201 results about "Selective breeding" patented technology

The invention discloses a silver carp 20K liquid phase chip and application thereof, the liquid phase chip comprises a probe combination of 20,909 SNP molecular markers covering a whole genome, the physical positions of the 20,909 SNP molecular markers are determined based on sequence alignment of the silver carp genome (GCA041475455.1), and the site information of the 20,909 SNP molecular markers is shown in the specification table 1. The invention provides a silver carp first type whole genome SNP liquid phase breeding chip, which effectively reduces the application cost of silver carp in genetic diversity analysis, QTL positioning, GWAS analysis and the like in scientific research, solves the problem that no applicable product exists in silver carp molecular auxiliary breeding and whole genome selective breeding, accelerates the progress of silver carp basic research and breeding, and has a wide application prospect. The method is of great significance in improving the utilization efficiency of silver carp germplasm resources, accelerating the breeding process of excellent characters and improving the economic value and environmental adaptability of bred varieties.

The invention relates to the technical field of bioinformatics, in particular to a goat selective breeding method and system based on a genetic algorithm, and the method comprises the following steps: based on goat population genetic initialization data, extracting a character characteristic index and a genetic relationship parameter of each pairing combination in a population, analyzing genetic diversity, character matching degree and genetic relationship, and calculating the genetic diversity, character matching degree and genetic relationship; and screening matched individuals and pairing hereditary characteristics, and rearranging and recombining to obtain primary pairing data of goat breeding. According to the method, genetic diversity and character matching degree are analyzed, genetic diversity and excellent character inheritance are balanced, genetic resource utilization efficiency is improved, local optimal combinations are accurately screened, genetic drift risks are reduced, character stability is enhanced, a two-point crossing rule and global character dynamic analysis are combined, and global breeding layout is optimized; according to the method, short-term improvement and long-term stability are both considered, the genetic structure is optimized through Pareto rule screening and global state updating, and the sustainability and accuracy of a breeding scheme are improved.

The invention discloses a linked molecular marker of a major QTL (quantitative trait loci) uq2 of a peanut legume singulation character as well as an identification method and application of the linked molecular marker. The molecular marker and a major QTL site uq2 of the peanut legume singulation character are co-localized on a second chromosome of a peanut genome, and the molecular marker comprises molecular markers SNP4 and SNP57. The physical position of the molecular marker SNP4 is the 11591222th base position of the second chromosome of a cultivated peanut genome, and the polymorphism is C / T; the physical position of the molecular marker SNP57 is the 11894442 base position of the second chromosome of the cultivated peanut genome, and the polymorphism is A / T. The linked molecular marker is obtained by using a primer group. The nucleotide sequences of the primer group are respectively as shown in SEQ ID No. 1 to SEQ ID No. 6. The major QTL locus uq2 of the peanut legume constriction character as well as the linked molecular marker and the identification method of the major QTL locus uq2 are suitable for gene localization of the peanut legume constriction character and molecular marker-assisted selective breeding.

Provided are a dairy goat whole-genome 55K SNP chip and a use thereof. The chip has a total of 54344 SNP sites. The provided dairy goat whole-genome 55K SNP chip can be used for the identification and kinship analysis of imported dairy goat breeds and domestically bred dairy goat breeds, and can also be used in multiple fields such as dairy goat whole-genome association analysis, whole-genome selective breeding, population genetics analysis, and trait-related candidate gene and QTL site analysis and positioning, and has high value in both production applications and scientific research.

