57results about How to "Disease prevention and control" patented technology

The invention discloses Bacillus velezensis and its application in plants and provides a strain of Bacillus velezensis with broad-spectrum disease-preventing and life-promoting activity in a plant anda microbial agent containing the strain as an active ingredient. The Bacillus velezensis is Bacillus velezensis NSZ-YBGJ0001 and has a preservation number of CGMCC No. 14384. The Bacillus velezensisNSZ-YBGJ0001 with the preservation number of CGMCC No. 14384 can effectively prevent and control plant diseases, does not harm to the plants and can promote plant growth to a certain extent. The Bacillus velezensis microbial agent can replace or reduce chemical pesticides, protect the ecological environment to a large extent and keep the quality and safety of agricultural products.

The invention discloses a bacillus amyloliquefaciens strain as well as a preparation method and application of a strain powder preparation of the bacillus amyloliquefaciens strain, and belongs to the technical field of microorganisms. The bacillus amyloliquefaciens JSSW-LA, which is obtained through screening, is preserved in China Center for Type Culture Collection with preservation number of CCTCC NO: M2015602. The strain powder preparation of the bacillus amyloliquefaciens, which is prepared through freeze-dried culture activation, fermentation culture and strain powder preparation, is applicable to an additive of fishery breeding feed or a water quality improver of aquaculture; the bacillus amyloliquefaciens JSSW-LA provided by the invention is capable of effectively inhibiting the growth of A.sobria, A.hydrophila, A.caviae, aeromonas veronii and edwardsiella tarda, and an inhibitory effect on the A.sobria, A.hydrophila and A.caviae is more excellent than that on other pathogenic bacteria. Therefore, the JSSW-LA strain powder, applied to aquaculture, is capable of effectively preventing and controlling diseases in aquaculture animals caused by pathogenic bacteria, in particular bacterial bleeding disease, so as to protect the healthy growth of aquatic animals.

The invention relates to a biological control agent, a bioorganic fertilizer, and applications thereof. The biological control agent comprises Bacillus velezensis, Bacillus subtilis, Paenibacillus polymyxa, and Bacillus amyloliquefaciens. The provided biological control agent and bioorganic fertilizer can continuously inhibit fusarium oxysporum for a long term, and has a long-lasting control effect on plant blight, in particular, banana blight.

The invention belongs to a fruit tree yellowing disease preventing and treating comprehensive preparation in fruit tree fertilizers. The preparation comprises (1) a foliage spray micro element fertilizer comprising the following components in percentage by weight: 18-22% of sequestration iron, 13-17% of magnesium, 13-17% of boron, 18-22% of zinc, 9-11% of silicon, 9-11% of selenium, 9-11% of kalium, 0.02% of endogenous hormone and 0.02% of gibberellin, total being 100%, and adding 500-700 times of water for foliage spray; (2) a root-irrigation conditioner comprising the following components in percentage by weight: 11-14% of sequestration iron, 45-55% of citric acid, 2-3% of soil conditioner, 30-40% of nitrogen, phosphorus and potash fertilizers which are same in amount, and trace of protease, total being 100%; and adding 40-60 times of water for the root-irrigation; and (3) a stem-coating nutritional agent comprising the following components in percentage by weight: 18-22% of sequestration iron, 0.1-0.3% of humic acid, 0.1-0.3% of fulvic acid, trace of aspartic acid and threonine and the balance of soft water penetrating agent, total being 100%; and adding 10-20 times of water for stem-coating. After kiwi fruit flowers, before a fruit expanding period (the first-ten and middle ten days of June), the foliage spray micro element fertilizer (the sequestration iron and multiple micro element nutrient solution) can be sprayed on leaves, meanwhile the conditioner (multiple nutrients and enzyme compounds and endogenous root-generating healing agents) is performed. A leaf fast green comprehensive preventing and treating preparation is coated on stems for the serious fruits. Triple effect is united as one, and the purpose of curing the yellowing disease is achieved.

The invention discloses a bacillus velezensis DH82 which is deposited on December 4, 2018 in China General Microbiological Culture Collection Center with the preservation number of CGMCC No. 16865. The method further discloses a preparation method of an antibacterial protein thereof by using the bacillus velezensis DH82 and the application of the antibacterial protein in an aquaculture feed additive. The antibacterial protein can effectively prevent and control diseases of aquaculture animals caused by aquatic pathogenic bacteria, thereby protecting the healthy growth of aquatic animals. The protein can be used as a feed additive for fish farming to significantly improve the immunity of the aquatic animals and has a good application prospect.

