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449 results about "Hybridization reaction" patented technology
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Analying polynucleotide sequences
InactiveUS6054270AStable duplexReduce impactSequential/parallel process reactionsSugar derivativesHybridization reactionSequence determination
This invention provides an apparatus and method for analyzing a polynucleotide sequence; either an unknown sequence or a known sequence. A support, e.g. a glass plate, carries an array of the whole or a chosen part of a complete set of oligonucleotides which are capable of taking part in hybridization reactions. The array may comprise one or more pair of oligonucleotides of chosen lengths. The polynucleotide sequence, or fragments thereof, are labelled and applied to the array under hybridizing conditions. Applications include analyses of known point mutations, genomic fingerprinting, linkage analysis, characterization of mRNAs, mRNA populations, and sequence determination.
Owner:OXFORD GENE TECH
Encoded solid supports for biological processing and assays using same
InactiveUS20090226891A2Sequential/parallel process reactionsNon-electrical signal transmission systemsAntifuseSolid phases
Owner:IRORI TECH
Microarray hybridization device having bubble-fracturing elements
InactiveUS7238521B2Degree of improvementImprove uniformityBioreactor/fermenter combinationsRotating receptacle mixersMicrobubblesHybridization reaction
A novel hybridization device that improves the efficiency and consistency of microarray hybridization reactions by achieving a greater degree of internal mixing of target solution. The device provides a gasket-and-cover-type chamber wherein solution mixing is achieved by the creation of a multitude of microbubbles. One or more of the inner walls that define the chamber contain bubble-rupturing elements that extend into the chamber and terminate in sharp edges. They are typically located on opposite sides of a rectangular chamber and are pointed in a direction opposing bubble movement. Their interference with larger bubbles causes their breakup into microbubbles which travel separate and distinct paths as a result of external agitation and thereby provide improved solution mixing that results in a uniform distribution of target molecules to the probe molecules bound to the substrate. The sensitivity and consistency of the hybridization reaction is significantly increased.
Owner:BIOCEPT INC
Apparatus for substrate handling
InactiveUS20050042768A1Preserve integrityDry substrate surfaceMaterial nanotechnologyPeptide librariesChemical reactionControl manner
Devices, apparatus and methods are disclosed for carrying out processing steps involved in chemical reactions such as hybridization reactions conducted on the surface of a substrate comprising chemical compounds such as biopolymer features. A device comprises a housing comprising a housing chamber. The housing comprises an opening through which a substrate may be inserted into the housing chamber. The device may also comprise a lift mechanism for moving the substrate in and out of the housing chamber in a controlled manner. At least one inlet may be in fluid communication with the housing chamber and at least one outlet may be in fluid communication with the housing chamber. Also disclosed are methods wherein the surface is brought into contact with a processing fluid; and, then, the surface is removed from contact with the fluid in a controlled manner at a rate that substantially eliminates droplet formation of the fluid on the surface of the substrate.
Owner:AGILENT TECH INC
Modified polynucleotides and uses thereof
InactiveUS6867290B2Improve stabilityReduce potential blockSugar derivativesMicrobiological testing/measurementOligoribonucleotidesPolyribonucleotides
Owner:CYCLOPS GENOME SCI
Method for carbon fiber surface modification of plasma coated with nano colloidal sols by plasma treatment
InactiveCN101413209AImprove performanceImprove surface propertiesPhysical treatmentCarbon fibresFiberCarbon fibers
The invention relates to a method for modifying the surface of carbon fiber coated with nano sol through plasma treatment, which comprises the following steps: firstly, nano particles are prepared into an organic solvent, a sol solution of water or a sol solution prepared by hybridization reaction of a precursor solution of organic-inorganic nano particles by the ultrasonic vibrating technology; secondly, the sol solution is coated on the surface of the carbon fiber, treated by means of spray coating and padding, and dried; and thirdly, the dried carbon fiber is placed on a special transport unit for plasma processing equipment and a plasma is sprayed on the surface of the carbon fiber to make the carbon fiber move in the plasma atmosphere, so as to generate surface modification, wherein the treating power is between 10 and , watts, and the treatment time is between 0.5 and 300 seconds. The method can effectively improve the performance of the fiber, improves the molded manufacturability and the overall properties of composite materials of the fiber, has simple technology, quick processing speed, good treatment effect and low cost, is convenient to operate and difficult to cause environmental pollution, can reduce energy consumption, and is suitable for industrial production.
