565 results about "Haematococcus pluvialis" patented technology

The invention discloses a process for extraction of astaxanthin in Haematococcus pluvialis. According to the process, wall-broken Haematococcus pluvialis is obtained by using an acid method, and then an astaxanthin extract is obtained through ethanol dehydration and organic solvent extraction. The wall breaking and extraction process provided by the invention has the advantages of easiness, high efficiency, convenient operation, low energy consumption and controllable quality, and the wall breaking rate and the extraction ratio of Haematococcus pluvialis can reach more than 95%. The astaxanthin extract prepared by using the process is a dark red oily viscous substance and can meet requirements for raw materials needed in production of health food, cosmetics and medicines.

The invention discloses a set of apparatus and a method for culturing Haematococcus pluvialis and converting astaxanthin in large scale. The apparatus consists of a photo-bioreactor system set on the frisket, aeration equipment, culture fluid inoculation equipment and still cell harvesting equipment. Floating or sunken pulse equipment is set on the frisket. The apparatus don't need establishment of artificial culture pool and other institution, but uses natural water body to culture Haematococcus pluvialis in large scale. It is low cost, the culture temperature, illumination and water flow rate is stable, culture fluid flows with each other, aeration is continuous, culture conditions are precedent, which make the growth and increment of Haematococcus pluvialis almost at equal pace. The growth rate of Haematococcus pluvialis can reach to 25%, biomass increase is fast, doubles 3-5 days. The photo-bioreactor can be hoisted into required depth of the water, which solves the contradiction of difference of temperature, illumination and nutrition of Haematococcus pluvialis culture and astaxanthin conversion.

The invention provides a method for extracting astaxanthin from haematococcus pluvialis. Astaxanthin is extracted by adopting above-critical CO2 extracting process. The temperature of an extracting kettle is ranged from 30 to 50 DEG C and the pressure is ranged from 20 to 30 MPa. Flowing speed of CO2 fluid is 20 to 40 L/h and the extracting time is 2 to 4 h. The astaxanthin has strongest inoxidizability and is easy to be oxidized. The extracting method of the invention is simple, rapid and convenient to operate. CO2 is used as the extraction solvent in extraction process, so the production process is environment-friendly and the extraction has no organic solvent residue. The temperature during extraction process is low for efficiently avoiding active substance to decompose at high temperature. The acquired astaxanthin extraction is bolarious pasty substance and can be used as materials for developing health-care food or officinal component.

The invention provides a haematococcus pluvialis culture production method and a method for further producing astaxanthin. The haematococcus pluvialis culture process is carried out in a large-size container, and haematococcus pluvialis is cultured under the irradiation of an artificial LED (light-emitting diode) light source; the artificial LED light source is provided by a high-brightness LED, the wavelength range of single-color light emitted by the LED light source is from 450nm (width band of 30nm) to 640nm (width band of 30nm); the used illumination intensity is 30mu E/m<2>.s-3000mu E/m<2>.s; in the culture process, cells are maintained to be suspended in the culture liquid in utilizing a ventilation mode, and temperature is maintained to be 15-28 DEG C; and the pH value is maintained to be 6.8-8.5. According to the invention, on one hand, more nutrient substances are accumulated so as to accelerate growth and reproduction; on the other hand, the problem that the temperature is difficult to control due to the adoption of a natural sun light source is solved, thereby saving a large amount of energy consumed for controlling the temperature in summer and winter, reducing production cost and environmental pollution; and simultaneously, according to the characteristic that photosynthesis pigments demand different light qualities, the most effective single-color diode light source is adopted, thereby improving energy utilization rate, production efficiency and cell concentration.

