49results about How to "Increased astaxanthin content" patented technology

The invention discloses a method for increasing the content of astaxanthin in haematococcus pluvialis. The method comprises the step of adding monoethanolamine (MEA) into a culture solution at an immobile stage of culture of haematococcus pluvialis until the final concentration is 50-400 mg/L. The method for increasing the content of astaxanthin in haematococcus pluvialis is a new technical routefor increasing the content of astaxanthin in haematococcus pluvialis, the content of astaxanthin in cells is significantly increased, the culture time is shortened, and the culture efficiency is improved.

The invention discloses a method of utilizing seawater to facilitate haematococcus pluvialis to produce natural astaxanthin, and relates to the technical field of artificial cultivation of haematococcus pluvialis. The method comprises the following steps: utilizing seawater to prepare an improved culture medium of haematococcus pluvialis, adding seawater step by step to induce vegetative growth of haematococcus, cell type transformation and accumulation of astaxanthin. The method can be applied to cultivate haematococcus pluvialis at large scale at the coastal region rich in the seawater resource to produce the natural astaxanthin; compared with the conventional cultivation method of using freshwater, the method has the advantages of short production cycle, high astaxanthin content, and low material cost, and can achieve a wide industrialized application prospect.

The invention discloses a natural pigment feed additive for freshwater aquaculture of vannamei. The natural pigment feed additive for the freshwater aquaculture of the vannamei comprises the following components in parts by weight: 5-9 parts of dunaliella salina powder, 5-7 parts of carrot powder, 1-8 parts of spirulina powder and 22-38 parts of pigment stabilizer. 0.1-2% of the natural pigment feed additive for the freshwater aquaculture of the vannamei by mass is added into feed for the freshwater aquaculture of the vannamei, so that the astaxanthin content in the body of the adult vannamei can be obviously improved to improve the color, and the non-specific immunity of the vannamei can be improved to reduce diseases and further the economic benefit is improved.

The cultivating and breeding method of marigold features the direct field seeding and transplanting. The new variety of adonis amurensis obtained with wild adonis amurensis and through domestication, scientific management and optimizing breeding. The obtained adonis amurensis has synchronous blooming, strong pest and disease resistance, high astaxanthin content and other advantages, and is suitable for commercial planting in large area.

The invention discloses a biological reaction kettle and a method for promoting proliferation and reddening of haematococcus pluvialis. The biological reaction kettle comprises a main body reaction kettle, wherein the main body reaction kettle is provided with a pH (potential of hydrogen) sensing head interface; the pH sensing head interface is connected with a pH controller; one end of the pH controller is connected with an industrial carbon dioxide bottle, and the other end of the pH controller is connected with the main body reaction kettle through an air intake adjusting valve; a filter is arranged at an outlet of the industrial carbon dioxide bottle; an LED (light emitting diode) lamp tube is arranged in the main body reaction kettle, and is connected with an LED driving controller; gas explosion stone is arranged at the bottom wall of the inner side of the main body reaction kettle, and is connected with a T-level filter through the air intake adjusting valve; the T-level filter is connected with an industrial air compressor; the LED lamp tube is connected with one end of a water cooling machine through the air intake adjusting valve, and the other end of the water cooling machine is connected with the LED lamp tube. The biological reaction kettle has the advantages that the problem of adverse influence by weather, light ray, pollution, environment temperature and the like in the existing traditional microalgae culture type is solved, and the growth speed of microalgae is greatly improved.

The present invention discloses a production process for extracting natural astaxanthin by utilizing supercritical fluid CO2. It is characterized by that it utilizes natural plant adonis chrysocyathus, places it in a supercritical fluid extraction equipment and adopts the processes of heating, boosting and injecting CO2 fluid so as to obtain natural astaxanthin.

The present invention relates to a novel method for producing astaxanthin by using microalgae. The method comprises: heterotrophic cultivation of microalgae, dilution, photo-induction, collection of microalgal cells, and extraction of astaxanthin. The method according to the present invention takes full advantages of rapid growth rate in the heterotrophic stage and fast accumulation of astaxanthin in the photo-induction stage by using a large amount of microalgal cells obtained in the heterotrophic cultivation stage, so as to greatly improve the astaxanthin production rate and thereby achieve low cost, high efficiency, large scale production of astaxanthin by using microalgae. The method not only provides an important technical means to address the large scale industrial production of astaxanthin through microalgae but also ensures an ample source of raw material for the widespread utilization of astaxanthin.

The invention discloses a fermentation culture medium and method for producing astaxanthin through fermentation of Phaffia rhodozyma. Each 1L of water in the fermentation culture medium contains the following components by weight: 80g-140g of glucose, 5g-8g of yeast extract powder, 5g-8g of malt extract powder, 6g-10g of proteins, 5g-9g of ammonium sulfate, 0.5g-2.5g of magnesium sulfate, 10g-30gof tomato powder and 0.2%-0.5% (volume fraction) of absolute ethyl alcohol. The invention discloses the culture medium and method for highly producing astaxanthin by virtue of Phaffia rhodozyma. By carrying out fermentation through the method, the biomass of thalli and the content of astaxanthin are high, the oxidation resistance of astaxanthin is strong and is equal to 550 times of that of vitamin E and 10 times of that of beta-carotene. Astaxanthin has physiological effects of enhancing the immunity, inhibiting tumors, eliminating in-vivo free radicals and the like and has wide application prospects in health products, medicines, cosmetics and the like.