The invention belongs to the field of molecular biology, and discloses acquisition of a waterlogging-resistant major QTL qWT1.1 in a corn seedling stage and development and application of a molecular marker primer of the waterlogging-resistant major QTL qWT1.1. The invention provides a major QTL qWT1.1 for controlling the waterlogging survival rate in the corn seedling stage, the major QTL qWT1.1 is located at the fifth version physical position of a reference genome in the first staining of the corn, the fifth version physical position of the reference genome is Chr1: 25904794-27039204, the major QTL site is used for controlling waterlogging-resistant phenotypic variation, an excellent haplotype SNP marker closely linked with the major QTL site is located at the 27039148th basic group of a first chromosome of a reference genome of the corn B73, and the excellent haplotype SNP marker is located at the 27039148th basic group of a second chromosome of the reference genome of the corn B73. The explained waterlogging survival rate phenotypic contribution rate is 10.03%, the PARMS marker designed by the SNP is used for detecting a corn inbred line group, the operation is simple and convenient, the typing is clear, the good selection effect on characters such as root length, root number, plant height, branch number and yield under the stress of corn waterlogging is realized, and the application prospect is wide. The molecular marker can be used for molecular marker-assisted selective breeding of the waterlogging resistance character in the corn seedling stage.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to cotton fiber length and application thereof. The invention also discloses an SNP (Single Nucleotide Polymorphism) site related to the length of the cotton fiber, and the SNP site is positioned at the 95547325 site on the No. A05 chromosome in a cotton genome. Experiments prove that the length of the cotton fiber can be effectively identified by detecting the genotype of the SNP site based on the SNP molecular marker, and the identification method is simple to operate, contributes to identification and auxiliary selective breeding of the length of the cotton fiber, especially early identification and screening of the length of the cotton fiber, and has a good application prospect. The method is of great significance in breeding long-fiber cotton varieties and accelerating genetic improvement of cotton.

The invention discloses an SNP (Single Nucleotide Polymorphism) site related to the number of spikelet per ear and the number of grains per ear of wheat and application of the SNP site. The SNP site comprises a kit for identifying or assisting in identifying the number of spikelet per ear and the number of grains per ear of wheat, primers and application of the elements in identifying or assisting in identifying the number of spikelet per ear and the number of grains per ear of wheat. The invention provides a new method for molecular marker-assisted selective breeding of wheat, and has important significance in cultivation and research of high-yield wheat varieties.

The invention discloses application of a KASP molecular marker in identification of light yellow flower color traits of muskmelons, and belongs to the technical field of molecular markers. According to the KASP molecular marker for identifying the light yellow flower color character of the muskmelon, a genetic segregation population is constructed by taking a dark yellow flower color muskmelon and a light yellow flower color muskmelon as parents, and a stably inherited marker site chr04: 26634798G / A is obtained through molecular marker technology identification. The marker is closely related to the light yellow flower color character of the muskmelon, and has the characteristics of strong specificity and good stability. The molecular marker provided by the invention can realize rapid genotype detection of a muskmelon material through a high-throughput genotyping system, and the identification result is accurate and reliable and is not influenced by environmental factors. The molecular marker can be applied to molecular marker-assisted selective breeding of melon flower color characters, the breeding efficiency is remarkably improved, the breeding period is shortened, the breeding cost is reduced, and an effective molecular breeding tool is provided for directional improvement of melon flower colors.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker combination, a molecular probe combination, a liquid phase chip and a kit for corn genotyping and application of the SNP molecular marker combination, the molecular probe combination, the liquid phase chip and the kit, and belongs to the technical field of gene chips. The SNP molecular marker combination and the liquid chip provided by the invention can be used for efficient and rapid genetic typing of corn germplasm resources, and are low in cost and suitable for corn biological breeding. The SNP marker provided by the invention uniformly covers the whole corn reference genome, provides a high-quality molecular marker for silage corn whole genome selective breeding, and can also be used for map-based cloning of important agronomic trait genes of silage corn.

The invention relates to a pigeon whole genome 5K liquid phase chip and a manufacturing method and application thereof, and belongs to the technical field of molecular biology. According to the pigeon whole genome 5K liquid phase chip disclosed by the invention, the pigeon whole genome 5K liquid phase chip is composed of 5483 SNP molecular markers, and the positions of the SNP molecular markers on a pigeon reference genome Cliv1.0 are as shown in a table 1. Whole genome re-sequencing is carried out on local pigeon varieties and introduced pigeon varieties, quality control screening is carried out through the SNP locus screening strategy to obtain a core SNP locus set, functional SNP loci related to growth and reproductive performance are added, and the method has the advantages of being wide in genetic range, high in pertinence, low in detection cost and high in detection efficiency and has good application prospects. The method has the beneficial effects that the method has important significance on accurate identification and evaluation of pigeon germplasm resources and molecular marker-assisted selective breeding.