Owner:DONGHUA UNIV +1
Microarray hybridization device
InactiveUS20050112589A1Increase the degree of mixingImprove uniformityRotating receptacle mixersBioreactor/fermenter combinationsMicrobubblesHybridization reaction
A novel hybridization device that improves the efficiency and consistency of microarray hybridization reactions by achieving a greater degree of internal mixing of target solution. The device provides a gasket-and-cover-type chamber wherein solution mixing is achieved by the creation of a multitude of microbubbles. One or more of the inner walls that define the chamber contain bubble-rupturing elements that extend into the chamber and terminate in sharp edges. They are typically located on opposite sides of a rectangular chamber and are pointed in a direction opposing bubble movement. Their interference with larger bubbles causes their breakup into microbubbles which travel separate and distinct paths as a result of external agitation and thereby provide improved solution mixing that results in a uniform distribution of target molecules to the probe molecules bound to the substrate. The sensitivity and consistency of the hybridization reaction is significantly increased.
Owner:BIOCEPT INC
Multiplex polynucleotide synthesis
InactiveUS20060234264A1Solve low usageMicrobiological testing/measurementFermentationChemical treatmentDna polymerasen
The invention provides a method of synthesizing complex mixtures of long polynucleotides by separately synthesizing and assembling shorter component oligonucleotides. In one aspect, pairs of oligonucleotides that form components of such polynucleotides are synthesized on one or more microarrays, or other large-scale parallel solid phase synthesis platforms, after which they are released. Members of each pair contain unique complementary barcode sequences that are used match-up pairs in a hybridization reaction to form duplexes. Such duplexes are then extended with a DNA polymerase and the resulting extension product is amplified to form an amplicon. The amplicon may be either used directly as the desired polynucleotide, or it may undergo further processing, such as capture on solid phase supports and / or additional enzymatic or chemical processing, to produce a desired polynucleotide product, such as a circularizing probe for multiplex analysis of genomic DNA, or the like.
Owner:AFFYMETRIX INC
Rolling circle amplification-based colorimetric assay method for target nucleic acids or proteins
ActiveCN102586450AReduce usageEasy to operateMicrobiological testing/measurementBiological testingMicroorganismMagnetic bead
The invention relates to a rolling circle amplification-based colorimetric assay method for target nucleic acids or proteins. The method mainly utilizes the hybridization between nucleic acids and antigen-antibody binding to indirectly fix the target nucleic acids or proteins to be assayed onto magnetic beads, rolling circles are added, a rolling circle primer on hybrid is utilized to carry out constant-temperature rolling circle amplification, and finally, the chromogenic reaction of nanogold is utilized to assay constant-temperature amplified macromolecular product in order to achieve the purpose of relatively assaying the target nucleic acids and proteins. By integrating rolling circle amplification and the chromogenic reaction of nanogold, the method not only amplifies a large quantity of micro-sample signals, but also realizes the rapid, visual assay of results, avoids the usage of a PCR (Polymerase Chain Reaction) instrument and other expensive instruments, and decreases the requirement on the experimental hardware condition. The method is expected to be applied in bedside rapid diagnosis and the field rapid assay of microorganisms in food safety and the environment.
