Method of regulating haematococcus pulvialis population density and synthesing and accumulating astaxanthin in photoreactor

A photobioreactor and Haematococcus technology, applied in biochemical equipment and methods, microorganisms, fermentation, etc., can solve the problems of low population density of Haematococcus, obstacles to large-scale production of Haematococcus, and low yield per unit area

Inactive Publication Date: 2003-01-22
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the main factor hindering the large-scale production of Haematococcus is the high cost caused by the low population density of Haematococcus during the cultivation process and the low yield per unit area.
Haematococcus cultured in a small-scale bioreactor closed system composed of a pipeline photobioreactor or a small immobilized system or an airlift bioreactor, the defect is that the pipe wall is easily covered by algal cell adhesion, resulting in Decreased light transmittance and difficult to clean. Although different types of paddle wheels have been used to stir and mix to minimize the shading effect between algae cells and improve the utilization of light, it is only suitable for small-scale experiments in the laboratory.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Establish an optimized open large-scale operation system photobioreactor. The main reactor (water tank) is 500×150×45cm in length, width and depth. The bottom and sides are made of 2cm thick colorless and transparent tempered glass. The power of the balance device with adjustable brightness is 30 100W solar lamps and 20 40W energy-saving spotlights; the speed of the speed-regulating paddle impeller agitator in the reactor is 30r / m; the speed of the water wheel is 60r / m; the nutrient solution and temperature control The capacity of the liquid storage tank of the system is 4000L, and the rotational speed of the agitator is 80r / m. The composition of nutrient solution: 45% of clear water, 55% of sea water, potassium nitrate, sodium acetate and other nutrient salts are adjusted according to the physiological needs of different growth stages. Clean water and sea water can be sterilized by the ozone generator. The optimized culture conditions for the pure culture of algal spe...

Embodiment 2

[0023] Establish an optimized open large-scale operation system photobioreactor. The main reactor (water tank) is 220×110×35cm in length, width and depth. The bottom and sides are made of 1.5cm thick colorless and transparent tempered glass. The power of the side equalization device with adjustable brightness is 12 40W fluorescent lamps; the rotational speed of the speed-regulating paddle impeller agitator in the reactor is 20r / m; the rotational speed of the scroll wheel is 20r / m; The stirring speed is 60r / m. Nutrient solution composition: 30% clear water, 70% seawater, potassium nitrate, sodium acetate and other nutrient salts are adjusted according to the physiological needs of different growth stages. Clean water and sea water can be sterilized by the ozone generator. The optimized culture conditions for the pure culture of algal species and the adjustment of population density are as follows: 0.2 g / L of potassium nitrate in nutrient solution, 0.45 g / L of sodium acetate, 0...

Embodiment 3

[0025] Establish an optimized open large-scale operation system photobioreactor. The main reactor (water tank) is 200×100×35cm in length, width and depth. The bottom and sides are made of 1cm thick colorless and transparent tempered glass. The power of the equalization device with adjustable brightness is 9 40W fluorescent lamps; the rotational speed of the speed-regulating paddle impeller agitator in the reactor is 10r / m; the rotational speed of the scroll wheel is 10r / m; The speed of the machine is 20r / m. Nutrient solution composition: 55% clear water, 45% seawater, potassium nitrate, sodium acetate and other nutrient salts are adjusted according to the physiological needs of different growth stages. Clean water and sea water can be sterilized by an ozone generator. The optimized culture conditions for the pure culture of algal species and population density adjustment stage are: nutrient solution concentration potassium nitrate 0.1g / L, sodium acetate 0.3g / L, urea 0.1g / L, m...

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Abstract

The present invention relates to the process of regualting haemtococcus pluvialis population density and synthesizing and accumulating astaxanthin as intracellular secondary catabolite in a photobiological reactor system. The process includes establishing a photobiological reactor system with main reactor made of transparent material, outside controllable lighting facility, speed regulating vane wheel stirrer, water rolling wheel, ventilating and oxygen supplying unit, nutritious liquid compounding and temperature controlling unit and sterilizing unit; antering environment conditions to regulate haemtococcus pluvialis population density and synthesize, accumulate and collect astaxanthin; low temperature drying; etc. The natural astaxanthin is a kind of carotinoid with important physiological functions and very strong antioxidant activity.

Description

technical field [0001] The invention relates to a method for adjusting the population density of Haematococcus and the synthesis and accumulation of intracellular secondary metabolite astaxanthin by an optimized open-type large-scale operation system photobioreactor. Background technique [0002] Haemtococcus (Ag.) is a single-celled micro-green algae, the cells of which are about 19-63 microns. Haematococcus motile cells are oval or ovoid with two equal-length flagella. There is a gap between the cell wall and the protoplast, which is filled with gelatinous substances. The cell wall and the protoplast are connected by cytoplasmic filaments. Vegetative reproduction is cell division to form 2, 4, 8 or more daughter cells. Asexual reproduction forms gelatin when the environment is unfavorable. Groups or thick-walled spores, sexual reproduction is isogamous. It is green under normal growth conditions, and the pigments contained in its cells...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/02C12P23/00
Inventor 刘昕袁建平陈峰王江海黄晓霓
Owner SUN YAT SEN UNIV
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