145 results about "Selective breeding" patented technology

The invention discloses a silver carp 20K liquid phase chip and application thereof, the liquid phase chip comprises a probe combination of 20,909 SNP molecular markers covering a whole genome, the physical positions of the 20,909 SNP molecular markers are determined based on sequence alignment of the silver carp genome (GCA041475455.1), and the site information of the 20,909 SNP molecular markers is shown in the specification table 1. The invention provides a silver carp first type whole genome SNP liquid phase breeding chip, which effectively reduces the application cost of silver carp in genetic diversity analysis, QTL positioning, GWAS analysis and the like in scientific research, solves the problem that no applicable product exists in silver carp molecular auxiliary breeding and whole genome selective breeding, accelerates the progress of silver carp basic research and breeding, and has a wide application prospect. The method is of great significance in improving the utilization efficiency of silver carp germplasm resources, accelerating the breeding process of excellent characters and improving the economic value and environmental adaptability of bred varieties.

The invention belongs to the field of molecular biology, and discloses acquisition of a waterlogging-resistant major QTL qWT1.1 in a corn seedling stage and development and application of a molecular marker primer of the waterlogging-resistant major QTL qWT1.1. The invention provides a major QTL qWT1.1 for controlling the waterlogging survival rate in the corn seedling stage, the major QTL qWT1.1 is located at the fifth version physical position of a reference genome in the first staining of the corn, the fifth version physical position of the reference genome is Chr1: 25904794-27039204, the major QTL site is used for controlling waterlogging-resistant phenotypic variation, an excellent haplotype SNP marker closely linked with the major QTL site is located at the 27039148th basic group of a first chromosome of a reference genome of the corn B73, and the excellent haplotype SNP marker is located at the 27039148th basic group of a second chromosome of the reference genome of the corn B73. The explained waterlogging survival rate phenotypic contribution rate is 10.03%, the PARMS marker designed by the SNP is used for detecting a corn inbred line group, the operation is simple and convenient, the typing is clear, the good selection effect on characters such as root length, root number, plant height, branch number and yield under the stress of corn waterlogging is realized, and the application prospect is wide. The molecular marker can be used for molecular marker-assisted selective breeding of the waterlogging resistance character in the corn seedling stage.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to cotton fiber length and application thereof. The invention also discloses an SNP (Single Nucleotide Polymorphism) site related to the length of the cotton fiber, and the SNP site is positioned at the 95547325 site on the No. A05 chromosome in a cotton genome. Experiments prove that the length of the cotton fiber can be effectively identified by detecting the genotype of the SNP site based on the SNP molecular marker, and the identification method is simple to operate, contributes to identification and auxiliary selective breeding of the length of the cotton fiber, especially early identification and screening of the length of the cotton fiber, and has a good application prospect. The method is of great significance in breeding long-fiber cotton varieties and accelerating genetic improvement of cotton.

The invention discloses an SNP (Single Nucleotide Polymorphism) site related to the number of spikelet per ear and the number of grains per ear of wheat and application of the SNP site. The SNP site comprises a kit for identifying or assisting in identifying the number of spikelet per ear and the number of grains per ear of wheat, primers and application of the elements in identifying or assisting in identifying the number of spikelet per ear and the number of grains per ear of wheat. The invention provides a new method for molecular marker-assisted selective breeding of wheat, and has important significance in cultivation and research of high-yield wheat varieties.

The invention discloses application of a KASP molecular marker in identification of light yellow flower color traits of muskmelons, and belongs to the technical field of molecular markers. According to the KASP molecular marker for identifying the light yellow flower color character of the muskmelon, a genetic segregation population is constructed by taking a dark yellow flower color muskmelon and a light yellow flower color muskmelon as parents, and a stably inherited marker site chr04: 26634798G / A is obtained through molecular marker technology identification. The marker is closely related to the light yellow flower color character of the muskmelon, and has the characteristics of strong specificity and good stability. The molecular marker provided by the invention can realize rapid genotype detection of a muskmelon material through a high-throughput genotyping system, and the identification result is accurate and reliable and is not influenced by environmental factors. The molecular marker can be applied to molecular marker-assisted selective breeding of melon flower color characters, the breeding efficiency is remarkably improved, the breeding period is shortened, the breeding cost is reduced, and an effective molecular breeding tool is provided for directional improvement of melon flower colors.

