7502 results about "Molecular marker" patented technology

A sheep single nucleotide polymorphism (SNP) molecular marker and use thereof in detecting resistance to brucellosis in sheep are provided, relating to the technical field of molecular marker-assisted breeding. A sheep SNP molecular marker is provided, where the sheep SNP molecular marker is associated with resistance to brucellosis in sheep, and has a nucleotide sequence shown in SEQ ID NO: 1 in which a T / C polymorphism is located at 101st bp. The sheep SNP molecular marker can accurately detect a resistance level of sheep to the brucellosis, and is used for the selection of brucellosis-resistant sheep as well as molecular marker-assisted breeding of the resistance to brucellosis in sheep. The sheep SNP molecular marker effectively improves a breeding efficiency of sheep with brucellosis resistance.

The invention belongs to the technical field of genetic breeding, and particularly relates to a molecular marker influencing sheep weight traits and application thereof, and the molecular marker comprises a site I or a site II. The nucleotide sequence of the molecular marker containing the site I is as shown in SEQ ID NO.1, and mutation from T to C occurs at the 101bp position in the SEQ ID NO.1; the nucleotide sequence of the molecular marker containing the site 2 is as shown in SEQ ID NO.2, and mutation from A to G occurs at the 101bp position in the SEQ ID NO.2. According to the molecular markers influencing the sheep weight traits, the marker I and the marker II can be used for identifying the sheep weight traits, and the offspring weight can be increased by selecting TT or AA genotype individuals as male parents or female parents.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to growth traits of litopenaeus vannamei and application of the SNP molecular marker, and belongs to the technical field of biology. The SNP molecular markers 1-3 are remarkably related to the growth traits of the litopenaeus vannamei, genomic DNA of muscular tissue of the litopenaeus vannamei to be detected is extracted and then serves as template DNA to be subjected to PCR amplification and sequencing, the genotypes of the three molecular markers are determined, and the litopenaeus vannamei with obvious growth advantages can be rapidly screened out. The molecular marker can accelerate the breeding process of the improved variety of the litopenaeus vannamei, and provides a theoretical basis for mining application of related genetic markers in litopenaeus vannamei molecular marker-assisted breeding.

The present invention relates to the field of tobacco breeding, and more particularly to molecular markers for screening for spotted wilt-resistant tobacco plants free of linkage drag and their applications. A method for screening spotted wilt-resistant tobacco plants or germplasm with reduced linkage drag is provided, comprising: (a) screening for spotted wilt-resistant tobacco plants or germplasm and isolating nucleic acid thereof; (b) detecting a first linkage drag locus marker and / or a second linkage drag locus marker in the isolated nucleic acid; the first linkage drag locus marker comprising the NaChr4_2M marker shown in SEQ ID No. 10 and / or the NaChr4_8M marker shown in SEQ ID No. 11; the second linkage drag locus marker comprising the NaChr3_62.6M marker shown in SEQ ID No. 7 and / or the NaChr3_64.6M marker shown in SEQ ID No. 8; and (c) selecting a spotted wilt-resistant tobacco plant or germplasm that does not contain the first linkage drag locus marker and / or the second linkage drag locus marker. This method can be used to obtain spotted wilt-resistant tobacco plants free of linkage drag, providing superior varieties with high spotted wilt resistance for tobacco leaf production.

The invention belongs to the technical field of molecular biology, and particularly relates to a molecular marker related to the number of branches of medicago sativa and application of the molecular marker. The invention solves the technical problems that the traditional new variety breeding of alfalfa with a large number of branches is single plant selection according to the number of branches of breeding offspring, and the method is time-consuming, labor-consuming and low in accuracy. The technical scheme of the invention is a molecular marker related to the number of branches of medicago sativa. The specific information is as follows: chromosome: chr2; the position is 57649197; sNP (Single Nucleotide Polymorphism) typing: C / A. The invention also designs a primer combination aiming at the molecular marker, and the primer combination can be used for directly and specifically distinguishing and detecting A or C basic groups of SNP mutation sites.

