Method of regulating haematococcus pulvialis population density and synthesing and accumulating astaxanthin in photoreactor

Abstract

The present invention relates to the process of regualting haemtococcus pluvialis population density and synthesizing and accumulating astaxanthin as intracellular secondary catabolite in a photobiological reactor system. The process includes establishing a photobiological reactor system with main reactor made of transparent material, outside controllable lighting facility, speed regulating vane wheel stirrer, water rolling wheel, ventilating and oxygen supplying unit, nutritious liquid compounding and temperature controlling unit and sterilizing unit; antering environment conditions to regulate haemtococcus pluvialis population density and synthesize, accumulate and collect astaxanthin; low temperature drying; etc. The natural astaxanthin is a kind of carotinoid with important physiological functions and very strong antioxidant activity.

Description

technical field

[0001] The invention relates to a method for adjusting the population density of Haematococcus and the synthesis and accumulation of intracellular secondary metabolite astaxanthin by an optimized open-type large-scale operation system photobioreactor. Background technique

[0002] Haemtococcus (Ag.) is a single-celled micro-green algae, the cells of which are about 19-63 microns. Haematococcus motile cells are oval or ovoid with two equal-length flagella. There is a gap between the cell wall and the protoplast, which is filled with gelatinous substances. The cell wall and the protoplast are connected by cytoplasmic filaments. Vegetative reproduction is cell division to form 2, 4, 8 or more daughter cells. Asexual reproduction forms gelatin when the environment is unfavorable. Groups or thick-walled spores, sexual reproduction is isogamous. It is green under normal growth conditions, and the pigments contained in its cells...