3377 results about "Molecular marker" patented technology

The invention relates to the technical field of plant breeding, in particular to a molecular marker related to oil content and protein content of soybean and application of the molecular marker. The molecular marker is constructed on the basis of SNP sites; based on a genome version number Wm82. A2. V1, the SNP site is located at the 8605814th site of the No.8 chromosome of the soybean, and the polymorphism is A / G. The application comprises the following steps: (1) predicting or detecting the oil content or protein content of soybeans; (2) identifying or cultivating soybean varieties with high oil content or protein content; (3) soybean molecular marker assisted breeding; (4) soybean variety improvement related to oil content or protein content; and (5) soybean germplasm resource improvement. The molecular marker related to the soybean oil content and the protein content is obtained through research and screening, and the molecular marker can be applied to cultivation of soybean varieties with high oil content and high protein content and has important value in the field of soybean breeding.

The invention belongs to the technical field of pepper planting, and discloses a pepper multi-branch gene CaBr1 linked KASP molecular marker as well as a primer, a kit and application thereof, the KASP molecular marker takes pepper S8 (zhangshuang) as a reference genome, and the single nucleotide polymorphism at the 103175415th basic group on the No.8 chromosome is subjected to basic group G-to-A replacement. A chromosome region closely linked with multiple branches of the pepper is obtained by using a BSA (Bovine Serum Albumin) population positioning method, a molecular marker is developed in a candidate region to determine a candidate gene CaBr1, a KASP molecular marker is screened according to base mutation of the candidate gene, and the marker is used for carrying out genotype identification on 90 randomly sampled single plants of an F2 population, and the coincidence rate reaches 100%. The result not only contributes to pepper branch phenotype identification and assistant breeding, but also lays a foundation for map-based cloning of the branch gene and analysis of a molecular mechanism of pepper plant type regulation.

The application provides a wheat flour wet gluten content main effect site and SNP marker and application, and belongs to the wheat breeding technical field, and mainly provides SNP molecular markers Marker45889, Marker45896, Marker45348, Marker45403, Marker45397 and Marker45431, and the SNP site provided by the application has important significance for detecting and breeding wheat flour high FWG varieties, lines and breeding materials, and molecular markers can be developed according to the SNP site, so that the wheat quality breeding efficiency is accelerated.

The invention belongs to the technical field of genetic breeding, and particularly relates to application of a molecular marker for breast circumference character breeding of grassland short-tailed sheep, the molecular marker is at least one of the following (1)-(3): (1) a nucleotide sequence as shown in SEQ ID NO.1, nucleotide at the 101bp site is A or G; (2) a nucleotide sequence as shown in SEQ ID NO.3, wherein the nucleotide at the 101bp site is C or A; (3) a nucleotide sequence as shown in SEQ ID NO.5, wherein the nucleotide at the 101bp site is T or C; the application refers to any one of the following steps: identifying the chest circumference of the grassland short-tailed sheep; the chest circumference of the offspring of the grassland short-tailed sheep is improved. By detecting the genotype of the molecular marker and selecting a dominant genotype individual as a parent, the average chest circumference of offspring can be effectively improved, and a reliable tool is provided for realizing molecular assisted breeding and precise genetic improvement of the chest circumference character of the grassland short-tailed sheep.

The invention discloses a biomarker for evaluating litchi quality and a detection method, and relates to the technical field of biology. The marker is an SNP (Single Nucleotide Polymorphism) molecular marker, and the nucleotide sequence of the marker is as shown in SEQ ID NO.1-3. The molecular marker disclosed by the invention can be used for evaluating the quality of litchis, particularly evaluating the fructose content. Meanwhile, when the gene is applied to molecular marker assisted selection (MAS) and genome selection (GS), the genetic improvement progress of the litchi variety can be accelerated.

A molecular marker closely linked to a pepper fruit shape gene. Taking a basic version of Zhangshugang pepper as a reference gene, a single nucleotide polymorphism is present at base 162028839 of the pepper Chr02 chromosome, where a substitution from base C to A occurs. A primer and kit for identifying the molecular marker closely linked to the pepper fruit shape gene, and use thereof in identifying the pepper fruit shape or in molecular-assisted breeding. Early use of the molecular marker, the primer, and the kit in the early stage can quickly screen satisfactory plants, thereby effectively reducing the planting scale, reducing the workload of later identification, and improving the selection efficiency and accuracy. The molecular marker, the primer, and the kit are of great significance in the practice of pepper fruit shape breeding and the research on fruit shape changes and regulation mechanisms.

