4239 results about "Microbiological Techniques" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to a Prlisteria ataveovii strain and application thereof. The invention provides a Pichia aryabhattai B8, which is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms, the preservation date is September 10, 2024, and the preservation number is CGMCC No.31932. The Pichia aryabhattai B8 disclosed by the invention has the advantages that the Pichia aryabhattai B8 can be used for preparing the Pichia aryabhattai B8; the prelisteria aryabhattai B8 disclosed by the invention has a relatively strong function of producing indoleacetic acid, and the content of indoleacetic acid in plants can be increased, so that the stress resistance of the plants is improved; dissolution of insoluble phosphorus and potassium in soil can be promoted, soil nutrients are improved, plant growth is promoted, and plant quality is improved; and meanwhile, the P.aryabhattai B8 has a certain antagonistic effect on pathogenic bacteria of the wheat root rot, and can be used for preventing and treating the wheat root rot.

The invention belongs to the technical field of microorganisms, and particularly relates to recombinant escherichia coli containing a PDRN sequence and a preparation method thereof, and the preparation method comprises the following steps: S1, preparing a PDRN solution; s2, complementing the double strands of the PDRN into blunt ends; s3, constructing a recombinant plasmid; s4, carrying out mixed culture on a product obtained in the step S3 and escherichia coli DH5alpha; s5, screening the recombinants; s6, carrying out PCR (Polymerase Chain Reaction) amplification, and recording a strain from a fragment with the length of more than 200bp; s7, preparing a seed solution by adopting the strain prepared in the step S6; s8, strain fermentation; and S9, centrifugally collecting thalli. The method comprises the following steps: constructing a recombinant plasmid containing a PDRN fragment, introducing the recombinant plasmid into Escherichia coli, subsequently culturing the recombinant Escherichia coli to copy DNA in the Escherichia coli, extracting a purified exogenous gene fragment to obtain a strain containing a single fragment PDRN sequence, and getting rid of dependence on raw materials during subsequent extraction.

The invention discloses bacillus velezensis as well as fermentation product filtrate and a biological product thereof, and belongs to the technical field of microorganisms, a bacillus velezensis BTN-HB-F4 strain is separated and purified from prinsepia utilis royle in Harba snow mountain in Yunnan province, the fermentation product filtrate of the bacillus velezensis has excellent HAS inhibition capacity and remarkable DPPH and ABTS free radical scavenging capacity, and the biological product of the bacillus velezensis has the advantages that the biological product can be used for preparing biological products of the prinsepia utilis royle; a good anti-oxidation effect can be realized; secondly, under various concentrations, fermentation product filtrate can remarkably reduce the proportion of SA-beta-Gal positive cells, gene expression of matrix metalloproteinase 3 can be remarkably inhibited, and a good anti-aging effect is achieved; in addition, fermentation product filtrate can remarkably inhibit generation of zebra fish melanin and has a good whitening effect; the secretion amount of cell inflammatory factors IL-6 and TNF-alpha can be remarkably inhibited, and a good relieving effect is achieved; finally, the fermentation product filtrate can effectively inhibit the growth of pathogenic bacteria staphylococcus aureus, and has the potential of regulating the skin micro-ecology.

The invention relates to the technical field of microorganisms, and provides a low-temperature-resistant heterotrophic nitrification-aerobic denitrification pseudomonas and application thereof, the strain is pseudomonas (Pseudomonas sp.) SW1, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 20221797. The low-temperature-resistant heterotrophic nitrification-aerobic denitrification bacterium Pseudomonas sp. SW1 has the characteristic of low temperature resistance, can well grow at 4-10 DEG C, and has relatively strong heterotrophic nitrification-aerobic denitrification nitrogen removal capability, and the enrichment culture of the strain is carried out in a relatively low nitrogen source, and the condition is extremely consistent with the application scene of the strain in domestic sewage.

The invention discloses lactobacillus kefiranofaciens IMAU12319 and application thereof, belongs to the technical field of microorganisms, and particularly relates to lactobacillus kefiranofaciens IMAU12319 which is preserved in China General Microbiological Culture Collection Center (CGMCC) on July 8, 2024, the preservation address is No. 3, No.1 yard, West Beichen Road, Chaoyang District, Beijing, and the number is CGMCC No.31224. The lactobacillus kefiranofaciens IMAU12319 has the advantages that the lactobacillus kefiranofaciens IMAU12319 can be used for preparing the lactobacillus kefiranofaciens IMAU12319, and the lactobacillus kefiranofaciens IMAU12319 can be used for preparing the lactobacillus kefiranofaciens IMAU12319; the invention also relates to an application of the lactobacillus kefiranofaciens IMAU12319 in preparation of a probiotic agent capable of resisting gastrointestinal digestion. The strain disclosed by the invention not only shows good gastrointestinal tract endurance capacity, but also shows relatively strong potential in the aspects of intestinal adhesion, oxidation resistance and bacteriostasis, and has a wide application prospect in leavening agent strains.

