814 results about "Selective breeding" patented technology

The present invention discloses compositions and methodologies for the utilization of probiotic organisms in therapeutic compositions. More specifically, the present invention relates to the utilization of one or more species or strains of lactic acid-producing bacteria, preferably strains of Bacillus coagulans, for the control of gastrointestinal tract pathogens, including antibiotic-resistant gastrointestinal tract pathogens, and their associated diseases by both a reduction in the rate of colonization and the severity of the deleterious physiological effects of the colonization of the antibiotic-resistant pathogen. In addition, the present invention relates to the utilization of therapeutic compounds comprised of lactic acid—producing bacteria and anti-microbial agents such as antibiotics, anti-fungal compounds, anti-yeast compounds, or anti-viral compounds. The present invention also discloses methodologies for: (i) the selective breeding and isolation of probiotic, lactic acid-producing bacterial strains which possess resistance or markedly decreased sensitivity to anti-microbial agents (e.g., antibiotics, anti-fungal agents, anti-yeast agents, and anti-viral agents); and (ii) treating or preventing bacteria-mediated infections of the gastrointestinal tract by use of the aforementioned probiotic bacterial strains with or without the concomitant administration of antibiotics. While the primary focus is on the treatment of gastrointestinal tract infections, the therapeutic compositions of the present invention may also be administered to buccal, vaginal, optic, and like physiological locations.

The invention discloses a rice blast resistance gene Pi9 gene specificity molecular marker Pi9SNP as well as preparation and application thereof. The molecular marker Pi9SNP is prepared by amplifying from total DNA (Deoxyribonucleic Acid) of a rice blast resistant variety with rice blast resistance gene Pi9 by using a primer pair F1 and R1 so as to obtain a nucleotide segment I, and carrying out digestion on the nucleotide segment I by using restriction enzyme Hind III, so as to obtain a nucleotide segment II which is in specificity banding pattern with the obtained rice blast resistance gene Pi9. The molecular marker is the first one which is developed for marking Pi9 gene specificity SNP (Signal Nucleotide Polymorphism) for an internal sequence of the Pig gene, and has the following advantages that the specificity is high, the cost is low and the flux is high in practical application, and the molecular marker is widely applied to colonies of different genetic backgrounds. By utilizing the molecular marker, the efficiency in auxiliary molecular marker selective breeding, gene pyramiding breeding and transgenosis breeding is improved.

The invention discloses an alkaline pectinase production method and application in papermaking pulping. The alkaline pectinase production method comprises the steps of: selective breeding of a bacterial strain, culturing of the bacterial strain, fermentation through shaking a flask, and preparation of an enzyme liquid. The method is characterized in that the bacterial strain subjected to the selective breeding is achieved through separated screening of a naturally acquired bacterial sample and mutagenesis of the bacterial strain at cellular level, i.e. heat-resistant Bacillussubtillis with a laboratory number of MAPLE61 and preservation number of CCTCC NO:M2010004; and when the heat-resistant Bacillussubtillis is used for papermaking pulping, raw materials are soaked with an alkaline pectinase solution after the pickling treatment. When the invention is used for papermaking pulping, the prepared chemical pulp has favorable strength property and bleaching property; and after simple oxidation chlorine bleaching, the whiteness of the pump can reach 80-90 percent, breaking length can reach 6000-7000m, and meanwhile, alkali consumption is reduced about 20-40 percent and the production cost is reduced about 20 percent.

The invention discloses a gene-specific molecular marker Pi2SNP of a rice blast-resistant gene Pi2 as well as a preparation method and application thereof. The molecular marker Pi2SNP is a nucleotide fragment II in a specific banding pattern with the rice blast-resistant gene Pi2 obtained by amplifying F1 and R1 from the total DNA (Deoxyribose Nucleic Acid) of the rice blast-resistant variety carrying rice blast-resistant gene Pi2 by use of a primer to obtain a nucleotide fragment I and then performing enzyme digestion of the nucleotide fragment I by use of restriction endonuclease Pst I or Hinf I. The molecular marker is the first Pi2 gene-specific SNP marker developed for the sequence in the Pi2 gene, and has the advantages of high specificity and low cost and high flux in practical application, and can be widely applied to the colonies with different inheritance backgrounds. By adopting the molecular marker, the utilization efficiency of the gene in molecular marker-assisted selective breeding, gene pyramiding breeding and transgenic breeding can be improved.

