31 results about "Hybridization reaction" patented technology

The invention discloses a composition, a detection method and a kit for SpaceAS variable splicing space in-situ detection of nucleic acid. The composition comprises a pi-type probe, a U-type probe, an O-type probe, an S-type probe and a signal probe, and the O-type probe adopts an in-vitro cyclization strategy, so that the problem of low hybridization efficiency caused by dependence on enzymatic cyclization reaction in a traditional hybridization method is effectively solved; meanwhile, an efficient probe hybridization system, a high-sensitivity signal amplification mechanism and a multi-round iterative imaging process are combined, so that the detection sensitivity and specificity are remarkably improved. The detection method has the advantages of high efficiency, high sensitivity, high specificity, good repeatability, strong expandability and the like through the synergistic effect of five-step single-stranded DNA probe hybridization reaction and one-step DNA amplification reaction, and can be widely applied to space in-situ detection and analysis of variable splicing events.

The invention relates to the technical field of molecular detection, in particular to a method, a system, a kit and a probe for detecting BRCA2 gene mutation. The detection method comprises the following steps: carrying out pretreatment and PCR amplification on a blood sample, carrying out a hybridization reaction with a probe to obtain a sample, detecting the sample based on an SERS technology, and collecting an SERS spectrum. Whether BRCA2 gene mutation exists or not is judged by identifying characteristic double peaks of a cy3 fluorophore at 1188 cm <-1 > and 1393 cm <-1 >, and the whole detection process can be completed within 2 hours. According to the scheme, the region, carrying the BRCA2 gene, on the DNA can be amplified through the specific primer pair, then the DNA is specifically combined through the improved oligonucleotide probe, and detection is achieved through the reporter molecule on the oligonucleotide probe; therefore, the detection method provided by the scheme has the advantages of high detection speed, high sensitivity and high detection result accuracy, and the problems of long time consumption and high cost of the traditional PCR or NGS technology are effectively solved.

The invention belongs to the field of inorganic functional materials, and particularly relates to a basic nickel carbonate modified material and a preparation method thereof.The method comprises the steps that nickel nitrate hexahydrate is dissolved in deionized water, urea and cetyltrimethylammonium bromide are added, the mixture is heated and stirred mildly, and stirring is performed to obtain a basic nickel carbonate modified material; then adding a specially-made polyaminophenylboronic acid nickel complex and a cobalt-nickel phosphate ethanediamine hybrid material, performing ultrasonic dispersion to form a mixed solution, and then performing hydrothermal reaction, centrifugal washing and vacuum drying to obtain a final product. The two special modified compounds are respectively prepared through a coordination reaction regulated by ammonia water and a hybridization reaction under high-temperature and high-pressure conditions. The modified material prepared by the method has a unique microstructure and enhanced physical and chemical properties, shows excellent application prospects in the fields of electrochemical energy storage, electro-catalytic water and environmental remediation, and particularly, the comprehensive performance of the material is remarkably improved due to the high specific surface area, abundant active sites and a stable structure.

The application discloses a new method for quantitatively analyzing interface nucleic acid hybridization reaction and application thereof, and comprises the following steps: S1, constructing a DNA tetrahedron interface; S2, imaging the DNA tetrahedron interface through a total internal reflection fluorescence microscope; S3, realizing real-time monitoring of a hybridization event of the DNA tetrahedron interface by adopting double-color co-localization and analyzing a fluorescence signal; and S4, analyzing hybridization kinetics on the DNA tetrahedron interface by recording changes of the fluorescence signal with time. The application uses the DNA tetrahedron interface for single-molecule fluorescence imaging, can realize real-time monitoring of a single nucleic acid molecule hybridization event, improve detection sensitivity and specificity, accurately analyze kinetic parameters of a hybridization reaction, help realize more accurate performance evaluation of a nucleic acid sensor, has important significance for design and optimization of the nucleic acid sensor, and thus plays a greater role in the fields of clinical diagnosis, environmental monitoring and food safety.

