84 results about "Chlortetracycline" patented technology

The invention discloses a method for synthesizing tigecycline. The method comprises: 6-demeclocycline is subjected to catalytic hydrogenation, nitration, selective deoxidization, amino prevention reaction, catalytic deoxidization, and a deprotection to form 9-amino minocycline hydrochloride; the 9-amino minocycline hydrochloride reacts with tert-butylamine acetyl chloride hydrochloride to form tigecycline. The invention uses 6-demeclocycline as a starting material, without synthesizing minocycline as the intermediate. The novel method has simple operation, easily controllable conditions, low requirements on equipment, low pollution, and great application prospect.

The invention relates to Trichoderma LJ245 capable of degrading aureomycin, belonging to the technical field of the microorganism. The 16S rDNA full sequence determination shows that: the strain LJ245 is Hypocrea lixii with the preservation number CGMCC No.5753. The strain LJ245 grows fast; mycelium is in the white flocculence and at the late growth stage, the mycelium is green yellow due to generation of conidium. The microscopic observation shows that: the mycelium is septate, colorless conidiophore vertically grows out and the conidium is spherical. The strain LJ245 has the function of degrading the aureomycin remained in the strain dregs and waste water, is also applied in treating the wastes from the aureomycin production enterprise, is also expected to be applied on the biological repair of the aureomycin polluted environment, especially is used as a sole carbon source, moreover the strain still grows well when the concentration of the aureomycin is low to 0.01g/L, thus the residual aureomycin is reduced to the hilt. Simultaneously, the strain is conveniently used and also used as the preparation raw material of an aureomycin degradation strain agent.

The invention provides a fermentation technology for increasing the aureomycin yield by adding metal ions. Certain amounts of zinc sulfate and ferric trichloride are added into a fermentation culture medium of aureomycin, thus achieving an objective of increasing the aureomycin yield. According to the technology, 500 ppm of the zinc sulfate and 1 ppm of the ferric trichloride are added into the conventional fermentation culture medium. The fermentation level by the fermentation technology provided by the invention is higher than the original fermentation level. The pilot potency and the yield are both increased, thus laying good foundations for industrial scale-up and application in the next stage.

The invention discloses a method for high throughput screening of chlortetracycline high-yield strains and belongs to the technical field of metabolic engineering. Through a comparison of growth and yield of streptomyces aureofaciens on five flat plate culture mediums, an optimal culture medium is selected, and guarantee is provided for starting strains. Through a comparison of two primary screening methods which are color reaction between chlortetracycline and Sm3+ and a colorimetric method of co-heat of chlortetracycline and hydrochloric acid, the simple and accurate color reaction is selected as the primary screening method. Repeatability and accuracy of nitric acid color reaction serving as a primary screening means are verified.

The invention relates to the field of fermentation production processes, and discloses a chlortetracycline fermentation production method using refined protein powder. According to the present invention, Streptomyces aureus is adopted as a starting strain, and pure culture and two-stage fermentation are performed to produce chlortetracycline; with the application of the 100% refined protein powder to replace the soybean cake powder, the chemical titer is increased by 2.3% compared to the application of the soybean cake powder, and the yield is increased by 1.8% compared to the unimproved method, such that the yield is effectively increased; and the raw material cost input per tank can be reduced by about 540 yuan after the cost accounting, the effect is good, the fermentation production level is stably increased, and the theoretical and applied basis is provided for the industrial production of the antibiotics and the scientific research.

The invention discloses oil-in-water compound chlortetracycline nanoemulsion, and belongs to the technical field of medicines. The oil-in-water compound chlortetracycline nanoemulsion comprises the following components in parts by weight: 1-18 parts of aqueous magnolia officinalis extract, 25-45 parts of a surfactant, 0-20 parts of a co-surfactant, 1-20 parts of chlortetracycline, 1-20 parts of oil and 20-70 parts of water. The oil-in-water compound chlortetracycline nanoemulsion has relatively narrow emulsion particle size distribution, is transparent, has high stability, relatively low surface tension and good mobility, and is convenient to take; a nanoemulsion preparation improves solubility and permeability of the chlortetracycline, so that the bioavailability of a medicine is improved; after the aqueous magnolia officinalis extract and the chlortetracycline are compounded, antibacterial abilities of the aqueous magnolia officinalis extract and the chlortetracycline are mutually promoted and enhanced, so that the antibacterial effect of the oil-in-water compound chlortetracycline nanoemulsion is more stable.

