445 results about "Hybridization reaction" patented technology

This invention provides an apparatus and method for analyzing a polynucleotide sequence; either an unknown sequence or a known sequence. A support, e.g. a glass plate, carries an array of the whole or a chosen part of a complete set of oligonucleotides which are capable of taking part in hybridization reactions. The array may comprise one or more pair of oligonucleotides of chosen lengths. The polynucleotide sequence, or fragments thereof, are labelled and applied to the array under hybridizing conditions. Applications include analyses of known point mutations, genomic fingerprinting, linkage analysis, characterization of mRNAs, mRNA populations, and sequence determination.

Devices, apparatus and methods are disclosed for carrying out processing steps involved in chemical reactions such as hybridization reactions conducted on the surface of a substrate comprising chemical compounds such as biopolymer features. A device comprises a housing comprising a housing chamber. The housing comprises an opening through which a substrate may be inserted into the housing chamber. The device may also comprise a lift mechanism for moving the substrate in and out of the housing chamber in a controlled manner. At least one inlet may be in fluid communication with the housing chamber and at least one outlet may be in fluid communication with the housing chamber. Also disclosed are methods wherein the surface is brought into contact with a processing fluid; and, then, the surface is removed from contact with the fluid in a controlled manner at a rate that substantially eliminates droplet formation of the fluid on the surface of the substrate.

The invention relates to a method for modifying the surface of carbon fiber coated with nano sol through plasma treatment, which comprises the following steps: firstly, nano particles are prepared into an organic solvent, a sol solution of water or a sol solution prepared by hybridization reaction of a precursor solution of organic-inorganic nano particles by the ultrasonic vibrating technology; secondly, the sol solution is coated on the surface of the carbon fiber, treated by means of spray coating and padding, and dried; and thirdly, the dried carbon fiber is placed on a special transport unit for plasma processing equipment and a plasma is sprayed on the surface of the carbon fiber to make the carbon fiber move in the plasma atmosphere, so as to generate surface modification, wherein the treating power is between 10 and , watts, and the treatment time is between 0.5 and 300 seconds. The method can effectively improve the performance of the fiber, improves the molded manufacturability and the overall properties of composite materials of the fiber, has simple technology, quick processing speed, good treatment effect and low cost, is convenient to operate and difficult to cause environmental pollution, can reduce energy consumption, and is suitable for industrial production.

A novel hybridization device that improves the efficiency and consistency of microarray hybridization reactions by achieving a greater degree of internal mixing of target solution. The device provides a gasket-and-cover-type chamber wherein solution mixing is achieved by the creation of a multitude of microbubbles. One or more of the inner walls that define the chamber contain bubble-rupturing elements that extend into the chamber and terminate in sharp edges. They are typically located on opposite sides of a rectangular chamber and are pointed in a direction opposing bubble movement. Their interference with larger bubbles causes their breakup into microbubbles which travel separate and distinct paths as a result of external agitation and thereby provide improved solution mixing that results in a uniform distribution of target molecules to the probe molecules bound to the substrate. The sensitivity and consistency of the hybridization reaction is significantly increased.

The invention provides a method of synthesizing complex mixtures of long polynucleotides by separately synthesizing and assembling shorter component oligonucleotides. In one aspect, pairs of oligonucleotides that form components of such polynucleotides are synthesized on one or more microarrays, or other large-scale parallel solid phase synthesis platforms, after which they are released. Members of each pair contain unique complementary barcode sequences that are used match-up pairs in a hybridization reaction to form duplexes. Such duplexes are then extended with a DNA polymerase and the resulting extension product is amplified to form an amplicon. The amplicon may be either used directly as the desired polynucleotide, or it may undergo further processing, such as capture on solid phase supports and/or additional enzymatic or chemical processing, to produce a desired polynucleotide product, such as a circularizing probe for multiplex analysis of genomic DNA, or the like.

The invention relates to a rolling circle amplification-based colorimetric assay method for target nucleic acids or proteins. The method mainly utilizes the hybridization between nucleic acids and antigen-antibody binding to indirectly fix the target nucleic acids or proteins to be assayed onto magnetic beads, rolling circles are added, a rolling circle primer on hybrid is utilized to carry out constant-temperature rolling circle amplification, and finally, the chromogenic reaction of nanogold is utilized to assay constant-temperature amplified macromolecular product in order to achieve the purpose of relatively assaying the target nucleic acids and proteins. By integrating rolling circle amplification and the chromogenic reaction of nanogold, the method not only amplifies a large quantity of micro-sample signals, but also realizes the rapid, visual assay of results, avoids the usage of a PCR (Polymerase Chain Reaction) instrument and other expensive instruments, and decreases the requirement on the experimental hardware condition. The method is expected to be applied in bedside rapid diagnosis and the field rapid assay of microorganisms in food safety and the environment.