Provided are natural forage yolk toner produced by orange peels and flavedo and a preparation method of the natural forage yolk toner. The raw materials of the natural forage yolk toner comprise, by weight, 43-45 parts of orange peel powder, 37-39 parts of flavedo powder, 11-13 parts of haematococcus pluvialis powder, 5-7 parts of chaetoceros curvisetus powder and 6-8 parts of addition agents, and the addition agents comprise solid hydroxypropyl-beta-cyclodextrin and kaolin with the weight ratio of 19:1. The orange peels, the flavedo, haematococcus pluvialis and chaetoceros curvisetus are dried in the sun or baked dry, the water content is 10 percent to 13 percent, and the orange peels, the flavedo, the haematococcus pluvialis and the chaetoceros curvisetus are respectively smashed into middlings of 60 meshes to 80 meshes. The middlings of the raw materials are mixed according to the ratio and are mixed with the addition agents. The mixture is treated for 15 min to 30 min through a high-energy vibration mill after being mixed evenly, a solid state mechanical chemical reaction happens to the raw materials, and the finished product is obtained. The natural forage yolk toner and the preparation method are simple in production process, convenient to use and obvious in effect, the color value of a yolk is 74 percent higher than that of a control group, meanwhile, the laying rate and average egg weight can be improved, circular economy and sustainable development are achieved, economic benefits are brought for enterprises, and environmental pressure is relieved.

The invention discloses a method for preparing an astaxanthin extractive from haematococcus pluvialis powder. The method comprises the following steps: (1), weighing haematococcus pluvialis powder, adding food-grade alcohol, fully and uniformly mixing to obtain a mixture as a reaction system, and extracting the reaction system for 1 to 2 hours at a room temperature in a dark place, wherein the ratio of haematococcus pluvialis powder to food-grade alcohol is 1 g/10-20 mL, and the food-grade alcohol adopts a food-grade alcohol solution with the volume concentration of larger than or equal to 95%; (2), centrifuging after extraction is finished to respectively obtain residue and supernatant liquor; (3) replacing the haematococcus pluvialis powder obtained from the step (1) with the residue, and repeating the step (1) and the step (2) at least once in sequence; and (4), combining the supernatant liquor obtained from the step (2) and the supernatant liquor obtained from the step (3), and recovering alcohol through vacuum concentration to obtain a dark red extract, namely the astaxanthin extractive. The astaxanthin extractive, prepared by the method, has the characteristic of high content of the astaxanthin monomer.The invention discloses a method for preparing an astaxanthin extractive from haematococcus pluvialis powder. The method comprises the following steps: (1), weighing haematococcus pluvialis powder, adding food-grade alcohol, fully and uniformly mixing to obtain a mixture as a reaction system, and extracting the reaction system for 1 to 2 hours at a room temperature in a dark place, wherein the ratio of haematococcus pluvialis powder to food-grade alcohol is 1 g/10-20 mL, and the food-grade alcohol adopts a food-grade alcohol solution with the volume concentration of larger than or equal to 95%; (2), centrifuging after extraction is finished to respectively obtain residue and supernatant liquor; (3) replacing the haematococcus pluvialis powder obtained from the step (1) with the residue, and repeating the step (1) and the step (2) at least once in sequence; and (4), combining the supernatant liquor obtained from the step (2) and the supernatant liquor obtained from the step (3), and recovering alcohol through vacuum concentration to obtain a dark red extract, namely the astaxanthin extractive. The astaxanthin extractive, prepared by the method, has the characteristic of high content of the astaxanthin monomer.

The invention provides a method for extracting astaxanthin from haematococcus pluvialis, and belongs to the technical fields of bioprocess technology and chemical technology. The method comprises the following steps: completing the selection of raw materials through abnormal growth of cells of the selected haematococcus pluvialis, dilution of high-density algae cells and light induction, carrying out wall-breaking treatment on frond by using an ultrahigh pressure extractive technology, then extracting, eluting, concentrating, saponifying and centrifuging, thus obtaining the astaxanthin. The method has the advantages that the consumption of an organic solvent is little, an extraction agent can be circularly utilized, the environmental pollution is little, the production cost is low, the recovery rate is high, few impurities exist, the possibility of oxidative degradation of the astaxanthin is reduced, and the extraction quality of the astaxanthin is improved.