The invention discloses an aureomycin strain breeding culture medium and a strain breeding method. The culture medium comprises, by weight, 6.0%-7.5% of corn starch, 3.5%-4.5% of soybean cake powder, 0.15%-0.25% of yeast powder, 0.55%-0.65% of calcium carbonate, 0.3%-0.4% of ammonium sulfate, 0.2%-0.3% of sodium chloride, 0.02%-0.03% of magnesium sulfate, 0.02%-0.03% of monopotassium phosphate, 2.86%-4.28% of soya-bean oil and the balance water. An aureomycin strain breeding culture medium formula is improved, a strain culturing and strain fermentation cultivation second-level fermentation mode is changed into a primary fermentation mode, strain breeding time is greatly shortened, strain breeding efficiency is improved, the using quantity of shaking flasks and shaking tables is reduced, cost is lowered, the probability of strain pollution is lowered, and accordingly the culture medium and the method are suitable for large-scale strain screening.

The invention provides a technology method used in an aureomycin fermentation production process by utilizing mycoprotein in place of a portion of yeast powder. The mycoprotein is added into an aureomycin basal culture medium according to a certain ratio to replace the portion of the yeast powder, thus achieving an objective of reducing the production cost of aureomycin. The method is characterized in that culture medium aqueous solution per liter in a fermentation tank in a fermentation preparation process comprises following components: 20 g of groundnut meal, 10 g of soybean cake powder, 60 g of the corn starch, 0.1 g of amylase, 2 g of sodium chloride, 7.3 g of ammonium sulfate, 5.7 g of calcium carbonate, 4.35 g of the yeast powder, 4.35 g of the mycoprotein, 13.3 g of corn steep liquor, 0.22 g of magnesium sulfate, 0.1 g of potassium dihydrogen phosphate and 0.17 g of soybean oil. When the basal culture medium sterilization is completed and the temperature is decreased to 30-35 DEG C, a second-stage seed is transferred into the fermentation tank. After seed transference, fermentation production is controlled by changing temperature. The culture pressure is controlled at 0.01-0.04 MPa. The fermentation culture viscosity is controlled at 1-35 s. The fermentation period is 90-124 h. According to the method, the mycoprotein is adopted in the fermentation process to replace 50% of the yeast powder, the production is almost the same as that of common fermentation, products are qualified and the cost of raw materials for one tank is reduced by 1040 Yuan, and therefore the cost of the raw materials is reduced in the case of guaranteeing production stability.

The invention relates to an Aspergillus oryzae LJ366 strain used for degrading aureomycin and belongs to the microbial technical field. The bacterial strain has already been preserved in the China General Microbiological Culture Collection Center on Aug 27, 2013 and the preservation number is CGMCC No.8078. The ITS gene sequence of the Aspergillus oryzae LJ366 strain used for degrading the aureomycin is composed of 593 bases. The bacterial strain can degrade the aureomycin and can be used for processing residual aureomycin in solid waste and liquid.

The invention aims at providing rumen bypass chlortetracycline micro-pills and a preparation method thereof. The rumen bypass chlortetracycline micro-pills sequentially comprise drug-containing pill cores and coating layers from inside to outside, wherein the drug-containing pill cores are prepared from, by weight, 10-25 parts of chlortetracycline, 10-30 parts of corn starch, 30-40 parts of calcium carbonate, 0.8-3.2 parts of hydroxypropyl methylcellulose, 5-10 parts of adhesive and 7-25 parts of coating layer. The coating layers of the rumen bypass chlortetracycline micro-pills prepared by means of the preparation method are complete in rumen, and no chlortetracycline is released; then the micro-pills reach the stomach, the coating layers are damaged in a gastric acid environment, the chlortetracycline is released, and accordingly antibacterial and growth-promoting effects are achieved; meanwhile, the physiological habits of ruminants are not affected, and the micro-pills are convenient to use.

