359 results about "Microbial agent" patented technology

This invention relates to the fields of agricultural product processing and bio-feed technology, specifically to a method and application for detoxifying corn steep liquor. The method includes: subjecting a mixture of corn steep liquor, a composite color-protecting agent, and a composite catalyst to a first reaction; allowing the reaction system after the first reaction to stand in a mixture with a composite adsorbent; subjecting the mixture containing the reaction system after the stand-in reaction and a composite enzyme preparation to stand-in treatment, mixing treatment, and enzymatic hydrolysis; and subjecting the mixture containing the reaction system after the enzymatic hydrolysis and an activated composite microbial agent to anaerobic fermentation. This method employs a multi-stage chemical-enzyme-fermentation linkage process, achieving efficient detoxification of corn steep liquor while significantly improving flavor and nutrition. This method synergistically removes mycotoxins and sulfites, and enhances palatability and probiotic value through fermentation transformation of off-flavor substances and enrichment of small peptides and lactic acid. The process is simple and requires no adsorbent separation.

The application provides a bacillus desiccabilis strain XT23 microbial agent, a preparation method and application thereof, and belongs to the technical field of biological agents. A biological agent comprises a bacillus desiccabilis strain XT23, a fermentation treatment liquid of the bacillus desiccabilis strain XT23, or supernatant separated from the fermentation treatment liquid of the bacillus desiccabilis strain XT23. The fermentation treatment liquid is obtained by acid treatment and heat treatment of the fermentation liquid of the bacillus desiccabilis strain XT23, which effectively promotes spore formation, inactivates miscellaneous bacteria, significantly improves the stability and storage period of effective live bacteria, maximally retains antibacterial active substances in the fermentation liquid, and makes the inhibition effect of the final biological agent on Vibrio parahaemolyticus equivalent to that of fresh fermentation liquid. Meanwhile, the addition of the auxiliary material has a protective and slow-release effect on the active ingredients, and further enhances the convenience and effect persistence of application.

The present application relates to the technical field of microbial preparation, in particular to a compound microbial inoculant for selenium-enriched rice, a preparation method and application thereof.The compound microbial inoculant comprises selenium-reducing LYB-S bacterial liquid, bacillus subtilis bacterial liquid, xanthan gum, nano zinc oxide, nano iron oxide and water; wherein, in terms of mass percentage, the addition amount of the selenium-reducing LYB-S bacterial liquid is 50%, the addition amount of the bacillus subtilis bacterial liquid is 25%, the addition amount of the xanthan gum is 0.3%, the addition amount of the nano zinc oxide is 2%, the addition amount of the nano iron oxide is 1%, and the balance is water, and the total mass percentage is 100%. The compound microbial inoculant can be used to increase the selenium content of rice. In addition, the compound microbial inoculant can also promote the growth of rice simultaneously, increase the number of grains per panicle and panicle weight, and realize yield increase.

This invention discloses a compound microbial agent for alleviating continuous cropping obstacles in Scutellaria baicalensis, its preparation method, and its application, belonging to the field of agricultural microbiology. The compound microbial agent comprises halophilic Bacillus and Bacillus belye, and an agriculturally acceptable carrier; wherein, single bacterial suspensions of halophilic Bacillus and Bacillus belye are mixed to obtain a compound bacterial suspension, and the compound bacterial suspension is further compounded with an agriculturally acceptable carrier to obtain the compound microbial agent for alleviating continuous cropping obstacles in Scutellaria baicalensis. The halophilic Bacillus is classified and named... Bacillus halotolerans X14-2, classified as Bacillus belye Bacillus velezensis X147. This invention solves the problems of insufficient adaptability and poor growth-promoting effect caused by the single strain in the microbial agent in the prior art. It has the potential to prepare products that alleviate the continuous cropping obstacles of Scutellaria baicalensis or promote the growth of Scutellaria baicalensis.

This invention relates to a microbial agent with highly efficient plastic degradation properties and its application, wherein the microbial agent contains *Cryptococcus lauriensis* strain with accession number CCTCC NO: M 2026232. Papiliotrema laurentii SNP7-2P strain and / or Achromobacterium with accession number CCTCC NO: M 2026231 Achromobacter strains FJ1-2L. These two microbial strains exhibit varying degrees of degradation effects on long-chain polymers in plastics, effectively and environmentally addressing plastic pollution and making them suitable for preparing green and environmentally friendly degradable plastic products.