The present invention relates, in general, to a biotechnological method for production of (3S,3'S) astaxanthin. In particular, the present invention relates to a peptide having a beta-C-4-oxygenase activity; a DNA segment coding for this peptide; an RNA segments coding for this peptide; a recombinant DNA molecule comprising a vector and the DNA segment; a host cell or organism containing the above described recombinant DNA molecule or DNA segment; and to a method of biotechnologically producing (3S,3'S) astaxanthin or a food additive containing (3S,3'S) astaxanthin, using the host.

The invention discloses a method for producing astaxanthin by haematococcus pluvialis. The method comprises the steps of: firstly, naturally culturing the haematococcus pluvialis in water of a culture pool for 6-8 days so that the haematococcus pluvialis proliferates, then adding potassium dihydrogen phosphate and sodium nitrate to the pool water, and covering the pool with a red thin film, so that algae cells of the haematococcus pluvialis rapidly grow to reach conversion state under the irradiation of 630nm-760nm light waves; transferring the algae cells at the conversion state to pool water with potassium dihydrogen phosphate, the final concentration of which is 0.02-0.05g/L, covering the pool with a blue thin film, so that the algae cells of haematococcus pluvialis are cultivated to mature under the irradiation of 430nm-490nm light waves, and thus obtaining haematococcus pluvialis, the algae cells of which are rich in astaxanthin, wherein potassium dihydrogen phosphate, sodium nitrate and red lights can enable the algae cells to rapidly grow and reach conversion state in shorter time, and the potassium dihydrogen phosphate and blue lights can enable the algae cells to rapidly grow, mature and accumulate astaxanthin. The method has the advantages of little investment, cultivation simpleness and high economic benefits.

The invention discloses a moisturizing skin care composition containing a haematococcus pluvialis extractive, the composition comprises, by weight, 0.1-10 parts of haematococcus pluvialis extractive, 0.1-5 parts of Ectoin, 0.01-0.5 part of EDTA disodium, 1-180 parts of first moisturizer and 0.1-36 parts of second moisturizer. The invention further provides an alga moisturizing mask liquid and a preparation method thereof. Compared with the prior art, the mask liquid is rich in the ingredients of astaxanthin, beta glucan, hyaluronic acid and the like, has high moisturizing efficacy, can lock water continuously, is free of sticky and greasy feels, further contains the Ectoin, can realize relieving and moisturizing, and defends damage of outside stimulation to the skin.

The invention discloses a method for producing astaxanthin by inducing Haematococcus pluvialis, which is characterized by comprising the following steps: in the vegetative growth phase, inoculating Haematococcus pluvialis in a nutrient culture medium, and culturing; continuously keeping irradiation on the Haematococcus pluvialis and ensuring that the intensity of illumination arriving at the Haematococcus pluvialis cell surface is 20-200 mu mol.m<-2>.s<-1>; in the induction stage, adding ethanol and seawater crystal into the production culture medium, wherein the ethanol added into the production culture medium accounts for 0.5-3 vol%, and the seawater crystal added into the production culture medium accounts for 0.5-1 wt%; and continuously introducing filtered air mixed with 1-3 vol% carbon dioxide into the production culture medium, wherein 0.2-0.4L/minute of the air is introduced to every 1L of production culture medium. The method can induce the Haematococcus pluvialis to quickly accumulate the astaxanthin, is simple and has the advantages of high yield and favorable effect.

The invention provides a new method for preparing an astaxanthin monomer. In the method, a free astaxanthin monomer is obtained by hydrolyzing astaxanthin ester with lipase. The recovery rate of the astaxanthin monomer reaches 63.2 percent by using the method. The method is simple to operate, namely a desired product can be obtained by one reaction and extraction; and the method saves the energy consumption and can avoid the loss of the astaxanthin during long-term reaction to a certain extent. The method lays the foundation of the industrial application for extracting the astaxanthin monomer from crude extract of haematococcus pluvialis by an enzyme method.