The present invention relates to a bovine VDJ cassette (BF1H1) that provides the novel ability to develop chimeric immunoglobulin molecule capable of incorporating both linear T cell epitope(s) (CDR1H and CDR2H) as well as conformational B cell epitope(s) (exceptionally long CDR3H). Further, multiple epitopes can be incorporated for development of multivalent vaccine by replacing at least a portion of an immunoglobulin molecule with the desired epitope such that functional ability of both epitope(s) and parent VDJ rearrangement is retained. The antigenized immunoglobulin incorporating both T and B epitopes of interest is especially useful for development of oral vaccines for use in humans apart from other species including cattle. The long CDR3H in BF1H1 VDJ rearrangement originates from long germline D-genes. The novel bovine germline D-genes provide unique molecular genetic marker for sustaining the D-gene pool in cattle essential for immunocompetence via selective breeding. D-gene specific DNA probe permits typing and selection of breeding cattle stock for maximum gemline D gene pool for better health and disease prevention. The bovine D-genes are unique to cattle and, therefore, provide sensitive and specific forensic analytical tool using molecular biology techniques to determine tissues suspected of bovine origin.

The invention discloses a method for breeding a new variety of Taihang black goats. In the breeding method, a three way cross among a Taihang black goat, a Jintang black goat and a Nubian is performed, and individual bulk selection is used for selective breeding and propagation to breed the new variety of Taihang black goats. In the breeding method of the invention, by introducing the advantageous genes associated with the large body size, high yield and high milk productivity of the Jintang black goats and Nubian goats are introduced, the bred new variety of the Taihang black goats, apart from the characteristics of high adaptively and complete black hair of the Taihang black goat, has the advantages of large body size, high growing speed, high meat production capacity, high milk productivity and the like. Compared with Taihang black goats, the new variety has the advantages that: the lambing percentage is as high as over 150 percent; the growing speed is high; the body size is so large as the weight of a mature male goat is over 55 kilograms and the weight of a female goat is over 40 kilograms; the meal productivity is high; the meat quality is high; the milk yield is high; and the adaptability is high. Thus, the method is suitable for drylot feeding and market lamb production development.

The invention relates to a poultry breeding technology, in particular to a cultivation method of high-yield native laying chicken. The cultivation method comprises the following steps of: F-series cultivation, backcrossing royal chicken with the synthesis series of parental royal chicken and parental Loman laying chicken, obtaining an underlying group strain containing 75 percent of royal chicken consanguinity, and then carrying out continuous four-generation selective breeding; M-strain breeding cultivation: carrying out cross breeding on the parental royal chicken and the parental Loman laying chicken to obtain an underlying group strain containing 50 percent of the royal chicken consanguinity, and then carrying out continuous four-generation selective breeding; producing commercial generation in matching strains: by taking F strains as parental generation male parent and M strains as parental generation female parent, carrying out sex determination on filial generation by adopting an anal swelling method, wherein the female chicken are commercial-generation laying chicken. The cultivation method can cultivate new species (matching strains) of native laying chicken with high egg yield and excellent egg quality, the egg yield of one commercial chicken after entering into a pheasantry for 72 weeks reaches more than 235 and is improved by 50 percent compared with the egg yield of pure native laying chicken; and the commercial eggs have same appearance quality, protein quality and flavor characteristics with native eggs.

The invention discloses a method for breeding a new species of lean meat type pigs-Shennong No. 1, which includes: A) the erhualian sow as female parent and the yorkshire boar as male parent are in cross breeding to obtain the yorkshire*erhualian cross bred sow; then, cross breeding female parent is in cross breeding with the danish landrace boar to obtain danish*( yorkshire*erhualian) ternary hybrid pigs; B) a fundamental selective breeding group of danish*( yorkshire*erhualian) ternary hybrid pig is formed and selected as zero-generation of Shennong No. 1; C) the boars and sows are mated to obtain piglets, good piglets are selected gradually for seed reservation; D) the good piglets are continuously cultivated until sexual maturity, the outstanding individual female and male pig are reserved as the next generation of swine respectively according to reproductive performance and meat quality as well as carcass performance and growth performance; E) the step C) and D) are repeatedly operated for 4-15 times. The main selective breeding indexes of the Shennong No. 1 pig are based on the reproductive performance and meat quality, the Shennong No. 1 pigs have the advantages of good meat quality, which is manifested in red meat color, good water holding capacity, high intramuscular fat, and good tenderness and the like. Referring to the reproductive performance, the number of the first-born Shennong No. 1 sow is more than ten.

