Method for determining melamine (Me) by chemiluminescence

A melamine and chemiluminescence technology, which is applied in the field of analytical chemistry and chemiluminescence sensors, can solve the problem of low sensitivity

Inactive Publication Date: 2013-12-11
广州倍腾新材料有限公司
View PDF6 Cites 21 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have their own advantages, and can meet the detection requirements of melami...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for determining melamine (Me) by chemiluminescence
  • Method for determining melamine (Me) by chemiluminescence
  • Method for determining melamine (Me) by chemiluminescence

Examples

Experimental program
Comparison scheme
Effect test

example

[0030] Example: A method for the determination of melamine based on target-induced chain release and hybridization chain reaction chemiluminescence

[0031] 1. Experimental part

[0032] 1.1 Instruments and reagents

[0033] 1-(3-Dimethylaminoaldehyde)-3-ethyldiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) were purchased from Tianjin BASF Chemical Co., Ltd. Luminol (luminol) was purchased from Shanghai Aladdin Reagent Company. HRP (horseradish peroxidase) was purchased from Shanghai Shunshi Biotechnology Co., Ltd.

[0034] The artificially synthesized DNA sequence used was purchased from Beijing Saibaisheng Bioengineering Co., Ltd., and the sequence is as follows:

[0035] The partial sequence of DNA1 is: 5′-TTT TTT TTT TTT CCA AAA GGG-(CH 2 ) 6 -SH-3′

[0036] The partial sequence of DNA2 is: 5′-TGG AAA AAA AAA AAA-3′

[0037] The partial sequence of H1 is: 5'-NH 2 -(CH 2 ) 6 -GCG AGG TTT TTT TTT TTT CCA GCG CCG CAC AGA TGG AAA AAA AAA AAA-3′

[0038] T...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of chemiluminescence sensors, and relates to a method for determining melamine (Me), which is implemented by using a target induced chain releasing technique and combining with a hybrid chain reaction and a chemiluminescence technique. When a target Me exists, the Me acts with aptamer DNA1 (deoxyribonucleic acid 1) in double-stranded DNA on the surface of a functionalized magnetic bead (FMB1) so as to release a complementary sequence DNA2 of the aptamer; and the released complementary sequence DNA2 acts with another functionalized magnetic bead (FMB2), and a chain hybridization reaction is performed in the presence of hairpin DNA (HRP-H1 and HRP-H2) for modifying HRP, so that the two kinds of modified DNA grow into long double-stranded DNA. Through magnetic separation, and by using the catalytic action of HRP on a luminol-H2O2 chemiluminescence system, chemiluminescence is produced, and then the determination of the Me is realized through determining the chemiluminescence intensity. The method is high in selectivity and detection sensitivity.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and chemiluminescence sensors, and relates to a method for measuring melamine established by using target-induced chain release technology combined with hybridization chain reaction and chemiluminescence measurement technology. Background technique [0002] Melamine (Chemical formula: C 3 h 6 N 6 ), commonly known as melamine, protein essence, IUPAC named: 1,3,5-triazine-2,4,6-triamine, is a triazine nitrogen-containing heterocyclic organic compound, used as a chemical raw material. Harmful to the body, not used in food processing or food additives. [0003] Currently developed melamine detection methods include ELISA (Choi J H, Kim Y T, Lee J H. Rapid quantification of melamine in milk using competitive 1,1'-oxalyldiimidazole chemiluminescent enzyme immunoassay. Analyst, 2010, 135:2445), mass spectrometry Method (Yang S P, Ding J H, Zheng J. Detection of melamine in milk products by surfa...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/76C12N15/11
Inventor 混旭
Owner 广州倍腾新材料有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap