111 results about "Vegetative reproduction" patented technology

The invention relates to a propagation method of apple seedlings, which is characterized by including the steps: firstly, selecting rootstocks and scions; secondly, storing the rootstocks and the scions; thirdly, performing pre-grafting treatment; fourthly, performing scion grafting; fifthly, performing cuttage on a field; and sixthly, obtaining seedlings. The propagation method has the advantages that firstly, scion grafting is used instead of single budding, so that growth vigor of seedlings subjected to scion grafting is evidently better than that of the seedlings subjected to single budding; secondly, seedling raising time is shortened, production cost is lowered, and factory large-scale raising of seedlings in batch is achieved; thirdly, inter-stock seedlings are propagated with rootstock seeds instead of vegetative propagation, seedling propagation is all subjected to vegetative propagation, and accordingly, uniform sizes of trees in terms of production and cultivation are guaranteed; and the seedling raising time is shortened, and accordingly the speed of applying common apple seedlings and inter-stock nutrimental root seedlings is increased in China.

The invention discloses a rapid propagation technique of Dendrobium candidum axillary buds. According to the technique, Dendrobium candidum axillary buds are used as explants directly to induce fasciculated buds; influences of combinations of different types of hormones with different concentration and different natural extracts on survival and growth of axillary buds, induction and multiplication of fasciculated buds and seedlings are compared, the quantity and quality of seedlings growing from fasciculated buds are researched and explored, the optimal culture medium formula in each stage is screened, and therefore, a fasciculated-bud-way high-efficiency rapid propagation system is established, so that plants obtained through rapid propagation are consistent with parent plants genetically so as to provide basis for restoration of wild population of Dendrobium candidum, and furthermore, wild resources are protected. Compared with vegetative propagation, the rapid propagation technique provided by the invention greatly improves propagation factor. A high-efficiency rapid propagation system of Dendrobium candidum which is an endangered plant provides a technical probability for the large-scale production and is beneficial to the restoration of wild population.

A water eco-environment protection method based on water plant harvesting. The invention relates to a water eco-environment protection method, which is characterized in that water plants are cultivated in a water body or naturally recovered water plants are utilized; nitrogen and phosphorous nutrients are absorbed by water plant growth; the water plants are harvested in growth period by manual work or waterweed harvesting equipment so as to promote the regrowth of the water plants; harvesting is carried out for multiple times, ends in the growth period and is carried out for the last time after the water plants grow seeds or vegetative propagule so as to extract the nitrogen and phosphorus nutrients from water in the form of water plant residues. The invention aims to maximize the accumulated biomass of the water plants during the whole growth period or optimize the water quality purifying effect and simultaneously remove nutrients in the water body and deposits, alleviate the eutrophication process of the water body and prevent the water plants from decaying and polluting the water body once again. The method provided by the invention is suitable for daily ecological maintenance of shallow lakes, urban landscape water, slow flow rivers, small reservoirs, wetlands and ponds.

A method for planting the plant on the vertical rock wall in quarry includes preparing slope surface, arranging anchor rods on the slope surface, arranging the plant growing lattice, arranging composite nutritive bags and nutritive bowls, spreading net on the slope surface, sowing the seeds of proper bush, tree, grass and vine plant, and spray irrigation.

The invention discloses an erythrophloeum fordii cutting propagation method which includes the procedures of stock plant selection, cutting orchard building, stock plant management, cutting slip picking, cutting and post-cutting management. Excellent robust nursery plants free of pest diseases are selected as stock plants and are densely arranged in a rectangular piece to build a cutting orchard, top cutting is conducted to promote sprouting, twigs or semi wood is selected as cutting scions which are inserted in container cups filled with substrates, and cutting seedlings are cultured to obtain excellent robust erythrophloeum fordii cutting seedlings. The method solves the problem that erythrophloeum fordii is difficult in vegetative propagation, has the advantages of being free of geographic limitation, high in propagation speed, simple, easy to operate, high in survival rate and the like, maintains good hereditary character of the erythrophloeum fordii, has great significance on protection and expansion of propagation and planting of the erythrophloeum fordii and has good economical benefit, social benefit and ecological benefit.

