36 results about "Selective breeding" patented technology

The invention relates to the technical field of genomics, molecular biology, bioinformatics and whole-genome selective breeding, in particular to a whole-genome liquid chip for apostichopus japonicus and application of the whole-genome liquid chip. The liquid phase chip contains sequences of background SNP loci and functional SNP loci which are used for gene analysis and are positioned on an apostichopus japonicus reference genome; wherein the functional SNP sites are associated with important economic characters of the apostichopus japonicus. The invention also discloses application of the liquid chip in apostichopus japonicus genome selective breeding, important economic character gene positioning, genetic diversity analysis, germplasm resource improvement and protection.

The invention provides an InDel molecular marker sequence related to wheat drought resistance as well as a detection method and application thereof, and belongs to the technical field of molecular markers. The invention provides a polymorphic site for forming different drought resistance of wheat, the polymorphic site only has two haplotypes: a sequence of a 724bp long fragment as shown in SEQ ID No.2 is inserted between the fragments 245-246bp as shown in SEQ ID No.1, and the haplotype is homozygous as a haplotype A; the drought resistance of to-be-detected wheat which is not inserted with the 724bp long fragment sequence shown at the site, is haplotype B homozygous and is determined to be haplotype A homozygous is higher than that of a variety determined to be haplotype B homozygous, so that the drought resistance of the to-be-detected wheat is determined to be haplotype A homozygous by detecting the condition of the polymorphic site when the wheat is subjected to molecular marker-assisted selective breeding, and the drought resistance of the to-be-detected wheat is determined to be haplotype A homozygous. The wheat with relatively high drought resistance can be found.

The invention relates to the technical field of plant molecular genetic breeding, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker closely linked with white gourd pulp malic acid major QTL (Quantitative Trait Loci) and application thereof. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO.4, and G / A mutation exists at the 26th basic group of the sequence. The molecular marker and the white gourd pulp malic acid have a close linkage marker characteristic, so that a KSAP primer pair is developed on the basis of the SNP molecular marker. Results of the specific embodiment of the invention show that the KASP molecular marker provided by the invention can be used for identifying the content of malic acid in pulp of wax gourd seeds or early-stage seedlings with true leaves, and the method is high in accuracy, low in cost and short in consumed time. Therefore, the SNP molecular marker and the KASP primer pair provided by the invention can be used for molecular marker-assisted selective breeding of white gourds, and the process of white gourd flavor, taste and quality breeding is accelerated.

The invention relates to an SNP (Single Nucleotide Polymorphism) molecular marker associated with the fatness of turbots fed with fermented soybean meal and application of the SNP molecular marker, and belongs to the technical field of molecular markers. The SNP molecular marker obviously associated with the fatness character is obtained by analyzing the fatness character of the turbots through genome-wide association analysis and comprises at least one of six SNP loci; the scophthalmus maximus molecular marker can be applied to scophthalmus maximus molecular assisted breeding, screening is conducted on the genome level through the breeding method, the breeding period can be greatly shortened, dependence on phenotypic characters is reduced, the selective breeding efficiency is improved, and the scophthalmus maximus molecular marker has good application prospects.

The invention discloses application of an SNP (Single Nucleotide Polymorphism) marker influencing the lambing number of goats. The marker is located on a PIEZO1 gene on a capa circus 18 # chromosome, the specific SNP marker site is A / C base mutation at the 236bp position of SEQ ID NO: 1 in a sequence table, and the lambing number of CC genotype and AC genotype goat individuals at the site is remarkably higher than that of AA genotype individuals. According to the present invention, the Musheng black goats are adopted as the research object, the DNA sequence of the 33rd exon of the PIEZO1 gene is subjected to PCR amplification, the sequence has an SNP variation site, the influence of the site polymorphism on the goat lambing number is analyzed, the individual Musheng black goat breeding is performed according to the influence, and the method can be used for improving the Musheng black goat lambing number so as to provide the basis for the breeding of the Musheng black goats. The breeding of a new variety (strain) taking the Muscheng black goat as a breeding material is accelerated, and a marker resource is provided for marker-assisted selective breeding of goat lambing number traits.