The invention discloses a selective breeding method for improving meat performance of black goats of Guizhou. The selective breeding method includes the steps of: A, utilizing black goats of Guizhou as female parents, and utilizing adult black goats of Lezhi and adult goats of Boer as male parents; B, hybridizing the adult goats of Boer as the male parents with breeding ewes selected and remained in a generation F1 in an artificial insemination manner, and selecting and remaining breeding ewes from obtained lambs in generation F2 according to the same selecting and remaining method used for the generation F1; C, hybridizing other high-quality black goats of Lezhi as the male parents with the breeding ewes selected and remained in the generation F2 in an artificial insemination manner, and selecting and remaining breeding ewes from obtained lambs in generation F3 according to the same selecting and remaining method used for the generation F1; and D, subjecting breeding rams selected and remained in the generations F1-F3 and breeding ewes selected and remained in the generation F3 in an artificial insemination manner to intersect-breeding. By the selective breeding method, weight of six-month and one-year-old black goats of Guizhou is remarkably increased, and original dressing percentage of the one-year-old ewes is increased from 47.4% to 49.2%.

The invention discloses a method for determining optimal SNP quantity as well as performing genome selective breeding on production performance of large yellow croakers through selection markers. The method comprises the following steps: performing phenotype determination and genomic sequencing on the production performance of individuals of a reference group to obtain SNP locus; screening out the qualified SNP locus and supplementing the deleted genotype; dividing the reference group into a training set and a validation set to perform hybridization validation; screening the SNP locus most remarkably associated with the character through single marker analysis, and then calculating GEBV of individuals of the validation set only by using the locus and through a GBLUP method; further obtaining breeding value estimation accuracy under each screening SNP quantity; finally determining the optimal quantity of SNP screening; and according to the optimal quantity, calculating the GEBV by the GBLUP method, further obtaining the breeding value estimation accuracy and performing genome selective breeding according to the value size. By the method, the genomic selection cost on the production performance of the large yellow croakers can be reduced remarkably.

The invention provides an SNP marker related to the sugar tolerance of grass carps and application of the SNP marker. The SNP marker comprises two SNP loci, namely H3 and H4, wherein H3 is located at position 1817 from the 5' end of the nucleotide sequence shown as SEQ ID NO:1 in the specification, and base at the position 1817 is T or C; H4 is located at position 1818 from the 5' end of the nucleotide sequence shown as SEQ ID NO:1 in the specification, and base at the position 1818 is G or T. The SNP marker provided by the invention is closely related to the sugar tolerance of the grass carps, and can be effectively used for identification or selective breeding of high-sugar-tolerance grass carp species.

The invention provides a breeding method for the germ plasm of a new translocation line wheat-rye T2BL.1RS, which is characterized in that, distant hybridization is performed by taking common wheat variety Xiaoyan 6 as female parent and relative plant rye variety German White as male parent; seeds are acquired after immature embryo salvation culture, colchicine half root soaking method and chromosome doubling; backcross seeds are acquired after disease resistance identification, cytological identification and backcrossing utilizing the original parent Xiaoyan 6 as male parent; then after disease resistance identification and cytological identification, individual plants with excellent comprehensive characters are selected in direction for self crossing, and after continuous self cross selective breeding for six generations, the finally acquired individual plant is confirmed to be wheat-rye T2BL.1RS translocation line after identified by continuous total genome in situ hybridization and three-probe multicolor fluorescence in situ hybridization. The breeding method for the germ plasm of a new translocation line wheat-rye T2BL.1RS has the advantages that, the wheat-rye T2BL.1RS translocation line has high resistance to stripe rust, current prevalent fungus strain, powdery mildew and other important virus strains; the invention has excellent high yield character; the method lays an excellent germplasm base for the cultivation of breakthrough new wheat variety with disease resistance and high yield.

The invention relates to a selective breeding method for disease-resistant superior strains of paralichthys olivaceus, which comprises group selective breeding and family selective breeding of the paralichthys olivaceus and is characterized by also comprising molecular marker-assisted selective breeding, manual gynogenesis selective breeding or inbreeding selective breeding of a disease-resistant paralichthys olivaceus family. Multiple kinds of biotechnology such as group selective breeding, intraspecific hybridization, family selective breeding, molecular marker-assisted selective breeding, manual gynogenesis selective breeding, inbreeding and the like is comprehensively adopted; a selective breeding method for the disease-resistant superior strains of marine fishes in China is established for the first time by taking the paralichthys olivaceus as a material; the superior strains of the paralichthys olivaceus of which the vibrio anguillarum resistance is obviously improved are bred; and the breeding survival rate is improved by 20 to 47 percent and the growth rate is improved by over 20 percent. The method opens up a new technical approach for the selective breeding of the disease-resistant strains of the fishes, is suitable for popularization and application to all cultured fishes, has great significance and application value for breeding the disease-resistant superior strains of the cultured fishes, and can generate great economic and social benefits.

