3214 results about "Microbial agent" patented technology

The invention provides an agricultural source solid waste pollution reduction and carbon reduction synergistic effect calculation method and system. According to the agricultural solid waste pollution reduction and carbon reduction synergistic effect calculation method, a livestock and poultry manure and straw mixture serves as a treatment object, an environmental parameter set of microbial metabolism intensity is obtained, and then a microbial agent feeding acceleration rate and a flora activity attenuation curve are dynamically matched. In the aerobic fermentation stage, through linkage of pile turning operation and a microbial inoculum adding strategy, activity balance of surface and deep flora is maintained, and a microbial inoculum adding time window is corrected in combination with historical data. And establishing a quantitative model with a carbon-nitrogen ratio imbalance threshold value as a constraint, iteratively optimizing a nonlinear association relationship between the inoculant feeding acceleration rate and the pile turning operation frequency, and outputting a full-period synergistic effect evaluation result. According to the technical scheme, the pollution reduction and carbon reduction synergistic effect of agricultural source solid waste treatment is improved, and the dual purposes of resource utilization and environmental protection are achieved.

The invention discloses a bio-organic fertilizer for repairing heavy metal cadmium in soil and a preparation method of the bio-organic fertilizer, and belongs to the technical field of organic fertilizers. The organic fertilizer is prepared from the following raw materials in parts by weight: 7-12 parts of a compound microbial agent, 30-40 parts of modified charcoal, 3-8 parts of sodium alginate, 6-8 parts of a chelating agent and 60-80 parts of decomposed edible fungus residues. The compound microbial agent is prepared from potassium-solubilizing paenibacillus mucilaginosus MSSW02, pseudomonas plecoglossicida and bacillus aryabhattai; the chelating agent is composed of calcium humate and nano-hydroxyapatite according to the mass ratio of 100: (3-5). The multi-component synergistic effect is achieved, the content of heavy metal in soil is effectively reduced, especially the content of heavy metal Cd, meanwhile, rich organic matter is contained, the physical and chemical properties of the soil can be remarkably improved, the water and fertilizer retention capacity of the soil is improved, and crop growth is promoted; the remediation effect on medium-light cadmium polluted farmland soil is good, and the growth promoting effect on crops is remarkable.

The invention discloses cured beef and a preparation method thereof, and belongs to the technical field of fermented food. Comprising the following steps: trimming and dicing beef, adding seasoning spices, and adding a microbial agent for fermentation, the microbial agent is prepared from lactobacillus plantarum NR1-7 and aroma-producing yeast. According to the invention, the cured beef fermented by adding the Chinese prickly ash extracting solution, the lactobacillus plantarum NR1-7 and the aroma-producing yeast is relatively high in viable count, good in color and luster, rich in free amino acid quantity, moderate in protease activity and appropriate in organic acid content, and the pH value is stabilized to be about 5.5. Compared with natural fermentation, the growth and reproduction of inoculated dominant bacteria can accelerate the degradation of beef components and generate rich flavor, the pepper extract can ensure the complete beef structure by weakening the activity of endogenous enzyme, and in addition, the sour taste can be alleviated, the excessive degradation of fat can be relieved, and the fermentation quality of the cured beef can be improved.

The invention provides a special charcoal-based slow-release fertilizer for sandy soil as well as a preparation method and application thereof, and belongs to the technical field of fertilizers. The special charcoal-based slow-release fertilizer for the sandy soil is prepared from amino modified biomass charcoal, urea, urea formaldehyde, monoammonium phosphate, potassium sulfate, polyglutamic acid, fulvic acid, clay, attapulgite, a compound microbial agent and sodium alginate. The compound microbial agent is prepared from halomonas (ACCC 11681), bacillus subtilis (ACCC 60429) and bacillus mucilaginosus (ACCC 10094), and the compound microbial agent is prepared from bacillus subtilis (ACCC 60429) and bacillus mucilaginosus (ACCC 10094). The invention further provides a preparation method and application of the special charcoal-based slow-release fertilizer for the sandy soil. The special charcoal-based slow-release fertilizer for the sandy soil can remarkably improve the water and fertilizer retention capacity of the sandy soil, improve the farmland productivity of the sandy soil, save fertilizer and water input and improve the yield and quality of crops.

