1753 results about "Microbial agent" patented technology

The invention belongs to the technical field of agriculture, and particularly provides a compound microbial agent and application thereof. The compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02, and the compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02, and the compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02. The compound microbial agent can effectively improve the pH and EC of saline alkali soil, increase the content of alkali-hydrolyzable nitrogen, available phosphorus and quick-acting potassium in the soil, and play a significant role in increasing the plant height, stem diameter, chlorophyll content and aboveground biomass of corn plants in coastal saline-alkali soil.

The invention discloses a compound microbial agent for reducing the cadmium content of brown rice and application of the compound microbial agent. Belongs to the technical field of agricultural microorganisms. The compound microbial agent is prepared from herbaceous spirillum, stenotrophomonas and coxakia, the herbaceous spirillum is herbaceous spirillum R3, and the preservation number of the herbaceous spirillum is CCTCC (China Center For Type Culture Collection) NO: M 20231757; the stenotrophomonas is stenotrophomonas R5, and the preservation number of the stenotrophomonas is CCTCC (China Center For Type Culture Collection) NO: M 20231758; the coxakia is coxakia P1-4, and the preservation number of the coxakia is CCTCC (China Center For Type Culture Collection) NO: M 20231759. Compared with the prior art, the compound microbial agent disclosed by the invention has the beneficial effects that the compound microbial agent disclosed by the invention can reduce the content of cadmium in brown rice, so that farmland soil polluted by light and medium cadmium can be repaired and produced at the same time, and the edible safety of agricultural product rice is ensured.

The invention discloses a rapid restoration method for degraded grassland based on a microbial agent. The microbial agent is formed by compounding 15-30 parts of a nitrogen-fixing microbial agent, 10-20 parts of a phosphate-solubilizing microbial agent, 8-15 parts of a plant growth-promoting microbial agent, 25-40 parts of a humic acid carrier, 3-8 parts of an embedding agent sodium alginate and 5-12 parts of a nutritional agent molasses. Wherein the nitrogen-fixing bacteria are azotobacter chroococcum and alfalfa rhizobium (the viable count is greater than or equal to 5 * 10 < 9 > CFU / g), the phosphate solubilizing bacteria are bacillus megatherium (the viable count is greater than or equal to 1 * 10 < 10 > CFU / g), and the growth-promoting bacteria are bacillus subtilis and pseudomonas fluorescens (the viable count is greater than or equal to 2 * 10 < 10 > CFU / g). The microbial inoculum is subjected to activation, carrier adsorption, sodium alginate embedding granulation and CaCl2 cross-linking processes to be prepared into slow-release particles of 1-3 mm. 200-250 kg of organic fertilizer is applied to each hectare in a mild area, 300-400 kg of organic fertilizer is applied to each hectare in a moderate and severe area, and leymus chinensis and wheatgrass are sown in a mixed mode. After 90 days of application, the vegetation coverage is improved by 40% or above, the soil organic matter is increased by 25-40%, the microbial diversity index is larger than or equal to 3.5, and the comprehensive remediation cost is reduced by 53%.

The invention discloses a microbial agent and application thereof in preventing and treating rice blast and promoting rice growth, active ingredients of the microbial agent are formed by compounding cold bacillus with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO: 33492 and bacillus velezensis with the preservation number of CGMCC NO: 33493, and the microbial agent aims at solving the synergistic problem of preventing and treating rice blast and promoting rice growth. According to the complex microbial inoculant, through the synergistic interaction between the two strains, the metabolite of the complex microbial inoculant can completely inhibit the spore germination of magnaporthe oryzae; meanwhile, the fungicide can degrade cellulose, obviously promote the growth of plant height, fresh weight and dry weight of rice, and realize the integration of disease prevention and growth promotion. The invention further discloses a preparation method of the fungicide and an application method of the fungicide for preventing and treating rice blast and promoting growth in a root irrigation mode in the three-leaf stage of rice. The microbial agent disclosed by the invention has dual functions of efficiently preventing diseases and stably promoting growth, and an efficient and safe biological solution is provided for green and sustainable development of agriculture.

