88 results about "Selective breeding" patented technology

The invention discloses an SNP (Single Nucleotide Polymorphism) site related to the number of spikelet per ear and the number of grains per ear of wheat and application of the SNP site. The SNP site comprises a kit for identifying or assisting in identifying the number of spikelet per ear and the number of grains per ear of wheat, primers and application of the elements in identifying or assisting in identifying the number of spikelet per ear and the number of grains per ear of wheat. The invention provides a new method for molecular marker-assisted selective breeding of wheat, and has important significance in cultivation and research of high-yield wheat varieties.

The invention discloses application of a KASP molecular marker in identification of light yellow flower color traits of muskmelons, and belongs to the technical field of molecular markers. According to the KASP molecular marker for identifying the light yellow flower color character of the muskmelon, a genetic segregation population is constructed by taking a dark yellow flower color muskmelon and a light yellow flower color muskmelon as parents, and a stably inherited marker site chr04: 26634798G / A is obtained through molecular marker technology identification. The marker is closely related to the light yellow flower color character of the muskmelon, and has the characteristics of strong specificity and good stability. The molecular marker provided by the invention can realize rapid genotype detection of a muskmelon material through a high-throughput genotyping system, and the identification result is accurate and reliable and is not influenced by environmental factors. The molecular marker can be applied to molecular marker-assisted selective breeding of melon flower color characters, the breeding efficiency is remarkably improved, the breeding period is shortened, the breeding cost is reduced, and an effective molecular breeding tool is provided for directional improvement of melon flower colors.

The invention discloses application of a KASP molecular marker in identification of sweet melon peel color traits, and belongs to the technical field of molecular markers. The invention aims to promote gene localization and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on sweet melon pericarp color characters. The KASP molecular marker is a KASP marker RW2; the KASP marker RW2 is designed according to an SNP (Single Nucleotide Polymorphism) site at the position of Chr04: 526653. According to the application, the KASP molecular marker RW2 is used for performing genetic typing on the red-peel muskmelon and the white-peel muskmelon, the DNA of a target plant can be identified only through one-time PCR amplification, the whole detection process is simple and convenient, and complex steps such as enzyme digestion, electrophoresis and sequencing are not needed. Through the high-throughput genotyping system, a genotyping graph and a genotyping value can be rapidly obtained, so that rapid identification of the genotype of a target plant is realized. The KASP molecular marker RW2 provided by the invention can be more efficiently applied to the breeding work of sweet melon peel color germplasm.

The invention discloses a chip for genome breeding and variety identification of tilapia mossambica. The invention provides a group of SNP (Single Nucleotide Polymorphism) marker combination for tilapia mossambica, which comprises 50000 SNP markers which are respectively SNP1-SNP50000 markers. According to the invention, the SNP marker combination related to economic characters of tilapia mossambica is integrated, representative sites with uniform coverage are selected, a biological probe for site typing is developed, and the site coverage, typing accuracy and GS accuracy of the SNP marker combination are basically consistent with those of re-sequencing; the method can be applied to the aspects of tilapia germplasm resource identification, genetic relationship identification, SNP typing, variety identification, molecular breeding, DNA fingerprint database construction, variety purity detection, germplasm resource genetic analysis, whole genome selective breeding, functional gene positioning, genetic map construction, genetic evolution analysis, whole genome association analysis and the like.

The invention relates to the technical field of genomics, molecular biology, bioinformatics and whole-genome selective breeding, in particular to a whole-genome liquid chip for apostichopus japonicus and application of the whole-genome liquid chip. The liquid phase chip contains sequences of background SNP loci and functional SNP loci which are used for gene analysis and are positioned on an apostichopus japonicus reference genome; wherein the functional SNP sites are associated with important economic characters of the apostichopus japonicus. The invention also discloses application of the liquid chip in apostichopus japonicus genome selective breeding, important economic character gene positioning, genetic diversity analysis, germplasm resource improvement and protection.