The invention discloses a litopenaeus vannamei body length growth trait associated SNP molecular marker, a detection primer and application thereof. The sequence of the SNP molecular marker is as shown in SEQ ID NO.1, the SNP site is located at the 282 site from the 5'end of the sequence as shown in SEQ ID NO.1, and the polymorphism of the SNP molecular marker is T / C type. The SNP locus can be used for body length screening of litopenaeus vannamei in litopenaeus vannamei assisted breeding, and a primer designed according to the sequence of SEQ ID NO.1 or a kit prepared from the primer can be used for detecting the SNP locus. The SNP molecular marker Chr3711743678 provided by the invention can be used for assisting in selecting rapid growth parents, and the CC genotype in the SNP molecular marker is used for selecting the parents, so that the growth speed of breeding populations can be increased, an innovative tool is provided for molecular breeding of litopenaeus vannamei, the breeding period is shortened, and industrial development and efficiency improvement are assisted.

The invention is suitable for the technical field of tumor treatment and protection, and particularly relates to an intelligent classification prediction method, system and equipment for tumor treatment and protection, and the method comprises the steps: obtaining tumor-related molecular marker detection values and clinical diagnosis and treatment features of all target patients, and comprehensively integrating the multi-dimensional medical information of the patients; according to the relevance of molecular markers and clinical features, a scientific and quantitative multi-dimensional prognosis layering index set is constructed, and an accurate layering evaluation framework is formed; inputting the medical data into a pre-trained multilayer neural network model, and generating a high-resolution tumor characterization map through a cross-modal feature fusion technology; based on the dynamic matching of the atlas and the hierarchical index set, the hierarchical contribution degree parameter and the prognosis risk score are calculated in combination with the random forest model, the precise quantitative evaluation of the condition of the patient is completed, a high-credibility quantitative basis is provided for clinical decision making, the over-treatment risk is effectively reduced, and the prognosis management effect of the patient is improved.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker site related to wheat cold resistance and application of the SNP molecular marker site. The molecular site is located at the 101st basic group from the 5'tail end of a sequence as shown in SEQ ID NO.1, and the polymorphism is C / T. The molecular marker related to wheat cold resistance in the wintering period provided by the invention can be used for detecting whether a wheat variety or strain contains a cold-resistant site or not, and has very high value for cold-resistant breeding of wheat, and meanwhile, the developed KASP molecular marker can be used for greatly accelerating the breeding process of the wheat cold-resistant variety.

The invention provides a specific single nucleotide polymorphism (SNP) molecular marker for sex identification of cobia and application of the specific single nucleotide polymorphism (SNP) molecular marker. Based on whole genome high-throughput sequencing data of male and female cobia, after deep comparative analysis, the specific molecular marker related to the sex of cobia is screened for the first time, and the primer pair designed for the marker can be used independently or used in a combined mode and is high in flexibility. The method has relatively high judgment accuracy, so that the sex of cobia can be quickly and accurately identified, and the method has a very good application prospect.

The invention provides a molecular marker related to salt-tolerant rapid growth traits of grass carps and application of the molecular marker, and belongs to the technical field of molecular markers. According to the invention, a group of molecular markers related to salt-tolerant and rapid growth traits of grass carp are obtained through GWAS analysis and screening. The invention also develops a group of amplification primers based on the molecular marker, the amplification primers can realize screening and / or identification of salt-tolerant fast-growing grass carp germplasm, and the more the enriched dominant genotypes are, the higher the growth trait index is. Compared with the prior art, the molecular marker provided by the invention can be used for accurately and efficiently identifying high-quality grass carp individuals with salt-tolerant and fast-growing characters, and the salt-tolerant breeding progress of grass carp is accelerated.

The invention relates to the technical field of molecular biology and molecular markers, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker located on a porcine chromosome 14 and related to porcine reproductive traits and application. The SNP site of the SNP molecular marker located on the No.14 chromosome of the pig and related to the reproductive traits of the pig corresponds to the Cgt at the 105043272th site on the No.14 chromosome of the international pig reference genome version 11.1; t mutation is carried out, and the polymorphism of the basic group at the site affects the porcine reproductive traits; wherein the reproductive performance of pigs with CC and TC genotypes is superior to that of pigs with TT genotypes. By optimizing the dominant alleles of the SNP molecular marker, the frequency of the dominant alleles can be increased generation by generation, the reproductive performance of pigs is improved, and the genetic improvement progress of the pigs is accelerated, so that the economic benefit of pig breeding is effectively improved.

