175results about "Oligosaccharides" patented technology

The application provides a Huangdache oligosaccharide with GLP-1 agonist activity and application thereof, and belongs to the technical field of medicines. 3 The oligosaccharide component ELYP-3 has a molecular weight of 3.2*10 3 Da, and the molar ratio of monosaccharides is rhamnose:galacturonic acid:glucose:galactose:arabinose = 1:32.42:45.37:9.11:6.54. The Huangdache oligosaccharide fragment obtained by glycosidase degradation has significant hypoglycemic and weight loss activities, the preparation process is simple, specific and green, and provides scientific basis and theoretical support for the development of natural GLP-1 agonists. Meanwhile, the functional oligosaccharide provides a wide application prospect for the development of medicines with blood glucose regulation.

The preparation method comprises the following steps: (1) dissolving agar in a citric acid monohydrate solution, and carrying out acidolysis to obtain an acidolysis solution; centrifuging, filtering, adding beta-agarase AgWH50B, and carrying out enzymolysis for 20-28 hours at the temperature of 30-35 DEG C; boiling, centrifuging and filtering to obtain supernate, namely crude oligosaccharide liquid containing agarotriose; (2) dissolving agar in a phosphoric acid solution, and degrading for 55-65 minutes at the temperature of 97-103 DEG C; centrifuging and filtering to obtain supernate, namely crude oligosaccharide liquid containing agarobiose; (3) dissolving agar in deionized water, adding alpha-agarase A33, and carrying out enzymolysis at 38-42 DEG C for 20-28 hours; and boiling, centrifuging and filtering to obtain supernate, namely crude oligosaccharide liquid containing agarotetraose. The preparation method of the agaro-oligosaccharide is used for preparing agarotriose, agarobiose and agarotetraose, and the product purity is high. The method can provide guidance for subsequent large-scale industrial production of agaro-oligosaccharide.

The application discloses a high-precision intelligent control and adjustment method for stachydrine fermentation and purification, and relates to the field of biological fermentation process control. The method comprises the following steps: obtaining a stachydrine mixed solution in a first sugar content interval; inoculating Eurotium cristatum at a first preset temperature after sterilization to start fermentation and obtain an initial fermentation liquor; monitoring the content ratio change of stachydrine and non-target sugars in real time through HPLC to obtain sugar content monitoring data; introducing a membrane separation device for selective dialysis to obtain membrane permeation rate monitoring data; performing dynamic evaluation on stachydrine enrichment according to the sugar content monitoring data and the membrane permeation rate monitoring data, generating a dynamic adjustment strategy for fermentation conditions based on the evaluation results; and performing fermentation process adjustment control. The method solves the technical problem that the existing stachydrine fermentation and purification cannot realize real-time and accurate monitoring and dynamic adjustment of fermentation conditions, resulting in low fermentation efficiency and low purity, and achieves the technical effect of improving the fermentation efficiency and purity of stachydrine through intelligent control of the fermentation process.

The invention discloses a method for extracting and preparing an oligosaccharide extract from codonopsis pilosula and application of the oligosaccharide extract in prevention and treatment of neurodegenerative disease Alzheimer's disease, and belongs to the technical field of traditional Chinese medicine pharmacy. According to the present invention, the oligosaccharide extract is enriched and prepared from Codonopsis pilosala (Franch.) Nanf., wherein the Codonopsis pilosala (Franch.) Nanf. Is a plant of the family of Platycodon grandiflorum, and the activity test of the oligosaccharide extract on the improvement of cognitive dysfunction in the animal body is performed; results show that in a mouse cognitive function impairment model caused by sleep deprivation, the high-dose codonopsis pilosula oligosaccharide has a prominent effect on improving the learning and memory ability of a Morris water maze and a neuroprotective effect, the efficacy of the codonopsis pilosula oligosaccharide is equivalent to that of a positive control drug manntranet, and no obvious toxic or side effect is found. The codonopsis pilosula oligosaccharide is expected to be further developed into functional food, health care products and / or medicines with the effect of preventing and / or improving cognitive impairment.