The invention belongs to the technical field of animal breeding, and particularly relates to a molecular marker closely linked with beef quality characters, a primer pair, a detection reagent and application. The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1, the nucleotide shown as SEQ ID NO.1 has G / A polymorphism at the 831th bp, when the genotype of the 831th site from the 5'end of the molecular marker is GG, the meat color brightness and intramuscular fat content of an individual are higher than those of a GA genotype individual, and the cooking loss of the individual is lower than that of the GA genotype individual, and the molecular marker can be used for selective breeding of high-quality beef cattle.

The invention discloses application of a KASP molecular marker in identification of sweet melon peel color traits, and belongs to the technical field of molecular markers. The invention aims to promote gene localization and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on sweet melon pericarp color characters. The KASP molecular marker is a KASP marker RW2; the KASP marker RW2 is designed according to an SNP (Single Nucleotide Polymorphism) site at the position of Chr04: 526653. According to the application, the KASP molecular marker RW2 is used for performing genetic typing on the red-peel muskmelon and the white-peel muskmelon, the DNA of a target plant can be identified only through one-time PCR amplification, the whole detection process is simple and convenient, and complex steps such as enzyme digestion, electrophoresis and sequencing are not needed. Through the high-throughput genotyping system, a genotyping graph and a genotyping value can be rapidly obtained, so that rapid identification of the genotype of a target plant is realized. The KASP molecular marker RW2 provided by the invention can be more efficiently applied to the breeding work of sweet melon peel color germplasm.

The invention discloses a molecular marker related to the oil content of brassica napus and application of the molecular marker, and belongs to the technical field of molecular marker assisted breeding. A transcription factor BnaA09.MYB52 for coding an MYB structural domain is proved to be capable of influencing the oil content of cabbage type rape seeds, molecular markers corresponding to different haplotypes of the gene and primers of the molecular markers are designed by utilizing re-sequencing data, and the DNA molecular markers can be used for distinguishing the functional haplotypes of the gene of a cabbage type rape strain to be detected; the gene molecular marker can be used for detecting and predicting the oil content of the brassica napus, can also be used for molecular marker-assisted selective breeding of the high-oil-content brassica napus, can effectively accelerate the process of high-oil-content breeding of the brassica napus, is suitable for large-scale popularization and application, and has a wide application prospect.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for identifying upland cotton fiber strength character and application thereof, and belongs to the technical field of plant molecular biology. The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for identifying upland cotton fiber strength character, which comprises two SNP molecular marker sites which are both positioned on an A10 chromosome of an upland cotton genome. The method has the characteristics of simplicity in operation, accurate result, convenience in repetition and the like, is suitable for screening upland cotton germplasm resources with different fiber strengths, identifies excellent sites with potential application values for molecular marker-assisted selective breeding, and has a wide application prospect.

The invention discloses a chip for genome breeding and variety identification of tilapia mossambica. The invention provides a group of SNP (Single Nucleotide Polymorphism) marker combination for tilapia mossambica, which comprises 50000 SNP markers which are respectively SNP1-SNP50000 markers. According to the invention, the SNP marker combination related to economic characters of tilapia mossambica is integrated, representative sites with uniform coverage are selected, a biological probe for site typing is developed, and the site coverage, typing accuracy and GS accuracy of the SNP marker combination are basically consistent with those of re-sequencing; the method can be applied to the aspects of tilapia germplasm resource identification, genetic relationship identification, SNP typing, variety identification, molecular breeding, DNA fingerprint database construction, variety purity detection, germplasm resource genetic analysis, whole genome selective breeding, functional gene positioning, genetic map construction, genetic evolution analysis, whole genome association analysis and the like.

