10 results about "Chlortetracycline" patented technology

The invention provides a ratio fluorescent probe for detecting aureomycin in meat food and a detection method. After an aureomycin solution is added into the prepared Au NCs ratiometric fluorescent probe solution, a fluorescence emission band of a reaction system at 620 nm is almost unchanged, and a fluorescence emission band at 425 nm is remarkably enhanced. Meanwhile, the color of a reaction system under an ultraviolet lamp is gradually changed from red to purple along with the increase of the concentration of the aureomycin, and quantitative detection of the aureomycin can be realized by utilizing the characteristics. The detection method disclosed by the invention comprises two detection methods, one is a ratio fluorescent probe detection method, and quantitative detection is realized by utilizing linear correlation between the F425 / F620 fluorescence intensity ratio and the chlortetracycline concentration. The other method is a visual quantitative detection method, a fluorescent paper chip image is collected by using a smart phone and converted into a corresponding RGB value, and quantitative detection is realized by using a linear relationship between an R / B value and the chlortetracycline concentration. The detection method disclosed by the invention has the advantages of high selectivity, high sensitivity, simplicity in operation, obvious phenomenon and good stability and repeatability.

The invention relates to an application of an aureomycin A derivative in preparation of an anti-tumor drug, the anti-tumor drug is a drug for preventing or treating liver cancer, and the aureomycin A derivative is selected from a compound shown as a formula (I), or a stereoisomer, a prodrug, a crystal form, a pharmaceutically acceptable salt, a pharmaceutically acceptable ester or a pharmaceutically acceptable solvate of the compound. The aureomycin A derivative disclosed by the invention can be used for remarkably inhibiting the proliferation of liver cancer cells Hepa1-6, and the half inhibitory concentration (IC50 value) is 0.4821 mu g / mL and 0.1701 mu g / mL respectively; 11 days after administration, the tumor volume of a Hepa1-6 ectopic transplantation C57BL / 6 mouse is reduced, and the tumor weight of a Hepa1-6 tumor ectopic transplantation tumor is reduced; the aureomycin A derivative has the effects of remarkably inhibiting proliferation of liver cancer cells at the cellular level and remarkably reducing tumor growth of the liver cancer cells at the animal level, and a research direction is provided for development of new drugs.

This invention discloses a dual-emission molecularly imprinted ratiometric fluorescence sensor, its preparation method, and its application in the detection of chlortetracycline, belonging to the field of chemical sensor technology. The preparation method of this sensor includes the following steps: preparing silica-quantum dot composite nanoparticles; preparing a molecularly imprinted polymer using salicylamide as a template molecule; and using a post-mixing strategy to mix the molecularly imprinted polymer and silica-quantum dot composite nanoparticles in HEPES buffer to construct the dual-emission molecularly imprinted ratiometric fluorescence sensor. The advantages of this invention are: the dual-emission molecularly imprinted ratiometric fluorescence sensor provided by this invention exhibits rich fluorescence color changes, enabling rapid, highly sensitive, and visual detection of chlortetracycline; and the entire detection process is simple, convenient, and fast, making it suitable for widespread application in practical applications.

The invention belongs to the technical field of feed additives, and particularly relates to a preparation method of sheep Zhuang powder, which comprises the following specific steps: preparing an astragalus extract, preparing berberine hydrochloride, and mixing for preparation. In the preparation process of the sheep strengthening powder, the feed additive is added, the feed additive effectively replaces the previous aureomycin, the antibacterial effect (MIC) of the feed additive on escherichia coli AZ1, escherichia coli 8-1 and salmonella sk77 is obviously better than that of the aureomycin, the dosage every time is smaller, animals are healthier, both pathogenic bacteria are killed, the immunity is improved, and the sheep strengthening powder is suitable for being used for preparing sheep strengthening powder. The feed is safe and compliant, has no residue and no withdrawal period, and has the effects of fattening and weight gaining and fierce eating and fierce growth.

The present invention relates to a magnetized modified polypropylene meltblown cloth activation material and a preparation method thereof. The prepared magnetized modified polypropylene meltblown cloth can effectively solve the agglomeration problem of magnetic ferrites in Fenton reactions such as sulfate radical advanced oxidation processes (SR-AOPs), and can activate persulfate in the catalytic system. Degradation and removal experiments have shown that the material has a good removal effect on the antibiotic chlortetracycline. Moreover, the material can relatively easily perform solid-liquid separation from contaminated water bodies under the action of an external magnetic field. The polypropylene meltblown cloth material used is extracted from discarded protective masks, which not only realizes the reuse of waste but also achieves the purpose of using waste to treat pollution.