The invention discloses bacillus cereus and application thereof, and belongs to the technical field of microorganisms. The bacillus cereus SNF7 is separated from a saline-alkali soil sample, has the functions of resisting salt, fixing nitrogen and promoting growth, has an obvious inhibition effect on some common soil-borne diseases in agriculture, such as pathogenic bacteria of peanut fruit rot, tomato root rot and the like, has multiple functions of resisting salt, fixing nitrogen, biologically preventing and controlling and the like, and has a wide application prospect. The method can be widely applied to crop planting in large saline-alkali soil in China.

The invention belongs to the technical field of microorganisms, particularly relates to rice endophytic bacillus velezensis AN6 and application thereof, and particularly provides bacillus velezensis AN6 which is classified and named as bacillus velezensis and has the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20231886. The invention provides a novel rice endophytic bacillus velezensis strain AN6, through separation, screening, identification and experimental verification, it is found that the strain shows broad-spectrum resistance to various phytopathogens including rice bacterial leaf blight, rice blast and the like, the strain has the capacity of inhibiting various phytopathogens and has the capacity of adsorbing heavy metal, and the strain has the advantages of being capable of inhibiting various phytopathogens and capable of being used as a novel rice endophytic bacillus velezensis strain AN6. The method is of great significance to biological control of rice diseases and development of green agriculture.

The invention relates to lactobacillus rhamnosus CBBM005 and application thereof in products for balancing intestinal flora, improving immunity and improving oral cavity, and belongs to the technical field of microorganisms. The lactobacillus rhamnosus CBBM005 is separated from human intestinal secretion, the lactobacillus rhamnosus CBBM005 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation time is March 20, 2025, and the preservation number is CGMCC NO.33904. The lactobacillus rhamnosus CBBM005 has the characteristics of balancing intestinal flora, improving immunity and improving oral cavity.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus mobilis DG-7 and application thereof in inhibiting bolting of angelica sinensis and improving disease resistance of angelica sinensis. The invention provides a strain of bacillus mobilis DG-7, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.31089. The bacillus mobilis DG-7 and the microbial inoculum thereof provided by the invention can play roles in inhibiting early bolting of angelica sinensis and improving disease resistance by increasing the content of plant endogenous melatonin and salicylic acid, reducing the content of abscisic acid, auxin and gibberellin, reducing the content of total phenolic acid and reducing the expression of photoperiod-related genes. In addition, the bacillus mobilis DG-7 does not solubilize phosphorus, so that the promotion effect of high-concentration phosphorus on early bolting is effectively avoided; the laccase activity of the bacillus mobilis DG-7 is high, polyphenol substances can be effectively removed, and the bolting rate can be reduced.

The invention relates to the technical field of microorganisms, and in particular relates to application of a strain of lactobacillus mucus TG018. The invention further relates to a preparation method of the lactobacillus mucus TG018. The preservation number of the lactobacillus mucus TG018 is CCTCC (China Center for Type Culture Collection) No: M 2023514; the lactobacillus mucus TG018 can be used for producing nicotinic acid, serving as an antioxidant and / or preparing the antioxidant, or can be applied to medicines for reducing uric acid.

The invention discloses aureobasidium pullulans, application thereof and a method for producing medium-molecular-weight pullulan, and belongs to the technical field of microorganisms. The aureobasidium pullulans disclosed by the invention is aureobasidium pullulans SYCY-018, the strain is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on December 26, 2022, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.40467. According to the aureobasidium pullulans SYCY-018, the aureobasidium pullulans SYCY-018 and the aureobasidium pullulans The aureobasidium pullulans SYCY-018 is inoculated into a fermentation medium for fermentation to produce the pullulan, the yield of the medium-molecular-weight pullulan reaches up to 150 g / L or above, meanwhile, good hereditary stability and high temperature resistance are achieved, the yield of the pullulan can still be higher than 130 g / L even at the high temperature of 40 DEG C, and the aureobasidium pullulans SYCY-018 is suitable for industrial production.

