Method of regulating haematococcus pulvialis population density and synthesing and accumulating astaxanthin in photoreactor
A photobioreactor and Haematococcus technology, applied in biochemical equipment and methods, microorganisms, fermentation, etc., can solve the problems of low population density of Haematococcus, obstacles to large-scale production of Haematococcus, and low yield per unit area
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Embodiment 1
[0021] Establish an optimized open large-scale operation system photobioreactor. The main reactor (water tank) is 500×150×45cm in length, width and depth. The bottom and sides are made of 2cm thick colorless and transparent tempered glass. The power of the balance device with adjustable brightness is 30 100W solar lamps and 20 40W energy-saving spotlights; the speed of the speed-regulating paddle impeller agitator in the reactor is 30r / m; the speed of the water wheel is 60r / m; the nutrient solution and temperature control The capacity of the liquid storage tank of the system is 4000L, and the rotational speed of the agitator is 80r / m. The composition of nutrient solution: 45% of clear water, 55% of sea water, potassium nitrate, sodium acetate and other nutrient salts are adjusted according to the physiological needs of different growth stages. Clean water and sea water can be sterilized by the ozone generator. The optimized culture conditions for the pure culture of algal spe...
Embodiment 2
[0023] Establish an optimized open large-scale operation system photobioreactor. The main reactor (water tank) is 220×110×35cm in length, width and depth. The bottom and sides are made of 1.5cm thick colorless and transparent tempered glass. The power of the side equalization device with adjustable brightness is 12 40W fluorescent lamps; the rotational speed of the speed-regulating paddle impeller agitator in the reactor is 20r / m; the rotational speed of the scroll wheel is 20r / m; The stirring speed is 60r / m. Nutrient solution composition: 30% clear water, 70% seawater, potassium nitrate, sodium acetate and other nutrient salts are adjusted according to the physiological needs of different growth stages. Clean water and sea water can be sterilized by the ozone generator. The optimized culture conditions for the pure culture of algal species and the adjustment of population density are as follows: 0.2 g / L of potassium nitrate in nutrient solution, 0.45 g / L of sodium acetate, 0...
Embodiment 3
[0025] Establish an optimized open large-scale operation system photobioreactor. The main reactor (water tank) is 200×100×35cm in length, width and depth. The bottom and sides are made of 1cm thick colorless and transparent tempered glass. The power of the equalization device with adjustable brightness is 9 40W fluorescent lamps; the rotational speed of the speed-regulating paddle impeller agitator in the reactor is 10r / m; the rotational speed of the scroll wheel is 10r / m; The speed of the machine is 20r / m. Nutrient solution composition: 55% clear water, 45% seawater, potassium nitrate, sodium acetate and other nutrient salts are adjusted according to the physiological needs of different growth stages. Clean water and sea water can be sterilized by an ozone generator. The optimized culture conditions for the pure culture of algal species and population density adjustment stage are: nutrient solution concentration potassium nitrate 0.1g / L, sodium acetate 0.3g / L, urea 0.1g / L, m...
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