The application discloses a method for extracting soybean saponins and soybean oligosaccharides, and belongs to the technical field of natural product extraction. The method comprises the following steps: puffing treatment of soybean meal, wall breaking treatment in a high-voltage electric field; acid precipitation, collection of supernatant; after ultrasonic oscillation of the supernatant, a composite enzyme is added for enzymolysis, and a material liquid after enzymolysis is obtained; after filtration of the material liquid, the material liquid is loaded onto a macroporous resin column for adsorption, and after completion of the adsorption, effluent is collected; the effluent is purified and concentrated to obtain soybean oligosaccharides; an ethanol solution is used to elute the resin column, and eluate is collected; the eluate is recovered by ethanol and dried to obtain soybean saponins; the composite enzyme comprises amylase and beta-glucosidase. The method can significantly improve the extraction rate and purity of soybean saponins and soybean oligosaccharides, the whole process uses water as the main solvent, the consumption of organic solvents and the pollution to the environment are reduced, and the unit process water consumption, unit energy consumption and unit cost are reduced.

This invention discloses an acid-catalyzed controlled degradation and recycling method for waste polyester and polyester-cotton blended fabrics. Using waste polyester or waste polyester-cotton blended fabrics as raw materials, under the action of an acidic catalyst, in a mixed solvent composed of a polar aprotic solvent and water, the method involves heating and degradation, causing the polyester in the waste polyester or polyester-cotton blended fabrics to depolymerize or co-depolymerize, generating organic raw materials. This invention achieves complete depolymerization and recycling of polyester with a relatively low acid dosage, effectively avoiding the corrosion problems of equipment and excessive degradation of cotton fibers caused by existing technologies. It is also more conducive to the generation and recovery of glucose and 5-hydroxymethylfurfural. Furthermore, the separation steps are simple, greatly reducing separation costs, and high-yield recovery of terephthalic acid, glucose, and 5-hydroxymethylfurfural is achieved, realizing high-value recycling and utilization of various components in textiles, reducing waste, and improving resource utilization.

The present disclosure discloses the identification and introduction of a specific heterologous gene encoding an alpha-1,2-fucosyltransferase (denoted smob) into LNT producing strains for the specific production of LNFP-I. The smob gene is derived from the microorganism Sulfuriflexus mobilis (https: / / www.dsmz.de / collection / catalogue / details / culture / DSM-102939), a sulfur-oxidizing bacterium isolated from a saline lake sediment.

The present disclosure relates to the production of complex fucosylated Human Milk Oligosaccharides (HMOs) and in particular to the production of complex fucosylated HMOs with five or more monosaccharide units, such as LNFP-III, LNFP-VI and LNDFH-III as well as mixtures thereof. The present disclosure also relates to genetically engineered cells and α-1,3-fucosyltransferases suitable for use in said production, as well as to methods for producing said fucosylated HMOs.

The present invention provides a new antibacterial compound named nidaramycin, which is derived from a novel biosynthetic gene cluster (BGC), and its use. In addition, this specification provides novel genes and nucleic acid molecules encoding the biosynthetic machinery for the production of nidaramycin, as well as constructs, vectors, and host cells for expressing the BGC, and methods for producing the compound.

The invention belongs to the technical field of biomass conversion and functional food ingredients, and discloses a method for preparing galactooligosaccharide prebiotics from cellulose. The method comprises the following steps: mixing cellulose with a galactose aqueous solution, and drying to obtain galactose pre-impregnated cellulose; impregnating the cellulose pre-impregnated with galactose with an acid catalyst solution, and drying to obtain cellulose impregnated with galactose and an acid catalyst; carrying out solvent-free ball milling treatment on the cellulose impregnated with the galactose and the acid catalyst, so that the cellulose is synchronously subjected to depolymerization and grafting reaction with the galactose to obtain a crude product; and carrying out purification treatment on the crude product, and removing the residual acid catalyst to obtain the galactooligosaccharide prebiotics. According to the galactooligosaccharide prepared by the preparation method disclosed by the invention, the water solubility of the oligosaccharide is greatly improved due to the introduction of a galactose branched chain, the yield of a final product is high, and the prebiotic activity of the final product is equivalent to that of a high-cost enzymic method commercial product and even is better for a specific strain.

Provided herein are methods for preparing a product including predominantly prebiotic oligosaccharides by non-enzymatic methods of glycosylation of monosaccharides, disaccharides, and / or polysaccharides. The methods may include mixing one or more types of monosaccharides, disaccharides, and / or polysaccharides with an effective amount of a dehydrating acid at a temperature sufficient to form a product including predominantly prebiotic oligosaccharides.

