466 results about "Lipopolysaccharide" patented technology

The invention relates to the technical field of microorganisms, in particular to application of lactobacillus rhamnosus KF7 in improving intestinal barrier injury and regulating intestinal flora and functional food containing the lactobacillus rhamnosus KF7. The invention provides application of lactobacillus rhamnosus KF7 in preparation of a product for improving intestinal barrier injury and / or regulating intestinal flora. The preservation number of the lactobacillus rhamnosus KF7 is CGMCC (China General Microbiological Culture Collection Center) NO.6430. The invention further provides application of the lactobacillus rhamnosus KF7 in preparation of a product for improving intestinal barrier injury and / or regulating intestinal flora. The invention discloses the novel application of the caseus rhamnosus KF7 in the aspects of improving the normal intestinal epithelial barrier function, protecting the normal intestinal epithelial barrier from lipopolysaccharide induced barrier injury, recovering animal intestinal barrier injury caused by rhythm disorder, regulating intestinal flora disorder and improving growth, development and metabolic states for the first time. The method can be used for producing functional food products such as fermented milk, lactic acid bacteria beverages, probiotic powder, viable bacteria water, milk powder and cheese.

The invention discloses application of scylla serrata antibacterial peptide Scycin in preparation of an anti-inflammatory composition and a preparation method of the scylla serrata antibacterial peptide Scycin. The nucleotide sequence of the scylla serrata antibacterial peptide Scycin is shown as SEQ ID NO.01. The invention further discloses a preparation method of the scylla serrata antibacterial peptide Scycin. In an LPS (lipopolysaccharide)-induced RAW 264.7 macrophage inflammation model, the recombinant expression plasmid vector containing the scylla antibacterial peptide Scin remarkably inhibits an inflammation cascade reaction by down-regulating expression of proinflammatory factors such as TNF-alpha (tumor necrosis factor-alpha) and IL-6 (interleukin-6), the anti-inflammatory effect of the recombinant expression plasmid vector is equivalent to that of a mesalazine positive control group, and obvious cytotoxicity is not observed.

The invention discloses a fish by-product active peptide, a preparation and application, and belongs to the technical field of biological active peptides. The amino acid sequences of the fish by-product active peptide are shown as SEQ ID NO: 1 to SEQ ID NO: 3. Both the single peptide fragment and the compound peptide fragment of the fish by-product active peptide can obviously inhibit NO secretion of a lipopolysaccharide (LPS)-induced macrophage (RAW264.7) inflammation model, so that the fish by-product active peptide has a relatively good application prospect in preparation of a product with an anti-inflammatory effect; and a foundation is laid for high-added-value utilization of salmon byproducts and promotion of research, development and application of functional active peptides.

The invention discloses an application of phenyllactic acid or a composition containing phenyllactic acid in preparation of medicines for relieving lung diseases, and in the application, the lung diseases are preferably pulmonary fibrosis or acute lung injury. In an acute lung injury research model, phenyllactic acid can effectively intervene in the inflammatory factor cascade reaction and oxidative stress injury process, and acute lung injury symptoms caused by the factors are remarkably improved. Compared with the traditional treatment means, the experiment proves that the phenyllactic acid or the composition containing the phenyllactic acid can obviously relieve the symptoms of acute lung injury (ALI) of mice induced by lipopolysaccharide (LPS) and pulmonary fibrosis (PF) of mice induced by bleomycin, the pathological change of the lung is effectively improved, and the lung function is recovered.

The invention provides a compound probiotic for relieving acute lung injury and application of the compound probiotic, and particularly discloses application of the compound probiotic consisting of bifidobacterium animalis YRK-12 and lactobacillus rhamnosus LRa-Y8 in preparation of a medicine for relieving the acute lung injury. Wherein the preservation number of the bifidobacterium animalis YRK-12 is CGMCC (China General Microbiological Culture Collection Center) No.28597; the preservation number of the lactobacillus rhamnosus LRa-Y8 is CGMCC (China General Microbiological Culture Collection Center) No. 34186. Experimental verification shows that the compound probiotics can remarkably relieve acute lung injury induced by lipopolysaccharide (LPS) through a synergistic effect; specifically, the weight loss rate of mice is reduced, pathological injuries of lung tissues are improved (the structural integrity of alveolar alveoli is maintained, and inflammatory cell infiltration is reduced), the levels of proinflammatory factors (IL-1beta, TNF-alpha and IL-6) in serum and lung tissues are reduced, and the level of anti-inflammatory factors (IL-4) is increased, so that the immune balance of the lung is regulated through an intestine-lung axis; and a safe and effective novel micro-ecological regulation strategy is provided for prevention and treatment of acute lung injury.

