Wireless test strip analyzer and use method thereof

Abstract

The invention discloses a wireless test strip analyzer and a use method thereof, and relates to the technical field of biological detection. The analyzer comprises a circuit board, a test strip clamping groove, a battery and a protective shell, the circuit board, the test strip clamping groove and the battery are arranged in the protective shell, and the battery is connected with the circuit board; the test strip clamping groove is used for placing a test strip; an amplification product based on a bionic dandelion isothermal amplification system is dropwise added on the test strip and is used for detecting the number of CTCs. According to the invention, the sensitivity of CTCs detection can be improved, the detection cost is reduced, and the dependence on expensive instruments is reduced.

Description

Technical Field

[0001] The present invention relates to the field of biological detection technology, and in particular to a wireless test strip analyzer and a method for using the same. Background Art

[0002] The detection of circulating tumor cells (CTCs) is crucial, but faces challenges such as low sensitivity, high cost, and complex procedures. Current CTC detection methods lack sufficient sensitivity, and high-precision CTC detection relies on expensive advanced instruments such as single-cell sequencing platforms and microfluidic chip devices. These high costs and complex instruments hinder widespread application. Summary of the Invention

[0003] The object of the present invention is to provide a wireless test strip analyzer and a method of using the same, aiming to solve or improve at least one of the above-mentioned technical problems.

[0004] To achieve the above object, the present invention provides the following solutions:

[0005] A wireless test strip analyzer comprises: a circuit board, a test strip slot, a battery, and a protective shell; the circuit board, the test strip slot, and the battery are built into the protective shell, and the battery is connected to the circuit board; the test strip slot is used to accommodate a test strip; an amplification product based on a bionic dandelion isothermal amplification system is dripped onto the test strip for detecting the number of CTCs.

[0006] Optionally, the circuit board integrates a 365nm ultraviolet LED, an XYZ true color sensor, a Bluetooth 5.1 system-on-chip, and a 3.3V power supply.

[0007] Optionally, the Bluetooth 5.1 system-level chip is also wirelessly connected to the user's smartphone to receive wireless instructions sent by the user's smartphone and transmit detection information.

[0008] Optionally, the bionic dandelion-based isothermal amplification system consists of a hexapod DNA walker, nonlinear DNA self-assembly technology and an asymmetric carrier with high signal probe loading efficiency; the asymmetric carrier with high signal probe loading efficiency uses AuFe Janus nanoparticles.

[0009] The present invention also provides a method for using the wireless test strip analyzer, using the analyzer as described above, comprising:

[0010] Power on and initialization: After the analyzer is powered on, a Bluetooth connection is established with the smartphone via the BLE MCU, and the smartphone wirelessly sends control commands to the BLE MCU.

[0011] UV LED activation: After receiving the command, the BLE MCU sets a specific GPIO pin to high, activating the NMOS transistor to turn on, thereby lighting up the UV LED;

[0012] XYZ True Color Sensor Communication: The BLE MCU establishes communication with the XYZ True Color Sensor via the I2C protocol to complete sensor parameter configuration and data acquisition. The XYZ True Color Sensor converts captured light signals into XYZ tristimulus values ​​based on the International Commission on Illumination 1931 color space standard, enabling standardized measurement of fluorescence intensity. The XYZ True Color Sensor also has a built-in signal processing unit that transmits the processed digital light signals directly to the BLE MCU via the I2C interface.

[0013] Data transmission and power consumption management: After completing the color data measurement, the BLE MCU controls the sensor to enter power-down mode and turns off the UV LED to reduce power consumption;

[0014] Result transmission: The BLE MCU eventually transmits the collected XYZ data to the smartphone via Bluetooth. The user then evaluates the fluorescence intensity of the T-line of the test strip based on the received XYZ values.

[0015] According to the specific embodiments provided by the present invention, the present invention discloses the following technical effects:

[0016] The present invention discloses a wireless test strip analyzer and its use method. The analyzer includes a circuit board, a test strip slot, a battery, and a protective case. The circuit board, the test strip slot, and the battery are built into the protective case, and the battery is connected to the circuit board. The test strip slot is used to hold a test strip. The test strip is dripped with an amplification product based on a biomimetic dandelion isothermal amplification system for detecting the number of CTCs. The present invention can improve the sensitivity of CTC detection, reduce detection costs, and reduce reliance on expensive instruments. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0018] Figure 1 The figure is a schematic structural diagram of the wireless test strip analyzer of the present invention.

