4080 results about "Molecular marker" patented technology

The invention discloses a KASP molecular marker primer combination for detecting peanut late spot disease resistance and application of the KASP molecular marker primer combination, and belongs to the technical field of peanut molecular breeding. The molecular marker linked with the late spot resistance character can be used for detecting the genotype of peanuts so as to judge whether a material has late spot resistance or not, through high-throughput genotyping detection, manpower and material resources are greatly saved, the detection is not influenced by environmental factors, the result is accurate and reliable, and the molecular marker can be used for early screening of peanut late spot resistance varieties and can be applied to early screening of peanut late spot resistance varieties. The breeding period can be shortened and the breeding efficiency is improved.

The invention discloses a molecular marker closely linked with wheat flag leaf width major QTL and application of the molecular marker in prediction of wheat flag leaf width, and belongs to the technical field of wheat molecular biotechnology and breeding. Wherein the molecular marker is 4BFLW-290, the nucleotide sequences of the molecular marker are as shown in SEQ ID NO: 3 and SEQ ID NO: 4, the specific primer pair of the molecular marker is 4BFLW-290-F / R, and the nucleotide sequences of the specific primer pair are as shown in SEQ ID NO: 1 and SEQ ID NO: 2. The molecular marker 4BFLW-290 provided by the invention fully embodies the flag leaf width major QTL of the wheat variety (line), can quickly and accurately predict whether the wheat variety (line) has the flag leaf width major QTL or not, and provides excellent gene resources and selection tools for wheat yield trait molecular breeding.

The invention relates to the technical field of plant breeding, in particular to a molecular marker related to oil content and protein content of soybean and application of the molecular marker. The molecular marker is constructed on the basis of SNP sites; based on a genome version number Wm82. A2. V1, the SNP site is located at the 8605814th site of the No.8 chromosome of the soybean, and the polymorphism is A / G. The application comprises the following steps: (1) predicting or detecting the oil content or protein content of soybeans; (2) identifying or cultivating soybean varieties with high oil content or protein content; (3) soybean molecular marker assisted breeding; (4) soybean variety improvement related to oil content or protein content; and (5) soybean germplasm resource improvement. The molecular marker related to the soybean oil content and the protein content is obtained through research and screening, and the molecular marker can be applied to cultivation of soybean varieties with high oil content and high protein content and has important value in the field of soybean breeding.

The invention belongs to the technical field of pepper planting, and discloses a pepper multi-branch gene CaBr1 linked KASP molecular marker as well as a primer, a kit and application thereof, the KASP molecular marker takes pepper S8 (zhangshuang) as a reference genome, and the single nucleotide polymorphism at the 103175415th basic group on the No.8 chromosome is subjected to basic group G-to-A replacement. A chromosome region closely linked with multiple branches of the pepper is obtained by using a BSA (Bovine Serum Albumin) population positioning method, a molecular marker is developed in a candidate region to determine a candidate gene CaBr1, a KASP molecular marker is screened according to base mutation of the candidate gene, and the marker is used for carrying out genotype identification on 90 randomly sampled single plants of an F2 population, and the coincidence rate reaches 100%. The result not only contributes to pepper branch phenotype identification and assistant breeding, but also lays a foundation for map-based cloning of the branch gene and analysis of a molecular mechanism of pepper plant type regulation.

The application provides a wheat flour wet gluten content main effect site and SNP marker and application, and belongs to the wheat breeding technical field, and mainly provides SNP molecular markers Marker45889, Marker45896, Marker45348, Marker45403, Marker45397 and Marker45431, and the SNP site provided by the application has important significance for detecting and breeding wheat flour high FWG varieties, lines and breeding materials, and molecular markers can be developed according to the SNP site, so that the wheat quality breeding efficiency is accelerated.

