49results about How to "Increased astaxanthin content" patented technology

The invention relates to a method for increasing biomass and astaxanthin content of haematococcus pluvialis, and belongs to the technical field of bioengineering. The method comprises steps as follows: a BBM culture medium with sodium acetate or magnesium acetate added is prepared firstly, after being sterilized, the BBM culture medium is inoculated with the haematococcus pluvialis cultured to theexponential phase until the concentration reaches 2.0*10<5>-2.5*10<5> cells / mL, culturing is performed at the temperature of 24-26 DEG C under the condition of light intensity being 2000-2500 lx, andsampling is performed every other day for biomass measurement; algae cells are collected when the biomass is maximum, diluted by the nitrogen-deficient BBM culture medium with melatonin added until the concentration is 2.0*10<5>-2.5*10<5> cells / mL, culturing is performed at the temperature of 26-28 DEG C under the condition of light intensity being 12000-14000 lx, and sampling is performed everyother day for astaxanthin content measurement. The method is simple and easy to operate, growth cycle of the haematococcus pluvialis can be shortened to a certain degree, and the biomass and the astaxanthin content of the haematococcus pluvialis are increased.

The invention discloses a method for increasing the natural astaxanthin content of eggs. The feed for increasing the natural astaxanthin content of eggs is composed of basic feed and a premixed material mixed with natural astaxanthin, the premixed material is formed by mixing natural haematococcus pluvialis, haematococcus pluvialis oil, feed-grade cuttlefish paste or shrimp paste, spiral seaweed powder and edible lactic acid bacteria, and the basic feed is formed by mixing corn, bran, soybean cake, fish oil, salt and methionine. According to the method, natural haematococcus pluvialis and haematococcus pluvialis oil are added to traditional chick feed, the proportion of esterified astaxanthin in the eggs is increased, the loss amount of astaxanthin in the egg cooking process is reduced, the feed favor is improved by adding the cuttlefish paste or shrimp paste, spiral seaweed powder and edible lactic acid bacteria, the food digestibility and biological value are increased, the astaxanthin content in the eggs is greatly increased, the growth environment is controlled, and astaxanthin accumulation is promoted.

The invention discloses a method for increasing the content of astaxanthin in haematococcus pluvialis. The method comprises the step of adding monoethanolamine (MEA) into a culture solution at an immobile stage of culture of haematococcus pluvialis until the final concentration is 50-400 mg / L. The method for increasing the content of astaxanthin in haematococcus pluvialis is a new technical routefor increasing the content of astaxanthin in haematococcus pluvialis, the content of astaxanthin in cells is significantly increased, the culture time is shortened, and the culture efficiency is improved.

The invention discloses a method of utilizing seawater to facilitate haematococcus pluvialis to produce natural astaxanthin, and relates to the technical field of artificial cultivation of haematococcus pluvialis. The method comprises the following steps: utilizing seawater to prepare an improved culture medium of haematococcus pluvialis, adding seawater step by step to induce vegetative growth of haematococcus, cell type transformation and accumulation of astaxanthin. The method can be applied to cultivate haematococcus pluvialis at large scale at the coastal region rich in the seawater resource to produce the natural astaxanthin; compared with the conventional cultivation method of using freshwater, the method has the advantages of short production cycle, high astaxanthin content, and low material cost, and can achieve a wide industrialized application prospect.

The invention discloses a natural pigment feed additive for freshwater aquaculture of vannamei. The natural pigment feed additive for the freshwater aquaculture of the vannamei comprises the following components in parts by weight: 5-9 parts of dunaliella salina powder, 5-7 parts of carrot powder, 1-8 parts of spirulina powder and 22-38 parts of pigment stabilizer. 0.1-2% of the natural pigment feed additive for the freshwater aquaculture of the vannamei by mass is added into feed for the freshwater aquaculture of the vannamei, so that the astaxanthin content in the body of the adult vannamei can be obviously improved to improve the color, and the non-specific immunity of the vannamei can be improved to reduce diseases and further the economic benefit is improved.

The cultivating and breeding method of marigold features the direct field seeding and transplanting. The new variety of adonis amurensis obtained with wild adonis amurensis and through domestication, scientific management and optimizing breeding. The obtained adonis amurensis has synchronous blooming, strong pest and disease resistance, high astaxanthin content and other advantages, and is suitable for commercial planting in large area.