Owner:SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
Information processing apparatus, information processing method, storage medium and program
InactiveUS20050164217A1Determine biological species easily, inexpensivelyShort timeMicrobiological testing/measurementBiological testingInformation processingHybridization reaction
Biological species are determined easily, inexpensively, in a short time, and accurately even if a plurality biological species having base sequences similar to one another exist in a specimen. For achieving such an object, the information processing apparatus according to the present invention is an information processing apparatus processing information about the signal intensity of each probe obtained as a result of making a predetermined specimen undergo a hybridization reaction using a DNA micro-array in which probes being nucleic acid complementary to some of nucleotide sequences of biological species, the information processing apparatus comprising unit configured to retain a known sample, unit configured to acquire an unknown sample obtained as a result of making the predetermined specimen undergo the hybridization reaction, unit configured extract a vector related to a vector related to a predetermined biological species, of the known sample and unknown sample, and determining unit configured to compare the extracted vector of the known sample with the vector of the unknown sample to determine whether or not the predetermined biological species is contained in the predetermined specimen.
Owner:CANON KK
Method for carbon fiber surface modification of plasma coated with silicon dioxide by plasma treatment
InactiveCN101413210AImprove surface propertiesImprove performancePhysical treatmentCarbon fibresFiberCarbon fibers
The invention relates to a method for modifying the surface of carbon fiber coated with nano dioxide sol through plasma treatment, which comprises the following steps: firstly, nano silicon dioxide is prepared into an organic solvent, a sol solution of water or a sol solution prepared by hybridization reaction of a precursor solution of organic-inorganic nano particles and silicon dioxide nano particles by the ultrasonic vibrating technology; secondly, the sol solution is coated on the surface of the carbon fiber, treated by means of spray coating or padding and so on, and dried; and thirdly, the carbon fiber is placed on a plasma transport unit and a plasma is sprayed on the surface of the carbon fiber to generate modification, wherein the treating power is between 10 and 15,000 watts, and the treatment time is between 0.5 and 300 seconds. The method can effectively improve the performance of the carbon fiber, improves the molded manufacturability and the overall properties of composite materials of the carbon fiber, has simple technology, quick processing speed, good treatment effect and low cost, is convenient to operate and difficult to cause environmental pollution, can reduce energy consumption, and is suitable for industrial production.
Owner:DONGHUA UNIV +1
Method of detecting adenosine with fluorescent sensor on the basis of aptamer
InactiveCN106950206AEffective quenchingHigh absorption coefficientFluorescence/phosphorescenceEnergy transferAnalysis data
The invention discloses a method of detecting adenosine with a fluorescent sensor on the basis of an aptamer. In the method, gold nano particles modified with the aptamer are used as a recognition probe and an energy acceptor, and carbon dots modified with an aptamer complementary chain are used as a fluorescent probe and an energy donor; through a hybridization reaction, fluorescence resonance energy transfer is carried out between the gold nano particles and the carbon dots, and fluorescence of the detection system is quenched. After addition of adenosine, the adenosine and the aptamer complementary chain are competitively combined with the aptamer, so that energy transfer efficiency between the gold nano particles and the carbon dots is weakened; and the fluorescence of the detection system is recovered, so that based on the change of the fluorescence signal, the method achieves quantitative detection of the adenosine. The method has strong specificity and simple operation, is low in required quantity of a sample, is high in sensitivity, can be used for detecting the adenosine in a blood sample and supplies useful analysis data for clinical diagnosis on diseases.
Owner:NANJING MEDICAL UNIV
DNA electrochemical sensor and preparation method thereof
InactiveCN101078026AHigh densityStrong Mismatch CapabilityMicrobiological testing/measurementHybridization reactionElectrochemistry
The invention describes a kind of DNA electrochemistry sensor, which comprises gold pole, complementary DNA, gold nano-particles and electrochemistry indicator. It is characterized in that one segment of complementary DNA is assembled onto the gold pole as capture probe, and the other segment as detection probe is integrated with the gold nano-particles to be the carrier of electrochemistry indicator. The preparation method is disclosed. By means of 'sandwich' hybridization method, the surface of the pole has high DNA chains with negative charge and The VIII family transition metal ions with positive charge was introduced to combine with DNA as electrochemistry indicator. The DNA hybridization reaction is indicated by detecting the electrochemistry numerical change (electric current or quantity) of the pole surface. Because of the amplification effect of the gold nano-particles, the sensor can detect trace DNA with high selectivity and the gold pole modified with DNA can be used repeatedly.