The invention belongs to the technical field of genetic breeding, and particularly relates to application of a molecular marker for breast circumference character breeding of grassland short-tailed sheep, the molecular marker is at least one of the following (1)-(3): (1) a nucleotide sequence as shown in SEQ ID NO.1, nucleotide at the 101bp site is A or G; (2) a nucleotide sequence as shown in SEQ ID NO.3, wherein the nucleotide at the 101bp site is C or A; (3) a nucleotide sequence as shown in SEQ ID NO.5, wherein the nucleotide at the 101bp site is T or C; the application refers to any one of the following steps: identifying the chest circumference of the grassland short-tailed sheep; the chest circumference of the offspring of the grassland short-tailed sheep is improved. By detecting the genotype of the molecular marker and selecting a dominant genotype individual as a parent, the average chest circumference of offspring can be effectively improved, and a reliable tool is provided for realizing molecular assisted breeding and precise genetic improvement of the chest circumference character of the grassland short-tailed sheep.

The invention discloses a molecular marker related to thousand seed weight of wheat, a detection primer and application of the molecular marker and the detection primer, and belongs to the technical field of molecular marker-assisted breeding. The nucleotide sequence of the molecular marker TaDTX50-STS is as shown in SEQ ID NO. 1; basic groups from the 502 site to the 661 site of the sequence as shown in SEQ ID NO. 1 have insertion / deletion mutation. The InDel molecular marker related to the thousand seed weight character in a wheat genome is identified and named as TaDTX50-STS, and by detecting the polymorphism or genotype of the InDel molecular marker, the InDel molecular marker can be used for identifying or assisting in identifying the thousand seed weight character of wheat, assisting in breeding dominant varieties with high thousand seed weight of wheat and improving the wheat breeding efficiency.

The invention discloses a biomarker for evaluating litchi quality and a detection method, and relates to the technical field of biology. The marker is an SNP (Single Nucleotide Polymorphism) molecular marker, and the nucleotide sequence of the marker is as shown in SEQ ID NO.1-3. The molecular marker disclosed by the invention can be used for evaluating the quality of litchis, particularly evaluating the fructose content. Meanwhile, when the gene is applied to molecular marker assisted selection (MAS) and genome selection (GS), the genetic improvement progress of the litchi variety can be accelerated.

A molecular marker closely linked to a pepper fruit shape gene. Taking a basic version of Zhangshugang pepper as a reference gene, a single nucleotide polymorphism is present at base 162028839 of the pepper Chr02 chromosome, where a substitution from base C to A occurs. A primer and kit for identifying the molecular marker closely linked to the pepper fruit shape gene, and use thereof in identifying the pepper fruit shape or in molecular-assisted breeding. Early use of the molecular marker, the primer, and the kit in the early stage can quickly screen satisfactory plants, thereby effectively reducing the planting scale, reducing the workload of later identification, and improving the selection efficiency and accuracy. The molecular marker, the primer, and the kit are of great significance in the practice of pepper fruit shape breeding and the research on fruit shape changes and regulation mechanisms.

The invention relates to the technical field of molecular markers, in particular to an Indel molecular marker related to yellow leaves of a melon seedling growing point and application of the Indel molecular marker. The invention explores a key candidate gene MELO3C016136 for controlling the leaf color of the growth point of the muskmelon seedling, and develops an Indel molecular marker ygp2 for identifying the leaf color change of the growth point of the muskmelon seedling based on the key candidate gene MELO3C016136. The molecular marker ygp2 can be used for identifying the leaf color change character of the growth point of the muskmelon seedling, only one-time PCR amplification and polyacrylamide gel electrophoresis need to be carried out on the DNA of the plant to be detected, the identification result is reliable, the genotype of the target plant can be simply and conveniently identified, and the genotype identification result is completely consistent with the leaf color phenotype of the growth point of the seedling; important technical support is provided for creating new muskmelon germplasm with yellow seedling growing point leaves.

The invention discloses a positioning method of trachinotus ovatus growth trait related QTL (quantitative trait loci) based on 2b-RAD technology and application, the method uses 2b-RAD technology to perform sequencing on 300 trachinotus ovatus full-sib F1 generation and male and female parent individuals and develop SNP (single nucleotide polymorphism) markers, constructs a trachinotus ovatus high-density genetic linkage map, performs linkage positioning analysis in combination with 8 growth phenotype data, and finds that the trachinotus ovatus growth trait related QTL is found. 85 stable QTLs associated with the growth traits are screened out, and the stable QTLs contain 763 SNP sites. The invention also discloses an application of the method in a genetic map of trachinotus ovatus growth character positioning or molecular marker-assisted breeding of trachinotus ovatus, and the SNP molecular markers of trachinotus ovatus can be applied in genetic map construction and growth character positioning. Particularly, the method has a good application prospect in trachinotus ovatus growth trait molecular marker assisted breeding.