This invention provides a rapid hybridization reaction device for gene chips based on microfluidics, comprising a microfluidic layer based on microfluidic technology, a DNA probe microarray layer corresponding to the microfluidic channels in a dotted array, a temperature control module for providing the hybridization temperature between the DNA probes and DNA in the sample, and a fixing device that fixes and seals the microfluidic layer, DNA probe microarray layer, and temperature control module from top to bottom. Each microfluidic channel is in a continuous U-shaped configuration (positive-negative-positive-U), and is equipped with a liquid flow drive module for driving the DNA solution to flow back and forth within the microfluidic channel. After sealing, all probe array points are located within an independent microfluidic channel, and a sample dispensing orifice is provided at the upper end of the fixing device for adding samples to each independent microfluidic channel. Through the synergistic effect of temperature and flow rate, this device can reduce sample consumption, increase hybridization rate, reduce hybridization time, and avoid cross-contamination.

The invention discloses a library construction method for non-HPV (human papillomavirus) related cervical tumor and precancerous lesion molecular subtypes thereof based on high-throughput sequencing, which comprises the following steps: ultrasonically breaking genome DNA (deoxyribonucleic acid) of a detected sample to obtain a DNA fragment, modifying the tail end, connecting a joint, and performing purification and pre-amplification reaction, namely performing hybridization reaction on a pre-amplification sequence by using a hybridization capture reagent, according to the method, a hybridization capture reagent is adopted, DNA fragments combined through non-specific capture are enriched and removed, then an amplification reaction is carried out, a library is obtained, next-generation sequencing is carried out, original data are subjected to bioinformatics analysis, variation information is obtained, and the hybridization capture reagent comprises specific primers with the sequences shown as SEQ ID NO: 1-175. According to the present invention, the integration of the non-HPV-related cervical tumor and precancerous lesion molecule detection is achieved, the detection can be completed in one experiment, and the sequencing depth is improved by specifically enriching the target region so as to improve the sensitivity of the non-HPV-related cervical tumor and precancerous lesion molecule detection.

The application provides a biochip integrated machine, which comprises a shell and a hybridization part, a reading part and a driving part arranged in the shell. The hybridization part is provided with a liquid containing assembly and a liquid suction and discharge assembly. A reactor in which a biochip is arranged is detachably arranged in the hybridization part. The liquid containing assembly is used for containing liquid. The liquid suction and discharge assembly is connected with the reactor and the liquid in the liquid containing assembly is sucked into the reactor or the liquid in the reactor is discharged by applying pressure to the reactor. The driving part transmits driving force to the reactor to drive the reactor to move between the hybridization reaction area and the reading area. The reading part is used for reading the result of the hybridized biochip in the reading area. The application can integrate the biochip hybridization and the hybridization result reading, automatically complete the biochip hybridization reaction, the result display and acquisition, and has high working efficiency, simple and convenient operation, compact structure, flexible use and long service life.

The invention belongs to the technical field of library construction, and particularly relates to a DNA targeted library construction kit and application thereof. The kit comprises a hybridization capture reagent combination, the hybridization capture reagent combination comprises a hybridization reaction solution, and the hybridization reaction solution comprises a hybridization reaction solution 1 and a hybridization reaction solution 2; the hybridization reaction solution 1 is prepared from betaine, tetramethylammonium chloride, Mg < 2 + > and Triton X-100; the hybridization reaction liquid 2 is prepared from formamide and N-methyl pyrrolidone; hybridization of the probe and the library is facilitated, the capture efficiency is superior to that of an existing hybridization reaction solution, and Panel with different sizes and different hybridization durations can be compatible; a small panel can also achieve relatively high and relatively stable capture efficiency, the hybridization time is selectable within 1-20 h, fast and slow hybridization reagents are unified, and production and simultaneous operation of multiple products are facilitated.

The invention discloses an addressable nanoliter reactor chip and application of a nucleic acid signal amplification integrated system in single cell level detection markers. The nucleic acid signal amplification integrated system comprises aptamer DNA molecules and probe DNA molecules of a hairpin structure. According to the invention, the programmable characteristic of nucleic acid molecules is utilized to construct an aptamer-probe DNA synergistic signal amplification system; through combination of molecular beacon design and endonuclease reaction, in-situ signal multiplication of EpCAM protein is realized on a single cell level. After the aptamer recognizes the target epitope, the cascade hybridization reaction of the probe DNA is triggered, so that a single antigen binding event is converted into hundreds of fluorescent signal molecules to be released, and the signal gain is remarkably improved compared with that of a traditional immunofluorescence method.