The invention discloses a selective breeding method of a high-resistance tobacco variety and belongs to the technical field of resistive breeding of tobacco. According to the selective breeding method, the tobacco high-yield varieties Yunyan 85, Yunyan 87 and Zhongyan 100 or K326 are taken as recurrent parents, and the cold-resistant variety Honghua Dajinyuan, the drought-resistant variety Zhongyan 14, the brown spot and blackleg resistant variety Zhongyan 86, the root-knot nematode-resistant variety TI1706, the common mosaic virus-resistant variety Taiyan No.7 and the tobacco bacterial wilt-resistant variety D101 as resistant resource parents, and the seven major drought, cold and disease resistant resource genes of the tobacco are pyramided and inserted into the good-quality high-yield tobacco variety with the same genetic background by use of a synchronous backcross and convergent cross method, so that a new high-resistance, high-yield and good-quality tobacco variety, which is highly resistant to 7 drought, cold and disease damage factors and highly consistent with the original recurrent parents in other agricultural and yield traits, is obtained.

The invention discloses a method for efficient selective breeding of a new odor type rice blast resisting rice variety. The method is characterized in that eurytopic and high-quality odor type rice restorer fengxianghui No. 1 is taken as a receptor parent, a rice blast resisting rice material containing rice blast resisting genes is taken as a donor parent, the conventional breeding technique is adopted, the high-throughput molecular marker-assisted selection technique, the rice anther in vitro culture technique and the human nose fragrance smelling and resistance field identification means are comprehensively utilized, a resistant improved odor type rice fengxianghui No. 1 restorer with stable fragrance, high rice blast resistance and excellent agronomic trait is prepared, and the odor type rice blast resisting rice variety is obtained through efficient selective breeding by means of combination and measuring matching of the resistant improved odor type rice fengxianghui No. 1 restorer and a sterile line. The method is efficient, quick and low in cost. Meanwhile, the traditional breeding technique and the modern molecular biological technique are effectively combined, the popularization and application values of the variety obtained through selective breeding are high, and the method can meet the requirement of enterprises for commercial breeding.

The invention relates to a microsatellite multi-PCR (Polymerase Chain Reaction) for a turbot paternity test, which comprises the steps of: extracting DNA from an individual turbot, screening pleomorphic microsatellite markers of the turbot, synthesizing a primer, constructing turbot multi-PCR systems and carrying out genealogy and paternity tests. In the method, two multi-PCR systems are established for the turbot genealogy and paternity tests, the steps of multi-PCR amplification, electrophoresis and silver staining are carried out on an individual to be tested so as to acquire microsatellite marker gene type data, cluster analysis for a genealogy test is carried out by adopting NTsys sofeware, and the paternity test is carried out by adopting Cervus2.0 software. The invention realizes that four microsatellite sites are simultaneously detected in one PCR, thus compared with the traditional PCR method, the efficiency of the method is improved by around 4 times, and the method can be subjected to the field test in a breeding base. The invention has the characteristics of high efficiency, economy, simplicity, convenience, easy operation and the like and can be popularized and applied in the aspects of germplasm identification, genealogy management and selective breeding of fine breeds for the turbots.

The invention discloses a high strength selecting and cultivating method of excellent stichopus japonicus seeds. The high strength selecting and cultivating method comprises the steps of carrying out cultivation, spawning or spermiation promotion, and hybridization on parent stichopus japonicuses from at least four different geographical groups, and carrying out selective breeding at different degrees in different periods according to a quantitative genetics method. By combining two traditional and effective methods of hybridization breeding and selective breeding, the invention preserves excellent properties of the stichopus japonicus and also avoids inbreeding; because the cultivated stichopus japonicuses have excellent properties of different groups, the cultivated stichopus japonicuses have the advantages of fast growth speed, strong stress resistance and the like; and the invention not only realizes seedling pre-culturing of the stichopus japonicus to meet market demand in advance, but also prolongs an effective growth period of the stichopus japonicus within a year, shortens cultivation time, and improves economic benefit; therefore, the inveniton is suitable for large-scaled factory production.

The present invention discloses one Penicillium citrinum strain with high nuclease P1 yield and its selective breeding process. The Penicillium citrinum strain is preserved in the preservation number of CGMCC No. 2014. Its selective breeding process includes low energy ion beam implantation on Penicillium citrinum spore suspension, transferring the mutagenized spore to plate culture medium for culturing, selecting single strain and transferring to malt wort and slant cultivating, liquid fermenting culturing to screen out strain with high nuclease P1 yield, and passage culturing for over 30 generations to obtain strain with stable high yield character. The Penicillium citrinum strain has high enzyme activity and stable high yield character, and is suitable for industrial production.