The invention discloses an alga ferment healthcare solid drink. The alga ferment healthcare solid drink is prepared from the following raw materials in percentage by weight: 5-13% of spirulina platensis, 8-10% of spirulina maxima, 5-15% of chlorella pyrenoidosa powder, 10-12% of haematococcus pluvialis, 10-13% of euglenophyta, 10% of alpha-amylase, 5% of lipase, 10% of cellulase, 5% of protease and 15% of fructo-oligosaccharides. A preparation process of the alga ferment healthcare solid drink comprises the following steps: (1) selecting and baking the materials; (2) drying; (3) adding the fructo-oligosaccharides; (4) crushing, purifying and sterilizing; (5) mixing; (6) carrying out incense enhancing and equalization treatment; (7) packaging. Compared with the prior art, the alga ferment healthcare solid drink has the advantages that natural raw materials serve as effective ingredients, and the intake and absorption of villi are controlled in the intestinal tract; the metabolic balance of the intestinal tract is regulated, and the value of uric acid in blood can be more effectively reduced; gout is prevented and improved, the propagation of beneficial bacteria of the intestinal tract is improved, the breeding of miscellaneous bacteria of the intestinal tract is inhibited, and the content of oxygen in the blood is increased; the immune system is regulated, and the immunity is enhanced.

The invention relates to an artificial particle feed for juvenile turbot. The artificial particle feed is characterized by being prepared from whitefish powder, euphausiid powder, scallop powder, beer yeast, starch, cod liver oil, yolk powder, whey powder, L-alanyl-L-glutamine, choline chloride, soybean lecithin, schizochytrium limacinum powder, haematococcus pluvialis powder, compound vitamins, compound mineral substances, Chinese herbal medicines and sodium alginate. As the various raw materials are added into the feed, the comprehensiveness of nutrition is guaranteed. An immunopotentiator is added into the feed, so that the non-specificity immunity of fish bodies is enhanced. The feed is green and pollution-free. The schizochytrium limacinum powder and the haematococcus pluvialis powder are added into the feed, and the content of vitamin E is improved, so that the whitening rate of the juvenile fish is effectively reduced. The Chinese herbal medicine components are added, so that the immunity of the juvenile fish can be improved and parasite diseases in a juvenile fish growing phase are prevented from happening.

The invention discloses a health-care food for resisting aging and enhancing immunity and a preparation method of the health-care food. The health-care food comprises the following components by mass percent: 20-40 percent of broken-wall haematococcus pluvialis powder, 15-30 percent of broken-wall pine pollen, 10-20 percent of spirulina powder, 5-10 percent of ultramicro maca powder, 10-15 percent of ultramicro American ginseng powder, 6-10 percent of ultramicro moringa seed powder and 3-5 percent of panax notoginseng saponins. The health-care food for resisting aging and enhancing immunity is prepared by the following steps: mixing the broken-wall haematococcus pluvialis powder, the broken-wall pine pollen and the spirulina powder to obtain mixed powder; subjecting the mixed powder to enzymolysis to obtain an enzymolysis product; and adding the panax notoginseng saponins, the ultramicro maca powder, the ultramicro American ginseng powder and the ultramicro moringa seed powder to the obtained enzymolysis product, and mixing the obtained mixture. The formula of the health-care food provided in the invention is reasonable, is safe and reliable, has a remarkable effect and has an excellent synergistic effect in resisting aging and enhancing immunity. The health-care food provided in the invention has the efficacy of resisting oxidation, enhancing immunity, supplementing various nutritious substances, tonifying qi, nourishing yin, promoting the secretion of saliva or body fluid, relieving restlessness, invigorating the circulation of blood, reducing blood pressure and blood fat, and the like.