The invention discloses a biological reaction kettle and a method for promoting proliferation and reddening of haematococcus pluvialis. The biological reaction kettle comprises a main body reaction kettle, wherein the main body reaction kettle is provided with a pH (potential of hydrogen) sensing head interface; the pH sensing head interface is connected with a pH controller; one end of the pH controller is connected with an industrial carbon dioxide bottle, and the other end of the pH controller is connected with the main body reaction kettle through an air intake adjusting valve; a filter is arranged at an outlet of the industrial carbon dioxide bottle; an LED (light emitting diode) lamp tube is arranged in the main body reaction kettle, and is connected with an LED driving controller; gas explosion stone is arranged at the bottom wall of the inner side of the main body reaction kettle, and is connected with a T-level filter through the air intake adjusting valve; the T-level filter is connected with an industrial air compressor; the LED lamp tube is connected with one end of a water cooling machine through the air intake adjusting valve, and the other end of the water cooling machine is connected with the LED lamp tube. The biological reaction kettle has the advantages that the problem of adverse influence by weather, light ray, pollution, environment temperature and the like in the existing traditional microalgae culture type is solved, and the growth speed of microalgae is greatly improved.

The present invention discloses a production process for extracting natural astaxanthin by utilizing supercritical fluid CO2. It is characterized by that it utilizes natural plant adonis chrysocyathus, places it in a supercritical fluid extraction equipment and adopts the processes of heating, boosting and injecting CO2 fluid so as to obtain natural astaxanthin.

The present invention relates to a novel method for producing astaxanthin by using microalgae. The method comprises: heterotrophic cultivation of microalgae, dilution, photo-induction, collection of microalgal cells, and extraction of astaxanthin. The method according to the present invention takes full advantages of rapid growth rate in the heterotrophic stage and fast accumulation of astaxanthin in the photo-induction stage by using a large amount of microalgal cells obtained in the heterotrophic cultivation stage, so as to greatly improve the astaxanthin production rate and thereby achieve low cost, high efficiency, large scale production of astaxanthin by using microalgae. The method not only provides an important technical means to address the large scale industrial production of astaxanthin through microalgae but also ensures an ample source of raw material for the widespread utilization of astaxanthin.

The invention discloses a fermentation culture medium and method for producing astaxanthin through fermentation of Phaffia rhodozyma. Each 1L of water in the fermentation culture medium contains the following components by weight: 80g-140g of glucose, 5g-8g of yeast extract powder, 5g-8g of malt extract powder, 6g-10g of proteins, 5g-9g of ammonium sulfate, 0.5g-2.5g of magnesium sulfate, 10g-30gof tomato powder and 0.2%-0.5% (volume fraction) of absolute ethyl alcohol. The invention discloses the culture medium and method for highly producing astaxanthin by virtue of Phaffia rhodozyma. By carrying out fermentation through the method, the biomass of thalli and the content of astaxanthin are high, the oxidation resistance of astaxanthin is strong and is equal to 550 times of that of vitamin E and 10 times of that of beta-carotene. Astaxanthin has physiological effects of enhancing the immunity, inhibiting tumors, eliminating in-vivo free radicals and the like and has wide application prospects in health products, medicines, cosmetics and the like.

The invention discloses a selenium-rich chlorella based functional fish feed and a preparation method of the selenium-rich chlorella based functional fish feed, the selenium-rich chlorella based functional fish feed comprises a rapeseed dreg, a rice bran, a flour, a fish meal, a soybean meal, a compound mineral substance, multi-vitamins, monocalcium phosphate, choline chloride, and a selenium-richchlorella powder in the formula, and the selenium-rich chlorella powder is prepared by adding 2 mg / L sodium selenite into a BG-11 culture medium under the optimized condition, culturing, finally homogenizing and breaking a wall after enzymolysis. The high selenium accumulating capability and the organic selenium transforming capability of the chlorella are used, the selenium content of the feed and the proportion of the organic selenium are effectively increased, the safety and the bioavailability of the selenium are strengthened, the economic benefit is further increased while the physique of fish and the meat quality are improved, the increase of the selenium content of the culture medium also promotes the synthesis of astaxanthin of the chlorella, and the immunity of a fish body is improved. The organic selenium-rich fish provides the technical support for the industrial development of selenium-rich fish foods on strengthening the immunity of the human body and resisting aging andcancers.

