3794 results about "Microbial agent" patented technology

Provided are compositions and methods for selectively reducing the amount of antibiotic resistant and/or virulent bacteria in a mixed bacteria population, or for reducing any other type of unwanted bacteria in a mixed bacteria population. The compositions and methods involve targeting bacteria that are differentiated from other members of the population by at least one unique clustered regularly interspaced short palindromic repeats (CRISPR) targeted DNA sequence. The compositions and methods can be readily adapted to target any bacteria or any bacteria plasmid, or both.

An antimicrobial composition that involves a synergistic mixture in terms of active agents, of a primary antimicrobial agent, such as polyhexamethylene biguanide (PHMB), a secondary antimicrobial agent, and optionally an organic acid against various kinds of microbes is described. Various additional processing aids, such as alcohols and surfactants, may also be incorporated within the mixture. The composition allows one to use a significantly less concentration of individual constituent antimicrobial agents to achieve the same or a better degree of antimicrobial efficacy. The antimicrobial composition can be applied to the surface of almost any kind of substrate material, and can achieve a killing-efficacy of about 3 Log₁₀ reduction in microbes within 30 minutes under ambient conditions.

The invention relates to a compound microbial fertilizer and a method for producing the compound microbial fertilizer. The compound microbial fertilizer comprises the following components by weight: 5-15 parts of inorganic fertilizer, 20-45 parts of organic fertilizer, 4-10 parts of medium trace elements, 10-25 parts of chelating agent and 1-8 parts of microbial agent. The compound microbial fertilizer is rich in organic matters, efficient chelating agent and functional micro-organisms and can improve the physical and chemical properties of soil, increase the utilization rate of the medium trace elements, keep the soil nutrient balance, restrain the occurrence of soil-borne diseases and enhance the crop yield and quality.

The invention discloses a soil heavy mental repairing agent and a preparation method and an application thereof. The soil heavy mental repairing agent is composed of an organic material, a mineral raw material, a carbon material, an inorganic fertilizer and a microbial agent, wherein the viable count of the microbial agent is 1*10<7>-1*10<10>, the content of N, P2O5 and K2O is 8%-15% of the content of total nutrient, the content of the organic material is 20%-40% and pH is 5.5-8.5. The invention also provides an application method of the soil heavy mental repairing agent. The dosage is 80-150kg/mu; the effective heavy metal content of the soil can be effectively reduced; the heavy metal absorption of the crop can be obviously restrained; the crop yield and quality can be improved; the yield and income can be increased; the soil heavy mental repairing agent has significance in promoting social stability, economic growth and environmental diplomacy and guaranteeing ecological safety, food safety and human health.

Antimicrobial compositions having synergistic combinations of octoxyglycerin and at least one other antimicrobial agent in formulations which are more effective than prior art compositions without causing increased irritation to the skin of the average user. In certain embodiments, skin irritation may be minimized by low concentrations of antimicrobials and/or the presence of soothing compounds such as zinc. Preferred embodiments include combinations of octoxyglycerin, a quaternary compound, and at least one other antimicrobial agent. Without being bound to any particular theory, it is hypothesized that the unexpected antimicrobial effectiveness of combinations of octoxyglycerin may result from an enhancement of the permeability of microbes to antimicrobials caused by octoxyglycerin.

A topical composition for treating a cutaneous wound includes whole colostrum or one or more growth factors, as well as a base by which the whole colostrum or one or more growth factors are carried. The topical composition may also include, without limitation, immunomodulators, lactoferrin, enzymes, vitamins, minerals, amino acids, or combinations thereof. Other components may also be included in the topical composition, including, but not limited to, antiseptics, antimicrobial agents, and anesthetics. When applied to a wound, the bioactive topical composition promotes healing of the treated wound.