The invention belongs to the technical field of molecular markers, and discloses a molecular marker located in No.18 chromosome and related to the salt tolerance of soybean seeds in the germination period and application of the molecular marker. According to the present invention, the salt tolerance index of the soybean seed germination stage is determined, the stable and reliable molecular marker GmChr1856968562 closely linked with the salt tolerance ability of the seed germination stage is obtained through GWAS correlation analysis, the molecular marker is located at the 56968562 site on the No.18 chromosome of the soybean reference genome Glycine max Wm82. A2. V1, and the marker for KASP detection is developed based on the site, the invention also designs a primer group for amplifying the KASP marker. The invention further provides a method for rapidly identifying the salt tolerance of the soybean seeds in the germination period by using the primer group of the molecular marker, and the method is simple, convenient and rapid, accurate in identification result and good in popularization and application prospect.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to a single wheat spike number character and application of the SNP molecular marker, and belongs to the technical field of molecular marker breeding. The invention discloses a single wheat spike number related SNP-783 site and application thereof, the SNP site corresponds to the 783rd basic group from the 5'tail end of a sequence as shown in SEQ ID NO.1, and when the site is C / C homozygous, the corresponding genotype is A, and when the site is C / C homozygous, the corresponding genotype is B, and when the site is C / C homozygous, the corresponding genotype is C / C homozygous; when the locus is T / T homozygous, the corresponding genotype is B, and the ear number of a single plant is as follows: the wheat with the homozygous genotype A is greater than or candidate to be greater than the wheat with the homozygous genotype B. The SNP has high effectiveness and potential application value, the wheat with the high spike number per plant can be found by detecting the SNP, and the SNP has important value in research or application of cultivation of high-yield wheat varieties.

The invention discloses application of Indel marker dosage typing in detection of sweet potato soft rot resistance, and belongs to the technical field of plant genetic engineering. The Indel molecular marker related to the sweet potato soft rot resistance is obtained through screening, the nucleotide sequence of the Indel molecular marker is shown as SEQ ID NO.2, and an 8bp sequence is inserted into or deleted from the 161st site of the sequence shown as SEQ ID NO.2. Experiments find that target bands of amplification products are obtained through amplification of the Indel molecular marker and electrophoresis, dosage values of two genotypes are obtained according to gray values of the dyed electrophoretic bands, and results show that the insertion type Indel molecular marker is positively correlated with the sweet potato soft rot resistance. The method is of great significance to the dose effect research and molecular assisted breeding of sweet potato gene functions.

The invention discloses a molecular marker primer group for identifying a macadamia nut variety Yunnan No.4, a kit and application. The molecular marker primer group is composed of three pairs of primers, and each pair of primers is composed of an upstream primer and a downstream primer. An upstream primer of the first pair of primers is as shown in SEQ ID NO: 1 in a sequence table, a downstream primer of the first pair of primers is as shown in SEQ ID NO: 2 in the sequence table, an upstream primer of the second pair of primers is as shown in SEQ ID NO: 3 in the sequence table, a downstream primer of the second pair of primers is as shown in SEQ ID NO: 4 in the sequence table, an upstream primer of the third pair of primers is as shown in SEQ ID NO: 5 in the sequence table, and a downstream primer of the fourth pair of primers is as shown in SEQ ID NO: 6 in the sequence table. The downstream primer of the third pair of primers is shown as SEQ ID NO: 6 in the sequence table. The molecular marker primer group can accurately identify the macadimia nut variety Yunnan No.4.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for identifying drought tolerance of wheat, haplotypes, primers and application. Belongs to the technical field of biological breeding. Research finds that three SNP loci exist on a wheat ARF20-B gene, a wheat drought-enduring haplotype ARF20-BHapI is developed based on the SNP loci, the first locus is located at the 3616bp position of the ARF20-B gene, and the A / G nucleotide polymorphism is A; the second site is located at the 5623bp position of the ARF20-B gene, and G is in the G / A nucleotide polymorphism; the third site is located at the 9795bp position of the ARF20-B gene, and G is in the G / A nucleotide polymorphism. The wheat carrying the ARF20-BHapI haplotype has the advantage that the drought tolerance of the wheat carrying the ARF20-BHapI haplotype is higher. The invention further provides a KASP primer group for detecting the ARF20-BHapI haplotype, and successful detection of the drought tolerance of the wheat is achieved.

