474 results about "Maintainer line" patented technology

The invention discloses a cultivation method of a rice engineering maintainer line and a method for applying the cultivated rice engineering maintainer line to breeding of a rice genic male sterile line. The cultivation method comprises the following steps: firstly, positioning and cloning a male sterile gene s of the rice genic male sterile line and a fertile gene S of a male sterile line source parent through a map position cloning method; connecting a color marker gene C and the fertile gene S to the same dual expression vector to enable the color marker gene C and the fertile gene S to be subjected to linkage inheritance and coseparation after being introduced into a plant; transferring the expression vector in the genic male sterile line by a trangenosis method to obtain a fertility restorer strain; and then carrying out back-crossing on the fertility restorer strain and the genic male sterile line to obtain the engineering maintainer line. The cultivation method can be applied to breeding of the rice genic male sterile line, and can simplify the breeding process of the genic male line greatly.

The invention discloses a method for creating rice engineering maintainer lines preventive against gene flow. The method includes the steps of 1, site-specifically inserting an exogenous reporter gene and a pollen lethal gene into a flanking sequence of a fertility control gene of a wild rice chromosome so as to obtain a transgenic line containing the exogenous reporter gene and the pollen lethal gene, wherein the exogenous reporter gene, the pollen lethal gene and the fertility control gene are able to closely link and inherit; 2, hybridizing wild rice and homozygous recessive rice common genic male sterile lines to obtain F1-generation plants, hybridizing the F1-generation plants as male parents to the transgenic line to obtain a generation F2, screening the generation F2 to obtain hybrid lines containing the exogenous reporter gene, namely rice engineering maintainer lines required, wherein the homozygous recessive rice common genic male sterile lines are mutant lines with the sterility control gene. The invention further discloses application of the rice engineering maintainer lines preventive against gene flow in rice breeding.

Disclosed is a three-line method for mechanical production of hybrid rice seeds. The method is characterized in that a rice variety with hull color different from normal hull color (abnormal color) is selected to hybridize with a maintainer line rice variety with normal hull color so as to obtain maintainer line rice seeds with abnormal hull color, and trans-breeding is performed to obtain sterile line rice seeds with abnormal hull color; and that the obtained sterile line rice seeds with abnormal hull color and restorer line rice seeds with normal hull color are mechanically sown in a mixed manner, mechanical pollen shedding is carried out during the blooming stage, hybrid rice seeds with abnormal hull color and restorer line rice seeds with normal hull color obtained by rice selfing are mechanically harvested, and hybrid seeds and restorer line seeds are separated by use of a color sorter. The method of the invention can be applied to hybrid rice seed production.

The invention relates to the field of plant breeding, and particularly relates to the fertility restoration of a homozygous recessive genic male sterility rice plant. Furthermore, the invention relates to a method for establishing a plant male sterile line and a maintainer line and a genetic transformation material, and more particularly relates to a structure, a rice cell, tissue or organ, a method for establishing a rice male sterile line and a maintainer line, a method for restoring the male fertility of a rice sterile plant, a method for preparing rice seeds, a genetic transformation material, a method for preparing hybrid rice and an application of the rice male sterile line in preparation of hybrid rice.

This invention relates to a nuclear conditional male sterility system in Brassica napus. Embodiments of the invention provide for the (male sterile) prebasic female (MsMsrfrf), the (male fertile) maintainer line (msmsrfrf), the (male sterile) basic female line (Msmsrfrf), and hybrid lines. Further provided are methods for the production of those lines. Further embodiments of the invention relate to markers associated to the sterility, fertility and maintainer alleles and the use of those markers in providing a hybrid system.

The invention relates to a cultivating method of nuclear male sterile hybrid wheat, belonging to the field of crop cultivation. Prior method adds the 4E chromosome of Agropyron elongatum to a recessive genic male sterile wheat; the seed set of the acquired blue marked wheat genic male sterile maintainer line ranges from 40% to 50%; the white male sterile seed separated from self-crossed progeny is only around 64%. The invention is characterized in that, hybridization is carried out by taking lanzhou genic male sterile wheat as female parent and Agropyron elongatum as male parent; through light blue line self bred or white, light blue and dark blue three-color mixed line plant mixing (inbred + outcross), white sterile line and light blue fertile line seeds are bred; a photoelectric color sorter is used to sort the bred and produced white seeds and blue seeds, and in the blue seed group, the dark blue seeds are sorted and eliminated as much as possible; white sterile line and male parent line are hybrid to produce commercial hybrid F1 seeds. The cultivating method has the advantages that, the acquired light blue line has a high self seed set above 85 %, and the self seed set of white sterile line seeds can achieve around 80%.