The invention discloses a microbial agent for treating ammonia-containing waste water and a preparation method thereof. The microbial agent for treating ammonia-containing waste water mainly comprises three bacterial strains of nitrobacter sp, nitrosobacteria nitrosomonas sp., denitrobacter sp., and the contents of the three bacterial strains can be regulated or proportioned according to specificcultivation conditions and product requirements through methods of oriented domestication, and the like. The cultivation method comprises the following steps of: enriching a bacterial source, purifying and amplifying stage by stage for cultivating. The microbial agent can be used for oriented domestication according to a waste water treatment result so as to improve the content of a target bacterial strain. The cultivation method has the advantages of high cultivation efficiency, large production capacity, and the like and is suitable for commercially producing complex microorganisms for treating the ammonia-containing waste water in a large scale. The obtained microbial agent can effectively treat low-COD (Chemical Oxygen Demand) and high-ammonia-nitrogen waste water, such as waste water of catalyst production, and the like.

Polymeric anti-microbial agents produced by substituting the nitrogen atoms in the backbone of ethylenimine polymers are provided. The agents are believed to have low human toxicity while being effective against a variety of pathogens and are useful in applications involving human contact, such as cosmetics, hair care products and textiles, as well as in applications with much less human contact, such as coatings.

An antimicrobial composition that contains an antimicrobial agent and a sugar alcohol is provided. The sugar alcohol is more generally more biocompatible and biodegradable than the antimicrobial agent. In addition, without intending to be limited by theory, it is believed that sugar alcohols increase the attraction of the antimicrobial agent to microorganisms (e.g., the cytoplasmic membrane of bacteria). Because a greater percentage of the antimicrobial agent molecules are brought into contact with the microorganisms, the efficiency of growth inhibition is increased. Thus, the antimicrobial composition provides good efficacy without the need for high levels of an antimicrobial agent.

The invention discloses a salt-tolerant microbial agent and a preparation method thereof. The microbial agent contains staphylococcus cohnii FSND-C, arthrobacter creatinolyticus FDN-1, flavobacterium mizutaii FDN-2, paracoccus denitrificans DN-3 and methylobacterium phyllosphaerae SDN-3. The microbial agent can achieve removal of ammonia nitrogen, total nitrogen and CODcr in the same reactor, has a good wastewater treatment effect, and can achieve short-cut nitrification and denitrification or simultaneous nitrification and denitrification while removing COD. Staphylococcus cohnii FSND-C can utilize various carbon sources, has certain salt tolerance, can be applied to the high-salinity wastewater treatment process, simultaneously can secrete a substance under environmental stimulus to enhance the flocculability of sludge, and further widens the application range of the microbial agent.

Personal care and hygiene formulations for topical application to mucosal surfaces. These formulations include an amphiphilic lipid carrier in the form of a colloidal composition which can include a micellar aggregate or mixed micelles dispersed in a continuous aqueous phase, or an emulsion of lipid droplets suspended in a continuous aqueous phase, and an active agent which is an anti-microbial agent. The lipid carrier has high adhesiveness to mucous membranes such as the soft tissues of the oral cavity. The lipid carrier also has a high load capacity for the active agent to be carried to these tissues. These formulations have the desirable properties of carrying a large amount of active agent for controlled and prolonged release thereof at the desired site, such as mucous membrane surfaces and surrounding tissue. Accordingly, the present invention provides a formulation for oral or topical application including an anti-microbial agent and a lipid. The agent is held by the carrier through a hydrophobic interaction and is released from the carrier in a controlled manner over a prolonged period of time. The lipid is also characterized by having a high adhesive capability towards mucous membrane surfaces. The lipid and the agent are preferably present in a ratio in a range of from about 1:10 to 10:1, more preferably from about 1:5 to about 5:1, and most preferably from about 1:3 to about 3:1 in the formulation.

