12 results about "Maintainer line" patented technology

This invention discloses a rapid method for creating wheat CMS maintainer lines based on recurrent selection and double haploid technology. The method involves crossing a CMS sterile line with a fertile cytoplasmic restorer line to obtain heterozygous fertile plants. Then, using the restorer line as the recurrent paternal parent, a backcross is performed to convert the cytoplasm to a fertile genotype. Double haploid technology is used to rapidly obtain a homozygous DH population from the backcross progeny. Finally, through testcross identification, DH plants with completely sterile cytoplasmic cytoplasm are selected, thus obtaining the target maintainer line with fertile cytoplasm and a sterile nuclear genotype. This invention further incorporates recurrent selection technology for continuous optimization of the maintainer line's agronomic traits. This invention integrates backcrossing, DH technology, and a recurrent selection system, significantly shortening the breeding cycle. The maintainer line exhibits homozygosity ≥99%, a pure cytoplasmic background, strong versatility, and applicability to wheat CMS types, providing efficient technical support for hybrid wheat breeding.

This invention discloses a specific molecular marker for identifying eggplant Saet CMS cytoplasmic male sterility lines and its application. The molecular marker, Saet218b, was selected by comparing the mitochondrial genomes of the eggplant Saet CMS cytoplasmic male sterility line and its maintainer line. Through bioinformatics analysis, the candidate specific gene for Saet CMS male sterility, orf218b, was obtained, and its nucleotide sequence is shown in SEQ ID NO: 1. Based on this sequence information, the molecular marker Saet218b for identifying eggplant Saet CMS cytoplasmic male sterility lines was successfully designed. The molecular marker of this invention can be used to identify eggplant Saet CMS cytoplasmic male sterility lines efficiently and with a simple procedure. The results can be used for marker-assisted breeding of eggplant Saet-derived cytoplasmic male sterility lines, improving breeding efficiency. It also provides technical support and theoretical basis for the identification of eggplant specific cytoplasmic germplasm.

The invention discloses a method for creating a male sterile line and a maintainer line of maize based on a CRISPR-Cas12i. 3 system and application of the method. The invention belongs to the field of genetic breeding, and particularly relates to a method for creating a male sterile line and a maintainer line of maize based on a CRISPR-Cas12i. 3 system and application of the method. The method for preparing the transgenic corn comprises the following steps: mutating an Ms26 gene of a receptor corn genome to cause Ms26 protein function deletion to obtain the transgenic corn, and the transgenic corn has at least one of the following characteristics: (1) compared with the receptor corn, the tassel of the transgenic corn shows compact spikelet, tight glume protection and incapability of normal pollen scattering, and the transgenic corn has the characteristics that the Ms26 protein function deletion is caused by the mutation of the Ms26 gene of the receptor corn genome; no pollen is exposed; and (2) compared with the receptor corn, the transgenic corn is completely aborted. By establishing an efficient backcross transformation method, the genic male sterile line without transgenic ingredients is rapidly obtained, the breeding period is greatly shortened, and the application cost is reduced.

The application belongs to the technical field of bioengineering, and particularly relates to a primer set, a kit and a method for detecting a vector GATV2 transformation event. The primer set comprises left border-specific primers and / or right border-specific primers, the left border-specific primers comprise GATV2-LB-R1 / R2 / R3, and the sequences of the left border-specific primers are shown in SEQ ID NO: 1-3 in sequence respectively; the right border-specific primers comprise GATV2-RB-F1 / F2 / F3, and the sequences of the right border-specific primers are shown in SEQ ID NO: 4-6 in sequence respectively. The primer set of the application can accurately and quickly find the left and right flanking sequences of GATV2, and no specific bands can be amplified in non-transgenic or other transgenic plants, and is suitable for detecting, monitoring and safety management of a rice genetic and artificial technology (GAT) maintainer line GATV2 and a derivative line thereof.

The invention provides a molecular marker for identifying soybean fertility recovery and application thereof. And the fertility recovery sites are Rfa and Rfb. The Rfa marker is located at 32, 913 and 811 positions (reference genome GmaxZH13V2.0) of the No.16 chromosome of the soybean, and is an A / T base mutation site; the Rfb marker is located at 41, 707 and 723 positions (reference genome GmaxZH13V2.0) of the No.9 chromosome of the soybean, and is an A / T base mutation site. GWAS analysis is carried out on the fertility recovery capacity based on 600 maintainer line and restorer line groups, the two sites are identified, and the restorer line and the maintainer line of the soybean germplasm material can be accurately distinguished based on the genotype combination of the two sites. The invention has great theoretical and application values for soybean crossbreeding and related application research thereof.

This invention provides a functional marker primer set for the rice restorer gene Rf4, comprising primer pairs ZcDI-Rf4-MH63-F and ZcDI-Rf4-MH63-R for detecting the H1 haplotype of the Rf4 gene, primer pairs ZcDI-Rf4-ZS97-F and ZcDI-Rf4-ZS97-R for detecting the H4 haplotype of the Rf4 gene, and primer pairs ZcDI-Rf4-Nip-F and ZcDI-Rf4-Nip-R for detecting the H5 haplotype of the Rf4 gene. This invention also provides applications of the functional marker primer set, including one or more of the following applications: detection of the Rf4 haplotype in rice restorer gene; prediction of the restorative properties of rice restorer lines; and breeding of rice restorer lines. This invention designs functional marker primer sets targeting the characteristic recombination sequences of the H1 haplotype with the strongest restoring ability at the Rf4 locus and the H4 and H5 haplotypes with the highest genotype frequencies in the maintainer line. These primer sets are characterized by high specificity and tight linkage, avoiding the drawbacks of designing primers directly inside the functional gene.

