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Preparation method of liquid fertilizer containing microorganisms

A technology of microorganisms and microbial agents, applied in the direction of microorganism-based methods, preparation of organic fertilizers, biochemical equipment and methods, etc., can solve the problems of inability to exert the effect of microbial agents, death of "indigenous" microorganisms, and inability to form population effects, etc. problems, to achieve the effect of improving crop quality, strong survival competitiveness, and strong acid resistance

Inactive Publication Date: 2016-11-09
纪新强
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the serious soil compaction and acidification, the external temperature is erratic, and the soil environment has its own "indigenous" microorganisms, the microbial agents cultivated in the greenhouse are directly put into the soil, because they cannot adapt to the harsh environment and the competition of "indigenous" microorganisms in the soil. Basically all of them died, even if a small number survived because they could not form a population effect, they would not be able to exert the effect of microbial agents

Method used

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  • Preparation method of liquid fertilizer containing microorganisms
  • Preparation method of liquid fertilizer containing microorganisms

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Add kelp, soybean meal, wheat bran, slaked lime and distilled water into the fermenter according to the mass ratio of 200:500:300:1:350 and sterilize at 120°C for 20 minutes to obtain product A; Bacillus was inoculated into a liquid medium at 35°C and cultured for 26 hours; the liquid medium was: glucose 12g, peptone 4g, soluble starch 2g, potassium nitrate 0.6g, dipotassium hydrogen phosphate 0.2g, magnesium sulfate 0.8g, sulfuric acid Zinc 0.01g, sodium chloride 1g, agar 15g, distilled water 1000ml, pH6.8; the ratio of Bacillus subtilis and liquid medium is 8:100; the seed liquid is added to product A, wherein the quality of the seed liquid and product A The ratio is 8:100, anaerobic fermentation at 35°C, pH 6.5-7.5 for 8 days to obtain product B; adjust the pH of product B to 5.0-5.5, cool the temperature of the fermenter to 4°C, store for 4 days, and The temperature was restored to 35°C to obtain product C; the seed liquid was added to product C, wherein the mass ra...

Embodiment 2

[0037] Add kelp, Enteromorpha, wheat bran, slaked lime and distilled water into the fermenter according to the mass ratio of 200:500:300:1:350 and sterilize at 130°C for 15 minutes to obtain product A; and prepare fermented seed liquid: Brazil Azospirillum and Bacillus subtilis were inoculated into the liquid medium at 35°C and cultured for 26 hours; the liquid medium was: the liquid medium was: glucose 12g, peptone 4g, soluble starch 2g, potassium nitrate 0.6g, Dipotassium hydrogen phosphate 0.2g, magnesium sulfate 0.8g, zinc sulfate 0.01g, sodium chloride 1g, agar 15g, distilled water 1000ml, pH6.8; the ratio of Azospirillum brasiliensis, Bacillus subtilis and liquid medium is 4:4 : 100; seed liquid is added in product A, wherein the mass ratio of seed liquid and product A is 8:100, at 35 ℃, aerobic fermentation 6 days under the condition of pH6.5~7.5, obtain product B; Adjust product B pH to 5.0-5.5, cooling the temperature of the fermenter to 4°C, storing for 3 days, retur...

Embodiment 3

[0039]Add kelp, enteromorpha, wheat bran, slaked lime and distilled water into the fermenter according to the mass ratio of 200:500:300:2:300 and sterilize at 115°C for 25 minutes to obtain product A; and prepare fermented seed liquid: Subtilis subtilis Bacillus was inoculated into the liquid medium at 30°C and cultured for 26 hours; the liquid medium was: the liquid medium was: glucose 12g, peptone 4g, soluble starch 2g, potassium nitrate 0.6g, dipotassium hydrogen phosphate 0.2 g, magnesium sulfate 0.8g, zinc sulfate 0.01g, sodium chloride 1g, agar 15g, distilled water 1000ml, pH6.8; the ratio of Bacillus subtilis and liquid medium is 8:100; the seed liquid is added to product A, Wherein the mass ratio of seed liquid to product A is 8:100, at 30 DEG C, under the condition of pH6.5~7.5 aerobic fermentation 5 days, obtain product B; Adjust the pH of product B to 5.0~5.5, cool down fermentor temperature to Store at 5°C for 3 days, return the temperature to 30°C to obtain produc...

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Abstract

The invention relates to a preparation method of a liquid fertilizer containing microorganisms. The preparation method comprises: adding plant residues, an acid-alkali adjusting agent, distilled water, a culture medium and microorganisms into a fermentation tank, fermenting at a certain temperature, carrying out pH value adjustment and cooling on the product after fermenting for a certain time, storing for 3-5 days in the fermentation tank, adding slaked lime, adjusting the pH value, continuously adding the culture medium and the beneficial microorganism to the fermentation tank, fermenting at a certain temperature, carrying out pH value adjustment and cooling on the product after the fermenting, storing for 3-5 days in the fermentation tank, repeating the steps one time, carrying out press filtration on the product to obtain filtrate, and adding the filtrate to a liquid fertilizer to obtain the liquid fertilizer containing the microorganisms. According to the present invention, the natural ecological environment of the soil is simulated, and the three fermentations are performed, such that the fermented microbial agent can quickly adapt to the environment after being put into the soil so as to ensure the survival rate.

Description

technical field [0001] The invention relates to a preparation method of liquid fertilizer containing microorganisms. Background technique [0002] The evaluation indicators for agricultural microorganisms mainly include the purity of the microbial agent and the quantity of the microbial agent per unit mass. Therefore, the preparation method of bacterial agents as the prior art is generally: a fermentation process is carried out under the temperature and pH conditions suitable for microorganisms to survive, which is equivalent to greenhouse cultivation. [0003] Due to the serious soil compaction and acidification, the external temperature is erratic, and the soil environment has its own "indigenous" microorganisms, the microbial agents cultivated in the greenhouse are directly put into the soil, because they cannot adapt to the harsh environment and the competition of "indigenous" microorganisms in the soil. Basically all die, even if there is a small amount of surviving be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/02C05G3/04C05F17/00C12N1/20C12N1/16C12R1/41C12R1/07C05G3/60C05G3/80
CPCC05F5/002C05F17/50C05G3/60C05G3/80C12N1/16C12N1/20C05F11/00C05F11/08Y02W30/40
Inventor 姚继胜纪新强
Owner 纪新强
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