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Method for breeding parent pure line of tomato with function of anti-Meloidogyne incognita Chitwood

A root-knot nematode and tomato-resistant technology, applied in botany equipment and methods, biochemical equipment and methods, microbial measurement/testing, etc., can solve problems such as difficult to distinguish resistance genes, complicated procedures, and inaccurate results , to achieve the effect of solving the cumbersome identification procedures and high coordination

Inactive Publication Date: 2007-01-31
NORTHWEST A & F UNIV +1
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Problems solved by technology

The breeding of resistant varieties depends on the discovery and utilization of resistant resources. The traditional artificial inoculation identification method not only has complicated procedures and inaccurate results, but also makes it difficult to distinguish between homozygous and heterozygous resistance genes, resulting in homozygous resistance genes. The speed is slow, which seriously restricts the breeding of tomato root-knot nematode-resistant parent lines and new varieties

Method used

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  • Method for breeding parent pure line of tomato with function of anti-Meloidogyne incognita Chitwood
  • Method for breeding parent pure line of tomato with function of anti-Meloidogyne incognita Chitwood
  • Method for breeding parent pure line of tomato with function of anti-Meloidogyne incognita Chitwood

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Embodiment

[0036] (1) The tomato hybrid "Jinpeng No. 1" with excellent comprehensive traits but not resistant to root-knot nematode in my country was used as the female parent (variety A, provided by Xi'an Jinpeng Seedling Co., Ltd.), and tomato hybrids imported from abroad containing resistance The hybrid Kelly (B variety, provided by Shandong Shouguang Syngenta Seed Industry Co., Ltd.) with the root-knot nematode gene Mi was used as the male parent for hybridization.

[0037] (2) Identification of molecular markers at seedling stage and identification of disease nursery resistance at adult plant stage for antigen B. The specific method is: first sow the source of resistance B, and when the seedlings grow to 2 to 3 true leaves, use the micro method to extract the total DNA of a single leaf (Gong Zhenhui et al., Micro DNA extraction method for identification of transgenic plants by PCR. Northwest Agriculture University Journal, 1997, 25(1): 45-48). Forward and reverse primers were design...

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Abstract

A method for selectively culturing the pure line of tomato parent resisting against southern Meloidogyne by using the female parent of a hybrid tomato as the object to be improved and the hybrid tomato resisting against Meloidogyne as antigen includes such steps as molecular marker and resistance discriminations to antigen B, artificial pollination between A as female parent and B as male parent, continuous backcrossing by 5-6 generations by using C as recurrent parent and selfing by 2 generations.

Description

technical field [0001] The invention belongs to the field of tomato breeding, and relates to a tomato breeding method, in particular to a tomato parent pure line breeding method resistant to root-knot nematode incognita. The method combines molecular marker-assisted selection and traditional selective breeding methods to screen The resulting pure parental lines can be directly used in the mating of root-knot nematode-resistant tomato hybrids. Background technique [0002] Root-knot nematode (Meloidogyne spp.) is one of the most serious soil-borne diseases of tomato (Lycoperisicon esculentum), and its main species is southern root-knot nematode (Meloidogyneincognita). With the expansion of tomato protected cultivation area and the increase of multiple cropping index, the harm of root-knot nematode is becoming more and more serious. The yield loss of diseased fields is generally 30% to 50%, and in severe cases it can reach more than 70%. For the chemic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04A01H5/00C12Q1/00
Inventor 巩振辉逯明辉黄炜李大伟马维李晓东郑丽粉王建人蔡义勇
Owner NORTHWEST A & F UNIV
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