Owner:JIANGSU WUZHONG HIGH & NEW TECH IND
Heat radiation material, heat radiation structure, and preparation method thereof
InactiveCN103205149AGood compatibilityImprove stabilityLayered productsFilm/foil adhesivesDispersion stabilitySilicon oxygen
The invention provides a heat radiation material, a heat radiation structure, and preparation methods thereof. The heat radiation material comprises, by weight, 10-30 parts of inorganic heat radiation nano-grade material aqueous slurry, 40-80 parts of aqueous high-molecular resin, 0.5-5 parts of an auxiliary agent, and 5-20 parts of a diluting agent. The inorganic heat radiation nano-grade material aqueous slurry comprises, by weight, 10-25 parts of an inorganic heat radiation nano-grade material, 0.5-20 parts of a bi-functional large-molecular modifier, and 50-100 parts of a solvent. According to the inorganic heat radiation nano-grade material, the bi-functional large-molecular modifier is used in surface modification. Selective absorption and grafting hybridization reaction are carried out on the surface of the inorganic heat radiation nano-grade material, such that coordination self-assembly behaviors of ester bond, silicon-oxygen bond, hydrogen bond, and the like are formed on the surface of the material. Therefore, inorganic heat radiation nano-grade material interface performance is controlled, compatibility and system dispersion stability of the inorganic heat radiation nano-grade material are improved, and better heat radiation performance can be obtained.
Owner:REGAL PAPER TECH
Apparatus for substrate handling
InactiveUS7517498B2Preserve integrityDry surfaceMaterial nanotechnologyPeptide librariesChemical reactionControl manner
Devices, apparatus and methods are disclosed for carrying out processing steps involved in chemical reactions such as hybridization reactions conducted on the surface of a substrate comprising chemical compounds such as biopolymer features. A device comprises a housing comprising a housing chamber. The housing comprises an opening through which a substrate may be inserted into the housing chamber. The device may also comprise a lift mechanism for moving the substrate in and out of the housing chamber in a controlled manner. At least one inlet may be in fluid communication with the housing chamber and at least one outlet may be in fluid communication with the housing chamber. Also disclosed are methods wherein the surface is brought into contact with a processing fluid; and, then, the surface is removed from contact with the fluid in a controlled manner at a rate that substantially eliminates droplet formation of the fluid on the surface of the substrate.
Owner:AGILENT TECH INC
Method for detecting miRNA (ribonucleic acid)
InactiveCN103698375AHigh sensitivityStrong specificityMicrobiological testing/measurementMaterial electrochemical variablesHybridization reactionCatalytic oxidation
The invention discloses a method for detecting miRNA (ribonucleic acid). The method comprises the steps: (1) synthesizing a DNA (deoxyribonucleic acid) tetrahedron probe; (2) connecting three peak points of the DNA tetrahedron probe to the surface of a working electrode of an electrochemical device to obtain a working electrode with a capture probe; (3) adding a target miRNAs to be detected, a signal probe and an auxiliary chain into a reaction system to implement hybridization reaction to form a composite body I, and immersing the working electrode into the reaction system to implement hybridization reaction to form a composite body II; (4) generating reaction between the composite body II and enzyme capable of generating catalytic oxidation reduction reaction; (5) adding a primer produced by the enzyme catalytic oxidation to realize electrochemical detection analysis. The target miRNAs can be directly detected by the method disclosed by the invention without being marked and subjected to pre-PCR (polymerase chain reaction) multiplication; the method is easy to operate, so that the experiment cost is greatly reduced, and the experiment efficiency is improved.