The invention belongs to the technical field of molecular genetics, and particularly relates to a wheat powdery mildew resistance main effect QTL, a KASP molecular marker closely linked with the wheat powdery mildew resistance main effect QTL and application of the wheat powdery mildew resistance main effect QTL. The invention provides a wheat powdery mildew resistance major QTL, the major QTL locus is located at the end part of a wheat 1A chromosome short arm and is located in a 4.4 cM genetic interval between flanking KASP markers Chr1A6275522K2 and Chr1A11524652K9, nine KASP molecules are developed, auxiliary selective breeding and screening of powdery mildew resistance wheat strains can be carried out, and a new available molecular marker is provided for screening of wheat powdery mildew resistance materials.

This invention specifically relates to the detection and application of a CASP14 gene SNP marker related to sheep horn size, belonging to the field of genetic biology. The SNP marker affecting sheep horn traits described in this invention is located at locus 7944295 on chromosome 5, and its polymorphism is G / A. This SNP marker can be used to identify sheep horn traits, i.e., whether sheep have large or small horns, and selective breeding can be carried out based on environmental adaptability.

The invention relates to the field of molecular breeding of Chinese pears, and discloses an SNP (Single Nucleotide Polymorphism) marker related to the fruit peel color of Chinese pears, the SNP marker is located at the No.8 chromosome 4163889 site of a 20-century pear PPYr1.0 genome, the fruit peel color of the Chinese pears is green when the genotype is T / T, and the fruit peel color of the Chinese pears is brown when the nucleotide at the SNP site is C / C or C / T. Compared with the prior art, the SNP molecular marker has the following advantages and effects that genome re-sequencing and whole genome association analysis (GWAS) are utilized to enrich in a gene interval related to the fruit peel color of the Chinese pears, the SNP molecular marker closely linked with the target character is developed, the SNP molecular marker can be effectively used for molecular marker-assisted selective breeding of the Chinese pears, the accuracy of character selection of the fruit peel color of the Chinese pears is improved, and the SNP molecular marker can be applied to the molecular marker-assisted selective breeding of the Chinese pears. The breeding process is accelerated.

The invention relates to the technical field of poultry genetic breeding and biology, in particular to a DGKA gene molecular marker for laying hen egg-laying persistent breeding, application and a method, and belongs to the technical field of molecular marker-assisted breeding. The molecular marker is located on a No.34 chromosome of a chicken reference genome bGalGal1. Mat.broil.GRCg7b, the 1334260th site has G / A polymorphism and is located in a 5 '-UTR region of a DGKA gene, and the SNP can be used as the molecular marker and is used for early genetic breeding of high-egg-yield persistent laying hens. The molecular marker is applied to early screening and genetic breeding of high-egg-yield persistent laying hens, breeding accuracy and breeding efficiency can be remarkably improved, phenotype determination cost is reduced, and the molecular marker has important economic value and popularization and application prospects for promoting efficient and precise development of the laying hen industry.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker combination for predicting the lysine content of fresh corn kernels and application of the SNP molecular marker combination, and belongs to the technical field of selective breeding of fresh corn. The SNP molecular marker combination provided by the invention is composed of 20000 SNP loci positioned on a corn B73 reference genome version v5. The SNP molecular marker combination provided by the invention can be used for molecular marker-assisted breeding of high-quality protein (high lysine content) fresh-eating corn, and can also be used for pyramiding breeding of multiple traits of fresh-eating sweet corn. According to the method for predicting the lysine content of the fresh corn kernels, the content of high-quality protein (lysine) of the fresh corn kernels can be predicted only by detecting the genotype of a breeding material, the method has the advantages of being clear in selection target and free of environmental influence, and meanwhile, the method can be used for predicting the lysine content of the fresh corn kernels. Prediction of the high-quality protein (lysine) content character of the grains is mainly carried out in the harvesting period of the fresh corn, and early detection can be achieved.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to sexual gland weight traits of fugu rubripes and application of the SNP molecular marker, and relates to the technical field of biology. According to the invention, association analysis is carried out in combination with the sexual gland heavy character of the fugu rubripes, and the SNP molecular marker significantly related to the sexual gland heavy character of the fugu rubripes is successfully excavated. The SNP molecular marker is used for breeding of improved gonad breeds of fugu rubripes, and compared with traditional phenotype-based selective breeding and cross breeding means, the SNP molecular marker has higher accuracy and detection efficiency and has a wide application prospect.