The invention discloses a growth-promoting bacteria combination for enhancing salt tolerance of crops in moderate-severe saline and alkaline lands. The growth-promoting bacteria combination comprises a bacillus amyloliquefaciens strain ZCM18 and a bacillus tequilensis strain. Preservation numbers of the two strains are CGMCC No.1295 and CGMCC No.12960 respectively. The two strains are capable of tolerate the salinity of 5%, have high capabilities of phosphate solving, nitrogen fixing and producing IAA (indoleacetic acid), ACCD (acetyl-CoA carboxylase subunit D), siderophore and cytokinin, and can antagonize root pathogenic bacteria including fusarium, rhizoctonia, pythium and the like. The two stains after being combined are suitable for the moderate-severe saline and alkaline lands (with salt content ranging from 0.4% to 1.0%), and in the production field of biological fertilizer for salt tolerating and growth benefiting for the crops, the growth-promoting bacteria combination is promising in prospects of crop's salt tolerance enhancement, crop root growth benefiting, seedling existing rate increasing, fertilizer using amount decreasing and crop yield increasing.

The invention discloses bacillus coagulans having an aquatic pathogen antagonistic characteristic and application of the bacillus coagulans, and belongs to the technical field of microorganisms. The strain is classified and named as bacillus coagulans JSSW-LA-07-1 and is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC No. 10182. The invention also discloses a preparation method of a strain powder preparation, wherein the method comprises the following steps: activating and fermentation-cultivating the bacillus coagulans JSSW-LA-07-1 which serves as an initial strain, so that a fermentation broth is obtained, centrifuging the fermentation broth at a high speed and collecting a wet strain; mixing the wet strain with dry starch in the mass ratio of 1 to (2-10); drying at 30-50 DEG C for 20-24h; grinding by virtue of a grinder; and filtering by virtue of a 0.9mm sieve, so that the strain powder preparation is obtained; in the strain powder preparation, bacterium concentration is no less than 5.0 to the power of 8 (CFU)/g; the strain powder preparation, when used as an additive of fish culture feed, is added by 1.0-5.0mg/kg to feed basal diet; and the strain powder preparation, when used as a water quality improver for aquaculture, is sprayed to a full tank by the dosage of 50-200g/mu.m (mu is a unit of area, equal to 0.0667 hectares).

The invention provides a pesticide composition and application thereof, belonging to the technical field of mixed pesticides. The pesticide composition comprises an active component A and an active component B, wherein the active component A is methiadinil, the active component B is a plant growth regulator, and a weight ratio of the active component A to the active component B is in a range of 2: 1 to 2500: 1, preferably 10: 1 to 1000: 1. The plant growth regulator B may be uniconazole, brassinolide, indolebutyric acid, compound sodium, nitrophenolate, butralin, or the like. Compounding of the active component A and the active component B exerts obvious synergistic effect, and the respective application amounts of the active component A and the active component are reduced. Any dosage form of the composition can be prepared by adding auxiliary components acceptable in other pesticides. The pesticide composition provided by the invention is mainly applicable to crops like paddy rice, corn and rape, and can induce the crops to generate systematic disease resistance, effectively control pathogenic fungi of plants, prevent and treat a plurality of diseases like tomato virus diseases, wheat powdery mildew, sclerotinia rot of colza, cabbage clubroot, rice blast, wheat sharp eyespot and grape downy mildew, and regulate plant growth to realize yield increase of the crops.

The invention provides a Chinese herbal medicine additive used in egg chicken feed. The Chinese herbal medicine additive is prepared from the following components in parts by weight: 25-35 parts of Callicarpa macrophylla, 15-25 parts of mulberry leaf, 15-25 parts of folium cortex eucommiae, 5-10 parts of Angelica sinensis, 5-15 parts of semen cuscutae and 5-15 parts of longspur epimedium. The Chinese herbal medicine additive is applied to the egg chicken breeding and can substitute antibiotics to prevent and control diseases in an egg chicken breeding process, the morbidity of the chicken flocks is reduced, the death and culling rate is reduced, green and safe eggs without medicine residue can be produced, the egg quality is improved, the peak laying period can be prolonged to be more than 60 days, and the laying quantity can be increased by more than 12%.

The invention provides a bioorganic fertilizer with a Bok choy anthrax control function. The raw materials of the bioorganic fertilizer comprise Paenibacillus polymyxa, Bacillus subtilis, a compost produced from animal manure and plant residues, and a poly(gamma-glutamic acid) solution. The invention also provides a preparation method of the bioorganic fertilizer. The preparation method comprises the following steps: inoculating he compost produced from the animal manure and the plant residues with a mixed bacterial liquid prepared after culturing and activating two antagonistic bacteria comprising Paenibacillus polymyxa and Bacillus subtilis, carrying out fermentation to obtain a decomposed product, adding the a poly(gamma-glutamic acid) solution, and drying the obtained mixture in a shady place to prepare the bioorganic fertilizer with a Bok choy anthrax control function. The bioorganic fertilizer prepared in the invention has a substantial control effect on Bok choy anthraxes; and the preparation method of the product has the advantages of simplicity, wide sources of the raw materials, and low production cost, and is suitable for large-scale production of the bioorganic fertilizer with the Bok choy anthrax control function.