The invention discloses a pea whole genome 20K SNP (Single Nucleotide Polymorphism) liquid phase breeding chip and application thereof, the liquid phase breeding chip comprises 21 and 659 SNP loci on a pea whole genome, the SNP loci comprise published 235 reliable functional loci obviously related to 57 characters, and examples for pea sample genetic typing are provided. The chip can be widely applied to important character analysis, germplasm resource genetic diversity analysis, variety identification analysis, auxiliary selective breeding, population structure analysis, heredity and evolution analysis, genetic relationship identification, whole genome association analysis or genome selection of peas, and is helpful for improving the pea breeding efficiency; the innovation and improvement of pea varieties are promoted.

The invention discloses an introduced-variety pig 10K SNP liquid chip based on targeted capture sequencing and application, and belongs to the technical field of biomolecular breeding. Target SNP sites are collected, selected and filtered, an SNP site set with comprehensive performance is screened out, a probe is designed on the basis of the SNP site set, the introduced variety pig 10K SNP liquid phase chip is further prepared, the SNP sites of the chip have the characteristics of uniformity, polymorphism, compatibility, stability and functionality, pig genomes can be covered to the maximum extent, and the detection sensitivity is high. Meanwhile, the selection of pig genomes can be greatly reduced, especially the cost of early selection of variety pigs is reduced, in addition, the selection efficiency and filling accuracy of chip sites filled to more than 50K can be ensured, and the requirements of subsequent variety identification, lineage analysis, genetic relationship identification, genome selective breeding and whole genome association analysis are met.

The invention relates to the technical field of biology, in particular to an SNP (Single Nucleotide Polymorphism) marker combination for identifying tilapia streptococcus agalactiae disease resistance and application thereof. The invention discloses 11 SNP loci significantly related to streptococcus agalactiae resistance in a tilapia UTRN gene, and constructs 18 SNP marker combinations for streptococcus agalactiae resistance phenotype identification, so that the resistance phenotype identification accuracy and hereditary stability can be effectively improved. Besides, according to the SNP typing primer and method based on the # imgabs0 # MALDI-TOF mass spectrum platform, high-throughput and high-precision genetic typing can be achieved, and the SNP typing primer and method have important application value in resistance phenotype identification and assistant selective breeding.

The invention discloses a corn selective breeding effect analysis method integrating population inheritance and deep learning, and the method comprises the steps: collecting corn germplasm resource data, including phenotype data, genotype data and environmental data; performing standardization processing and missing value filling on the phenotypic data, and calculating population genetic parameters including allele frequency, linkage imbalance and population structure stability; performing correlation analysis on the population genetic parameters and phenotypic data by using a deep learning model, predicting phenotypic values of candidate parents by using the trained model, calculating breeding indexes in combination with the population genetic parameters, and providing guidance for corn breeding decisions based on prediction results; through organic combination of a deep learning technology and a population genetics principle, accurate prediction and evaluation of the corn selective breeding effect are realized, the breeding efficiency is improved, and a new technical path is provided for corn variety improvement.

The invention provides a Sichuan local chicken 11K SNP liquid phase chip and application thereof, the liquid phase chip is prepared from a Sichuan local chicken 11K SNP molecular marker combination, the Sichuan local chicken 11K SNP molecular marker combination is composed of 11747 SNP molecular markers, and chromosome positions and numbers of the 11747 SNP molecular markers on a chicken reference genome GRCg7b are shown in a specification table 1. The chip is high in detection flux, high in target site detection rate (99.91%) and accurate and reliable in typing result (the repetition consistency rate is 99.82%), and is a molecular marker chip with low density, high accuracy and low cost; the method can be used for chicken germplasm resource identification and improvement, genetic diversity evaluation, population structure analysis, genetic relationship identification, transmission map construction and QTL positioning, whole genome association analysis, molecular marker-assisted selection, whole genome selective breeding and the like.

