20 results about "Maintainer line" patented technology

This invention discloses a rapid method for creating wheat CMS maintainer lines based on recurrent selection and double haploid technology. The method involves crossing a CMS sterile line with a fertile cytoplasmic restorer line to obtain heterozygous fertile plants. Then, using the restorer line as the recurrent paternal parent, a backcross is performed to convert the cytoplasm to a fertile genotype. Double haploid technology is used to rapidly obtain a homozygous DH population from the backcross progeny. Finally, through testcross identification, DH plants with completely sterile cytoplasmic cytoplasm are selected, thus obtaining the target maintainer line with fertile cytoplasm and a sterile nuclear genotype. This invention further incorporates recurrent selection technology for continuous optimization of the maintainer line's agronomic traits. This invention integrates backcrossing, DH technology, and a recurrent selection system, significantly shortening the breeding cycle. The maintainer line exhibits homozygosity ≥99%, a pure cytoplasmic background, strong versatility, and applicability to wheat CMS types, providing efficient technical support for hybrid wheat breeding.

This invention discloses a specific molecular marker for identifying eggplant Saet CMS cytoplasmic male sterility lines and its application. The molecular marker, Saet218b, was selected by comparing the mitochondrial genomes of the eggplant Saet CMS cytoplasmic male sterility line and its maintainer line. Through bioinformatics analysis, the candidate specific gene for Saet CMS male sterility, orf218b, was obtained, and its nucleotide sequence is shown in SEQ ID NO: 1. Based on this sequence information, the molecular marker Saet218b for identifying eggplant Saet CMS cytoplasmic male sterility lines was successfully designed. The molecular marker of this invention can be used to identify eggplant Saet CMS cytoplasmic male sterility lines efficiently and with a simple procedure. The results can be used for marker-assisted breeding of eggplant Saet-derived cytoplasmic male sterility lines, improving breeding efficiency. It also provides technical support and theoretical basis for the identification of eggplant specific cytoplasmic germplasm.

The invention discloses a method for creating a male sterile line and a maintainer line of maize based on a CRISPR-Cas12i. 3 system and application of the method. The invention belongs to the field of genetic breeding, and particularly relates to a method for creating a male sterile line and a maintainer line of maize based on a CRISPR-Cas12i. 3 system and application of the method. The method for preparing the transgenic corn comprises the following steps: mutating an Ms26 gene of a receptor corn genome to cause Ms26 protein function deletion to obtain the transgenic corn, and the transgenic corn has at least one of the following characteristics: (1) compared with the receptor corn, the tassel of the transgenic corn shows compact spikelet, tight glume protection and incapability of normal pollen scattering, and the transgenic corn has the characteristics that the Ms26 protein function deletion is caused by the mutation of the Ms26 gene of the receptor corn genome; no pollen is exposed; and (2) compared with the receptor corn, the transgenic corn is completely aborted. By establishing an efficient backcross transformation method, the genic male sterile line without transgenic ingredients is rapidly obtained, the breeding period is greatly shortened, and the application cost is reduced.

The application belongs to the technical field of bioengineering, and particularly relates to a primer set, a kit and a method for detecting a vector GATV2 transformation event. The primer set comprises left border-specific primers and / or right border-specific primers, the left border-specific primers comprise GATV2-LB-R1 / R2 / R3, and the sequences of the left border-specific primers are shown in SEQ ID NO: 1-3 in sequence respectively; the right border-specific primers comprise GATV2-RB-F1 / F2 / F3, and the sequences of the right border-specific primers are shown in SEQ ID NO: 4-6 in sequence respectively. The primer set of the application can accurately and quickly find the left and right flanking sequences of GATV2, and no specific bands can be amplified in non-transgenic or other transgenic plants, and is suitable for detecting, monitoring and safety management of a rice genetic and artificial technology (GAT) maintainer line GATV2 and a derivative line thereof.

The invention provides a molecular marker for identifying soybean fertility recovery and application thereof. And the fertility recovery sites are Rfa and Rfb. The Rfa marker is located at 32, 913 and 811 positions (reference genome GmaxZH13V2.0) of the No.16 chromosome of the soybean, and is an A / T base mutation site; the Rfb marker is located at 41, 707 and 723 positions (reference genome GmaxZH13V2.0) of the No.9 chromosome of the soybean, and is an A / T base mutation site. GWAS analysis is carried out on the fertility recovery capacity based on 600 maintainer line and restorer line groups, the two sites are identified, and the restorer line and the maintainer line of the soybean germplasm material can be accurately distinguished based on the genotype combination of the two sites. The invention has great theoretical and application values for soybean crossbreeding and related application research thereof.