The invention relates to the technical field of saline-alkali soil improvement, in particular to a saline-alkali soil treatment and improvement method which comprises the following steps: S1, early-stage investigation and planning; s2, physical improvement; s3, chemical improvement; s4, biological improvement; s5, irrigation improvement; s6, later monitoring and maintenance; according to the saline-alkali soil treatment and improvement method, a traditional single improvement mode is broken through, physical, chemical, biological and engineering technologies are fused, a systematic improvement scheme is formed, and physical improvement effectively improves the soil structure and accelerates salt discharge through land leveling, deep ploughing, upturned soil sunning and concealed pipe laying for drainage; a soil conditioner and an acidic material are precisely applied for chemical improvement, and the soil acidity and alkalinity and ion composition are adjusted; in biological improvement, salt is absorbed by virtue of salt-tolerant plants and microbial agents, and soil microbial communities are improved; a fresh water resource or salt water desalination technology is reasonably utilized for irrigation improvement, and salt leaching and water resource recycling are achieved; through the synergistic effect of multiple means, the salt content of soil can be more efficiently reduced, and the soil structure is improved.

The invention discloses two phosphate solubilizing bacteria and application of microbial agents thereof, and belongs to the technical field of microorganisms. The preservation number of the bacillus cereus Sau.XJ-4 is CCTCC (China Center For Type Culture Collection) NO: M 20242890; the preservation number of the arthrobacter sp. Sau.XJ-5 is CCTCC (China Center for Type Culture Collection) NO: M 20242891. The bacillus cereus Sau.XJ-4 and the bacillus cereus Sau.XJ-5 have the characteristics of saline-alkali resistance, aluminum resistance and the like, insoluble inorganic mineral substances such as tricalcium phosphate can be effectively decomposed, insoluble nutrient elements such as nitrogen, potassium and phosphorus in soil are promoted to be converted into soluble nutrients, the content of available nutrients in the soil is increased, and the yield and the quality are improved; the fertilizer can also relieve aluminum stress of plants and promote growth and development of the plants, and has important significance for fully exerting soil ecological fertility and keeping balance of an agricultural ecological environment.

The invention relates to the technical field of soil conditioners, and particularly discloses an intelligent controlled-release core-shell alginate soil conditioner and a preparation method thereof.The conditioner is of a core-shell-layer three-layer structure and comprises a core layer, a middle shell layer and an outer layer, the three layers account for 30%, 40% and 30% of the total weight respectively, and the core layer mainly comprises improved alginate and nutrients; the middle shell layer mainly comprises chitosan and a microbial preparation; and the outer layer mainly comprises modified charcoal and magnetic nano # imgabs0 #. The preparation method comprises the four steps of core layer preparation, middle shell layer coating, outer layer coating and forming post-treatment, and multiple functions of accurate nutrient supply, efficient heavy metal fixation and soil microenvironment regulation and control are achieved through a core-shell-layer three-layer structure design and a pH / temperature / microorganism triple response mechanism by adopting a coaxial electronic injection and layer-by-layer self-assembly technology; the fertilizer has a remarkable improvement effect on sandy soil and saline-alkali soil, and the organic matter increasing rate reaches 40%.

The invention provides a Kitasatospora xanthophycea JY01 strain and application thereof, the strain is separated from intestinal tracts of spring silkworms, has good disease-resistant and growth-promoting effects, and is specifically expressed in that cellulase, beta-1, 3-glucanase, siderophore and auxin IAA can be secreted; pot experiments show that the biocontrol efficiency of the microbial inoculum to wheat stalk rot is 57.90%, the biocontrol efficiency of the microbial inoculum to corn stalk rot is 46.41%, and the plant height and fresh weight are both significantly increased. The microbial agent is expected to be applied to preparation of biological bacterial fertilizer for effectively preventing and controlling soil-borne fungal diseases.