The invention discloses a microbial agent for improving the yield and quality of strawberries and a preparation method and application thereof, and belongs to the technical field of microorganisms. The microbial agent comprises the following raw materials in parts by weight: 40-50 parts of a compound microbial agent, 0.3-0.5 part of xanthan gum, 1.5-2 parts of glycerol, 4-5 parts of a citric acid-sodium citrate buffer solution, 1-1.5 parts of potassium humate, 0.3-0.5 part of monopotassium phosphate, 0.2-0.3 part of chelated calcium magnesium and the balance of water, wherein the total parts by weight of the raw materials are 100; the compound microbial agent is prepared from arthrobacter Xinjiang, linear Prussella bursa-pastoris and bacillus coagulans. The microbial agent disclosed by the invention can be used for effectively preventing and treating strawberry diseases, including root rot, fusarium wilt, gray mold and anthracnose; through the synergistic effects of hormone secretion, nutrient activation, disease inhibition and the like, the microbial agent comprehensively promotes root development and nutrient absorption of strawberry plants, promotes strawberry growth, remarkably improves the strawberry yield and fruit quality, and meanwhile remarkably reduces the strawberry storage rotting rate.

The invention discloses a Vinck Cellulomicrobe and application thereof in degradation of corn straw and improvement of soil fertility, and belongs to the field of agricultural microbial technology and solid waste resource utilization. The bacterial strain can secrete a plurality of hydrolase and oxidase including AA10 family lytic polysaccharide monooxygenase, AA2 peroxidase, GH3 and GH1 beta-glucosidase, GH16 / GH17 beta-glucosan endonuclease and AA1 multi-copper oxidase. The bacterial strain has the advantages that the bacterial strain can secrete a plurality of hydrolase and oxidase; fermentation liquor is applied to soil according to the straw mass ratio of 1: 5, and the corn straw degradation rate can be increased under the condition that the mass ratio of soil to straw is 100: 1. Meanwhile, the microbial agent can synergistically improve soil nutrients and increase the contents of rapidly available phosphorus, rapidly available potassium, alkali-hydrolyzable nitrogen, soluble organic carbon and total phosphorus in soil. According to the microbial agent, a straw lignocellulose structure is effectively cracked through a multi-enzyme system synergistic effect, and the dual effects of accelerating straw degradation and improving soil fertility are achieved.

The invention belongs to the technical field of agricultural microorganisms, and discloses a strain of stenotrophomonas sp., which is named as stenotrophomonas sp. R5, is preserved in the China Center for Type Culture Collection on September 20, 2023, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 20231758. The invention further discloses a microbial agent containing the bacterial strain and application of the microbial agent in prevention and treatment of rice planthoppers. According to the stenotrophomonas sp. R5 and the microbial agent containing the stenotrophomonas sp. R5, the control effect on rice planthoppers can reach 90% or above, compared with a conventional control method, the control effect is remarkably improved, and the survival rate of rice is greatly increased.

The invention discloses a microbial agent and application thereof in agricultural production. The microbial agent is prepared by compounding seed solutions of two or more of seed solutions of Trichoderma harzianum, Trichoderma brevicompactum Tb12, Gliocladium roseum NF-06 and Trichoderma asperellum, and the seed solutions of the two or more of the Trichoderma harzianum, the Trichoderma brevicompactum Tb12, the Gliocladium roseum NF-06, the Trichoderma asperellum, the Trichoderma harzianum, the Trichoderma brevicompactum, the Gliocladium roseum NF-06 and the Trichoderma asperellum. The selected strain combination can effectively prevent and treat fungal diseases and nematode diseases and can promote plant growth. The trichoderma harzianum and the trichoderma brevicompactum Tb12 are used in a combined mode, the control effect on fusarium is obvious, the gliocladium roseum NF-06 and the trichoderma asperellum are used in a combined mode, the nematode killing effect is obvious, and the growth of rice is promoted through the trichoderma harzianum and the trichoderma asperellum.