The invention discloses a method for planting high quality rice. The method comprises the steps of seed selection, seeding, rice transplanting, a panicle primordium differentiation stage, a heading period and a mature period. When rice ears fall and are golden, the rice can be reaped. Compared with the existing rice planting technology, the method has significant advantages.When rice planting is performed according to the technical scheme, the reaped rice produces full ears, the rice quality and quantity are improved greatly, the germination rate of rice seeds can be improved through the treatment of the rice seeds, fertilizer for rice is applied in the panicle primordium differentiation stage, and the fertilizer for the rice are added to fertilizer through conjugation reaction of bentonite, sodium carbonate and ferrous sulfate, so that the fertilizer efficiency is improved, the soil is improved, the absorption of the rice for the fertilizer is promoted, the rice can enter into the vegetative growth stage and reproductive growth stage side by side, and the vegetative reproduction of the rice is guaranteed to lay a solid foundation for head sprouting. According to the five-year continuous planting experiment statistics, the yield of the rice planting through the method is 18% to 20% higher than the yield of the rice planted by general planting methods.

The invention discloses a softwood cuttage propagation method for Acrocarpus fraxinifolius according to the principle of vegetative propagation. Fine clone seedlings can be propagated in quantity by the softwood cuttage propagation technique for Acrocarpus fraxinifolius, and accordingly high yield and high quality of colonies can be realized. The softwood cuttage propagation method for Acrocarpus fraxinifolius is characterized in that rooting agent GGR-6 is provided by Beijing ABT research and development center, the rooting agent GGR-6 is made into 800-1000mg / L solution at constant volume, talcum powder is added into a container in multiple phases during mixing until the solution is pasty, the lower ends of semi-lignified shoots 10-15cm long are dipped into the paste and inserted into a nonwoven nutrition bag having 5cm diameter and nutrition substance composed of pine bark and sawdust in a ratio of 3:1, water-fertilizer management and disease damage control are performed as required after cuttage, and the shoots grow and survive in two months.

The invention discloses a method for regulating and controlling chrysanthemum flowering season. More particularly, the method comprises the following steps: tender stem apexes are taken from field chrysanthemum plants as an explant; tissue culture is carried out, then a single bud material is established, that is, a certain single bud is reproduced in successive generations; the single bud material is treated by methylation inhibitor for 30 days, the treated material undergoes rooting culture and is finally transplanted to the field for cultivation management. The method of the invention adopts 5-azacytidine chemical treatment to change the epigenetic information of chrysanthemum in tissue culture, therefore, the chrysanthemum blooms 7-9 days in advance, and the effect can be passed on by vegetative propagation, and in the next year, the plants growing from the treated material roots can still bloom 7-9 days in advance.

The invention discloses a detoxification, tissue culture and rapid propagation method of common turmeric, which comprises the explant selection and treatment, induction culture, the virus identification of plant regeneration, proliferation culture, transplanting and other steps. In the invention, on the basis of the MS culture medium, the plant growth regulators such as 6-benzyladenine, kinetin, Alpha-banai acid, adenine sulphate are added for the induction culture of common turmeric apical meristem and the proliferation and rooting of multiple shoot two key stages. With the adoption of the packaged technology and method provided in the invention for the tissue culture and rapid propagation of common turmeric, the difficult problems of common turmeric such as virus accumulation, declining quality and yield reduction caused by propagation coefficient and technical stability are met. Therefore, the invention can provide a seedling-raising method with short cycle, high rate of reproduction, low-cost for the factory production of common turmeric.