The invention relates to bacillus velezensis and application thereof in preparation of a water-soluble microbial agent, and belongs to the technical field of microorganisms. The bacillus velezensis has the effects of producing exopolysaccharides, promoting the formation of soil aggregates, degrading abamectin, hydrolyzing protein, producing indoleacetic acid, promoting the growth of tomato plants and promoting the enzyme activity of tomato rhizosphere soil enzyme. The fertilizer not only has the effects of improving the soil aggregate structure and promoting the growth of tomato plants, but also can accelerate the temperature rise of the abamectin fermentation waste residue compost, prolong the high-temperature period and shorten the compost period, and is beneficial to promoting the harmless treatment and resource utilization of the abamectin fermentation waste residue.

The invention relates to bacillus velezensis and application thereof in preparation of a multifunctional straw decomposition agent, and belongs to the technical field of microorganisms. The bacillus velezensis has the functions of producing cellulase, producing xylanase, degrading abamectin, decomposing inorganic phosphorus and degrading straws; when straw is returned to the field, the multifunctional straw decomposition agent provided by the invention not only can accelerate the decomposition speed of straw, decompose abamectin residues in soil and improve the soil ecology, but also can promote the growth of pepper plants and enhance the stress resistance of the pepper plants, and has a good comprehensive application effect.

The invention relates to the technical field of microorganisms, in particular to application of Cladosporium LC3 in crop growth promotion and bacterial wilt resistance, the strain LC3 belongs to Cladosporium and Cladosporium immonda species, the strain is separated from Guangxi sugarcane rhizosphere soil, experimental evaluation screening finds that the strain has a good growth promotion function on tomatoes and fresh ginger, and the strain can be applied to crop growth promotion and bacterial wilt resistance. The yield of ginger is remarkably increased, and the growth vigor of tomato plants is effectively improved; meanwhile, the strain has a good prevention and treatment effect on tomato and ginger bacterial wilt and has good plant bacterial wilt resistance, and screening verification of the strain provides a new fungicide and a raw material for later-period growth of an anti-microbial agent or a biological organic fertilizer.

The invention relates to the technical field of microorganisms, in particular to a viable bacterium preparation of a lactobacillus paracasei strain as well as a preparation method and application of the viable bacterium preparation. The viable bacterium preparation comprises a lactobacillus paracasei strain and auxiliary materials, and the preservation number of the lactobacillus paracasei strain is CGMCC (China General Microbiological Culture Collection Center) No.27974. The viable bacterium preparation can inhibit the growth of streptococcus mutans, reduce the formation of a dental plaque biofilm caused by the streptococcus mutans, reduce the content of a mature dental plaque biofilm, inhibit the level of water-insoluble exopolysaccharides generated by the streptococcus mutans, reduce the expression quantity of cariogenic genes gtfB, gtfC, gtfD, ftf and LuxS of the streptococcus mutans, improve the cariogenic activity of the streptococcus mutans and improve the cariogenic activity of the streptococcus mutans. The adhesion rate of streptococcus mutans on a hydroxyapatite tooth model is reduced, and a beneficial in-vitro probiotic function is achieved.

The invention discloses construction and application of an L-malic acid producing issatchenkia orientalis strain, and belongs to the technical field of microorganisms and the technical field of microbial fermentation and metabolic engineering. Through a metabolic engineering means, a cytoplasm metabolic pathway of an issatchenkia orientalis strain is transformed, and a pyruvate decarboxylase coding gene, glycerol dehydrogenase and lactic dehydrogenase are knocked out; a pyruvate carboxylase gene derived from aspergillus flavus, a malate dehydrogenase gene derived from corynebacterium glutamicum and phosphoenolpyruvate carboxylase derived from escherichia coli are screened and integrated, so that a cytoplasm rTCA cycle synthesis pathway is enhanced; inserting a dicarboxylic acid external transporter gene from schizosaccharomyces cerevisiae at a neutral site GD21, and knocking out endogenous dicarboxylic acid internal transporter; the L-malic acid yield of the modified yeast strain in the fermentation process is remarkably increased and reaches 140.16 g / L. The engineering strain provided by the invention has good industrial application potential in efficient L-malic acid production.