The present disclosure relates at least in part to genetic engineering of vegetable (e.g., tomato) plants to, for example, produce heme, a molecule contributing to a meat-like flavor, and to the incorporation of male sterility in these plants to facilitate the production of F1 hybrid seeds. Described herein, in some embodiments, are methods for constructing and introducing into such plants genetic constructs containing both a heme gene and a male sterility gene, the resulting plants, and the use of these plants in hybrid seed production. The present disclosure also provides at least in part methods for enhancing lycopene content in plants, such as tomato plants, either through genetic modification or selective breeding. While introducing key genes like Psy is effective, adding multiple genes or knocking out competing pathways can lead to more substantial increases in lycopene levels. Enhanced lycopene content can provide added health benefits, including potential cancer protection, making these plants, such as tomatoes, particularly valuable in the context of functional foods. The plants may be of varieties with high lycopene content combined with genetic modifications as provided herein. The present disclosure offers a comprehensive solution for producing plants (e.g., tomatoes) with increased nutritional value.

The invention discloses a molecular marker related to the first laying day age of Jianghan chicken, a primer pair and application of the molecular marker and the primer pair, and belongs to the technical field of molecular marker-assisted selective breeding. The molecular marker is located at a first exon sequence of a chicken PCDHA1 gene and has a sequence as shown in SEQ ID NO.3, fragment repetition exists in the sequence, a base composition at the 54bp position of the sequence SEQ ID NO.3 is C or T, a base composition at the 56bp position of the sequence SEQ ID NO.3 is C or G, the polymorphism of the laying day age of the Jianghan chicken is embodied, when the base composition at the 54bp position and the base composition at the 56bp position of the sequence SEQ ID NO.3 is CC type, the laying day age of the chicken is late, and the polymorphism of the laying day age of the Jianghan chicken is determined. If the basic group at the 54bp and the basic group at the 56bp form a TG type, the chicken is early in first laying day age. When the method is used for breeding the Jianghan chickens, the breeding efficiency can be remarkably improved, the breeding period can be effectively shortened, the breeding accuracy is high, and the cost is low.

The invention belongs to the technical field of fish breeding and biochips, and particularly relates to a micropterus salmoides SNP (Single Nucleotide Polymorphism) chip and application thereof. A specific capture probe is designed based on the provided largemouth bass SNP molecular marker combination, a gene chip for whole genome genotyping of largemouth bass is constructed, the gene chip is used for accurate and comprehensive detection of whole genome genotyping of largemouth bass, and the gene chip has the advantages of high detection flux, low cost, flexible customization and the like; the method is used for germplasm resource identification, genetic diversity analysis, whole genome association analysis, important functional gene mining and selective breeding of the micropterus salmoides, and a novel and efficient tool is provided for accelerating fine variety breeding of the micropterus salmoides.

The invention relates to the technical field of molecular biology, and discloses a goat whole genome 50K breeding chip and application thereof. On the basis of whole genome re-sequencing data of goat groups (gt, 500 parts) covering domestic local and cultivated varieties and imported varieties abroad, functional marker information remarkably related to 14 economic characters such as growth and development, slaughtering, breeding, wool production and milk production of goats is screened through whole genome association analysis and selection signal analysis, and a probe combination contains 50000 SNP loci. The goat whole genome 50K breeding chip disclosed by the invention has the characteristics of strong cross-variety universality, high detection accuracy, comprehensive functional information and the like, can be widely applied to the aspects of goat genetic diversity analysis, whole genome correlation analysis, whole genome selective breeding and the like, and can be used for remarkably shortening the breeding period and reducing the typing cost.

