39 results about "Astaxanthin synthesis" patented technology

Protein engineered nucleic acid fragments encoding a CrtO ketolase and a CrtZ hydroxylase are provided with increased astaxanthin synthesis activity. Methods using the present nucleic acid fragments are also provided for increasing or altering astaxanthin production in suitable production hosts.

The invention discloses astaxanthin synthetase of sphingomonas, an encoding gene of astaxanthin synthetase and a method for genetic manipulation of sphingomonas. The invention verifies that the sphingomonas can be used for producing astaxanthin, a biological synthesis way capable of producing the astaxanthin is contained, an MEP way and a carotenoid synthesis way are used for producing the astaxanthin in the sphingomonas, and the fact that in the sphingomonas, crtE and crtZ having relatively low homology with the known genes have the function of synthesizing the astaxanthin, can be further proven. Both the enzyme and the encoding gene thereof required by the astaxanthin biosynthetic way of the sphingomonas are not reported in the prior art, so that more resources are provided to the biosynthetic metabolism and transformation of the carotenoid. Genetic manipulation can be carried out on the sphingomonas by taking pBBR1MCS2 as a carrier and taking EGFP as a reporter gene, so that a foundation is laid for the genetic modification of the environmental microorganism of the type and the finding of the degradation mechanisms.

The invention provides a method for inducing algae cells to efficiently synthesize astaxanthin, which belongs to the technical field of astaxanthin synthesis, comprising: a) settling and concentrating high-yield astaxanthin algae liquid in the logarithmic growth phase; b) adding to the concentrated algae liquid After freezing the solution, remove the supernatant, pre-freeze and quickly freeze and store it; c) Induce the cultivation of the algae liquid under 420-440nm blue light irradiation; d) Add fermentation aids to the carbon-free combined medium containing glucose for shaking fermentation nourish. The beneficial effect is: the method helps to reduce the damage to the algae cells during the frozen storage of the algae liquid. Firstly, the blue light of a specific wavelength is irradiated during the culture process to induce the change of the cell shape and promote the synthesis of astaxanthin, and then During the fermentation process, additives were added to increase the flux of pyruvate and acetyl CoA, strengthen the PP pathway and TCA cycle, and improve the metabolic synthesis of astaxanthin.

The invention provides a DNA (deoxyribonucleic acid) sequence represented by SEQ ID NO.5 for coding beta-carotene hydroxylase, a recombinant expression vector and a host cell, wherein the recombinant expression vector is constructed by the DNA sequence, a DNA sequence represented by SEQ ID NO.6 for coding beta-carotene ketoIase and a plasmid, and the host cell is transformed by the abovementioned recombinant expression vector. The invention also provides a method for synthesizing astaxanthin by apple trees to improve photooxidation resistance, which comprises the following steps: a) constructing the recombinant expression vector pCAMBIA1301-bkt-crtR-B; b) transforming competent agrobacterium tumefaciens CHA105 by the recombinant expression vector pCAMBIA1301-bkt-crtR-B; and c) transforming the apple tissue culture seedlings to obtain the transgenic plants via the agrobacterium tumefaciens. According to the invention, the key enzymic genes bkt and crtR-B synthesized by astaxanthin are transferred into an apple genome by the genetic transformation, the apples rich in astaxanthin are cultivated by regulating and controlling the metabolic pathways, thereby improving photosynthetic efficiency and preventing sunburn, and dramatically overcoming the shortcomings in the prior art.

CrtW carotenoid ketolases are provided that are useful for the production of astaxanthin and other cyclic ketocarotenoids. The mutant ketolase genes of the present invention encode polypeptides characterized by an improvement in astaxanthin synthesis activity when converting cyclic hydroxylated carotenoid intermediates into astaxanthin. Expression of the mutant carotenoid ketolases in heterologous hosts enabled increased production of astaxanthin relative to the Sphingomonas melonis DC18 CrtW.

The invention discloses a phaffia rhodozyma strain obtained by efficiently an over-expressing endogenous astaxanthin synthetase gene. The invention provides a phaffia rhodozyma genetic engineering strain capable of producing astaxanthin with high yield, obtained by over-expressing an endogenous astaxanthin synthetase gene. A phaffia rhodozyma genetic engineering strain (MK19-pGBKT7crtS) capable of producing astaxanthin with high yield is obtained by over-expressing the astaxanthin synthetase gene crtS by virtue of free plasmids and the strain is named CSR19. An endogenous ribosome bind site sequence (Rbs. sequence for short) exists in front of the crtS gene expressed by the strain. By virtue of fermentation cultivation and in comparison with a receptor strain MK19, the astaxanthin yield of the engineering strain is increased by 33.5%, and the engineering strain is good in stability, and thus, the phaffia rhodozyma strain has a good application prospect in the feed industry.