Biological species are determined easily, inexpensively, in a short time, and accurately even if a plurality biological species having base sequences similar to one another exist in a specimen. For achieving such an object, the information processing apparatus according to the present invention is an information processing apparatus processing information about the signal intensity of each probe obtained as a result of making a predetermined specimen undergo a hybridization reaction using a DNA micro-array in which probes being nucleic acid complementary to some of nucleotide sequences of biological species, the information processing apparatus comprising unit configured to retain a known sample, unit configured to acquire an unknown sample obtained as a result of making the predetermined specimen undergo the hybridization reaction, unit configured extract a vector related to a vector related to a predetermined biological species, of the known sample and unknown sample, and determining unit configured to compare the extracted vector of the known sample with the vector of the unknown sample to determine whether or not the predetermined biological species is contained in the predetermined specimen.

The invention discloses a method of detecting adenosine with a fluorescent sensor on the basis of an aptamer. In the method, gold nano particles modified with the aptamer are used as a recognition probe and an energy acceptor, and carbon dots modified with an aptamer complementary chain are used as a fluorescent probe and an energy donor; through a hybridization reaction, fluorescence resonance energy transfer is carried out between the gold nano particles and the carbon dots, and fluorescence of the detection system is quenched. After addition of adenosine, the adenosine and the aptamer complementary chain are competitively combined with the aptamer, so that energy transfer efficiency between the gold nano particles and the carbon dots is weakened; and the fluorescence of the detection system is recovered, so that based on the change of the fluorescence signal, the method achieves quantitative detection of the adenosine. The method has strong specificity and simple operation, is low in required quantity of a sample, is high in sensitivity, can be used for detecting the adenosine in a blood sample and supplies useful analysis data for clinical diagnosis on diseases.

The invention provides a heat radiation material, a heat radiation structure, and preparation methods thereof. The heat radiation material comprises, by weight, 10-30 parts of inorganic heat radiation nano-grade material aqueous slurry, 40-80 parts of aqueous high-molecular resin, 0.5-5 parts of an auxiliary agent, and 5-20 parts of a diluting agent. The inorganic heat radiation nano-grade material aqueous slurry comprises, by weight, 10-25 parts of an inorganic heat radiation nano-grade material, 0.5-20 parts of a bi-functional large-molecular modifier, and 50-100 parts of a solvent. According to the inorganic heat radiation nano-grade material, the bi-functional large-molecular modifier is used in surface modification. Selective absorption and grafting hybridization reaction are carried out on the surface of the inorganic heat radiation nano-grade material, such that coordination self-assembly behaviors of ester bond, silicon-oxygen bond, hydrogen bond, and the like are formed on the surface of the material. Therefore, inorganic heat radiation nano-grade material interface performance is controlled, compatibility and system dispersion stability of the inorganic heat radiation nano-grade material are improved, and better heat radiation performance can be obtained.

The invention discloses a method for detecting miRNA (ribonucleic acid). The method comprises the steps: (1) synthesizing a DNA (deoxyribonucleic acid) tetrahedron probe; (2) connecting three peak points of the DNA tetrahedron probe to the surface of a working electrode of an electrochemical device to obtain a working electrode with a capture probe; (3) adding a target miRNAs to be detected, a signal probe and an auxiliary chain into a reaction system to implement hybridization reaction to form a composite body I, and immersing the working electrode into the reaction system to implement hybridization reaction to form a composite body II; (4) generating reaction between the composite body II and enzyme capable of generating catalytic oxidation reduction reaction; (5) adding a primer produced by the enzyme catalytic oxidation to realize electrochemical detection analysis. The target miRNAs can be directly detected by the method disclosed by the invention without being marked and subjected to pre-PCR (polymerase chain reaction) multiplication; the method is easy to operate, so that the experiment cost is greatly reduced, and the experiment efficiency is improved.

The invention relates to a method for modifying a nanometer sol ultrahigh molecular weight polyethylene fiber by plasma treatment. The method comprises that: (1) inorganic nanometer particle is prepared into a sol solution by an ultrasonic shock technique; or (2) a precursor solution of the organic-inorganic nanometer particle is subjected to hybridization reaction with the inorganic nanometer particle to prepare the sol solution; (3) the sol solution is coated on the surface of the ultrahigh molecular weight polyethylene fiber; and (4) the ultrahigh molecular weight polyethylene fiber is dried at a temperature of 10 and 150 DEG C, and the solvent is collected; the ultrahigh molecular weight polyethylene fiber is introduced in a plasma atmosphere region by a plasma generator for plasma surface modification; and the treated ultrahigh molecular weight polyethylene fiber is on-line rolled by an automatic rolling machine. The compound property between the treated ultrahigh molecular weight polyethylene fiber and the organic matrix materials is greatly improved; moreover, the method also has the advantages of simple process, good treatment effect, low cost, environmental protection and low energy consumption.