The invention relates to a pigeon whole genome 5K liquid phase chip and a manufacturing method and application thereof, and belongs to the technical field of molecular biology. According to the pigeon whole genome 5K liquid phase chip disclosed by the invention, the pigeon whole genome 5K liquid phase chip is composed of 5483 SNP molecular markers, and the positions of the SNP molecular markers on a pigeon reference genome Cliv1.0 are as shown in a table 1. Whole genome re-sequencing is carried out on local pigeon varieties and introduced pigeon varieties, quality control screening is carried out through the SNP locus screening strategy to obtain a core SNP locus set, functional SNP loci related to growth and reproductive performance are added, and the method has the advantages of being wide in genetic range, high in pertinence, low in detection cost and high in detection efficiency and has good application prospects. The method has the beneficial effects that the method has important significance on accurate identification and evaluation of pigeon germplasm resources and molecular marker-assisted selective breeding.

The invention belongs to the technical field of animal breeding, and particularly relates to a molecular marker closely linked with beef quality characters, a primer pair, a detection reagent and application. The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1, the nucleotide shown as SEQ ID NO.1 has G / A polymorphism at the 831th bp, when the genotype of the 831th site from the 5'end of the molecular marker is GG, the meat color brightness and intramuscular fat content of an individual are higher than those of a GA genotype individual, and the cooking loss of the individual is lower than that of the GA genotype individual, and the molecular marker can be used for selective breeding of high-quality beef cattle.

The invention discloses application of a KASP molecular marker in identification of sweet melon peel color traits, and belongs to the technical field of molecular markers. The invention aims to promote gene localization and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on sweet melon pericarp color characters. The KASP molecular marker is a KASP marker RW2; the KASP marker RW2 is designed according to an SNP (Single Nucleotide Polymorphism) site at the position of Chr04: 526653. According to the application, the KASP molecular marker RW2 is used for performing genetic typing on the red-peel muskmelon and the white-peel muskmelon, the DNA of a target plant can be identified only through one-time PCR amplification, the whole detection process is simple and convenient, and complex steps such as enzyme digestion, electrophoresis and sequencing are not needed. Through the high-throughput genotyping system, a genotyping graph and a genotyping value can be rapidly obtained, so that rapid identification of the genotype of a target plant is realized. The KASP molecular marker RW2 provided by the invention can be more efficiently applied to the breeding work of sweet melon peel color germplasm.

The invention discloses a chip for genome breeding and variety identification of tilapia mossambica. The invention provides a group of SNP (Single Nucleotide Polymorphism) marker combination for tilapia mossambica, which comprises 50000 SNP markers which are respectively SNP1-SNP50000 markers. According to the invention, the SNP marker combination related to economic characters of tilapia mossambica is integrated, representative sites with uniform coverage are selected, a biological probe for site typing is developed, and the site coverage, typing accuracy and GS accuracy of the SNP marker combination are basically consistent with those of re-sequencing; the method can be applied to the aspects of tilapia germplasm resource identification, genetic relationship identification, SNP typing, variety identification, molecular breeding, DNA fingerprint database construction, variety purity detection, germplasm resource genetic analysis, whole genome selective breeding, functional gene positioning, genetic map construction, genetic evolution analysis, whole genome association analysis and the like.

The invention relates to the technical field of biology, in particular to an SNP (Single Nucleotide Polymorphism) marker combination for identifying tilapia streptococcus agalactiae disease resistance and application thereof. The invention discloses 11 SNP loci significantly related to streptococcus agalactiae resistance in a tilapia UTRN gene, and constructs 18 SNP marker combinations for streptococcus agalactiae resistance phenotype identification, so that the resistance phenotype identification accuracy and hereditary stability can be effectively improved. Besides, according to the SNP typing primer and method based on the # imgabs0 # MALDI-TOF mass spectrum platform, high-throughput and high-precision genetic typing can be achieved, and the SNP typing primer and method have important application value in resistance phenotype identification and assistant selective breeding.