The invention relates to a soil-improving bio-fertilizer and a preparation method thereof. The soil-improving bio-fertilizer is prepared by mixing the following raw materials in parts by weight: 200-400 parts of grass carbon, 200-400 parts of an organic fertilizer, 100-400 parts of an inorganic fertilizer, 50-200 parts of a porous inorganic matter, 10-30 parts of trace elements, 50-80 parts of clay and 10-20 parts of a compound microbial agent. A plurality of microorganisms with the functions of fixing nitrogen, solubilizing phosphate and solubilizing potassium are added into the fertilizer, so that the nitrogen content of soil can be increased, the utilization ratio of a chemical fertilizer is increased, the soil structure is improved, the crop yield is increased, and the crop quality is enhanced.

A wiper having a controlled release anti-microbial agent therein for providing anti-microbial cleansing of surfaces is provided. The wiper is formed from an absorbent base web to which an anti-microbial formulation is adhered. The formulation includes an anti-microbial agent that is capable of being controllably released from the wiper. In some embodiments, a polymer mixture may be employed to control the rate of release of the anti-microbial agent. Various anti-microbial agents, such as metal ions and organic compounds may be employed.

The invention relates to a method for producing a high-efficiency composite microbial agent by using livestock liquid dung, and belongs to the technical field of environmental microbiology and agricultural science. The method comprises the following steps: diluting livestock liquid dung or livestock feces with water; after hydrolysis and acidification treatment, performing standing and settlement for desilting; mixing a supernatant obtained with a photosynthetic bacterial solution for anaerobic culture; using the mixed solution as a base liquid and stepwise adding lactobacilli, saccharomyces, bacillus thuringienesis and actinomycete into the base liquid in a proportion; and after anaerobic culture and aerobic treatment, finally filling the mixture into a biological fluidized bed for further treatment to obtain the high-efficiency composite microbial agent. The microbial agent is rich in the photosynthetic bacteria, the lactobacilli, the saccharomyces, the actinomycete, the bacillus thuringienesis, azotobacter, nitrobacteria, denitrifying bacteria and other beneficial organisms, and be widely used in agriculture, livestock breeding, fresh water aquaculture, organic sewage treatment, microbial deodorization, and other aspects. The technology is suitable to be used by various livestock farms (raisers) for liquid dung treatment.

α-MSH and other amino acid sequences derived from α-MSH were determined to have antimicrobial influences, including against two major and representative cutaneous and mucosal pathogens; Staphylococcus aureus and Candida albicans, Pharmaceutical compositions useful as antimicrobial agents, including for use in reducing the viability of microbes, reducing the germination of yeasts, killing microbes without reducing the killing of microbes by human neutrophils, for treating inflammation in which there is microbial infection without reducing microbial killing, and for increasing the accumulation of cAMP in microbes are disclosed. The antimicrobial agent is selected from the group consisting of one or more peptides including the amino acid sequence KPV, one or more peptides including the amino acid sequence MEHFRWG, or a biologically functional equivalent of any of the foregoing. The most effective of the peprides were those bearing the C-terminal amino acid sequence of α-MSH. i.e., α-MSH (1-13), (6-13), and (11-13).

A biochar-based coast saline-alkali land improvement conditioner and a preparation method and application thereof are disclosed, the biochar-based coast saline-alkali land improvement conditioner comprises, by mass, 55-60 parts of biomass charcoal, 35-40 parts of humic acid, 2-4 parts of beneficial microbial agent, 1-2 parts of urea and 3-4 parts of a binder; the biochar-based coast saline-alkali land improvement conditioner has the synergistic effect of soil improvement, activation of microorganisms, enhancement of crop saltresistance, the soil improvement helps to improve soil microflora and increase the microbial activity in the soil, microbial metabolites also enhance the crop root saltresistance, crop growth feeds back to accelerate the soil improvement, a virtuous cycle system is formed, and multiplier amplification effect can be achieved compared with a single measure.

Disclosed herein are antimicrobial compositions comprising mixtures of botanical extracts, synthetic antimicrobial agents and essential oils which do not rely solely upon alcohol to produce their antimicrobial effects.

