Methods of producing homogeneous plastic-adherent aptamer-magnetic bead-fluorophore and other sandwich assays

A sandwich analysis, fluorophore technology, applied in biochemical equipment and methods, microbial determination/inspection, biological testing, etc., can solve problems such as not very good effect, not very good adhesion effect, etc.
CN102165071AInactive Publication Date: 2011-08-24OTC BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
OTC BIOTECH
Publication Date
2011-08-24
Estimated Expiration
Not applicable · inactive patent

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Abstract

Methods are described for assembly of DNA aptamer-magnetic bead ('MB') conjugate plus aptamer-quantum dot ('QD') aptamer-fluorescent nanoparticle or other aptamer-fluorophore, aptamer-chemiluminescent reporter, aptamer-radioisotope or other aptamer-reporter conjugate sandwich assays that enable adherence to glass, polystyrene and other plastics. Adherence to glass or plastics enables detection of surface-concentrated partitioning of fluorescence versus background (bulk solution) fluorescence in one step (without a wash step) even when the external magnetic field for concentrating the assay is removed. This assay format enables rapid, one-step (homogeneous) assays for a variety of analytes without wash steps that do not sacrifice sensitivity.
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Description

[0001] Cross References to Related Applications

[0002] This application is based on, and claims priority from, US Provisional Application Nos. 61 / 066,506 and 61 / 132,147, the entire contents of which are incorporated herein by reference. technical field

[0003] The present invention relates to the related fields of aptamer-based and nucleic acid-based diagnostic reagents. More specifically, the present invention relates to the preparation of self-assembling DNA aptamer-magnetic bead ("MB") conjugates or other aptamer-fluorophore conjugate sandwich analytes conjugated to aptamer-quantum dots ("QD") and methods of use wherein the analyte naturally adheres to glass and certain plastics such as polystyrene (or its derivatives) for one-step (homogeneous) detection without washing, even after removal of external magnetic beads No washing required. The binding between aptamers and magnetic beads or quantum dots and other fluorophores can be achieved through simple chemical bindin...

Claims

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