The invention discloses a corn selective breeding effect analysis method integrating population inheritance and deep learning, and the method comprises the steps: collecting corn germplasm resource data, including phenotype data, genotype data and environmental data; performing standardization processing and missing value filling on the phenotypic data, and calculating population genetic parameters including allele frequency, linkage imbalance and population structure stability; performing correlation analysis on the population genetic parameters and phenotypic data by using a deep learning model, predicting phenotypic values of candidate parents by using the trained model, calculating breeding indexes in combination with the population genetic parameters, and providing guidance for corn breeding decisions based on prediction results; through organic combination of a deep learning technology and a population genetics principle, accurate prediction and evaluation of the corn selective breeding effect are realized, the breeding efficiency is improved, and a new technical path is provided for corn variety improvement.

The invention relates to the technical field of genomics, molecular biology, bioinformatics and whole-genome selective breeding, in particular to a whole-genome liquid chip for apostichopus japonicus and application of the whole-genome liquid chip. The liquid phase chip contains sequences of background SNP loci and functional SNP loci which are used for gene analysis and are positioned on an apostichopus japonicus reference genome; wherein the functional SNP sites are associated with important economic characters of the apostichopus japonicus. The invention also discloses application of the liquid chip in apostichopus japonicus genome selective breeding, important economic character gene positioning, genetic diversity analysis, germplasm resource improvement and protection.

The invention discloses a genetic typing chip for synergistically breeding fast-growing and high-temperature-resistant characters of procambarus clarkii and an application method of the genetic typing chip. Aiming at the technical bottlenecks that the traditional procambarus clarkia breeding period is long, the character selection efficiency is low and multi-character collaborative breeding is difficult to realize, a 50K cGPS liquid-phase breeding chip, namely 'Hailongcore No.1', is constructed by taking procambarus clarkia populations of different geographical populations as genetic variation sources; single nucleotide polymorphism molecular markers obviously associated with fast-growing and high-temperature-resistant characters of the procambarus clarkii are screened through whole genome association analysis, and a breeding model is constructed according to the single nucleotide polymorphism molecular markers and is applied to improvement of economic characters of the procambarus clarkii. According to the method, rapid typing of procambarus clarkii individuals can be achieved, the problems that traditional selective breeding is long in period, poor in accuracy, low in efficiency and free of theoretical basis are solved, an efficient molecular tool is provided for improved variety breeding of procambarus clarkii, the breeding efficiency of stress-resistant high-yield new varieties is remarkably improved, and the method has important industrial application value.

The invention provides a KASP marker of a soybean shade-tolerant gene Glyma.06g213100 and application of the KASP marker, the KASP marker is four SNP variation sites of the soybean shade-tolerant gene Glyma.06g213100, soySNP-1 is subjected to base T-A substitution at 21, 485 and 858 bp, soySNP-2 is subjected to base A-G substitution at 21, 486 and 271 bp, soySNP-3 is subjected to base A-G substitution at 21, 485 and 456 bp, and soySNP-4 is subjected to base T-G substitution at 21, 486 and 286 bp. The SNP and KASP markers provided by the invention can be used for molecular marker-assisted selective breeding of the soybean shade-tolerant character, and have important theoretical and practical guiding significance for accelerating the genetic improvement process of soybean shade-tolerant breeding and improving the breeding selection efficiency.