The invention belongs to the technical field of microorganisms, and provides a growth-promoting P.adami M6 and application thereof, the P.adami M6 is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC No: M20242835, the preservation date is December 18, 2024, the preservation number is CCTCC No: M20242835, the preservation number is CCTCC No: M20242835, the preservation number is CCTCC No: M20242835, and the preservation number is CCTCC No: M20242835. The strain is separated from rhizosphere soil of field planting foxtail millet in the Inner Mongolia red peak area, and is obtained through artificial separation and purification; the strain has the growth promoting characteristics of nitrogen fixation, phosphorus dissolution, IAA production and siderophore production; the M6 fungicide is used for inoculating millet, so that the growth of millet seedlings can be obviously promoted, and the plant height, the root length and the overground part and underground part biomass of the millet are obviously increased; m6 is used as a microbial agent to soak seeds during sowing and irrigate a bacterial liquid once 7 days after sowing, so that the growth effect of millet is best.

The invention relates to the field of saline-alkali soil remediation, and particularly discloses a saline-alkali soil improvement and remediation method which comprises the following steps: step 1, soil pretreatment: performing deep scarification operation on saline-alkali soil at the depth of 40-60cm, and applying desulfurized gypsum particles with the particle size of less than or equal to 5mm at the dosage of 3-5t / ha; step 2, paving a biochar-based composite material: mixing biochar, humic acid and nano-hydroxyapatite according to a mass ratio of 4: 1: 0.5 to form composite particles with the particle size of less than or equal to 2mm, and burying the composite particles into soil in a layered manner according to the dosage of 8-12t / ha; through physical and chemical regulation and control of the biochar-based composite material, biological metabolism activation of the composite microbial agent and dynamic optimization of intelligent water and salt management, a cascade remediation system of material salt resistance-microbial soil improvement-plant salt absorption is constructed, all technical modules are mutually coupled, the salt reduction efficiency is remarkably improved, and the comprehensive utilization rate of the biochar-based composite material is increased. Meanwhile, the soil nutrient circulation capacity is enhanced, and the problems that a traditional single technology is limited in action range and long in remediation period are solved.

The invention discloses a coal gangue abandoned mine ecological restoration water retention technology, and belongs to the technical field of environmental protection. According to the remediation technology, after the surface soil of the mine is loosened, the alkaline waste and the heavy metal adsorbent are firstly added for neutralization and adsorption pretreatment, and then the composite soil conditioner is continuously added to sequentially cover and construct the improved composite layer soil in a gradient manner; the composite soil conditioner comprises fly ash, steel slag, local clay, straw fibers, water-retaining slow-release microcapsules, charcoal, papermaking white mud and humus soil, high-resistance vegetation is transplanted on the obtained improved composite layer soil, meanwhile, a microbial agent is inoculated, and remediation is completed after the soil is maintained to be stable. According to the method, multiple technical processes are integrated and matched and cooperated with one another, the purpose of long-acting water retention is finally achieved through the combined action of multiple remediation mechanisms of materials, vegetation and microorganisms, and compared with a traditional mine remediation technology, the method is better in stability and longer in acting time and has a good application prospect.

The invention discloses talaromyces sp. DFHJ2 and application of a fermentation product of the talaromyces sp. DFHJ2 in strawberry growth promotion and pathogenic bacterium inhibition, and relates to the technical field of microbiology. The talarospora scabra is preserved in the China General Microbiological Culture Collection Center on February 17, 2025, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.41813. The talarospora scabra is named as talarospora scabra. According to the invention, a talaromyces scabra strain is separated from the root of 'Zhongjiang Junjun' strawberry, and the talaromyces scabra strain has a good antagonistic effect on strawberry anthracnose and other types of strawberry pathogenic bacteria which are seriously harmful in the current production, and can be used for efficiently preventing and treating strawberry anthracnose and other strawberry diseases. In addition, the strain is moderate in growth speed, large in sporulation quantity, easy to ferment and high in adaptability to the pH value of the environment, has a certain growth promoting effect on strawberry seedlings, and has the huge potential of being developed into an efficient biocontrol microbial agent.