The invention provides an SNP (Single Nucleotide Polymorphism) site combination for identifying streptococcicosis resistance of tilapia and application of the SNP site combination. DNAs of tilapia tail fin tissues attacked by streptococcus agalactiae are re-sequenced, resistant and sensitive individuals of a tilapia population are screened, GWAS analysis is carried out, SNPs sites related to tilapia streptococcus agalactiae disease resistance are identified, functional gene annotation and enrichment analysis are carried out, two SNP sites LG1320881657 and LG1320548718 are screened, and the SNP sites related to tilapia streptococcus agalactiae disease resistance are obtained. The GG genotype of the LG1320881657 and the TT genotype of the LG1320548718 are the dominant genotypes related to the streptococcus agalactiae disease resistance of the tilapia mossambica. The tilapia mossambica resistant to streptococcosis is successfully screened by utilizing the two SNPs sites, effective SNPs are provided for developing tilapia mossambica anti-streptococcosis molecular marker-assisted breeding, excellent characters of parents are ensured, and the breeding efficiency is improved.

The invention discloses a sugarcane germplasm resource evaluation and breeding method for smart agriculture. The sugarcane germplasm resource evaluation and breeding method comprises the following steps: step 1, collecting materials with wide genetic diversity; establishing an intelligent incubator and a greenhouse, and monitoring environmental parameters by using an Internet of Things sensor; 2, performing data acquisition on the related germplasm resources by using an unmanned aerial vehicle, a robot and a near infrared spectrum technology, and constructing a phenotype database for data management; meanwhile, genotype identification is carried out by combining molecular marker-assisted selection and genome selection technologies, molecular markers of key genes are mined, and the genetic value of related germplasm is evaluated; step 3, constructing a hybrid combination prediction model by using an AI algorithm, optimizing parent matching of the related germplasm and the cultivated sugarcane, monitoring and collecting the filial generation at the same time, and analyzing and screening in combination with smart agriculture; 4, demonstration planting is conducted on the bred excellent sugarcane strain, and application of the excellent strain is improved through government-enterprise-scientific research institution collaborative popularization and in combination with farmer technical training.

The invention discloses a molecular marker for auxiliary screening of anti-freezing wheat germplasm and application of the molecular marker, and relates to the technical field of biology, in particular to a method for screening or auxiliary screening of wheat with different freezing resistance, which comprises the following steps: detecting whether the genotype of wheat to be detected is qFDG-7A. 3a or qFDG-7A. 3b, and the freezing resistance gt of the wheat with the genotype qFDG-7A. 3a; the freezing resistance of the wheat with the genotype qFDG-7A. 3b; the wheat of which the genotype is qFDG-7A. 3a is wheat of which the genotype is AA homozygous based on an A129C SNP (Single Nucleotide Polymorphism) site; the wheat of which the genotype is qFDG-7A. 3b is wheat of which the genotype is CC homozygous based on an A129C SNP (Single Nucleotide Polymorphism) site; the A129C SNP site is the 129th nucleotide from the 5'tail end of SEQ ID NO: 1 in a wheat genome. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of wheat germplasm or breeding progeny materials with high anti-freezing capacity.

The invention provides an Indel molecular marker primer pair, a kit and a method for identifying Yichang orange, and belongs to the technical field of biochemistry, the Indel molecular marker primer pair comprises at least one of an InDel-2 primer pair and an InDel-3 primer pair; the sequences of the InDel-2 primer pair are as shown in SEQ ID No. 1 and SEQ ID No. 2, and the sequences of the InDel-3 primer pair are as shown in SEQ ID No. 3 and SEQ ID No. 4. The Indel molecular marker primer pair or the kit disclosed by the invention can specifically recognize the Yichang oranges, realizes efficient and accurate identification of the Yichang oranges and filial generations thereof, and has wide application prospects in the aspects of citrus germplasm resource protection, variety right and interest maintenance, breeding selection, market supervision and the like. The method for identifying the Yichang oranges can accurately identify the Yichang oranges and the filial generations of the Yichang oranges, has high specificity, economical efficiency and high efficiency, is an accurate, economical and efficient hybrid identification means, can effectively identify the variety of the Yichang oranges and the hybrids of the Yichang oranges, and provides powerful technical support for scientific research and breeding work.

