Penicillium oxalicum LJ302 capable of degrading chlortetracycline

A technology of LJ302 and Penicillium oxalicum, which is applied in the field of microorganisms, can solve the problems of less aureomycin waste and no reports, etc., and achieve the effects of good degradation, wide range of suitable growth and good adaptability

Inactive Publication Date: 2013-08-28
BEIJING INSTITUTE OF TECHNOLOGYGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are very few research reports on the screening of aureomycin-degrading strains and the use of efficient strains to degrade aureomycin waste at home and abroad.
And the research on penicillium oxalicum degrading aureomycin aspect, there is no report both at home and abroad

Method used

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  • Penicillium oxalicum LJ302 capable of degrading chlortetracycline
  • Penicillium oxalicum LJ302 capable of degrading chlortetracycline
  • Penicillium oxalicum LJ302 capable of degrading chlortetracycline

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Screening method for a strain of Penicillium oxalicum LJ302 degrading aureomycin

[0031] 1.1 Material preparation

[0032] Source of bacteria samples: collected from the waste of aureomycin production enterprises.

[0033] Inorganic salt solid medium: (NH4) 2 SO 4 2.00g, K 2 HPO 4 0.50g, KH 2 PO 4 0.50g, MgSO 4 7H2O0.50g, NaCl0.20g, CaCl 2 0.1g, FeSO 4 0.01g, EDTA0.015g, agar 13.00g, glucose 2.00g / L, dissolved in 1000ml distilled water. After sterilizing at 115°C for 20 minutes, add 2.00 g / L aureomycin to the ultra-clean workbench.

[0034] Martin's medium: peptone 6.0g, glucose 10.0g, KH 2 PO 4 1.0g, MgSO 4 ·7H2O0.5g, dissolved in 1000ml of distilled water. Sterilize at 115°C for 20 minutes.

[0035] Select medium: (NH4) 2 SO 4 2.00,K 2 HPO 4 0.50, KH 2 PO 4 0.50, MgSO 4 7H2O0.50, NaCl0.20, CaCl 2 0.10, FeSO 4 0.01g, EDTA0.015g, dissolved in 1000ml distilled water. Sterilize at 115°C for 20 minutes. After cooling, add 0.1g / L, 1.0g / L, 2.0g / ...

Embodiment 2

[0073] Growth and degradation experiments of strain LJ302 in aureomycin as the only carbon source medium:

[0074] Prepare inorganic salt solid medium with chlortetracycline as the only carbon source and the concentrations of chlortetracycline are 0.1g / L, 1.0g / L, 5.0g / L, 10.0g / L and 15.0g / L respectively. Pick a few LJ302 bacterium colonies from the activated culture medium, draw a line on the above-mentioned inorganic salt solid medium plate, and culture in the dark. It is known through observation that the bacterial strain LJ302 has a concentration of chlortetracycline of 0.1g / L, 1.0g / L and 5.0g / L plates grew mycelia. It shows that the strain LJ302 can grow in the medium whose concentration of aureomycin is 0.1g / L to 5.0g / L.

[0075] Insert the activated strain LJ302 into the inorganic salt liquid medium with chlortetracycline as the sole carbon source and the content of chlortetracycline as 500 mg / L, culture it in a shaker at 28°C and 170 r / min, and take samples regularly t...

Embodiment 3

[0077] Experiment of strain LJ302 on the treatment of chlortetracycline wastewater:

[0078] The strain LJ302 was activated and cultured with Martin's medium, and then added to the production wastewater of Chlortetracycline Enterprise according to the inoculum amount of 1%, and the original wastewater without microorganisms was used as a control, and placed in a shaker at 28°C and 170r / min respectively. Cultivate in the bed for 2 days, and determine the residual amount of aureomycin in the waste water. It was determined that the removal rate of aureomycin by the inoculated strain LJ302 was 25.64% higher than that of the control.

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Abstract

The invention relates to penicillium oxalicum LJ302 capable of degrading chlortetracycline, and belongs to the technical field of microbes. The penicillium oxalicum LJ302 is named, and is preserved in General Microorganism Centre of China Microbiological Preservation and Management Committee in Dec 19, 2012 with an accession number of CGMCC No. 7019. The rDNA-ITS sequence of the LJ302 bacterial strain is composed of 572 bases. The LJ302 bacterial strain can grow in a medium with chlortetracycline as a sole carbon source, and has a good degradation effect on chlortetracycline remained in wastes.

Description

technical field [0001] The invention relates to a strain of Penicillium oxalicum LJ302 capable of degrading aureomycin, belonging to the technical field of microorganisms. Background technique [0002] Chlortetracycline is a tetracycline drug, mainly produced in my country, which belongs to a broad-spectrum antibiotic, mainly inhibits the protein synthesis of sensitive microorganisms, and is effective against Gram-positive bacteria, Gram-negative bacteria, spirochetes, rickettsia, and mycoplasma , Chlamydia, and some protozoa have inhibitory effects, and have a wide range of uses in medicine, animal husbandry and other fields. [0003] During the fermentation production process of chlortetracycline, a large amount of bacterial residue and waste water containing chlortetracycline residue will be produced. If it is not properly treated and enters the environment, it will cause potential harm to the ecological environment and human health. At present, the biological detoxificat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A62D3/02C02F3/34C12R1/80A62D101/28C02F101/38
Inventor 李艳菊杨正礼肖思颖
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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