The invention relates to the technical field of molecular markers, in particular to an Indel molecular marker related to yellow leaves of a melon seedling growing point and application of the Indel molecular marker. The invention explores a key candidate gene MELO3C016136 for controlling the leaf color of the growth point of the muskmelon seedling, and develops an Indel molecular marker ygp2 for identifying the leaf color change of the growth point of the muskmelon seedling based on the key candidate gene MELO3C016136. The molecular marker ygp2 can be used for identifying the leaf color change character of the growth point of the muskmelon seedling, only one-time PCR amplification and polyacrylamide gel electrophoresis need to be carried out on the DNA of the plant to be detected, the identification result is reliable, the genotype of the target plant can be simply and conveniently identified, and the genotype identification result is completely consistent with the leaf color phenotype of the growth point of the seedling; important technical support is provided for creating new muskmelon germplasm with yellow seedling growing point leaves.

The invention discloses a positioning method of trachinotus ovatus growth trait related QTL (quantitative trait loci) based on 2b-RAD technology and application, the method uses 2b-RAD technology to perform sequencing on 300 trachinotus ovatus full-sib F1 generation and male and female parent individuals and develop SNP (single nucleotide polymorphism) markers, constructs a trachinotus ovatus high-density genetic linkage map, performs linkage positioning analysis in combination with 8 growth phenotype data, and finds that the trachinotus ovatus growth trait related QTL is found. 85 stable QTLs associated with the growth traits are screened out, and the stable QTLs contain 763 SNP sites. The invention also discloses an application of the method in a genetic map of trachinotus ovatus growth character positioning or molecular marker-assisted breeding of trachinotus ovatus, and the SNP molecular markers of trachinotus ovatus can be applied in genetic map construction and growth character positioning. Particularly, the method has a good application prospect in trachinotus ovatus growth trait molecular marker assisted breeding.

The invention belongs to the technical field of molecular markers, and discloses a molecular marker located in No.18 chromosome and related to the salt tolerance of soybean seeds in the germination period and application of the molecular marker. According to the present invention, the salt tolerance index of the soybean seed germination stage is determined, the stable and reliable molecular marker GmChr1856968562 closely linked with the salt tolerance ability of the seed germination stage is obtained through GWAS correlation analysis, the molecular marker is located at the 56968562 site on the No.18 chromosome of the soybean reference genome Glycine max Wm82. A2. V1, and the marker for KASP detection is developed based on the site, the invention also designs a primer group for amplifying the KASP marker. The invention further provides a method for rapidly identifying the salt tolerance of the soybean seeds in the germination period by using the primer group of the molecular marker, and the method is simple, convenient and rapid, accurate in identification result and good in popularization and application prospect.

The invention discloses a molecular marker primer group for identifying a macadamia nut variety Yunnan No.4, a kit and application. The molecular marker primer group is composed of three pairs of primers, and each pair of primers is composed of an upstream primer and a downstream primer. An upstream primer of the first pair of primers is as shown in SEQ ID NO: 1 in a sequence table, a downstream primer of the first pair of primers is as shown in SEQ ID NO: 2 in the sequence table, an upstream primer of the second pair of primers is as shown in SEQ ID NO: 3 in the sequence table, a downstream primer of the second pair of primers is as shown in SEQ ID NO: 4 in the sequence table, an upstream primer of the third pair of primers is as shown in SEQ ID NO: 5 in the sequence table, and a downstream primer of the fourth pair of primers is as shown in SEQ ID NO: 6 in the sequence table. The downstream primer of the third pair of primers is shown as SEQ ID NO: 6 in the sequence table. The molecular marker primer group can accurately identify the macadimia nut variety Yunnan No.4.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for identifying drought tolerance of wheat, haplotypes, primers and application. Belongs to the technical field of biological breeding. Research finds that three SNP loci exist on a wheat ARF20-B gene, a wheat drought-enduring haplotype ARF20-BHapI is developed based on the SNP loci, the first locus is located at the 3616bp position of the ARF20-B gene, and the A / G nucleotide polymorphism is A; the second site is located at the 5623bp position of the ARF20-B gene, and G is in the G / A nucleotide polymorphism; the third site is located at the 9795bp position of the ARF20-B gene, and G is in the G / A nucleotide polymorphism. The wheat carrying the ARF20-BHapI haplotype has the advantage that the drought tolerance of the wheat carrying the ARF20-BHapI haplotype is higher. The invention further provides a KASP primer group for detecting the ARF20-BHapI haplotype, and successful detection of the drought tolerance of the wheat is achieved.

