98 results about "Backcrossing" patented technology

A method for selectively culturing the new variety of wheat includes such steps as respective crossing between the wheat 'Aibai' as female parent and 15-20 excellent varieties as male parent to obtain F1 generation, respective backcrossing between the dwarf sterile plants as female parent and the said male parent to obtain the backcrossed seeds F1, mixing them together, free pollination between fertile plants and sterile plants to obtain the seeds, mixing, free pollination between dwarf sterile plants and non-dwarf fertile plants to obtain seeds A, controlled pollination between the high-quality dwarf sterile plants as female parent and the specific plants as male one to obtain seeds B, proportionally mixing seed A with B, repeating said steps to obtain improved group, and culturing the excellent fertile plants by genealogical method.

The invention relates to a breeding method of hybrid cabbage, in particular to a simplified breeding method for preparing hybrid cabbage by using cytoplasmic male sterile lines, which belongs to the technical field of plant culture. The invention directly adopts the cabbage varieties of Zhonggan No.22 and Hugan No.2 prepared by cytoplasmic male sterile lines sold in the market as maternal plants, and adopts cabbage inbred lines (with the same ball shape as the maternal plants, the same type of leaf ball mature performance as the maternal plants and stable botanical characters) as pollen suppliers for preparing good coenospecies. The invention has the advantages that when the advantages of using the cytoplasmic male sterile lines for preparing the cabbage coenospecies are maintained, good cabbage inbred lines do not need to be used as recurrent parents for fifth to seventh generation backcrossing and transformation with male sterilant materials, the labor is saved, the cost is saved, the breeding time is greatly shortened, and in addition, the hybrid vigour parts of the original varieties can be maintained into the new hybrid varieties.

The invention discloses a continuous recessive character backcrossing technology which is characterized by taking a material which contains no target recessive genes and is ready to be improved as a recurrent parent, and taking a material which contains the target recessive genes as a donor parent; taking the recurrent parent as the parent to be hybridized with the donor parent and harvesting hybrid seeds of the first generation F1 and recurrent parent seeds; and planting the hybrid seeds of the first generation F1 and the recurrent parent seeds till the planting of a group containing backcross hybrid seeds of the second of n generations BCn F2 and till the typing into a target recessive gene isogenic line of the recurrent parent. The invention also discloses a method for creating a purpleleaf marker isogenic line by applying the continuous recessive character backcrossing technology and also a method for transferring new purple leaf marker nucleus sterile line by taking the purple leaf marker isogenic line as an intermediate material. In the methods, a selfing process is combined with a next backcrossing process for the treatment in one season, therefore, the time efficiency is improved by about 50 percent, a small number of dominant-gene-controlled unsuitable characters of existing fine-quality parents can be fast improved, and useful recessive characters can be fast imported to the present high-level heredity background.

The invention belongs to the field of cotton genetic breeding, and relates to a precocity short-fruit-spur upland cotton new-germplasm breeding method. The method includes the steps that hybridization and backcrossing are carried out with simian 2 as a female parent and gossypium klotzschianum as a male parent to obtain F1 plants and BC1F1 plants capable of normally growing; multi-generation backcrossing is carried out through simian 2 continuously, then backcross descendants are separated through selfing, zero type fruit spur families and first type fruit spur families good in prematurity and yielding ability are selected in separated groups, and precocity cotton new germplasm with the growth period of about 105 days, the zero-type or first-type fruit spurs, the compact plant type, concentrated boll opening, the good boll-setting performance, the high ginning outturn and the good fiber quality is bred through continuous multi-generation selfing and field orientation optimization in cooperation with an indoor plant laboratory test. The precocity source comes from the gossypium klotzschianum, and the hereditary basis of precocity breeding is expanded; precocity genes in the gossypium klotzschianum can be used by breeding and are of great significance in science and production.