The application discloses a PKR gene knockout cell line and a construction method and application thereof. The construction method comprises the following steps: A1, designing sgRNA primers for PKR gene knockout; A2, performing single enzyme cutting treatment on LentiCRISPR v2 plasmid to obtain enzyme cutting products; A3, performing annealing hybridization reaction by using the synthesized sgRNA primers to obtain annealing hybridization products; A4, performing a ligation reaction on the enzyme cutting products and the annealing hybridization products, and transforming the obtained reaction products into competent cells to obtain a recombinant transfer plasmid; and A5, after performing lentivirus packaging on the recombinant plasmid, infecting A549 cells by using the obtained lentivirus liquid to obtain the PKR gene knockout cell line. The application successfully constructs the A549 cell line with the PKR stable knockout, the A549 cell line can inhibit the type I interferon induced by NDV, promote virus replication and induce more serious cell death, and thus can be applied to the development of NDV vaccines.

This invention relates to an apparatus for producing small-molecule eco-organic products through biomass multi-component catalytic hydrolysis, comprising: a primary processing unit including replaceable staged crushing sub-equipment and pulping sub-equipment for adjustable and replaceable input; a secondary processing unit including a heating fermentation sub-equipment, a catalytic hydrolysis sub-equipment, and a nutrient element coupling hybridization sub-equipment connected in sequence, to obtain secondary biomass slurry from the primary biomass output from the primary processing unit through fermentation, catalytic hydrolysis, and nutrient element coupling hybridization reactions; a combined closed-loop circulation device and an intelligent control unit for controlling the particle size of the primary processing unit, coordinating the handling of various adjustable variables such as humidity, particle size, temperature, speed, pH, concentration, weight, pressure, liquid level, and time in each sub-equipment during the reaction process, and coordinating the handling of the primary and secondary processing units by the combined closed-loop circulation device.

The invention belongs to the technical field of library construction, and particularly relates to a hybridization reaction solution and application thereof. The invention provides a hybridization reaction solution, the capture efficiency of the hybridization reaction solution is superior to that of the existing hybridization reaction solution, and the hybridization reaction solution can be compatible with Panel with different sizes and different hybridization durations; a small panel can also achieve relatively high and relatively stable capture efficiency, the hybridization time is selectable within 1-20 h, fast and slow hybridization reagents are unified, and production and simultaneous operation of multiple products are facilitated.

The invention discloses a method and a kit for detecting gene polymorphism of MTHFR, MTRR and MTR. The method comprises the following steps: designing PCR (Polymerase Chain Reaction) amplification primer pairs of a C677T site of an MTHFR gene, an A66G site of the MTRR gene and an A2756G site of the MTR gene, and adding a marker at the 5'end of one primer of the primer pairs; a C677T site of an MTHFR gene, an A66G site of an MTRR gene and an A2756G site of an MTR gene are designed, one end of the C677T site of the MTHFR gene, one end of the A66G site of the MTRR gene and one end of the A2756G site of the MTRR gene comprise a specific capture probe complementary sequence, the other end of the C6677T site of the MTHFR gene comprise a detection probe complementary with a target nucleic acid characteristic sequence, carrying out hybridization reaction on the detection probe, the target nucleic acid fragment with the marker and a universal capture chip to form a sandwich composite structure; a marker signal is detected. The detection efficiency and sensitivity can be greatly improved, the operation is simple, convenient and rapid, and the cost is lower.

The application relates to a target nucleic acid capturing method and a detection method, which comprises the following steps: concentrating a nucleic acid library to be detected to obtain a nucleic acid library to be detected concentrate; mixing and dissolving the nucleic acid library to be detected concentrate with a probe group freeze-dried product to obtain a hybridization system, and performing a hybridization reaction; and after the hybridization reaction, adding capturing magnetic beads to capture the probe group, and obtaining a target nucleic acid library; wherein the probe group contains a base sequence which is at least partially complementary to the target nucleic acid. The method can improve the performance of hybridization capturing.

The invention provides a multiple miRNA detection electrochemical biosensor, a preparation method, a product and application, and belongs to the technical field of electrochemical detection. The multiple miRNA detection electrochemical biosensor is developed on the basis of an amino-functionalized nano material or a modified avidin nano material, and a specific capture probe and a signal probe marked with different metal ions or biotin are designed; by combining electrochemical detection methods such as two-step sandwich type hybridization reaction and differential pulse voltammetry or square wave voltammetry, quantitative simultaneous detection of multiple miRNAs is realized, the detection process is simplified, the detection efficiency is improved, and the problem that multiple markers cannot be simultaneously detected by a traditional method is solved. In addition, by optimizing hybridization reaction conditions and probe design, the specificity of the sensor is improved, cross reaction is reduced, meanwhile, the stability and reproducibility of detection results are guaranteed, and finally an efficient and accurate tool is provided for clinical detection of miRNA.