The invention provides application of a Pto-C3H35 gene haplotype in evaluation of poplar lignin content, and belongs to the technical field of molecular markers. According to the invention, the haplotype of the Pto-C3H35 gene is determined according to the nucleotides of SNP1, InDel1, InDel2, InDel3 and SNP2. The invention provides a primer group and a kit for determining the gene haplotype as well as a method for determining the gene haplotype, the lignin content of the poplar can be accurately judged by determining the genotype combination of the five sites, and superior plants with low lignin content are accurately and efficiently screened in the early growth stage of the poplar; the method is applied to new variety breeding of industrial poplar artificial forests, and effectively shortens the breeding period.

The invention discloses a soybean SNP molecular marker combination, a chip and application thereof, and relates to the field of soybean molecular genetic breeding. The SNP molecular marker combination developed by the invention is uniformly distributed on 20 chromosomes of a soybean whole genome, and the accuracy of related research application such as gene positioning can be improved. The SNP molecular marker combination provided by the invention is rich in information amount and has important value in variety identification, correlation analysis and molecular marker-assisted breeding. On the basis, detection products such as SNP typing chips, kits and the like which are high in capture rate, high in density and high in polymorphism and are uniformly distributed in a genome can be developed.

The invention discloses an SNP molecular marker related to the conception rate of young cows, a detection product and application of the SNP molecular marker, and belongs to the technical field of dairy cow breeding markers. The SNP molecular marker is chr6: g. 103355581Cgt, and the SNP molecular marker is a molecular marker. The method comprises the following steps of: taking a bovine reference genome version UMD3.1 as a standard for a coordinate position, numbering Assessment in NCBI (National Center of Biotechnology Information) as GCF000003055.6, and numbering a chromosome as AC 000163.1; the young cows are cows which are 12-24 months old and do not lay calves. By detecting the genotype of the SNP site, the conception rate condition can be effectively judged, parent individuals with the higher young cow conception rate can be screened, early selection of genotype individuals with the high young cow conception rate is achieved, the frequency of the genotype with the high young cow conception rate in a dairy cow group is increased, and the reproduction rate of the dairy cow group is increased; the service life of cows is prolonged, the treatment cost is saved, the breeding cost is reduced, and the pasture income is increased.

The invention relates to the technical field of gene engineering, and discloses a KASP marker related to thousand seed weight of wheat and application of the KASP marker. The SNP locus is located at the 33397938bp position of the wheat 1A chromosome, and the polymorphism is C / T. Through phenotypic difference and exon capture analysis, the SNP site closely related to the thousand seed weight is determined, and a specific KASP primer combination is designed, so that rapid and accurate identification of the wheat thousand seed weight is realized. The application of the technology has significant significance in accelerating high-yield breeding of wheat. According to the method, the selection efficiency is improved, the breeding period is shortened, and wheat varieties with ideal thousand seed weight can be quickly identified and cultivated. By using the KASP primer combination, an effective tool is provided for molecular marker-assisted selection of wheat yield-related traits, and the overall yield and quality of crops can be improved.

The invention relates to the technical field of molecular marker development and molecular marker assisted breeding, and particularly discloses a KASP molecular marker closely linked with a wheat drought resistance related gene TaRPL36-8, a primer and application, the nucleotide sequence of the KASP molecular marker is shown as SEQ ID NO.1, and T / C polymorphism exists at the 201bp position of the sequence. By detecting the KASP molecular marker, the drought-resistant variety of wheat is identified, and technical support is provided for wheat breeding.