Owner:SHANGHAI INST OF MEASUREMENT & TESTING TECH
Method for modifying ultrahigh molecular weight polyethylene fiber by plasma treatment
InactiveCN101532244AIncrease infiltration speedImprove wettabilityPhysical treatmentFibre typesFiberPlasma generator
The invention relates to a method for modifying ultrahigh molecular weight polyethylene fiber by plasma treatment. The method comprises that: (1) a nanometer particle of silicon dioxide is prepared into an organic solvent or water solution of sol of which the mass percent is 0.01 to 15 percent, by an ultrasonic shock technique; or (2) a precursor solution of the organic-inorganic nanometer particle is subjected to hybridization reaction with the nanometer particle of silicon dioxide to prepare the nanometer silicon dioxide sol solution; (3) the sol solution is coated on the surface of the ultrahigh molecular weight polyethylene fiber by spraying or padding; and (4) the ultrahigh molecular weight polyethylene fiber is dried at a temperature of 10 to 150 DEG C, and the solvent is collected; and the ultrahigh molecular weight polyethylene fiber is introduced in a plasma atmosphere region by a plasma generator for plasma surface modification. The compound property between the treated ultrahigh molecular weight polyethylene fiber and the organic matrix materials is greatly improved; moreover, the method also has the advantages of simple process, good treatment effect, low cost, environmental protection and low energy consumption.
Owner:DONGHUA UNIV +1
Method for modifying nanometer sol ultrahigh molecular weight polyethylene fiber by plasma treatment
InactiveCN101532239AImprove permeabilityHigh chemical activityPhysical treatmentFibre typesFiberTherapeutic effect
The invention relates to a method for modifying a nanometer sol ultrahigh molecular weight polyethylene fiber by plasma treatment. The method comprises that: (1) inorganic nanometer particle is prepared into a sol solution by an ultrasonic shock technique; or (2) a precursor solution of the organic-inorganic nanometer particle is subjected to hybridization reaction with the inorganic nanometer particle to prepare the sol solution; (3) the sol solution is coated on the surface of the ultrahigh molecular weight polyethylene fiber; and (4) the ultrahigh molecular weight polyethylene fiber is dried at a temperature of 10 and 150 DEG C, and the solvent is collected; the ultrahigh molecular weight polyethylene fiber is introduced in a plasma atmosphere region by a plasma generator for plasma surface modification; and the treated ultrahigh molecular weight polyethylene fiber is on-line rolled by an automatic rolling machine. The compound property between the treated ultrahigh molecular weight polyethylene fiber and the organic matrix materials is greatly improved; moreover, the method also has the advantages of simple process, good treatment effect, low cost, environmental protection and low energy consumption.
Owner:DONGHUA UNIV +1
Preparation and application method of electrochemical reduction graphene oxide and nanogold modified electrode based DNA sensor
InactiveCN105758918AEasy to makeLow priceMaterial electrochemical variablesNanoparticleCarbon paste electrode
The invention discloses a novel electrochemical DNA sensor constructed by a nanogold and partially-reduced graphene oxide (p-RGO) modified ionic liquid carbon paste electrode (CILE) as a platform prepared by an electrochemical method, and a method of applying the CILE to detect the Listeria feature gene sequence. 1-hexylpyridiniumhexafluorophosphate as a modifier is used for preparing the substrate electrode CILE, gold nanoparticles are deposited on the surface of the substrate electrode CILE and then the p-RGO is prepared by controlling electrochemical reduction conditions; an amino-modified probe ssDNA is fixed on the surface of the electrode by an amido bond covalent bonding method through carboxyl groups remained on the surface of p-RGO to constitute ssDNA / p-RGO / AuNPs / CILE; methylene blue (MB) as an indicator is used for detecting the hybridization reaction after a target sequence is hybridized, and a differential pulse voltammetry (DPV) is used for detecting the Listeria feature gene segment.