The invention discloses a molecular marker of a brassica napus salt-tolerant gene BnaA07.GRP3 and application of the molecular marker, and belongs to the technical field of rape breeding. According to the invention, two haplotypes hap0 and hap1 of the salt-tolerant gene BnaA07. GRP3 are found for the first time, a corresponding molecular marker is provided for the optimal haplotype hap1 of the BnaA07. GRP3, and the optimal haplotype hap1 of the BnaA07. GRP3 is specifically amplified, so that the salt-tolerant material of the brassica napus is identified. The salt-resistant material of the brassica napus can be quickly and accurately identified, and the repeatability is good. The method is applied to auxiliary selective breeding of salt-resistant characters of the brassica napus, the breeding efficiency of the salt-resistant brassica napus can be greatly improved, the selection range of salt-resistant materials is expanded, the method has huge application potential and prospects, and an ideal way is provided for cultivating the salt-resistant brassica napus.

The invention discloses a dCAPS molecular marker for detecting the moisture content and dehydration rate of corn kernels and application of the dCAPS molecular marker, and belongs to the technical field of corn molecular breeding. On the basis of correlation analysis of a gene ZmNAD, a significant SNP related to the moisture content and the dehydration rate of grains is identified, and then a dCAPS molecular marker ZmNAD-SNP1-Sac1 with the sequence as shown in SEQ ID NO.1 and a primer pair with the sequence as shown in SEQ ID NO.4-5 are developed. The dCAPS molecular marker and the primer pair thereof provided by the invention can be applied to identification of corn varieties with low moisture content and high dehydration rate of grains and molecular marker-assisted selective breeding, and can effectively save the detection cost, shorten the period and improve the detection efficiency. The invention provides a new technical means for screening corn materials with low water content and high dehydration rate of grains, and has a wide application prospect.

The invention relates to the technical field of pigs, in particular to a molecular marker related to pig carcass length and intramuscular fat content characters and application. The molecular marker is an SNP (Single Nucleotide Polymorphism) site of nucleotide at a position 7311198 of a chromosome 1 NC010443.5 of a pig reference genome GCF 000003025.6. The molecular marker has the advantages that the molecular marker can be applied to pig breeding; the molecular marker is closely associated with the characteristics of pig carcass length and intramuscular fat content. The molecular marker can be applied to molecular-assisted selective breeding of high-quality pig carcass and meat quality traits.

The invention discloses a KASP molecular marker primer combination for identifying soybean hundred-grain weight character and application of the KASP molecular marker primer combination, and belongs to the technical field of soybean agronomic character molecular marker development and molecular marker assisted breeding. The invention discloses a KASP molecular marker primer combination for identifying the soybean hundred-grain weight character. The sequence of the KASP molecular marker primer combination is shown as SEQ ID NO.3-5. The invention provides a novel and simple molecular marker and an auxiliary selection method through the obtained KASP marker for regulating the hundred-grain weight character gene of the soybean, and is suitable for rapid screening of the relative height of the hundred-grain weight of the soybean and molecular marker auxiliary breeding. The molecular marker can detect genotypes of materials with different hundred-grain weights in natural populations, can also be used for molecular-assisted selective breeding of soybean materials with different hundred-grain weights, replaces large-scale phenotypic screening, reduces field workload, and greatly improves screening accuracy and efficiency.

The invention belongs to the technical field of molecular biology, bioinformatics, sorghum genetics and whole-genome gene chips, and particularly relates to a sorghum whole-genome SNP molecular marker combination developed based on a targeted sequencing technology, a 10K liquid chip and application of the sorghum whole-genome SNP molecular marker combination and the 10K liquid chip. The SNP molecular marker combination comprises 10,000 SNP loci, and the specific information of the 10,000 SNP loci is as shown in a table 1; the physical positions of the 10000 SNP loci are determined by performing sequence alignment based on a reference genome of the wine red tassel sorghum variety HYZ-T2T with the version number of v1.0. A liquid chip developed based on the SNP molecular marker combination provided by the invention can be effectively applied to genetic diversity analysis, whole genome association analysis, genetic typing, molecular marker-assisted selective breeding, whole genome selective breeding and other applications of sorghum germplasm resources.