The present invention relates to an ultra-low temperature storage method of nutrient propagative flower plant lycoris radiate. Said method includes the following steps: bulb induction, pre-culture of shoot tip with root rudiment and bud rudiment, placing the above-mentioned pre-cultured shoot tip in pretreatment solution to make pretreatment at room temperature for 10-30 min, then placing it into vitrified protective agent to make ice bath treatment for 70-90 min, then transplanting said shoot tip into a freeze tube in which the precooled fresh vitrified protective agent is held and placing said freeze tube into liquid nitrogen so as to make storage.

The present invention relates to a reproduction method of potato seed. Said method includes the following steps : firstly, utilizing detoxified seedlings to reproduce raw seed potato seeds, then utilizing matrix cultivation to reproduce raw seed potato seeds, then using 85-90% of raw seed potato seeds to make native reproduction and can directly supply first-grade potato seeds for production, and utilizing residual 10%-15% of raw seed potato seeds to make cold allopathic reproduction of first-grade potato seeds, and then utilizing said first-grade potato seeds to make cold allopatric reproduction to directly supply and produce second-grade potato seeds.

The invention discloses a method for overcoming topophysis in vegetative propagation of gingkoes, which includes the following steps: taking a healthy and full-sprouted branch being without diseases and insect pests and growing at the middle upper part of a crown on the sunny side as a cutting slip; and adopting the cuttage and stumping method to solve the problem of topophysis during the vegetative propagation of gingkoes. Multiple branches generated after stumping can be directly adopted for cuttage or propagation by grafting, and the main branch can grow vertically. According to the method, the cost is low, the seedling forming speed is high, the rate of survival is high; and the problem of quick breeding of good seed sources, pedigree, clone and excellent single plant of gingkoes can be solved.

The lettuce variety Nascent is disclosed. The invention relates to seeds, plants, plant cells, plant tissue and harvested products as well as to hybrid lettuce plants and seeds obtained by repeatedly crossing plants of Nascent with other lettuce plants. The invention also relates to plants and varieties produced by the method of essential derivation from plants of Nascent and to plants of Nascent reproduced by vegetative reproduction methods including but not limited to tissue culture of regenerable cells or tissue from Nascent.

Provided are transformed chrysanthemum plants having blue flower color, their self-fertilized progenies or cross-fertilized progenies thereof, a vegetative propagated plants thereof, and a part, a tissue or a cell of the plant body. Anthocyanin 3′,5′-O-glucosyltransferase gene (CtA3′5′GT) derived from Clitoria ternatea and flavonoid 3′,5′-hydroxylase gene derived from Campanula (CamF3′5′H) are coexpressed in chrysanthemum petals.

The invention discloses an organic no-earth lawn blanket and the quick growth method. The lawn blanket comprises grass and two layers of organic non-woven fabric with different thickness. It mainly makes the combination of accelerating germination before seeding and simple greenhouse earth protecting production form on grass seeds or nutritive breeding body, not only to avoid influence of outside bad climate, but also create more proper growth condition, largely shortening production period (by about 50%), enhancing the whole benefit and reducing production cost by 40%-50%.

The method of cultivating and planting the sheep grass with containers belongs to the agricultural technology field, which relates to cultivate and plant the sheep grass with specific containers. The invention comprises the following steps: cultivate the packed seedling with containers including purchase seedling packs, choose the place, get soil, make onetime simple plastic greenhouse, fill packs with soil and place them, water them sufficiently one time, sift the selected sheep grass seed on the placed well distributed packs, cover them with soil, water them, control the temperature and prevent pets and cure plant diseases and insect pests; move the seedling including expose the seedling, water them sufficiently before moving them, put the seedling into boxes; seedling planted with containers includes soil working, plant and plant time as well as field care. The sheep grass cultivated with this invention has a good ability of nourishment generation, and high rate of surviving, especially in saline-alkali land, which will be soon covered with the plant. That will achieve an optimal environment benefit, economic benefit and society benefit with low cost and wonderful active effect.