The invention discloses a molecular marker of a brassica napus salt-tolerant gene BnaA07.GRP3 and application of the molecular marker, and belongs to the technical field of rape breeding. According to the invention, two haplotypes hap0 and hap1 of the salt-tolerant gene BnaA07. GRP3 are found for the first time, a corresponding molecular marker is provided for the optimal haplotype hap1 of the BnaA07. GRP3, and the optimal haplotype hap1 of the BnaA07. GRP3 is specifically amplified, so that the salt-tolerant material of the brassica napus is identified. The salt-resistant material of the brassica napus can be quickly and accurately identified, and the repeatability is good. The method is applied to auxiliary selective breeding of salt-resistant characters of the brassica napus, the breeding efficiency of the salt-resistant brassica napus can be greatly improved, the selection range of salt-resistant materials is expanded, the method has huge application potential and prospects, and an ideal way is provided for cultivating the salt-resistant brassica napus.

The invention discloses a KASP molecular marker primer combination for identifying soybean hundred-grain weight character and application of the KASP molecular marker primer combination, and belongs to the technical field of soybean agronomic character molecular marker development and molecular marker assisted breeding. The invention discloses a KASP molecular marker primer combination for identifying the soybean hundred-grain weight character. The sequence of the KASP molecular marker primer combination is shown as SEQ ID NO.3-5. The invention provides a novel and simple molecular marker and an auxiliary selection method through the obtained KASP marker for regulating the hundred-grain weight character gene of the soybean, and is suitable for rapid screening of the relative height of the hundred-grain weight of the soybean and molecular marker auxiliary breeding. The molecular marker can detect genotypes of materials with different hundred-grain weights in natural populations, can also be used for molecular-assisted selective breeding of soybean materials with different hundred-grain weights, replaces large-scale phenotypic screening, reduces field workload, and greatly improves screening accuracy and efficiency.

The invention belongs to the technical field of molecular biology, bioinformatics, sorghum genetics and whole-genome gene chips, and particularly relates to a sorghum whole-genome SNP molecular marker combination developed based on a targeted sequencing technology, a 10K liquid chip and application of the sorghum whole-genome SNP molecular marker combination and the 10K liquid chip. The SNP molecular marker combination comprises 10,000 SNP loci, and the specific information of the 10,000 SNP loci is as shown in a table 1; the physical positions of the 10000 SNP loci are determined by performing sequence alignment based on a reference genome of the wine red tassel sorghum variety HYZ-T2T with the version number of v1.0. A liquid chip developed based on the SNP molecular marker combination provided by the invention can be effectively applied to genetic diversity analysis, whole genome association analysis, genetic typing, molecular marker-assisted selective breeding, whole genome selective breeding and other applications of sorghum germplasm resources.

The invention provides an InDel molecular marker sequence related to wheat drought resistance as well as a detection method and application thereof, and belongs to the technical field of molecular markers. The invention provides a polymorphic site for forming different drought resistance of wheat, the polymorphic site only has two haplotypes: a sequence of a 724bp long fragment as shown in SEQ ID No.2 is inserted between the fragments 245-246bp as shown in SEQ ID No.1, and the haplotype is homozygous as a haplotype A; the drought resistance of to-be-detected wheat which is not inserted with the 724bp long fragment sequence shown at the site, is haplotype B homozygous and is determined to be haplotype A homozygous is higher than that of a variety determined to be haplotype B homozygous, so that the drought resistance of the to-be-detected wheat is determined to be haplotype A homozygous by detecting the condition of the polymorphic site when the wheat is subjected to molecular marker-assisted selective breeding, and the drought resistance of the to-be-detected wheat is determined to be haplotype A homozygous. The wheat with relatively high drought resistance can be found.

The invention relates to the technical field of molecular biology, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker for sheep horn length regulation and control and application thereof. The SNP molecular marker is characterized in that T / C mutation exists at the site 35071069 of the No.17 chromosome of a sheep reference genome AR-UIRambv2.0, and T / C mutation exists at the site 35071069 of the No.17 chromosome of the sheep reference genome. The sheep horn length can be identified by using the SNP molecular marker provided by the invention, and breeding can be selectively carried out according to environmental adaptability.