The invention discloses a QTL-seq based method for mining a cold-tolerant gene of Dongxiang wild rice. According to the method, cultivated rice 'Xieqing Zao B' is taken as a receptor parent, namely, a female parent; the Dongxiang wild rice is taken as a male parent; a recombinant inbred line BC1F10 obtained by distant hybridization and continuous backcrossing is subjected to cold tolerance identification; obtained 20 cold-tolerant strains form a cold-tolerant gene pool; and 20 cold-sensitive strains form a cold-sensitive gene pool. The two gene pools are subjected to high-throughput sequencing and QTL-seq analysis to obtain differential SNP makers and cold-tolerant character related QTL loci of the two gene pools; candidate cold-tolerant genes are subjected to fluorescent quantitative PCR detection; and finally the Dongxiang wild rice is detected to contain five cold-tolerant related genes by identification. According to the method, the cold-tolerant genes of the Dongxiang wild rice can be quickly and accurately detected and mined, a powerful evidence can be provided for comprehensively illuminating a genetic mechanism of cold tolerance character of the Dongxiang wild rice, a foundation is laid for cloning and functional analysis of the cold-tolerant genes of the Dongxiang wild rice, and the breeding practice of the cold-tolerant genes of the Dongxiang wild rice is facilitated.

The invention relates to a method for hybrid rice variety breeding and safe and high-efficiency seed production by use of sensitivity character of a weed killer and color character of a specific rice husk. The method is characterized in that marker characters (weed killer sensitive lethal and specific rice husk color) are utilized and genetic improvement methods such as hybridization, backcrossing, self-crossing and the like are comprehensively utilized, double differences of the rice husk color and the weed killer sensitivity are created between parents of hybrid rice, male parent and female parent of the hybrid rice, containing different marker characters and suitable for mechanized seed production, are cultivated, the weed killer is sprayed for killing the male parent and the sorting technology of a seed color sorter is adopted according to the different marker characters after mechanical mixed sowing (transplanting), so that under the condition that the purity of the hybrid seed is guaranteed, the mechanized hybrid rice seed production is realized, the seed production efficiency is improved, and safe and high-efficiency production of the hybrid rice seed is achieved.

A method for selectively culturing the pure line of tomato parent resisting against southern Meloidogyne by using the female parent of a hybrid tomato as the object to be improved and the hybrid tomato resisting against Meloidogyne as antigen includes such steps as molecular marker and resistance discriminations to antigen B, artificial pollination between A as female parent and B as male parent, continuous backcrossing by 5-6 generations by using C as recurrent parent and selfing by 2 generations.

The invention relates to the field of breeding and specifically relates to a multi-ecological-area recurrent mixed selection - mixed planting cotton breeding method, which comprises the following steps of: 1) preparing cotton hybridized combinations; 2) reproducing generation F1 hybrid seeds in the south for increasing the generation; 3) respectively sowing generation F2 seeds in cotton regions of the Yellow River Valley, the Yangtze River Valley and the northwest inland; 4) selecting generation F3 seeds of excellent individual plants and reproducing in the south for increasing the generation; 5) respectively sowing generation F4 seeds in the cotton regions of the Yellow River Valley, the Yangtze River Valley and the northwest inland; and 6) selecting generation F6 seeds of excellent individual plants, sowing the seeds in hybrid seed production fields and selecting excellent individual plants according to breeding purposes. The method provided by the invention complements a modified backcrossing and recurrent selection method and improves the yield, the quality and the insect resistance. The method has the advantages of high breeding efficiency, diversified varieties, high adaptability, high purity and good consistency, and lays a foundation for breeding better varieties in future.

The invention provides a molecule marker of brown planthopper resistant gene. According to the present invention, through the hybridization and backcrossing of a resistant parent RBPH54 and a susceptible variety TN1, progenies are obtained. Through respective resistance identifications and molecule genetic linkage analysis of the progeny strains, a brown planthopper resistant major recessive genebph20(t) is obtained. Two nearest molecule markers linked beside bph20(t) are BYL7 and BYL8, which are self-developed SSR markers. The distances from the markers to bph20(t) are 1.3cM and 1.2 cM. With molecule markers linked to the brown planthopper resistant gene, the detection can be carried out to detect whether the pest resistant variety RBPH54 or the derivative varieties (systems) of RBPH54 contain the pest resistant genetic locus. Therefore, the selection of brown planthopper resistant paddy varieties or paddy qualities can be substantially improved in efficiency.