This invention relates to the development of heat-tolerant soft-shell clam (Mya arenaria) lines for aquaculture through selective breeding and molecular genetic approaches. Two methods are employed: (1) phenotype-based selection, in which clams are exposed to natural or controlled heat shock conditions (30-33° C.) and survivors are selected across successive generations and multiple populations; and (2) marker-assisted selection (MAS), utilizing single nucleotide polymorphisms (SNPs) identified through genome-wide association studies (GWAS) to select broodstock capable of producing heat-tolerant offspring. The resulting lines exhibit enhanced survival and endurance under elevated temperatures, particularly above the species' critical threshold of 28° C. These heat-tolerant clams enable sustainable aquaculture production in Maryland and other warm, low-salinity estuaries where soft-shell clams historically failed to survive summer heat.

The invention belongs to the technical field of molecular marker-assisted selective breeding, and particularly relates to a method for breeding an anti-cyst nematode No.4 physiological race soybean variety. The invention provides a PCR (Polymerase Chain Reaction) primer combination for screening an anti-cyst nematode No.4 physiological race soybean variety, wherein the PCR primer combination comprises a first PCR primer group, a second PCR primer group and a third PCR primer group; the first PCR primer group consists of primers with nucleotide sequences as shown in SEQ ID NO: 1-3; the second PCR primer group consists of primers with nucleotide sequences as shown in SEQ ID NO: 4-6; and the third PCR primer group consists of primers with nucleotide sequences as shown in SEQ ID NO: 7-9. The invention further provides a method for breeding the cyst nematode-resistant No.4 physiological race soybean variety. When the method is used for marker-assisted selection, the efficiency and accuracy of soybean cyst nematode-resistant breeding can be greatly improved.

The invention discloses a pig SINE-RIP probe combination, a gene chip, a kit and application, the pig SINE-RIP probe combination is based on a reference genome Sscrofa11.1, has wide variety and group applicability, and detects a type of SINE-RIP structure variation markers of SINE-RIP which is large in fragment, easy to cause gene activity change and large in genetic effect. The method is suitable for various applications and researches such as pig genome selective breeding, pig germplasm resource identification and evaluation, genetic relationship analysis, genetic diversity analysis and evaluation, functional gene or locus positioning, whole genome association analysis and the like, the detection process is convenient to operate, the flux is high, the cost is low, the detection rate is high, and the typing result is accurate. The probe combination effectively supplements and even substitutes an existing SNP molecular marker system, the accuracy of genome selection can be further improved, a brand-new high-reliability tool is provided for whole genome related research and application of pigs, and the industrial application value is extremely high.

The invention belongs to the field of molecular biology, and discloses acquisition of a major QTL qRDC9.08 with corn smut resistance, and development and application of a molecular marker primer of the major QTL qRDC9.08. The invention provides a major QTL qRDC9.08 for controlling maize gall smut resistance, the major QTL qRDC9.08 is located at a ninth chromosome of maize, the locus is a major QTL locus for controlling maize gall smut resistance variation, and an excellent haplotype SNP marker closely linked with the major QTL locus is located at a 158136332th basic group of a ninth chromosome of a maize B73 reference genome and is used for identifying maize gall smut resistance variation. The explained phenotypic contribution rate of the maize gall smut resistance is 8.09%, the KASP marker designed by the SNP is used for detecting a maize inbred line group, the operation is simple and convenient, the typing is clear, and the KASP marker can be used for maize gall smut resistance molecular marker-assisted selective breeding.

The present application relates to the technical field of gene editing, and particularly relates to application of SG19 gene in regulating seed germination of rice. The present application cuts the specific site of SG19 gene by designing specific guide RNA (gRNA) and guiding Cas9 protein, so as to realize gene knockout or mutation. The CRISPR / Cas9 technology can quickly and accurately edit genes, significantly shorten the breeding cycle, simplify the breeding operation process, and reduce the workload of field test and artificial selection. Through this technology, the key genes affecting the seed germination rate can be accurately regulated. The present application reduces the expression amount of SG19 gene by the CRISPR / Cas9 technology, reduces the synthesis of ABA, and then accelerates the seed germination rate of rice, and improves the germination rate of rice seeds, thereby providing a new strategy and method for rice breeding.