The invention discloses a salt-tolerant zygosaccharomyces rouxii strain with high ethanol yield and application thereof in preparation of soy sauce, and belongs to the technical field of microorganisms, the salt-tolerant zygosaccharomyces rouxii strain SS-y178 is preserved in the China Center for Type Culture Collection on July 15, 2024, the preservation number is CCTCC NO: M 20241563, the preservation address is Wuhan University, Wuhan, China, the salt-tolerant zygosaccharomyces rouxii strain is applied to fermentation production of soy sauce, and the salt-tolerant zygosaccharomyces rouxii strain can be used for preparing the soy sauce. The method has the advantages of high yield of ethanol, promotion of fermentation fat production and increase of the types of lipid substances, has good effects in promotion of fat increase and aroma enhancement of soy sauce, and has wide application prospects.

The invention relates to the technical field of environmental microorganisms, in particular to high-temperature-resistant bacillus subtilis and application thereof. The invention discloses a high-temperature-resistant bacillus, which is named as bacillus subtilis HY-1, has been preserved in China General Microbiological Culture Collection Center (CGMCC) on October 17, 2024, and has a preservation number of CGMCC No.32250. The invention further discloses a preparation method of the high-temperature-resistant bacillus. The invention further discloses a culture method of the bacillus, a liquid fungicide prepared from the bacillus and application of the bacillus in kitchen waste biogas residue compost and straw degradation. The high-temperature-resistant bacillus disclosed by the invention is derived from a compost indigenous strain, can reduce the inhibition effect of other indigenous bacteria, and has relatively good degradation capability and high-temperature-resistant performance on cellulose. The method has a wide application prospect in the field of kitchen waste biogas residue resourceful treatment.

The invention discloses two phosphate solubilizing bacteria and application of microbial agents thereof, and belongs to the technical field of microorganisms. The preservation number of the bacillus cereus Sau.XJ-4 is CCTCC (China Center For Type Culture Collection) NO: M 20242890; the preservation number of the arthrobacter sp. Sau.XJ-5 is CCTCC (China Center for Type Culture Collection) NO: M 20242891. The bacillus cereus Sau.XJ-4 and the bacillus cereus Sau.XJ-5 have the characteristics of saline-alkali resistance, aluminum resistance and the like, insoluble inorganic mineral substances such as tricalcium phosphate can be effectively decomposed, insoluble nutrient elements such as nitrogen, potassium and phosphorus in soil are promoted to be converted into soluble nutrients, the content of available nutrients in the soil is increased, and the yield and the quality are improved; the fertilizer can also relieve aluminum stress of plants and promote growth and development of the plants, and has important significance for fully exerting soil ecological fertility and keeping balance of an agricultural ecological environment.

The invention relates to the technical field of microorganisms, and provides lactobacillus mucilaginosus for maintaining genital tract health and preventing genital tract infection and application of lactobacillus mucilaginosus. Fermented mucus lactobacillus for maintaining genital tract health and preventing genital tract infection is named as FMTA1113H and is preserved in China General Microbiological Culture Collection Center (CGMCC) on April 22, 2025, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.34291. The invention further discloses a preparation method of the fermented mucus lactobacillus for maintaining genital tract health and preventing genital tract infection. According to the technical scheme, the problems that female genital tracts are prone to infection and relapse is prone to occurring after infection in the prior art are solved.

The invention relates to the technical field of microorganisms, in particular to application of lactobacillus rhamnosus KF7 in improving intestinal barrier injury and regulating intestinal flora and functional food containing the lactobacillus rhamnosus KF7. The invention provides application of lactobacillus rhamnosus KF7 in preparation of a product for improving intestinal barrier injury and / or regulating intestinal flora. The preservation number of the lactobacillus rhamnosus KF7 is CGMCC (China General Microbiological Culture Collection Center) NO.6430. The invention further provides application of the lactobacillus rhamnosus KF7 in preparation of a product for improving intestinal barrier injury and / or regulating intestinal flora. The invention discloses the novel application of the caseus rhamnosus KF7 in the aspects of improving the normal intestinal epithelial barrier function, protecting the normal intestinal epithelial barrier from lipopolysaccharide induced barrier injury, recovering animal intestinal barrier injury caused by rhythm disorder, regulating intestinal flora disorder and improving growth, development and metabolic states for the first time. The method can be used for producing functional food products such as fermented milk, lactic acid bacteria beverages, probiotic powder, viable bacteria water, milk powder and cheese.

