5538 results about "Microbiological Techniques" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to a Prlisteria ataveovii strain and application thereof. The invention provides a Pichia aryabhattai B8, which is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms, the preservation date is September 10, 2024, and the preservation number is CGMCC No.31932. The Pichia aryabhattai B8 disclosed by the invention has the advantages that the Pichia aryabhattai B8 can be used for preparing the Pichia aryabhattai B8; the prelisteria aryabhattai B8 disclosed by the invention has a relatively strong function of producing indoleacetic acid, and the content of indoleacetic acid in plants can be increased, so that the stress resistance of the plants is improved; dissolution of insoluble phosphorus and potassium in soil can be promoted, soil nutrients are improved, plant growth is promoted, and plant quality is improved; and meanwhile, the P.aryabhattai B8 has a certain antagonistic effect on pathogenic bacteria of the wheat root rot, and can be used for preventing and treating the wheat root rot.

The invention relates to paenibacillus polymyxa FangSG106 as well as application, a product and a method thereof, and belongs to the technical field of microorganisms. The invention provides paenibacillus polymyxa FangSG106, the preservation number of the paenibacillus polymyxa FangSG106 is CGMCC (China General Microbiological Culture Collection Center) No.31655. The invention also provides application of the paenibacillus polymyxa FangSG106 with the preservation number of CGMCC No.31655 in the aspects of non-treatment-destination bacteriostasis, plant disease prevention and treatment and plant growth promotion, and based on the paenibacillus polymyxa FangSG106, the application of the paenibacillus polymyxa FangSG106 in the aspects of non-treatment-destination bacteriostasis, plant disease prevention and treatment and plant growth promotion is The invention provides a plant growth promoter, a biopesticide, a method for preventing and treating plant diseases, a method for promoting plant growth and a bacteriostasis method. The paenibacillus polymyxa FangSG106 disclosed by the invention has broad-spectrum antibacterial activity, and can be used for effectively preventing and treating plant diseases and promoting plant growth.

The invention belongs to the technical field of microorganisms, and particularly relates to recombinant escherichia coli containing a PDRN sequence and a preparation method thereof, and the preparation method comprises the following steps: S1, preparing a PDRN solution; s2, complementing the double strands of the PDRN into blunt ends; s3, constructing a recombinant plasmid; s4, carrying out mixed culture on a product obtained in the step S3 and escherichia coli DH5alpha; s5, screening the recombinants; s6, carrying out PCR (Polymerase Chain Reaction) amplification, and recording a strain from a fragment with the length of more than 200bp; s7, preparing a seed solution by adopting the strain prepared in the step S6; s8, strain fermentation; and S9, centrifugally collecting thalli. The method comprises the following steps: constructing a recombinant plasmid containing a PDRN fragment, introducing the recombinant plasmid into Escherichia coli, subsequently culturing the recombinant Escherichia coli to copy DNA in the Escherichia coli, extracting a purified exogenous gene fragment to obtain a strain containing a single fragment PDRN sequence, and getting rid of dependence on raw materials during subsequent extraction.

The invention discloses bacillus velezensis as well as fermentation product filtrate and a biological product thereof, and belongs to the technical field of microorganisms, a bacillus velezensis BTN-HB-F4 strain is separated and purified from prinsepia utilis royle in Harba snow mountain in Yunnan province, the fermentation product filtrate of the bacillus velezensis has excellent HAS inhibition capacity and remarkable DPPH and ABTS free radical scavenging capacity, and the biological product of the bacillus velezensis has the advantages that the biological product can be used for preparing biological products of the prinsepia utilis royle; a good anti-oxidation effect can be realized; secondly, under various concentrations, fermentation product filtrate can remarkably reduce the proportion of SA-beta-Gal positive cells, gene expression of matrix metalloproteinase 3 can be remarkably inhibited, and a good anti-aging effect is achieved; in addition, fermentation product filtrate can remarkably inhibit generation of zebra fish melanin and has a good whitening effect; the secretion amount of cell inflammatory factors IL-6 and TNF-alpha can be remarkably inhibited, and a good relieving effect is achieved; finally, the fermentation product filtrate can effectively inhibit the growth of pathogenic bacteria staphylococcus aureus, and has the potential of regulating the skin micro-ecology.