The invention belongs to the field of plant seed biology and rice breeding, and discloses an application of an OsBTB05 gene or an OsBTB05 protein in regulation and control of storage resistance of rice seeds and a related breeding method. In particular discloses application of an OsBTB05 gene or an OsBTB05 protein in regulation and control of the storability of rice seeds, the nucleotide sequence of the OsBTB05 gene is shown as SEQ ID NO: 1 or SEQ ID NO: 2, and the amino acid sequence of the OsBTB05 protein is shown as SEQ ID NO: 3. The invention also discloses a molecular marker-assisted selection method for breeding the high-storage-resistance rice variety and a method for reducing the storage resistance of the rice seeds. The invention proves that the OsBTB05 gene is a key factor for regulating and controlling the storability of the rice seeds, and the expression level of the OsBTB05 gene is in significant positive correlation with the storability of the seeds. A clear molecular target and a brand-new gene resource are provided for improving the seed storage resistance from a genetic source.

The invention discloses application of a KASP molecular marker in identification of light yellow flower color traits of muskmelons, and belongs to the technical field of molecular markers. According to the KASP molecular marker for identifying the light yellow flower color character of the muskmelon, a genetic segregation population is constructed by taking a dark yellow flower color muskmelon and a light yellow flower color muskmelon as parents, and a stably inherited marker site chr04: 26634798G / A is obtained through molecular marker technology identification. The marker is closely related to the light yellow flower color character of the muskmelon, and has the characteristics of strong specificity and good stability. The molecular marker provided by the invention can realize rapid genotype detection of a muskmelon material through a high-throughput genotyping system, and the identification result is accurate and reliable and is not influenced by environmental factors. The molecular marker can be applied to molecular marker-assisted selective breeding of melon flower color characters, the breeding efficiency is remarkably improved, the breeding period is shortened, the breeding cost is reduced, and an effective molecular breeding tool is provided for directional improvement of melon flower colors.

The invention discloses a KASP marker for auxiliary screening of cold-resistant wheat germplasm and application of the KASP marker, and relates to the technical field of biology, in particular to a method for screening or auxiliary screening of wheat cold-resistant germplasm, which comprises the following steps: detecting whether the genotype of wheat to be detected is genotype qFR-7A. 1a or genotype qFR-7A. 1b; the cold resistance of the wheat with the genotype qFR-7A. 1a is higher than that of the wheat with the genotype qFR-7A. 1b, or the cold resistance of the wheat with the genotype qFR-7A. 1a is higher than that of the wheat with the genotype The wheat of which the genotype is qFR-7A. 1a is wheat of which the genotype is GG homozygous based on a G101A SNP (Single Nucleotide Polymorphism) site; the wheat of which the genotype is qFR-7A. 1b is wheat of which the genotype is AA homozygous based on a G101A SNP (Single Nucleotide Polymorphism) site; the G101A SNP site is the 101 nucleotide from the 5'terminal of SEQ ID NO: 1 in a wheat genome. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of wheat germplasm or breeding offspring materials with relatively high cold resistance.

The invention belongs to the technical field of animal breeding, and particularly relates to application of a detection reagent of an SNP (Single Nucleotide Polymorphism) molecular marker related to pork tenderness. An SNP site exists at the 232bp position from the 5'end of the sequence, the polymorphism is G / A, three genotypes of GG, GA and AA are shown, G allele is dominant allele, and the meat tenderness of a GG type individual is obviously higher than that of an AA type individual. The invention further develops a reagent for detecting the SNP molecular marker, the reagent can be used for pig assisted breeding, pig breeding or pork tenderness identification, and a method for identifying the pork tenderness is developed, which is beneficial to accelerating genetic breeding and early-stage seed selection of boars with high-quality meat traits.