The invention discloses a lipopolysaccharide response type nano antibacterial peptide as well as a preparation method and application thereof, and belongs to the technical field of biology, and the amino acid sequence of the antibacterial peptide is as shown in SEQ ID No.1. Meanwhile, the invention further discloses application of the antibacterial peptide in preparation of drugs for treating gram-negative bacterium infectious diseases. The antibacterial peptide disclosed by the invention can be combined with lipopolysaccharide in a targeted manner to form nanofibers for capturing, and then the nanofibers are clustered and presented to phagocytes for phagocytosis, so that killing of beneficial bacteria in a host is fully avoided. In conclusion, the antibacterial peptide disclosed by the invention can target gram-negative bacteria to rupture membranes and sterilize, fully avoid mistakenly killing beneficial bacteria in a host body and effectively reduce the generation of drug resistance, so that the antibacterial peptide is a nano capture peptide with an immune regulation function, which has great development potential and relatively high application value.

The invention discloses a tumor whole cell wrapped by a metal-polyphenol nano coating as well as a preparation method and application thereof. The preparation method comprises the following steps: step 1, fully mixing a plant polyphenol solution and a tumor cell suspension; 2, adding a manganese ion-containing compound solution, and fully mixing; 3, adding a buffer solution into the solution obtained in the step 2, fully mixing, washing, and centrifugally separating to obtain tumor cells coated with coatings; the plant polyphenol and the manganese ions can be quickly assembled at room temperature, a compact nano coating is formed on a tumor cell membrane, and tumor cells are inactivated due to the wrapping of the nano coating, so that the safety of the vaccine is ensured; the nano coating can prevent any potential tumor antigen from being lost under physiological conditions; lipopolysaccharide is further modified, so that the formed LMP whole-cell vaccine can be promoted to be endocytosed by antigen presenting cells; the metal manganese ions can stimulate the STING pathway to enhance the anti-tumor effect while forming the structural coating.

The invention discloses an alveolar macrophage glycyrrhetinic acid liposome and a preparation method thereof, and belongs to the field of biological medicines. The surface of glycyrrhetinic acid lipidosome is modified with alveolar macrophage targeting molecules, so that the lipidosome can specifically target M1 alveolar macrophages induced by lipopolysaccharide serving as a main component of the outer wall of gram-negative bacteria, entrapped glycyrrhetinic acid is ingested by the alveolar macrophages, M2 polarization is specifically induced, and the specific targeting effect is achieved. Lung inflammatory injury caused by gram-negative bacteria is reduced, lung tissue injury and fibrosis caused by excessive inflammation are prevented, and great significance is achieved for pneumonia caused by gram-negative bacteria.

The invention belongs to the field of biological medicine, particularly discloses application of glycyrrhetinic acid or glycyrrhetinic acid lipidosome in preparation of an abdominal aortic aneurysm protection medicine, and reveals that glycyrrhetinic acid can effectively improve death of mice after aorta rupture caused by Ang II treatment, can obviously improve degradation of an aorta elastic fiber layer caused by Ang II treatment, and can improve the aortic aneurysm protection effect. The macrophages induced by lipopolysaccharide (LPS) can be promoted to be polarized to M2 type; the glycyrrhetinic acid is used for intervention before Ang II treatment, the occurrence of Ang II induced abdominal aortic aneurysm can be relieved, the survival rate of mice is increased, and it is indicated that the glycyrrhetinic acid can be used for preparing the medicine for relieving the abdominal aortic aneurysm. Furthermore, the glycyrrhetinic acid is entrapped into the liposome, so that the solubility and bioavailability of the glycyrrhetinic acid are improved, meanwhile, after glycyrrhetinic acid coating is carried out by utilizing a nanotechnology, the dosage of the glycyrrhetinic acid can be effectively controlled, and the curative effect of the glycyrrhetinic acid is further improved and enhanced.