The invention relates to the technical field of microorganisms, and discloses burkholderia gladioli as well as a bacterial agent and application of the burkholderia gladioli. The Burkholderia gladioli (Burkholderia gladioli) CCTCC (China Center For Type Culture Collection) NO: M20242288 provided by the invention has good capacities of fixing nitrogen, dissolving phosphorus, dissolving zinc, producing siderophores and oxidizing sulfur, and can be used for regulating the nutrient structure of soil and converting substances which are difficult to utilize by plants in the soil, so that the growth of the plants is promoted, and the stress resistance of the plants is improved; the strain also can be used for antagonizing Fusarium solani, Fusarium equiseti, Fusarium verticillium, Fusarium verticillium and Rosellinia necatrix, can be used for antagonizing Fusarium solani, Fusarium equiseti, Fusarium verticillium and Rosellinia necatrix, and can also be used for antagonizing Fusarium solani, Fusarium equiseti, Fusarium verticillium, Rosellinia necatrix, Fusarium moniliforme and Rosellinia necatrix, Fusarium moniliforme and Rosellinia necatrix. Besides, the burkholderia gladioli provided by the invention is a strain separated from rape farmland soil, and compared with a strain screened by extreme environment and laboratory mutagenesis, the burkholderia gladioli has better adaptability when being applied to a planting environment, and can be stably colonized in plant rhizosphere and soil, so that the burkholderia gladioli stably plays a growth promoting role for a long time, is safe and environment-friendly, and has a wide application prospect. Wide application prospects are realized.

The invention relates to the technical field of microorganisms, and provides a low-temperature-resistant heterotrophic nitrification-aerobic denitrification pseudomonas and application thereof, the strain is pseudomonas (Pseudomonas sp.) SW1, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 20221797. The low-temperature-resistant heterotrophic nitrification-aerobic denitrification bacterium Pseudomonas sp. SW1 has the characteristic of low temperature resistance, can well grow at 4-10 DEG C, and has relatively strong heterotrophic nitrification-aerobic denitrification nitrogen removal capability, and the enrichment culture of the strain is carried out in a relatively low nitrogen source, and the condition is extremely consistent with the application scene of the strain in domestic sewage.

The invention discloses lactobacillus kefiranofaciens IMAU12319 and application thereof, belongs to the technical field of microorganisms, and particularly relates to lactobacillus kefiranofaciens IMAU12319 which is preserved in China General Microbiological Culture Collection Center (CGMCC) on July 8, 2024, the preservation address is No. 3, No.1 yard, West Beichen Road, Chaoyang District, Beijing, and the number is CGMCC No.31224. The lactobacillus kefiranofaciens IMAU12319 has the advantages that the lactobacillus kefiranofaciens IMAU12319 can be used for preparing the lactobacillus kefiranofaciens IMAU12319, and the lactobacillus kefiranofaciens IMAU12319 can be used for preparing the lactobacillus kefiranofaciens IMAU12319; the invention also relates to an application of the lactobacillus kefiranofaciens IMAU12319 in preparation of a probiotic agent capable of resisting gastrointestinal digestion. The strain disclosed by the invention not only shows good gastrointestinal tract endurance capacity, but also shows relatively strong potential in the aspects of intestinal adhesion, oxidation resistance and bacteriostasis, and has a wide application prospect in leavening agent strains.

The invention belongs to the technical field of microorganisms, and particularly relates to paenibacillus mucilaginosus FangSG101 and application thereof. The preservation number of the paenibacillus mucilaginosus FangSG101 is CCTCC NO: M 20232558, and the paenibacillus mucilaginosus FangSG101 can convert insoluble mineral potassium and mineral phosphorus in soil into quick-acting potassium which can be directly absorbed and utilized by plants, has the capability of generating pathogenic bacterium cell wall hydrolase, has a remarkable promotion effect on seed germination and plant growth of tomatoes and cucumbers, and can be applied to actual agricultural production. Through optimization of a culture medium formula and adjustment of a fermentation process, a bacterial powder product with high viable count and high spore rate is obtained.

The invention discloses bacillus cereus and application thereof, and belongs to the technical field of microorganisms. The bacillus cereus SNF7 is separated from a saline-alkali soil sample, has the functions of resisting salt, fixing nitrogen and promoting growth, has an obvious inhibition effect on some common soil-borne diseases in agriculture, such as pathogenic bacteria of peanut fruit rot, tomato root rot and the like, has multiple functions of resisting salt, fixing nitrogen, biologically preventing and controlling and the like, and has a wide application prospect. The method can be widely applied to crop planting in large saline-alkali soil in China.