The application provides a yeast mannose oligosaccharide and a preparation method and application thereof. The preparation method comprises the following steps: S1, mixing yeast cell walls with water to obtain a mixed solution; S2, adjusting the pH of the mixed solution to 4-9, performing first enzymolysis on the mixed solution by using a protease to form a first enzymolysis product; secondly, performing second enzymolysis on the first enzymolysis product by using a polysaccharide enzyme to obtain a second enzymolysis product; the amount of the protease is greater than or equal to 0.2% of the weight of the yeast cell walls, and the amount of the polysaccharide enzyme is greater than or equal to 0.1% of the weight of the yeast cell walls; S3, centrifuging the second enzymolysis product to obtain a supernatant and a centrifugal heavy phase; filtering the supernatant by using an ultrafiltration membrane, and the retentate is the yeast mannose oligosaccharide, the permeate is a yeast extract, and the centrifugal heavy phase is a yeast glucan. The yeast mannose oligosaccharide obtained by the application has high purity and high yield, the mannose oligosaccharide is clean, can be fermented by beneficial bacteria, and can be applied as a prebiotic in food.

Provided is a method for producing an agarooligosaccharide-containing composition, which can improve yield and productivity. The method for producing an agarooligosaccharide-containing composition according to the present invention comprises an extraction step of immersing red algae in water at 70 to 120°C to extract a red algae extract, and a decomposition step of hydrolyzing polysaccharides contained in the extracted red algae extract, wherein the decomposition step is carried out to obtain a dispersion of the red algae extract having a kinematic viscosity of 5,000 to 300,000 mm at a product temperature of 70°C. 2 The method is characterized in that the polysaccharide is hydrolyzed by exposure to an acid with an adjusted concentration so that the concentration is in the range of 1 / s.

The present invention provides a method of treating constipation and compositions useful in said method. The method comprises administering to a subject in need thereof an effective amount of a crosslinked carboxymethylcellulose having high elastic modulus coupled with high absorbance capacity when swollen in simulated gastric fluid / water (1:8) and simulated intestinal fluids.

An object is to provide a method for effectively utilizing byproducts of the production of dairy products. Another object is to provide a method for producing galactosylkojibiose having a superior prebiotic ability. Another object is to provide a solution or composition comprising galactosylkojibiose, wherein at least 80% of galactosylkojibiose is maintained even after heated. A method for producing a composition comprising an oligosaccharide, the method comprising the step of allowing a glucansucrase to act on a composition comprising lactose and sucrose to obtain a composition comprising galactosylkojibiose is provided. It is preferable to use a lactic acid bacterium that constitutively expresses a membrane-bound type glucansucrase. There are provided a solution containing galactosylkojibiose, which has a pH lower than 7, and a composition comprising galactosylkojibiose, wherein when the composition is made into a solution having a solid content concentration of 10 to 80%, the solution has a pH lower than 7.

This invention relates to a catalyst for degrading polysaccharides, its preparation method, and its application, belonging to the field of polysaccharide degradation technology. The invention provides a method for preparing a catalyst for degrading polysaccharides, comprising: weighing fumaric acid and hydrogen peroxide, mixing them, and preparing the catalyst. The molar ratio of fumaric acid to hydrogen peroxide is 0.5–2:0.5–2. The invention also provides a catalyst for degrading polysaccharides, obtained by the above preparation method. The catalyst prepared by this invention using fumaric acid and hydrogen peroxide for the degradation of galactomannan can improve the polysaccharide degradation efficiency, resulting in high yields of low molecular weight galactomannan and galactomannan oligosaccharides, thus increasing productivity.

