151 results about "Penicillium citrinum" patented technology

The invention relates to a penicillium citrinum sourced citrinin compound (penicitrinol O) as well as a preparation method and an application thereof. The compound has an effect on inhibiting tumor cell proliferation and has anti-tumor activity, and the structural formula is shown in the specification. The preparation method comprises the steps: fermenting and culturing penicillium citrinum IBPT-5 to obtain a fermented product; and then separating and purifying the fermented product to obtain the compound. The experiment shows that the compound has high anti-tumor activity for human cervical cancer cell line hela and can be used for preparing cell proliferation inhibition medicines or anti-tumor medicines for the study on anti-tumor.

The invention discloses a nano silver antibacterial latex pillow or mattress and a preparation method thereof. Tiny air holes are fully distributed on the surface of the nano silver antibacterial latex pillow or mattress, and air is filled into the tiny air holes; and the silver antibacterial latex pillow or mattress has bacterial resistance on aspergillus niger, aspergillus flavus, discolored aspergillus, penicillium citrinum, paecilomyces varioti, herbarium cladosporium, trichoderma viride and chaetomium globasum. The preparation method comprises the following steps of: uniformly mixing nano sliver with natural latex; then respectively adding sulphur, bi(triethoxy propylsilane)tetrasulfide and 2,6-butylethylene-4-methylphenol; uniformly stirring; adding soap and air to generate foams; then adding gelatin; and compressing for moulding. The nano silver antibacterial latex pillow or mattress of the invention combines air permeability, high elasticity and soft touch feeling of the latex with efficient bacterial resistance and broad spectrum sterilizing performance of nano silver and can effectively overcome the defects of easy bacterial generation, difficult air permeation, and the like of a common pillow on the market.

The invention relates to a process for producing 5'nucleotide. A penicillium citrinum strain M-71 is cultured by submerged fermentation to generate extracellular nuclease P1, 2.5 to 5 percent ribonucleic acid solution is degraded, of which, the degradation rate is more than 80 percent; foreign protein and residual ribonucleic acid are removed by using a high frequency vibrating screen, clean 5' nucleotide solution after impurity removal is subjected to ultrafiltration by a ceramic membrane to remove the macromolecular residual matters; and four columns filled with strong alkaline anion exchange resin are connected in series to one-time adsorb and elute and collect in multiple steps, four mononucleotide solutions are collected, and a nano filter membrane with the molecular weight of 300 daltons is selected for cutting, desalting, concentration, decoloration by active carbon, crystallization and drying to obtain the product. The process has the advantages of better separating four nucleotides, removing inorganic phosphorus by elution and ensuring the preparation of high-purity 5'nucleotide product, and according to HPLC tests, the purity of the product can reach between 98 and 102 percent.

The invention relates to novel anti-tumor application of an alkaloid compound penicillium enol A1 from penicillium citrinum. The penicillium citrinum IBPT-5 is collected in the China Center for Type Culture Collection (CCTCC), which is located in Wuhan University and has the collection number of CCTCC NO:M2013713, on December 25, 2013. Experiments prove that the compound has better anti-tumor activity on various tumor cells, and can be used for preparing cell proliferation inhibition medicines or anti-tumor medicines for anti-tumor study, wherein tumor cells comprise human colon cancer cells SW620, human hepatoma cells Huh7, human gastric carcinoma cells BGC-823, human colon cancer cells SW480, human esophageal squamous carcinoma cells KYSE450, human esophageal cancer cells EC9706, human highly metastatic lung carcinoma cells 95-D, human hepatoma cells PLC and human gastric carcinoma cells HGC-27.

The present invention discloses one Penicillium citrinum strain with high nuclease P1 yield and its selective breeding process. The Penicillium citrinum strain is preserved in the preservation number of CGMCC No. 2014. Its selective breeding process includes low energy ion beam implantation on Penicillium citrinum spore suspension, transferring the mutagenized spore to plate culture medium for culturing, selecting single strain and transferring to malt wort and slant cultivating, liquid fermenting culturing to screen out strain with high nuclease P1 yield, and passage culturing for over 30 generations to obtain strain with stable high yield character. The Penicillium citrinum strain has high enzyme activity and stable high yield character, and is suitable for industrial production.

The invention relates to a preparation method and a use of two dimer citrinin compounds. The dimer citrinin compounds with novel structures are produced by adopting Penicillium Citrinum HGY1-5 in samples collected from a crater. Tests prove that the compounds can be used as a cell proliferation inhibitor, an apoptosis inducer or an anti-tumor agent.