The invention relates to the technical field of microorganisms, in particular to plant lactobacillus MPs-68 capable of adsorbing microplastics and degrading uric acid and application of the plant lactobacillus MPs-68. The provided plant lactobacillus MPs-68 capable of adsorbing microplastics and degrading uric acid is a marine-derived natural strain, the preservation number is CGMCC No.31976, the plant lactobacillus MPs-68 has a remarkable adsorption effect on microplastics polystyrene and polyethylene, has the function of degrading uric acid, and is high in oxidation resistance, gastric acid resistance, cholate resistance and intestinal colonization capacity, and the plant lactobacillus MPs-68 can be used for adsorbing microplastics and degrading uric acid. The method has important application value in the fields of food or health care products for assisting adsorption of micro-plastics and reduction of uric acid and the like. The invention also provides a microbial agent, a probiotic product, a functional food, a fermentation product, a microecological preparation, a microplastic adsorbent and a microplastic adsorbent compounded with the lactobacillus brevis MPA01 (the preservation number is CGMCC No.31977) containing the phytobacterium plantarum MPs-68 capable of adsorbing the microplastics and degrading the uric acid, and the microplastic adsorbent has the effects of adsorbing the microplastics, degrading the uric acid and the like.

The invention provides a bacillus subtilis LU5 and a lignocellulose degrading flora as well as a culture method and application thereof, and belongs to the technical field of microorganisms. According to the invention, a strain is screened from surface soil of composted straws and identified as bacillus subtilis, the strain is combined with bacillus velezensis SS6 and trichoderma longibrachiatum according to a specific proportion, co-culture is carried out in a specific culture medium, a flora capable of degrading lignocellulose is obtained, all microorganisms of the flora are in synergistic symbiosis, and the effect of degrading lignocellulose is achieved. The produced various cellulases have a synergistic effect, so that the aim of degrading lignocellulose is fulfilled. And by adopting the flora provided by the invention, the efficiency and the stability of the lignocellulose degradation process are remarkably improved.

The invention belongs to the technical field of microorganisms, particularly relates to rice endophytic bacillus velezensis AN6 and application thereof, and particularly provides bacillus velezensis AN6 which is classified and named as bacillus velezensis and has the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20231886. The invention provides a novel rice endophytic bacillus velezensis strain AN6, through separation, screening, identification and experimental verification, it is found that the strain shows broad-spectrum resistance to various phytopathogens including rice bacterial leaf blight, rice blast and the like, the strain has the capacity of inhibiting various phytopathogens and has the capacity of adsorbing heavy metal, and the strain has the advantages of being capable of inhibiting various phytopathogens and capable of being used as a novel rice endophytic bacillus velezensis strain AN6. The method is of great significance to biological control of rice diseases and development of green agriculture.

The invention relates to lactobacillus rhamnosus CBBM005 and application thereof in products for balancing intestinal flora, improving immunity and improving oral cavity, and belongs to the technical field of microorganisms. The lactobacillus rhamnosus CBBM005 is separated from human intestinal secretion, the lactobacillus rhamnosus CBBM005 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation time is March 20, 2025, and the preservation number is CGMCC NO.33904. The lactobacillus rhamnosus CBBM005 has the characteristics of balancing intestinal flora, improving immunity and improving oral cavity.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus mobilis DG-7 and application thereof in inhibiting bolting of angelica sinensis and improving disease resistance of angelica sinensis. The invention provides a strain of bacillus mobilis DG-7, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.31089. The bacillus mobilis DG-7 and the microbial inoculum thereof provided by the invention can play roles in inhibiting early bolting of angelica sinensis and improving disease resistance by increasing the content of plant endogenous melatonin and salicylic acid, reducing the content of abscisic acid, auxin and gibberellin, reducing the content of total phenolic acid and reducing the expression of photoperiod-related genes. In addition, the bacillus mobilis DG-7 does not solubilize phosphorus, so that the promotion effect of high-concentration phosphorus on early bolting is effectively avoided; the laccase activity of the bacillus mobilis DG-7 is high, polyphenol substances can be effectively removed, and the bolting rate can be reduced.