The invention belongs to the technical field of fertilizer, particularly relates to disease-resistant compound biofertilizer special for corn and further relates to a preparation method of the disease-resistant compound biofertilizer special for the corn. The disease-resistant compound biofertilizer special for the corn comprises carbamide, compound microbial agents, bran, chitosan, furfural residues, ammonium sulfate, potassium sulfate, monoammonium phosphate, rape seed cakes, manure, humic acid, sesame seed cakes, microelements, calcium superphosphate and plant ash. The nutrient elements of the disease-resistant compound biofertilizer special for the corn are balanced, microelement compositions are abundant, soil productivity and soil fertility can be improved effectively, addition of compound microbial agents can remarkably restrain main corn diseases occurring universally, the disease-resistant compound biofertilizer special for the corn is long in fertilizer efficiency, corn yield can be improved by 25 to 30 percent effectively, the corn diseases can be prevented and cured effectively, soil can be improved effectively through long-term utilization of the disease-resistant compound biofertilizer, and the disease-resistant compound biofertilizer special for the corn can play a role in the whole growth period of the corn.

The present invention discloses a composition and method of providing ionic forms or compounds of any combination of three metals to produce a product that can be used as a antimicrobial as defined by the U.S. Environmental Protection Agency (EPA) such as a hard surface disinfectant and as a foliar spray or water treatment for the control of various diseases in garden row, field and tree crops, on hard surface areas such as equipment from infected fields or in hospitals, homes, etc. as well as against a wide range of human, plant and animal diseases. Additionally, the composition of the present invention is able to be used to coat and otherwise treat and disinfect food products such as but not limited to fish and shellfish, meat, milk, poultry, eggs and irrigated crops (both food and nonfood crops) as well as non food products as defined in the EPA exemption from tolerance for copper and copper products. The composition may include inerts such as surfactants, detergents and buffers to adjust the pH. The composition remains soluble in water and is useful efficacious against the spread of bacteria, viruses and fungi and other pathogens within a source of water such as a reservoir or pool. Alternatively, the composition may be in aerosolized, misted, vaporized, fogged, humidified forms to produce micronized particles which are able to remain in suspension in the air for long periods of time in order to act on air-borne fungal spores and/or pathogens.

A pharmaceutical composition adapted for use as a spermicide, the composition comprising a gel-microemulsion comprising an oil-in-water microemulsion and a polymeric hydrogel. The gel-microemulsion can be used in a spermicidal method.

Also, a gel-microemulsion pharmaceutical composition adapted for use as a formulation base for additional therapeutic agents. Examples of additional agents include, anti-microbial agents and spermicidal agents. Such gel-microemulsions with additional therapeutic agents can be used in methods for appropriate therapeutic treatment.

Also, a gel-microemulsion pharmaceutical composition that is adapted for use as both a spermicide and formulation base for anti-microbial agents to provide a dual function contraceptive/anti-microbial formulation. Method of using such a composition as a dual function contraceptive/anti-microbial formulation are also included.

This invention relates to a method for coating a medical device comprising the steps of applying to at least a portion of the surface of said medical device, an antimicrobial coating layer and a non-pathogenic bacterial coating layer, wherein the antimicrobial and non-pathogenic bacterial coating layers inhibit the growth of pathogenic bacterial and flngal organisms. The non-pathogenic bacterium used in the bacterial coating layer is resistant to the antimicrobial agent. Furthermore, the non-pathogenic bacterium layer includes at least one of the following: viable whole cells, non-viable whole cells, or cellular structures or extracts. The antimicrobial agent and non-pathogenic bacterium are used to develop a kit comprising these compositions in one container or in separate containers. The kit is used to coat a catheter prior to implantation in a mammal.