The invention discloses a rapid artificial cultivation method for Callicarpa kwangtungensis Chun which is a plant used in traditional Chinese medicine to solve the problem of lack of a rapid artificial cultivation method for Callicarpa kwangtungensis Chun which is a plant used in traditional Chinese medicine. The method is to cut a strong 1 to 2 year old lignified twig of Callicarpa kwangtungensis Chun into a 10 to 15 centimeter cutting, plant the cutting in a sandy soil with organic fertilizer and a pH value of from 4 to 7, spray foliage application during young shoot stages in summer and fall, keep the height of the seedling within 100 centimeters to shorten and strengthen the seedling and transplant the seedling 7 to 8 months after planting. The method is used for rapid artificial cultivation of Callicarpa kwangtungensis Chun which is a plant used in traditional Chinese medicine and has advantages of small character variation, large propagation coefficient, high working efficiency, low seedling raising cost, working efficiency increased by 300 percent compared with vegetative propagation and propagation by grafiting and so on, 98 percent survival rate, 30,000 seedlings for each mu, an income of 8,000 for each mu and good market prospect.

The invention relates to a tissue culture method of Limonium aureum. The tissue culture method includes: obtaining regenerated seedlings through a callus inducing path and particularly includes following steps: (1), disinfecting seeds; (2), culturing sterile seedlings; (3), performing callus inducing; (4), differentiating adventive buds; (5), performing rooting culture. The tissue culture method has the advantages that the problem that Limonium aureum seedling culturing is low in efficiency and less prone to being influenced by environment factors is solved, and vegetative reproduction efficiency of high-quality germplasm resources of Limonium aureum is improved.

The invention relates to a seedling breeding method using asparagus spores. The method comprises the steps of (1) spore collection, (2) indoor cultivation and (3) sea area hanging cultivation and period control. The seedling breeding method using the asparagus spores has the advantages that provenances needed by large-area popularization can be obtained effectively; indoor storage of asparagus during summer is achieved; seedling breeding is high in stability and strong in controllability; cost and labor and material consumption caused by north-to-south transfer and south-to-north transfer are effectively reduced; compared with vegetative propagation, generative propagation of seedlings has the advantages of being capable of breeding a large number of seedlings with different genetic backgrounds, selectively cultivating seedlings adaptable to different sea areas, and easing idioplasm degeneration to a certain degree; improved variety screening during asparagus breeding work is accelerated.

The invention relates to a method for quick restoration of a scirpusplaniculmis wetland, in particular to a method for quick restoration of a scirpusplaniculmis wetland by means of corm transplantation. The method aims to solve the technical problem that in the prior art, the scirpusplaniculmis wetland is restored slowly. The method comprises the steps of digging scirpusplaniculmis corm; conducting packaging and storage, wherein moisture is controlled to be 80-100%, and temperature is controlled to be 4-5 DEG C; conducting plantation; controlling the plant spacing of scirpusplaniculmis to be 10 cm and line spacing to be 15-25 cm. According to the method, by means of the vegetative reproduction characteristic of scirpusplaniculmis, before scirpusplaniculmis turns green at the beginning of May, unbroken corm is directly planted in the wetland to be restored. The method is used for quick restoration of the scirpusplaniculmis wetland.

The invention belongs to the technical field of vegetative propagation, and particularly relates to a lonicera japonica nursery stock rapid propagation method. The lonicera japonica nursery stock rapid propagation method comprises the steps that the breeding and seedling culturing process of lonicera japonica cuttings is carried out on a rapid propagation seed bed, the rapid propagation seed bed uses perlite with good breathability as main substrate, and a water control system capable of achieving automatic spraying is arranged on the seed bed additionally; drugs capable of promoting the lonicera japonica cuttings to root is prepared into mud, the base portions of the lonicera japonica cuttings are dipped in the mud promoting rooting and are dried in the shade until the mud promoting the rooting is coagulated, the rooting drugs are made to act on the base portions of the cuttings for a long time, and therefore the rooting rate and rooting effects are improved. When the cuttings are cultured on the seed bed, the cuttings are made to generate calluses ahead of time or to shorten rooting time by spraying Yuhualing 1 leaf fertilizer. Through the application of the lonicera japonica nursery stock rapid propagation method, the usage of raw materials and propagation time when the rapid propagation of the lonicera japonica is carried out both have no limitation, and the lonicera japonica nursery stock rapid propagation method is quite suitable for factory-like, large-scale and intensive production of the seedling cultivation of lonicera japonica nursery stocks.