The invention discloses a molecular specific marker for identifying genetic sex of culter alburnus and application of the molecular specific marker, and relates to the technical field of animal molecular genetics. The specific preparation method comprises the following steps: firstly, extracting culter alburnus genome DNA; then screening by utilizing the genomic DNA of the culter alburnus to obtain a culter alburnus genetic sex molecule specific marker; the method comprises the following steps: designing a primer according to a culter alburnus genetic sex molecule specific marker, and amplifying a culter alburnus genome sample to be detected; if the PCR amplification product is a single bright specific band, the individual is a female individual, and if the PCR amplification product has two bands, the individual is a male individual. The genetic sex of the culter alburnus can be quickly and accurately identified by utilizing the method, so that the method has important scientific value on the research on the sex mechanism of the culter alburnus, and has important significance and application value on the sex control and selective breeding of the culter alburnus.

The invention provides an InDel molecular marker sequence related to wheat drought resistance as well as a detection method and application thereof, and belongs to the technical field of molecular markers. The invention provides a polymorphic site for forming different drought resistance of wheat, the polymorphic site only has two haplotypes: a sequence of a 724bp long fragment as shown in SEQ ID No.2 is inserted between the fragments 245-246bp as shown in SEQ ID No.1, and the haplotype is homozygous as a haplotype A; the drought resistance of to-be-detected wheat which is not inserted with the 724bp long fragment sequence shown at the site, is haplotype B homozygous and is determined to be haplotype A homozygous is higher than that of a variety determined to be haplotype B homozygous, so that the drought resistance of the to-be-detected wheat is determined to be haplotype A homozygous by detecting the condition of the polymorphic site when the wheat is subjected to molecular marker-assisted selective breeding, and the drought resistance of the to-be-detected wheat is determined to be haplotype A homozygous. The wheat with relatively high drought resistance can be found.

The invention relates to the technical field of gene chips, in particular to a masson pine SNP molecular marker combination and application thereof. The SNP molecular marker combination is composed of 113 SNP molecular markers and 709 SNP molecular markers, wherein the physical positions of the SNP molecular markers are determined by carrying out sequence alignment on the basis of a reference genome Pinus.tabuliformes V1.0 of the Chinese pines. According to the embodiment of the invention, the masson pine whole genome chip containing 113 and 709 SNP sites is provided for the first time, and has the advantages of high efficiency, low cost, good hereditary stability and the like; the method can be widely applied to different application scenes such as pinus massoniana germplasm resource identification, breeding material genetic background analysis, gene localization, whole-genome association analysis, whole-genome selective breeding and intelligent design breeding.

The invention relates to the technical field of molecular biology, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker for sheep horn length regulation and control and application thereof. The SNP molecular marker is characterized in that T / C mutation exists at the site 35071069 of the No.17 chromosome of a sheep reference genome AR-UIRambv2.0, and T / C mutation exists at the site 35071069 of the No.17 chromosome of the sheep reference genome. The sheep horn length can be identified by using the SNP molecular marker provided by the invention, and breeding can be selectively carried out according to environmental adaptability.

The present invention provides an application of a SNP genetic marker affecting chicken breast width in chicken genetic breeding, belonging to the fields of livestock and poultry genomic selective breeding and biotechnology. The SNP genetic marker affecting chicken breast width includes CB_tag11, which corresponds to the physical position 17298459 of chromosome 11 of the chicken reference genome bGalGal 1.mat.broi ler.GRCg7b version sequence information published by NCBI, and belongs to the 14th intron of the gene GSE1, where the base is A or G. CB_tag11 helps to genetically increase breast width. Its application in chicken genetic breeding is beneficial for improving breast width in laying hens and obtaining high-quality laying hen varieties.

The invention relates to the technical field of plant molecular genetic breeding, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker closely linked with white gourd pulp malic acid major QTL (Quantitative Trait Loci) and application thereof. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO.4, and G / A mutation exists at the 26th basic group of the sequence. The molecular marker and the white gourd pulp malic acid have a close linkage marker characteristic, so that a KSAP primer pair is developed on the basis of the SNP molecular marker. Results of the specific embodiment of the invention show that the KASP molecular marker provided by the invention can be used for identifying the content of malic acid in pulp of wax gourd seeds or early-stage seedlings with true leaves, and the method is high in accuracy, low in cost and short in consumed time. Therefore, the SNP molecular marker and the KASP primer pair provided by the invention can be used for molecular marker-assisted selective breeding of white gourds, and the process of white gourd flavor, taste and quality breeding is accelerated.