[0019] Figure 2 The figure is a schematic diagram of the circuit design of the wireless test strip analyzer of the present invention.

[0020] Figure 3Schematic diagram of the effects of different concentrations of MCF-7 cells in this example; wherein, part (a) is a physical image of the corresponding fluorescent test strips for detection of different concentrations of MCF-7 cells (0, 10, 100, and 1000 cells / mL); part (b) is a T-line fluorescence quantitative histogram of the test strips for different concentrations of MCF-7 cells detected by the wireless test strip analyzer of the present invention.

[0021] Figure 4 Detailed circuit diagram of the wireless test strip analyzer in this embodiment. DETAILED DESCRIPTION

[0022] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0023] The object of the present invention is to provide a wireless test strip analyzer and a method of using the same, aiming to solve or improve at least one of the above-mentioned technical problems.

[0024] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0025] like Figures 1-4 As shown, the present invention provides a wireless test strip analyzer, comprising: a circuit board, a test strip slot, a battery and a protective shell; the circuit board, the test strip slot and the battery are built into the protective shell, and the battery is connected to the circuit board; the test strip slot is used to place a test strip; the test strip is dripped with an amplification product based on the bionic dandelion isothermal amplification system for detecting the number of CTCs.

[0026] The bionic dandelion isothermal amplification system, which consists of a hexapod DNA walker, nonlinear DNA self-assembly technology, and asymmetric AuFe Janus nanoparticles with high signal probe loading efficiency, demonstrated remarkable amplification efficiency. Compared with traditional isothermal amplification systems, the bionic dandelion isothermal amplification system has an efficiency increase of approximately 6.72 times.

[0027] As a specific embodiment, the detection system mainly consists of a biomimetic dandelion isothermal amplification system, a lateral flow immunochromatographic test strip, and a wireless test strip analyzer. First, under the action of a magnetic field, anti-EpCAM monoclonal antibodies labeled with magnetic beads are used to enrich and separate CTCs from red blood cell lysed blood. Then, the biomimetic dandelion isothermal amplification system is added. CTCs can competitively bind to the aptamer, thereby releasing the aptamer-enclosed hexapod DNA walker. In Mg 2+ With the assistance of a DNAzyme, the hexapod DNAwalker acts as a DNAzyme, specifically cleaving the RNA bases of D-RNA and efficiently releasing dandelion seed-like DNA macromolecules. This process is very similar to the natural phenomenon of dandelion hairs flying off, so the system was named the bionic dandelion isothermal amplification system. As the amplification product of the bionic dandelion isothermal amplification system, the bionic dandelion seeds are magnetically separated and then dripped onto the sample pad of the test strip. After 15 minutes, the fluorescence intensity is measured, and by establishing a positive correlation between the number of CTCs and the fluorescence intensity, the number of CTCs can be accurately detected.

[0028] As a more specific implementation method, construct Figure 1 The wireless test strip analyzer shown in the figure is mainly composed of four parts: a highly integrated circuit board, a test strip card slot, a battery and a protective shell ( Figure 1 The board integrates a 365nm UV LED, an XYZ true color sensor, a Bluetooth 5.1 system-on-chip, and a 3.3V power supply ( Figure 1 The user controls the fluorescence detector via wireless commands sent from a smartphone. Upon receiving the commands, the Bluetooth chip system activates the UV LED and fluorescence sensor to measure the fluorescence intensity of the T-line on the test strip. The sensor then captures the fluorescence information and outputs it as X, Y, and Z values.

[0029] The wireless LFIA test strip analyzer uses an XYZ true color sensor to determine the concentration of CTCs by analyzing the fluorescence intensity of the T line on the LFIA test strip under ultraviolet light. Its specific working mechanism is as follows:

[0030] 1. Bluetooth connection and command transmission

[0031] After powering on, the analyzer establishes a Bluetooth connection with the smartphone via its Bluetooth Low Energy microcontroller unit (BLE MCU, model HJ-531IMH). The smartphone then wirelessly sends control commands to the MCU.

[0032] 2. UV light source activation

[0033] When the BLE MCU receives the command, it sets the general-purpose input and output (GPIO) pin to a high level, triggering the N-channel metal oxide semiconductor field effect transistor (MOSFET), thereby turning on the UV LED.

[0034] 3. Sensor communication and data acquisition

[0035] Subsequently, the BLE MCU establishes communication with the XYZ true color sensor (model AS73211) through the I2C protocol to complete sensor parameter configuration and data collection.