The invention relates to a ship-borne Antarctic krill powdering process. The process comprises the following steps: (1) material preparation: feeding Antarctic krill into a feeding pump, (2) scraper heater heating and digester heating, (3) material separation, (4) material drying, (5) material cooling, (6) material crushing, (7) liquid treatment, and (8) wastewater treatment. By adopting the production process of the invention, the yield of the product is obviously improved, the astaxanthin content and the protein content of the product are also obviously improved, and the quality of the product is significantly higher than that of shrimp powder produced by traditional methods. After the wastewater is evaporated, all resources in the entire process are fully utilized without any waste of resources.

The invention discloses a method for producing astaxanthin by utilizing phaffia rhodozyma. The method comprises the following steps: 1) grinding and crushing shrimp offal to obtain milled slurry, boiling and cooling the milled slurry, adding compound protease and papain to perform enzymolysis so as to obtain enzymatic hydrolysate; adding straw cellulose dry matters, ammonium sulfate and molasses into the enzymatic hydrolysate, uniformly mixing, and autoclaving to obtain a fermentation matrix; 2) activating phaffia rhodozyma; 3) inoculating the activated phaffia rhodozyma into an expanded medium, culturing under stirred conditions for 45-50 hours, mixing the fermentation matrix, introducing sterile oxygen for continuously culturing for 45-50 hours, introducing mixed gas composed of sterilecarbon dioxide, oxygen and ethylene, wherein the volume ratio of the carbon dioxide to oxygen to ethylene is 2:1:0.03, and the ventilatory capacity is 0.2V / (V.min), and continuously culturing for 45-50 hours so as to obtain the phaffia rhodozyma; and 4) extracting and purifying, thereby obtaining the astaxanthin. The method disclosed by the invention has the characteristics of low production cost,high benefits and the like.

The invention belongs to the field of preparation of microcapsule preparations, and particularly relates to a microcapsule preparation rich in astaxanthin as well as a preparation method and application of the microcapsule preparation. The microcapsule comprises a capsule wall material and a core material which is wrapped by the capsule wall material and contains astaxanthin. The microcapsule preparation rich in astaxanthin is prepared by combining molecular characteristics of astaxanthin, constructing a series of deep eutectic solvent systems, dissolving astaxanthin at a molecular level to serve as an oil phase, and emulsifying the oil phase and a water phase containing a particle stabilizer under the action of external force. The astaxanthin exists in the microcapsule in a molecular state dissolving form, the embedding rate is high, the storage stability of the natural astaxanthin can be remarkably improved, the fluidity is good, and the in-vivo absorption rate of the natural astaxanthin can be remarkably improved. The microcapsule disclosed by the invention can be applied to products such as foods, beverages, cosmetics, skin care products, medicines and the like.

The invention relates to a method for static and dynamic synergistic extraction of astaxanthin by wall-broken lactobacillus supercritical CO2. The method comprises the following steps: adding the dried plant lactobacillus to ethanol, and after ball-milling wall-breaking, obtaining the lactobacillus bacteria slurry as an extraction raw material; mixing diatomite and lactobacillus bacteria slurry, and adding a mixture to an extraction vessel; setting the supercritical extraction temperature to 35-60 DEG C and the supercritical extraction pressure to 10-40 MPa, and flowing supercritical CO2 fromabove the extraction vessel, when the supercritical extraction temperature and the supercritical extraction pressure reach a set value and are stabilized, closing a valve (V2), and cutting off the extraction kettle and a separation vessel; and after the high-pressure solubilization static extraction is completed, regulating the valve and introducing the supercritical CO2 for dynamic synergistic extraction. The method is used for extracting astaxanthin from lactobacillus, has the advantages of low cost, simple operation steps, and high extraction rate, no other organic solvent consumption except ethanol is required, and the whole process is green, low energy consumption and suitable for commercial application.