The invention belongs to the field of molecular genetic breeding, and discloses an eggplant SNP (Single Nucleotide Polymorphism) molecular marker combination which comprises 1604 SNP molecular markers, and the position information is shown in the specification table 1. The invention also discloses an eggplant SNP liquid phase chip and a kit, which comprise a probe or / and a primer for detecting the eggplant SNP molecular marker combination, the invention also discloses an application of at least one of an eggplant SNP molecular marker combination, an eggplant SNP liquid phase chip or a kit in eggplant germplasm resource identification, genetic diversity evaluation, variety purity identification, eggplant genetic map construction and QTL positioning thereof, molecular marker-assisted selective breeding, whole genome selection association analysis, directional improvement or multi-gene pyramiding breeding. The eggplant SNP molecular marker combination provided by the invention is high in detection flux, large in output result information amount, high in target site detection rate and accurate and reliable in typing result.

The invention discloses three tandem repeat PAV markers related to thousand seed weight of highland barley and application of the three tandem repeat PAV markers. The three PAV markers adopt an oligonucleotide probe sleeve # 8 as a probe, detection is carried out through chromosome fluorescence in situ hybridization in the mitosis metaphase of root tip cells, variations with repetitive sequence insertion fragments are respectively named as 1H-C, 3H-A and 6H-2, and types without insertion fragments are respectively named as 1H-D, 3H-B and 6H-1. Results show that the thousand seed weights of varieties containing 1H-C, 3H-A and 6H-2 are obviously higher than those of other polymorphic chromosomes respectively. The invention provides a cytological marker for thousand seed weight detection for highland barley breeding, and is beneficial to improving highland barley marker-assisted selective breeding efficiency. The influence of chromosome structure variation on the thousand seed weight of highland barley is disclosed, and important information is provided for mining environment adaptability genes and gene clusters.

This invention relates to the field of animal molecular breeding technology, specifically providing a method related to litter size. NWD2 The SNP marker at locus 6_57554735 and its application. The SNP marker is a G / T mutation at locus 57554735 on chromosome 6 of the sheep reference genome GCF_016772045.1_ARS-UI_Ramb_v2.0. Using the SNP molecular marker provided by this invention, lambing numbers in sheep can be identified, and selective breeding can be carried out based on environmental adaptability.

The invention relates to the technical field of biomolecular breeding, in particular to an InDel molecular marker related to welsh onion leaf wax as well as a primer and application of the InDel molecular marker. Through genetic segregation population construction, a linkage candidate interval related to the welsh onion leaf wax is positioned, candidate genes and InDel in the interval are screened, and the InDel molecular marker and the primer which can be used for identifying the welsh onion leaf wax are developed. The molecular marker and the primer pair provided by the invention can be used for detecting the wax condition of the green Chinese onion leaves, can be used for rapidly screening green Chinese onion varieties without wax on the leaves and detecting the genotypes of the green Chinese onion varieties, and are used for analyzing and screening the genetic background of the green Chinese onion and promoting the breeding of hybrid varieties. The excellent site and the wax molecular marker, which are identified by the invention and have potential application value, can assist in selective breeding and have a wide application prospect.

This invention discloses the application of a SNP marker in the EGFR gene that affects weaning weight in sheep. The marker is located in the EGFR gene on sheep chromosome 19, specifically at the T / C base mutation at 367 bp of SEQ ID NO:1 in the sequence listing. Sheep with the TT and TC genotypes at this site have significantly higher weaning weights (at 60 days of age) than those with the CC genotype. Using Charolais sheep as the research subject, this invention utilizes PCR amplification of SNP variations in exon 14 of the sheep EGFR gene to analyze the effect of specific genotypes at this variation site on weaning weight in Charolais sheep. Based on this analysis, selective breeding of Charolais sheep can be conducted to improve weaning weight, providing marker resources for marker-assisted selection breeding of sheep growth traits.