The invention discloses a method for promoting CAR (chimeric antigen receptor) aggregation by using DNA domino effect, and relates to the technical field of crossing of immunotherapy and biomechanics, the method comprises a trigger chain (Tr), a hairpin structure (HP1 / HP2) and a CAR-T cell, a multivalent DNA network structure is formed on the surface of the CAR-T cell through a hybridization chain reaction, the aggregation state of a CAR molecule is dynamically regulated, the trigger chain (Tr) is a single-stranded DNA molecule, and the hairpin structure (HP1 / HP2) is a hairpin structure (HP1 / HP2). CAR can be specifically recognized and a cascade hybridization reaction of a hairpin structure (HP1 / HP2) can be initiated, the hairpin structure (HP1 / HP2) is a partially complementary DNA sequence, a linear or branched DNA polymer is formed through the HCR reaction, and a CAR aggregation signal is expanded. According to the method for promoting CAR aggregation by using the DNA domino effect, controllable adjustment of CAR aggregation is realized through HCR, and mechanical properties of target cells are responded; and the DNA network structure obviously improves the mechanical force conduction efficiency of the CAR-T cells.

The application relates to the technical field of biological detection, and discloses an aflatoxin-producing gene detection method based on magnetic nanoparticles and carbon quantum dots, which comprises the following steps: mixing a to-be-detected sample containing target DNA and MNPs-capture probes, carrying out first incubation to generate base complementary pairing and combination; mixing the formed product and CQDs-signal probes, carrying out second incubation to generate hybridization reaction, so as to form a sandwich structure; separating the reaction system by using an external magnetic field, collecting supernatant after separation; testing the fluorescence intensity of the supernatant, and determining the concentration of the target nucleic acid according to the positive correlation between the fluorescence intensity reduction degree and the target DNA concentration. The application directly sets the fluorescence test object as the supernatant after separation by combining the magnetic separation technology with the double-probe sandwich method, eliminates the dissociation and elution steps of the probes in the conventional method, eliminates the optical interference of food matrix by using magnetic separation, simplifies the operation process, and improves the quantitative detection accuracy.

The application relates to an addition type silicone sealant tackifier and a preparation process thereof, and the preparation process comprises the following steps: S1, hydrolytic condensation reaction: mixing dialkoxysilane, vinyl-containing alkoxysilane, epoxy-containing alkoxysilane, methacryloyloxy-containing alkoxysilane, tetraethyl orthosilicate and an organic solvent, and performing a hydrolytic condensation reaction in the presence of an acid catalyst and water to obtain a polysiloxane oligomer intermediate; S2, titanate catalytic crosslinking: adding a titanate crosslinking agent and a hydrolysable inorganic nano-phase precursor into the polysiloxane oligomer intermediate, performing catalytic crosslinking and in-situ nano-hybridization reaction, neutralizing and refining to obtain the tackifier. The application simultaneously introduces a vinyl group, an epoxy group and a methacryloyloxy group, and through the action of titanate catalytic crosslinking and in-situ nano-hybridization reaction, the compatibility of the tackifier with the sealant is improved, and the tackifying effect and the aging-resistant mechanical property are unexpectedly improved.

The invention discloses a method for detecting an mRNA capping rate, and belongs to the technical field of biology. The detection method comprises the following steps: carrying out hybridization reaction on mRNA and a base-free modified probe, and carrying out enzyme digestion reaction on the product; and adding magnetic particles into the reaction product, incubating, cleaning and eluting to obtain the mRNA pretreatment product. According to the method, the unmodified probe is used for detecting the cap adding rate of the mRNA, the pretreatment process of the mRNA is mainly improved, and the reaction condition of hybridization combination of the probe and the mRNA is optimized, so that the higher detection fragment yield is obtained; by optimizing the elution mode, the proportion of the probe in a capping rate detection sample can be remarkably reduced, and the proportion of a target fragment is increased. Therefore, compared with a traditional detection method, the method has the advantages that the detection sensitivity can be improved, the detection lower limit is reduced, the resolution and accuracy are improved, the process is simplified, the cost can be reduced, and the application prospect is better.