The invention relates to a waterborne hinokitiol wood preservative and a preparation method thereof, and is used for solving the problems that hinokitiol giving a natural corrosion resistance to wood has poor water solubility and is difficult to use. The wood preservative comprises the components according to mol ratio: 0.2-2 mol of hinokitiol, 1 mol of a solubility promoter, and 0.01-1 mol of a tannin extract (the molar ratio calculated according to the content of phenolic hydroxyl group). The wood preservative is used for processing the wood, and when the mass concentration of hinokitiol is about 1%, the resistance of the processed wood to Aspergillus niger, Penicillium citrinum, Trichoderma is up to 100%, and the fungal growth is completely inhibited; and moreover, the molding resistance of the processed wood to Coriolus versicolor and Gloeophyllum trabeum can achieve strong molding resistance level.

The invention relates to a method for detecting fungaltoxin by using a photonic crystal microsphere flow type chip. According to the method, a photonic crystal microsphere is used as a fungaltoxin probe carrier; the surface of the microsphere is directly subjected to competitive immune, so that a plurality of fungaltoxins are detected; aflatoxin, Fumonisins and penicillium citrinum toxin are used as detection objects; a fluorescein isothiocyanate marked antibody competes with an antigen fixed on the surface of the microsphere to obtain own antigens; decoding is performed by a confocal fluorescence scanner and a reflection spectrum of a photonic crystal; and the three fungaltoxins can be detected at the same time. According to the method, the detection limit to the aflatoxin, the Fumonisins and the penicillium citrinum toxin is 0.001 ng/mL; the linear detection range is 0.001 to 10 ng/mL; only 10 mu L test sample is needed; and the whole analysis process is shorter than 3 hours.

The invention discloses an immobilization method of penicillium citrinum, which comprises the steps that a pretreated fiber material serves as an immobilization medium; a production strain, namely the penicillium citrinum of nuclease P1 is activated, inoculated into a fermentation container, and cultured for 24-60h, so that the strain is adsorbed on the fiber material in a culturing process. In the method, the fiber material used by an immobilization cell is pretreated correspondingly, and is low in cost, nontoxic, easy to obtain and simple in preparation process when serving as an immobilization material; the method is suitable for large-scale production. The pretreated fiber material has larger porosity and specific surface area, so that cells immobilized in unit volume are more, the advantage of high density fermentation is ensured, the fiber material has good mass transfer property, and a fermentation period is shortened.

The invention relates to a citrinin compound penicitrinol L derived from Penicillium citrinum, a preparation method and application of the citrinin compound penicitrinol L. The citrinin compound has the effects of inhibiting tumor cell proliferation and has antineoplastic activity. The structural formula of the citrinin compound is shown in the formula (refer to the Specification). Through performing fermental cultivation on Penicillium citrinum ((i)Penicillium citrinum (/i)) IBPT-5 to obtain a yeast and conducting separation and purification on the yeast, the compound is obtained. The experiment confirms that the compound has a better antineoplastic activity to human cervical carcinoma cell line hela, so that the citrinin compound penicitrinol L can be used for preparing cell proliferation suppressing drugs or antineoplastic drugs and used in antineoplastic researches.

The invention discloses a method for preparing nuclease P1 through penicillium citrinum semi-continuous fermentation. A fermentation culture medium containing vitamin B substances is used for preparing the nuclease P1 through the penicillium citrinum semi-continuous fermentation. According to the method disclosed by the invention, the single batch fermentation time is shortened from 60 hours to 20 hours, the enzyme activity is improved from 3000 U/mL to 6500 U/mL, and more than 12 batches can be continuously fermented. With the adoption of the method, the fermentation time can be obviously shortened, and the production cost can be reduced.

The invention relates to an extraction method of a mycelial fungus genome. Enzymolysis is performed on Penicillium chrysogenum, Aspergillus niger, Trichoderma reesei, penicillium citrinum and other mycelial fungus hyphae by using a helicase-cellulase mixed enzyme solution, and the enzymolysis treatment is effectively combined with ultrasonic treatment and high-temperature heating treatment, thereby obtaining the mycelial-fungus-genome-containing supernate for PCR (polymerase chain reaction) identification. The supernate of the mycelial fungus hypha enzymolysis solution obtained by the extraction method is directly used for PCR reaction to obtain the mycelial fungus 18S rDNA gene strip without purification operation. The operational process is simple, safe and stable, avoids the use of the toxic extraction reagent, and reduces the injury to the experimental personnel.

The invention provides a fermentation method for preparing nuclease P1, which using directly penicillium citrinum sporogon as seed, fermenting by means of fed-batch sugar supplement to improve activity of the nuclease P1, the mehod without first and second order seed culture shorts production cycle greatly and raises efficiency. The invetion also uses obtained enzyme for preparing 5'-mononucleotide with 85-90% rate of enzymatichydrolysis and 15 mg/ml of producing rate of nucleotide. The invention also provides a penicillium citrinum 3.2788-Y01 of nuclease P1, which fungus stroing number is CGMCC NO.1943, the stem can raise one times active unit of the nuclease P1.