The invention relates to the technical field of microbiological detection and molecular biology, in particular to a specific detection primer for bifidobacterium longum subsp. Longum BBMN68 and application of the specific detection primer. The specific primer for detecting the bifidobacterium longum subsp.longum BBMN68, provided by the invention, comprises an upstream primer and a downstream primer, and nucleotide sequences are shown as SEQ ID NO.7-8. The invention also provides a kit for detecting the bifidobacterium longum subsp.longum BBMN68. The specific DNA fragment provided by the invention is a specific molecular marker of BBMN68, and the specific primer is designed aiming at the fragment sequence, so that the specific amplification of the strain can be realized, no cross reaction is caused to other related strains, the detection sensitivity is higher, and the detection requirements of samples with different concentrations can be met; the method can be used for specific detection and accurate quantification of the BBMN68 strain in yoghourt and other products, and has good practical application value.

The invention relates to the technical field of molecular biology, and discloses 74 core molecular marker sets for identifying cold resistance of pennisetum alopecuroides, a primer group, a detection reagent, a kit, a gene chip and application thereof. According to the method, core germplasm resources of pennisetum alopecuroides are systematically collected, and the GWAS population is constructed by utilizing multi-environment (different altitudes) conditions, so that wide genetic and phenotypic variation is fully covered, and a representative material foundation is laid for cold-resistant genetic analysis. In the aspect of phenotype evaluation, a method for determining the number of reviving and root cutting seedlings after natural overwintering by combining single-root double-stem-node oblique cutting planting is innovatively provided, a set of scientific and stable cold resistance and reproducibility evaluation system capable of being operated in a standardized mode is established, and the reliability and efficiency of phenotype identification are remarkably improved. The detection is not influenced by human and climate environments; the method realizes efficient and accurate early cold resistance identification of pennisetum alopecuroides, greatly improves the breeding screening efficiency and scale, and accelerates the breeding process.

The invention relates to the technical field of genetic breeding, in particular to application of a molecular marker related to daily gain traits of sheep before weaning. The molecular marker is at least one of the following (1)-(2): (1) a nucleotide sequence as shown in SEQ ID NO.1, wherein the nucleotide sequence at the 101bp site is T or C; (2) a nucleotide sequence as shown in SEQ ID NO.3, wherein the nucleotide sequence at the 101bp site is T or C. The application refers to any one of the following (1) and (2): (1) identifying the daily gain of the sheep before weaning; and (2) the daily gain of the offspring of the sheep before weaning is improved. The screened molecular marker influencing the daily gain character of the sheep before weaning can be used for identifying the daily gain character of the sheep before weaning, and the daily gain of offspring before weaning can be improved by selecting a CC genotype individual as a male parent or a female parent.

The invention relates to the technical field of crop genetic breeding, in particular to a plateau rape adaptive germplasm breeding method based on multi-character collaborative screening, which comprises the following steps of: constructing a multi-character evaluation system covering more than 20 indexes of four categories of agriculture, physiology, quality and stress resistance, and determining character weights by applying principal component analysis, grey relational analysis and analytic hierarchy process; and acquiring data by combining unmanned aerial vehicle multispectral imaging, a ground phenotype platform and a molecular marker technology, and substituting the data into the comprehensive evaluation model for germplasm screening. According to the method, the limitation of traditional single-character breeding is broken through, multi-dimensional collaborative evaluation is achieved, and the plateau rape germplasm breeding efficiency and accuracy are remarkably improved.

The invention discloses an indel molecular marker for identifying color and luster of citrus peel in a seedling stage and application of the indel molecular marker. The corresponding sites of the indel molecular marker of the citrus are positioned in chr7: 18, 398, 798-18, 399 and 289 intervals of a chromosome 7 of an SWO3.0 reference genome; the genome DNA of the citrus seedling stage plant is used as a template, the Indel molecular marker is amplified through PCR, the citrus seedling stage plant can be divided into a homozygous insertion type, a homozygous deletion type and a heterozygous type according to a PCR amplification result, and the three types are associated with the color of the citrus fruit for identifying the color of the citrus peel, so that the early screening of the seedling stage target traits of the citrus breeding population is realized, and the early screening of the seedling stage target traits of the citrus breeding population is realized. The breeding period is remarkably shortened, the breeding cost is reduced, and important technical support is provided for citrus fruit color improvement breeding.