This invention discloses a composite microbial agent with both efficient ferulic acid degradation and growth-promoting functions, and its preparation method. The active component of this composite microbial agent contains *Pseudomonas soybeanii* with accession number CGMCC No. 36468. Pseudomonas soyae Strain A9, and *Pseudomonas aeruginosa* with accession number CGMCC No. 36469. Paraburkholderia oxyphila Strain A23. This invention, by screening and combining two functionally complementary strains, fundamentally solves the technical problems of existing single-strain ferulic acid degrading bacteria agents having limited function and unstable field application efficacy, achieving improved degradation efficiency and synergistic growth promotion effects.

The application discloses a method for removing impurities from cow urine and application thereof in preparation of algal culture solution, and relates to the technical field of biological treatment. The method for removing impurities from cow urine adopts a multistage treatment system of chelating agent removal, activated carbon adsorption, physical filtration, composite microbial fermentation and precision filtration; the physical filtration adopts a stainless steel filter screen; the microbial fermentation adopts a composite microbial agent containing bacillus pasteurii, bacillus subtilis and lactobacillus plantarum; and the precision filtration adopts a microporous filter membrane. The application develops an efficient, environment-friendly and low-cost method for removing impurities from cow urine, removes macromolecular contaminant substances and harmful microorganisms and the like impurities in the cow urine, and meanwhile retains beneficial nutrient components in the cow urine; high-quality algal culture solution is prepared through the impurity-removed cow urine, the cost of the algal culture solution is reduced, and the nutrient components in the cow urine are fully utilized, so that abundant nutrient substances are provided for the growth of algae.

This invention discloses the application of a compound microbial agent S14 in improving the salt tolerance and promoting the growth of potatoes, belonging to the field of microbiology. The compound microbial agent S14 is composed of three bacteria: Lelliottia sp. 132, Leucobacter tardus 153, and Pantoea ananatis 103. This compound microbial agent can effectively promote potato growth and significantly increase the emergence rate, biomass, plant height, and tuber weight of potatoes. The compound microbial agent S14 can not only effectively maintain the ion content of hybrid diploid potato seedlings, but also effectively improve the salt tolerance of potatoes, thereby increasing the tuber weight of the commercial variety Fiurita in saline-alkali soil. The compound microbial agent S14 provided by this invention can improve the salt tolerance of potatoes and is suitable for potato cultivation in saline-alkali soil, belonging to the field of plant rhizosphere growth-promoting bacteria technology.

This invention discloses a compound microbial agent, its preparation method, and its application, relating to the fields of soil fertilizer and tobacco cultivation technology. The compound microbial agent comprises fermentation broth of *Bacillus hygroscopicus*, fermentation broth of *Bacillus belye*, and fermentation broth of *Vibrio natans*, with a volume ratio of (0.9~1.1):(0.9~1.1):(0.9~1.1). This compound microbial agent can be applied to tobacco soil improvement. The compound microbial agent provided by this invention has advantages such as excellent properties, controllable application cost, convenient operation, no environmental pollution, economical preparation and fermentation costs, and stable effective live bacteria activity. It can directly replace some conventional chemical fertilizers, directly promote the improvement of soil physicochemical properties, and alleviate soil nutrient imbalance.

This invention relates to a method for efficiently spraying soil conditioner to treat saline-alkali land using composite microorganisms, belonging to the field of soil improvement technology. The method involves screening salt-tolerant functional microbial strains to prepare a composite microbial agent, which is then mixed with carrier materials such as diatomaceous earth, soybean meal, yeast metabolites, and well-rotted farmyard manure in a specific ratio to form a conditioner for application. The conditioner is applied using a two-stage spraying method, combined with deep tillage pretreatment and controlled temperature and humidity conditions, significantly improving microbial activity and soil condition uniformity. Experimental data show that after applying this method, the soil pH value decreased to 7.3-7.5, the salt content decreased to 0.1%-0.2%, and the organic matter content increased to 18-22 g / kg, achieving efficient ecological restoration and sustainable utilization of saline-alkali land.