The invention relates to a preparation method of a liquid fertilizer containing microorganisms. The preparation method comprises: adding plant residues, an acid-alkali adjusting agent, distilled water, a culture medium and microorganisms into a fermentation tank, fermenting at a certain temperature, carrying out pH value adjustment and cooling on the product after fermenting for a certain time, storing for 3-5 days in the fermentation tank, adding slaked lime, adjusting the pH value, continuously adding the culture medium and the beneficial microorganism to the fermentation tank, fermenting at a certain temperature, carrying out pH value adjustment and cooling on the product after the fermenting, storing for 3-5 days in the fermentation tank, repeating the steps one time, carrying out press filtration on the product to obtain filtrate, and adding the filtrate to a liquid fertilizer to obtain the liquid fertilizer containing the microorganisms. According to the present invention, the natural ecological environment of the soil is simulated, and the three fermentations are performed, such that the fermented microbial agent can quickly adapt to the environment after being put into the soil so as to ensure the survival rate.

The invention provides bacillus for biological prevention and control of root rot and bacterial wilt. The strain is bacillus subtilis FJAT-19708, has a scientific name of Bacillus subtilis FJAT-19708and is preserved in China General Microbiological Culture Collection Center (CGMCC), with the preservation number of CGMCC No. 14431. The bacillus FJAT-19708 screened by the invention has a very strong inhibition effect on main pathogenic bacteria including fusarium oxysporum and fusarium solani of the root rot, and also has a very strong antagonistic effect on ralstonia solanacearum; the bacilluscan be used for preventing and controlling the root rot and also can be used for preventing and controlling the bacterial wilt.

The invention specifically relates to an Oocystis concentrate with high activity and high stress resistance, and a preparation method and application thereof, belonging to the technical fields of aquaculture water quality regulation and water environment treatment. The preparation method comprises the following steps: isolation and cultivation of purebred Oocystis; seed selection of Oocystis withhigh activity and high stress resistance; enlarged cultivation; industrial cultivation; collection; concentration; and the like, wherein continuous aerated cultivation is carried out under the conditions of a temperature of 23 to 28 DEG C and an illumination of 1800 to 2000 Lx. Compared with the prior art, the concentration of the Oocystis concentrate of the invention can reach 130 to 150 million/ml, can be stored at normal temperature for 95-105 days, has a survival rate of 95% or more, and is convenient for storage, transportation and usage; and the Oocystis concentrate has high activity, high stress resistance and stable algal phase, can keep the water quality of a culture pond in benign equilibrium for a long time, is applicable to preparation of algal phase stabilizers for seawater orbrackish-water culture ponds of marine food products, and shows good industrial application prospects.

The invention provides antibiotic-free, environment-friendly and high-energy layer feed. The technical scheme is that the antibiotic-free, environment-friendly and high-energy layer feed comprises components in percentage by weight as follows: 10wt%-30wt% of grease, 10wt%-30wt% of 43% soybean meal, 10wt%-30wt% of corn DDGS, 5wt%-10wt% of chrysanthemum powder, 10wt%-30wt% of corn spraying fibers, 4wt%-10wt% of calcium hydrophosphate, 10wt%-20wt% of mountain flour, 1wt%-3wt% of sodium chloride, 1wt%-3wt% of sodium bicarbonate, 0.1wt%-0.3wt% of a multivitamin premix, 0.1wt%-0.3wt% of a multi-mineral premix, 0.1wt%-0.5wt% of phytase 10000, 1wt%-3wt% of self-made compound enzyme, 0.1wt%-0.5wt% of microecological bacteria, 0.1wt%-0.2wt% of 70% choline chloride, 0.5wt%-1.0wt% of 70% feed-grade lysine, 0.1wt%-0.5wt% of methionine, 0.1wt%-0.5wt% of threonine, 0.05wt%-0.15wt% of tryptophan, 0.05wt%-0.15wt% of valine, 0.1wt%-0.4wt% of a solid antioxidant and 0.1wt%-0.3wt% of a mildew preventive. With the adoption of compound feed prepared from the antibiotic-free, environment-friendly and high-energy layer feed, feed energy can be supplemented, balance between crude protein and energy is kept, and digestive absorption of layers is promoted; the compound enzyme is a self-compounded mixture of cellulase, xylanase, pectinase, glucanase, mannase, xylanase and the like, and can effectively degrade fibrous matter and high molecular weight protein and increase digestive rate of nutritional ingredients.