The invention belongs to the field of plant seed biology and rice breeding, and discloses an application of an OsBTB05 gene or an OsBTB05 protein in regulation and control of storage resistance of rice seeds and a related breeding method. In particular discloses application of an OsBTB05 gene or an OsBTB05 protein in regulation and control of the storability of rice seeds, the nucleotide sequence of the OsBTB05 gene is shown as SEQ ID NO: 1 or SEQ ID NO: 2, and the amino acid sequence of the OsBTB05 protein is shown as SEQ ID NO: 3. The invention also discloses a molecular marker-assisted selection method for breeding the high-storage-resistance rice variety and a method for reducing the storage resistance of the rice seeds. The invention proves that the OsBTB05 gene is a key factor for regulating and controlling the storability of the rice seeds, and the expression level of the OsBTB05 gene is in significant positive correlation with the storability of the seeds. A clear molecular target and a brand-new gene resource are provided for improving the seed storage resistance from a genetic source.

The invention relates to the technical field of microbiological detection and molecular biology, in particular to a specific detection primer for bifidobacterium longum subsp. Longum BBMN68 and application of the specific detection primer. The specific primer for detecting the bifidobacterium longum subsp.longum BBMN68, provided by the invention, comprises an upstream primer and a downstream primer, and nucleotide sequences are shown as SEQ ID NO.7-8. The invention also provides a kit for detecting the bifidobacterium longum subsp.longum BBMN68. The specific DNA fragment provided by the invention is a specific molecular marker of BBMN68, and the specific primer is designed aiming at the fragment sequence, so that the specific amplification of the strain can be realized, no cross reaction is caused to other related strains, the detection sensitivity is higher, and the detection requirements of samples with different concentrations can be met; the method can be used for specific detection and accurate quantification of the BBMN68 strain in yoghourt and other products, and has good practical application value.

The invention relates to the technical field of molecular biology, and discloses 74 core molecular marker sets for identifying cold resistance of pennisetum alopecuroides, a primer group, a detection reagent, a kit, a gene chip and application thereof. According to the method, core germplasm resources of pennisetum alopecuroides are systematically collected, and the GWAS population is constructed by utilizing multi-environment (different altitudes) conditions, so that wide genetic and phenotypic variation is fully covered, and a representative material foundation is laid for cold-resistant genetic analysis. In the aspect of phenotype evaluation, a method for determining the number of reviving and root cutting seedlings after natural overwintering by combining single-root double-stem-node oblique cutting planting is innovatively provided, a set of scientific and stable cold resistance and reproducibility evaluation system capable of being operated in a standardized mode is established, and the reliability and efficiency of phenotype identification are remarkably improved. The detection is not influenced by human and climate environments; the method realizes efficient and accurate early cold resistance identification of pennisetum alopecuroides, greatly improves the breeding screening efficiency and scale, and accelerates the breeding process.

The invention relates to the technical field of genetic breeding, in particular to application of a molecular marker related to daily gain traits of sheep before weaning. The molecular marker is at least one of the following (1)-(2): (1) a nucleotide sequence as shown in SEQ ID NO.1, wherein the nucleotide sequence at the 101bp site is T or C; (2) a nucleotide sequence as shown in SEQ ID NO.3, wherein the nucleotide sequence at the 101bp site is T or C. The application refers to any one of the following (1) and (2): (1) identifying the daily gain of the sheep before weaning; and (2) the daily gain of the offspring of the sheep before weaning is improved. The screened molecular marker influencing the daily gain character of the sheep before weaning can be used for identifying the daily gain character of the sheep before weaning, and the daily gain of offspring before weaning can be improved by selecting a CC genotype individual as a male parent or a female parent.