The invention relates to the technical field of microorganisms, and in particular relates to application of a strain of lactobacillus mucus TG018. The invention further relates to a preparation method of the lactobacillus mucus TG018. The preservation number of the lactobacillus mucus TG018 is CCTCC (China Center for Type Culture Collection) No: M 2023514; the lactobacillus mucus TG018 can be used for producing nicotinic acid, serving as an antioxidant and / or preparing the antioxidant, or can be applied to medicines for reducing uric acid.

The invention discloses aureobasidium pullulans, application thereof and a method for producing medium-molecular-weight pullulan, and belongs to the technical field of microorganisms. The aureobasidium pullulans disclosed by the invention is aureobasidium pullulans SYCY-018, the strain is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on December 26, 2022, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.40467. According to the aureobasidium pullulans SYCY-018, the aureobasidium pullulans SYCY-018 and the aureobasidium pullulans The aureobasidium pullulans SYCY-018 is inoculated into a fermentation medium for fermentation to produce the pullulan, the yield of the medium-molecular-weight pullulan reaches up to 150 g / L or above, meanwhile, good hereditary stability and high temperature resistance are achieved, the yield of the pullulan can still be higher than 130 g / L even at the high temperature of 40 DEG C, and the aureobasidium pullulans SYCY-018 is suitable for industrial production.

The invention discloses an endophytic fungus LW111 and application thereof, and belongs to the technical field of microorganisms. The endophytic fungus LW111 is classified and named as Magnaporthaceae sp.LW111, and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 20241750. The endophytic fungus LW111 is co-cultured with rice, so that the endophytic fungus LW111 is colonized in the root tissue of the rice, the tolerance of rice plants to cadmium, especially in the seedling stage, can be enhanced, and the growth of the plants is promoted. Therefore, the endophytic fungus LW111 has popularization and application values in the aspects of guaranteeing plant health and improving rice seedling stage growth.

The invention relates to bacillus velezensis and application thereof in preparation of a water-soluble microbial agent, and belongs to the technical field of microorganisms. The bacillus velezensis has the effects of producing exopolysaccharides, promoting the formation of soil aggregates, degrading abamectin, hydrolyzing protein, producing indoleacetic acid, promoting the growth of tomato plants and promoting the enzyme activity of tomato rhizosphere soil enzyme. The fertilizer not only has the effects of improving the soil aggregate structure and promoting the growth of tomato plants, but also can accelerate the temperature rise of the abamectin fermentation waste residue compost, prolong the high-temperature period and shorten the compost period, and is beneficial to promoting the harmless treatment and resource utilization of the abamectin fermentation waste residue.

The invention relates to bacillus velezensis and application thereof in preparation of a multifunctional straw decomposition agent, and belongs to the technical field of microorganisms. The bacillus velezensis has the functions of producing cellulase, producing xylanase, degrading abamectin, decomposing inorganic phosphorus and degrading straws; when straw is returned to the field, the multifunctional straw decomposition agent provided by the invention not only can accelerate the decomposition speed of straw, decompose abamectin residues in soil and improve the soil ecology, but also can promote the growth of pepper plants and enhance the stress resistance of the pepper plants, and has a good comprehensive application effect.

The invention relates to the technical field of microorganisms, in particular to application of Cladosporium LC3 in crop growth promotion and bacterial wilt resistance, the strain LC3 belongs to Cladosporium and Cladosporium immonda species, the strain is separated from Guangxi sugarcane rhizosphere soil, experimental evaluation screening finds that the strain has a good growth promotion function on tomatoes and fresh ginger, and the strain can be applied to crop growth promotion and bacterial wilt resistance. The yield of ginger is remarkably increased, and the growth vigor of tomato plants is effectively improved; meanwhile, the strain has a good prevention and treatment effect on tomato and ginger bacterial wilt and has good plant bacterial wilt resistance, and screening verification of the strain provides a new fungicide and a raw material for later-period growth of an anti-microbial agent or a biological organic fertilizer.

The invention discloses a compound microbial agent for enhancing production of beta-phenethyl alcohol in a white spirit brewing process as well as a preparation method and application of the compound microbial agent, and belongs to the technical field of microorganisms. The compound microbial agent is prepared from aspergillus niger, pichia membranaefaciens, Kazakstan monospora, saccharomyces cerevisiae and sporospora delbrueckii; in the compound microbial agent, the final concentration OD600 of the aspergillus niger, the final concentration OD600 of the pichia membranaefaciens, the final concentration OD600 of the Kazakstein monospora, the final concentration OD600 of the saccharomyces cerevisiae and the final concentration OD600 of the sporospora delbrueckii are 0.8-1.5, 0.8-1.5, 0.5-1, 0.8-1.5 and 0.5-1 respectively. After the compound microbial agent provided by the invention is applied to a solid-state fermentation system of Luzhou-flavor liquor, the yield of the aroma substance beta-phenethyl alcohol can be obviously increased by adjusting the eukaryotic microbial community structure of the fermentation system; the method can effectively solve the problem that the stability of the method for producing beta-phenethyl alcohol in the process of intensifying Baijiu fermentation by a single strain is poor.