The invention provides a special bacterial manure for peanut planting, which comprises the following components in parts by weight: 5-25 parts of a compound microbial agent, 30-65 parts of dreg composite powder, 5-20 parts of starch and 10-25 parts of bentonite, wherein the compound microbial agent is composed of bacillus subtilis agent, a rhodopseudomonas palustris agent, a trichoderma harzianum agent and a paecilomyces lilacinus agent; the mass ratio of the bacillus subtilis agent to the rhodopseudomonas palustris agent to the trichoderma harzianum agent to the paecilomyces lilacinus agent is 5:2:1:2, and the total effective viable count of the compound microbial agent is10 billion cfu/g. The bacterial manure is mainly used as a base fertilizer, and can change the microenvironment around the roots of peanuts to prevent various plant diseases and insect pests while promoting nutrient absorption of the peanuts, so that the yield and quality of the peanuts are improved.

The invention discloses a method for producing a complex micro-ecological preparation with microbial agents and enzyme. According to the action principles and characteristics of every microbial strain, the method adopts scientific combination and adds enzyme preparations and acidifier to enable microorganisms in the product to grow rapidly and take dominance in fermented feed and animal bodies so as to reduce the growth opportunity of miscellaneous bacteria. The preparation comprises the following components: bacillus subtilis, lactobacillus plantarum, candida utilis yeast, lactobacillus acidophilus, protease, amylase, cellulase, xylanase, mannanase, galactosidase, pectinase and citric acid. As all strains of the product adopt liquid deep fermentation culture, the non-polluting property of the product is guaranteed. Liquid microbial agents ensure that the number of bacterial colonies of the product is more than 100 million and the product is free from the pollution of other miscellaneous bacteria, while solid microbial agents ensure that the number of bacterial colonies of the product is more than 1 billion and the proportion of miscellaneous bacteria to effective bacteria is not more than 0.0001. The preparation contains bacillus, lactic acid bacteria and yeast, can apply to the processes of making green-yellow storage feed, fermenting feed, soaking feed, and the like, can be directly added to feed for feeding, and can improve the growth performance of ruminant animals, poultry, pigs, aquatic products and special animals.

The invention discloses a special suspension liquid compound fertilizer for saline-alkali soil and a preparation method of the special suspension liquid compound fertilizer. The compound fertilizer comprises the following raw materials in parts by weight: 0.8-7.2 parts of a nitrogen fertilizer, 0.6-1.1 parts of a boric fertilizer, 0.5-0.8 part of EDTA-Zn, 0.5-0.8 part of EDTA-Mn, 2.0-5.0 parts of humic acid powder, 7.0-14.0 parts of a calcium fertilizer 8.0-16.0 parts of a phosphatic fertilizer, 5.0-9.0 parts of a potash fertilizer, 14.0-19.0 parts of an organic matter, 6.0-9.5 parts of a soil conditioner, 0.8-2.0 parts of a microbial agent, 2.0-4.0 parts of a wetting dispersant, 2.0-4.0 parts of an emulsifier, 1.0-3.0 parts of a thickener, 1.5-3.0 parts of an antifreezing agent and 23-30 parts of water. Through reasonable compounding of various nutritional ingredients, especially an efficient synergistic effect among the humic acid, the soil conditioner and the microbial agent, the physical and chemical properties of the soil are well adjusted, re-accumulation of saline matters is avoided, and nutrient elements required for growth and development are provided for crops, thus better growth of roots of the crop is facilitated, the yield and the quality of the crop are improved, and meanwhile, the element deficiency of the crop can be effectively prevented.

The invention discloses microbial ecological organic fertilizer and a preparation method thereof. The organic chemical fertilizer comprises the following components: edible mushroom dregs, sugarcane dregs, peat soil, oil squeezed waste, filter sludge, livestock and poultry manure and a microbial agent, wherein the microbial agent comprises bacillus subtilis, saccharomycetes, aspergillus niger, guanyloribonuclease, trichoderma, lactobacillus, photosynthetic bacteria, actinomycetes and streptomyces microflavus. The prepared organic fertilizer has advantages of wide raw material source, low cost, high absorption utilization rate, comprehensive nutrients, low dosage, no odor, fast fertilizer effect, various bacteria types, high content, capability of preventing injurious pests, simple preparation method and the like.