The invention discloses an indel molecular marker for identifying color and luster of citrus peel in a seedling stage and application of the indel molecular marker. The corresponding sites of the indel molecular marker of the citrus are positioned in chr7: 18, 398, 798-18, 399 and 289 intervals of a chromosome 7 of an SWO3.0 reference genome; the genome DNA of the citrus seedling stage plant is used as a template, the Indel molecular marker is amplified through PCR, the citrus seedling stage plant can be divided into a homozygous insertion type, a homozygous deletion type and a heterozygous type according to a PCR amplification result, and the three types are associated with the color of the citrus fruit for identifying the color of the citrus peel, so that the early screening of the seedling stage target traits of the citrus breeding population is realized, and the early screening of the seedling stage target traits of the citrus breeding population is realized. The breeding period is remarkably shortened, the breeding cost is reduced, and important technical support is provided for citrus fruit color improvement breeding.

The invention provides a closely linked molecular marker primer of a rice brown planthopper resistant major gene Bph56 as well as a marking method and application of the closely linked molecular marker primer. According to the invention, genotypes of F2 individual plants obtained by hybridizing a rice insect-resistant variety C127 (or) and KW (or) are combined with brown planthopper resistance levels of an F3 family for genetic linkage analysis, a resistance major gene Bph56 carried by the insect-resistant variety C127 is identified, and a region of the resistance major gene Bph56 is reduced to a 77kb fragment limited by markers 6M26.278 and 6M26.355 on two sides and is closely linked with a molecular marker 6M26.355. The molecular marker 6M26.355 disclosed by the invention can be used for effectively detecting whether an insect-resistant variety C127 and derived varieties (lines) of the insect-resistant variety C127 contain the major resistance gene locus or not, so that the selection efficiency of the brown planthopper-resistant character is greatly improved, and the brown planthopper-resistant rice variety (line) containing Bph56 is obtained.

The invention provides a beet genetic diversity analysis method based on whole genome re-sequencing. The method comprises the steps of PacBio HiFi library construction, computer sequencing, data information analysis, assembly quality evaluation and Hi-C auxiliary genome assembly. The invention further provides application, and the high-continuity beet reference genome is used for analyzing beet intraspecific genetic diversity and developing high-density molecular markers. According to the method, the high precision of HiFi, the super-long read length of Nanopore and Hi-C space interaction data are combined for the first time, the problems of repeated sequence and complex SV analysis are solved, and all-round breakthrough of genome continuity, annotation integrity, genetic diversity coverage and epigenetic integration is achieved.

The invention discloses a molecular marker closely linked with a cowpea salt tolerance major QTL (quantitative trait loci) and a primer sequence of the molecular marker. The name of the cowpea salt tolerance major QTL is SC300126, a KASP type molecular marker 300126 closely linked with the cowpea salt tolerance major QTL is located at the position of 43816166 bp on a chromosome 11, the marker can be used for amplifying a material to be detected, and whether a sample carries key allelic variation for controlling salt tolerance or not is judged according to the genotype of the marker. The molecular marker and the primer provided by the invention can be used for quickly screening the cowpea salt tolerance major QTL, and compared with a traditional phenotype identification method, the molecular marker and the primer have the advantages of high detection flux, accurate and reliable result and the like, and the typing accuracy can reach 99% or above. The technology can significantly improve the breeding efficiency of the salt-tolerant variety of cowpea and shorten the breeding period.

The invention relates to the technical field of plant genetic engineering, and discloses a haplotype identification marker of a rice flood-resistant gene ARF9 and application of the haplotype identification marker. The molecular marker is derived from an exon of an ARF9 gene, is located at the 22009082bp and 22010019bp of a No.4 chromosome of rice, and has a basic group of C. The SNP molecular marker is related to the waterflooding resistance of a male parent oryza longistaminate of perennial rice, and the waterflooding resistance of the male parent oryza longistaminate with a locus genotype of CC is obviously higher than that of rice with a locus genotype of non-AA type. Therefore, the invention provides the loop-mediated isothermal amplification primer group for rapidly identifying the ARF9 gene haploid molecular marker, and the loop-mediated isothermal amplification primer group is used for identifying the ARF9 gene haploid molecular marker so as to rapidly screen out waterflooding-resistant rice. The invention provides a group of primers, the flood-resistant rice can be quickly identified by adopting the primers to perform loop isothermal reaction, the detection cost is low, the detection speed is high, the flood-resistant germplasm can be quickly screened in the rice seedling stage by adopting the method provided by the invention, a time-consuming flooding test does not need to be performed in the later stage, and the breeding process is greatly accelerated.