The invention discloses a preparation technology of astragalus polysaccharide camouflage monosaccharide, and relates to the technical field of biological product production, the preparation technology comprises the following steps: S1, extraction, S2, purification: carrying out purification treatment on astragalus polysaccharide extracted in the step 1, S3, degradation camouflage: dissolving the astragalus polysaccharide purified in the step 2 in a solvent, carrying out degradation treatment on the astragalus polysaccharide by adopting ultrasonic waves, and carrying out purification treatment on the astragalus polysaccharide to obtain the astragalus polysaccharide camouflage monosaccharide. A specific glucan structural unit in a polysaccharide chain is degraded and disguised into a monosaccharide or oligosaccharide structure; and S4, separating to obtain a product. Astragalus polysaccharide is directionally camouflaged as a monosaccharide / oligosaccharide structure, the recognition and uptake efficiency of astragalus polysaccharide by cancer cells is remarkably enhanced, so that the targeting of inducing apoptosis of cancer cells is improved, the structure can effectively activate and train immune cells such as dendritic cells and strengthen anti-tumor immune response, and meanwhile, the product can interfere with a metabolic pathway of cancer cells and reverse the drug resistance of cancer cells, so that the anti-tumor effect is improved. Nutrition supply is blocked to inhibit tumor growth, the production process is green and controllable, and good clinical transformation and industrialization prospects are achieved.

The invention discloses a preparation method of low-moisture and low-water-activity xylooligosaccharide powder, and belongs to the field of functional sugar alcohol. The method comprises the following steps: taking a xylooligosaccharide solution with the refractive index of 35-50%, adding activated carbon for decoloration, filtering, adding deionized water to adjust the refractive index to 40-48%, and purifying through cation exchange resin, anion exchange resin, anion exchange resin and cation exchange resin in sequence; evaporating the purified solution until the refractive index is 50-55%; homogenizing the concentrated solution, spraying the homogenized concentrated solution into a spray drying tower through a spray head, and drying the homogenized concentrated solution in a heating evaporation area and a cooling collection area under the pressure of 100-150Pa in the tower; and drying, screening with a 60-mesh screen, feeding into a clean room, cooling, and packaging. The water content of the prepared xylooligosaccharide powder is 0.1%-1%, the water activity is 0.1% or below, the product fluidity is good, crushing treatment is not needed, the process is simple and efficient, and the xylooligosaccharide powder is suitable for industrial production.

The invention discloses a high-efficiency separation method of breast milk oligosaccharide and application of the breast milk oligosaccharide in specific recognition of influenza A virus hemagglutinin protein.The method comprises the steps that a DE-52 weak anion exchange column, a graphite carbon column and Bio-GelP10 column chromatography are combined for use, breast milk oligosaccharide components with low abundance and high molecular weight are efficiently enriched, and the specific recognition of influenza A virus hemagglutinin protein is achieved; after derivatization labeling of a bifunctional reagent 2-amino-N-(2-aminoethyl) benzamide (AEAB), 56 breast milk oligosaccharide monomers with the purity higher than 90% are separated from the isomer level through one-dimensional amide normal phase chromatography and two-dimensional C18 reverse phase circulation chromatography, and a monomer substance library is constructed. AEAB is used as a connecting arm to construct a breast milk oligosaccharide chip, an active monomer DSLNT II which is specifically combined with four subtype influenza A virus hemagglutinin proteins is screened out in a high-throughput manner, and fine structure analysis is completed in combination with electrospray tandem mass spectrometry. According to the method, the large-scale preparation of the low-abundance breast milk oligosaccharide monomer is realized, and a key material basis and theoretical guidance are provided for researching an antiviral mechanism and developing related functional foods and medicines.

Mutated lacto-N-biosidases are provided with improved properties compared to the wild type lacto-N-biosidase isolated from Bifidobacterium longum.

The present invention relates to the use of a cosmetic active ingredient comprising an extract of the biomass of at least one yeast of the species Aureobasidium pullulans, characterized in that the extract contains at least 25% by weight of sugars based on the dry matter of the extract, as a cosmetic active ingredient in a use or composition comprising the same.

The invention relates to a method for the separation of two hydrophilic neutral oligosaccharides from each other with a chromatography on a bromine functionalized polystyrene cross-linked with divinylbenzene (BPS-DVB) stationary medium.

A method for increasing the value of a polysaccharide containing material obtained from a fruit that is not a pomegranate by generating a mixture of polysaccharide cleavage products. The method comprises (i) reacting a fruit-based starting material containing polysaccharide with a Fenton's reagent containing a peroxide agent and a metal, and (ii) cleaving the reacted polysaccharide with a cleavage agent to generate the mixture of polysaccharide cleavage products. The polysaccharide cleavage products generally contain higher amounts of oligosaccharides, lower average molecular weight, increased solubility, and decreased viscosity when dissolved in aqueous solution compared to the fruit-based starting material. Also provided are compositions and soluble fruit fiber comprising mixtures of polysaccharide cleavage products.