This invention belongs to the field of microbial technology, specifically relating to a compound microbial fertilizer that synergistically regulates rhizosphere flora and nitrogen metabolism genes and its application. The microbial agent includes: Bacillus belye (… Bacillus velezensis ), Bacillus subtilis subsp. subtilis ( Bacillus subtilis subsp. subtilis ) and Linyi Streptomyces ( Streptomyces linyiensis Two or three of the following. The microbial agent provided by this invention has significant growth-promoting and nitrogen-fixing effects when applied to crops. It can maintain or even improve soil fertility and crop yield under the condition of reducing nitrogen fertilizer application by 30%, providing a green and efficient new solution for the implementation of "nitrogen reduction and efficiency enhancement".

This application relates to the field of biological control, specifically disclosing a compound bacterial agent containing Bacillus thuringiensis, its preparation method, and its application. A method involves fermenting Bacillus thuringiensis HP-SYJ2 to a concentration of (3-8) × 10⁻⁶. 9 A Bacillus thuringiensis HP-SYJ2 bacterial suspension was obtained by mixing cfu / ml with hydroxyapatite and biochar, then mixing the mixture with the Bacillus thuringiensis HP-SYJ2 bacterial suspension, soaking, and air-drying until the moisture content is 30-40%, followed by maturation for 1-2 weeks to obtain the loading material. Fenugreek seed oil, emulsifier, soil penetrant, and water were mixed and sheared to obtain an emulsion with a fenugreek seed oil concentration of 0.1-0.5 wt%. The loading material and emulsion were packaged separately and mixed before application. This compound microbial agent can be used for the prevention and treatment of root rot in medicinal herbs or to promote their growth. It has the advantages of both preventing and treating root rot in medicinal herbs and promoting their growth.

This invention relates to the field of microbial technology, specifically to a compound bacterial agent, its preparation method, and its application, wherein the active ingredient is Bacillus arachidica (B. arachidica). Peanut Bacillus CH-RS16 and cold-resistant *Bacillus subtilis* ( Peribacillus frigoritolerans ZM-RS6. The compound microbial agent provided by this invention promotes the growth and development of wheat and improves its drought resistance.

This invention discloses a pesticide composition for controlling guava nematodes and its preparation method, belonging to the field of pesticide technology. By weight, the raw materials of the pesticide composition include the following components: 8-12 parts of neem seed extract, 8-12 parts of pyrethrum extract, 5-8 parts of tongkat ali extract, 5-8 parts of croton fruit peel extract, 5-8 parts of guava leaf extract, 20-25 parts of compound microbial agent, 3-5 parts of sodium lignosulfonate, 5-8 parts of xanthan gum, 4-6 parts of trehalose, and 80-100 parts of water. The above components work synergistically to achieve excellent control of guava nematodes.

This invention belongs to the field of food processing technology, specifically providing a method for preparing antioxidant zinc chelate peptides, comprising the following steps: inoculating a protein solution with a compound microbial agent for fermentation; the compound microbial agent includes *Lactobacillus plantarum* and *Saccharomyces cerevisiae*; after enzymatic hydrolysis and debittering of the fermentation broth, the antioxidant peptides are separated and dried to obtain the antioxidant peptides; the antioxidant peptides are dissolved in water to prepare a peptide solution, and a zinc source is added to the peptide solution to carry out a chelation reaction; the supernatant is collected, and the precipitate is collected by alcohol precipitation to obtain the antioxidant zinc chelate peptides. This method uses low molecular weight peptides prepared by dual-microbial synergistic multi-enzyme hydrolysis as chelating ligands, which chelate with zinc ions under mild conditions to maximize the preservation of biological activity, achieving a dual enhancement of chelation capacity and antioxidant activity, and achieving a unity of high chelation rate, high antioxidant activity, high stability and good flavor. The process is green, safe, and leaves no organic solvent residue, making it suitable for large-scale industrial production.