The invention relates to the technical field of biology, in particular to an SNP molecular marker for identifying upland cotton fiber quality, a KASP primer group and application of the SNP molecular marker and the KASP primer group. The invention provides five SNP (Single Nucleotide Polymorphism) molecular markers XW1-XW5 which are obviously related to fiber quality, and accurate and stable genotyping can be realized through verification. Wherein the fiber length can be remarkably increased by excellent allelotypes corresponding to the markers XW1, XW2, XW4 and XW5; the excellent allelotype corresponding to the marker XW3 can significantly improve the micronaire value, provides a reliable tool for upland cotton fiber quality identification and molecular breeding, and has important application value.

The invention belongs to the technical field of genetic breeding, and particularly relates to a KASP primer group for detecting the typing of a rice flowering time regulation gene OsMYB8 and application of the KASP primer group. The KASP primer group comprises a first forward primer, a second forward primer and a reverse primer; the nucleotide sequence of the first forward primer is as shown in SEQ ID NO. 10; the nucleotide sequence of the second forward primer is as shown in SEQ ID NO. 11; the nucleotide sequence of the reverse primer is as shown in SEQ ID NO. 12. The primer group disclosed by the invention can be used for rapidly and accurately identifying the genotype of the rice OsMYB8 gene, and can be applied to molecular marker-assisted breeding of OsMYB8 gene plants.

The invention discloses a molecular marker remarkably related to the amylose content of wheat and application of the molecular marker, and belongs to the technical field of wheat breeding. Three mutation sites of wheat starch branching enzyme gene SBEIIa on 2A, 2B and 2D chromosomes and two mutation sites of SBEIIb on 2A and 2B chromosomes are identified, a KASP molecular marker system closely linked with SBEIIa and / or SBEIIb is developed based on the SNP sites, the detection process is simple and convenient, the cost is low, the throughput is high, and the application prospect is wide. According to the method, the high-amylose wheat material which contains the mutant genotype and can be stably inherited can be accurately and efficiently screened out, the breeding process of wheat variety quality improvement can be accelerated, and the method has important significance on creation of the high-amylose wheat material or molecular assisted breeding of the high-amylose wheat.

The invention discloses a male sterility gene ZmUCE4 and application thereof in creating a male sterility line of corn, and belongs to the field of plant biotechnology breeding. According to the invention, site-directed mutagenesis of the ZmUCE4 gene in wild type corn is carried out through a CRISPR / Cas9 gene editing technology, pollen development abnormality and abortion can be caused, complete male sterility is caused, and it is proved that the ZmUCE4 gene has a regulation and control function on corn male reproductive development. Through offspring screening, a sterile line without transgenic components can be obtained, a stable maize male sterile line is created, and the method has important significance on maize male fertility control and hybrid seed production. The invention also designs a functional molecular marker aiming at the obtained uce4 male sterility mutant gene, and has important application value in maize male sterility line cultivation, sterile hybrid seed production and molecular marker-assisted selection.

The invention belongs to the technical field of molecular biology and plant molecular breeding, and particularly relates to a mustard core SNP molecular marker set and a screening method and application thereof. The invention provides a core SNP marker set (33) which is subjected to whole genome re-sequencing screening and experimental verification and is suitable for mustard germplasm resource identification and genetic analysis for the first time. The marker set is high in polymorphism and good in stability, covers the whole genome and can effectively distinguish different leaf mustard germplasms. By utilizing the core marker set and the matched KASP primer, the genetic typing of the leaf mustard germplasm resources can be quickly and accurately performed with high throughput, and the defects that the traditional morphological identification is time-consuming, labor-consuming and poor in accuracy are overcome. The marker set can be used for analyzing the genetic diversity, the population structure and the phylogenetic relationship of the leaf mustard germplasm resources, and a molecular basis is provided for collection, preservation and evaluation of the germplasm resources and breeding parent matching.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to sheep fecundity and application of the SNP molecular marker. The SNP molecular marker comprises an SNP1 (Single Nucleotide Polymorphism) and / or an SNP2; the SNP1 is located at a base at the 115747587 site of a chromosome 6 of a sheep reference genome AR-UIRamV2.0 version, and the polymorphism of the SNP1 is T / G; the SNP2 is located at a basic group at the 41419566th site of a chromosome 22 of a sheep reference genome AR-UIRamV2.0 version, and the polymorphism of the SNP2 is A / G. According to the scheme, the SNP molecular marker related to sheep fecundity is provided, sheep with the high fecundity character can be efficiently and accurately identified and screened out, and the marker is high in typing quality, single in copy and high in polymorphism and can be used for sheep fecundity molecular marker assisted breeding.