The invention belongs to the technical field of environment, and particularly relates to an environment-friendly biological agent for repairing and treating petroleum polluted water and soil and a preparation method of the environment-friendly biological agent. The biological agent is prepared by mixing a compound microbial agent, a biological surfactant and an auxiliary material in parts by mass, has the characteristics of high efficiency, environment friendliness and no secondary pollution, can remarkably improve the remediation efficiency of petroleum-contaminated soil, and has a wide application prospect.

The invention belongs to the technical field of agriculture, and particularly provides a compound microbial agent and application thereof. The compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02, and the compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02, and the compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02. The compound microbial agent can effectively improve the pH and EC of saline alkali soil, increase the content of alkali-hydrolyzable nitrogen, available phosphorus and quick-acting potassium in the soil, and play a significant role in increasing the plant height, stem diameter, chlorophyll content and aboveground biomass of corn plants in coastal saline-alkali soil.

The invention relates to the technical field of microorganisms, and discloses a sewage aquatic microbe with ammoxidation capacity, a microbial agent and application of the microbial agent. The strain name of the sewage aquatic microbe is B1, and the preservation number of the sewage aquatic microbe is CCTCC No: M 2025640. The sewage aquatic microbacterium B1 provided by the invention can convert ammonia nitrogen into nitrite nitrogen, so that short-cut nitrification is realized in wastewater treatment, and the strain can tolerate and effectively treat ammonia nitrogen with relatively high concentration, and has relatively good adaptive capacity to environments with different pH values, salinity and temperatures.

The invention relates to a cultivation nutrient medium and a preparation method thereof. According to the nutrient medium, peat is completely abandoned, the coco coir, the straw and the biochar are combined to reduce ecological burden, the nutrient medium is an environment-friendly medium, the biochar fixes carbon and reduces emission, and the soil structure can be improved after long-term use; vermiculite improves the water-holding capacity, perlite and straw form a stable pore structure, and the ventilation porosity reaches 25% or above. Wormcast and a microbial agent synergistically release nitrogen, phosphorus and potassium, and the fertilizer efficiency is lasting. The microbial agent can inhibit soil-borne diseases such as fusarium and reduce the death rate of cultivated seedlings. The biochar adsorbs salt and toxins, and the EC value is stabilized at 1.2-1.8 mS / cm. The cost of the selected raw materials is reduced by 35% compared with that of a traditional substrate, and the substrate is suitable for large-scale production, suitable for vegetable seedling culture, potted flowers, organic farms and soilless culture systems, and especially suitable for saline-alkali soil or continuous cropping obstacle areas.

The invention discloses a compound microbial agent for reducing the cadmium content of brown rice and application of the compound microbial agent. Belongs to the technical field of agricultural microorganisms. The compound microbial agent is prepared from herbaceous spirillum, stenotrophomonas and coxakia, the herbaceous spirillum is herbaceous spirillum R3, and the preservation number of the herbaceous spirillum is CCTCC (China Center For Type Culture Collection) NO: M 20231757; the stenotrophomonas is stenotrophomonas R5, and the preservation number of the stenotrophomonas is CCTCC (China Center For Type Culture Collection) NO: M 20231758; the coxakia is coxakia P1-4, and the preservation number of the coxakia is CCTCC (China Center For Type Culture Collection) NO: M 20231759. Compared with the prior art, the compound microbial agent disclosed by the invention has the beneficial effects that the compound microbial agent disclosed by the invention can reduce the content of cadmium in brown rice, so that farmland soil polluted by light and medium cadmium can be repaired and produced at the same time, and the edible safety of agricultural product rice is ensured.