The invention belongs to the technical field of biology, and particularly relates to a barley grain weight related gene HvTGW1s and application thereof. The invention discloses a barley grain weight related gene HvTGW1s, the base sequence of the barley grain weight related gene HvTGW1s is shown as SEQ ID NO: 1, and the barley grain weight related gene HvTGW1s has the function of increasing the thousand grain weight, grain width and grain length of barley. The invention further discloses the KASP marker associated with the barley yield character and application of the KASP marker in barley yield assisted selective breeding. The invention further discloses a kit for assisting in identifying the barley yield. The method is applied to barley yield assisted selection breeding, the breeding efficiency can be effectively improved, and the breeding process is accelerated.

The invention belongs to the field of biotechnology application, and particularly relates to a goose whole genome liquid phase chip and application thereof. The SNP sites on the liquid phase chip are located in a goose reference genome GCA013030995.1, and the number of the SNP sites is 30000. The chip can specifically identify the genetic relationship between commercial geese and local geese, and can also be applied to whole genome association analysis, whole genome selective breeding, target trait QTL positioning analysis, population genetics analysis and the like. According to the invention, by capturing the reference gene and loci in the real sequence thereof, the coverage of sequencing data is improved, and the application value of the chip is enhanced.

The invention relates to the technical field of genomics, molecular biology, bioinformatics and whole-genome selective breeding, in particular to a whole-genome liquid chip for apostichopus japonicus and application of the whole-genome liquid chip. The liquid phase chip contains sequences of background SNP loci and functional SNP loci which are used for gene analysis and are positioned on an apostichopus japonicus reference genome; wherein the functional SNP sites are associated with important economic characters of the apostichopus japonicus. The invention also discloses application of the liquid chip in apostichopus japonicus genome selective breeding, important economic character gene positioning, genetic diversity analysis, germplasm resource improvement and protection.

The invention discloses a genetic typing chip for synergistically breeding fast-growing and high-temperature-resistant characters of procambarus clarkii and an application method of the genetic typing chip. Aiming at the technical bottlenecks that the traditional procambarus clarkia breeding period is long, the character selection efficiency is low and multi-character collaborative breeding is difficult to realize, a 50K cGPS liquid-phase breeding chip, namely 'Hailongcore No.1', is constructed by taking procambarus clarkia populations of different geographical populations as genetic variation sources; single nucleotide polymorphism molecular markers obviously associated with fast-growing and high-temperature-resistant characters of the procambarus clarkii are screened through whole genome association analysis, and a breeding model is constructed according to the single nucleotide polymorphism molecular markers and is applied to improvement of economic characters of the procambarus clarkii. According to the method, rapid typing of procambarus clarkii individuals can be achieved, the problems that traditional selective breeding is long in period, poor in accuracy, low in efficiency and free of theoretical basis are solved, an efficient molecular tool is provided for improved variety breeding of procambarus clarkii, the breeding efficiency of stress-resistant high-yield new varieties is remarkably improved, and the method has important industrial application value.

The invention provides a KASP marker of a soybean shade-tolerant gene Glyma.06g213100 and application of the KASP marker, the KASP marker is four SNP variation sites of the soybean shade-tolerant gene Glyma.06g213100, soySNP-1 is subjected to base T-A substitution at 21, 485 and 858 bp, soySNP-2 is subjected to base A-G substitution at 21, 486 and 271 bp, soySNP-3 is subjected to base A-G substitution at 21, 485 and 456 bp, and soySNP-4 is subjected to base T-G substitution at 21, 486 and 286 bp. The SNP and KASP markers provided by the invention can be used for molecular marker-assisted selective breeding of the soybean shade-tolerant character, and have important theoretical and practical guiding significance for accelerating the genetic improvement process of soybean shade-tolerant breeding and improving the breeding selection efficiency.