The invention discloses a method for producing astaxanthin by using haematococcus pluvialis. The method comprises the following steps that the haematococcus pluvialis is placed in an attached cultivation system for cultivation, in the cultivation process of the haematococcus pluvialis, an artificial light source is utilized, other growth factors are manually controlled, so that the haematococcus pluvialis grows under the condition of being isolated from the outside world, and the astaxanthin accumulates. According to the method, artificial illumination is utilized for replacing sunlight, underthe condition of being completely isolated from the outside world, the haematococcus pluvialis is cultivated by the attached cultivation system to produce the astaxanthin, the influence of external factors such as weather on the growth of the haematococcus pluvialis and the growth of the astaxanthin is completely avoided, the yearly uninterrupted and stable production is achieved, and the methodis not limited to geographical locations with the high annual sunshine amount and mild climates any more and can be carried out anywhere. The method has the advantages that the output rate per unit floor area is high, and the production efficiency is far higher than that of an existing haematococcus pluvialis cultivation system.

The invention provides a method for preparing water-soluble astaxanthin. The method comprises the following steps: mixing astaxanthin-containing raw materials with an organic acid-containing solutionand then carrying out cell disruption;leaching, wherein natural astaxanthin can interact with naturally existing components such as protein, nucleic acid or polysaccharide in the raw materials, so that the water-soluble astaxanthin is directly prepared, astaxanthin does not need to be modified, and the astaxanthin is natural in source, simple in operation process, low in equipment requirement, lowin production cost, environmentally friendly, safe, free of organic solvent residues and easy to industrialize.

The invention relates to a method for preparing microbial cultures through solid state fermentation of shrimp shells, and relates to the field of feed processing. The method comprises the following steps of taking shrimp shells, soybean meal, bran and composite trace elements in a certain mass ratio, performing mixing to obtain a mixture as a fermentation substrate, regulating the moisture content, adding 3 kinds of bacteria of phaffia rhodozyma, bacillus subtilis, plant lactobacillus and the like, controlling fermentation condition, and adopting combination of aerobic fermentation and anaerobic fermentation so as to obtain the products namely the microbial cultures. When the method disclosed by the invention is used for producing the microbial cultures through solid state fermentation ofshrimp shells, the possibility that chitin in the shrimp shells is converted into functional chitosan is effectively reduced, the protein content is increased, and the method is suitable for industrial production of feeds.

A method for culturing red-hair yeast with shrimpanin synthesis accelerator. Use shrimpanin synthesis former-rich fruits, vegetables and tea juice such as fresh orange, spinach, tea leaf, etc as materias, and prepare shrimpanin synthesis accelerator by pressing juice or immersion. Add the shrimpanin synthesis accelerator in red-hair yeast medium at different fermention time; ferment for some time; collect yeast cell. Also we can furtherly do cell wall breaking treatment and extrat shrimpanin from the cell with acetone. The red-hair yeast achieved has high shrimpanin content, and the shrimpanin content can be advanced largely just by adding some shrimpanin synthesis accelerator. It has the advantages of investment little, raw materials cost little, and so on. Both the achieved red-hair yeast and the shrimpanin extracted from it can be used as feedingstuff additive for top-grage aquatic products and birds, and can make the culturing products have high commondity value.

The invention relates to a method for quickly screening high-yield astaxanthin phaffia rhodozyma strains (Phaffia rhodozyma CGMCC2.1557). The method includes steps: 1) activating and culturing wild-type phaffia rhodozyma strains in a constant-temperature incubator at 22-25 DEG C, taking a small quantity of single colonies to add into a seed medium, and performing shake cultivation by 120-130r / min;2) performing ARTP (atmospheric and room temperature plasma) mutagenesis of the wild-type phaffia rhodozyma strains for 150-180s; 3) transferring bacteria liquid into a centrifugal tube after mutagenesis, and performing gradient dilution and flat plate coating; 4) transferring the strains to a 24-pore plate, performing shake cultivation for 45-50h, and observing through a confocal laser scanningmicroscope to screen candidate mutant strains; 5) carrying out shake cultivation in the seed medium; 6) performing proportional inoculation of seed liquid in a fermentation medium, and starting shakecultivation; 7) ultrasonically extracting astaxanthin for 1-1.2h, and detecting the astaxanthin content of fermented liquid by high performance liquid chromatography; 8) screening out high-yield astaxanthin mutant strains, wherein yield is increased by 25-55% compared with that of the mild-type strains. The high-yield astaxanthin phaffia rhodozyma strains are screened by two steps including colonycolor primary screening and cell fluorescence intensity re-screening, the method is simple in operation, safe and free of pollution, the mutant strains are stable, and industrial production cost is reduced.