The invention discloses a rapid restoration method for degraded grassland based on a microbial agent. The microbial agent is formed by compounding 15-30 parts of a nitrogen-fixing microbial agent, 10-20 parts of a phosphate-solubilizing microbial agent, 8-15 parts of a plant growth-promoting microbial agent, 25-40 parts of a humic acid carrier, 3-8 parts of an embedding agent sodium alginate and 5-12 parts of a nutritional agent molasses. Wherein the nitrogen-fixing bacteria are azotobacter chroococcum and alfalfa rhizobium (the viable count is greater than or equal to 5 * 10 < 9 > CFU / g), the phosphate solubilizing bacteria are bacillus megatherium (the viable count is greater than or equal to 1 * 10 < 10 > CFU / g), and the growth-promoting bacteria are bacillus subtilis and pseudomonas fluorescens (the viable count is greater than or equal to 2 * 10 < 10 > CFU / g). The microbial inoculum is subjected to activation, carrier adsorption, sodium alginate embedding granulation and CaCl2 cross-linking processes to be prepared into slow-release particles of 1-3 mm. 200-250 kg of organic fertilizer is applied to each hectare in a mild area, 300-400 kg of organic fertilizer is applied to each hectare in a moderate and severe area, and leymus chinensis and wheatgrass are sown in a mixed mode. After 90 days of application, the vegetation coverage is improved by 40% or above, the soil organic matter is increased by 25-40%, the microbial diversity index is larger than or equal to 3.5, and the comprehensive remediation cost is reduced by 53%.

The invention relates to the technical field of microbial agents, in particular to a composite microbial agent and application of the composite microbial agent in prevention and treatment of forest root diseases, and the composite microbial agent comprises the following components in parts by weight: 15-25 parts of Trichoderma harzianum ATCC 20847; 10 to 20 parts of bacillus subtilis ATCC 605, 10 to 20 parts of bacillus subtilis ATCC 605, 10 to 20 parts of bacillus subtilis ATCC 606 and 10 to 20 parts of bacillus subtilis ATCC 607. 5-10 parts of a seaweed extract; 3 to 8 parts of chitosan; 2-5 parts of humic acid; 1 to 3 parts of glycine betaine; 0.5 to 1.5 parts of a trace element compound; 30 to 40 parts of perlite powder; according to the present invention, the fertilizer has characteristics of significant inhibition effect on a variety of forest root pathogenic bacteria, wide prevention and control spectrum, and long-term and stable disease prevention effect by adjusting the rhizosphere micro-ecosystem. Diseases are prevented and controlled, and the growth and root development of the forest trees are remarkably promoted.

The invention relates to the technical field of microorganisms, in particular to a disease-resistant, growth-promoting and yield-increasing strain and a microbial agent and application thereof. The strain is a strain bacillus velezensis NB21 (CCTCC (China Center for Type Culture Collection) NO: M2024979) which is found autonomously; the strain can grow at low temperature, is strong in ecological environment adaptability, can be used for preparing a single microbial agent or a compound microbial agent, and can also be used for forming a composition with other functional organic and inorganic substances. The prepared microbial agent can significantly prevent and treat diseases, promote root development of crops such as rice and corn, enhance photosynthesis, improve system resistance, increase crop yield, enhance nutrient supply and improve soil micro-ecology. The strain is unique, strong in colonization ability and diversified in function, the prepared microbial agent fermentation production technology is mature, the cost is low, the application range is wide, the yield increasing and quality improving effects are outstanding, and the microbial agent has good application prospects.

The invention relates to the technical field of land treatment, in particular to an organic fertilizer for improving the soil fertility of a sand land. Comprising the following raw materials in parts by weight: 3-12 parts of a microbial agent, 20-50 parts of volcanic rock, 5-12 parts of an additive, 32-45 parts of sodium bentonite, 12-18 parts of a functional agent, 5-25 parts of a modifier and 15-32 parts of a water retention material. By increasing organic matters in the soil, the viscosity of the soil is improved, the air permeability, water retention and permeability of the soil are improved, the formation of a soil aggregate structure is promoted, and the water and fertilizer retention capability of the sand soil is improved.