The present invention discloses the preparation of an anti-inflammatory peptide derived from the hydrolysis of shell nacre protein and its application in skin repair. The peptide PDFDNGF provided by the invention can effectively promote the proliferation of RAW264.7 macrophage cells and inhibit the excessive production of nitric oxide and cytokines in RAW264.7 cells induced by lipopolysaccharide, while increasing the levels of anti-inflammatory cytokines, demonstrating significant anti-inflammatory activity. Furthermore, the active peptide has a significant proliferative effect on L929 cells, indicating its potential to promote skin wound healing. This invention provides a theoretical basis for the high-value utilization of shell nacre.

The invention discloses a preparation method and antipyretic and anti-inflammatory application of a novel carrier-free metal-organic small molecule supramolecular hydrogel derived from Maxing Shigan Decoction. The hydrogel drug raw materials of the invention include pseudoephedrine hydrochloride in Ephedra sinica in the prescription of Maxing Shigan Decoction, amygdalin in Armeniaca vulgaris, magnesium ions in Gypsum fibrosum, and glycyrrhizic acid in Glycyrrhiza uralensis Fisch. The preparation method of the quaternary magnesium hydrogel drug is simple, green, has good stability and high biocompatibility. In a rat fever model induced by lipopolysaccharide at different doses, the antipyretic effect of the hydrogel drug can last up to 12 hours, and the antipyretic effect is significantly better than that of other metal ions such as quaternary calcium hydrogel, quaternary zinc hydrogel, and quaternary copper hydrogel groups. At the same time, it has a more persistent antipyretic effect than the whole prescription of Maxing Shigan Decoction; the hydrogel drug does not require the addition of excipients, has no toxic and side effects, and has a high drug utilization rate.

The present invention relates to an ELISA antibody detection method for serotype 2 Riemerella anatipestifer and an application thereof. The method is based on a bacterial lipopolysaccharide coated plate, and an indirect ELISA method for serotype 2 Riemerella anatipestifer is established through optimization of optimal reaction conditions, determination of critical values, specificity tests, sensitivity tests, repeatability tests and clinical trials. Compared with the prior art, the present invention extracts serotype 2 Riemerella anatipestifer lipopolysaccharide by hot phenol water method and coats the ELISA plate. By optimizing reaction conditions such as antigen coating conditions, dilution multiple and incubation conditions of serum to be tested / rabbit anti-duck enzyme-labeled antibodies, and substrate color development time, an indirect ELISA method for detecting serotype 2 Riemerella anatipestifer antibodies is successfully established.

The invention relates to a biomarker for screening intestinal flora transplantation donors and application of the biomarker. The biomarker comprises intestinal flora and metabolites thereof, the intestinal flora comprises phylum firmicalis, bacteroides and the like, and the metabolites comprise short-chain fatty acid, tryptophan, indole, 5-hydroxytryptamine, lipopolysaccharide and the like. According to the method, the intestinal flora of different crowds is deeply analyzed, the relationship between each related index of the intestinal flora and the intestinal flora transplantation effect is studied, sufficient clinical verification is carried out, a biomarker for screening the intestinal flora transplantation donor is excavated, and a standard for judging whether the intestinal flora transplantation donor is qualified or not is established; and a new method and a new idea are provided for efficiently and accurately screening intestinal flora transplantation donors.

A Bifidobacterium longum subsp. longum dipro-017. The Bifidobacterium longum subsp. longum dipro-017 used has the ability to metabolize tryptophan to produce indole-3-lactic acid, can inhibit the pathogenic bacteria Salmonella Typhimurium, Staphylococcus aureus, and Escherichia coli, and can effectively mitigate weight loss caused by ulcerative colitis, improve a DAI score, improve colon length, mitigate the content of lipopolysaccharide (LPS) in blood, and increase the level of anti-inflammatory cytokine IL-10, as well as mitigate the content of bone metabolism indicators tartrate-resistant acid phosphatase (TRAP) and N-terminal propeptide of type I procollagen (PINP) in blood.

The invention discloses a minimally invasive flexible piezoelectric-triboelectric hybrid nanometer nerve stimulation device and application thereof.The middle layer of the nerve stimulation device is a PVDF / TMCM-MnCl3 composite nanofiber membrane, Au electrodes are evaporated on the two faces of the PVDF / TMCM-MnCl3 composite nanofiber membrane, two gold wire or copper wire electrodes are connected with the Au electrodes respectively, and the two gold wire or copper wire electrodes are connected with the Au electrodes respectively. The surface of the Au electrode is subjected to primary packaging by adopting a PU waterproof film, and the surface of the PU waterproof film is subjected to secondary packaging by adopting PDMS. The nerve stimulation device is used for conventional treatment of SIRS. The vagus nerve stimulator can generate an adjustable current up to 0.85 mA under programmable ultrasonic driving, and can be directly used for VNS. The PTNG is ultrasonically driven in vitro to assist the VNS in treating lipopolysaccharide-induced SIRS rats, release of inflammatory factors such as TNF-alpha, IFN-gamma and IL-1beta can be effectively reduced, cytokine storm is relieved, and multi-organ tissue damage and dysfunction are relieved, so that the survival hope is improved.