The invention relates to a Penicillium spinulosum LJ220 strain for degrading aureomycin, belonging to the technical field of microorganism. The Penicillium spinulosum LJ220 strain disclosed herein is preserved in China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.5754 on 14 Feb, 2012. The gene sequence of the strain is obtained by genome DNA extraction, rDNA-ITS sequence-based amplification and complete sequence determination and analysis. The LJ220 strain can grow in a medium with aureomycin as the sole carbon source. The strain is convenient for usage, and has efficient degradation on residual aureomycin in bacteria residues.

The invention relates to a swine feed and specifically relates to a fattening swine feed. The swine feed comprises the raw materials in parts by weight: corn 650-700, soybean meal 50-60, stone powder 10-13, cottonseed meal 40-50, fish meal 1-5, chlortetracycline 0.3-0.6, betaine 0.7-0.9, choline 0.8-1.2, compound enzyme 0.8-1.2, salt 1-1.5, vitamin C 0.01-0.04, vitamin D 0.5-0.8, vitamin B6 0.5-0.8, vitamin B12 0.4-0.6, vitamin E 0.7-0.9, Radix Astragali 10-15, Argy Wormwood leaf 10-15, Chinese Arborvitae 7-10 and pine needle powder 4-7.

The invention relates to a strain of Penicillium citrinum LJ318 capable of degrading chlortetracycline and belongs to the technical field of microorganisms. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC) in March 3rd, 2012, and the preservation number of the strain is CGMCC No.5850. An rDNA-ITS (Ribosomal DNA internal transcribed spacer) gene sequence of the chlortetracycline degrading strain LJ318 consists of 544 basic groups. The strain is wide in chlortetracycline-tolerating range and has relatively good degradation effects on the residual chlortetracycline in both liquid and solid wastes.

The invention discloses a preparation method of chlortetracycline sustained-release microspheres. The method comprises steps as follows: mixing chlortetracycline with montmorillonite, adding the mixture to water to prepare a suspension, adding the suspension to a sodium alginate solution, evenly mixing the mixture, dropping the mixture into a calcium chloride solution for crosslinking, and collecting and air-drying gel spheres to obtain the chlortetracycline sustained-release microspheres. The condition of mixing of the chlortetracycline-montmorillonite suspension with the sodium alginate solution is optimized, the problems that mixtures are higher in viscosity and difficult to stir are well solved by the aid of high-speed magnetic stirring and even mixing, the mixture is evenly dispersed through full stirring, and stabilization of the chlortetracycline and formation of the sustained-release microspheres are facilitated. Besides, the drying method is also optimized, defects of other drying methods are overcome due to adoption of an ethanol soaking and air-drying method, the dried microspheres are regular in form, the drying time is shorter, and soaking of the microspheres with ethanol and the room temperature environment are beneficial to stabilization of properties of the chlortetracycline.

The invention relates to swine-origin lactobacillus reuteri and prepared solid probiotics. The bacterial strain is separated from fresh night soil of healthy live pig, and is indentified as Lactobacillus reuteri YSJL-12 through a morphological biochemistry and molecular biology method, and a preservation number is CGMCC No. 9062. The bacterial strain inhibits Listeria monocytogenes CICC21633 to positive. The prepared solid probiotics contain soybean meal and active Lactobacillus reuteri YSJL-12, can substitute aureomycin for preventing and treating diarrhoea while added in a live pig basic feed, and the feed-gain ratio of the breeding pork pig at age in days of 28-168 is 2.61. The bacterial strain has the characteristics of strong host specificity, large live bacteria quantity, low cost and high cost performance, added value of high temperature soybean meal is increased, and the green live pig culture cost is reduced.