The invention discloses a molecular marker of a brassica napus salt-tolerant gene BnaA07.GRP3 and application of the molecular marker, and belongs to the technical field of rape breeding. According to the invention, two haplotypes hap0 and hap1 of the salt-tolerant gene BnaA07. GRP3 are found for the first time, a corresponding molecular marker is provided for the optimal haplotype hap1 of the BnaA07. GRP3, and the optimal haplotype hap1 of the BnaA07. GRP3 is specifically amplified, so that the salt-tolerant material of the brassica napus is identified. The salt-resistant material of the brassica napus can be quickly and accurately identified, and the repeatability is good. The method is applied to auxiliary selective breeding of salt-resistant characters of the brassica napus, the breeding efficiency of the salt-resistant brassica napus can be greatly improved, the selection range of salt-resistant materials is expanded, the method has huge application potential and prospects, and an ideal way is provided for cultivating the salt-resistant brassica napus.

The invention relates to the field of biomolecular detection, in particular to a KASP molecular marker related to wheat stripe rust resistance characters, a primer pair and application of the KASP molecular marker and the primer pair. According to the invention, 559 parts of common wheat germplasm resources are subjected to whole genome association analysis, a stable stripe rust resistance site QYr.hzau-3B. 1 is identified on a wheat 3B chromosome long arm, and a KASP molecular marker (KASP molecular marker KASP 3B2800) is developed aiming at the site and is used for screening large-scale materials. The KASP molecular marker provided by the invention provides a new site and a new marker for wheat stripe rust resistance breeding; meanwhile, the KASP molecular marker can be used for rapidly screening wheat germplasm containing the site, so that the time cost is effectively saved, and the progress of wheat resistance germplasm genetic background detection and wheat disease-resistant gene-assisted selective breeding is accelerated.

The invention belongs to the technical field of breeding, and particularly relates to a fine-wool sheep genome selective breeding method. According to the method, genetic typing is carried out on each sample by using a fine-wool sheep 50K SNP liquid phase chip, statistical analysis is carried out through genetic typing and phenotype data, RRBLUP and SSGBLUP are used for training population genotype data and phenotype data and calculating prediction accuracy, and finally, the net wool amount, wool length, wool fiber diameter and weight are selected as four breeding target characters. A genome breeding value (GEBV) of the weight, the wool length, the wool fiber diameter and the net wool amount of the fine-wool sheep is used as a denominator, the standard economic value of each character is used as a molecule, a fine-wool sheep genome comprehensive selection index estimation model (GPI) is formulated, and selective breeding of the fine-wool sheep genome is performed according to the GPI numerical value. A genome selection strategy with high accuracy and low cost is provided, and the method has important significance on high-quality development of fine-wool sheep breeding industry and industry.

The invention discloses a dCAPS molecular marker for detecting the moisture content and dehydration rate of corn kernels and application of the dCAPS molecular marker, and belongs to the technical field of corn molecular breeding. On the basis of correlation analysis of a gene ZmNAD, a significant SNP related to the moisture content and the dehydration rate of grains is identified, and then a dCAPS molecular marker ZmNAD-SNP1-Sac1 with the sequence as shown in SEQ ID NO.1 and a primer pair with the sequence as shown in SEQ ID NO.4-5 are developed. The dCAPS molecular marker and the primer pair thereof provided by the invention can be applied to identification of corn varieties with low moisture content and high dehydration rate of grains and molecular marker-assisted selective breeding, and can effectively save the detection cost, shorten the period and improve the detection efficiency. The invention provides a new technical means for screening corn materials with low water content and high dehydration rate of grains, and has a wide application prospect.

The invention relates to the technical field of pigs, in particular to a molecular marker related to pig carcass length and intramuscular fat content characters and application. The molecular marker is an SNP (Single Nucleotide Polymorphism) site of nucleotide at a position 7311198 of a chromosome 1 NC010443.5 of a pig reference genome GCF 000003025.6. The molecular marker has the advantages that the molecular marker can be applied to pig breeding; the molecular marker is closely associated with the characteristics of pig carcass length and intramuscular fat content. The molecular marker can be applied to molecular-assisted selective breeding of high-quality pig carcass and meat quality traits.