The invention discloses a Penicillum oxalicum, and applications thereof. The preservation name of the Penicillum oxalicum is Penicillum oxalicum SG4, and is preserved on 30th, April, 2015, in China Center For Type Culture Collection, in Wuhan university, Wuhan, China; and preservation number is CCTCCM 2015270. It is confirmed by experiments that the Penicillum oxalicum SG4 possesses strong inhibition effects on orange Penicillium citrinum, and can be used for preparing orange biological fresh-keeping agents; a novel direction is provided for development of novel biological fresh-keeping agents and orange biological preservation; and excellent economic and social benefits are achieved.

The invention discloses a food natural compound preservative prepared from chitosan oligosaccharides, and relates to the technical field of food storage. The preservative comprises the following raw materials in percentage by mass: 18 to 26 percent of tea polyphenols, 6 to 10 percent of epsilon-polylysine, 21 to 38 percent of glycine, 2 to 4 percent of allicin, 15 to 25 percent of chitosan oligosaccharides, and 15 to 21 percent of cassia bark extract. According to the invention, the preservative effect of the obtained natural compound preservative is evaluated, and escherichia coli, bacillus subtilis, staphylococcus aureus, salmonella typhimurium, saccharomyces cerevisiae, aspergillus niger and penicillium citrinum are selected to evaluate the preservative effect, and the preservative hashigh eating safety. The natural compound preservative of the invention has strong bacteriostasis effect on common bacteria and fungi in foods, can be applied to foods without influencing original flavor and color, and has wide application range. The natural compound preservative of the invention is safe, nontoxic, efficient and practical.

The invention discloses a sophora tonkinensis endophytic fungus SDTE-P. The sophora tonkinensis endophytic fungus SDTE-P is classified and named as Penicillium citrinum SDTE-P, wherein the ITS sequence of the strain is as described by SEQ ID NO.1, the depository authority is the General Microbiological Collection Center of China Committee for Culture Collection of Microorganisms, the depository address is Institute of Microbiology, Building 3, No.1, Beichen West Road, Chaoyang District, Beijing, the depository date is 13 May 2015, and the depository number is CGMCC No.10808. According to the sophora tonkinensis endophytic fungus SDTE-P provided by the invention, a strain of endophytic fungus Penicillium citrinum SDTE-P is separated and screened from the root of a medicinal plant sophora tonkinensis for the first time, the Penicillium citrinum SDTE-P has a strong inhibiting effect on panax notoginseng fusarium solani, and a wide application prospect is created for the biological prevention and control field of panax notoginseng fungal diseases.

The invention discloses a gene highly expressing a nuclease P1 and an expression vector and a host cell comprising the gene and an application for the gene. The invention discloses a recombinant pichia yeast CGMCC No.3188 expressing the gene of nuclease P1 produced from Penicillium citrinum and its construction method. The inventive recombinant engineering strain pichia yeast CGMCC No.3188 can highly express Penicillium citrinum nucP gene. The secretion-expressed nuclease P1 has high phosphodiesterase activity, is used for hydrolyzing RNA, has high product yield and little generated impurity and reduces working procedure of downstream separation as well as decreases production cost. The invention uses the recombinant engineering strain pichia yeast CGMCC No.3188 to perform high density fermentation. The enzyme activity is 1.5 times by the wild strains. The acquired recombinase is applied to RNA solution enzymatic hydrolysis, thus, reaching the hydrolysis rate of more than 40% and the acquired hydrolysate by-product is substantially reduced.

The invention relates to an antitumor application of penicillium enol A2 from penicillium citrinum. Penicillium citrinum IBPT-5 is preserved at the China Center for Type Culture Collection on December 25, 2013; the address is Wuhan University; and the preservation number is CCTCC NO:M 2013713. An experiment proves that the compound has relatively good antitumor activity on a plurality of tumor cells, and can be used for preparing cell proliferation inhibition drugs or antitumor drugs to carry out antitumor research; and the tumor cells comprise human colon cancer cells SW620, human hepatoma carcinoma cells Huh7, human colon cancer cells SW480, human esophageal squamous cancer cells KYSE450, human esophageal carcinoma cells EC9706 and human hepatoma cells PLC.