The invention belongs to the technical field of rape breeding, and discloses a selection breeding method for a Brassica napus-turnip cabbage cytoplasmic male sterility line (CMS). The method is characterized in that an artificially-bred turnip cabbage allotetraploid as a female parent is hybridized with a Brassica napus variety as a male parent, male sterile plants are isolated from the progeny of hybrid selfing, and a Brassica napus-turnip cabbage cytoplasmic male sterility line with genetic stability is obtained by successive backcross breeding. Restorer line and maintainer line relationship and molecular biological methods are employed to prove genetically and on DNA level respectively that the Brassica napus-turnip cabbage cytoplasmic male sterility line has a sterile cytoplasmic type different from those of Polima cytoplasmic male sterility (pol CMS), turnip cytoplasmic male sterility (ogu CMS, kos CMS) and Brassica juncea cytoplasmic male sterility (hau CMS). The invention adds a new rape cytoplasmic male sterility type for China and the rest of the world.

The invention discloses a method for breeding a recessive nuclear sterile temporary maintainer line by utilizing a Brassica napus microspore culture technology, which comprises the following steps of: crossing by taking a good variety/strain as a female parent and taking a recessive nuclear sterile temporary maintainer line as a male parent to obtain an F1 generation; then carrying out inducing culture on the isolated microspore of the plant of the F1 generation by a Brassica napus microspore culture technology, thereby obtaining a DH strain; carrying out self cross on the DH strain and carrying out test cross on the heat exchange and a sterile line, wherein the test cross variety is completely sterile, and the corresponding DH strain is the recessive nuclear sterile temporary maintainer line. The good temporary maintainer line with a good genetic background can be rapidly obtained by the method and is applied to Brassica napus recessive nuclear sterile cross breeding.

The invention relates to a method for breeding kenaf H040 cytoplasmic male sterile line, and belongs to the technical field of breeding of crop male sterile lines. The method takes kenaf wild species H040 or filial generation inherited with cytoplasm of the kenaf wild species H040 as female parent, and takes UG 93 cytoplasmic male sterile maintainer line or kenaf common culture variety as male parent; the female parent and the male parent carry out saturated backcross; the H040 cytoplasmic male sterile line, which is similar to the male parent and is orderly and consistent and exhibits male sterility, is selected and bred; and the backcross female parent is the male sterile maintainer line. The method provides new source of the cytoplasm for the breeding of the kenaf male sterile line, and can combine hybridized combination with comparative advantages.

The invention discloses a method for transforming a hot-pepper nucleo-cytoplasmic interactive male-sterile line, a homo-maintainer line and a homo-restorer line, which comprises the following steps of: hybridizing by using a hot-pepper selfing line as a male parent and the hot-pepper nucleo-cytoplasmic interactive male-sterile line as a female parent, simultaneously selfing a male parent plant, selecting sterile plants or other inflorescences in 4-50 corresponding male-parent filial single plants and hot-pepper filial generations for paired test crossing when in filial-generation fertility separation, selfing each male-parent single plant, and respectively processing according to the group fertility expression of test crossing generations to select the male-sterile line, the homo-maintainer line and the homo-restorer line. When in filial-generation group fertility separation, the invention not only can be used for transforming the hot-pepper nucleo-cytoplasmic interactive male-sterile line and the homo-maintainer line from the hot-pepper selfing line, but also can be used for breeding the homo-restorer line, expands the selecting space of the restored male parents of other homologous sterile lines and can be used for screening more excellent three-line combined varieties. A hot-pepper hybrid is produced by adopting a male-sterile three-line technology, all the complex pollination procedures are simplified, and the economic benefit is quite remarkable.

The invention discloses a selecting and breeding method for long-grained hybrid japonica rice. The method comprises the following steps of: (1) selecting a long-grained type rice resource material as a donor parent; (2) respectively hybridizing the donor parent carried with long-grained type gene and the traditional round-grained japonica rice restorer line and japonica rice maintainer line; (3) in the separation process of offspring, selecting seeds with rice grain length greater than or equal to 6.5mm harvested from single plants; (4) performing test mating on the stabled long-grained type material obtained from the step (3) with a japonica rice sterile line, and selecting to obtain a long-grained restorer line and a long-grained japonica rice sterile line; and (6) performing test mating on the long-grained japonica rice sterile line obtained from the step (5) with the long-grained restorer line obtained from the step (4), and preparing a new long-grained hybrid japonica rice variety. The selecting and breeding method provided by the invention is applied to grain type improvement on the existing round-grained hybrid japonica rice, creates the long-grained type hybrid japonica rice and obviously improves appearance and quality of rice.