The application discloses Paracoccus communis C4-4 and C5-3, the preservation number of which is CGMCC NO:31971 and the classification name of which is Paracoccus communis, and the preservation number of which is CGMCC NO:31972 and the classification name of which is Paracoccus communis. Researches show that C4-4 and C5-3 have strong ecological adaptability and good growth promotion ability, and can improve the internal environment of soil in combination with organic materials. A synthetic microbial population constructed by Paracoccus communis comprises C4-4 and C5-3. Tests prove that the synthetic microbial population comprising C4-4 and C5-3 has the advantages of the synthetic microbial population, the microbial population has high alkali production and growth promotion ability, has good ecological adaptability, the pH adaptation range is 4-8, the manganese stress tolerance can reach 5000 μmol / L, can significantly improve the pH of a culture medium, and has obvious alkalization effect. The synthetic microbial population is used as a microbial agent and is compounded with organic materials to prepare a soil conditioner capable of improving acid soil and promoting plant growth. The conditioner is applied to acid and lean soil, can significantly improve the soil pH, improve the content of soil organic matter, nitrogen, phosphorus and potassium, and promote root growth.

This application relates to the field of feed processing technology, specifically to a quail feed that can improve egg production rate and egg quality, and its preparation method. Specifically, the quail feed, by weight, includes the following components: 50-90 parts feed matrix, 0.5-4 parts eggshell fortifier, 0.1-0.5 parts sodium chloride, 0.5-2 parts vitamins, and 5-10 parts component A, wherein component A includes 0.1-1.2 parts microbial agent, 1-5 parts amino acids, and 2-6 parts sugars. Using the quail feed provided in this application allows quails to maintain good egg production rate and egg quality even in environments with fluctuating temperatures. Compared to existing quail feeds, it has better applicability, and its simple composition makes it easy to prepare, thus possessing significant application value.

This invention discloses a compound bio-fermented feed and its preparation method, belonging to the field of feed processing technology. The compound bio-fermented feed comprises the following raw materials in parts by weight: 55-65 parts of North China camel hair quinoa, 5-10 parts of sea buckthorn pomace, 15-20 parts of corn flour, 3-5 parts of molasses, 0.4-0.8 parts of compound microbial agent, 2.5-3 parts of silica, and 0.5-1 parts of antifungal agent. The compound bio-fermented feed prepared by this invention has excellent antifungal properties, and also has high content of crude protein, amino acids, and other nutrients, low crude fiber content, while adding flavonoid active ingredients, demonstrating good nutritional optimization effects.

The present application belongs to the technical field of plant cultivation, and particularly relates to a biological fertilizer for relieving fruit drop of Taiwan long fruit mulberry and application. The biological fertilizer comprises animal manure, soybean meal powder, humic acid, peat soil, coconut bran, bran, fermentation liquor of a compound microbial agent, seaweed extract powder, amino acid chelated iron and borax; wherein the compound microbial agent comprises Bacillus amyloliquefaciens and Pseudomonas aeruginosa. The above biological fertilizer is balanced in nutrients, is beneficial to enhancing the resistance of the mulberry to adversity and reducing fruit drop caused by stress; in addition, the Bacillus amyloliquefaciens and the Pseudomonas aeruginosa are used synergistically, and the two jointly build a healthy rhizosphere micro-ecology, thereby guaranteeing nutrient supply for fruit development from the root source. Therefore, the technical scheme of the present application can not only significantly reduce the fruit drop rate, but also improve the single fruit weight, soluble solid content and anthocyanin content of mulberry, and improve the flavor and color of the fruit.

This invention belongs to the field of heavy metal removal technology, specifically disclosing an extract from the leaves of a Solanaceae plant, its extraction method, and its application. The extraction method includes the following steps: S1, mixing tomato and pepper leaves, chopping them to obtain a preliminary mixture; S2, mixing a compound microbial agent with the preliminary mixture obtained in S1, fermenting, sterilizing, centrifuging, filtering through a 0.45μm filter membrane, discarding the precipitate, and obtaining a fermented preliminary mixture; S3, concentrating the fermented preliminary mixture obtained in S2 under reduced pressure to obtain a concentrated fermentation broth; S4, adding sodium carboxymethyl cellulose to the concentrated fermentation broth obtained in S3, stirring evenly, and freeze-drying to obtain the Solanaceae plant leaf extract. This Solanaceae plant leaf extract can effectively remove cadmium from aquatic products, aiding in the growth recovery of aquatic products after cadmium stress, and can also effectively maintain the pH value of white clam muscle, reducing lactic acid accumulation and glycogen consumption, and maintaining the freshness of the meat.