[0036] The sensor's core function is to convert captured light signals into hexadecimal values ​​based on the International Commission on Illumination's 1931 color space standard, enabling precise quantification of fluorescence intensity. Its integrated signal processing unit transmits the processed digital light signals directly to a Bluetooth Low Energy (BLE) MCU via an I2C interface.

[0037] 4. Low power mode activated

[0038] After data collection is completed, the BLE MCU will instruct the color sensor to enter the power-down state and turn off the UV LED to reduce power consumption.

[0039] 5. Wireless data transmission and result analysis

[0040] Finally, the collected hexadecimal values ​​are wirelessly transmitted to a smartphone via Bluetooth, allowing users to correlate fluorescence intensity with CTCs concentration.

[0041] Through this design, the analyzer uses advanced components such as the XYZ true color sensor and optimized signal processing technology to achieve accurate and efficient fluorescence detection, ensuring the accuracy of result interpretation.

[0042] Subsequently, the feasibility of the wireless cell sensor based on the wireless LFIA test strip analyzer was evaluated. The feasibility of the wireless cell sensor was verified by detecting MCF-7 cells at concentrations of 0, 10, 100, and 1000 cells. Figure 3 ). The verification results show that the fluorescence intensity of the T-line of the test strip is proportional to the increase in cell concentration ( Figure 3 (a)). However, no obvious fluorescent band was observed in the T-line of the blank sample. The test strip was then inserted into the wireless test strip analyzer for more accurate quantification ( Figure 3 (b) The results obtained from the wireless test strip analyzer not only agreed with the visual interpretation of the test strip but also provided precise values. These findings demonstrate the feasibility of the wireless test strip analyzer and its ability to ensure accurate detection of CTCs.

[0043] in conclusion

[0044] In this example, a novel wireless cell sensor was successfully developed, combining a biomimetic dandelion isothermal system, a test strip platform, and a wireless test strip analyzer. Based on biomimetic principles, the biomimetic dandelion isothermal system was designed to improve amplification efficiency. Due to the synergistic effect of the high CuInS2@ZnS quantum dot loading rate of the biomimetic dandelion seeds, the fluorescent signal probe loading rate of the AuFe JNPs, and the high enzymatic cleavage efficiency of the hexapod DNA walker, the amplification efficiency of the biomimetic dandelion isothermal system was 6.72 times higher than that of traditional isothermal amplification systems. The use of the test strip platform also facilitates operation. The cell sensor demonstrated excellent performance in terms of sensitivity, specificity, and reproducibility. Its ease of operation, in particular, makes this wireless cell sensor advantageous for primary healthcare and large-scale screening. Notably, clinical sample trials demonstrated that this method has high accuracy and anti-interference capabilities, making it suitable for the detection of CTCs in whole blood samples. Therefore, the newly developed wireless cell sensor has great potential for clinical detection of CTCs.

[0045] As another specific implementation, a method for using the wireless test strip analyzer is described in detail.

[0046] The wireless test strip analyzer uses an XYZ true color sensor to determine the concentration of CTCs by analyzing the fluorescence intensity of the T-line on the test strip under ultraviolet light. The specific operation steps are as follows:

[0047] 1. Power on and initialization: After the analyzer is powered on, it establishes a Bluetooth connection with the smartphone via the BLE MCU (model HJ-531IMH). The smartphone wirelessly sends control commands to the BLE MCU.

[0048] 2. UV LED activation: After receiving the command, the BLE MCU sets a specific GPIO pin to high, activating the NMOS transistor to turn on, thereby lighting up the UV LED.

[0049] 3. XYZ True Color Sensor Communication: The BLE MCU establishes communication with the XYZ True Color Sensor (model AS73211) via the I2C protocol to complete sensor parameter configuration and data acquisition. Based on the International Commission on Illumination (CIE) 1931 color space standard, the sensor converts captured light signals into XYZ values. This set of values ​​(X, Y, Z) accurately represents the human eye's perception of color and enables standardized measurement of fluorescence intensity. The sensor's built-in signal processing unit transmits the processed digital light signal directly to the BLE MCU via the I2C interface.

[0050] 4. Data transmission and power consumption management: After completing the color data measurement, the BLE MCU controls the sensor to enter power-down mode and turns off the UV LED to reduce power consumption.

[0051] 5. Result transmission: The BLE MCU ultimately transmits the collected XYZ data to the smartphone via Bluetooth. The user then evaluates the fluorescence intensity of the T-line on the test strip based on the received XYZ values.