Microbicidal formulations are described which are preferably ecologically friendly and non-toxic to mammals, and are highly effective against a broad spectrum of detrimental pathogenic microorganisms. The microbicidal formulation contains complexes having the formula R-M, wherein R is at least one organic chelating moiety and M is at least one metal ion which is microbicidal to at least one microorganism. These complexes can disrupt microorganism activities by penetrating the natural protecting bio-films of such microorganisms through the reaction of the R-group with the organic constituents of these microorganisms while releasing M into their intra-cellular media. Thus, this process exhibits its biocidal activities from the inside-out, contrary to other methods which rely on damaging the protective biofilms. These microbicidal formulations can be diluted in suitable proportions into aqueous systems to produce the desired dosages for each individual case, depending on the level and the severity of the contamination. The microbicidal formulations can be applied by conventional methods, e.g., spraying, soaking, fogging, impregnation, and the like. The formulations can also be used as preservatives, such as for fresh or cut flowers and plants. These microbiocides can also be made as gel or solids in different forms by using techniques available to those skilled in the art.

The embodiments disclose means to inhibit the growth and migration of bacteria, mildew and fungus by applying various coatings which contain an inorganic anti-microbial agent onto metal, glass, and plastic substrates. The substrates are used in the manufacture of components for the interior of refrigerators and freezers, which include wire (100) and glass shelving (200, 300), plastic crisper baskets (500) and other articles within the food storage area. The coatings include the inorganic anti-microbial agent combinet with a matrix resin which are applied by electrostatic powder coating (916), roller coating (810), or robotic spraying (710), and then cured.

Compositions comprising silver dihydrogen citrate in combination with a second antimicrobial compound are provided. The second antimicrobial compound may be quaternary ammonia, an oxidizer or a halogen species, such as chlorine, bromine or iodine. Methods of using the antimicrobial composition provide superior antimicrobial effects.

This invention discloses a solid microbial agent for degrading petroleum pollutants and petroleum products. The solid microbial agent is prepared by: mixing primary seed solutions of Bacillus subtilis and Sphingobacterium multivorum at a volume ratio of (0.5-20):1, fermenting to obtain mixed bacterial fermentation solution, and mixing the mixed bacterial fermentation solution and turfy soil at a weight ratio of (0.1-1):1. The active living bacteria number of Bacillus subtilis is 106-1010/mL, and that of Sphingobacterium multivorum is 107-1012/mL. The solid microbial agent has such advantages as high activity in soil, rapid growth and no secondary pollution to environment, and can be used for biological bioremediation of petroleum polluted soil and petroleum product leakage fields.

The invention relates to an early weaning meat sheep fattening compound biological feed additive, which belongs to the technical field of animals feeding by feed. The feed additive comprises prebiotics consisting of candida utilis, bacillus subtilis and a lactobacillus plantarum microbial agent, non-starch polysaccharide enzyme containing cellulase, xylanase, beta-dextranase, beta-mannase and pectinase and a yeast culture formed by using saccharomyces cerevisiae to completely ferment bean pulp. The additive is specially used for fattening period ration of the early weaning(the weaning lunar age is less than or equal to 2 ages of the moon) meat sheep, and the adding percentage for the full-mixing ration of the fast-fattening meat sheep in 3 to 5 ages of the moon is 1 percent. The additive can provide functional nutrients such as digestive enzyme, B group vitamins and growth promoting factors, and the like which are inadequately produced and insufficient for the early weaning baby sheep and simultaneously contains probiotics for adjusting the micro-ecological environment in intestinal canals, thereby improving the immunity and the healthy level of the baby meat sheep; and after weaning, the daily gaining in weight is more than 10 percent in the prior period and later period of fattening, and the economic benefits are obviously improved.