The invention relates to the technical field of plant molecular genetic breeding, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker closely linked with white gourd pulp malic acid major QTL (Quantitative Trait Loci) and application thereof. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO.4, and G / A mutation exists at the 26th basic group of the sequence. The molecular marker and the white gourd pulp malic acid have a close linkage marker characteristic, so that a KSAP primer pair is developed on the basis of the SNP molecular marker. Results of the specific embodiment of the invention show that the KASP molecular marker provided by the invention can be used for identifying the content of malic acid in pulp of wax gourd seeds or early-stage seedlings with true leaves, and the method is high in accuracy, low in cost and short in consumed time. Therefore, the SNP molecular marker and the KASP primer pair provided by the invention can be used for molecular marker-assisted selective breeding of white gourds, and the process of white gourd flavor, taste and quality breeding is accelerated.

The invention relates to an SNP (Single Nucleotide Polymorphism) molecular marker associated with the fatness of turbots fed with fermented soybean meal and application of the SNP molecular marker, and belongs to the technical field of molecular markers. The SNP molecular marker obviously associated with the fatness character is obtained by analyzing the fatness character of the turbots through genome-wide association analysis and comprises at least one of six SNP loci; the scophthalmus maximus molecular marker can be applied to scophthalmus maximus molecular assisted breeding, screening is conducted on the genome level through the breeding method, the breeding period can be greatly shortened, dependence on phenotypic characters is reduced, the selective breeding efficiency is improved, and the scophthalmus maximus molecular marker has good application prospects.

The invention discloses application of an SNP (Single Nucleotide Polymorphism) marker influencing the lambing number of goats. The marker is located on a PIEZO1 gene on a capa circus 18 # chromosome, the specific SNP marker site is A / C base mutation at the 236bp position of SEQ ID NO: 1 in a sequence table, and the lambing number of CC genotype and AC genotype goat individuals at the site is remarkably higher than that of AA genotype individuals. According to the present invention, the Musheng black goats are adopted as the research object, the DNA sequence of the 33rd exon of the PIEZO1 gene is subjected to PCR amplification, the sequence has an SNP variation site, the influence of the site polymorphism on the goat lambing number is analyzed, the individual Musheng black goat breeding is performed according to the influence, and the method can be used for improving the Musheng black goat lambing number so as to provide the basis for the breeding of the Musheng black goats. The breeding of a new variety (strain) taking the Muscheng black goat as a breeding material is accelerated, and a marker resource is provided for marker-assisted selective breeding of goat lambing number traits.

The invention belongs to the technical field of molecular genetics, and particularly relates to a wheat powdery mildew resistance main effect QTL, a KASP molecular marker closely linked with the wheat powdery mildew resistance main effect QTL and application of the wheat powdery mildew resistance main effect QTL. The invention provides a wheat powdery mildew resistance major QTL, the major QTL locus is located at the end part of a wheat 1A chromosome short arm and is located in a 4.4 cM genetic interval between flanking KASP markers Chr1A6275522K2 and Chr1A11524652K9, nine KASP molecules are developed, auxiliary selective breeding and screening of powdery mildew resistance wheat strains can be carried out, and a new available molecular marker is provided for screening of wheat powdery mildew resistance materials.

This invention specifically relates to the detection and application of a CASP14 gene SNP marker related to sheep horn size, belonging to the field of genetic biology. The SNP marker affecting sheep horn traits described in this invention is located at locus 7944295 on chromosome 5, and its polymorphism is G / A. This SNP marker can be used to identify sheep horn traits, i.e., whether sheep have large or small horns, and selective breeding can be carried out based on environmental adaptability.