A medicine delivery system includes an inner capsule containing carotenoids and an outer capsule in which the inner capsule is contained within the outer capsule and the outer capsule containing a therapeutically effective amount of krill oil. In one example, the carotenoids comprise at least S, S′-astaxanthin derived from Haematococcus pluvialis, and one or more of lutein and/or trans-zeaxanthin or meso-zeaxanthin. The medicine delivery system also includes 0.5 to 8 mg of astaxanthin, 2 to 15 mg of lutein and 0.2 to 12 mg of trans-zeaxanthin contained within the inner capsule. In a specific example, the medicine delivery system includes about 4 mg of astaxanthin, about 10 mg of lutein and about 1.2 mg of trans-zeaxanthin contained within the inner capsule.

The invention discloses health food with an anti-oxidization function. The health food is characterized by being prepared from the following components in parts by mass: 5-8 parts of haematococcus pluvialis powder, 1-3 parts of krill oil, 0.003-0.01 part of natural VE, preferably, 7 parts of haematococcus pluvialis powder, 2 parts of krill oil, 0.006 part of natural VE. The health food has the beneficial effects that the haematococcus pluvialis powder and the krill oil are combined according to a certain proportion, and are supplemented with the natural VE to achieve a good synergistic effect and a good anti-oxidation effect; moreover, various effects of improving body immunity, delaying skin ageing, protecting eyes and central nervous system health, preventing cardiovascular diseases, resisting fatigue, protecting nerve cells, relieving gout and rheumatoid arthritis, and the like of various substances can be achieved, and comprehensive nutrient substances can be supplemented for the human body.

The invention relates to a method for achieving high yield of astaxanthin through haematococcus pluvialis. The method comprises the steps that 1, stable-period cultivation is conducted; 2, amplifying cultivation of algal cells is conducted till the cell density does not rise any more; 3, stress cultivation is conducted and used for accumulation of haematococcus pluvialis astaxanthin, the cultivation temperature ranges from 18 DEG C to 33 DEG C, illumination adopts unblocked sunlight, and the sunlight intensity is 10,000 Lux or above. According to the method for achieving the high yield of the astaxanthin, a BBM cultivation medium which has the same salinity and is lack of nitrogen, phosphate and sulfur is used under the high light condition for stress cultivation, the yield of the astaxanthin can be increased to the maximum extent, a double-layer filter cloth is used for filtration, insect attacks introduced in the cultivation process can be removed, and harvesting of haematococcus pluvialis cells at the last period of cultivation is convenient.

The invention provides a method for producing astaxanthin by haematococcus pluvialis induced by methyl jasmonate, which comprises the following steps: (1) culturing methyl jasmonate cells to exponential phase so as to obtain cultured microalgal liquid for induction; and (2) accumulation of astaxanthin: adding methyl jasmonate concentrate the mass concentration of which is 95 percent into cultured microalgal liquid until the final concentration of the methyl jasmonate is 4.95 to 5.05 mg/L; then culturing the microalgal liquid added with methyl jasmonate until the haematococcus pluvialis cells are completely red under an optical microscope at a temperature of 25 to 28 DEG C and an optical intensity of 4500 to 5000lx and under the conditions of continuous illumination of fluorescent lamps and nutritive salt starved compound stresses so as to finish the accumulation of astaxanthin, wherein at the moment of finishing the accumulation of astaxanthin, the maximum output of the astaxanthin is reached. The invention is simple, easy to operate and low in cost, can greatly shorten the time that the haematococcus pluvialis cells are completely red, and significantly increase the output of the astaxanthin, thereby greatly improving the efficiency of producing astaxanthin by haematococcus pluvialis.