The invention belongs to the technical field of animal molecular markers, and particularly relates to an SNP molecular marker for beef cattle flavor character breeding and application of the SNP molecular marker. The invention proposes that genotype polymorphism of 84181318bp and 84181699bp on chromosome 3 of an international bovine reference genome AR-UCD1.2 version is related to the character of the content of the beef arachidonic acid for the first time, the genotype polymorphism can be used for evaluating the content of the beef arachidonic acid, and beef cattle with specific genotypes at the two sites has relatively high content of the beef arachidonic acid. The site can be used as a molecular marker for evaluating the content of the arachidonic acid in the beef cattle, early prediction and auxiliary screening of beef cattle individuals with excellent meat quality and flavor characters, and a theoretical basis and a technical support are provided for breeding of the beef cattle with high content of the arachidonic acid in the beef cattle.

The invention belongs to the technical field of plant molecular markers, and particularly discloses an Indel molecular marker related to the salt tolerance of brassica napus and application of the Indel molecular marker. The marker is located at the position of an A01 chromosome scafoldA0133077947 of a brassica napus reference genome ZS11.v10, and the genotype of a polymorphic site is CTAA or C. The invention further discloses a method for identifying the marker of the brassica napus. Large-scale group phenotypic association analysis proves that the molecular marker is remarkably associated with the salt tolerance of the brassica napus in the germination period, and when the polymorphic site genotype is CTAA, the salt tolerance in the germination period is high; when the genotype of the polymorphic site is C, low salt tolerance in the germination period is shown. The invention finds that the polymorphic site is obviously associated with the salt tolerance of the brassica napus for the first time, provides a powerful molecular tool for solving the problems of difficulty in phenotype identification, long period and low efficiency in salt tolerance breeding of the brassica napus, can accurately screen salt-tolerant materials in the early stage, and greatly accelerates the breeding process of new varieties of the salt-tolerant brassica napus.

The invention discloses a molecular marker primer group and a kit for identifying a macadamia nut variety Diishi No.1 and application of the molecular marker primer group and the kit. The molecular marker primer group is composed of five pairs of primers, and each pair of primers is composed of an upstream primer and a downstream primer. An upstream primer of the first pair of primers is as shown in SEQ ID NO: 1 in a sequence table, a downstream primer of the first pair of primers is as shown in SEQ ID NO: 2 in the sequence table, an upstream primer of the second pair of primers is as shown in SEQ ID NO: 3 in the sequence table, a downstream primer of the second pair of primers is as shown in SEQ ID NO: 4 in the sequence table, an upstream primer of the third pair of primers is as shown in SEQ ID NO: 5 in the sequence table, and a downstream primer of the fourth pair of primers is as shown in SEQ ID NO: 6 in the sequence table. The downstream primer of the third pair of primers is shown as SEQ ID NO: 6 in the sequence table, the upstream primer of the fourth pair of primers is shown as SEQ ID NO: 7 in the sequence table, and the downstream primer of the fourth pair of primers is shown as SEQ ID NO: 8 in the sequence table. The molecular marker primer group can be used for accurately identifying the macadamia nut variety Diishi No.1.

The invention discloses a corn crossbreeding intelligent matching system and method based on artificial intelligence, and the method comprises the steps: collecting molecular marker genotype data and phenotype data of candidate parents, calculating the genetic distance between the parents, and constructing a genetic distance matrix; analyzing the phenotypic complementarity degree of the parent pair, and calculating a phenotypic complementarity index; constructing a heterosis prediction model based on genetic distance and phenotype complementarity, and predicting the yield heterosis value of the candidate hybrid combination; evaluating the environmental adaptability and resistance comprehensive score of the candidate combination; and adopting a Pareto multi-objective optimization strategy to screen an optimal matching scheme. According to the method, molecular marker data and phenotype data are integrated, a heterosis prediction model considering a genetic distance nonlinear effect is constructed, collaborative optimization of multiple breeding targets such as yield, resistance and environmental adaptability is achieved, the prediction accuracy is improved by about 20% compared with that of a traditional method, and the method is suitable for large-scale popularization and application. And an intelligent and precise decision support tool is provided for corn crossbreeding.