The invention relates to the field of molecular breeding of Chinese pears, and discloses an SNP (Single Nucleotide Polymorphism) marker related to the fruit peel color of Chinese pears, the SNP marker is located at the No.8 chromosome 4163889 site of a 20-century pear PPYr1.0 genome, the fruit peel color of the Chinese pears is green when the genotype is T / T, and the fruit peel color of the Chinese pears is brown when the nucleotide at the SNP site is C / C or C / T. Compared with the prior art, the SNP molecular marker has the following advantages and effects that genome re-sequencing and whole genome association analysis (GWAS) are utilized to enrich in a gene interval related to the fruit peel color of the Chinese pears, the SNP molecular marker closely linked with the target character is developed, the SNP molecular marker can be effectively used for molecular marker-assisted selective breeding of the Chinese pears, the accuracy of character selection of the fruit peel color of the Chinese pears is improved, and the SNP molecular marker can be applied to the molecular marker-assisted selective breeding of the Chinese pears. The breeding process is accelerated.

The invention discloses a TaSCL3-2A molecular marker and application of the TaSCL3-2A molecular marker in detection of drought resistance of wheat, and belongs to the field of TaSCL3-2A molecular markers. The technical problem to be solved by the invention is how to detect the drought resistance of wheat. The TaSCL3-2A molecular marker disclosed by the invention is nucleotides corresponding to the 814th to 878th sites of SEQ ID No.1 in a sequence table in a wheat genome, and the TaSCL3-2A molecular marker has insertion or deletion in the wheat genome. Experiments prove that the drought resistance of homozygous wheat in which nucleotides corresponding to the 814th-878th sites in SEQ ID No.1 in the sequence table are inserted is greater than that of homozygous wheat in which the sites are deleted. By adopting the molecular marker for detecting the drought resistance of the wheat, the drought-resistant wheat can be quickly and accurately found. The invention provides a new method for wheat drought-resistant molecular marker-assisted selective breeding, and has important significance in culture or research of stress-resistant wheat varieties.

The present invention discloses a molecular marker, primer and application thereof for identifying the color of Actinidia arguta fruit. The molecular marker of the present invention is located at 495bp upstream of the start codon of the AaLDOX gene promoter region of Actinidia arguta, which is a 29bp Indel variation. Utilizing the molecular marker and method of the present invention, the fruit color can be quickly and accurately identified in the childhood stage of Actinidia arguta by conventional molecular test detection, and the red and green Actinidia arguta germplasm resources can be efficiently distinguished. The method can be used for parent selection and seedling identification of offspring, greatly improving the efficiency and accuracy of selective breeding. The method of the present invention can be detected in each tissue, organ and each developmental stage of the plant, and has the advantages of being convenient and fast, having good specificity, high accuracy, and being unaffected by climate and environment. It can be applied to the precise, directional and efficient breeding of red Actinidia arguta, and has a large application value.

The invention discloses a plant quadrat investigation method based on deep learning, and the method comprises the steps: manually selecting a sampling scheme, carrying out the network connection of a sampling image transmission device and a carrier, and transmitting the sampling scheme; receiving picture data from the sampling picture transmission equipment and the carrier to obtain a sampling data set; analyzing the sampling data set to obtain a rough recognition result set; according to the sampling scheme, carrying out weight addition calculation on the rough recognition result set to obtain a high-precision recognition block, and analyzing the high-precision recognition block to obtain a final quadrat survey result; a species competition database is called according to the final quadrat investigation result, a simulation environment is constructed, and the final quadrat investigation result is introduced and a risk report is obtained; and generating an optional sampling scheme according to the final quadrat survey result and the risk report, and merging the optional sampling scheme into the sampling scheme.

The invention discloses an aquatic organism individual identification method based on a FishFaceID identification model, and belongs to the field of underwater target detection. The invention discloses an aquatic organism individual identification method based on Vision-Mama. The aquatic organism individual identification method comprises the following steps: S1, data acquisition and preprocessing; S2, FishFaceID identification model construction; S3, model training; according to the method, the FishFaceID always shows relatively high accuracy and robust cross-view performance in a plurality of evaluation scenes, and good balance is shown between the accuracy and the efficiency. The results prove the feasibility of deep learning in precision breeding application, such as individualized health monitoring, directional feeding and selective breeding; and meanwhile, the effectiveness of the proposed architecture under a real deployment condition is also highlighted.