This invention relates to the field of crop breeding technology, specifically to a highly efficient breeding method for early-maturing highland barley. The method includes the following steps: parent selection and matching, planting substrate preparation, application of growth regulators, planting management, multi-generation screening, and variety identification. This invention, by screening parents with early-maturing and disease-resistant characteristics, optimizing the planting substrate (adding microbial agents), precisely applying growth regulators, and dynamically controlling the planting environment and nutrient solution, combined with early maturity, disease resistance, and variety identification, efficiently breeds highland barley with a short growth period and strong resistance to stripe rust and powdery mildew. It optimizes plant type and yield structure, improves grain quality, achieves increased yield and genetic stability, and provides technical support for highland barley variety improvement and high-yield, high-quality cultivation.

This invention discloses the application of a salt-tolerant immobilized denitrifying microbial agent in denitrification of low-temperature saline river water. The salt-tolerant immobilized denitrifying microbial agent is placed in a net bag and evenly suspended in the river channel. The salt-tolerant immobilized denitrifying microbial agent uses zeolite as the immobilization carrier, and the immobilized microorganisms are salt-tolerant denitrifying microbial agents enriched and screened from rivers flowing into the sea. The dosage of the salt-tolerant immobilized denitrifying microbial agent is 2 g·L⁻¹. ‑1 The dosage is the mass-to-volume ratio of the immobilized denitrifying bacterial agent to the river water. The low-temperature saline river water is saline river water with nitrate nitrogen as the main pollutant. The temperature of the low-temperature saline river water is 10℃~25℃. During the denitrification process, a carbon source is added to the river water to maintain denitrification efficiency. This invention has demonstrated its feasibility in practical engineering applications through pilot-scale experiments. It can effectively remediate river pollution and solve the problem of severe and difficult-to-remediate water pollution in saline rivers.

This invention belongs to the field of biopesticide technology, specifically relating to a biopesticide for controlling citrus thrips. The biopesticide, used to control citrus thrips, comprises an active ingredient derived from a compound of *Bretschneidera sinensis* powder and methyl salicylate, or a compound of *Bretschneidera sinensis* and ethyl spinosad; wherein the effective bacterial count of the *Bretschneidera sinensis* powder is 2.5 × 10⁻⁶. 8 Spores / g. This invention discovered that *Cladosporium flavum* is effective in killing citrus thrips and can be used as a microbial agent for the control of citrus thrips. Furthermore, this invention combines *Cladosporium flavum* with other existing bioactive ingredients and found that when combined with methyl salicylate or spinosad, it has a synergistic effect on killing citrus thrips. Compared with single-ingredient formulations, it can improve the control effect on citrus thrips, possessing the advantages of being green, safe, and highly efficient, and can provide support for the research and development of biological pesticides for the control of citrus thrips.

The present application belongs to the field of microbial preparation, and discloses a kind of microbial stabilizer, including non-bacillus or its fermentation bacteria liquid, chemical stabilizer, wherein, the non-bacillus includes at least one of Serratia entomophila, Acinetobacter calcoaceticus.The present application also discloses the preparation method of the microbial stabilizer and its application in prolonging the shelf life of microbial agent.The present application studies the microbial stabilizer containing non-bacillus, finds that the microbial stabilizer formed by the suitable collocation of non-bacillus and chemical stabilizer can maintain the stability of viable bacterial concentration for a long time, can prolong the shelf life of microbial product, reduces the need for frequent production and storage, can ensure the supply of product in market more stable, thereby directly reduces the production cost, improves economic benefit, can be used in the field of microbial preparation technology.

This invention belongs to the field of heavy metal pollution remediation technology, and relates to the preparation and application of a cadmium passivating agent for weakly alkaline soil. The weakly alkaline soil cadmium passivating agent mainly comprises the following components by weight: 30-50 parts of a mineral complex, 40-60 parts of a bioactive component, and 5-15 parts of an auxiliary stabilizer. The mineral complex includes natural sepiolite, calcium-based bentonite, and a polyether-polyester diblock copolymer; the bioactive component includes microbial agents, phosphorus-modified biochar, lecithin, and phosphatidylserine; and the auxiliary stabilizer includes nano-hydroxyapatite and a chitosan-grafted polyaspartic acid complex. This weakly alkaline soil cadmium passivating agent exhibits excellent remediation effects on cadmium in contaminated soil, effectively preventing or reducing the entry of cadmium and other heavy metals into the food chain, and has significant market potential.