This invention provides a functional marker primer set for the rice restorer gene Rf4, comprising primer pairs ZcDI-Rf4-MH63-F and ZcDI-Rf4-MH63-R for detecting the H1 haplotype of the Rf4 gene, primer pairs ZcDI-Rf4-ZS97-F and ZcDI-Rf4-ZS97-R for detecting the H4 haplotype of the Rf4 gene, and primer pairs ZcDI-Rf4-Nip-F and ZcDI-Rf4-Nip-R for detecting the H5 haplotype of the Rf4 gene. This invention also provides applications of the functional marker primer set, including one or more of the following applications: detection of the Rf4 haplotype in rice restorer gene; prediction of the restorative properties of rice restorer lines; and breeding of rice restorer lines. This invention designs functional marker primer sets targeting the characteristic recombination sequences of the H1 haplotype with the strongest restoring ability at the Rf4 locus and the H4 and H5 haplotypes with the highest genotype frequencies in the maintainer line. These primer sets are characterized by high specificity and tight linkage, avoiding the drawbacks of designing primers directly inside the functional gene.

The invention provides a functional marker primer group of a rice restorer gene Rf4. The functional marker primer group comprises a primer pair ZcDI-Rf4-MH63-F and a primer pair ZcDI-Rf4-MH63-R for detecting the haplotype of the Rf4 gene H1, a primer pair ZcDI-Rf4-ZS97-F and a primer pair ZcDI-Rf4-ZS97-R for detecting the haplotype of the Rf4 gene H4, and a primer pair ZcDI-Rf4-Nip-F and a primer pair ZcDI-Rf4-Nip-R for detecting the haplotype of the Rf4 gene H5. The invention also provides an application of the functional marker primer group. The functional marker primer group comprises more than one of the following applications: detection of rice restorer gene Rf4 haplotype; the method is used for predicting the restorability of the rice restorer; the method is used for rice restorer line breeding. The functional marker primer group is designed by taking the characteristic recombinant sequences of the H1 haplotype with the strongest restoring force on the locus of the restoring gene Rf4 and the H4 and H5 haplotypes with the highest genotype frequency in the maintainer line as targets, has the characteristics of high specificity and tight linkage, and avoids the defect that primers are directly designed in functional genes.

The invention discloses a high-quality broom sorghum variety breeding method, which comprises the following steps: parent matching and hybridization, filial generation screening and character directional cultivation, quality detection and matched cultivation verification, selection of a grain maintainer line and a broom restorer line for hybridization, selection of a broom maintainer line from the offspring, continuous backcross of the broom maintainer line and a sterile line, and breeding of the broom sorghum variety. A sterile line for brooms, a sterile line for brooms and a plurality of restorer lines are created for test mating, finally, the obtained F1 seeds are planted according to a wide-row close planting method, the row spacing is 60-70 cm, the double-row spacing on ridges is 20 cm, 6000-7000 seedlings are kept per mu, decomposed farmyard manure is applied to base fertilizer, and compound fertilizer is applied to base fertilizer; 10-15 kg / mu of urea is topdressed in the jointing stage, and water is controlled in the wax ripening stage until the soil water content is smaller than or equal to 15%, so that the high-quality broom sorghum with high branch strength, high disease resistance and high lodging resistance is obtained.

The present invention relates to the field of molecular biology technology, and specifically to a SNP molecular marker, primer set, application and method of the Brassica napus Polima cytoplasmic sterility gene. The SNP molecular marker is BN9000345. The primer set includes BN9000345_K01_X, BN9000345_K01_Y and BN9000345_K01_C. The application is the use of the SNP molecular marker in detecting whether Brassica napus is of the Polima sterile cytoplasmic type. The method is used to determine whether Brassica napus is of the Polima sterile cytoplasmic type. The present invention can quickly and accurately detect whether rapeseed contains the Brassica napus Polima cytoplasmic male sterility gene, and is suitable for large-scale and high-throughput Brassica napus Polima cytoplasmic male sterility gene identification (such as purity identification of sterile line and maintainer line parents) and screening.

The present invention belongs to the field of plant genetic engineering. Provided is a gene ZmKO1 for regulating flowering and pollen shedding of a maize tassel. Mutating the gene ZmKO1 can lead to a genic male sterility phenomenon. The breeding of a maize genic male sterile line can be achieved on the basis of the gene ZmKO1 site using the CRISPR-Cas9 technology or mutant gene backcross breeding technology. In the silking stage of the sterile line, spraying exogenous gibberellin on the tassel can restore fertility in male flowers of the sterile line, enabling normal self-pollination and reproduction, thereby achieving an economical and efficient maintenance of the sterile line as a maintainer line. The genic male sterile line created on the basis of the ZmKO1 gene site has the characteristic of one line for two uses, and can be used for maize male sterile hybrid seed production. Further provided is a technical system for achieving maize two-line sterile hybrid seed production using ZmKO1 gene mutations.