The invention discloses bifidobacterium breve BBr69 with an immunoregulation function as well as application, a product and a method thereof, and belongs to the technical field of microorganisms. The preservation number of the bifidobacterium breve BBr69 with the immunoregulation function is CGMCC (China General Microbiological Culture Collection Center) NO. 32425. Based on the strain, the invention also provides application and related products of the strain. The BBr69 strain has broad-spectrum antibacterial performance, has the function of regulating intestinal flora and also has the function of enhancing immunity, and immunity is enhanced by proliferating RAW264.7 cells, increasing the content of cell factors, inhibiting weight loss, increasing the index of immune organs and reducing ileum tissue damage.

The invention discloses a cladosporium sp. Fungus YS37 and application thereof in promoting rice growth, and belongs to the technical field of microorganisms. The cladosporioides sp. Fungus YS37 is a new species separated from the root system of wild rice, is named as Parasarocladium oryzae YS37, is preserved in the China Center for Type Culture Collection (CCTCC), and has a preservation number of CCTCC NO: M 20242286, and the cladosporioides sp. Fungus YS37 is a new species which is separated from the root system of wild rice and is named as Parasarocladium oryzae YS37. The Parasarocladium oryzae YS37 strain can be used for preparing a biological bacterial fertilizer, and when the Parasarocladium oryzae YS37 strain or a fermentation product of the Parasarocladium oryzae YS37 strain is applied to a rice root system, the growth of rice can be effectively promoted, and the plant biomass is increased. The invention provides a new way for application of microorganisms to development of crop production.

The invention belongs to the technical field of microorganisms, and relates to a lactobacillus paracasei JNL0025 strain and application thereof. Compared with a commercial strain lactobacillus casei substitute strain, the lactobacillus paracasei JNL0025 provided by the invention has the advantages that the guanine nucleoside conversion rate is improved, the inhibition rate of supernate on XOD is equivalent, and the inhibition on PNP activity can be realized; according to the lactobacillus paracasei JNL0025 disclosed by the invention, the lactobacillus paracasei JNL0025 has the effects of reducing the tendency of pro-inflammatory M1 type macrophages, increasing the proportion of anti-inflammatory M2 type macrophages and inhibiting secretion of pro-inflammatory cytokines TNF-alpha and IL-6, and the lactobacillus paracasei JNL0025 has a better inflammation inhibition effect than the lactobacillus rhamnosus LGG; animal experiment results show that the lactobacillus paracasei JNL0025 provided by the invention can significantly reduce the blood uric acid level of a hyperuricemia model mouse, alleviate the kidney inflammation level and alleviate DSS-induced colitis, and shows a huge application value.

The invention belongs to the technical field of microorganisms, and discloses a low-temperature-resistant aerobic denitrifying bacterium and application thereof. The low-temperature-resistant aerobic denitrifying bacterium is Enterobacter ludwigii CW-01, and the preservation number of the low-temperature-resistant aerobic denitrifying bacterium is CGMCC (China General Microbiological Culture Collection Center) No.27126. The low-temperature-resistant aerobic denitrifying bacterium can be used for preparing the The enterobacter lewis CW-01 strain disclosed by the invention can efficiently realize aerobic denitrification degradation of ammonia nitrogen, nitrate and nitrite within the temperature range of 4-35 DEG C, and is especially suitable for wastewater treatment in which the ammonia nitrogen, nitrate and nitrite are difficult to remove due to low temperature; the strain also has good salt resistance, and provides a good microbial material for biological nitrogen removal treatment of low-temperature, high-salt and high-ammonia nitrogen polluted water. The nitrogen is efficiently removed, meanwhile, the use amount of a carbon source is remarkably reduced, the enterprise cost is greatly reduced, and the method has a good application prospect in the field of sewage treatment.