The invention discloses a novel electrochemical DNA sensor constructed by a nanogold and partially-reduced graphene oxide (p-RGO) modified ionic liquid carbon paste electrode (CILE) as a platform prepared by an electrochemical method, and a method of applying the CILE to detect the Listeria feature gene sequence. 1-hexylpyridiniumhexafluorophosphate as a modifier is used for preparing the substrate electrode CILE, gold nanoparticles are deposited on the surface of the substrate electrode CILE and then the p-RGO is prepared by controlling electrochemical reduction conditions; an amino-modified probe ssDNA is fixed on the surface of the electrode by an amido bond covalent bonding method through carboxyl groups remained on the surface of p-RGO to constitute ssDNA/p-RGO/AuNPs/CILE; methylene blue (MB) as an indicator is used for detecting the hybridization reaction after a target sequence is hybridized, and a differential pulse voltammetry (DPV) is used for detecting the Listeria feature gene segment.

The invention discloses a preparation method for high-hardness waterborne polyurethane dispersion. An inorganic nano material and waterborne polyurethane are grafted through an in-situ polymerization method, so that the hybridization reaction of inorganic materials and the organic materials is realized and complementary advantages are realized. The preparation method comprises the following operation steps: adding polyisocyanate, macromolecule polyol, a catalyst, a chain extender and a silane coupling agent according to certain weight ratio in a flask for reacting to obtain a polyurethane prepolymer; after the reaction is ended, sequentially adding a neutralizer, inorganic nano material dispersion liquid and deionized water under the condition of low-temperature and high-speed stirring; finally, heating under the vacuum condition for removing the solvent to obtain the high-hardness waterborne polyurethane dispersion. Compared with the traditional inorganic and organic blending process, the preparation method has the characteristics that products prepared through the preparation method have the advantages of good stability, excellent water resistance, long-time aging resistance, excellent and high adhesion, high drying speed, high hardness, good fullness, higher light transmittance and the like, and are wide in application range.

The invention provides a coating for building glass heat insulation which can release negative ions and a preparation method; the coating comprises inorganic heat-insulation nano material, siloxane polymer, inorganic heat-insulation nano material aqueous slurry of solvent, negative ion powder material aqueous slurry, aqueous resin, assistant and diluent; the inorganic heat-insulation nano material adopts siloxane polymer with discrete and exquisite structure as a template, selective adsorption and grafting hybridization reaction are carried out on the surface of the inorganic heat-insulation nano material, silicon-oxygen bond and hydrogen bond coordination self-assembly action is formed on the surface of the heat-insulation nano material to control the interface performance of the heat-insulation nano material, improve the compatibility and the system dispersion stability among the heat-insulation nano materials and obtain special optical and heat-insulation performances; in addition, the synergistic effect among the negative ion material, the inorganic heat-insulation nano material and the aqueous resin can improve the heat-insulation effect and the capacity of releasing the negative ions, so as to realize multi-functionalization of the building glass heat-insulation coating.

The present invention relates to protein chip based on labeling streptavidin-biotin technology. The hybridization reaction includes the following steps: dropping tested sample to sample applied chip and washing out redundant sample with elution liquid; blocking wtih blockage liquid, washing and air drying; dropping protein marked with biotin diluted by blockage liquid, washing and air drying; anddropping fluorescnet or enzyme labeled streptavidin diluted by blockage liquid, washing and air drying. The protein chip of the present invention is used in determining antigen and antibody, and detecting specific matter combined with protein. The improved method of the present invention has clear, stable and reliable test results and wide application range.

The invention belongs to the field of chemiluminescence sensors, and relates to a method for determining melamine (Me), which is implemented by using a target induced chain releasing technique and combining with a hybrid chain reaction and a chemiluminescence technique. When a target Me exists, the Me acts with aptamer DNA1 (deoxyribonucleic acid 1) in double-stranded DNA on the surface of a functionalized magnetic bead (FMB1) so as to release a complementary sequence DNA2 of the aptamer; and the released complementary sequence DNA2 acts with another functionalized magnetic bead (FMB2), and a chain hybridization reaction is performed in the presence of hairpin DNA (HRP-H1 and HRP-H2) for modifying HRP, so that the two kinds of modified DNA grow into long double-stranded DNA. Through magnetic separation, and by using the catalytic action of HRP on a luminol-H2O2 chemiluminescence system, chemiluminescence is produced, and then the determination of the Me is realized through determining the chemiluminescence intensity. The method is high in selectivity and detection sensitivity.