The invention provides a closely linked molecular marker primer of a rice brown planthopper resistant major gene Bph56 as well as a marking method and application of the closely linked molecular marker primer. According to the invention, genotypes of F2 individual plants obtained by hybridizing a rice insect-resistant variety C127 (or) and KW (or) are combined with brown planthopper resistance levels of an F3 family for genetic linkage analysis, a resistance major gene Bph56 carried by the insect-resistant variety C127 is identified, and a region of the resistance major gene Bph56 is reduced to a 77kb fragment limited by markers 6M26.278 and 6M26.355 on two sides and is closely linked with a molecular marker 6M26.355. The molecular marker 6M26.355 disclosed by the invention can be used for effectively detecting whether an insect-resistant variety C127 and derived varieties (lines) of the insect-resistant variety C127 contain the major resistance gene locus or not, so that the selection efficiency of the brown planthopper-resistant character is greatly improved, and the brown planthopper-resistant rice variety (line) containing Bph56 is obtained.

The invention belongs to the technical field of molecular biology and genetic breeding, and discloses a molecular marker closely linked with soybean plant height QTL qPH1.01 and application of the molecular marker. According to the present invention, the major QTL qPH1.01 for regulating corn plant height variation is identified by using linkage analysis, the significantly associated SNP is located at the 27th, 318th and 904th bases of the first chromosome of the reference genome Glycinemaxv2.1, 6.28-11.14% phenotypic variation can be explained in the single environment, the PARMS marker developed by using the SNP is used to detect 96 soybean varieties, the typing is clear, the operation is simple and convenient, the method is suitable for soybean plant polymer breeding, and the application prospect is broad. And a new high-yield soybean variety with proper plant height and lodging resistance is cultivated.

The invention provides a beet genetic diversity analysis method based on whole genome re-sequencing. The method comprises the steps of PacBio HiFi library construction, computer sequencing, data information analysis, assembly quality evaluation and Hi-C auxiliary genome assembly. The invention further provides application, and the high-continuity beet reference genome is used for analyzing beet intraspecific genetic diversity and developing high-density molecular markers. According to the method, the high precision of HiFi, the super-long read length of Nanopore and Hi-C space interaction data are combined for the first time, the problems of repeated sequence and complex SV analysis are solved, and all-round breakthrough of genome continuity, annotation integrity, genetic diversity coverage and epigenetic integration is achieved.

The invention belongs to the technical field of molecular biology and genetic breeding, and discloses a molecular marker of a locus qSGC.A8-3 remarkably associated with glucosinolate in brassica napus seeds and application of the molecular marker. A locus qSGC.A8-3 remarkably related to the glucosinolate content of the rape seeds and a peak SNP marker Bn-A08-p12638473 of the locus qSGC.A8-3 are located at 10th, 557th and 348th basic groups of a reference genome A08 chromosome of the rape DarmorV4.1, the average additive effect is 9.6 mu mol / g, and 7.5% of phenotypic variance can be explained. When the PARMS marker designed by the SNP is used for detecting rape germplasm resources, the PARMS marker is simple and convenient to operate and clear in typing, and the average thioglycoside content of AA genotype material seeds is higher than that of GG genotype material seeds, so that the PARMS marker has a good selection effect on the thioglycoside of the rape seeds.

The invention discloses a molecular marker closely linked with a cowpea salt tolerance major QTL (quantitative trait loci) and a primer sequence of the molecular marker. The name of the cowpea salt tolerance major QTL is SC300126, a KASP type molecular marker 300126 closely linked with the cowpea salt tolerance major QTL is located at the position of 43816166 bp on a chromosome 11, the marker can be used for amplifying a material to be detected, and whether a sample carries key allelic variation for controlling salt tolerance or not is judged according to the genotype of the marker. The molecular marker and the primer provided by the invention can be used for quickly screening the cowpea salt tolerance major QTL, and compared with a traditional phenotype identification method, the molecular marker and the primer have the advantages of high detection flux, accurate and reliable result and the like, and the typing accuracy can reach 99% or above. The technology can significantly improve the breeding efficiency of the salt-tolerant variety of cowpea and shorten the breeding period.