The invention relates to a salt-tolerant growth-promoting pseudomonas putida PLW1 and application thereof, and belongs to the technical field of microorganisms, the pseudomonas putida PLW1 is preserved in China Center for Type Culture Collection on June 24, 2024, and the preservation number is CCTCC NO: M 20241352; the strain has the functions of degrading organic phosphorus, degrading inorganic phosphorus, producing lipase, producing siderophores and producing indoleacetic acid, and also has good salt-resistant and alkali-resistant characteristics, and the microbial inoculum prepared from PLW1 can promote germination of corn seeds and growth of corn seedlings under salt stress, and has a very wide application prospect.

The invention discloses bifidobacterium animalis subsp. Lactis BLa79 as well as application, a product and a method thereof, and belongs to the technical field of microorganisms. The preservation number of the bifidobacterium animalis subsp. Lactis BLa79 is CGMCC (China General Microbiological Culture Collection Center) NO. 32426, and the preservation number of the bifidobacterium animalis subsp. Lactis BLa79 is CGMCC NO. 32426. Based on the strain, the invention also provides application and related products of the strain. The BLa79 strain has broad-spectrum antibacterial performance, has the function of regulating intestinal flora, can effectively relieve ulcerative colitis, and can relieve colitis by increasing body weight, reducing DAI score, reducing spleen index, inhibiting colonic atrophy, reducing proinflammatory factor level and promoting SIgA secretion.

The invention relates to the technical field of microorganisms, in particular to application of pediococcus acidilactici. The pediococcus acidilactici TG005 is preserved in the China Center for Type Culture Collection on June 24, 2024, the preservation number is CCTCC NO: M 20241349, and the preservation address is Wuhan University, Wuhan, China. The pediococcus acidilactici TG005 provided by the invention is a multifunctional probiotic strain, is obtained by screening from domestic healthy human excrement samples, and has good application safety and functional adaptability; the strain can reduce the cholesterol level and prevent cardiovascular diseases; uric acid precursor substances are degraded, uric acid generation is reduced, and hyperuricemia and related diseases are prevented; pathogenic bacteria with fusobacterium nucleatum and bacteroides fragilis can be inhibited, and colorectal cancer is prevented; when being used as a probiotic additive for preparing medicines for degrading cholesterol, preventing cardiovascular diseases caused by high cholesterol, preventing hyperuricemia, colorectal cancer and the like, the lactobacillus plantarum has a wide application prospect.

The invention relates to the technical field of microorganisms, in particular to a viable bacterium preparation of a lactobacillus paracasei strain as well as a preparation method and application of the viable bacterium preparation. The viable bacterium preparation comprises a lactobacillus paracasei strain and auxiliary materials, and the preservation number of the lactobacillus paracasei strain is CGMCC (China General Microbiological Culture Collection Center) No.27974. The viable bacterium preparation can inhibit the growth of streptococcus mutans, reduce the formation of a dental plaque biofilm caused by the streptococcus mutans, reduce the content of a mature dental plaque biofilm, inhibit the level of water-insoluble exopolysaccharides generated by the streptococcus mutans, reduce the expression quantity of cariogenic genes gtfB, gtfC, gtfD, ftf and LuxS of the streptococcus mutans, improve the cariogenic activity of the streptococcus mutans and improve the cariogenic activity of the streptococcus mutans. The adhesion rate of streptococcus mutans on a hydroxyapatite tooth model is reduced, and a beneficial in-vitro probiotic function is achieved.

The invention discloses construction and application of an L-malic acid producing issatchenkia orientalis strain, and belongs to the technical field of microorganisms and the technical field of microbial fermentation and metabolic engineering. Through a metabolic engineering means, a cytoplasm metabolic pathway of an issatchenkia orientalis strain is transformed, and a pyruvate decarboxylase coding gene, glycerol dehydrogenase and lactic dehydrogenase are knocked out; a pyruvate carboxylase gene derived from aspergillus flavus, a malate dehydrogenase gene derived from corynebacterium glutamicum and phosphoenolpyruvate carboxylase derived from escherichia coli are screened and integrated, so that a cytoplasm rTCA cycle synthesis pathway is enhanced; inserting a dicarboxylic acid external transporter gene from schizosaccharomyces cerevisiae at a neutral site GD21, and knocking out endogenous dicarboxylic acid internal transporter; the L-malic acid yield of the modified yeast strain in the fermentation process is remarkably increased and reaches 140.16 g / L. The engineering strain provided by the invention has good industrial application potential in efficient L-malic acid production.