The invention discloses a plant hybridization system and application. The plant hybridization system consists of a sterile line, a maintainer line and a restorer line; the maintainer line contains a TWSD carrier, and the TWSD carrier at least simultaneously contains five closely linked expression frames, including the herbicide resistance gene expression frame, the plant endogenous herbicide resistance gene silencing or knocking-out expression frame, the sterile line fertility restoration expression frame, the expression frame capable of enabling maintainer line seeds to have a significant visible difference in color or shape in comparison with sterile line seeds, and the expression frame resulting in sterility of pollen containing the expression frame above. The biggest improvement of thehybridization system is that the reliability and stability of the whole hybridization system are greatly ensured by combining with a color/shape marking technology, a herbicidal selective killing technology and a transgenic pollen lethal technology, and the purity of hybrid seeds is ensured through multiple technologies, and the average purity is improved from 95% to 98%.

The present invention relates to crossbreeding, back crossing, etc. with temperature sensitive polCMS as female parent and selfing incompatible line capable of maintaining its incompatibility as male parent to shift the selfing incompatibility gene into the temperature sensitive polCMS and breeding cytoplasmic male sterility+ selfing incompatible line selectively from the offspring with high temperature sterility and low temperature fine powder selfing incompatibility; top crossing and back crossing of the line with available rape variety and screening to breed maintainer line and restoring line; maintaining the sterile and selfing incompatibility of the cytoplasmic male sterility+ selfing incompatible line with the maintainer line and restoring the bearing capacity of the cytoplasmic male sterility+ selfing incompatible line with the restoring line to form the matched three lines. The present invention has low breeding cost and risk, high hybrid purity and high heterosis.

The present invention discloses a breeding method for pod dehiscence-resistant cytoplasmic sterile line and pod dehiscence-resistant cytoplasmic maintainer line of brassica napus, and belongs to a cultivation method for a new crop variety. With the method provided by the present invention, the detection method for pod dehiscence resistance of the rape is improved; the pod dehiscence-resistant germplasm resource is screened; the screened pod dehiscence-resistant line H155 and the cytoplasmic sterile line 077A are subjected to hybridization, and continuous 8 backcrosses to breed the cytoplasmic sterile line H155A, wherein the sterility rate of the H155A is 100%. According to the present invention, the H155A has characteristics of micro pollen at the low temperature, complete sterility at the high temperature, good agronomic characteristics, cold resistance, viral disease resistance and sclerotium disease resistance. The erucic acid content and the glucosinolate content in the H155A is consistent with the erucic acid content and the glucosinolate content in the cytoplasmic maintainer line of the H155A. The pod dehiscence-resistant cytoplasmic sterile line and the pod dehiscence-resistant cytoplasmic maintainer line of the brassica napus establish the foundation for breeding the domestic pod dehiscence-resistant cytoplasmic sterile line hybrids.

The invention discloses a method for quickly cultivating a novel cytoplasm male sterility line of rice. The method comprises the following steps of: using dongxiang wide rice as a female parent and a variety or a strain which is good in column exposure, excellent in comprehensive character and good in combining ability as a male parent to hybridize to obtain F1; and then, performing backcrossing by using the varieties and strains as the backcrossing male parents; and from second backcrossing generate, selecting a single plant with pollen sterility phenotype similar to that of the backcrossing male parent to perform backcrossing with the backcrossing male parent, and continuously backcrossing for 5-6 times to obtain the male sterility line with dongxiang wide rice as an aborted cytoplasm source. The backcrossing male parent variety or strain is a maintainer line of the male sterility line. The novel cytoplasm male sterility line of rice can be cultivated in an accelerated manner by the technical method provided by the method disclosed by the invention, and the method is good in repeatability and reliable in result.

The invention discloses a breeding method of a yellow bud marker photosensitive male sterile line. The breeding method provided by the invention comprises the following step: performing autocopuation on the yellow bud marker photosensitive male sterile line under the condition of being within 11 hours and 12 hours and 20 minutes so as to get seeds of the yellow bud marker photosensitive male sterile line. The yellow bud marker photosensitive male sterile line is the sterile line, is also a maintainer line of the sterile line, and further has the characteristics of a wide range of restoration sources, free assembly, simple seed production procedure and low seed production cost. The yellow bud marker photosensitive male sterile line comprises a yellow bud marker, hybrid seeds can be effectively removed by screening markers during the seedling stage and photosensitive sterile strains can be further determined. The yellow bud marker photosensitive male sterile line can provide seed resources for breeding of the new photosensitive sterile lines, greatly reduce the cost of artificial emasculation, assemble a large number of hybrid combinations, breed the hybrid seeds and accelerate the breeding process.