The invention discloses a selenium-rich chlorella based functional fish feed and a preparation method of the selenium-rich chlorella based functional fish feed, the selenium-rich chlorella based functional fish feed comprises a rapeseed dreg, a rice bran, a flour, a fish meal, a soybean meal, a compound mineral substance, multi-vitamins, monocalcium phosphate, choline chloride, and a selenium-richchlorella powder in the formula, and the selenium-rich chlorella powder is prepared by adding 2 mg/L sodium selenite into a BG-11 culture medium under the optimized condition, culturing, finally homogenizing and breaking a wall after enzymolysis. The high selenium accumulating capability and the organic selenium transforming capability of the chlorella are used, the selenium content of the feed and the proportion of the organic selenium are effectively increased, the safety and the bioavailability of the selenium are strengthened, the economic benefit is further increased while the physique of fish and the meat quality are improved, the increase of the selenium content of the culture medium also promotes the synthesis of astaxanthin of the chlorella, and the immunity of a fish body is improved. The organic selenium-rich fish provides the technical support for the industrial development of selenium-rich fish foods on strengthening the immunity of the human body and resisting aging andcancers.

The invention relates to a ship-borne Antarctic krill powdering process. The process comprises the following steps: (1) material preparation: feeding Antarctic krill into a feeding pump, (2) scraper heater heating and digester heating, (3) material separation, (4) material drying, (5) material cooling, (6) material crushing, (7) liquid treatment, and (8) wastewater treatment. By adopting the production process of the invention, the yield of the product is obviously improved, the astaxanthin content and the protein content of the product are also obviously improved, and the quality of the product is significantly higher than that of shrimp powder produced by traditional methods. After the wastewater is evaporated, all resources in the entire process are fully utilized without any waste of resources.

The invention discloses a method for producing astaxanthin by utilizing phaffia rhodozyma. The method comprises the following steps: 1) grinding and crushing shrimp offal to obtain milled slurry, boiling and cooling the milled slurry, adding compound protease and papain to perform enzymolysis so as to obtain enzymatic hydrolysate; adding straw cellulose dry matters, ammonium sulfate and molasses into the enzymatic hydrolysate, uniformly mixing, and autoclaving to obtain a fermentation matrix; 2) activating phaffia rhodozyma; 3) inoculating the activated phaffia rhodozyma into an expanded medium, culturing under stirred conditions for 45-50 hours, mixing the fermentation matrix, introducing sterile oxygen for continuously culturing for 45-50 hours, introducing mixed gas composed of sterilecarbon dioxide, oxygen and ethylene, wherein the volume ratio of the carbon dioxide to oxygen to ethylene is 2:1:0.03, and the ventilatory capacity is 0.2V/(V.min), and continuously culturing for 45-50 hours so as to obtain the phaffia rhodozyma; and 4) extracting and purifying, thereby obtaining the astaxanthin. The method disclosed by the invention has the characteristics of low production cost,high benefits and the like.

The invention relates to a method for static and dynamic synergistic extraction of astaxanthin by wall-broken lactobacillus supercritical CO2. The method comprises the following steps: adding the dried plant lactobacillus to ethanol, and after ball-milling wall-breaking, obtaining the lactobacillus bacteria slurry as an extraction raw material; mixing diatomite and lactobacillus bacteria slurry, and adding a mixture to an extraction vessel; setting the supercritical extraction temperature to 35-60 DEG C and the supercritical extraction pressure to 10-40 MPa, and flowing supercritical CO2 fromabove the extraction vessel, when the supercritical extraction temperature and the supercritical extraction pressure reach a set value and are stabilized, closing a valve (V2), and cutting off the extraction kettle and a separation vessel; and after the high-pressure solubilization static extraction is completed, regulating the valve and introducing the supercritical CO2 for dynamic synergistic extraction. The method is used for extracting astaxanthin from lactobacillus, has the advantages of low cost, simple operation steps, and high extraction rate, no other organic solvent consumption except ethanol is required, and the whole process is green, low energy consumption and suitable for commercial application.

The invention provides a method for preparing water-soluble astaxanthin. The method comprises the following steps: mixing astaxanthin-containing raw materials with an organic acid-containing solutionand then carrying out cell disruption;leaching, wherein natural astaxanthin can interact with naturally existing components such as protein, nucleic acid or polysaccharide in the raw materials, so that the water-soluble astaxanthin is directly prepared, astaxanthin does not need to be modified, and the astaxanthin is natural in source, simple in operation process, low in equipment requirement, lowin production cost, environmentally friendly, safe, free of organic solvent residues and easy to industrialize.