The invention belongs to the technical field of biological products, and relates to a preparation method for astaxanthin microcapsules. The specific process is as follows: uniformly stirring pure gum, maltodextrin and distilled water to prepare wall material solution at first, and then mixing and stirring the wall material solution with a compound emulsifier until the wall material solution is completely emulsified to obtain wall material emulsifier solution; then carrying out a wall-breaking treatment on haematococcus pluvialis spores and then preparing astaxanthin solution by a conventional extraction process, and mixing the astaxanthin solution with vitamin E to obtain astaxanthin oil solution; then mixing and stirring the wall material emulsifier solution with the astaxanthin oil solution until an emulsifier is formed, then homogenizing by a super cell homogenizer, adjusting the temperature of the emulsifier, and then homogenizing by the super cell homogenizer until water-colour oil microcapsules are formed; finally carrying out spray-drying on the water-colour oil microcapsules by a spray dryer until a wall material is condensed, so as to prepare the astaxanthin microcapsules. The preparation method is simple in process, high in product activity, good in application effect, stable in performance, free from the influence of external environment, and high in utilization rate.

The invention provides a method for quickly producing astaxanthin from haematococcus pluvialis stimulated by brassinosteroids. The method comprises the following steps: (1) liquid preparation: culturing haematococcus pluvialis cells to an exponential phase to obtain of inductive cultured liquid; and (2) astaxanthin accumulation: dissolving the brassinosteroids with absolute ethanol of double volume, and preparing 1mg/mL brassinosteroids mother solution with dimethylsulfoxide, and adding the brassinosteroids mother solution to the cultured liquid until the final concentration of the brassinosteroids is between 4.95 and 5.05mg/L; and culturing the brassinosteroids-containing liquid under composite stress conditions of temperature of between 25 and 28 DEG C, light intensity of between 4,500 and 5,000lx, continuous lighting of fluorescent lamp and nutrient hunger until the haematococcus pluvialis cells completely turn red under observation of an optical microscope, and yield of the astaxanthin reaches maximum. The method has the advantages of simpleness and easy operation and low cost, and can greatly shorten the time that the haematococcus pluvialis cells completely turn red, so the efficiency for producing the astaxanthin from the haematococcus pluvialis cells is greatly improved.

The invention belongs to the technical field of medicines, and relates to a method for extracting astaxanthin from haematococcus pluvialis. The method comprises the steps of culturing a seed stock solution in a glass apparatus with a drainage system, and carrying out later amplification culture of the haematococcus pluvialis and accumulation culture of the astaxanthin after 10-15 d; centrifuging a culture in an exponential growth period of seed culture; and inoculating cell clusters in a BBM basal medium to obtain a primary culture. The later accumulation culture of the astaxanthin is amplification culture by using a breathable plastic bag type simple device provided by the invention. In the accumulation stage, a stress culturing method is adopted to obtain a lab-scale test haematococcus pluvialis culture; and haematococcus pluvialis powder is obtained by spray drying. According to a preparation technology that extracts astaxanthin from the haematococcus pluvialis by adopting an ultrasonic cell disruption assisted mixed solvent extraction method, the haematococcus pluvialis powder is added in an organic solvent to carry out ultrasonic cell disruption, and then the astaxanthin is obtained by the steps of reflux extraction in a water bath, suction filtration, filtrate merging and concentration. Compared with a conventional direct extraction method, the method provided by the invention saves extraction time, and increases astaxanthin yield.

The invention relates to the technical field of health care food, in particular to a krill oil microcapsule with an antioxidation effect and a preparation process thereof. Antarctic krill oil, DHA algal oil and haematococcus pluvialis are used as core materials; a proper amount carrageenan is added into an oil phase; chitosan oligosaccharide and gamma-cyclodextrin are used as wall materials, and are proportionally mixed with an oil phase antioxidant, a water phase antioxidant, an emulsifying agent, a dispersing agent and the like to be prepared into microemulsion; through freeze drying, powder grinding, sieving and the like, the krill oil microcapsule is prepared. The krill oil microcapsule obtained by the process has the advantages that the stability of the krill oil on the factors such as light, temperature and humidity is improved; the content of bioactive substances such as astaxanthin and DHA in the microcapsule is high; the antioxidation performance of the krill oil is greatly improved.