The present invention belongs to the field of plant genetic engineering. Provided is a gene ZmKO1 for regulating flowering and pollen shedding of a maize tassel. Mutating the gene ZmKO1 can lead to a genic male sterility phenomenon. The breeding of a maize genic male sterile line can be achieved on the basis of the gene ZmKO1 site using the CRISPR-Cas9 technology or mutant gene backcross breeding technology. In the silking stage of the sterile line, spraying exogenous gibberellin on the tassel can restore fertility in male flowers of the sterile line, enabling normal self-pollination and reproduction, thereby achieving an economical and efficient maintenance of the sterile line as a maintainer line. The genic male sterile line created on the basis of the ZmKO1 gene site has the characteristic of one line for two uses, and can be used for maize male sterile hybrid seed production. Further provided is a technical system for achieving maize two-line sterile hybrid seed production using ZmKO1 gene mutations.

The application discloses a rice stigma width regulation gene qSTB-8 and application of an encoded protein thereof, a nucleotide sequence of the rice stigma width regulation gene qSTB-8 is shown as SEQ ID NO. 1, and the application is selected from any one or two or more of the following: (1) application in regulation of rice stigma width; (2) application in improvement of rice stigma exposure rate breeding; (3) application in improvement of rice cross-pollination rate breeding. The application is configured by hybridization of a wide-stigma indica rice maintainer line Chuan 345B and a narrow-stigma high-quality indica rice maintainer line Chuan 106B into a BC4F5 positioning population, then a method of map-based cloning is used, SSR molecular markers are used for preliminary positioning, and finally qSTB-8 is finely positioned in a 11.2 kb segment between markers SG930 and SG950. Gene function analysis of qSTB-8 shows that a transgenic line Chuan 106B has a wide-stigma trait.

The invention discloses an alfalfa seed specific promoter and application thereof in intelligent maintainer line cultivation. The invention belongs to the technical field of biology, and particularly relates to an alfalfa seed specific promoter and application thereof in intelligent maintainer line cultivation. The invention provides a DNA molecule a), b) or c) as follows: a) a DNA molecule at the 25th-2210th site of which the nucleotide sequence is SEQ ID No: 1; b) a DNA fragment which has more than 99%, more than 95%, more than 90%, more than 85% or more than 80% of identity with the nucleotide sequence limited by the a) and is derived from the same function; and c) a DNA fragment which is hybridized with the nucleotide sequence limited by a) or b) under strict conditions and has a promoter function. The alfalfa seed specific promoter OLEpro provided by the invention can be used for improving the expression of a gene in seed specificity, and is applied to plant seed specific marking or seed character improvement breeding.

The invention discloses a method for breeding a long-grain aromatic indica rice two-line sterile line. The method comprises the following steps: hybridizing a two-line sterile material HD-37S and an improved maintainer line (Taifeng B / Guangdong 8B) F3 as parents, carrying out multi-generation pedigree breeding, combining with molecular marker-assisted selection aiming at a fragrance gene fgr and a grain length gene gw7, and carrying out shuttle fertility identification in high altitude of Guangxi and Hainan region, so as to finally screen out a stable sterile line of which the pollen sterility is greater than or equal to 99.9%. According to the sterile line bred by the method, parent sources are HD-37S and (Taifeng B / Guangxi 8B) F3, and the sterile line has excellent characters that the grain length is greater than or equal to 7.0 mm, the length-width ratio is greater than or equal to 4.0, and the fragrance score is greater than or equal to 75. According to the method, efficient polymerization of long grains, fragrance and stable sterility is realized, the breeding period is short, and the selection accuracy is high.

This application discloses a rapid detection method for the genetic purity of BT-type cytoplasmic male sterile rice seeds based on real-time quantitative PCR. The method primarily utilizes specific primers designed targeting sequences unique to maintainer lines in the rice mitochondrial genome, using the rice internal reference gene ATP6 as a reference to ensure high specificity and accuracy. A standard curve of a mixture of sterile and maintainer line seeds constructed based on this method allows the conversion of ΔCt values ​​into relative abundance for quantitative analysis. Results show that this method exhibits extremely strong linearity, accurate quantification in simulated mixed samples, and a near 100% recovery rate, providing a rapid, stable, and reliable detection method for the quality control of rice hybrids.

The application discloses a method for cultivating saline-alkali tolerant hybrid barley seeds based on barley three-line method, takes a barley male sterile line parent 270A as a sterile source, takes a known saline-alkali tolerant variety Yanshima No. 11 as a recurrent parent and a maintainer line, introduces a major saline-alkali QTL site carried by Yanshima No. 11 into the sterile line background through continuous multiple generations of backcrossing, simultaneously fixes the agronomic traits, and finally breeds a stable saline-alkali tolerant male sterile line Yanshima No. 16; then takes Yanshima No. 11 as the maintainer line, plants parent and offspring in a 2:1 ratio in a saline-alkali stress field, implements artificial assisted pollination and dynamic hybrid removal during the heading stage to the flowering stage, and harvests seeds formed by sterile line plants, so that saline-alkali tolerant hybrid barley F1 generation seeds are obtained. The application realizes precise coupling of the saline-alkali tolerance of barley and the three-line matching system for the first time, overcomes the bottleneck of long breeding period and difficult utilization of hybrid vigor in traditional saline-alkali tolerance breeding, and the F1 seeds produced have a yield increase of 23.6% under saline-alkali soil compared with conventional varieties.