The invention relates to a method for preparing microbial cultures through solid state fermentation of shrimp shells, and relates to the field of feed processing. The method comprises the following steps of taking shrimp shells, soybean meal, bran and composite trace elements in a certain mass ratio, performing mixing to obtain a mixture as a fermentation substrate, regulating the moisture content, adding 3 kinds of bacteria of phaffia rhodozyma, bacillus subtilis, plant lactobacillus and the like, controlling fermentation condition, and adopting combination of aerobic fermentation and anaerobic fermentation so as to obtain the products namely the microbial cultures. When the method disclosed by the invention is used for producing the microbial cultures through solid state fermentation ofshrimp shells, the possibility that chitin in the shrimp shells is converted into functional chitosan is effectively reduced, the protein content is increased, and the method is suitable for industrial production of feeds.

A method for culturing red-hair yeast with shrimpanin synthesis accelerator. Use shrimpanin synthesis former-rich fruits, vegetables and tea juice such as fresh orange, spinach, tea leaf, etc as materias, and prepare shrimpanin synthesis accelerator by pressing juice or immersion. Add the shrimpanin synthesis accelerator in red-hair yeast medium at different fermention time; ferment for some time; collect yeast cell. Also we can furtherly do cell wall breaking treatment and extrat shrimpanin from the cell with acetone. The red-hair yeast achieved has high shrimpanin content, and the shrimpanin content can be advanced largely just by adding some shrimpanin synthesis accelerator. It has the advantages of investment little, raw materials cost little, and so on. Both the achieved red-hair yeast and the shrimpanin extracted from it can be used as feedingstuff additive for top-grage aquatic products and birds, and can make the culturing products have high commondity value.

The invention relates to a method for quickly screening high-yield astaxanthin phaffia rhodozyma strains (Phaffia rhodozyma CGMCC2.1557). The method includes steps: 1) activating and culturing wild-type phaffia rhodozyma strains in a constant-temperature incubator at 22-25 DEG C, taking a small quantity of single colonies to add into a seed medium, and performing shake cultivation by 120-130r/min;2) performing ARTP (atmospheric and room temperature plasma) mutagenesis of the wild-type phaffia rhodozyma strains for 150-180s; 3) transferring bacteria liquid into a centrifugal tube after mutagenesis, and performing gradient dilution and flat plate coating; 4) transferring the strains to a 24-pore plate, performing shake cultivation for 45-50h, and observing through a confocal laser scanningmicroscope to screen candidate mutant strains; 5) carrying out shake cultivation in the seed medium; 6) performing proportional inoculation of seed liquid in a fermentation medium, and starting shakecultivation; 7) ultrasonically extracting astaxanthin for 1-1.2h, and detecting the astaxanthin content of fermented liquid by high performance liquid chromatography; 8) screening out high-yield astaxanthin mutant strains, wherein yield is increased by 25-55% compared with that of the mild-type strains. The high-yield astaxanthin phaffia rhodozyma strains are screened by two steps including colonycolor primary screening and cell fluorescence intensity re-screening, the method is simple in operation, safe and free of pollution, the mutant strains are stable, and industrial production cost is reduced.

The present invention relates to a method for reducing the relative ratio of 3-hydroxy-3′,4′-didehydro- β, Ψ-caroten-4-one (HDCO) to astaxanthin in a composition containing astaxanthin and HDCO by contacting the composition with an acidic medium having a pH of 3 or less and/or a basic medium having a pH of 9 or greater, and also relates to a method for producing an astaxanthin-containing composition which includes reducing the relative ratio of HDCO by the above method. By means of the method of the present invention, the relative ratio of HDCO, the biological function of which is not known, in an astaxanthin-containing composition can be easily reduced.

The invention discloses a method for promoting high yield of astaxanthin from phaffia rhodozyma by TiO2/Al2O3. The method is characterized by comprising the following steps: a sterile fermentation culture medium added with a nano TiO2/Al2O3 mother solution is inoculated with a phaffia rhodozyma seed solution for fermentation culture, the nano TiO2/Al2O3 mother solution is a nano titanium dioxide mixed solution taking a nano aluminum oxide aqueous dispersion as a carrier, and an unbalanced pH value inside and outside phaffia rhodozyma cells is kept during fermentation, so that the stress reaction of the phaffia rhodozyma is stimulated, and a metabolic pathway in the phaffia rhodozyma proceeds towards an astaxanthin production direction. The method has the advantages that the nanometer aluminum oxide aqueous dispersion is used as a carrier, the nanometer titanium dioxide is treated and dissolved into the fermentation culture medium, the unbalanced pH value inside and outside the phaffia rhodozyma cells is kept during fermentation, the stress reaction of the phaffia rhodozyma is stimulated, the metabolic pathway in the phaffia rhodozyma proceeds towards the astaxanthin production direction, and thus, the content of the astaxanthin is increased.