Synthesis of astacin is carried out by dispersing astaxanthin with mass concentration 1-6g / L and alkali in proportion 1:2-20 in alcohol solvent, agitating while inducing into oxygen, reacting at 30-80 degrees C to obtain reactant, removing alcohol solvent to obtain solid crude product, dissolving the crude product by methylene chloride, filtering, dissolving the filter residue by water to obtain alkali reactive liquid, neutralizing by acid to pH value 4-6, filtering, washing by water and drying to obtain final product. It is simple and convenient, has more yield and no by-product.

The invention provides a method for efficient astaxanthin synthesis by induction of chromochloris zofingiensis. The method includes steps: S1, activation culture, namely performing inoculation of chromochloris zofingiensis cells into a culture medium to obtain seed liquid by activation culture, to be more specific, subjecting the chromochloris zofingiensis cells to activation culture to a logarithmic phase, and taking the culture liquid in the logarithmic phase as the seed liquid; S2, induction culture, to be more specific, performing inoculation of the seed liquid obtained at the step S1 into an induction culture medium to carry out photoinduction culture, wherein a light source is white light or blue light, intensity of the light source is 60-330Mumol.m<-2>.s<-1>, a light-dark cycle is 12:12(h)-24:0(h), and the induction culture medium comprises glucose with concentration being 9-30g / L and sodium nitrate with concentration being 0-0.75g / L, and pH value of the induction culture medium is 6.0-7.0. According to the method, efficient astaxanthin synthesis can be realized by induction of chromochloris zofingiensis.

The present invention relates to a new method for the synthesis of astaxanthin, which consists of the following reactions: ∴ The method uses the by-product trans-C in the production of vitamin A 6 Alcohol is used as the starting material, and astaxanthin is synthesized through four-step reactions, which is a process with good reaction selectivity, high reaction yield, less pollution and simple operation.

The present invention relates to the process of regualting haemtococcus pluvialis population density and synthesizing and accumulating astaxanthin as intracellular secondary catabolite in a photobiological reactor system. The process includes establishing a photobiological reactor system with main reactor made of transparent material, outside controllable lighting facility, speed regulating vane wheel stirrer, water rolling wheel, ventilating and oxygen supplying unit, nutritious liquid compounding and temperature controlling unit and sterilizing unit; antering environment conditions to regulate haemtococcus pluvialis population density and synthesize, accumulate and collect astaxanthin; low temperature drying; etc. The natural astaxanthin is a kind of carotinoid with important physiological functions and very strong antioxidant activity.

A method for culturing red-hair yeast with shrimpanin synthesis accelerator. Use shrimpanin synthesis former-rich fruits, vegetables and tea juice such as fresh orange, spinach, tea leaf, etc as materias, and prepare shrimpanin synthesis accelerator by pressing juice or immersion. Add the shrimpanin synthesis accelerator in red-hair yeast medium at different fermention time; ferment for some time; collect yeast cell. Also we can furtherly do cell wall breaking treatment and extrat shrimpanin from the cell with acetone. The red-hair yeast achieved has high shrimpanin content, and the shrimpanin content can be advanced largely just by adding some shrimpanin synthesis accelerator. It has the advantages of investment little, raw materials cost little, and so on. Both the achieved red-hair yeast and the shrimpanin extracted from it can be used as feedingstuff additive for top-grage aquatic products and birds, and can make the culturing products have high commondity value.

The invention discloses a method for synthesizing an astaxanthin intermediate by utilizing 2, 6, 6-trimethyl-3, 4-dihydroxy-2-cyclohexene-1-one. The method comprises the following steps of: dispersing the 2, 6, 6-trimethyl-3, 4-dihydroxy-2-cyclohexene-1-one in a non-polar solvent, adding an acid catalyst, dripping enol ether at room temperature for reaction, adding tertiary amine for termination reaction, then cooling, directly dissolving a reaction solution into an ethanol water solution with a compound obtained by reaction of an alkynyl reagent, then dripping into the ethanol water solution dissolving an acid, adding sodium carbonate for termination reaction after the end of dripping, performing reduced pressure distillation and recovery of the solvent, adding water into residues for stratification, extracting a water phase with dichloromethane, merging and drying organic phases, and then concentrating so as to synthesize the astaxanthin intermediate. The method disclosed by the invention has the advantages of high yield of products, low isomerization of the products and the like.