A breeding method of improving powdery mildew resistance of weak gluten wheat Ningmai-9 by utilization of backcrossing is disclosed. According to the method, the Ningmai-9 with high excellent-character combining ability and high genetic transmitting ability is adopted as a recurrent parent, Yangmai-10 carrying a powdery mildew resistant Pm4a gene is adopted as a nonrecurrent parent, and through hybridization and limited backcrossing assisted by generation selection of powdery mildew resistance, agronomic characters and quality characters, Ning0556, Ning0564, Ning0569, and the like which are new wheat strains with good yielding ability and high powdery mildew resistance are bred. Through identification of yielding ability, adaptability, disease resistance, quality, and other characters, the Ning0569 is the most prominent. The Ning0569 is subjected to wheat pilot experiments of the national middle and lower Yangtze river winter wheat group and the Jiangsu province Huainan group respectively, ranks the first of the two groups with respect to yield, and is named as Ningmai-18 by Jiangsu province and the national approval. The Ningmai-18 has advantages of high resistance to powdery mildew and high acre yield, and is suitable for popularization and application.

The invention discloses a novel selective breeding method for a male sterile line of Sichuan preserved pickle cytoplasm brasica napus. According to the method, a sterile source of the Sichuan preserved pickle is transferred into the brasica napus through distant hybridization and continuous backcrossing between different inter-species in brassica, so as to obtain Sichuan preserved pickle cytoplasm brasica napus CMS '93-12A'. The Sichuan preserved pickle cytoplasm brasica napus CMS '93-12A' is improved by hybridization and continuous backcrossing, so as to obtain a novel, stable, and double low sterile line material 'Jia rapeseed 58A'; the identification of the restoring and maintaining relationship of the Sichuan preserved pickle cytoplasm CMS shows that the restoring and maintaining relationship of the 'Jia rapeseed 58A' is different from the restoring and maintaining relationships of Pol CMS and Shaan 2A CMS, and meanwhile, one part of restorer material is screened out, and is improved to obtain five parts of double haploid restorer materials; the PCR identification with a specific primer shows that sterile sources of the '93-12A', the 'Jia rapeseed 58A' and mustard CMS are the same; an identification result of hybridization mating group yield of the 'Jia rapeseed 58A' and different restorer materials shows that a part of the combinations have a good yield-increasing potential, and the 'Jia rapeseed 58A' has a good combining ability. The method provided by the invention can be applied to the selective breeding of rapeseed varieties and the planting of the rapeseed in production.

The invention discloses a method for improving a brassica napus type temporary maintainer line of nuclear recessive epistatic interaction sterility in rapes. The method comprises the following steps: selecting a homozygous two-type line strain B to cap and self-cross, selecting an individual plant from a sterile segregation population to hybridize with a to-be-improved temporary maintainer line; then, taking the sterile segregation plant as a recurrent parent to continuously backcross and self-cross, and test cross to breed a new temporary maintainer line after the backcrossing; hybridizing the temporary maintainer line with the homozygous two type line to obtain a complete sterile line, wherein sterile plants account for 100% of the complete sterile line. The bred temporary maintainer line is stable in heredity, very close to the homozygous two type line genetic background, the obtained complete sterile line is easily compounded with a restoring line to prepare strong superiority hybridized combination, and a novel excellent hybrid rape variety is obtained by screening. The invention provides a novel technical method for widely applying a nuclear recessive epistatic interaction sterility pollination control system with a development prospect to the production practice.

The invention belongs to the field of genetics and discloses a breeding method for new varieties of drought-resistant wheat. Gaocheng 9411 serves as the female parent, ATHLET serves as the male parent, continuous selection is conducted through plant-to-row for generations, and the drought-resistant wheat strains JM205 and JM208 high in waxiness are created; Jimai 22 serves for backcrossing and conducts backcrossing with the drought-resistant wheat strains JM205 and JM208 high in waxiness respectively, six generations of BC6F-5 and BC6F1-8 strains are backcrossed for expanding propagation, waxiness of flag leaves is tested, testing and calculation of physiological indexes about drought resistance are conducted, and the wheat strains J-JM205 and J-JM208 which are high in waxiness, resistant to drought and high in yield are created. According to the breeding method for the new varieties of the drought-resistant wheat, conventional visual inspection conducted at the early stage of backcrossing and index testing conducted at the later stage are adopted, breeding cost is saved at the early stage, and accurate selection is conducted at the later stage.