The invention belongs to the technical field of biological medicines, and particularly discloses an inflammatory memory activated epidermal stem cell bionic vesicle as well as a preparation method and application thereof. The epidermal stem cell bionic vesicle is obtained by adding lipopolysaccharide into a serum-free culture medium to induce and culture epidermal stem cells, continuously extruding a cell suspension through an extruder of a micron-sized polycarbonate film, and carrying out centrifugal separation. The LEM preparation method provided by the invention is simple, efficient and good in biocompatibility, not only retains the repair characteristic of mother cells, but also can induce neutrophil to transform to N2 phenotype, and has a better inflammation regulation effect, so that wound healing and tissue repair are promoted, and the LEM has a better application prospect and a better application value.

The invention discloses application of sodium butyrate to prevention and treatment of dairy cow mastitis, and belongs to the technical field of biology. The invention can provide an application of sodium butyrate in preventing and treating cow mastitis, in particular an application in preparing a preparation for preventing and treating cow mastitis induced by lipopolysaccharide (LPS) in escherichia coli. According to the application of the sodium butyrate in the aspect of preventing and treating the dairy cow mastitis, expression of key factors such as mammary gland tight junction protein is up-regulated under stimulation of lipopolysaccharide (LPS), and the sodium butyrate can effectively inhibit apoptosis and enhance the barrier function of mammary gland acinus, so that the effect of preventing and treating the dairy cow mastitis is achieved.

The invention discloses application of plant-derived glyceroglycolipid in preparation of an anti-inflammatory product, inflammation is RAW264.7 cell inflammatory response induced by LPS (lipopolysaccharide), and the plant-derived glyceroglycolipid can resist inflammation by inhibiting expression of inflammatory factors. By combining column chromatography with chromatographic analysis, efficient extraction, precise separation and structural identification of the plant GL are realized, and important technical support is provided for research of plant lipids. The plant GL can significantly inhibit inflammatory response of RAW264.7 macrophages, including reduction of LPS-induced cellular morphological change, down-regulation of inflammatory factor mRNA expression and reduction of CD86 expression level. The invention provides a solid molecular level theoretical basis for the application of the plant glycerol glycolipid in the development of anti-inflammatory functional foods and medicines.

The invention provides an extraction and detection method of a day lily active matter and application of the day lily active matter in anti-neuritis. Hemerocallis fulva contains various bioactive substances including flavone, polysaccharide and saponin, and the substances have potential application value in the fields of medicines and health care products. The invention provides a method for extracting the active components step by step, and rapid identification and detection are carried out by utilizing technologies such as fluorescence and ultraviolet-visible spectrophotometry. In addition, the invention also develops a detection method for evaluating the anti-neuritis effect of the active substances. Lipopolysaccharide (LPS) is used for stimulation to establish an inflammation model, day lily active substances are added, and the active substances are found to be capable of remarkably inhibiting generation and release of inflammatory factors and promoting growth and differentiation of neurons. Scientific basis is provided for developing new neuritis treatment methods, related drugs and the like.

The invention belongs to the field of microbial culture, and particularly relates to an enrichment medium for reducing expression of surface polysaccharide of vibrio parahaemolyticus. According to the formula of the polysaccharide low-expression enrichment culture medium, nutritional ingredients in a specific proportion and specific polysaccharide expression inhibition conditions are selected, so that the expression quantity of surface lipopolysaccharide and capsular polysaccharide after enrichment of vibrio parahaemolyticus is lower than the expression quantity of polysaccharide after enrichment of normally cultured vibrio parahaemolyticus; expression of lipopolysaccharide and capsular polysaccharide on the surface layer can be inhibited to a certain extent, so that outer membrane protein can be better exposed on the surface of a thallus, and immunodetection of vibrio parahaemolyticus is facilitated. The invention provides a technical basis for developing a complete vibrio parahaemolyticus immunodetection method.

