6 results about "Maintainer line" patented technology

This invention discloses a rapid method for creating wheat CMS maintainer lines based on recurrent selection and double haploid technology. The method involves crossing a CMS sterile line with a fertile cytoplasmic restorer line to obtain heterozygous fertile plants. Then, using the restorer line as the recurrent paternal parent, a backcross is performed to convert the cytoplasm to a fertile genotype. Double haploid technology is used to rapidly obtain a homozygous DH population from the backcross progeny. Finally, through testcross identification, DH plants with completely sterile cytoplasmic cytoplasm are selected, thus obtaining the target maintainer line with fertile cytoplasm and a sterile nuclear genotype. This invention further incorporates recurrent selection technology for continuous optimization of the maintainer line's agronomic traits. This invention integrates backcrossing, DH technology, and a recurrent selection system, significantly shortening the breeding cycle. The maintainer line exhibits homozygosity ≥99%, a pure cytoplasmic background, strong versatility, and applicability to wheat CMS types, providing efficient technical support for hybrid wheat breeding.

This invention discloses a specific molecular marker for identifying eggplant Saet CMS cytoplasmic male sterility lines and its application. The molecular marker, Saet218b, was selected by comparing the mitochondrial genomes of the eggplant Saet CMS cytoplasmic male sterility line and its maintainer line. Through bioinformatics analysis, the candidate specific gene for Saet CMS male sterility, orf218b, was obtained, and its nucleotide sequence is shown in SEQ ID NO: 1. Based on this sequence information, the molecular marker Saet218b for identifying eggplant Saet CMS cytoplasmic male sterility lines was successfully designed. The molecular marker of this invention can be used to identify eggplant Saet CMS cytoplasmic male sterility lines efficiently and with a simple procedure. The results can be used for marker-assisted breeding of eggplant Saet-derived cytoplasmic male sterility lines, improving breeding efficiency. It also provides technical support and theoretical basis for the identification of eggplant specific cytoplasmic germplasm.

The present invention belongs to the field of plant genetic engineering. Provided is a gene ZmKO1 for regulating flowering and pollen shedding of a maize tassel. Mutating the gene ZmKO1 can lead to a genic male sterility phenomenon. The breeding of a maize genic male sterile line can be achieved on the basis of the gene ZmKO1 site using the CRISPR-Cas9 technology or mutant gene backcross breeding technology. In the silking stage of the sterile line, spraying exogenous gibberellin on the tassel can restore fertility in male flowers of the sterile line, enabling normal self-pollination and reproduction, thereby achieving an economical and efficient maintenance of the sterile line as a maintainer line. The genic male sterile line created on the basis of the ZmKO1 gene site has the characteristic of one line for two uses, and can be used for maize male sterile hybrid seed production. Further provided is a technical system for achieving maize two-line sterile hybrid seed production using ZmKO1 gene mutations.

The application discloses a rice stigma width regulation gene qSTB-8 and application of an encoded protein thereof, a nucleotide sequence of the rice stigma width regulation gene qSTB-8 is shown as SEQ ID NO. 1, and the application is selected from any one or two or more of the following: (1) application in regulation of rice stigma width; (2) application in improvement of rice stigma exposure rate breeding; (3) application in improvement of rice cross-pollination rate breeding. The application is configured by hybridization of a wide-stigma indica rice maintainer line Chuan 345B and a narrow-stigma high-quality indica rice maintainer line Chuan 106B into a BC4F5 positioning population, then a method of map-based cloning is used, SSR molecular markers are used for preliminary positioning, and finally qSTB-8 is finely positioned in a 11.2 kb segment between markers SG930 and SG950. Gene function analysis of qSTB-8 shows that a transgenic line Chuan 106B has a wide-stigma trait.

This application discloses a rapid detection method for the genetic purity of BT-type cytoplasmic male sterile rice seeds based on real-time quantitative PCR. The method primarily utilizes specific primers designed targeting sequences unique to maintainer lines in the rice mitochondrial genome, using the rice internal reference gene ATP6 as a reference to ensure high specificity and accuracy. A standard curve of a mixture of sterile and maintainer line seeds constructed based on this method allows the conversion of ΔCt values ​​into relative abundance for quantitative analysis. Results show that this method exhibits extremely strong linearity, accurate quantification in simulated mixed samples, and a near 100% recovery rate, providing a rapid, stable, and reliable detection method for the quality control of rice hybrids.

The application discloses a method for cultivating saline-alkali tolerant hybrid barley seeds based on barley three-line method, takes a barley male sterile line parent 270A as a sterile source, takes a known saline-alkali tolerant variety Yanshima No. 11 as a recurrent parent and a maintainer line, introduces a major saline-alkali QTL site carried by Yanshima No. 11 into the sterile line background through continuous multiple generations of backcrossing, simultaneously fixes the agronomic traits, and finally breeds a stable saline-alkali tolerant male sterile line Yanshima No. 16; then takes Yanshima No. 11 as the maintainer line, plants parent and offspring in a 2:1 ratio in a saline-alkali stress field, implements artificial assisted pollination and dynamic hybrid removal during the heading stage to the flowering stage, and harvests seeds formed by sterile line plants, so that saline-alkali tolerant hybrid barley F1 generation seeds are obtained. The application realizes precise coupling of the saline-alkali tolerance of barley and the three-line matching system for the first time, overcomes the bottleneck of long breeding period and difficult utilization of hybrid vigor in traditional saline-alkali tolerance breeding, and the F1 seeds produced have a yield increase of 23.6% under saline-alkali soil compared with conventional varieties.