The invention relates to the field of molecular biology, in particular to a method for detecting a klenow fragment repetition position in a saccharomyces cerevisiae chromosome.By combining genomic sequencing with endoreduplication backcrossing, the position of repeated klenow fragment genome DNA in the chromosome is confirmed quickly.Through the endoreduplication backcrossing method, the position of the repeated klenow fragment genome DNA in the saccharomyces cerevisiae chromosome can be detected efficiently and quickly and repaired, repeated klenow fragment genome DNA is deleted, there is no need to precisely confirm the position of a klenow fragment repetition site or conduct cumbersome experimental data analysis on saccharomyces cerevisiae to be detected, operation is easy, and the experimental period is short.

The invention discloses a method for breeding a normal corn inbred line by using a hard grain type waxy corn inbred line. According to the method, a corn inbred line is bred with a method of one-time hybridization of the early-maturing hard grain type waxy corn inbred line and repeated backcrossing of normal corn, so that rapid breeding of a new normal corn species with excellent quality, high unit weight and early maturity is realized. With the adoption of the method, the quality of the normal corn inbred line is improved, the hereditary basis of normal corn is widened, types of the normal corn are enriched, the corn growth period is shortened, and the method has the important social significance and economic value.

The invention discloses a breeding method for limited fruit branch type short-season cotton suitable for being planted in Yangtze River valley cotton areas, and relates to the field of crop breeding. The method has the advantages that the integral analysis is performed on the basis of agricultural natural resource conditions of light, temperature, water, soil and the like in the Yangtze River valley cotton areas; short season cotton varieties (limited growth type early-maturing cotton germ plasm) in Yellow River valley cotton areas are used as male parents to be treated by breeding methods such as hybridization, backcrossing and system selection with cotton varieties (high-quality high-yield and high-adaptability cotton germ plasm) in the Yangtze River valley cotton areas; disease-resistant high-temperature-resistant high-yield and high-quality new limited fruit branch type short-season cotton varieties adapting to the mechanical high-density planting in the Yangtze River valley cotton areas are obtained.

The invention relates to methods for breeding the breeding materials of wheat and triticale. The method for breeding the wheat comprises the following steps: the new wheat idioplasm Lankao 52-54 is selected as a male parent, and wheat 0308 is selected as a female parent, and the male parent and the female parent are hybridized; and a filial generation and the male parent are carried out repeated backcrossing. The method for breeding the triticale comprises the following steps: No.8 triticale Lankao is selected as a male parent, the wheat 0308 is selected as a female parent, and the male parent and the female parent are hybridized; and a filial generation and the male parent are carried out repeated backcrossing. The wheat and the triticale which are bred by the invention have compatible hybridization with mediterranean agropyron and are easy to fruit; the excellent characteristics of the mediterranean agropyron can be inherited; and a plurality of new types and species of ice wheat and ice triticale can be bred.

Described herein are methods and materials useful for improving root growth and nutrient uptake in cereal grasses. In particular, present disclosure provides methods for increasing root growth and nutrient uptake in a cereal grass involving marker assisted selection and backcrossing. The present disclosure also provides recombinant DNA for the generation of transgenic plants, transgenic plant cells, and methods of producing the same. The present disclosure also provides materials and methods useful for improving the tolerance of a cereal grass to phosphorus-deficiency The present disclosure further provides methods for generating transgenic seed that can be used to produce a transgenic plant having increased root growth, nutrient uptake, and phosphorus-deficiency tolerance.