The application discloses a rice stigma width regulation gene qSTB-8 and application of an encoded protein thereof, a nucleotide sequence of the rice stigma width regulation gene qSTB-8 is shown as SEQ ID NO. 1, and the application is selected from any one or two or more of the following: (1) application in regulation of rice stigma width; (2) application in improvement of rice stigma exposure rate breeding; (3) application in improvement of rice cross-pollination rate breeding. The application is configured by hybridization of a wide-stigma indica rice maintainer line Chuan 345B and a narrow-stigma high-quality indica rice maintainer line Chuan 106B into a BC4F5 positioning population, then a method of map-based cloning is used, SSR molecular markers are used for preliminary positioning, and finally qSTB-8 is finely positioned in a 11.2 kb segment between markers SG930 and SG950. Gene function analysis of qSTB-8 shows that a transgenic line Chuan 106B has a wide-stigma trait.

The invention discloses an alfalfa seed specific promoter and application thereof in intelligent maintainer line cultivation. The invention belongs to the technical field of biology, and particularly relates to an alfalfa seed specific promoter and application thereof in intelligent maintainer line cultivation. The invention provides a DNA molecule a), b) or c) as follows: a) a DNA molecule at the 25th-2210th site of which the nucleotide sequence is SEQ ID No: 1; b) a DNA fragment which has more than 99%, more than 95%, more than 90%, more than 85% or more than 80% of identity with the nucleotide sequence limited by the a) and is derived from the same function; and c) a DNA fragment which is hybridized with the nucleotide sequence limited by a) or b) under strict conditions and has a promoter function. The alfalfa seed specific promoter OLEpro provided by the invention can be used for improving the expression of a gene in seed specificity, and is applied to plant seed specific marking or seed character improvement breeding.

The present application relates to rice breeding technology field, and provides a high alkali consumption value Guangxi Zhuang autonomous region rice three-line sterile line breeding method, which comprises the following steps: taking a high alkali consumption value variety in Guangxi Zhuang autonomous region as a male parent, taking a three-line maintainer line rice variety as a female parent, hybridizing to obtain F1 generation; backcrossing the female parent with F1 generation to obtain BC1F1 generation; continuously backcrossing to obtain BC5F1 generation, selfing to obtain BC5F2 generation; continuously selfing BC5F2 generation to obtain a three-line maintainer line; hybridizing the three-line maintainer line as a male parent with a three-line sterile line rice variety to obtain a sterile line rice; backcrossing the sterile line rice with the three-line maintainer line to obtain backcross progeny; taking the three-line maintainer line as a recurrent male parent, selecting a target plant in the backcross progeny for continuous backcrossing to obtain a high alkali consumption value rice three-line sterile line. The method is simple and easy to operate, and provides an excellent rice three-line sterile line. It can be used for rice improvement and quality improvement of rice.

The invention relates to the technical field of chemically induced male sterility, in particular to a chemically induced male sterility method and application thereof. According to the method disclosed by the invention, a fixed sterile line, a maintainer line and a fixed restorer line are not provided, the non-hereditary male sterile line is generated only by carrying out chemical induced sterility on any parent inbred line in the excellent hybrid combination, and the hybrid can be prepared by taking the other parent inbred line as a pollination line; the chemically induced male sterile line (chemically killed line) can be used for preparing a heterozygous disease-resistant hybrid with a single dominant disease-resistant gene (containing a clubroot disease-resistant gene) and preparing a high-oil and high-yield hybrid with oil content, thousand seed weight, seed number and the like of rape with quantitative inheritable characters.

The invention relates to a mutant protein related to rice female sterility, a gene and a molecular marker and application thereof. The 1106th nucleotide of the rice LOCOs12g38460 gene is mutated from A to G, a mutant h569 with normal male fertility and a female sterile phenotype is obtained, and the female sterile phenotype of the mutant is caused by embryo sac abortion due to the fact that macrospore mother cells of the mutant cannot enter meiosis. The Guangdong three-line female sterile maintainer line constructed by using the mutant can solve the seed reproduction problem of the female sterile mutant. The method can be applied to hybrid rice seed production, so that the work of manually cutting off male parents after male and female parent row-by-row planting and pollination is omitted, the current seed production process which is complicated in process and wastes time and labor is changed, hybrid rice male and female parent mixed sowing and mixed harvesting whole-process mechanized seed production is realized, and the method has very important practical significance on agricultural production.