The invention discloses a humic acid type biological organic fertilizer and a preparation method thereof, and belongs to the technical field of agricultural biologication.The fertilizer is prepared from weathered coal humic acid raw powder, seaweed processing residues and insect manure as main raw materials. The soil conditioner is prepared by adding a compound microbial agent, a mineral synergist, an enzyme preparation, a coating material, a soil structure regulator, a signal molecule inducer, a trace rare earth element additive, a stabilizer and the like in a specific ratio and adopting a two-step fermentation process. The fertilizer disclosed by the invention has remarkable functions of soil improvement, nutrient slow release, microbial community regulation and control, crop stress resistance enhancement and the like, remarkably improves the yield and quality of crops, reduces the use of chemical fertilizers, promotes agricultural sustainable development, and has remarkable economic and ecological benefits.

The invention discloses a salt-tolerant brevibacillus for promoting growth and preventing and controlling plant diseases and application of the salt-tolerant brevibacillus. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025, and the preservation number of the brevibacillus halotolerans T101 is GDMCC No: 66731. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025. The strain T101 disclosed by the invention can effectively antagonize plant pathogenic fungi peronophythora litchii and plant pathogenic bacteria such as pseudomonas syringae, xanthomonas campestris, citrus canker pathogen and ralstonia solanacearum, can generate siderophores and plant growth hormone IAA, and can remarkably promote the growth of tomato root systems in a pot experiment. Therefore, the strain T101 can be used for preparing microbial preparation products for preventing and treating plant diseases and promoting plant root growth, and has important application potential in the aspects of microbial agents, biological fertilizers and the like for promoting plant growth and development.

The invention belongs to the technical field of environmental microorganisms, and particularly relates to a microbacterium strain and preparation and application of a microbial agent of the microbacterium strain. The invention discloses a strain of Microbacterium zhangzhouensis GSS18, which is preserved in Guangdong Microbial Culture Collection Center on November 14, 2022, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) No.62978. The strain of Microbacterium zhangzhouensis GSS18 disclosed by the invention has the advantages that the preservation number is CGMCC No.62978; compared with the prior art, the Microbacterium zhangzhouensis GSS18 disclosed by the invention can secrete esterase and lipoid esterase, and can be used for accelerating the rapid conversion of lipid substances of the compost and promoting the compost humus process.

The invention discloses a flora prediction and petroleum pollution remediation method based on machine learning. The method comprises the following steps: collecting multiple groups of experimental data of a diesel oil pollution sample treated by a microbial agent, extracting environmental factors, microbial community characteristics and target response variables to construct a training data set after missing value processing, abnormal value detection and standardized pretreatment, and importing the data set into a preset machine learning model to obtain a training result; carrying out feature learning, classification training and hyper-parameter optimization by adopting a GridSearchCV method in combination with 10-fold cross validation; evaluating the correlation between a target response variable classification result and the features through a multivariable Pearson's correlation matrix, and constructing an optimal test set; and finally, selecting an optimal prediction model according to a preset index. According to the method, the model training quality is improved through data preprocessing and correlation analysis, efficient flora prediction and algorithm application evaluation are achieved by means of multiple machine learning algorithms, scientific support is provided for petroleum pollution remediation, and remediation accuracy and efficiency are improved.

The invention belongs to the technical field of soil organic matter improvers, and discloses a soil organic matter improver based on a compound microbial agent and a preparation method thereof, and the soil organic matter improver comprises the following components: a compound enzyme, a modified chitosan microsphere complex, a modified polyelectrolyte, an inhibitor L-methionine sulfoximine, a substrate, an auxiliary factor and the compound microbial agent. A compound enzyme system is used as a core, soil ammonium ions are circularly and efficiently assimilated through glutamine synthetase / glutamate synthetase, and the soil ammonium ions are converted into organic nitrogen; and energy guarantee is provided by virtue of polyphosphate kinase. The modified chitosan microsphere complex is used for protecting and slowly releasing enzyme and a substrate, and controlled release of the inhibitor L-methionine sulfoximine is realized through the modified polyelectrolyte, so that carbon-nitrogen balance of soil is regulated and controlled. The system has a synergistic effect with a compound microbial agent, the problem of soil organic matter reduction caused by excessive nitrogen application is effectively solved, meanwhile, the crop root biomass is remarkably increased, and the soil organic matter content is increased.