The invention relates to the technical field of poultry genetic breeding and biology, in particular to a DGKA gene molecular marker for laying hen egg-laying persistent breeding, application and a method, and belongs to the technical field of molecular marker-assisted breeding. The molecular marker is located on a No.34 chromosome of a chicken reference genome bGalGal1. Mat.broil.GRCg7b, the 1334260th site has G / A polymorphism and is located in a 5 '-UTR region of a DGKA gene, and the SNP can be used as the molecular marker and is used for early genetic breeding of high-egg-yield persistent laying hens. The molecular marker is applied to early screening and genetic breeding of high-egg-yield persistent laying hens, breeding accuracy and breeding efficiency can be remarkably improved, phenotype determination cost is reduced, and the molecular marker has important economic value and popularization and application prospects for promoting efficient and precise development of the laying hen industry.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker combination for predicting the lysine content of fresh corn kernels and application of the SNP molecular marker combination, and belongs to the technical field of selective breeding of fresh corn. The SNP molecular marker combination provided by the invention is composed of 20000 SNP loci positioned on a corn B73 reference genome version v5. The SNP molecular marker combination provided by the invention can be used for molecular marker-assisted breeding of high-quality protein (high lysine content) fresh-eating corn, and can also be used for pyramiding breeding of multiple traits of fresh-eating sweet corn. According to the method for predicting the lysine content of the fresh corn kernels, the content of high-quality protein (lysine) of the fresh corn kernels can be predicted only by detecting the genotype of a breeding material, the method has the advantages of being clear in selection target and free of environmental influence, and meanwhile, the method can be used for predicting the lysine content of the fresh corn kernels. Prediction of the high-quality protein (lysine) content character of the grains is mainly carried out in the harvesting period of the fresh corn, and early detection can be achieved.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to sexual gland weight traits of fugu rubripes and application of the SNP molecular marker, and relates to the technical field of biology. According to the invention, association analysis is carried out in combination with the sexual gland heavy character of the fugu rubripes, and the SNP molecular marker significantly related to the sexual gland heavy character of the fugu rubripes is successfully excavated. The SNP molecular marker is used for breeding of improved gonad breeds of fugu rubripes, and compared with traditional phenotype-based selective breeding and cross breeding means, the SNP molecular marker has higher accuracy and detection efficiency and has a wide application prospect.

The present invention discloses a method for analyzing the selective breeding effects of corn by integrating population genetics and deep learning. The method collects corn germplasm resource data, including phenotypic data, genotypic data and environmental data; standardizes the phenotypic data and fills in missing values, and calculates population genetic parameters, including allele frequency, linkage disequilibrium and population structure stability; uses a deep learning model to perform association analysis between the population genetic parameters and the phenotypic data, uses the trained model to predict the phenotypic values ​​of candidate parents, calculates the breeding index in combination with the population genetic parameters, and provides guidance for corn breeding decisions based on the prediction results. The present invention achieves accurate prediction and evaluation of the selective breeding effects of corn by organically combining deep learning technology with the principles of population genetics, thereby improving breeding efficiency and providing a new technical path for corn variety improvement.

The invention discloses a gene molecular marker related to heat resistance of rhododendron pulchrum as well as detection and application of the gene molecular marker. Based on Rhododendron pulchrum field sample transcriptome sequencing and annotation, three SSR markers, namely a molecular marker 1, a molecular marker 2 and a molecular marker 3, which are remarkably related to heat resistance are screened out, corresponding specific primers are designed, and heat resistance genotypes are judged through PCR amplification and gel electrophoresis typing. The technology is further applied to breeding of the Rhododendron pulchrum heat-resistant strain. The invention also provides a kit related to breeding. The invention provides a new method for molecular marker-assisted selective breeding of Rhododendron pulchrum, and has important significance in cultivation and research of Rhododendron pulchrum adaptive to low altitude and high temperature.