The invention discloses a cutting propagation method of Artocarpus nanchuanensis and belongs to the technical field of vegetative propagation. The method includes the steps of selection of cutting substrate, selection of cuttings, treatment of cuttings, selection of cuttage time, post-cuttage management, transplanting time selection and the like. The cuttage success rate already reaches 54%. The success in cutting propagation of Artocarpus nanchuanensis solves the problem that seedling demanded sources from plant market are few and meets the market demand for Artocarpus nanchuanensis seedlings. The number of species populations of Artocarpus nanchuanensis is increased, and ex-situ conservation is benefited. Superior character of fine stock plants is maintained by the vegetative propagation of the fine stock plants.

The invention provides a new form of vegetative propagating material, referred to as “the grass plantlet”, as well as a method for producing it. The new vegetative planting material and the method by which it is obtained make it possible to replicate and increase both warm and cool season grasses more rapidly than by the conventional methods of seeding, sprigging, or plugging. Three to five new, clean, and pure generations of a newly developed seeded or vegetative cultivar can be obtained each year. Outcrossing, the need for burning and possibly for herbicide use are eliminated by the present invention. The present methodology embodies a sterile production system resulting in grass plantlets that are free of nematodes, pathogenic fungi, and damaging insects. The method of the invention has the capacity to rapidly reproduce and to maintain the genetic purity of promising new cultivars that would otherwise be discarded because they are sterile hybrids, low seed producers, sterile direct DNA transfers, or cross pollinated non-apomicts.

The invention discloses a method for vegetative propagation of an improved thornless rosa rugosa thunb, relates to a method for vegetative propagation of the rosa rugosa thunb, and belongs to the field of flower planting. The method is characterized by comprising the following steps of: short pruning the root of an apricot tree to obtain parental stocks for later use; selecting annual branches with a rosebud as grafting scions; performing pinching when new shoots of scion buds sprout have a length of about 15cm and have 7 to 9 leaves; and performing regular care. The method has the advantages that compared with the culture period of the conventional method, the culture period of the method is obviously shortened; by using the root of the apricot tree, the waste apricot tree are changed into things of value, and the present resources are fully utilized; compared with seedling flowers, the thornless rosa rugosa thunb cultured by the method has rich flower flavor and high content of aromatic oil; and the thornless rosa rugosa thunb cultured by the method is high in resilience, grafted flowers and trees have strong branches, and the thornless roses have high resistance to a lot of diseases and insects, and particularly have obvious resistance to root rot and gray mold.

The invention relates to a rapid propagation method of cassava virus-free seedlings. The method comprises the following steps: step one, collecting explants and carrying out a detoxification pretreatment; step two, disinfecting the surfaces of explants and taking off the stem tips; step three, inducing the stem tips to obtain seedlings; step four, subjecting the seedlings to subculture multiplication culture; step five, carrying out virus detection through a semi-quantitative RT-PCR method; step six, domesticating and transplanting the virus-free tube seedlings. The provided method can effectively remove various viruses and bacteria taking cassava stems as the nutrient source, and obtains virus-free cassava seedlings. The healthy planting, purification and rejuvenation of cassava seedling can be achieved. The occupied space for propagation is small, the propagation speed is quick, and the survival rate of transplanting is high. The provided method can be applied to safe species exchange among different countries and areas, can provide healthy cassava seedlings, reduces the happening and spreading of diseases, and provides technical guarantees for sustainable development of cassava.