The invention relates to the technical field of microorganisms, in particular to a complex microbial inoculant for resisting salmonella infection and application of the complex microbial inoculant. The invention provides a microbial composition for resisting salmonella infection. The microbial composition comprises lactobacillus reuteri, lactobacillus mucosa, lactobacillus plantarum, lactobacillus bulgaricus and pediococcus pentosaceus. Results of the embodiment of the invention show that the complex microbial inoculant provided by the invention has an excellent anti-salmonella typhimurium effect, and only the anti-salmonella typhimurium effect of the complex microbial inoculant in a specific proportion provided by the invention is optimal; meanwhile, the complex microbial inoculant can effectively relieve the harm of salmonella typhimurium to chickens, improve the growth performance of the chickens and improve the immunity of the chickens. The invention provides a new prevention and treatment thought for salmonella.

The invention relates to the technical field of agricultural cultivation, in particular to a farmland soil ecological structure restoration and soil fertility improvement cultivation method which comprises the steps that 1, the soil state is evaluated; 2, tillage depth is determined; step 3, adding organic materials; 4, tillage operation is carried out, a tillage machine is used for carrying out soil tillage, the tillage depth is the tillage depth determined in the step 2, and the tillage operation adopts a layered tillage mode including shallow tillage and deep tillage; step 5, inoculating a microbial agent, namely inoculating the soil microbial agent based on the soil microbial diversity and the soil enzyme activity in the step 1 after the cultivation operation; 6, covering and maintaining are conducted, after cultivation and inoculation, soil covering and maintaining are conducted, and using of covering materials and precise water and fertilizer management are included. Accurate soil evaluation and personalized tillage depth determination enable soil remediation to be more targeted and effective; organic materials and microorganisms are reasonably matched and used, so that the soil fertility and the biological activity are improved.

The invention discloses compost turning and mixing equipment for organic fertilizer production, and relates to the technical field of organic fertilizer production, the compost turning and mixing equipment specifically comprises a chassis, a lifting conveyor is mounted at the front end of the chassis, and a screw conveyor, a storage assembly and a mixing bin are mounted at the rear end of the chassis; material receiving hoppers are mounted at the feeding ends of the two groups of spiral conveyors; the material storage assembly and the material mixing bin are both located between the two sets of spiral conveyors, and a material mixing mechanism is installed in the material mixing bin. Fertilizer turning and auxiliary material adding and mixing are integrated, the fertilizer does not need to be laterally thrown, the requirement for the site area is relatively low, the fertilizer can make full contact with air in the whole turning process, fertilizer aeration is sufficient, and therefore the compost quality is improved, and according to the actual requirement in the turning process, the fertilizer can be conveniently and rapidly turned. The materials for adjusting the carbon-nitrogen ratio and the moisture and the microbial agents for adjusting the microbial fermentation flora are added in an auxiliary mode, manual adding along with equipment is not needed, and the labor intensity of workers can be greatly reduced.

The invention discloses a compound bacterial agent for reducing blood sugar and blood fat, application of the compound bacterial agent and a method for preparing a fermented coconut milk beverage from the compound bacterial agent, belongs to the technical field of microorganisms, and particularly relates to the compound bacterial agent for reducing blood sugar and blood fat, the compound bacterial agent comprises fermented lactobacillus mucus AL-8 and lactobacillus paracasei DX-5, and the compound bacterial agent is prepared from the fermented lactobacillus mucus AL-8, the lactobacillus paracasei DX-5, the lactobacillus paracasei DX-5, the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5. The preservation number of the lactobacillus mucus AL-8 is CCTCC (China Center For Type Culture Collection) NO: M20252735; the preservation number of the lactobacillus paracasei DX-5 is CCTCC (China Center For Type Culture Collection) NO: M20252736. According to the invention, the fermented coconut milk beverage is prepared by utilizing the inhibition effect of a blood sugar and blood fat reducing compound bacterial agent consisting of fermented lactobacillus mucus AL-8 and casei paracasei DX-5 on alpha-amylase and pancreatic lipase.

