts are synergistically improved, and the protection effect on joint, cartilage and skeleton health is achieved. Meanwhile, a plurality of components in the functional composition capable of improving the health of the bones and the joints are natural in source, good in biocompatibility and free of obvious side effects, and a medicine or an alternative scheme thereof is provided for treatment and prevention of the bone diseases.

The invention provides a haematococcus pluvialis freeze-dried algae seed production process which comprises the following steps: algae seed acquisition: automatically separating haematococcus pluvialis seeds from a natural water body water sample by adopting a flow cytometry sorting technology in combination with a fluorescence labeling method; expanding cultivation: adopting an open autotrophy mode, and automatically adjusting the illumination intensity and duration by utilizing an intelligent control system; concentrating, namely concentrating the algae liquid into algae mud through a centrifugal machine; compounding and blending, namely adding a freeze-drying protective agent into the concentrated algae seeds, and uniformly mixing; and filling and storing, namely packaging by adopting nitrogen, and storing in a shady and dry place for a long time. According to the method, flow cytometry sorting is combined with a fluorescence labeling method to separate algae species, the expanding culture environment is intelligently regulated and controlled, a freeze-drying protective agent formula and a vacuum freezing nitrogen packaging process are optimized, the dormant state and long-term stable storage of the algae species are achieved, the storage stability and transportation convenience of the algae species are improved, and meanwhile it is guaranteed that the algae species can be revived with water; and adding a culture medium for propagation and propagation.

The present invention provides a composition, a preparation method and an application for improving male reproductive ability, belonging to the field of bioengineering technology. It is prepared from the following raw materials by weight: 10-30 parts of Bacillus subtilis fibrinolytic enzyme, 5-15 parts of Haematococcus pluvialis, 3-10 parts of walnut kernel extract, 3-10 parts of Cordyceps militaris extract, and 0.3-0.5 parts of rice bran alkanol; the Bacillus subtilis fibrinolytic enzyme is prepared by fermenting the strain Bacillus subtilis QK02, and the strain is preserved in the China Center for Type Culture Collection with the preservation number CCTCC NO: M 203078. The present invention provides a Bacillus subtilis fibrinolytic enzyme composition with the efficacy of improving male reproductive ability, rationally compatible with medicinal and edible ingredients, having a significant synergistic effect, promoting human blood circulation and metabolism, eliminating fatigue, supplementing androgen, and significantly improving human immunity.