The invention discloses nude hybrid rice and a breeding method thereof, and belongs to the technical field of the utilization of rice heterosis. The nude hybrid rice can be obtained through mutagenesis according to the characteristics that the nude character of rice is controlled by a pair of recessive genes. A maintainer line (B line), a two-line sterile line (S line) and a restorer line (R line) of hybrid rice which has excellent performance on the production is subjected to direct irradiation mutagenesis to obtain nude gene mutants corresponding to the maintainer line, the two-line sterile line and the restorer line of the hybrid rice. A nude maintainer line 'nude B' is hybridized and backcrossed with a corresponding A line to obtain a nude sterile line 'nude A' with the nude character. The 'nude A' can be assembled with 'nude R' to obtain nude three-line hybrid rice; and 'nude S' can be assembled with the 'nude R' to obtain nude two-line hybrid rice. The nude hybrid rice is high in photosynthetic efficiency, and is easy to manually harvest, mechanically harvest and thresh; and the characteristics of increasing the storage capacity of the rice, reducing working dust and the like ensure that the nude hybrid rice has relatively strong market competitiveness.

The invention discloses a method for breeding a male sterile line by use of the sterile cytoplasm of hibiscus cannabinus KN250, which specifically comprises the following steps: performing saturation backcross by taking a breeding material containing the sterile cytoplasm of hibiscus cannabinus KN250 as a female parent and the cultivated variety of hibiscus cannabinus as a male parent, and selecting a uniform wild abortive type cytoplasmic male sterile line similar to the male parent in character and showing male sterility, wherein the male parent for backcross is a corresponding male sterility maintainer line. The method disclosed by the invention has the beneficial effects that the hybrid matched with the KN250 cytoplasmic male sterile line and the hybrid matched with the UG93 or H040 cytoplasmic male sterile line are popularized and utilized at the same time, and the occurrence risk of certain disease can be greatly reduced.

A process for selectively culturing the cytoplasmic male sterile line CMS6-150 of covering mustard includes such steps as continuous backcrossings between the natural mustard-type rape's cytoplasmic male sterile line 6-102A as female parent and the maintainer-line covering mustard 6-150 as male parent while molecular marker aided selecting to obtain the cytoplasmic male sterile line and maintainer line of covering mustard, and reproducing said cytoplasmic male sterile line.

The invention discloses a brassica oleracea seed production technology, an adopted seed production method comprises the steps that a lateral bud is cut from a knot position and cultured on a culture medium, and subculture is performed in a lab continuously to obtain an original mother plant; seed reproduction of a sterile line is performed; a sun bed is prepared; a seeding tray is prepared; provisonal planting of the mother plant is performed; maintainer line seedling growing is performed; field planting is performed; pollination is performed; ventilation harvesting is performed; in the podding mid-term, maintainer line rows are removed; the row space is increased to facilitate ventilation and light transmitting, 70% of pods are cut when yellowing and dried for threshing after ripening; male parent seed reproduction is performed; the bud selection standard is strictly required, the bud stripping and pollination operation is standardized, and pollination marks are made; coeno-species seed reproduction is performed. According to the seed production technology, the yield can reach 65-75 kg/mu, the germination rate and the germination potential can reach up to 85% or above, the seed yield is high, and a foundation is laid for brassica oleracea stable production; after planting, the species has remarkable advantages over the other species on whether the disease resistance, the cold resistance, the physical shape or the yield, and the brassica oleracea is convenient to popularize, promote and plant.

The invention discloses a breeding method of a three-line sterile line of indica type rice with high combining ability. The method comprises the following steps of firstly, preparing 25 homogeneous restorer lines with different cytoplasm types, then hybridizing the 25 homogeneous restorer lines as a female parent with a newly bred maintainer line, measuring the general combining ability of the maintainer line and the special combining ability of cytoplasm of the maintainer line and the sterile line, determining a donor sterile line providing sterile cytoplasm according to the measurement result of the combining ability, performing transfer breeding of the sterile line by taking the sterile line as a transfer breeding female parent and the maintainer line as a male parent, and after the breeding of a new sterile line, performing combing ability verification by virtue of restorer lines with different sources. According to the breeding method disclosed by the invention, the homogeneous restorer lines prepared in one step can be used for a long time, and can be used as early as possible to determine the combining ability of the maintainer line, and the optimum sterile cytoplasm can be selected to perform transfer breeding of the sterile line so as to ensure that the newly bred sterile line has high combining ability, so that the possibility of idle work and shooting at random of the transfer breeding of the sterile line are reduced. Corresponding measures can be taken for the lines with high combining ability to accelerate the breeding process and improve the breeding efficiency.