The invention belongs to the technical field of aureomycin detection, and particularly relates to a preparation method of a carbon quantum dot ratiometric fluorescent probe and a method for detecting aureomycin. The method is based on the characteristic that aureomycin can enable a carbon quantum dot ratio fluorescent probe to correspondingly detect that the color of a system gradually changes from yellow to yellow green to dark blue to light purple to final dark purple under an ultraviolet lamp, and obvious changes occur. Detecting the fluorescence intensity change R425 / R515 of a mixed solution of a to-be-detected sample and D-CDs, and comparing with the standard curve to obtain the aureomycin content in the to-be-detected sample. Besides, a paper-based sensor containing the carbon quantum dot ratio fluorescent probe is prepared, a smart phone is used as a signal reader, carrying is convenient, on-site detection of aureomycin in a limited-resource environment can be achieved, and the detection method is simple, sensitive and efficient.

The present invention provides a method and application for preparing a Co3O4 / CuCo2O4 composite material based on Co-MOF materials, belonging to the technical field of material synthesis and analytical detection. The method comprises the following steps: S1, preparing a Co-MOF material; S2, preparing a Co3O4 / CuCo2O4 material precursor; S3, preparing a Co3O4 / CuCo2O4 composite material; the composite material is obtained by combining a novel Co-MOF material having a two-dimensional wavy layered structure with a copper salt and then calcining it to obtain a novel Co3O4 / CuCo2O4 composite material; the Co3O4 / CuCo2O4 composite material prepared by the present invention has bimetallic properties, can degrade tetracycline antibiotics and some dyes, has stable chemical properties, excellent performance in catalytic degradation of chlortetracycline, and has anti-interference ability.

This invention discloses a hindered Lewis acid-base pair type copper pyrovanadate catalyst, its preparation method, and its application. The preparation method of the catalyst includes the following steps: adding a metavanadate solution to a copper salt solution under stirring conditions, continuing stirring after complete addition to ensure sufficient reaction, then transferring the resulting mixture to a reaction vessel for hydrothermal reaction, and after the reaction is completed, centrifuging to separate the solid, washing, and drying to obtain the hindered Lewis acid-base pair type copper pyrovanadate catalyst. This catalyst can promote singlet oxygen (… 1 The generation of O2 can be used to activate oxidants such as persulfate (PMS) to degrade organic pollutants in water. These organic pollutants include antibiotics, specifically at least one of tetracycline (TC), oxytetracycline (OTC), chlortetracycline (CTC), doxycycline (DOX), and their derivatives. The preparation method of this invention is simple, can be mass-produced, and is inexpensive, thus possessing broad application prospects.

The invention discloses a method for efficiently purifying aureomycin A in a streptomyces fermentation product, which only needs to perform drying, ultrasonic extraction, concentration and crystallization on the fermentation product, then dissolve the fermentation product with an organic solvent and then crystallize the fermentation product, and is simple to operate, low in equipment requirement, low in consumable cost, convenient in organic solvent recovery and low in cost. The problems of complex separation and purification steps, high equipment requirements, long time consumption and the like required by liquid-phase preparative chromatography in the existing method are solved. According to the invention, after the first crystallization and washing are completed, the purity of the aureomycin A in the obtained aureomycin A crude product is 87-92%. After recrystallization and secondary washing of the aureomycin A crude product, the purity of the aureomycin A crude product reaches 95% or above, the total yield reaches 47% or above, the total consumed time is 90-144 h, and the aureomycin A crude product is suitable for large-scale preparation, low in cost and high in benefit.

The invention belongs to the technical field of fluorescence analysis, and discloses a preparation method and application of a fluorescence closing probe for detecting CTC. H2SDC, anhydrous AlCl3 and glacial acetic acid react at a certain temperature, and the fluorescence closed probe CYCU-3 is obtained through the steps of centrifugal separation, washing, drying and the like and is used for detecting the concentration of chlortetracycline (CTC). According to the present invention, 4, 4 '-stilbene dicarboxylic acid (H2SDC) is adopted as the raw material to synthesize the aluminum-based fluorescence closing probe, such that CTC detection is achieved, and the detection limit is extremely low (45.551 nM);