The invention relates to the technical field of biological control, and particularly discloses bacillus velezensis, a microbial agent thereof, a preparation method of the microbial agent and application of the bacillus velezensis. The collection number of the bacillus velezensis is CGMCC NO.19860; the collection unit of the bacillus velezensis is China General Microbiological Culture Collection Center; the collection name of the bacillus velezensis is bacillus velezensis WSW2,; and the collection time of the bacillus velezensis is May 21, 2020. The bacillus velezensis has the characteristics that the prevention and control effect is good and tomatoes cannot be polluted.

The invention belongs to the field of biocides, and discloses a vegetable tannin antibacterial agent with a body structure, and a preparation method and application thereof. A nucleophilic reagent isused for degrading vegetable tannin, and then a phenolic aldehyde condensation reaction is carried out, so that the molecular weight of a vegetable tannin antibacterial agent product is increased, andthe number of C6C6 bonds is increased; therefore, the obtained product has the structural characteristic of 'space body type'; meanwhile, in the phenolic aldehyde condensation reaction process, the reaction process can be carried out uniformly by virtue of the non-thermal effect of a microwave field, meanwhile, the reaction speed is increased, and finally, the vegetable tannin antibacterial agentwith a highly branched molecular structure, large molecular weight and a stable physical and chemical state is obtained. Meanwhile, through a mode of firstly degrading and then condensing, the defectthat the molecular weight is difficult to control or precipitates are easy to form due to overlarge molecular weight caused by direct condensation without degradation is avoided. The vegetable tanninantibacterial agent disclosed by the invention is a broad-spectrum antibacterial agent, is suitable for preparing biocides and has a wide application prospect.

The invention discloses a method for controlling postharvest storage diseases before picking. The method comprises the following steps: after grape blossom, respectively spraying calcium propionate solution with a final concentration of 0.5-1.5% (w/v) in a young fruit period, in a middle fruit period, in a mature period and before picking; and after picking, grapes are put into a refrigerator or acold storage, wherein the preservation period is prolonged by 30 days or above compared with the preservation period of grape fruits which are not treated. The method disclosed by the invention has an obvious inhibition effect on common storage diseases of grapes; meanwhile, according to laboratory bacteriostatic experiments and field practical applications, the fruit cracking rate in the early stage of the grapes can be obviously reduced, the incidence rate and the falling rate of grapes in the field and in a post-harvest storage process can be inhibited, and the fruit quality can be well maintained. The method disclosed by the invention has the advantages of simple flow, no toxicity, no pollution and low cost and has a huge practical application value.

The invention discloses wild papaya foliar bacterial fertilizer as well as a preparation method and application thereof. The wild papaya foliar bacterial fertilizer is prepared from 20-40 parts of bacillus cereus, 6-10 parts of chitosan, 6-10 parts of amino acids, 10-20 parts of micro-element fertilizer and 20-40 parts of water. The wild papaya foliar bacterial fertilizer is applied to the aspectsof increasing the yield of wild papaya, improving the quality of the wild papaya, improving the storage performance of the wild papaya, realizing the induced enhancement of the disease resistance ofthe wild papaya, promoting the growth of the wild papaya plants, and the like. The wild papaya foliar bacterial fertilizer has the advantages of being remarkable in effects, green and free from pollution and high in fertilizer utilization rate, preventing and controlling diseases, improving yield and quality, being low in cost and easy to popularize, and the like.

The invention relates to a Chinese herbal medicine compound feed additive suitable for fattening pigs and a preparation method of the Chinese herbal medicine compound feed additive. The compound feed additive comprises 15-20 parts of a Chinese herbal medicine composition, 1-3 parts of amino acid, 1-3 parts of compound probiotics, 0.5-2 parts of an enzyme preparation, 1-3 parts of trace elements and 18-29 parts of wheat bran. The Chinese herbal medicine composition comprises 10-15 parts of astragalus membranaceus, 0.5-2 parts of ginseng, 10-15 parts of eucommia ulmoides, 1-5 parts of medlar, 10-15 parts of honeysuckle flowers, 2-5 parts of cortex fraxini, 0.5-2 parts of codonopsis pilosula, 4-8 parts of fermented soybean, 2-5 parts of burdock, 10-15 parts of epimedium, 5-15 parts of acanthopanax roots, 10-13 parts of purslane, 0.5-1 part of mint, 10-20 parts of pericarpium citri reticulatae, 1-5 parts of scutellaria baicalensis, 10-15 parts of schizonepeta and 5-10 parts of liquorice roots. The Chinese herbal medicine compound feed additive solves the problems of high morbidity, slow growth speed and high feed-gain ratio of the fattening pigs, and solves the problems of abuse of antibacterial drugs and drug residues.