A plant sheet aiming at growing a bulky plant such as a succulent plant, which is put into the sheet, at a low cost without injuring the plant. This plant sheet (10) is composed of a network substrate sheet (14), a vegetatively propagating plant to be placed on this substrate sheet (14) and a water-soluble coating for covering the plant and fixing the substrate sheet (14).

The invention discloses a method for establishing a syntrichia caninervis mitt. individual plant cloning system through asexual propagation. The method comprises the following steps: a syntrichia caninervis mitt. pure group is selected, a robustly growing plant is selected and the pure group and the plant are washed and sterilized, then sterilized fine-pointed forceps are used to take the stem tips, stems and stem leaf pieces with strong regeneration capabilities of the treated syntrichia caninervis mitt. individual plant, the stem tips, stems and stem leaf pieces are inoculated to a sterilized BBM solid culture medium and then a culture dish is sealed with a parafilm; the culture dish is put in an illuminating incubator for culture under the conditions that the temperature is 25 DEG C, the illumination intensity is 36mu mol.m<-2>.s<-1> and the light / dark time is 14h / 10h, after culture for 2-3 weeks, new branches will appear on the stem tips, spores and leaf blade bases of the mosses through induction; the protonemata will appear on the stem leaf pieces and new branches will appear when the protonemata are developed to a certain stage; and 2 weeks later, the new branches are takenoff, the stem tips and stems of the new branches are re-transferred to a new solid culture medium and repeated transfer and progressive amplification culture are carried out to form the individual plant cloning system.

The invention belongs to the technical field of ecological environment engineering, and specifically discloses a method for comprehensively treating spartina alterniflora by cutting and ploughing. Themethod comprises the following steps: cutting spartina alterniflora ground plants from a blooming stage to an initial stage of a booting stage for making them unable to produce mature seeds, therebymaking spartina alterniflora unable to perform generative propagation and long-distance diffusion through seeds; in winter, ploughing and breaking roots for the spartina alterniflora which is germinated after being cut, breaking spartina alterniflora stem tubers in a dormant period for making the stem tubers unable to perform vegetative propagation in the next year. The method is simple and easy to implement, effectively blocks sexual reproduction and vegetative reproduction of the spartina alterniflora, is good in treating effect, is lower in cost, is small in influences on environment, can naturally recover beach ecological environment, and is especially suitable for controlling the spartina alterniflora in the cold northern intertidal zone in winter.

Disclosed are a method for controlling flavonoid synthesis in a chrysanthemum plant or non-chrysanthemum plant by genetic recombination technology using a transcriptional regulatory region useful for altering flower color, a method for modifying anthocyanins, a method for producing a chrysanthemum plant or non-chrysanthemum plant containing modified anthocyanins in the petals thereof, and a chrysanthemum plant or non-chrysanthemum plant, progeny thereof, or vegetatively propagated products, part or tissue thereof transformed with the regulatory region. In the method according to the present invention, an expression vector or expression cassette containing a transcriptional regulatory region of perilla anthocyanin 3-acyltransferase gene, such as a nucleic acid containing the nucleotide sequence indicated in SEQ ID NO. 1, or a transcriptional regulatory region of pansy F3′5′H gene, such as the nucleotide sequence indicated in SEQ ID NO. 15, is used.

The invention belongs to the technical field of seed breeding. Japonica rice three-line sterile line plant repeated propagation stock producing method comprises the steps of: (1) individual selection, twin test cross and plant spacing comparison of a maintainer line and a sterile line; (2) plant spacing shed covering or greenhouse propagation of the maintainer line and the sterile line; (3) purity appraisal of the sterile line large samples; (4) open air propagation of the plant of the maintainer line and the sterile line; (5) purity appraisal of the sterile line large samples; (6) storage of the seeds in a refrigeratory of the maintainer line and the sterile line; (7) stock production of the sterile line; (8) purity appraisal of the sterile line large samples. The invention has the advantages of labor saving and time saving, high origin point of the seed purity, increased purity appraisal samples, large propagation coefficient and high production efficiency without purifying year-to-year.