This invention relates to the field of pig selective breeding technology, and particularly to a SNP molecular marker, primers, and applications related to the IFITM1 gene and inter-sex disease in pigs. The nucleotide sequence of this molecular marker is SEQ ID NO:1, and the 32nd base Y in the nucleotide sequence of the SNP molecular marker is a polymorphic site. At this polymorphic site, individuals with genotypes TT and TC exhibit inter-sex disease characteristics, while individuals with genotype CC do not exhibit inter-sex disease characteristics. The SNP molecular marker is located in the second exon of the IFITM1 gene on pig chromosome 2. This SNP molecular marker is closely related to the inter-sex disease trait in pigs. By detecting the SNP site using the primers disclosed in this invention, individuals carrying the pathogenic gene can be screened for use in breeding selection of pigs to prevent the spread of inter-sex diseases in pigs.

This invention relates to the field of pig selective breeding technology, and particularly to a SNP molecular marker, primers, and applications related to the NOBOX gene and intersex pigs. The nucleotide sequence of this SNP molecular marker is SEQ ID NO:1, and the 231st base S in the nucleotide sequence of the SNP molecular marker is a polymorphic site; at this polymorphic site, individuals with genotypes CC and CG exhibit abnormal sex development, while individuals with genotype GG exhibit normal sex development. The primers of this invention include F1 and a downstream primer R1. The SNP molecular marker of this invention is closely related to intersex disease traits in pigs and can be effectively used in breeding pigs to prevent abnormal sex development, achieving eugenic goals in pigs, and also providing a reference for human sex development abnormalities.

The present disclosure relates at least in part to genetic engineering of vegetable (e.g., tomato) plants to, for example, produce heme, a molecule contributing to a meat-like flavor, and to the incorporation of male sterility in these plants to facilitate the production of F1 hybrid seeds. Described herein, in some embodiments, are methods for constructing and introducing into such plants genetic constructs containing both a heme gene and a male sterility gene, the resulting plants, and the use of these plants in hybrid seed production. The present disclosure also provides at least in part methods for enhancing lycopene content in plants, such as tomato plants, either through genetic modification or selective breeding. While introducing key genes like Psy is effective, adding multiple genes or knocking out competing pathways can lead to more substantial increases in lycopene levels. Enhanced lycopene content can provide added health benefits, including potential cancer protection, making these plants, such as tomatoes, particularly valuable in the context of functional foods. The plants may be of varieties with high lycopene content combined with genetic modifications as provided herein. The present disclosure offers a comprehensive solution for producing plants (e.g., tomatoes) with increased nutritional value.

This invention provides a combination of SNP molecular markers for predicting the lysine content of sweet corn kernels and its application, belonging to the field of selective breeding technology for sweet corn. The SNP molecular marker combination provided by this invention consists of 20,000 SNP loci located on the maize B73 reference genome version v5. This combination can be used for marker-assisted breeding of high-quality protein (high lysine content) sweet corn, and also for aggregate breeding of multiple traits in sweet corn. The method for predicting the lysine content of sweet corn kernels provided by this invention only requires detecting the genotype of the breeding material to predict the content of high-quality protein (lysine) in the sweet corn kernels. It has the advantages of clear selection targets and being unaffected by environmental factors. Furthermore, the prediction of high-quality protein (lysine) content in the kernels provided by this invention is mainly performed at the sweet corn harvest period, enabling early detection.

The invention provides a gene mutant capable of overcoming distant hybridization lethal and a detection primer thereof. According to the gene mutant capable of overcoming distant hybridization lethal, an NtHL1S gene is mutated, and bases at the 472nd site and the 473rd site are replaced with A; the gene mutant directly participates in regulation and control, and the distant hybridization lethal phenomenon, especially the hybridization lethal phenomenon between common tobacco and African tobacco, is overcome by inhibiting expression of the NtHL1S gene in the tobacco for the first time. According to the method disclosed by the invention, the tobacco gene NtHL1S is mutated by utilizing a CRISPR / Cas9 method, and the situation that the hybridization lethal phenomenon between common tobacco and African tobacco disappears due to the mutation of the NtHL1S gene is found. A primer combination is developed aiming at an NtHL1S gene mutation site, and is used for transformation of a target site for gene identification, and rapid screening of a target single plant in molecular marker-assisted selective breeding offspring materials.