The invention relates to the technical field of microorganisms, and discloses Ackermann muciniphila, a combined microbial agent thereof and application of the Ackermann muciniphila in inflammatory enteritis. The invention provides Akkermansia muciniphila AIML-559, and the preservation number of the Akkermansia muciniphila AIML-559 is CGMCC (China General Microbiological Culture Collection Center) No. 34246. The invention relates to a combined microbial agent, which is characterized by comprising Ackermann muciniphila AIML-559 and Bifidobacterium adolescentis AIML-528 (the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.34300). The combined microbial agent is prepared from the following raw materials: Ackermann muciniphila AIML-559 and Bifidobacterium adolescentis AIML-528 The ackermann mucophil combined microbial inoculum is suitable for treatment intervention of inflammatory bowel disease (IBD), and can relieve weight loss, hemolysis, anemia, diarrhea, intestinal bleeding and other symptoms of IBD mice.

The invention belongs to the technical field of agricultural microorganisms, and discloses a bacillus flexus strain BJ699 and application thereof in strawberry production. Specifically, the invention provides a novel bacillus flexus strain BJ699, and the preservation number of the strain BJ699 in the China Center for Type Culture Collection is CCTCC (China Center for Type Culture Collection) NO: M20252013. The invention also provides a microbial agent containing the strain BJ699 as an active component and application of the strain BJ699 in preparation of a microbial agent for prevention and control of strawberry root rot pathogenic bacteria and / or degradation of pyraclostrobin. The new strain or the microbial agent containing the strain comes from the natural environment, has no cross resistance to existing bactericides and insecticides, has the advantages of high efficiency, low toxicity, no environmental pollution, low possibility of drug resistance generation and the like, and has a wide application prospect.

The invention relates to the technical field of wastewater treatment microorganisms, in particular to an efficient compound microbial agent for municipal sewage treatment and application of the efficient compound microbial agent. The compound microbial agent is prepared from the following strains in percentage by mass: 22.5 to 24.3 percent of anaerobic rope bacteria, 10.5 to 13.1 percent of outer bacillus, 6.8 to 7.3 percent of brevibacillus, 3.1 to 4.3 percent of bacillus, 2.3 to 2.5 percent of enterobacter, 1.8 to 2.4 percent of hyphomonas, 2.1 to 2.5 percent of spirillum rot, 1.8 to 2.1 percent of coxiella and 16.8 to 18.2 percent of other bacteria. Aiming at the problems that the existing municipal sewage pollutant removal efficiency is low, and microorganisms are difficult to adapt to water quality fluctuation, the compound microbial agent can adapt to water quality and water quantity fluctuation, and the treatment effect is stable; the COD (Chemical Oxygen Demand) degradation and nitrogen and phosphorus removal capabilities are excellent, and the metabolic capability is strong.

The invention belongs to the technical field of food processing, and relates to a method for preparing low-salt shrimp juice through rapid microbial fermentation. The method comprises the following steps: removing heads of shrimps, preparing meat paste, drying and crushing shrimp shells, and mixing the shrimp shells with the meat paste; carrying out enzymolysis treatment on the meat paste by using alkaline protease, and then carrying out high-temperature enzyme deactivation; mixing the shrimp meat and shrimp shell enzymatic hydrolysate mixture with soybean meal and bran, and sterilizing at high temperature; inoculating a complex microbial inoculant containing lactobacillus plantarum, saccharomyces rouxii, aspergillus oryzae, deep-sea bacillus subtilis and bacillus subtilis to prepare a mold starter, fermenting at 28 DEG C for 30 days, and finally filtering and sterilizing. According to the method disclosed by the invention, the availability of the raw materials is effectively improved, the fermentation period is shortened, efficient fermentation under a low-salt condition is realized, various basic indexes of the obtained shrimp juice product meet national standards, and the shrimp juice product is outstanding in flavor quality and has rich sauce flavor and sweet taste. In addition, the shrimp juice shows good antioxidant stability before and after digestion.