The invention discloses a strain of lactobacillus reuteri YSLR01 and application of the strain of lactobacillus reuteri YSLR01 in preparation of anti-inflammatory, anti-oxidation, fat-reducing and anti-aging products. According to the invention, a YSLR01 strain with anti-inflammatory, anti-oxidation, fat-reducing and anti-aging effects is separated from excrement of healthy human beings, the strain is preserved in China General Microbiological Culture Collection Center on September 2, 2024, and the preservation number is CGMCC NO.31800. The invention further discloses a preparation method of the YSLR01 strain. Researches show that the YSLR01 strain can significantly reduce secretion of inflammatory factors in a lipopolysaccharide (LPS)-induced RAW264.7 cell inflammation model, improve the oxidation resistance of caenorhabditis elegans, enhance the body length, action ability and reproductive capacity of the caenorhabditis elegans, reduce fat deposition in the body and delay aging of the body.

The invention belongs to the field of antidepressant drugs, and discloses an antidepressant composition containing compound liquorice tablets and application of the antidepressant composition. The composition obtained by compounding the compound licorice tablets and various traditional Chinese medicine extracts has the effects of soothing liver-qi stagnation, calming heart and tranquilizing mind, nourishing blood and regulating liver, and treating qi and blood at the same time, can significantly improve depressive symptoms caused by LPS stimulation, and has a good anti-depression effect. On the basis, a fingerprint control mode based on the dual-wavelength high performance liquid chromatography standard characteristics of the compound liquorice tablets is established. According to the method, macro qualitative similarity and macro quantitative similarity are used for checking the change range of the overall component content and the quality consistency control condition of the compound liquorice tablets. The quality consistency of the compound liquorice tablets is finally not less than 0.95 in terms of macro qualitative similarity of 220nm and 260nm high performance liquid chromatography fingerprints, the macro quantitative similarity is between 85% and 115%, and the compound liquorice tablets have the effect of improving the depression behavior of mice caused by LPS (Lipopolysaccharide).

The invention provides an anti-inflammatory substance and application thereof, and relates to the technical field of molecular biology. The docosahexaenoic acid can be used for effectively inhibiting expression of mRNA (messenger ribonucleic acid), protein and supernatant of IL-1beta (interleukin-1beta) of RAW264.7 cells induced by LPS (lipopolysaccharide); in addition, the docosahexaenoic acid can play a role in relieving inflammation in a transdermal administration mode, and the technical effect of inhibiting or treating inflammation in a non-invasive and safe mode is achieved.

The invention relates to an isopentenyl-alpha-pyrone dimer Hyperumonates D-F as well as a preparation method and application thereof, and belongs to the field of medical chemistry, the isopentenyl-alpha-pyrone dimer Hyperumonates D-F is obtained by extracting and separating from leaves and branches of Hypericum monogynum for the first time, and the structural formula of the isopentenyl-alpha-pyrone dimer Hyperumonates D-F is D-F. The invention further discloses a preparation method of the isopentenyl-alpha-pyrone dimer Hyperumonates D-F. The isopentenyl-alpha-pyrone dimer Hypericumonates D-F has the advantages that the compounds D-E have remarkable inhibition effects on generation of NO in mouse microglial cells (BV-2 cells) induced by lipopolysaccharide (LPS), the activity of the compounds D-E is higher than that of positive medicine minocycline, and the compounds D-E have application potentials in the field of neuroinflammation medicines, and the invention also relates to a pharmaceutical composition which comprises the isopentenyl-alpha-pyrone dimer Hypericumonates D-F and can be used for preparing medicines for treating neuroinflammation. And pharmaceutically acceptable auxiliary materials, wherein the pharmaceutical composition has the effect of resisting neuroinflammation.

The invention relates to application of visceral odorobacter and outer membrane vesicles thereof in preparation of drugs for treating acute lung injury, and relates to the technical field of drugs. The modeling medicine lipopolysaccharide is applied to a mouse individual in a nasal dripping mode, the administration mode is that each mouse is administered once a day, and intragastric administration is carried out once a day after modeling is carried out for two days and lasts for 7 days. Experiments prove that visceral odorobacter and outer membrane vesicles of the visceral odorobacter reduce the number of M1 type alveolar macrophages by down-regulating proinflammatory factors IL-6, TNF-alpha and IL-1beta in lung tissues and inhibiting three proinflammatory factors IL-6, TNF-alpha and IL-1beta in serum, so that the effect of remarkably reversing acute lung injury is achieved. The visceral odor bacilli and the outer membrane vesicles thereof are used for preventing and treating the acute lung injury, and a new method and means are provided for clinically treating the acute lung injury.