Owner:QINGDAO UNIV OF SCI & TECH
Preparation method for high-hardness waterborne polyurethane dispersion
ActiveCN104231209AImprove water resistanceImprove adhesionPolyurea/polyurethane coatingsPolymer sciencePtru catalyst
The invention discloses a preparation method for high-hardness waterborne polyurethane dispersion. An inorganic nano material and waterborne polyurethane are grafted through an in-situ polymerization method, so that the hybridization reaction of inorganic materials and the organic materials is realized and complementary advantages are realized. The preparation method comprises the following operation steps: adding polyisocyanate, macromolecule polyol, a catalyst, a chain extender and a silane coupling agent according to certain weight ratio in a flask for reacting to obtain a polyurethane prepolymer; after the reaction is ended, sequentially adding a neutralizer, inorganic nano material dispersion liquid and deionized water under the condition of low-temperature and high-speed stirring; finally, heating under the vacuum condition for removing the solvent to obtain the high-hardness waterborne polyurethane dispersion. Compared with the traditional inorganic and organic blending process, the preparation method has the characteristics that products prepared through the preparation method have the advantages of good stability, excellent water resistance, long-time aging resistance, excellent and high adhesion, high drying speed, high hardness, good fullness, higher light transmittance and the like, and are wide in application range.
Owner:江苏日出化工有限公司
Inorganic heat-insulation nano material aqueous slurry, building glass heat-insulation coating and preparation method
InactiveCN101708958AImprove insulation performanceImprove stabilityDispersion stabilitySilicon oxygen
The invention provides a coating for building glass heat insulation which can release negative ions and a preparation method; the coating comprises inorganic heat-insulation nano material, siloxane polymer, inorganic heat-insulation nano material aqueous slurry of solvent, negative ion powder material aqueous slurry, aqueous resin, assistant and diluent; the inorganic heat-insulation nano material adopts siloxane polymer with discrete and exquisite structure as a template, selective adsorption and grafting hybridization reaction are carried out on the surface of the inorganic heat-insulation nano material, silicon-oxygen bond and hydrogen bond coordination self-assembly action is formed on the surface of the heat-insulation nano material to control the interface performance of the heat-insulation nano material, improve the compatibility and the system dispersion stability among the heat-insulation nano materials and obtain special optical and heat-insulation performances; in addition, the synergistic effect among the negative ion material, the inorganic heat-insulation nano material and the aqueous resin can improve the heat-insulation effect and the capacity of releasing the negative ions, so as to realize multi-functionalization of the building glass heat-insulation coating.
Owner:RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
Protein chip based on labeling streptavidin-biotin technology
InactiveCN1335505ABiological reaction amplificationHigh sensitivityCarrier-bound/immobilised peptidesBiological testingBiotin-streptavidin complexAntigen
The present invention relates to protein chip based on labeling streptavidin-biotin technology. The hybridization reaction includes the following steps: dropping tested sample to sample applied chip and washing out redundant sample with elution liquid; blocking wtih blockage liquid, washing and air drying; dropping protein marked with biotin diluted by blockage liquid, washing and air drying; anddropping fluorescnet or enzyme labeled streptavidin diluted by blockage liquid, washing and air drying. The protein chip of the present invention is used in determining antigen and antibody, and detecting specific matter combined with protein. The improved method of the present invention has clear, stable and reliable test results and wide application range.
Owner:GENE TECH SHANGHAI COMPANY
Method for determining melamine (Me) by chemiluminescence
The invention belongs to the field of chemiluminescence sensors, and relates to a method for determining melamine (Me), which is implemented by using a target induced chain releasing technique and combining with a hybrid chain reaction and a chemiluminescence technique. When a target Me exists, the Me acts with aptamer DNA1 (deoxyribonucleic acid 1) in double-stranded DNA on the surface of a functionalized magnetic bead (FMB1) so as to release a complementary sequence DNA2 of the aptamer; and the released complementary sequence DNA2 acts with another functionalized magnetic bead (FMB2), and a chain hybridization reaction is performed in the presence of hairpin DNA (HRP-H1 and HRP-H2) for modifying HRP, so that the two kinds of modified DNA grow into long double-stranded DNA. Through magnetic separation, and by using the catalytic action of HRP on a luminol-H2O2 chemiluminescence system, chemiluminescence is produced, and then the determination of the Me is realized through determining the chemiluminescence intensity. The method is high in selectivity and detection sensitivity.