The utility model relates to the field of chip production and processing equipment. The gene chip hybridization temperature control device comprises a chip container and further comprises a heating bottom plate, the heating bottom plate comprises a vapor chamber and a heater, the heater is installed below the vapor chamber, and the chip container is detachably connected to the upper portion of the vapor chamber; the chip container comprises a chip placing frame, a groove body with an opening in the upper end and a cover body, the upper end and the lower end of the chip placing frame are open, the opening in the upper end and the opening in the lower end of the chip placing frame are communicated with each other, the bottom of the groove body abuts against the opening in the lower end of the chip placing frame, and the cover body abuts against the opening in the upper end of the chip placing frame; a temperature measuring gap for measuring the temperature of liquid in the groove body is formed between the chip placing frame and the groove body; and the temperature measuring mechanism is used for being inserted into the temperature measuring gap. According to the utility model, the chip container is improved, the combination of the chip placing rack, the groove body and the cover body is adopted, and the temperature measuring gap is reserved, so that the hybridization reaction temperature can be conveniently detected.

This invention relates to the field of bioengineering technology and discloses a flow-through hybridization analysis method, apparatus, device, and medium. The method includes inkjet-spraying a paper-based chip to generate a circular spot array; wherein each circular spot is composed of several microdroplets; calculating the space-filling coefficient of the microdroplets; if the space-filling coefficient is less than a set threshold, optimizing the space-filling coefficient until it is not less than the threshold, resulting in a paper-based chip with uniformly distributed microdroplets; performing a flow-through hybridization reaction on the paper-based chip with uniformly distributed microdroplets to obtain a paper-based chip carrying a hybridization signal; and performing qualitative and quantitative detection and analysis on the paper-based chip carrying the hybridization signal to obtain the analysis results. This invention improves the detection accuracy of flow-through hybridization analysis and makes the signal distribution of the circular spots more regular, enhancing the readability of the results.

The invention discloses a library construction method for detecting gastrointestinal stromal tumor molecular typing and a gastrointestinal stromal tumor molecular typing method, and belongs to the technical field of biology. Comprising the following steps: fragmenting a DNA (Deoxyribonucleic Acid) extract of a gastrointestinal stromal tumor patient specimen, modifying a terminal, connecting a joint, purifying and pre-amplifying, and carrying out hybridization reaction on a hybridization capture enrichment probe and a pre-amplified sequence to enrich a target sequence; the hybridization capture enrichment probe comprises a sequence as shown in SEQ ID NO: 1-109; the method comprises the following steps of: performing amplification after non-specific capture and binding DNA fragments are eluted and removed, performing nucleic acid concentration and fragment length quantification on a product to obtain a library, and performing bioinformatics analysis on gastrointestinal stromal tumor patient specimen data by next-generation sequencing to obtain gastrointestinal stromal tumor molecular typing information. According to the invention, in-vitro non-diagnostic gastrointestinal stromal tumor molecular typing joint detection is realized, multi-gene variation form identification is completed in one experiment, the detection cost is saved, and the sensitivity is high.

The present invention relates to compositions and methods for detecting targets, and in particular to hybridization chain reactions for detecting nucleic acid targets. In some examples, the methods described herein include a looped hybridization reaction that induces modification of a polynucleotide linked to a detection moiety, enabling the detection moiety to generate a detectable signal.

The application discloses a quinolone antibiotic electrochemical biosensor and an electrochemical detection method. 2+ In the presence of quinolone antibiotics, the formation of quinolone antibiotic-Cu 2+ complexes will inhibit the specific DNA ribozyme (Cu 2+ -DNAzyme) activity, resulting in the retention of the intact substrate strand probe on the electrode surface, and then triggering the chain hybridization reaction (HCR), forming long double-stranded DNA nanowires on the electrode surface, signal amplification, and the finally generated electrochemical signal being positively correlated with the concentration of the target quinolone antibiotic. The electrochemical sensor of the application is simple to operate, has outstanding sensitivity and specificity, and is successfully applied to the detection of ciprofloxacin in actual food samples, and has potential practicability in food safety detection.