The invention discloses a crocodile polypeptide with anti-inflammatory and intestinal flora regulating functions and a preparation method thereof, belongs to the technical field of functional polypeptide preparation, and particularly relates to a preparation method of the crocodile polypeptide, an anti-inflammatory effect of the crocodile polypeptide and a regulating effect of the crocodile polypeptide on intestinal flora in ulcerative colitis inflammation. According to the preparation method, the crocodile polypeptide is prepared by combining multiple physical processing with enzymolysis, so that the purity and the biological activity of the crocodile polypeptide are improved. On the basis, a lipopolysaccharide-induced THP-1 in-vitro cell inflammation model and a dextran sodium sulfate-induced mouse in-vivo ulcerative colitis inflammation model are established, and the in-vivo and in-vitro anti-inflammatory effect of the crocodile polypeptide is further researched. The crocodile polypeptide prepared by the invention has a remarkable inhibition effect on THP-1 cell inflammation induced by lipopolysaccharide. Meanwhile, the crocodile polypeptide prepared by the preparation method disclosed by the invention has an adjusting effect on intestinal flora. The food or functional food with the effects of relieving ulcerative colitis and regulating intestinal flora can be prepared.

The invention discloses a construction and evaluation method of a PM2.5 induced chronic obstructive pulmonary disease acute exacerbation mouse model, and relates to the technical field of disease animal models. The invention aims to evaluate whether PM2.5 can induce acute exacerbation of COPD through a mouse model, a COPD model is established by adopting combined treatment of smoking and lipopolysaccharide (LPS), and an infectious AECOPD model is established by using common klebsiella pneumoniae (KP) nasal drop on the basis. Meanwhile, PM2.5 processing is added on the basis of the COPD model, and the effect of PM2.5 on acute exacerbation of COPD is evaluated. Through comparison with a classical model (an infectious AECOPD model), the potential effect of PM2.5 in induction of acute exacerbation of COPD is evaluated, and an important reference is provided for future research on an AECOPD animal model induced by air pollution.

The invention discloses an Akkermansiuciniphila rough type lipopolysaccharide with an effect of preventing and treating diseases and an application of the Akkermansiuciniphila rough type lipopolysaccharide. The lipopolysaccharide has the effect of preventing / treating diseases, and the diseases include but are not limited to prevention / treatment of metabolic syndrome, atherosclerosis and intestinal infection.

The invention belongs to the technical field of natural pharmaceutical chemistry, and discloses a benzofuran compound in dalbergia odorifera and a preparation method and application thereof.The benzofuran compound is separated from dalbergia odorifera of a leguminous dalbergia plant, the heartwood of the dalbergia odorifera is extracted and concentrated to obtain extract, and the extract is filtered to obtain the benzofuran compound. The extract is taken and sequentially subjected to silica gel column chromatography, medium-pressure ODS column chromatography and gel column chromatography separation for multiple times, then semi-preparative high performance liquid chromatography is used for purification, and the benzofuran compounds can be obtained. According to the present invention, lipopolysaccharide (LPS) is adopted to induce RAW 264.7 cells to establish an inflammation model, such that the compound has good anti-inflammatory activity, IC50 is 14.33 [mu] M, and expression of inflammatory factor interleukin-6 (IL-6) and interleukin-1 [beta] (IL-1 [beta]) can be inhibited;

The use of 2'-fucosyllactose in the preparation of a food / drug for promoting intestinal glycocalyx development and preventing intestinal glycocalyx damage caused by lipopolysaccharide (LPS). The use of 2'-fucosyllactose in the preparation of a food / drug for the adjuvant treatment and / or adjuvant prevention of gastrointestinal infections, inflammatory bowel diseases, obesity or allergy caused by glycocalyx damage. Before adherent growth of Caco-2 cells, adding 2'-FL for co-culture with the Caco-2 cells can simulate the intestinal growth and maturation state. In the state, 2'-FL can significantly promote the development of the glycocalyx layer of Caco-2 cells, and can further prevent the glycocalyx layer damage caused by LPS on this basis.