4 results about "Decreased Sensitivity" patented technology

The invention discloses a leakage-proof peritoneal drainage bag, and relates to the technical field of medical peritoneal drainage, the leakage-proof peritoneal drainage bag comprises a connecting assembly, and the connecting assembly comprises a connecting piece, an inclined piece and a plugging piece; the connecting piece comprises a communicating pipe; the inclined part comprises a mounting plate, a rotating ball, an abutting block and a balancing weight; the plugging piece comprises a positioning plate and a lifting plate; the mounting plate and the positioning plate are fixed in the communicating pipe, and the lifting plate is arranged below the positioning plate in a sliding manner; the abutting block and the balancing weight are fixed to the upper end and the lower end of the rotating ball respectively. The rotating ball can rotate in the rotating opening, the motion resistance is reduced, the plugging response is more sensitive and reliable, and the problems of sensitivity reduction and clamping stagnation caused by friction are avoided; and through the weight difference and shape design of the balancing weight and the abutting block, the device can gradually push the lifting plate to move upwards according to the inclination angle. During slight shaking such as walking and slight body position change, complete plugging is avoided, and normal drainage can be achieved.

The utility model provides a high sensitivity flow type antibody quantitative detection suite, relates to medical supplies technical field, including test paper, solution bag, dipping cotton swab, anti-collision box and quantitation subassembly, dipping cotton swab middle is equipped with the middle folding groove, quantitation subassembly includes test tube and cambered surface piece, cambered surface piece bottom is fixed with the fixed rubber ring, and the fixed rubber ring is fixed in the middle folding groove. A mode of installing a pressing rubber ring is adopted to solve the problem of flow antibody detection, which is an advanced immunoassay technology, a common user can also carry out self-test at home, the test suite generally comprises all necessary materials and instructions, so that the user can collect and analyze a blood sample through simple steps, but under the condition of no professional guidance, the test suite has the advantages of simple operation and low cost. In the prior art, the risk of operation errors exists, a user is difficult to master the dropping amount of a solution to test paper after sampling blood and dropping the blood into a medical solvent, if the dropping amount of the blood is too small or too large, the reagent reaction is incomplete, the final detection result is influenced, the test result is inaccurate, and the sensitivity is reduced.

The invention discloses a method for detecting an mRNA capping rate, and belongs to the technical field of biology. The detection method comprises the following steps: carrying out hybridization reaction on mRNA and a base-free modified probe, and carrying out enzyme digestion reaction on the product; and adding magnetic particles into the reaction product, incubating, cleaning and eluting to obtain the mRNA pretreatment product. According to the method, the unmodified probe is used for detecting the cap adding rate of the mRNA, the pretreatment process of the mRNA is mainly improved, and the reaction condition of hybridization combination of the probe and the mRNA is optimized, so that the higher detection fragment yield is obtained; by optimizing the elution mode, the proportion of the probe in a capping rate detection sample can be remarkably reduced, and the proportion of a target fragment is increased. Therefore, compared with a traditional detection method, the method has the advantages that the detection sensitivity can be improved, the detection lower limit is reduced, the resolution and accuracy are improved, the process is simplified, the cost can be reduced, and the application prospect is better.

This invention belongs to the field of molecular biology technology, specifically relating to a method and application for detecting target substances in trace samples. The detection method includes the following steps: S100: acquiring quantitative results of target substance detection in a signal correction sample, a signal reference sample, and a mixed sample; S200: correcting the quantitative results of the signal reference sample and the mixed sample using the quantitative results of the signal correction sample, obtaining corrected results for the signal reference sample and the mixed sample; S300: comparing the corrected results of the mixed sample with the corrected results of the signal reference sample to obtain the quantitative result of the target substance in the trace sample. This invention can significantly improve detection sensitivity. It reduces the dependence on ultra-high sensitivity mass spectrometers, enabling conventional mass spectrometry instruments to detect trace target substances. By setting up a signal enhancement sample, the signal of the trace target substance is made higher than the noise signal, and the peak shape is improved, thus enhancing the stability of detection in trace samples.