The invention relates to a method for breeding hybrid rice with high seed production purity, which comprises the following steps: hybridizing a two-line sterile line and a three-line maintainer line, and breeding a new two-line sterile line with a three-line maintainer gene target character in a pedigree selection mode; the three-line sterile line and the new two-line sterile line are respectively subjected to test cross to obtain an F1 generation, new two-line sterile line strains with maintainer sterile genes of the three-line sterile line are determined to be subjected to continuous backcross and selfing, and a new three-line sterile line with three-line maintainer line characters and a new two-line sterile line with maintainer genes are obtained through breeding. The new rice three-line sterile line and the new two-line sterile line bred by the method can be respectively hybridized with a restoring line to produce hybrids, the purity of the three-line hybrids is improved (99.0% or above), meanwhile, the risk of low-temperature transformation and quotient transformation in the production of the two-line hybrids is greatly reduced, high-purity hybrid rice seeds are obtained, and the method is suitable for large-scale popularization and application. The commodity of the hybrid rice seeds is well guaranteed and improved.

The invention discloses a method for breeding a long-grain aromatic indica rice two-line sterile line. The method comprises the following steps: hybridizing a two-line sterile material HD-37S and an improved maintainer line (Taifeng B / Guangdong 8B) F3 as parents, carrying out multi-generation pedigree breeding, combining with molecular marker-assisted selection aiming at a fragrance gene fgr and a grain length gene gw7, and carrying out shuttle fertility identification in high altitude of Guangxi and Hainan region, so as to finally screen out a stable sterile line of which the pollen sterility is greater than or equal to 99.9%. According to the sterile line bred by the method, parent sources are HD-37S and (Taifeng B / Guangxi 8B) F3, and the sterile line has excellent characters that the grain length is greater than or equal to 7.0 mm, the length-width ratio is greater than or equal to 4.0, and the fragrance score is greater than or equal to 75. According to the method, efficient polymerization of long grains, fragrance and stable sterility is realized, the breeding period is short, and the selection accuracy is high.

The invention discloses a three-line crossbreeding method for clustered pod pepper, which comprises the following steps: breeding by using a three-line method, the three-line method is characterized in that a three-line crossbreeding matching system is carried out by using a sterile line, a maintainer line and a restorer line, the restorer line is used as a male parent, the sterile line is used as a female parent, hybridization is carried out, and seeds harvested on sterile plants are hybrid seeds; the maintainer line is used as a male parent, the sterile line is used as a female parent, hybridization is performed, seeds harvested on the sterile line are still the sterile line, and the sterile line continues to breed.

This application discloses a rapid detection method for the genetic purity of BT-type cytoplasmic male sterile rice seeds based on real-time quantitative PCR. The method primarily utilizes specific primers designed targeting sequences unique to maintainer lines in the rice mitochondrial genome, using the rice internal reference gene ATP6 as a reference to ensure high specificity and accuracy. A standard curve of a mixture of sterile and maintainer line seeds constructed based on this method allows the conversion of ΔCt values ​​into relative abundance for quantitative analysis. Results show that this method exhibits extremely strong linearity, accurate quantification in simulated mixed samples, and a near 100% recovery rate, providing a rapid, stable, and reliable detection method for the quality control of rice hybrids.

The application discloses a method for cultivating saline-alkali tolerant hybrid barley seeds based on barley three-line method, takes a barley male sterile line parent 270A as a sterile source, takes a known saline-alkali tolerant variety Yanshima No. 11 as a recurrent parent and a maintainer line, introduces a major saline-alkali QTL site carried by Yanshima No. 11 into the sterile line background through continuous multiple generations of backcrossing, simultaneously fixes the agronomic traits, and finally breeds a stable saline-alkali tolerant male sterile line Yanshima No. 16; then takes Yanshima No. 11 as the maintainer line, plants parent and offspring in a 2:1 ratio in a saline-alkali stress field, implements artificial assisted pollination and dynamic hybrid removal during the heading stage to the flowering stage, and harvests seeds formed by sterile line plants, so that saline-alkali tolerant hybrid barley F1 generation seeds are obtained. The application realizes precise coupling of the saline-alkali tolerance of barley and the three-line matching system for the first time, overcomes the bottleneck of long breeding period and difficult utilization of hybrid vigor in traditional saline-alkali tolerance breeding, and the F1 seeds produced have a yield increase of 23.6% under saline-alkali soil compared with conventional varieties.