Owner:广州倍腾新材料有限公司
Full-automatic hybridization appliance
ActiveCN102533525APromote the "civilization"Promote "civilianization"Bioreactor/fermenter combinationsBiological substance pretreatmentsHybridization reactionEngineering
The invention discloses a full-automatic hybridization appliance, which comprises a hybridization platform, a machine frame and a back plate. The machine frame and the back plate are fixed on the hybridization platform which is provided with a reagent supply device and a reagent preheating device, wherein the reagent supply device is matched with the reagent preheating device. The machine frame is provided with a hybridization reaction box, a feeding device for feeding reagent in the reagent supply box into the reaction box, an X-axis synchronous belt mechanism for driving the feeding device to reciprocate along the X-axis direction, a Z-axis synchronous belt mechanism for driving the feeding device to reciprocate along the Z-axis direction and a Y-axis synchronous belt mechanism for driving the hybridization reaction box to reciprocate along the Y-axis direction. The full-automatic hybridization appliance can achieve full-automatic operation of steps of washing, hybridizing, enzyme incubation, developing and the like in the gene chip operation technology, is low in manual participation, can greatly reduce production cost and effectively pushes generalized and wide application of gene chips.
Owner:亚能生物技术(深圳)有限公司
Method for modifying Kevlar fiber by treating nano sol through plasma
InactiveCN101575798AImprove permeabilityIncrease infiltration speedPhysical treatmentFibre typesFiberOrganic base
The invention relates to a method for modifying Kevlar fiber by treating a nano sol through plasma, which comprises the following steps: (1) preparing inorganic nano particles into sol solution by ultrasonic oscillation technology; or (2) carrying out a hybridization reaction of precursor solution of organic-inorganic nano particles and the inorganic nano particles to prepare sol solution; (3) coating the sol solution on the surface of the Kevlar fiber; (4) drying the Kevlar fiber at a temperature of between 10 and 150 DEG C, collecting solvent, then introducing the Kevlar fiber in a plasma atmosphere area through a plasma generating device for surface modification treatment by the plasma; and finally, carrying out on-line winding of the treated Kevlar fiber in an automatic winding machine and adjusting the wiring speed of the Kevlar fiber by adjusting the speed range of a winding shaft. The combined performance between the Kevlar fiber treated by the method and the organic base materials is improved greatly; and the method has simple process, good treatment effect, low cost, difficult environmental pollution and reduction of the energy consumption.
Owner:DONGHUA UNIV +1
Nonenzymatic catalytic signal amplification for nucleic acid hybridization assays
ActiveUS7005265B1Bioreactor/fermenter combinationsBiological substance pretreatmentsHybridization reactionNucleic Acid Probes
Devices, methods, and kits for amplifying the signal from hybridization reactions between nucleic acid probes and their cognate targets are presented. The devices provide partially-duplexed, immobilized probe complexes, spatially separate from and separately addressable from immobilized docking strands. Cognate target acts catalytically to transfer probe from the site of probe complex immobilization to the site of immobilized docking strand, generating a detectable signal. The methods and kits of the present invention may be used to identify the presence of cognate target in a fluid sample.
Owner:FAN WENHONG +2
Nucleic acid automatic examining device
InactiveUS20070264655A1Reduction in futile standby timeReduce standby timeBioreactor/fermenter combinationsBiological substance pretreatmentsHybridization reactionReaction step
A nucleic acid automatic examining device implements a hybridization reaction step after implementing a nucleic acid amplification reaction step on a nucleic acid sample containing a target nucleic acid. The device includes a nucleic acid amplification unit, a hybridization reaction unit, a dispenser unit for moving the nucleic acid sample from the nucleic acid amplification unit to the hybridization reaction unit, and a detector for detecting presence or absence of a reaction cassette in the hybridization reaction unit. When the detector detects the absence of the reaction cassette, the device implements steps up to the nucleic acid amplification reaction step and the dispenser unit is stopped so that the device may not implement the subsequent hybridization reaction step.