The invention discloses a bacillus AH83 capable of resisting saline and alkaline and promoting growth and application thereof, and belongs to the technical field of microbiology and biology, the bacillus AH83 is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC No: M 20241495, and the bacillus AH83 is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC No: M 20241495. The AH83 provided by the invention is a new species of bacillus, can inhibit the growth of pathogenic bacteria of cotton fusarium wilt, and has the growth promoting functions of dissolving potassium, generating indoleacetic acid, cellulase, protease, siderophore and the like; meanwhile, the AH83 can survive in a highly saline-alkaline environment, can promote agronomic characters such as plant height, biomass, leaf area and root development in a corn seedling stage when applied in saline-alkaline soil, can also increase soil nutrients and enzyme activity content, and has a promoting effect on lavender seed germination. The strain is of great significance in improving crop yield and stress resistance and developing novel agricultural microbial agents.

The invention relates to a microbial agent prepared based on a new strain of bacillus velezensis, which has the function of broad-spectrum antagonism of common pathogenic bacteria of aquatic products and can effectively prevent and / or treat bacterial diseases and / or fungal diseases of aquatic organisms including crustaceans and fishes. Meanwhile, the content of nitrite and ammonia nitrogen in aquaculture water can be effectively reduced, and the usage amount and residual quantity of antibiotics and chemical drugs in aquaculture are reduced.

The invention relates to the technical field of soil remediation, in particular to a soil ecological remediation agent based on coal gangue-fly ash and a preparation method of the soil ecological remediation agent. The soil ecological restoration agent comprises the following components in parts by weight: 40-50 parts of coal gangue-fly ash mixed powder, 6-12 parts of a microbial agent, 15-20 parts of humic acid, 6-12 parts of sodium polyacrylate, 8-12 parts of straw carbon, 20-25 parts of bentonite and 4-6 parts of aluminum sulfate. The soil ecological restoration agent can supplement trace elements into soil, meanwhile, the coal gangue-fly ash can adsorb heavy metals in the soil so as to fix the heavy metals, the microbial agent can be loaded on the surface of the coal gangue-fly ash, the heavy metals adsorbed on the coal gangue-fly ash are precipitated through the microbial agent, and the heavy metals adsorbed on the coal gangue-fly ash can be fixed. The concentration of heavy metal ions in the soil is reduced.

The invention discloses actinomucor elegans EM-27, a microbial agent and application, and belongs to the technical field of microbial application. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on April 24, 2025, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.41924. According to the invention, an actinomucor elegans strain is separated from wheat rhizosphere, and antagonism experiments find that 8.7% of rhizosphere bacteria have antagonism on the actinomucor elegans, and 91.3% of rhizosphere bacteria and actinomucor elegans can coexist. And by applying actinomucor elegans, the growth and development of wheat can be obviously promoted. Therefore, the actinomucor elegans has a good application prospect in the aspect of improving plant growth and development.

The invention discloses a Chinese chestnut composite phosphate-solubilizing bacterial agent and application thereof, and belongs to the technical field of microorganisms. The invention provides a compound phosphate-solubilizing bacterial agent. The compound phosphate-solubilizing bacterial agent is prepared from phosphate-solubilizing bacteria GXLK-W6 and phosphate-solubilizing fungi GXLK-WZ1-1. The phosphorus-solubilizing bacteria GXLK-WZ1-1 are classified and named as Aspergillus niger, the preservation number of the bacteria is CGMCC (China General Microbiological Culture Collection Center) No.41390, the phosphorus-solubilizing bacteria GXLK-W6 are classified as Burkholderia pyrrolothrix, and the preservation number of the bacteria is CGMCC No.31124. The phosphorus-solubilizing bacteria GXLK-WZ1-1, the phosphorus-solubilizing bacteria GXLK-WZ1-1, the phosphorus-solubilizing bacteria GXLK-WZ1-1, the phosphorus-solubilizing bacteria GXLK-WZ1-1 and the phosphorus-solubilizing bacteria GXLK-WZ1-1 and The strains are independently separated from rhizosphere soil of Chinese chestnuts and are screened. And calcium phytate is added into the microbial inoculum to serve as a slow-release phosphorus source. The compound phosphate-solubilizing bacterial agent improves the growth amplification of Chinese chestnut seedlings, promotes the growth of root systems, and promotes the phosphorus absorption of plants. The compound phosphate-solubilizing bacterial agent can improve the dissolution efficiency of inorganic phosphorus in soil and increase the content of soluble available phosphorus, and plays an important role in improving the fertility of the soil.