The invention discloses a molecular marker identification method of a paddy high nitrogen use efficiency gene, and belongs to the technical field of bioengineering. NRT1.1B is a high nitrogen use efficiency gene. According to the molecular marker identification method, a molecular marker composed of four primers is designed based on mononucleotide difference of wild type NRT1.1B gene with mutant type nrt1.1b gene, and is added into a same tube PCR reaction system so as to realize PCR amplification of DNA of different paddy plants; if characteristic strips 368bp and 243bp are obtained via amplification at the same time, the DNA is a homozygote containing NRT1.1B gene; if characteristic strips 368bp and 177bp are obtained via amplification at the same time, the DNA is a homozygote containing nrt1.1b gene, and if characteristic strips 368bp, 243bp, and 177bp are obtained via amplification at the same time, the DNA is a heterozygote containing NRT1.1B gene and nrt1.1b gene. The molecular marker identification method is rapid, convenient, and accurate in gene type detection of NRT1.1B gene; operation is simple; cost is low; the molecular marker identification method is extremely suitable for target gene identification of large-scale paddy materials; and important reference is provided for paddy resource gene identification and molecular marker-associated selective breeding.

The invention relates to a generative propagation breeding technology of dendrocalamus giganteus munro, comprising the following steps: seed collection and selection, seed storage, seed sowing and culture of seedling, preparation before planting bag seedling, planting, cultivation and protection, selective breeding of bamboo seedling, pest control, seed selection and survey of growth of the dendrocalamus giganteus munro. The sowing and the breeding of the invention has the characteristics of high reproduction coefficient, strong adaptability, strong stress resistance, ordered forest form, long life cycle, low afforestation expense, small labor intensity and the like.

The invention relates to a molecular marker for assisted selective breeding of upland cotton with greensickness resistant traits, belonging to the technical field of genetic breeding of cotton. Three SSR (Simple Sequence Repeat) markers for assisted selective breeding of the upland cotton resisting greensickness can be obtained by constructing a molecular marker genetic linkage map in upland cotton seeds to carry out QTL (Quantitative Trait Locus) positioning on the greensickness resistant traits and selecting remarkable SSR markers entering a linkage group section and the SSR makers linked with disease-resistant QTLs to screen different upland cotton plant systems. The three markers comprise marker primers and a primer combination and are suitable for greensickness-resistant molecular marker-assisted selective breeding of the upland cotton. The plant systems resisting the greensickness can be obtained by using the obtained molecular marker to carry out greensickness-resistant molecular marker-assisted selection on different upland cotton plant systems.

The invention discloses a selective breeding method of Yunnan manyleaf paris rhizome high-altitude cultivated species, which comprises the steps of: adopting different types of Yunnan manyleaf paris rhizome for domesticated planting, selecting germ plasm mateials which are good in adaptability to altitude and phenological period, relatively good in rootstock growth amount, relatively high in vegetative propagation capability and relatively good in plant characteristics as provenance for breeding, and further sieving and culturing cultivated species with relatively steady genes. The selective breeding method of Yunnan manyleaf paris rhizome high-altitude cultivated species not only improves purity and adaptability of high-altitude cultivated species, but also improves yield and adds economic income; compared with other provenances, the Yunnan manyleaf paris rhizome high-altitude cultivated species have relatively good generalization and planting foreground.

The invention discloses a selective breeding method of dark-shank partridge chickens. The method comprises the following steps: (1) selecting hens and cocks of 105 days or older from Guangxi Nandan-yao chickens to establish a family, cultivating a first male parental N line, and selecting cocks having characters meeting selection requirements as a first male parent; (2) selecting cocks and hens from Liuzhou partridge chickens to establish a family, cultivating a first female parental L line, and selecting hens having characters meeting selection requirements as a first female parent; (3) selecting cocks from Jiangxi Chongren partridge chickens and hens from Shaobo chickens, performing hybridization to obtain male parental hens, then performing hybridization on the mal parental hens and thecocks from the Nandan-yao chickens to obtain a dark-shank partridge chicken line group, establishing a family cultivation terminal male parent Q line, and selecting cocks having characters meeting selection requirements as a parent generation male parent; (4) performing mating on the first female parent L line hens and the first male parent N line cocks to obtain a parent generation NL line group, and selecting parent generation female parent hens according to requirements; and (5) performing mating on the parent generation cocks and hens to obtain commercial generation chicks namely a new line of dark-shank partridge chickens.