The invention relates to penicillium oxalicum LJ302 capable of degrading chlortetracycline, and belongs to the technical field of microbes. The penicillium oxalicum LJ302 is named, and is preserved in General Microorganism Centre of China Microbiological Preservation and Management Committee in Dec 19, 2012 with an accession number of CGMCC No. 7019. The rDNA-ITS sequence of the LJ302 bacterial strain is composed of 572 bases. The LJ302 bacterial strain can grow in a medium with chlortetracycline as a sole carbon source, and has a good degradation effect on chlortetracycline remained in wastes.

The invention belongs to the technical field of drug preparation, particularly relates to a clathrate compound of an aureomycin zinc complex, and a preparation method thereof, and aims to solve the technical problem that the aureomycin zinc complex is poor in solubility and small in dissolution rate. Through the technical scheme, the clathrate compound of the aureomycin zinc complex comprises a clathrate material and the aureomycin zinc complex according to a weight ratio of (1-3): 1, and the clathrate material is cyclodextrin or a cyclodextrin derivative. The invention further provides an oral preparation prepared from the clathrate compound. The invention further provides a preparation method for the clathrate compound of the aureomycin zinc complex. The aureomycin zinc complex in the clathrate compound is good in solubility in water and high in dissolution rate, and has the advantages of good water solubility, high stability, good curative effect, little side effect and the like.

The invention relates to semi-artificial feedstuff for sesamie inferens and a preparation method of the feedstuff, belonging to artificial feedstuff for insects and feedstuff preparation technologies. The semi-artificial feedstuff is characterized in that each kilogram of feedstuff contains 21-27 grams of soybean flour, 25-60 grams of water bamboo flour, 18-36 grams of rice stem powder, 22-30 grams of corn flour, 16-22 grams of malt flour, 10-20 grams of rice sprout powder, 35-70 grams of yeast, 10-14 grams of cane sugar, 20-30 grams of casein, 1.5-2.8 grams of vitamin C, 0.05-0.15 gram of cholesterol, 5-8 grams of Wesson salt mixture, 2-4 grams of sorbic acid, 1.5-3.0 grams of chlortetracycline, 12-13 grams of agar, and the balance of distilled water. When the semi-artificial feedstuff is used to breed newly hatched larvae of sesamie inferens, the survival rate of the larvae of 12 days is more than 92%, the proportion of the larvae growing to pupas is more than 85%, and 10 generations of larvae can be continuously raised; during the breeding of each generation of larvae, the feedstuff only needs to be replaced once, thus simplifying the operation; and the raw materials are readily available and low in cost.

The invention discloses a method for diagnosing soil contamination by tetracycline antibiotics. The method includes the following steps: placing plant seeds in a group of soil with the antibiotic concentration being in gradient distribution for cultivation, after three days of cultivation, detecting the length of the root systems of the plant seeds and calculating the average value of the root length; establishing a relation curve between the antibiotics concentration in the soil and the average value of the root length; performing pretreatment on the to-be-detected soil containing antibiotics, placing plant seeds in the to-be-detected soil for cultivation, after three days of cultivation, detecting the length of the root systems of the plant seeds, and calculating the concentration of antibiotics in the to-be-detected soil according to the relation curve, wherein the plant seeds are Zaoshu No.8, the soil is blue purple clay soil, antibiotics are oxytetracycline and chlortetracycline, and the gradient range of antibiotic concentration in the soil is 0-5 mg.kg<-1>. The method provided by the invention can reflect the concentration of antibiotics in the to-be-detected sample, and has the characteristics of being simple, fast and low in cost, and the limitations of the traditional antibiotics detection method are overcome.