Owner:CANON KK
Visualization method for rapidly detecting trace amount of uranyl ions in water environment
InactiveCN104964942ARealize visual quick detectionHigh sensitivityMaterial analysis by observing effect on chemical indicatorColor/spectral properties measurementsMagnetic beadHorse radish peroxidase
The invention belongs to the field of detecting a trace amount of ions in the water environment, and particularly relates to a visualization method for rapidly detecting a trace amount of uranyl ions in the water environment. The method mainly includes the steps that DNAzyme with the specific recognition function on UO2 <2+> is fixed to the surfaces of magnetic beads, and horse radish peroxidase is preassembled on the surface of nano-gold; then the magnetic beads are connected with the nano-gold through the cutting effect of the UO2<2+> on the DNAzyme and the hybridization reaction of DNA, after separation and collection are carried out through an external magnetic field, H2O2 oxidation tetramethyl benzidine is efficiently catalyzed through the horse radish peroxidase to enable a solution to be changed from the blank to the blue, and therefore sensitive and specific visualization rapid detection of the UO2<2+> ions is achieved. As the method has the advantages of being high in sensitivity, high in specificity, high in matrix interference resistance, simple, rapid, low in cost and the like, the method can be used for site rapid visualization detection of the trace amount of UO2<2+> ions in various water samples.
Owner:FUZHOU UNIV
Single-wall carbon nano tube-based ultrasensitive deoxyribonucleic acid (DNA) biosensor and preparation method and application thereof
ActiveCN102262122AExcellent capacitance performanceOptimized areaMaterial analysis by electric/magnetic meansCarbon nanotubeSingle strand
The invention provides a single-wall carbon nano tube-based ultrasensitive deoxyribonucleic acid (DNA) biosensor and a preparation method and application thereof. In the method, single-wall carbon nano tubes (SWCNTs) are grown on the surface of a silicon wafer on site by a chemical vapor deposition method, and gold nano particles are deposited on the surfaces of carbon nano tube electrodes by an electrochemical deposition technology. A single-stranded DNA (ssDNA) probe is self-assembled to the surfaces of SWCNTs-Au electrodes and subjected to hybridization reaction with complementary ssDNA. The change of electron transfer resistance before and after hybridization is recorded by utilizing the unique specific surface areas of the carbon nano tubes and the quick dynamic characteristics of the electrodes and by an electrochemical impedance method under the action of current signal amplification of nanometer gold to realize the quantitative detection of the complementary DNA. The detection limit of the sensor on the target DNA can reach between 10 and 20 M, so the sensor has the advantages of high sensitivity and selectivity, capability of being used repeatedly and the like, and has important significance in fields of medical diagnosis, the food industry, environment friendliness and the like.
Owner:WENZHOU UNIVERSITY
Liquid phase chip for detecting breast cancer prognosis-related gene mRNA expression level
ActiveCN101921858AStrong specificityImprove signal-to-noise ratioMicrobiological testing/measurementSignal-to-noise ratio (imaging)Microsphere
The invention discloses a liquid phase chip for detecting breast cancer prognosis-related gene mRNA expression level, which mainly comprises a microsphere which aims at different target genes and is coupled to amido modified supporting probes, supporting extended probes connecting the supporting probes and target gene mRNA and amplification extended probes. Each supporting probe mainly comprises a 5' end spacer arm sequence and a 3' end specific sequence P1 which is in complementary pairing with a supporting extended probe; the supporting probe comprises SEQ. ID NO.1 specific to ESR1gene and SEQ. ID NO.2 specific to PGR gene; each supporting extended probe mainly comprises a 5' end specific sequence P2 which can be correspondingly combined with the target genes, a spacer arm sequence and a 3' end specific sequence P3 which is in complementary pairing with the specific sequence P1 of a corresponding supporting probe; and the supporting extended probes comprise amplification extended probes, wherein each amplification extension probe comprises a 5' end specific sequence P4 which can be combined with the target genes, a spacer arm sequence and a 3' end sequence P5; or the liquid phase chip also comprises a labeling probe in complementary pairing with the sequence P5. The liquid phase chip can perform hybridization reaction under the homogeneous reaction condition, and the designed probes have the advantages of high specificity and high signal to noise ratio during detection.
Owner:SUREXAM BIO TECH
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