The invention relates to a method for modifying Kevlar fiber by treating a nano sol through plasma, which comprises the following steps: (1) preparing inorganic nano particles into sol solution by ultrasonic oscillation technology; or (2) carrying out a hybridization reaction of precursor solution of organic-inorganic nano particles and the inorganic nano particles to prepare sol solution; (3) coating the sol solution on the surface of the Kevlar fiber; (4) drying the Kevlar fiber at a temperature of between 10 and 150 DEG C, collecting solvent, then introducing the Kevlar fiber in a plasma atmosphere area through a plasma generating device for surface modification treatment by the plasma; and finally, carrying out on-line winding of the treated Kevlar fiber in an automatic winding machine and adjusting the wiring speed of the Kevlar fiber by adjusting the speed range of a winding shaft. The combined performance between the Kevlar fiber treated by the method and the organic base materials is improved greatly; and the method has simple process, good treatment effect, low cost, difficult environmental pollution and reduction of the energy consumption.

A nucleic acid automatic examining device implements a hybridization reaction step after implementing a nucleic acid amplification reaction step on a nucleic acid sample containing a target nucleic acid. The device includes a nucleic acid amplification unit, a hybridization reaction unit, a dispenser unit for moving the nucleic acid sample from the nucleic acid amplification unit to the hybridization reaction unit, and a detector for detecting presence or absence of a reaction cassette in the hybridization reaction unit. When the detector detects the absence of the reaction cassette, the device implements steps up to the nucleic acid amplification reaction step and the dispenser unit is stopped so that the device may not implement the subsequent hybridization reaction step.

The invention belongs to the field of detecting a trace amount of ions in the water environment, and particularly relates to a visualization method for rapidly detecting a trace amount of uranyl ions in the water environment. The method mainly includes the steps that DNAzyme with the specific recognition function on UO2 <2+> is fixed to the surfaces of magnetic beads, and horse radish peroxidase is preassembled on the surface of nano-gold; then the magnetic beads are connected with the nano-gold through the cutting effect of the UO2<2+> on the DNAzyme and the hybridization reaction of DNA, after separation and collection are carried out through an external magnetic field, H2O2 oxidation tetramethyl benzidine is efficiently catalyzed through the horse radish peroxidase to enable a solution to be changed from the blank to the blue, and therefore sensitive and specific visualization rapid detection of the UO2<2+> ions is achieved. As the method has the advantages of being high in sensitivity, high in specificity, high in matrix interference resistance, simple, rapid, low in cost and the like, the method can be used for site rapid visualization detection of the trace amount of UO2<2+> ions in various water samples.

The invention provides a single-wall carbon nano tube-based ultrasensitive deoxyribonucleic acid (DNA) biosensor and a preparation method and application thereof. In the method, single-wall carbon nano tubes (SWCNTs) are grown on the surface of a silicon wafer on site by a chemical vapor deposition method, and gold nano particles are deposited on the surfaces of carbon nano tube electrodes by an electrochemical deposition technology. A single-stranded DNA (ssDNA) probe is self-assembled to the surfaces of SWCNTs-Au electrodes and subjected to hybridization reaction with complementary ssDNA. The change of electron transfer resistance before and after hybridization is recorded by utilizing the unique specific surface areas of the carbon nano tubes and the quick dynamic characteristics of the electrodes and by an electrochemical impedance method under the action of current signal amplification of nanometer gold to realize the quantitative detection of the complementary DNA. The detection limit of the sensor on the target DNA can reach between 10 and 20 M, so the sensor has the advantages of high sensitivity and selectivity, capability of being used repeatedly and the like, and has important significance in fields of medical diagnosis, the food industry, environment friendliness and the like.

The invention discloses a liquid phase chip for detecting breast cancer prognosis-related gene mRNA expression level, which mainly comprises a microsphere which aims at different target genes and is coupled to amido modified supporting probes, supporting extended probes connecting the supporting probes and target gene mRNA and amplification extended probes. Each supporting probe mainly comprises a 5' end spacer arm sequence and a 3' end specific sequence P1 which is in complementary pairing with a supporting extended probe; the supporting probe comprises SEQ. ID NO.1 specific to ESR1gene and SEQ. ID NO.2 specific to PGR gene; each supporting extended probe mainly comprises a 5' end specific sequence P2 which can be correspondingly combined with the target genes, a spacer arm sequence and a 3' end specific sequence P3 which is in complementary pairing with the specific sequence P1 of a corresponding supporting probe; and the supporting extended probes comprise amplification extended probes, wherein each amplification extension probe comprises a 5' end specific sequence P4 which can be combined with the target genes, a spacer arm sequence and a 3' end sequence P5; or the liquid phase chip also comprises a labeling probe in complementary pairing with the sequence P5. The liquid phase chip can perform hybridization reaction under the homogeneous reaction condition, and the designed probes have the advantages of high specificity and high signal to noise ratio during detection.