The application discloses a preparation method of codlane oligosaccharide, and comprises the following steps: mixing codlane polysaccharide with L molecular sieve, ball-milling and grinding, adding a solvent to dissolve, separating the L molecular sieve from the solid-liquid, and drying to obtain the codlane oligosaccharide; the L molecular sieve has a chemical composition of 1-5K2O*1.0Al2O3*5-10SiO2*1-5H2O and has straight pores; the L molecular sieve with the straight pores can selectively cut the glycosidic bond of the codlane to prepare the codlane oligosaccharide; thus, the codlane oligosaccharide can be efficiently prepared, and the L molecular sieve can be repeatedly used; the method does not need a large amount of water or other solvents to participate in the reaction, has the advantages of environmental friendliness and low cost, and has obvious advantages compared with the prior art, and is more beneficial to industrialization.

The application discloses a preparation method of xylose, which comprises the following steps: S1, adding xylanase into a xylose mother liquor for enzyme hydrolysis and viscosity reduction; S2, adding calcium hydroxide into the enzyme hydrolysis liquor and passing in CO2 to generate nanometer calcium carbonate floccus to adsorb impurities; S3, performing cross-flow filtration on the ceramic-polyether sulfone composite membrane to improve the purity of the xylose; S4, performing multistage countercurrent chromatography separation on the calcium type strong acid cation resin, and dynamically adjusting an elution gradient through online HPLC feedback; and S5, performing three-effect evaporation concentration, then stage cooling crystallization, adding alpha type xylose crystal seeds to control the crystal form; the application utilizes enzyme hydrolysis-flocculation to cooperatively reduce the viscosity of the mother liquor to greater than or equal to 45%, and the nanometer floccus has an impurity adsorption efficiency greater than or equal to 85%; a dynamic chromatography separation factor is improved to 1.8, and the xylose recovery rate is greater than or equal to 96.2%; alpha type crystal purity greater than or equal to 99.5% is obtained through gradient crystallization, and the comprehensive energy consumption is reduced; and the enzyme hydrolysis conversion of the raffinate generates xylooligosaccharide and high fructose syrup.

The invention relates to the technical field of human body dietary nutrient dietary fibers. The average molecular weight of the apple gum is 50,000-250,000 Daltons, the average molecular weight of the apple gum can reach 160-50,000 Daltons after being subjected to low molecularization, the apple gum acts in human cells and can prevent toxic cation poisoning, and main methods for preparing oligosaccharide from pectin at present are divided into a physical method, a chemical method and a biological enzyme method. The method comprises the following steps: 1, processing apple peel residues into powder; the method comprises the following steps: heating and drying apple pomace, continuously taking 20-50% of the pomace, mixing the pomace with undried apple juice to 100% by weight, then drying by using a dryer, and repeating the processes of drying and mixing the pomace. And 2, carrying out secondary heating. And heating the powdered apple fibers at 120 DEG C for 30 minutes. And 3, spray drying. The powder is sprayed and dried at a certain rate through a spray dryer according to a ratio of about 3: 1 to about 2: 1, so that microstructures such as powder smell, color, roughness and the like are improved.

The invention discloses a marine polysaccharide degradation method and marine oligosaccharide, and relates to the technical field of marine polysaccharide degradation. The method comprises the following steps: S1, adding H2O2 into a 0.5%-2.5% (w / v) marine polysaccharide solution until the concentration of H2O2 in the solution reaches 1%-5% (w / v), and adjusting the pH value of the solution to 3-7 to obtain a first mixed solution; s2, performing microwave-ultrasonic treatment on the first mixed solution, and terminating the reaction after the microwave-ultrasonic treatment is completed to obtain a second mixed solution; s3, separating and purifying the second mixed solution, and freeze-drying to obtain the marine oligosaccharide. The method is easy and convenient to operate, green and environmentally friendly, the low-molecular-weight marine oligosaccharide can be efficiently prepared, and the prepared marine oligosaccharide is small in molecular weight and low in apparent viscosity.