The invention discloses a high-efficiency lespedeza growth-promoting bacillus Q4 (Bacillus atrophaeus) capable of improving the abundance of dominant bacteria and a preparation method of the high-efficiency lespedeza growth-promoting bacillus Q4. Belongs to the technical field of microorganisms. According to the bacillus atrophaeus Q4 provided by the invention, the conversion from total-effect nutrients to effective nutrients is improved, the consumption of lespedeza to soil nutrients is enhanced, and the abundance of dominant strain groups is increased, so that the effect of promoting the growth of lespedeza is achieved. After the bacillus atrophaeus Q4 provided by the invention is applied, the soil enzyme activity is remarkably improved, the conversion of available nutrients in soil is remarkably enhanced, and the nutrient consumption capability of lespedeza is enhanced. By applying the bacillus atrophaeus Q4, growth and development of lespedeza are promoted, the content of soil hydrolytic nitrogen and available phosphorus is remarkably increased, conversion to soil nutrients is improved, and the conductivity and organic carbon are increased to the maximum extent and are remarkably increased by 142.77% and 64.26% respectively. In the genus level, after being treated by the bacillus atrophaeus Q4, the bacillus (Bacillus) is obviously increased from 0.84% to 2.47%, and the average relative abundance of the pseudomonas (Fictibacillus) is obviously increased from 0.59% to 1.16%. By inoculating the Q4 inoculant, the structure of a soil colony is greatly improved, the relative abundance of a dominant flora is influenced, and the obvious increase of the relative abundance of Bacillus and Fictibacillus is possibly an important factor for promoting plant growth.

The invention belongs to the technical field of microorganisms, and provides a growth-promoting P.adami M6 and application thereof, the P.adami M6 is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC No: M20242835, the preservation date is December 18, 2024, the preservation number is CCTCC No: M20242835, the preservation number is CCTCC No: M20242835, the preservation number is CCTCC No: M20242835, and the preservation number is CCTCC No: M20242835. The strain is separated from rhizosphere soil of field planting foxtail millet in the Inner Mongolia red peak area, and is obtained through artificial separation and purification; the strain has the growth promoting characteristics of nitrogen fixation, phosphorus dissolution, IAA production and siderophore production; the M6 fungicide is used for inoculating millet, so that the growth of millet seedlings can be obviously promoted, and the plant height, the root length and the overground part and underground part biomass of the millet are obviously increased; m6 is used as a microbial agent to soak seeds during sowing and irrigate a bacterial liquid once 7 days after sowing, so that the growth effect of millet is best.

The invention relates to the technical field of microorganisms, in particular to bacillus velezensis F0b and an application of the bacillus velezensis F0b. The bacillus velezensis F0b is preserved in the Guangdong Microbial Culture Collection Center on April 26, 2024, the preservation date of the bacillus velezensis F0b is April 26, 2024, and the preservation number of the bacillus velezensis F0b is GDMCC (China General Microbiological Culture Collection Center): 64566. The bacillus velezensis has prevention and / or control effects on pyricularia oryzae, cabbage sclerotinia sclerotiorum, tomato fusarium wilt, banana fusarium wilt, rice leaf spot, peronophythora litchii, soybean carbon rot, colletotrichum capsici and corn leaf curvularia, and can be used for preparing broad-spectrum microbial source bactericides. The fermentation liquor of the bacillus velezensis can generate amylase, carboxymethyl cellulose and protease, and the fermentation liquor of the bacillus velezensis has prevention and / or control effects on vegetable pathogenic fungal diseases, namely cabbage sclerotiniose or pepper anthracnose.

The present invention relates to the technical field of microorganisms. Disclosed are lacticaseibacillus paracasei and a postbiotic product prepared therefrom, and a use of both. The present invention provides lacticaseibacillus paracasei (Latin name) YJS05199, which has the preservation number of GDMCC No.63668, and was preserved in Guangdong Microbial Culture Collection Center on 19 July 2023. The present invention further provides a postbiotic prepared by applying the lacticaseibacillus paracasei. The lacticaseibacillus paracasei and the postbiotic prepared therefrom have a bacteriostatic effect, can inhibit the weight gain of a mouse fed with high-fat diets, reduce the adipose tissue weight and the blood glucose concentration, and can remarkably reduce the levels of TG, TC, and LDL-C in serum of the mouse. Thus, the lacticaseibacillus paracasei and the postbiotic prepared therefrom have great application prospects in preparation of products for preventing or treating obesity and related diseases or in preparation of bacteriostatic products.