The present disclosure provides a probe set, reagent, kit and method for detecting gene mutation. Specifically, the detection method comprises: fragmenting sample sequences and denaturing; hybridizing the denatured sample sequences with at least one probe set; wherein the probe set comprises a first probe and a second probe; performing an extension reaction of the first probe on the hybridization product; connecting the extension product with the second probe to obtain a sequencing sequence for at least one target region; wherein the number of target regions corresponds to the number of probe sets; amplifying and enriching the sequencing sequence, and sequencing the enrichment product. Such technical solution not only has a simple and easy-to-operate process, but also can flexibly design the probe set, is suitable for target genes of various sizes, has high specificity and low cost.

This invention discloses an organic-inorganic hybrid material based on straw biomass and its preparation method, relating to the fields of environmental functional materials and waste resource utilization technology. The organic-inorganic hybrid material is formed by chemical bonding between pretreated straw fibers and activated inorganic clay minerals through a crosslinking agent, constituting an organic-inorganic interpenetrating three-dimensional network structure. It is prepared through straw pretreatment, inorganic phase activation, hybridization reaction, and shaping and drying steps. The chemical bonding includes at least one of covalent bonds, coordinate bonds, and hydrogen bonds. The mass ratio of straw fibers to inorganic clay minerals is 1:0.5–5. This organic-inorganic hybrid material forms a stable three-dimensional network through chemical bonding, improving the material's mechanical strength and durability, solving the interfacial compatibility problem, and avoiding phase separation. Its preparation method is simple, easily scalable, has wide applications, realizes waste resource utilization, reduces environmental burden, and is low-cost.

This invention discloses a method, system, computer equipment, and readable storage medium for signature image DNA encoding storage and anti-counterfeiting verification based on feature learning, belonging to the field of electronic information technology. The method includes: extracting high-dimensional discriminative feature vectors from the signature image to be verified using a feature extractor; inputting the vectors into a trained sequence encoder to generate a DNA sequence; synthesizing a reverse complementary sequence to prepare a labeled probe; hybridizing the probe with the registered DNA sequence of the target signer; measuring the hybridization yield; if the yield exceeds a preset threshold, the signature is determined to be genuine; otherwise, it is a forged signature. This invention is the first to integrate handwritten signature anti-counterfeiting with DNA storage. Through three core components—a feature extractor, a sequence encoder, and a hybridization predictor—and multi-task joint training, genuine signature pairs are encoded as high-hybridization-yield sequences, while genuine and forged signature pairs are encoded as low-hybridization-yield sequences, achieving physical-level anti-counterfeiting verification based on molecular hybridization. The verification process of this invention is inherently parallel, energy-efficient, and highly accurate, meeting the needs of practical anti-counterfeiting applications.

The invention discloses a nucleic acid hybridization capture method and a kit, and the method comprises the following steps: constructing a hybridization reaction system, hybridizing the 5 '-phosphorylated probe to the nucleic acid sample wherein the 5'-portion and the 3 '-portion of the probe are complementary to a first region and a second region, which are not adjacent, in the nucleic acid sample, respectively, and a backbone portion connecting the 5'-portion and the 3 '-portion is not complementary to the nucleic acid sample; reacting the hybrid product under the action of nucleic acid polymerase and / or a polymerase active fragment, and nucleic acid ligase and / or a ligase active fragment to obtain a looping probe; and enriching the ring forming probe. Compared with the prior art, the method disclosed by the invention has the advantages of high specificity, high sensitivity, high flexibility, short process, convenience in operation, wide application scene and the like, and has a good application prospect.

This invention provides a capture probe set for whole-genome sequencing of hepatitis A virus (HAV). The nucleotide sequences of the capture probe set are shown in SEQ ID No:1 to SEQ ID No:880. This capture probe set can be used to prepare kits for whole-genome sequencing of HAV. This invention also provides a method for whole-genome sequencing of HAV using the capture probe set. The method involves liquid-phase hybridization of a total RNA library from a sample to be tested with the capture probe set. DNA fragments hybridized with the capture probe set are isolated from the hybridization reaction solution. These DNA fragments are then used as templates for capture-and-PCR amplification. The PCR products are sequenced, and after data analysis, the full-length HAV genome sequence is obtained. The capture probe set designed in this invention can capture the HAV genome. By optimizing the probe length, it can specifically enrich ultra-low copy numbers of the viral genome from high-background host nucleic acids, showing broad application prospects.