The invention discloses application of an HAVCR2 gene in cattle molecular marker-assisted selective breeding, and belongs to the technical field of molecular genetics. The method comprises the following steps: by taking cattle genome DNA as a template, amplifying a cattle HAVCR2 gene copy number variation region by utilizing a specific PCR primer, amplifying a cattle BTF3 gene partial segment as an internal reference, and finally calculating and judging the individual copy number variation type by utilizing a 2 * 2-delta delta Ct method. The method provided by the invention lays a foundation for establishing association between cattle HAVCR2 gene copy number variation and growth and development, can be used for cattle molecular marker-assisted selective breeding work, and is convenient to popularize and apply.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to a pork quality trait gene THRSP, a primer pair and application of the SNP molecular marker and the primer pair, wherein the meat quality trait is intramuscular fat content; the molecular marker is located in a partial sequence of a pig THRSP gene, the nucleotide sequence of the molecular marker is shown as SEQ ID NO.1 in a sequence table, and a Ggt exists at the 141bp position of the sequence; and A basic group mutation. The invention also provides a primer pair for amplifying the molecular marker and a method for breeding by using the molecular marker. According to the invention, the correlation between one SNP molecular marker in the pig THRSP gene and the intramuscular fat content character of the pig is explored for the first time, so that the SNP molecular marker can be used for marker-assisted selective breeding of the pig, that is, the new application of the SNP molecular marker in the pig THRSP gene in marker-assisted breeding of the pork quality character is provided, the early screening of the pork quality character is realized, and the screening method is simple and rapid.

The present disclosure relates at least in part to genetic engineering of vegetable (e.g., tomato) plants to, for example, produce heme, a molecule contributing to a meat-like flavor, and to the incorporation of male sterility in these plants to facilitate the production of F1 hybrid seeds. Described herein, in some embodiments, are methods for constructing and introducing into such plants genetic constructs containing both a heme gene and a male sterility gene, the resulting plants, and the use of these plants in hybrid seed production. The present disclosure also provides at least in part methods for enhancing lycopene content in plants, such as tomato plants, either through genetic modification or selective breeding. While introducing key genes like Psy is effective, adding multiple genes or knocking out competing pathways can lead to more substantial increases in lycopene levels. Enhanced lycopene content can provide added health benefits, including potential cancer protection, making these plants, such as tomatoes, particularly valuable in the context of functional foods. The plants may be of varieties with high lycopene content combined with genetic modifications as provided herein. The present disclosure offers a comprehensive solution for producing plants (e.g., tomatoes) with increased nutritional value.

The invention discloses a molecular marker related to the first laying day age of Jianghan chicken, a primer pair and application of the molecular marker and the primer pair, and belongs to the technical field of molecular marker-assisted selective breeding. The molecular marker is located at a first exon sequence of a chicken PCDHA1 gene and has a sequence as shown in SEQ ID NO.3, fragment repetition exists in the sequence, a base composition at the 54bp position of the sequence SEQ ID NO.3 is C or T, a base composition at the 56bp position of the sequence SEQ ID NO.3 is C or G, the polymorphism of the laying day age of the Jianghan chicken is embodied, when the base composition at the 54bp position and the base composition at the 56bp position of the sequence SEQ ID NO.3 is CC type, the laying day age of the chicken is late, and the polymorphism of the laying day age of the Jianghan chicken is determined. If the basic group at the 54bp and the basic group at the 56bp form a TG type, the chicken is early in first laying day age. When the method is used for breeding the Jianghan chickens, the breeding efficiency can be remarkably improved, the breeding period can be effectively shortened, the breeding accuracy is high, and the cost is low.

The invention belongs to the technical field of fish breeding and biochips, and particularly relates to a micropterus salmoides SNP (Single Nucleotide Polymorphism) chip and application thereof. A specific capture probe is designed based on the provided largemouth bass SNP molecular marker combination, a gene chip for whole genome genotyping of largemouth bass is constructed, the gene chip is used for accurate and comprehensive detection of whole genome genotyping of largemouth bass, and the gene chip has the advantages of high detection flux, low cost, flexible customization and the like; the method is used for germplasm resource identification, genetic diversity analysis, whole genome association analysis, important functional gene mining and selective breeding of the micropterus salmoides, and a novel and efficient tool is provided for accelerating fine variety breeding of the micropterus salmoides.