The invention belongs to the technical field of aquatic biotechnology and molecular marker-assisted breeding, and particularly relates to a cynoglossus semilaevis vibrio harveyi-resistant KASP molecular marker combination and application thereof in cynoglossus semilaevis disease-resistant breeding. The KASP molecular marker combination comprises three SNP (Single Nucleotide Polymorphism) loci which are respectively positioned at 17314099, 17325496 and 17355989 loci of the 14 # chromosome of the cynoglossus semilaevis, when the genotypes of the three SNP loci are respectively CC, AA and GG, the disease resistance of the cynoglossus semilaevis is high, and the invention also provides a primer for detecting the genotype polymorphism of the SNP loci. By utilizing the KASP molecular marker combination, the vibrio harveyi resistance genotype in a cynoglossus semilaevis breeding population can be quickly detected, and disease-resistant individuals are screened, so that the disease-resistant breeding efficiency of the cynoglossus semilaevis is greatly improved, and a new variety of the cynoglossus semilaevis with strong disease resistance can be quickly cultivated.

The invention relates to the technical field of molecular markers, in particular to KASP primers of 112 carya illinoensis varieties, an SNP molecular marker, a fingerprint spectrum and application of the KASP primers and the SNP molecular marker. By adopting the technical scheme provided by the invention, the carya illinoensis fingerprint spectrum can be drawn, is used for accurate identification of carya illinoensis varieties, and has important significance on germplasm resource identification and molecular marker breeding of carya illinoensis.

The embodiment of the invention discloses a KASP molecular marker, a primer pair and a kit for identifying the dwarf character of muskmelon and application of the KASP molecular marker, relates to the technical field of molecular markers, and aims to promote gene positioning and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on the dwarf character of muskmelon. The method for identifying the dwarf character of the muskmelon comprises the following steps: extracting genome DNA of a target muskmelon, taking the genome DNA of the target muskmelon as a template for PCR amplification, and performing fluorescence detection based on a primer of a KASP marker K1069930 to obtain a gene type of the target muskmelon, on the basis of the detected gene type, the character of the target muskmelon is judged; the KASP marker K1069930 is designed according to an SNP (Single Nucleotide Polymorphism) site at Chr07: 1069930, and the nucleotide sequence of the SNP site is shown as SEQ ID NO. 1. The invention also discloses a kit for detecting the KASP marker K1069930 and a kit for detecting the KASP marker K1069930. The identification method provided by the invention can be more efficiently applied to breeding work of dwarf muskmelon germplasm.

The invention discloses a KASP molecular marker for identifying soybean hundred-grain weight and application thereof, and relates to the technical field of biology and application of an SNP locus, the SNP locus takes a soybean Wm82. A2. V1 genome sequence as a reference genome, the SNP is the 16321243th SNP on a soybean 11 # chromosome, and the SNP is the 16321243th SNP on the soybean 11 # chromosome. Corresponding to the 20th basic group from the 5'terminal of the sequence shown in SEQ ID NO: 1, when the locus is CC homozygosis, the corresponding genotype is A; when the locus is TT homozygous, the corresponding genotype is B; according to the application, soybeans with different hundred-grain weights are screened or screened in an auxiliary mode, and the different hundred-grain weights of the soybeans are as follows: the homozygous soybeans with the genotype A are higher than or candidate to be higher than the homozygous soybeans with the genotype B. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of soybean germplasm or breeding progeny materials with high hundred-grain weight.

The invention belongs to the technical field of crop genetic breeding, and particularly relates to a rice hybrid male sterility site HMS10 co-segregation molecular marker and application thereof. The method comprises the following steps: hybridizing and backcrossing an anti-thermo-sensitive type genic male sterile line Yannong S serving as a female parent with a normal fertile material Yannong HB (serving as a recurrent male parent), finely positioning to a site HMS10 for controlling hybrid male sterility of rice in a physical interval of-60kb of a chromosome 10 of the rice by utilizing a high-generation backcross large population, and developing a co-separation molecular marker IDHMS10. The molecular marker IDHMS10 is utilized, through a molecular breeding strategy, a positive temperature-sensitive male genic male sterile line Quan 211S with excellent comprehensive agronomic traits is transformed into a practical rice hybrid male sterile line Quan 211HS with fertility not affected by light and temperature, and the IDHMS10 is used for purity identification of the hybrid male sterile line, so that the molecular marker IDHMS10 has great significance in solving the seed production risk of two-line hybrid rice and improving the yield of the hybrid male sterile line. And the method has important significance in enriching practical sterile resource types and the like.