The invention provides marine penicillium citrinum antifungal protein PcPAF and a preparation method thereof, and relates to microbial original antifungal protein. Marine penicillium citrinum W1 is stored as CCTCC (China Center for Type Culture Collection) NO: M2013205. According to the preparation method, supernatant of fermentation broth of marine penicillium citrinum W1 is separated and purified to obtain antifungal protein, naming PcPAF; the identification result shows that the obtained mass spectrometry information is failed to be matched in NCBI (National Center of Biotechnology Information) Genbank database; 15 amino acids at the N end of the protein are detected by an N end sequencing manner; by being compared with the NCBI Genbank database, the obtained amino acid sequence shows low homology, so that PcPAF can be determined to be a novel antifungal protein. By the N end sequencing, the 15 amino acids at the N end are AGNRPDFPRRHHPGG. PcPAF shows obvious effect on inhibiting pathomycete, namely, trichoderma viride and the like, of four plants, and remains the antimicrobial activity after being heated for 20 minutes at 80 DEG C.

The invention relates to a preparation method and application of penicillium citrinum-derived penicitrinine A. The compound has the effect of restraining human nasopharyngeal carcinoma cell proliferation. The structural formula of the compound is shown in the description. According to the invention, through fermenting culture of penicillium citrinum IBPT-5, a fermentation product is obtained, and then the compound is separated and purified from the fermentation product. Experiment results confirm that the compound has better antitumor activity on human nasopharyngeal carcinoma cells such as CNE-1 and CNE-2. The penicillium citrinum-derived penicitrinine A can be applied to preparation of human nasopharyngeal carcinoma cell proliferation inhibition drugs or antitumor drugs to be used for research on human nasopharyngeal carcinoma.

The invention relates to the technical field of microbial fertilizer, and specifically relates to phosphate-solubilizing penicillium PSF. The strain is named, in classification, as PSF and belongs toPenicillium sp., namely to Penicillium citrinum; and the phosphate-solubilizing penicillium PSF is deposited with the China General Microbiological Culture Collection Center on Oct. 9th, 2019 and assigned accession number CGMCC No. 18558, and the deposited address is No. 3, No. 1 yard, West Beichen Road, Chaoyang District, Beijing 100101. The provided phosphate-solubilizing penicillium PSF has good dissolution effects on various insoluble phosphate such as Ca5(PO4)3OH, AlPO4, Zn3(PO4) and FePO4; and the phosphate-solubilizing penicillium PSF has high salt tolerance and can well grow and colonize in salinized soil so as to make insoluble phosphorus converted into dissolved phosphate that can be absorbed and utilized by plants, so that the growth of the plants can be promoted, the using amount of chemical fertilizer can be reduced, and therefore, the phosphate-solubilizing penicillium PSF can have good application and development prospects.

The invention discloses a preparation for preventing and treating post-harvest mycosis of fruits, and belongs to the field of post-harvest fresh-keeping. The preparation consists of 0.08-0.15% boric acid and 0.8-1.2% chitosan oligosaccharide. According to the invention, the boric acid and chitosan oligosaccharide composite preparation has a better bacteriostatic effect on penicillium expansum, byssochlamys and penicillium citrinum. Compared with single treatment of the chitosan oligosaccharide or the boric acid, the chitosan oligosaccharide and the boric acid can generate a synergistic effect,can effectively inhibit the growth of spores and hyphae, and have a better bactericidal effect. In addition, the preparation of the invention obviously improves the hardness of fruits, maintains highcontent of soluble solid matters in the fruits, obviously reduces the incidence rate of the fruits, reduces the occurrence of mildew, improves the fresh-keeping effect, and prolongs the preservationtime.

The invention relates to a strain of Penicillium citrinum LJ318 capable of degrading chlortetracycline and belongs to the technical field of microorganisms. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC) in March 3rd, 2012, and the preservation number of the strain is CGMCC No.5850. An rDNA-ITS (Ribosomal DNA internal transcribed spacer) gene sequence of the chlortetracycline degrading strain LJ318 consists of 544 basic groups. The strain is wide in chlortetracycline-tolerating range and has relatively good degradation effects on the residual chlortetracycline in both liquid and solid wastes.

The invention discloses a sophora tonkinensis endophytic fungus SDTE-P. The sophora tonkinensis endophytic fungus SDTE-P is classified and named as Penicillium citrinum SDTE-P, wherein the ITS sequence of the strain is as described by SEQ ID NO.1, the depository authority is the General Microbiological Collection Center of China Committee for Culture Collection of Microorganisms, the depository address is Institute of Microbiology, Building 3, No.1, Beichen West Road, Chaoyang District, Beijing, the depository date is 13 May 2015, and the depository number is CGMCC No.10808. According to the sophora tonkinensis endophytic fungus SDTE-P provided by the invention, a strain of endophytic fungus Penicillium citrinum SDTE-P is separated and screened from the root of a medicinal plant sophora tonkinensis for the first time, the Penicillium citrinum SDTE-P has a strong inhibiting effect on panax notoginseng colletotrichum gloeosporioides, and a wide application prospect is created for the biological prevention and control field of panax notoginseng fungal diseases.