The invention provides a biological organic fertilizer and a preparation method thereof. The biological organic fertilizer is prepared from the following raw materials in parts by weight: 10 to 15 parts of rice husk, 8 to 12 parts of peanut shell, 10 to 15 parts of bagasse, 15 to 20 parts of straw, 20 to 25 parts of livestock manure, 5 to 10 parts of pond sludge, 3 to 5 parts of plant ash, 5 to 10 parts of oil meal, 5 to 10 parts of ammonium phosphate, 2 to 5 parts of low-temperature complex microbial inoculants, 3 to 6 parts of seaweed oligosaccharide, 5 to 10 parts of modified vermiculite powder and 10 to 20 parts of photothermal conversion microspheres. According to the invention, waste is taken as a basic raw material and is matched with ammonium phosphate to rapidly supplement available nutrients, and meanwhile, the low-temperature complex microbial inoculant, the modified vermiculite powder and the photothermal conversion microspheres are introduced to form a synergistic interaction system. Vermiculite powder is modified, so that the nutrient adsorption and slow release capability is improved, a carbon source and low-temperature protection are provided for microorganisms, and the cell membrane stability is enhanced. The photothermal conversion microspheres efficiently absorb sunlight and convert the sunlight into heat energy, a local and mild heat island effect is formed around the fungicide, the microenvironment temperature is increased, breeding of the fungicide is remarkably accelerated, and therefore organic matter decomposition and nitrogen conversion are accelerated.

The invention belongs to the technical field of environmental engineering, and discloses a microbial agent for degrading malathion as well as a preparation method and application of the microbial agent. The microbial agent comprises the following raw materials: compound bacteria, a nutritional supplement and a biomass carrier material, wherein the compound bacteria comprise functional bacteria and synergistic bacteria, and the mass ratio of the functional bacteria to the synergistic bacteria is (1.2-1.8): 1; the functional bacteria comprise issatchenkia orientalis, nocardia, brevibacillus laterosporus and bacillus cereus; the synergistic bacteria comprise azospirillum brasilense, bacillus thuringiensis and lactobacillus buchneri. Functional bacteria with direct degradation capacity and synergistic bacteria with auxiliary functions are creatively compounded according to a specific proportion, meanwhile, the garlic straw powder is selected as a biomass carrier material, all the raw materials complement each other in function and have a synergistic effect, the degradation rate and thoroughness of malathion are remarkably improved, and the degradation efficiency of malathion is greatly improved. The defects of low degradation efficiency and poor environmental adaptability of a single strain are overcome.

The invention relates to the technical field of soil treatment, in particular to an integrated saline grainfield soil improvement and remediation method. According to the method, precise irrigation, concealed pipe drainage, salt-tolerant crop planting and bioremediation technologies are comprehensively applied, and systematic treatment of the salinized soil is achieved. Precise irrigation dynamically regulates and controls the leaching depth and frequency on the basis of real-time soil salinity and moisture data, the underground water level is intelligently controlled in combination with an underground pipe drainage network, salt accumulation is effectively reduced, and water resource waste is avoided; according to the salt-tolerant crop and green manure rotation mode, salt damage is reduced through plant salt absorption and root system nitrogen fixation, soil organic matter accumulation and microbial community reconstruction are promoted through application of a microbial agent and biochar, and a sustainable soil improvement mechanism is formed. The intelligent monitoring system integrates multiple types of sensors, collects key indexes such as salinity, nutrients and pH values in real time, predicts the salinization trend and regulates and controls irrigation, fertilization and drainage equipment in a linkage mode, full-process automatic management is achieved, and the management precision and efficiency are remarkably improved.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to rice endophytic sphingosine vector SY02 and application thereof. The preservation number of the rice endophytic sphingobium yanoikuyae SY02 provided by the invention is CCTCC (China Center For Type Culture Collection) NO: M2026182, and the sphingobium yanoikuyae SY02 has the functions of producing indoleacetic acid, promoting plant growth and seed germination, promoting adventitious bud proliferation of tissue culture seedlings and relieving browning. The sphingosine vector bacterium and the microbial agent prepared through strain fermentation have the advantages of being green, safe, efficient, long-acting and the like, and have good application prospects in the aspects of promoting plant growth and seedling breeding.

The invention relates to a composite microbial fertilizer with a saline-alkali soil improvement effect and a preparation method thereof, and belongs to the technical field of microbial fertilizer preparation, the fertilizer comprises the following raw materials by mass: 10-30 parts of modified charcoal, 5-8 parts of a microbial agent, 30-50 parts of an inorganic fertilizer, 10-20 parts of a soil improvement agent and 3-5 parts of other auxiliary agents; wherein the modified biochar is obtained by performing segmented heat treatment modification on animal husbandry wastes; the microbial agent is obtained by immobilizing compound microorganisms by using sodium alginate gel reinforced by a mineral filler and micron-sized charcoal powder. The prepared fertilizer has the effects of improving crop quality and crop yield and improving saline-alkali soil.