The invention belongs to the field of genetics, a molecular marker for rapid detection of high yield gene of Thinopyrum elongatum and its application are provided, by conventional hybridization, backcrossing and greenhouse and artificial climate chamber generation techniques, a near-isogenic line of high yield gene 7el2L of Thinopyrum elongatum was created. Combined with fluorescence in situ hybridization (FISH) and analysis of agronomic and yield-related traits, molecular markers closely linked to 7el2L were obtained, which could be used in wheat breeding for high yield and early molecular-assisted selection for yield-related traits, in order to improve breeding efficiency.

The invention belongs to the technical field of flower breeding, and particularly relates to a transformation method of a zinnia male sterile dual-use system. The invention takes a sterile plant of a zinnia male sterile dual-use system AH209AB as a parent plant, takes a zinnia inbred line as a father plant, and after cross breeding, inbreeding, continuous backcrossing, group pair hybridizing and restoring and maintaining test, an orangered single recessive zinnia male sterile dual-use system J16AB is finally transformed and obtained. The invention can transform a male sterility gene into an inbreeding line with excellent properties, obtains the zinnia male sterile dual-use system with consistent gardening properties, thorough abortion and stable fertility inheritance, enriches the breeding materials, and lays the foundation for the zinnia crossbreeding. During the transformation process, through the phenotype characteristics of floral organs, the invention can accurately analyze and judge the fertile plants and the sterile plants of the zinnia, and provides phenotype marks for zinnia fertility identification.

A method for increasing the high yield potential of hybridized rice includes hybridizing and continuous backcrossing to introduce the high-yield genes yld 1.1 and / or yld 2.1 of Malaysian wild rice O. rufipogon to the parent of hybridized rice, descriminating the plants carrying said high-yield gene by molecular marker aided selection, analysis, screening the dominant plants, breeding the strong and dominant recovery line, and pairing the combination of cross rice.

The invention belongs to the field of transgenic plant detection, and particularly relates to a transformant specific detection primer for transgenic insect-resistant and herbicide-resistant corn CM8101 and application thereof in backcrossing. A PCR method of the primer can serve as an effective means for identifying CM8101 transformants and is applied to backcrossing of CM8101 transformant insect-resistant genes. The method has the advantages of rapidity, high sensitivity, good specificity and low experimental condition requirement, thereby having very high practical value. The method provides a supervision means for further application of CM8101.

The invention relates to a pink male sterile line transformation and a cultivation method for the breed and coenospecies, belonging to the technical field of plant cultivation. The method uses the existing pink male sterile line as a female parent and at least two pink self compatible lines as male parents, and biparental cross is carried out respectively with the pink male sterile line as the female parent; a single plant mostly close to the economic character of the male parent is selected from the later generations of the biparental cross as the female parent and then backcrossed with the original male parent; the backcrossing is repeated, and after at least five generations of backcrossing and transformation, the pink male transformation sterile line is obtained. The pink male transformation sterile line obtained by adopting the method of the invention has the advantages of stable male sterility, better combining ability, and the like; by means of multiple self-crosses and line-crosses, most combinations show the heterobeltiosis of disease resistance, growing luxuriance, flowering branch increasing, colored flower, and the like; the labor and cost can be obviously saved.

The invention provides a breeding method and application of a high-quality Chinese pumpkin variety without vines and seeds, and relates to the technical field of crop breeding. The breeding method comprises the following steps: with a vine-free oblate spheroidal Chinese pumpkin homozygous material A and a long-vine Chinese pumpkin homozygous material B with seed plate length of 2.5cm or more, plate width of 1.3cm or more, or single fruit seed number of 520 or more, and with a parent B as a recurrent parent, carrying out continuous optimal backcrossing and self-crossing to obtain BCn+1Fm+1; selecting a vine-free BCn+1Fm+1 single plant with seed plate length of 2.2-3 cm, plate width of 1.1-1.5 cm or single fruit seed number of 450 or more, and carrying out continuous self-crossing to obtaina homozygous inbred material C; and crossbreeding the homozygous inbred material C and a long-vine Chinese pumpkin homozygous material E to obtain the Chinese pumpkin without vines and seed. The breeding method provides a special variety of Chinese pumpkins for seeds, which combines the excellent traits of three parents, has no vines, has more than 350 seeds on each single fruit, excellent plate length, plate width and 100-grain weight, is conducive to close planting and can bring considerable economic benefits to enterprises and farmers.