The present invention provides haematococcus pluvialis JNU 35 with high astaxanthin yield, and a culture method and an application thereof. The strain is named as haematococcus pluvialis JNU 35 and preserved in China General Microbiological Culture Collection Center, Beijing, China on 7th, September, 2018, and has a preservation number of CGMCC No.16438. At the same time, the culture method of thehaematococcus pluvialis JNU 35 is provided and biomass can be rapidly accumulated. The present invention also provides an astaxanthin induction method of the haematococcus pluvialis JNU 35. The method effectively balances synchronous and rapid accumulation of biomass and astaxanthin content through innovative optimization of a two-step strategy, greatly improves the haematococcus pluvialis biomass and astaxanthin content, can obviously reduce cost for generating astaxanthin, has important application value in actual production, and has good industrial production prospect.

The present invention relates to a method for reducing the relative ratio of 3-hydroxy-3′,4′-didehydro- β, Ψ-caroten-4-one (HDCO) to astaxanthin in a composition containing astaxanthin and HDCO by contacting the composition with an acidic medium having a pH of 3 or less and / or a basic medium having a pH of 9 or greater, and also relates to a method for producing an astaxanthin-containing composition which includes reducing the relative ratio of HDCO by the above method. By means of the method of the present invention, the relative ratio of HDCO, the biological function of which is not known, in an astaxanthin-containing composition can be easily reduced.

The invention discloses a screening method of a haematococcus pluvialis mutant with high yield of astaxanthin, relates to the field of astaxanthin culture and screening, and aims to solve the problems of time consumption and labor consumption in mutant screening in the prior art. The technical scheme is as follows: a mutant algal strain is obtained by performing ultraviolet mutagenesis and screening on haematococcus pluvialis 6W3, the screening method is easy to operate and is low in time consumption in the process, high-throughput screening can be achieved, and the working efficiency of screening is greatly improved. The obtained mutant grows quickly, has high astaxanthin content and has a good astaxanthin development prospect; when the haematococcus pluvialis is cultured, the single algae colony with large volume and dark green color is selected to be cultured, the content of astaxanthin in the single algae colony with large volume and dark green color is rich, the concentration of astaxanthin in the extract liquor is high, and the yield in the subsequent extraction and purification process can be effectively improved.

The invention discloses a preparation method of dried shrimps. Shrimp is soaked in tea polyphenol solution at low temperature, boiled in water, soaked in antistaling agent, soaked in osmotic dehydration liquid and ultrasonically dehydrated, vacuum freeze-dried and packaged to obtain dried shrimp products. The preparation method of the present invention can maintain the color and luster of shelled shrimps and inhibit the oil oxidation process, thereby improving the storage stability of dried shrimp products and inhibiting browning; the yellowness value and volatile base nitrogen value of the obtained dried shrimps are obviously reduced, and the storage Astaxanthin has a good protective effect during the process, and astaxanthin in shrimp is a natural antioxidant, which plays an important role in inhibiting oil oxidation and protecting color. Therefore, the dried shrimp prepared by the present invention is obviously better than the dried shrimp currently on the market in terms of color protection, oxidation inhibition and shelf life extension, and the operation steps are practical.

The invention discloses application of morpholine in controlling unsaponifiable matter content and increasing DHA grease content in a schizochytrium limacinum fermentation process, and aims to reduce sterol content and increase grease content in the schizochytrium limacinum fermentation process by exogenously adding morpholine in the schizochytrium limacinum fermentation process. In addition, optimum addition concentration of morpholine is determined according to the biomass of cell growth of schizochytrium limacinum, the oil yield and the unsaponifiable matter content index; compared with the situation that 1.8 g / L of morpholine is added, the sterol content is reduced by 64.86%, the oil yield is increased by 67.51%, the DHA content is increased by 25.5%, and the astaxanthin content is increased by 85%. By means of the technical scheme, the content of DHA grease and astaxanthin produced by the schizochytrium limacinum is obviously improved. In the invention, the morpholine is applied to regulation and control of the unsaponifiable matter content of schizochytrium limacinum for the first time, wherein a new thought is provided for increasing the content of grease in schizochytrium limacinum, and the method has huge potential in the aspect of industrial production.