The invention provides a KASP molecular marker related to a corn ear height character under a drought condition and application of the KASP molecular marker, and belongs to the technical field of molecular genetics. The SNP marker site related to the ear height character under the drought condition and having polymorphism is obtained through whole genome association analysis, the SNP marker site refers to a corn B73 RefGenv4 genome, when nucleotide polymorphism T / C exists at the position 147243591 of a 10 # chromosome, the SNP marker site is a dominant character, and nucleotide sequences used for amplifying a KASP molecular marker primer group are shown as SEQ ID NO.1-3. Gene typing identification can be rapidly, simply and efficiently carried out by utilizing a KASP typing technology, enzyme digestion and electrophoresis are not needed, accurate identification and selection can be carried out in a seedling stage, a large amount of breeding workload can be reduced, the period of high-ear-position breeding selection can be shortened, and the method can be used for high-ear-position molecular marker-assisted breeding of corn.

The invention discloses a sheep whole genome SNP molecular marker, which comprises 50000 SNP molecular markers, the SNP molecular marker is composed of 5235 functional SNP molecular markers and 44765 SNP molecular markers, the functional SNP molecular markers mainly select variation sites related to economic traits (such as growth, slaughtering, meat quality, breeding, lamb and the like) of sheep, and the variation sites are selected from the variation sites. And the background SNP molecular marker is used for improving the typing precision and polymorphism coverage of the chip, so that the applicability of the chip in a large-scale group is ensured.

The invention provides an SNP molecular marker combination for pop corn whole genome typing, a detection chip and application, and relates to the technical field of molecular breeding. The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker combination for genotyping of the whole genome of the pop corn, a genotyping object comprises 6419 SNP loci, the SNP markers uniformly cover the whole corn reference genome, and a high-quality molecular marker can be provided for map-based cloning of a pop corn specific agronomic trait control gene. Meanwhile, different types of corn can also be subjected to genotyping. Burst corn germplasm resources are narrower than other types of corn germplasm resources, and the chip can overcome the defect that an existing chip developed based on grain corn diversity is low in diversity in pop corn genotyping.

The invention discloses a molecular marker related to Chinese pepper fruit orientation, a primer combination, a kit and application. In combination with comparative genomics and re-sequencing analysis, a key variation site related to the orientation of the Chinese pepper fruit is successfully positioned on the upstream of the Up gene, a molecular marker primer is designed based on the variation site, and it is verified that the variation can influence the orientation of the fruit by using a Chinese pepper population. Through development and utilization of the molecular marker, the orientation of the Chinese pepper fruits can be accurately identified in the seedling stage. Different from a traditional linkage marker, the molecular marker is located on the upstream of the gene instead of the linkage marker, so that the molecular marker has higher identification effect and accuracy.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker combination for constructing an oat DNA (Deoxyribose Nucleic Acid) fingerprint spectrum and application, the SNP molecular marker combination is developed and obtained based on an SLAFseq technology, and has the characteristics of uniformly covering an oat genome, being high in polymorphic information content and being suitable for typing. Compared with other traditional molecular marker technologies, the SNP marker provided by the invention has higher stability, is not easily influenced by experimental conditions and environmental factors, and can be widely applied to oat germplasm resource identification, genetic diversity analysis, DNA fingerprint construction and molecular marker-assisted breeding. The DNA fingerprint spectrum constructed on the basis of the SNP molecular marker can more comprehensively reflect genetic information of a biological individual or population, and is of great significance to research of genetic diversity, evolutionary relationship and the like of species. Therefore, the method can provide technical reference for popularization of oat varieties, property right confirmation and protection, purity identification of oat seed production and the like.