The invention discloses a compound fertilizer for improving the utilization rate of nutrient elements and a preparation method thereof, and belongs to the technical field of organic fertilizer and microbial fertilizer manufacturing. The compound fertilizer comprises the following components in parts by mass: 10-30 parts of a nitrogen source, 5-20 parts of a phosphorus source, 10-25 parts of a potassium source, 3-15 parts of a synergist, 1-5 parts of medium trace elements, 2-8 parts of a coating material and 8-15 parts of a plant growth promoter. The plant growth promoter is a specific deuterated benzene ring derivative and has the structural characteristics as shown in formula 1. The preparation method comprises the processes of raw material mixing and crushing, microbial pre-fermentation, coating liquid granulation, fluidized bed coating and the like. Through chelation of humic acid, activation of a microbial agent and synergism of a deuterated molecular structure, the utilization rate of nitrogen, phosphorus and potassium is increased to 1.5 times or more of that of a conventional fertilizer, field tests show that the yield of purple cabbage can reach 4083 kg / mu, the yield is increased by 31.4% compared with that of a traditional compound fertilizer, meanwhile, the soil microbial environment is improved, and intelligent slow release of nutrients is achieved.

The invention relates to the technical field of tailing sand treatment, in particular to a method for repairing vanadium titano-magnetite tailing sand by utilizing plants, which comprises the following steps: ploughing and flattening the surface layer of the tailing sand, then applying an organic modifier and a pH regulator, and fully mixing with the tailing sand of 20-40cm on the surface layer; synchronously applying a microbial agent containing heteroform rhizopus in the plant growth substrate, and sowing bermuda grass; performing field management after the cynodon dactylon seedlings survive; after at least one complete growth cycle of the cynodon dactylon is completed and the cynodon dactylon enters a mature period, stubbles of 5-10 cm are reserved by using a harvesting machine for cradling so as to ensure that the cynodon dactylon keeps necessary growing points, so that the cynodon dactylon can be quickly regenerated in the next growing season, and then the cradled cynodon dactylon is treated. The phytoremediation efficiency of the vanadium titano-magnetite tailing sand can be improved, and ecological restoration, resource circulation and environmental safety are also considered.

The invention discloses a complex microbial inoculant containing streptomyces elongisporus and trichoderma Guizhou, the streptomyces elongisporus is streptomyces elongisporus A25, and the trichoderma Guizhou is trichoderma Guizhou NJAU4742. The invention further discloses application of the complex microbial inoculant to promotion of cucumber growth and application to promotion of seedless litchi yield improvement and fruit quality improvement. Streptomyces elongisporus A25 and trichoderma Guizhou NJAU4742 which are separated and screened from seedless litchi tree rhizosphere soil are prepared into the composite microbial agent, the composite microbial agent is applied to a cucumber pot experiment, cucumber growth can be remarkably promoted, and when the composite microbial agent is applied to a seedless litchi field experiment, seedless litchi yield improvement and fruit quality improvement can be remarkably promoted. The complex microbial inoculant disclosed by the invention has important significance on sustainable development of the seedless litchi industry.

The invention provides a lake water body purification and ecological restoration method based on a biotechnology. The method comprises the following steps: S1, collecting and analyzing basic data of a lake water body; s2, screening and culturing dominant microorganisms; s3, making an ecological restoration scheme; s4, putting a microbial agent and planting aquatic plants; s5, monitoring the water body remediation process; and S6, repairing effect evaluation and scheme adjustment. According to the method, the degradation efficiency and the environmental adaptability to target pollutants are remarkably improved, an efficient functional microbial agent is provided for precise repair, a microbial culture optimization model is constructed by utilizing a dynamic programming algorithm, and a nutrient substance adding strategy and a culture period are precisely regulated and controlled in combination with the growth dynamic characteristics of strains. On the premise of ensuring the concentration and activity of the microbial inoculum, the resource consumption and time cost in the culture process are effectively reduced, the economical efficiency and reliability of microbial inoculum production are improved, and a foundation is laid for large-scale engineering application.