The invention discloses the preparation and application of a biochar-fixed plant growth-promoting microbial agent. The preparation involves first adding biochar powder to LB liquid culture medium in a specific ratio, then adding a mixed bacterial solution of plant growth-promoting bacteria, and culturing. After centrifugation, the resulting precipitate is freeze-dried to obtain the biochar-fixed plant growth-promoting microbial agent. This invention uses plant waste as a raw material to prepare biochar, which acts as a carrier to enhance the fixation and enrichment of plant growth-promoting bacteria, thereby increasing the activity and stability of these bacteria in the soil. This promotes the growth of plants that hyperaccumulate heavy metals, increases plant biomass, enhances their tolerance to heavy metals, and improves their absorption and translocation effects, achieving rapid and efficient remediation of heavy metal-contaminated soil.

This invention belongs to the field of environmental microbiology and soil remediation technology, specifically relating to a composite microbial agent, its preparation method, and its application in the remediation of soils contaminated with heavy metals and organic matter. The composite microbial agent provided by this invention combines sulfur-modified biochar, a composite functional microbial community, and slow-release nutrients in a specific ratio. The sulfur-modified biochar contains sulfur-containing functional groups such as S=O and C-S, enabling specific adsorption and fixation of heavy metals. The composite functional microbial community consists of organic pollutant-degrading bacteria, plant growth-promoting bacteria, and heavy metal-converting bacteria in a specific ratio of viable bacteria, complementing each other to synergistically complete the degradation of organic matter and the conversion of heavy metals. The slow-release nutrient component provides long-term nutritional support. The preparation method of this composite microbial agent is simple and suitable for the remediation of heavy metal-organic matter contaminated soils in industrial, mining, and chemical sites, offering advantages such as high efficiency, long-term effectiveness, low cost, and environmental friendliness.

The present application relates to the technical field of microorganism, and particularly relates to a microbial composition, a compound microbial agent and application.The microbial composition of the present application comprises Bacillus amyloliquefaciens, Bacillus brevis and Trichoderma harzianum, the microbial composition of the present application can simultaneously prevent and treat rice seed production process rice blast and storage process aspergillus flavus pollution, realize the whole life cycle protection of rice seed from production to storage, solve the problem that different agents need to be used to prevent and treat rice blast and aspergillus flavus pollution in the prior art.In addition, the compound microbial agent containing the microbial composition is safe, efficient, environmentally friendly, can replace chemical pesticides, reduce environmental pollution and pesticide residues, and the preparation method is simple, easy to industrial production, and has wide application prospect.

This invention proposes a Bacillus compound inoculant, its preparation method, and its application, relating to the field of microbial technology. The method of this invention includes the following steps: selecting 18%-25% glucose, 18%-25% yeast extract, 0.3%-0.7% manganese sulfate, 1%-3% potassium dihydrogen phosphate, 1%-3% diammonium citrate, 3%-7% sodium acetate, and 0.1%-0.5% arginine by mass fraction, with the remainder being distilled water, and thoroughly mixing them to prepare a fermentation medium; selecting Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, and Bacillus lentigines, and obtaining a seed culture for inoculation at a constant temperature; mixing the various seed cultures in a ratio of 0.1-0.5:0.1-0.5:95-98:0.1-2:0.1-2 to prepare a compound inoculant, and fermenting to obtain the Bacillus compound inoculant. The compound microbial agent prepared by this invention can not only change the soil nutrient structure and the distribution of microbial communities in the soil, but also change the content of active ingredients in the rhizome of Ligusticum chuanxiong, thereby significantly promoting the growth of Ligusticum chuanxiong rhizome.

This invention relates to the field of biomedical technology, specifically to a subspecies of Bifidobacterium animalis B189 and its applications. The classification name of this strain is... Bifidobacterium animalum subsp . animal The accession number is CGMCC No. 37052. Bifidobacterium animalis B189 not only exhibits significant antibacterial effects against Helicobacter pylori, but also possesses extremely strong acid and bile resistance, making it valuable for the development of microbial agents to inhibit Helicobacter pylori.