The invention provides a method for cultivating very early-maturing rice and belongs to the field of crop genetics and breeding. The invention relates to the utilization of a main spike early-maturingrice material, for a characteristic that a main spike maturation period and a tiller spike maturation period of the main spike early-maturing rice material have a large difference, and a strategy forreducing the tiller number of the rice and shortening the maturation period of the whole plant is proposed, which means that the purpose of controlling the rice maturation period is achieved by controlling the tiller number of rice. A less tiller early-maturing rice material is created through a cross breeding technique or genome editing technique, then the less tiller early-maturing rice material and excellent rice varieties are hybridized, followed by continuous backcrossing, a less tiller early-maturing rice individual is selected, a less tiller early-maturing excellent rice variety with agrowth period within 90 days is obtained, and the early-maturing rice variety is particularly suitable for direct seeding cultivation in rice production.

The invention discloses a mutant gene OsCOPT7 of rice copper low accumulation mutant lc1 and application of the gene. According to the mutant gene OsCOPT7 of rice copper low accumulation mutant lc1, abase mutation occurs in the coding region of a wild OsCOPT7 gene, namely, the 344th base downstream of ATG is mutated from G to A, and the 115th codon in the coding region is changed from GGG to GAG,so that the amino acid at position 115 in the protein sequence is mutated from glycine (Gly / G) to glutamic acid (Glu / E). the coding nucleotide sequence of the mutant gene OsCOPT7 is shown in SEQ ID No. 1, and the amino acid sequence is shown in SEQ ID No. 2. The copper content in rice grains of mutant lc1 is significantly lower than the copper content in rice grains of the wild type. The application of the mutant comprises one or more of transgene, hybridization, backcrossing or asexual reproduction; and the application of the mutant gene OsCOPT7 comprises enabling rice to contain the mutantgene OsCOPT7, or enabling rice to contain the amino acids encoded by the mutant gene OsCOPT7.

The invention relates to a method for screening mycosphaerella melonis-resistant IL 14 of cucumber-pickled cucumber introgression line. According to batch treatment of different concentrations, plants with good resistance are screened, and the method belongs to the field of biotechnology breeding. The method comprises the following steps: by taking wild species pickled cucumber as a female parent, cultivating cucumber 'Beijing jietou' as a male parent, and hybridizing to obtain hybrid F1; combining the hybrid F1 with a somaclone variation chromosome reduplication technology by virtue of embryo rescue, thereby obtaining a fertile inter-specific heterologous tetraploid new species which is named as Cucumis hytivus; and by taking the heterologous tetraploid new species Cucumis hytivus as a female parent, performing system backcrossing and self-cross selective breeding with the cultivated variety 'Beijing jietou', thereby obtaining 86 parts of cucumber inter-specific introgression line materials carried with wild species chromosome segments, and respectively numbering as IL1-IL86. The 86 parts of introgression line groups are screened by adopting the greenhouse seedling stage artificial inoculation identification technology, stable moderate resistance or more strains are obtained by virtue of inoculation treatment in spring and autumn, the inoculation treatment is performed by increasing the concentration of bacterium, and the mycosphaerella melonis resistance strain IL14 is screened.

The invention discloses a method for culturing new lines of superhigh vitamin C Kiwi berry, which relates to a method for culturing the new lines of Kiwi berry. The method comprises the following steps: 1) determining a first generation cross parent; 2) developing a first generation interspecific crossing; 3) performing male plant breeding of F1-generation high vitamin C; 4) developing interspecific backcrossing; 5) evaluating a F2-generation colony system; and 6) obtaining the new lines F2-EC of superhigh vitamin C. Compared with the prior art, the method has the following advantages and active effects that 1) breeding obstacle of dioecism plant can be overcome; 2) he Kiwi berry vitamin C content is greatly increased; 3) interspecific crossing and interspecific backcrossing are employed, the Kiwi berry new lines with excellent quality and superhigh vitamin C content can be cultured; and 4) the method is suitable for performing directive breeding on high-VC content Kiwi berry lines.