The invention relates to a method for preparing an organic fertilizer from chlortetracycline residues. The method comprises the following steps: enzymolysis separation: adding a reaction enzyme into chlortetracycline residue paste, adjusting a pH value to 4-7 with an inorganic base solution after uniform mixing, and carrying out solid-liquid separation after the mixture is kept in a static state for 1-2 hours at a temperature of 20-30 DEG C to obtain chlortetracycline residues subjected to enzymolysis; fermentation: adding rice bran into the chlortetracycline residues subjected to enzymolysis,mixing uniformly, adding fermented compound bacteria, carrying out fermentation at a temperature of 20-70 DEG C, turning over the mixture every 2-4 days during the fermentation, and carrying out drying, sieving and granulation after the fermentation continues for 10-30 days to obtain the organic fertilizer. According to the organic fertilizer prepared with the technique, the organic content (on adry basis) is equal to or larger than 70.0%, the total nutrient content (N+P2O5+K2O, on a dry basis) is equal to or larger than 7.0%, and no chlortetracycline is detected.

The present invention discloses an ELIA kit applicable to aureomycin recidue analysis and its application. Said kit includes kit body, enzyme-labelled plate and reagent, in which said reagent includes washing solution, sample diluent, horseradish peroxidase labeled antibody, substrate solution, color developing solution and stop buffer. The invented kernel lies in that it has the coated antigen, which is coated by coating solution and can be specifically reacted with aureomycin antibody, aureomycin standard solution and aureomycin antibody. Said antibody is the blood serum that is obtained by immunizing rabbit by utilizing artificial immune source, the coated antigen is the compound formed from aureomycin acetic acid and egg-white albumin, and the artificial immune source is the compound composed of aureomycin acetic acid and bovine serum albumin.

The invention discloses a chemiluminescence detection kit of aureomycin and a preparation method thereof. The kit comprises the following components: an acridinium ester labelled aureomycin antigen orantibody, a magnetic particle coupled antibody or antigen, an aureomycin series standard substance solution, chemiluminescence preexcitation liquid A, chemiluminescence preexcitation liquid B, and cleaning fluid. The kit combines chemiluminescence technology and immunomagnetic particles, and provides a reaction system approaching to a homogeneous phase. Compared with the prior art, the kit has the advantages of high sensitivity, good specificity, short reaction time, and the like.

The invention discloses a detection method for antibiotic residual in livestock meat. The detection method comprises the following steps: cleaning the livestock meat, blowing moisture on a surface to be dry, and then, cutting the livestock meat into slices of which the thickness is about 0.1-1.0cm for standby; spraying solid weak base on the slices or spraying weak base aqueous solution on the slices; putting the slices in a drying oven to be dried at the temperature of 30-60 DEG C, taking out the slices when the moisture content is 30-50%, washing in distilled water for 1-3 times, and then, continuously drying until the moisture content of the slices is not higher than 10%; grinding the slices, adding extractant for extraction, carrying out centrifugation, and taking supernate; after the supernate passes through a microfiltration membrane, adopting a ultra-high performance liquid chromatography and mass spectrum combined method to measure the antibiotic residual, wherein the antibiotics can be oxytetracycline, tetracycline, doxycycline and aureomycin. The detection method is characterized in that the influence of high protein muscle tissues on an antibiotic recovery rate can be avoided especially in the early-stage treatment of a sample, and detection accuracy is high.

The invention discloses acinetobacter baumannii for degrading aureomycin and application of the acinetobacter baumannii. The strain is named as (Acinetobacter baumannii)F3 with a preservation number of GDMCC NO:60375 and is preserved in Guangdong Microbial Culture Collection Center in Floor 5th, Building 59th, Courtyard 100th, Xianlie Middle Road, Guangzhou city, Guangdong province on on May 23, 2018. The acinetobacter baumannii can be used for degrading aureomycin in a co-metabolism manner, and the degradation rate of the acinetobacter baumannii to aureomycin with a low concentration of 10mg/L can reach 95.57% within 20h and can reach 99.04% within 35h; and meanwhile, the strain further has potassium dissolving performance and is beneficial to the improvement of soil fertility. Therefore,the acinetobacter baumannii can be used for removing residual aureomycin in liquid and soil and further has the efficacies of increasing potassium nutrient in soil and improving the soil fertility.