The invention relates to a strain of Prussella reevesii GY-2 and application thereof, and belongs to the technical field of microorganisms, the Prussella reevesii GY-2 simultaneously has the capabilities of fixing nitrogen, degrading protein, degrading cellulose, degrading fat and producing siderophores, has a remarkable growth promoting effect on wheat in a saline-alkali environment, can promote the growth of cucumbers, and has a wide application prospect.

The invention relates to the technical field of microorganisms, in particular to application of streptococcus non-lactolyticus GX-7 and a complex microbial inoculant thereof in sugarcane leaf silage, the strain GX-7 is separated from healthy pig manure by a research group, and through detection, the strain has good cholate resistance and is suitable for surviving in intestinal tracts, and the strain GX-7 has good cholate resistance and is suitable for being used as a feed additive. The antibacterial effect on pseudomonas fluorescens, listeria monocytogenes, salmonella choleraesuis and shigella flexneri is achieved; the strain has a good fermentation capability on sugarcane leaves, and the sugarcane leaves are inoculated with a complex microbial inoculant prepared from streptococcus non-lactolyticus GX-7 and streptococcus non-lactolyticus GX-2, so that a synergistic fermentation effect can be achieved; after the silage prepared from the complex microbial inoculant is fed to female buffalo in the gestation period, it is found that the silage can effectively increase the content of milk protein, milk total solids and milk non-fat solids in colostrum of the female buffalo and relieve diarrhea of the female buffalo, and the silage is safe and good.

The invention belongs to the technical field of microorganisms, and relates to high-activity plant lactobacillus KL100 for relieving anxiety and depression and application of the high-activity plant lactobacillus KL100. The plant lactobacillus provided by the invention is KL100, is preserved in the China General Microbiological Culture Collection Center (CGMCC), has a preservation number of CGMCC No.27192, and is classified and named as the plant lactobacillus. The plant lactobacillus is KL100, has a good gastrointestinal passing rate, shows a breeding characteristic in the stomach after being embedded, is improved in cholate-resistant survival rate, and can be used for preparing the plant lactobacillus. According to the application disclosed by the invention, the expression of IL-1beta and TNF-alpha in a mouse body can be effectively reduced, the expression of IL-10 can be improved, and the secretion of 5-hydroxytryptamine by chromaffin cells in intestinal tracts can be promoted, so that anxiety and depression can be relieved, the strain resource is expanded, the development of the biological medicine field is promoted, and a new direction is provided for treating anxiety and depression.

The invention relates to pediococcus pentosaceus with a cholesterol lowering function and application thereof, and belongs to the technical field of microorganisms. The invention discloses pediococcus pentosaceus with a cholesterol lowering function and application of the pediococcus pentosaceus. The pediococcus pentosaceus R5 (GDMCC No.65659) is separated from special fermented food stinky acid in Guizhou. The in-vitro cholesterol removal rate of the pediococcus pentosaceus R5 can reach 59%, and the pediococcus pentosaceus R5 has the characteristics of acid / cholate resistance, no hemolysis and sensitivity to common antibiotics; an in-vivo lipid-lowering experiment is carried out on a hyperlipidemia model mouse, and the result shows that the strain R5 can remarkably improve the in-vivo dyslipidemia state of the mouse, reduce liver fat accumulation and protect the liver at the same time, and can be used for developing lipid-lowering related probiotic functional products. The strain R5 provided by the invention provides a natural and safe probiotic for preventing and / or treating hyperlipemia.

The invention belongs to the technical field of microorganisms, and particularly relates to a postbiotic preparation for pets as well as a preparation method and application of the postbiotic preparation. The metabolite preparation provided by the invention comprises a lactobacillus plantarum Ps-8 inactivated thallus and a metabolite thereof, a lactobacillus casei C-9 inactivated thallus and a metabolite thereof, a bifidobacterium animalis subsp. Lactis BB-16 inactivated thallus and a metabolite thereof, and a lactobacillus acidophilus LA-14 inactivated thallus and a metabolite thereof. The pet food can be applied to various food types of different varieties of cat and dog pets, and has the effects of improving the intestinal barrier function of the pets, enhancing the immunity, reducing the incidence rate of diarrhea, reducing the food change stress, promoting the digestion of nutrient substances, optimizing the state of spirit and fur, reducing the odor of excrement, fading the odor of the oral cavity, improving the health level of the oral cavity and teeth and the like.