[0052] The design uses advanced components such as XYZ true color sensors, combined with optimized signal processing algorithms, to ensure the accuracy and efficiency of fluorescence detection, providing a reliable basis for test strip judgment.

[0053] Among them, the wireless test strip analyzer uses a 365nm ultraviolet LED to excite the fluorescent substance on the test paper, and analyzes the effective information expressed by the test paper by detecting the fluorescence intensity. The user controls the operation of the fluorescence detector through wireless instructions sent by the smartphone. After receiving the instruction, the Bluetooth 5.1 system-level chip activates the ultraviolet LED and the XYZ true color sensor to measure the fluorescence on the test paper. Specifically, after the ultraviolet light excites the fluorescent substance, the sensor captures the color information of the fluorescent reflection and outputs it in the form of X, Y, and Z values ​​in the XYZ color space. The sensor transmits the data to the Bluetooth 5.1 system-level chip through the I2C interface, which processes the data and then transmits the test results wirelessly to the smartphone via Bluetooth. The ultraviolet LED is directly powered by the battery to provide sufficient driving current to ensure that the optical power of the ultraviolet light can effectively excite the fluorescent substance. At the same time, the XYZ true color sensor and the Bluetooth 5.1 system-level chip are powered by a 3.3V power supply regulated by a low-dropout regulator (LDO) to ensure their normal operation. The circuit diagram of the wireless test strip analyzer is shown in the figure below. Figure 2 The corresponding detailed circuit schematic is shown in Figure 4 The wireless test strip analyzer realizes a complete functional chain of fluorescence excitation, color detection and wireless data transmission, and is suitable for portable fluorescence detection application scenarios.

[0054] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0055] This document uses specific examples to illustrate the principles and implementation methods of the present invention. The above examples are only intended to help understand the core concept of the present invention. At the same time, those skilled in the art will find that the specific implementation methods and application scopes may vary based on the concept of the present invention. In summary, the contents of this specification should not be construed as limiting the present invention.

Claims

1. A wireless test strip analyzer, characterized in that: include: A circuit board, a test strip slot, a battery and a protective shell; the circuit board, the test strip slot and the battery are built into the protective shell, and the battery is connected to the circuit board; the test strip slot is used to place a test strip; the test strip is dripped with an amplification product based on the bionic dandelion isothermal amplification system for detecting the number of CTCs.

2. The wireless test strip analyzer according to claim 1, characterized in that: The circuit board integrates a 365nm UV LED, an XYZ true color sensor, a Bluetooth 5.1 system-on-chip, and a 3.3V power supply.

3. The wireless test strip analyzer according to claim 2, characterized in that: The Bluetooth 5.1 system-level chip is also wirelessly connected to the user's smartphone to receive wireless commands sent by the user's smartphone and transmit detection information.

4. The wireless test strip analyzer according to claim 1, characterized in that: The bionic dandelion-based isothermal amplification system consists of a hexapod DNA walker, nonlinear DNA self-assembly technology, and an asymmetric carrier with high signal probe loading efficiency; the asymmetric carrier with high signal probe loading efficiency uses AuFe Janus nanoparticles.

5. A method for using a wireless test strip analyzer, using the analyzer according to any one of claims 1 to 4, characterized in that: include: Power on and initialization: After the analyzer is powered on, a Bluetooth connection is established with the smartphone via the BLE MCU, and the smartphone wirelessly sends control commands to the BLE MCU. UV LED activation: After receiving the command, the BLE MCU sets a specific GPIO pin to high, activating the NMOS transistor to turn on, thereby lighting up the UV LED; XYZ True Color Sensor Communication: The BLE MCU establishes communication with the XYZ True Color Sensor via the I2C protocol to complete sensor parameter configuration and data acquisition. The XYZ True Color Sensor converts captured light signals into XYZ tristimulus values ​​based on the International Commission on Illumination 1931 color space standard, enabling standardized measurement of fluorescence intensity. The XYZ True Color Sensor also has a built-in signal processing unit that transmits the processed digital light signals directly to the BLE MCU via the I2C interface. Data transmission and power consumption management: After completing the color data measurement, the BLE MCU controls the sensor to enter power-down mode and turns off the UV LED to reduce power consumption; Result transmission: The BLE MCU eventually transmits the collected XYZ data to the smartphone via Bluetooth. The user then evaluates the fluorescence intensity of the T-line of the test strip based on the received XYZ values.

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