Method for producing original seed for repeat reproduction of japonica rice three-line sterile line strain

Abstract

The invention belongs to the technical field of seed breeding. Japonica rice three-line sterile line plant repeated propagation stock producing method comprises the steps of: (1) individual selection, twin test cross and plant spacing comparison of a maintainer line and a sterile line; (2) plant spacing shed covering or greenhouse propagation of the maintainer line and the sterile line; (3) purity appraisal of the sterile line large samples; (4) open air propagation of the plant of the maintainer line and the sterile line; (5) purity appraisal of the sterile line large samples; (6) storage of the seeds in a refrigeratory of the maintainer line and the sterile line; (7) stock production of the sterile line; (8) purity appraisal of the sterile line large samples. The invention has the advantages of labor saving and time saving, high origin point of the seed purity, increased purity appraisal samples, large propagation coefficient and high production efficiency without purifying year-to-year.

Description

technical field [0001] The invention belongs to the technical field of seed production, in particular to the technical field of three-line hybrid japonica rice seed production. Background technique [0002] my country has about 450 million mu of rice, of which about 130 million mu of japonica rice. Conventional japonica rice and hybrid japonica rice are the two main types of japonica rice production at present, of which conventional japonica rice accounts for about 96% of the total area of ​​japonica rice, while hybrid japonica rice only accounts for 4% of the total area of ​​japonica rice. Poor seed purity and low seed production yield due to imperfect seed production technology is one of the key factors restricting the development of hybrid japonica rice. The production technology of parental stock is the basis of hybrid seed production technology. [0003] Chinese patent 200610010608 discloses the "Three-step Method" for the Purification of the Original Seed of the Dian ...

AI Technical Summary

Problems solved by technology

[0004] This method requires microscopic examination of the pollen of the sterile line at the stage of selecting a single plant, and at the stage of breeding original seeds, it needs to be inspected one by one at the seedling stage, tillering stage, and early ear initial stage, which is time-consuming and labor-intensive, and it is difficult to avoid biological contamination during the breeding process. hybrid and mechanical hybrid

This method needs to use restorer lines to identify recoverability and combining ability in the stage of identifying strains. If there are too few restorer lines, it will be difficult to objectively evaluate the sterile line. If there are too many restorer lines, the workload will be greatly increased.

The original seed produced by this method lacks the link of purity identification, that is, it lacks purity identification in the process of breeding from the original original seed to the original seed, and it is difficult to guarantee the purity of the sterile line parent seeds used for hybrid japonica seed production.

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