The invention provides Haematococcus pluvialis JNU35 with high astaxanthin production and its cultivation method and application. The algae strain is named Haematococcus pluvialis JNU35, and it was deposited in the General Microbiology Center of China Committee for the Collection of Microorganisms in Beijing, China on September 7, 2018, with the preservation number CGMCC No.16438. At the same time, it provides a cultivation method of the Haematococcus pluvialis JNU35, which can quickly accumulate biomass. The present invention also provides a method for inducing astaxanthin in Haematococcus pluvialis JNU35, through the innovation and optimization of the two-step method strategy, it effectively balances the synchronous and rapid accumulation of biomass and astaxanthin content, greatly improving The biomass of Haematococcus pluvialis and the content of astaxanthin are increased, the cost of astaxanthin production can be significantly reduced, and it has important application value in actual production and has a good prospect for industrial production.

The invention discloses a synchronous enrichment method of lipide and protein in pandalus borealis processing by-products. The synchronous enrichment method comprises the following steps of (1) pretreating raw materials; (2) performing extraction: adding an oil phase to an enzymolysis system obtained in the step (1), then inputting air currents to the system through a microbubble generator, and then collecting crude extraction solutions in a manner of filtering or centrifuging manner; (3) constructing a multiphase separation system; (4) treating a lipide phase: collecting an upper phase solution in a layering system obtained in the step (3), then performing decompressed concentration, and recovering a solvent so as to obtain a lipide extract; (5) treating protein: collecting interlayer substances obtained in the step (3), and performing drying so as to obtain a protein extract; and (7) performing an aqueous phase: collecting a lower-layer salt-containing aqueous phase solution obtainedin the step (3), and performing recycling. A new thinking is provided for further developing and increasing the added value of the pandalus borealis, and a new value growth point is provided for theindustry chain.

The invention discloses a method of utilizing seawater to facilitate haematococcus pluvialis to produce natural astaxanthin, and relates to the technical field of artificial cultivation of haematococcus pluvialis. The method comprises the following steps: utilizing seawater to prepare an improved culture medium of haematococcus pluvialis, adding seawater step by step to induce vegetative growth of haematococcus, cell type transformation and accumulation of astaxanthin. The method can be applied to cultivate haematococcus pluvialis at large scale at the coastal region rich in the seawater resource to produce the natural astaxanthin; compared with the conventional cultivation method of using freshwater, the method has the advantages of short production cycle, high astaxanthin content, and low material cost, and can achieve a wide industrialized application prospect.

The invention provides a method for preparing water-soluble astaxanthin. The method mixes a raw material containing astaxanthin with a solution containing an organic acid, then breaks the cells, and then leaches, so that natural astaxanthin and raw materials can be achieved. The interaction between naturally occurring proteins, nucleic acids or polysaccharides, etc., so as to directly produce water-soluble astaxanthin, without further modification of astaxanthin, and the source of astaxanthin is natural, the operation process is simple, the equipment requirements are low, and the production Low cost, green and safe, no organic solvent residue, easy to realize industrialization.

The invention discloses a method for promoting high yield of astaxanthin from phaffia rhodozyma by TiO2 / Al2O3. The method is characterized by comprising the following steps: a sterile fermentation culture medium added with a nano TiO2 / Al2O3 mother solution is inoculated with a phaffia rhodozyma seed solution for fermentation culture, the nano TiO2 / Al2O3 mother solution is a nano titanium dioxide mixed solution taking a nano aluminum oxide aqueous dispersion as a carrier, and an unbalanced pH value inside and outside phaffia rhodozyma cells is kept during fermentation, so that the stress reaction of the phaffia rhodozyma is stimulated, and a metabolic pathway in the phaffia rhodozyma proceeds towards an astaxanthin production direction. The method has the advantages that the nanometer aluminum oxide aqueous dispersion is used as a carrier, the nanometer titanium dioxide is treated and dissolved into the fermentation culture medium, the unbalanced pH value inside and outside the phaffia rhodozyma cells is kept during fermentation, the stress reaction of the phaffia rhodozyma is stimulated, the metabolic pathway in the phaffia rhodozyma proceeds towards the astaxanthin production direction, and thus, the content of the astaxanthin is increased.