The invention discloses a pig whole genome low-density 6K SNP (Single Nucleotide Polymorphism) chip, and the chip contains 5,866 SNP loci. The SNP chip provided by the invention is suitable for a plurality of fields of pig whole genome association analysis, whole genome selective breeding, population genetics analysis, character-related candidate gene and QTL site analysis and positioning and the like, and has relatively high value in production application and scientific research.

The invention relates to an SNP (Single Nucleotide Polymorphism) molecular marker associated with the fatness of turbots fed with fermented soybean meal and application of the SNP molecular marker, and belongs to the technical field of molecular markers. The SNP molecular marker obviously associated with the fatness character is obtained by analyzing the fatness character of the turbots through genome-wide association analysis and comprises at least one of six SNP loci; the scophthalmus maximus molecular marker can be applied to scophthalmus maximus molecular assisted breeding, screening is conducted on the genome level through the breeding method, the breeding period can be greatly shortened, dependence on phenotypic characters is reduced, the selective breeding efficiency is improved, and the scophthalmus maximus molecular marker has good application prospects.

The invention discloses application of an SNP (Single Nucleotide Polymorphism) marker in an SUSD1 gene influencing the lambing number of goats. The marker is located on an SUSD1 gene of a goat chromosome 8, the specific SNP marker is T / C base mutation at the 336bp position of SEQ ID NO: 1 in a sequence table, and the lambing number of TT genotype and CT genotype individuals is remarkably higher than that of CC genotype individuals. According to the present invention, the Musheng black goats are adopted as the research object, the DNA sequence of the 14th exon of the goat SUSD1 gene is subjected to PCR amplification, the effect of the specific genotype of the variation site on the Musheng black goat lambing number is analyzed, and the Musheng black goat individual breeding is selected according to the analysis, such that the Musheng black goat lambing number can be increased, and the Musheng black goat lambing number can be improved. The breeding of a new variety (strain) taking the Muscheng black goat as a breeding material is accelerated, and a marker resource is provided for marker-assisted selective breeding of goat lambing number traits.

The present invention discloses a method for identifying the phenotype of turbot with low-salt tolerance, belonging to the field of selective breeding of economic traits of aquatic animals. The method is as follows: Prepare a culture pond, inject fresh water, and adjust the water temperature, dissolved oxygen to be consistent with the environment where the test population is located. After the test turbot population has fasted for 72 hours, place it in the fresh water pond for cultivation, and record the initial time. Check the color change of the pigmented side of the fish body every hour. After black stripes appear, record the appearance time of the black stripes. Immediately return the experimental fish to the normal salinity environment after the recording is completed, and the body color of the fish will recover. The determination of the phenotype of turbot with high-efficiency low-salt tolerance is realized according to the formula. The individual low-salt tolerance breeding phenotype values are arranged from large to small, and the individuals with the top rankings are selected. The phenotype identification method provided by the present invention can achieve more efficient and accurate results, and cause no damage to the tested individuals, laying a foundation for subsequent genetic evaluation and selective breeding of this trait.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker combination related to a corn plant height character, a gene chip and application of the SNP molecular marker combination and the gene chip, and relates to the technical field of plant molecular breeding, the SNP molecular marker combination comprises 21 SNP molecular markers, and the 21 SNP molecular markers are shown as SNP1-SNP21. The developed SNP molecular marker combination can be used for accurately identifying the plant height genotype of corn germplasm resources, and a novel tool is provided for molecular marker-assisted selective breeding. The molecular marker is used for identifying the genotype of the corn dwarf related site to judge the phenotype of the corn plant height, which is more efficient and more direct. Meanwhile, the screened haplotype marker of the plant height phenotype can be used for further development of a corn plant height function related site chip.