The invention relates to the technical field of microbial culture, and provides a culture device for agricultural microbial agent production and a use method, the culture device comprises a culture box, and the top of the culture box is hermetically and detachably provided with a box cover. According to the device, the air pump is controlled by the controller to be started, so that the air pressure in the closed space is increased, the air passes through the non-woven fabric and the polymer microporous membrane, more tiny bubbles can be generated under the action of the polymer microporous membrane, a nutrient solution flows and is mixed, and the stirring effect on the nutrient solution is achieved; a nutrient solution in the incubator flows into the culture dish through the liquid discharge pipe, so that the stirring of the nutrient solution and the injection of the nutrient solution into the culture dish to be mixed with a microbial inoculum can be completed without using more structural components, the operation is simple, and the condition of pollution caused by improper cleaning is avoided; therefore, the subsequent microbial agent culture effect is improved.

The invention relates to a microbial strain and application thereof, in particular to bacillus brevis, a microbial agent prepared from the bacillus brevis and application of the microbial agent. The invention provides the cold-resistant bacillus subtilis which has multiple functions of generating capsular polysaccharide, being high in salt resistance and cold resistance, promoting growth and disease resistance of plants in saline-alkali soil and the like. The bacterial strain can generate capsular polysaccharide, experiments prove that the bacterial strain has high capabilities of dissolving phosphorus, fixing nitrogen and producing siderophore, IAA, ACCD and cytokinin, and has strong antagonistic action on fusarium, curvularia and microplasmodium, and the generated capsular polysaccharide can promote salt tolerance of plants, so that the yield of the plants is increased, and the yield of the plants is increased. The bio-fertilizer is suitable for production of multifunctional bio-fertilizers with disease prevention, growth promotion, salt and alkali resistance, cold resistance and the like, is applied to production of saline-alkali soil grain crops, pasture and greenhouse commercial crops, has remarkable effects of promoting cold damage resistance, root development, prevention and control of root diseases, improvement of crop yield and the like, can reduce the use of chemical fertilizers and pesticides, and has a wide application prospect.

Provided are a quality-improving and nitrogen-fixing coupled microbial agent and the use thereof. The quality-improving and nitrogen-fixing coupled microbial agent is a microbial composition, has coupled functions of improving quality and fixing nitrogen so as to improve the quality of products of leguminous crops, and has the functions of regulating and increasing the rhizobial abundance for the rhizosphere of leguminous crops and increasing the amount of nodules of leguminous crops. The microbial agent contains all gene sequences of nucleotide sequences shown as SEQ ID NO.1-12 or four or more gene sequences among same. When being applied to production of leguminous crops, such as soybeans, peanuts, peas, broad beans and cowpeas, the quality-improving and nitrogen-fixing coupled microbial agent has coupled functions of improving quality and fixing nitrogen and achieves quality improvement and nitrogen fixation and green yield increase. The microbial agent is easy to use, achieves remarkable social and ecological benefits, and facilitates popularization and use.

The invention provides a method for intensively removing antibiotic resistance genes in a composting process, and belongs to the technical field of solid waste treatment. The method comprises the following steps: performing aerobic composting on compost materials, and when the temperature in a compost body is reduced to 38-42 DEG C, inoculating a microbial preparation; the microbial preparation is a microbial agent with a function of promoting degradation of organic matters. Based on the principle that a microbial preparation is inoculated in a compost cooling period (38-42 DEG C), a microbial community structure is changed, and ARGs host bacterium proliferation is effectively inhibited, the effect of strengthening removal of resistance genes in the compost process is remarkable, operation is easy and convenient, control and implementation are easy, secondary pollution cannot be caused in the whole process, and the method is suitable for industrial production. The method has a wide prospect of popularization and application in a large range.