98results about "Oligosaccharides" patented technology

The invention discloses a method for extracting and preparing an oligosaccharide extract from codonopsis pilosula and application of the oligosaccharide extract in prevention and treatment of neurodegenerative disease Alzheimer's disease, and belongs to the technical field of traditional Chinese medicine pharmacy. According to the present invention, the oligosaccharide extract is enriched and prepared from Codonopsis pilosala (Franch.) Nanf., wherein the Codonopsis pilosala (Franch.) Nanf. Is a plant of the family of Platycodon grandiflorum, and the activity test of the oligosaccharide extract on the improvement of cognitive dysfunction in the animal body is performed; results show that in a mouse cognitive function impairment model caused by sleep deprivation, the high-dose codonopsis pilosula oligosaccharide has a prominent effect on improving the learning and memory ability of a Morris water maze and a neuroprotective effect, the efficacy of the codonopsis pilosula oligosaccharide is equivalent to that of a positive control drug manntranet, and no obvious toxic or side effect is found. The codonopsis pilosula oligosaccharide is expected to be further developed into functional food, health care products and / or medicines with the effect of preventing and / or improving cognitive impairment.

The application discloses a method for extracting soybean saponins and soybean oligosaccharides, and belongs to the technical field of natural product extraction. The method comprises the following steps: puffing treatment of soybean meal, wall breaking treatment in a high-voltage electric field; acid precipitation, collection of supernatant; after ultrasonic oscillation of the supernatant, a composite enzyme is added for enzymolysis, and a material liquid after enzymolysis is obtained; after filtration of the material liquid, the material liquid is loaded onto a macroporous resin column for adsorption, and after completion of the adsorption, effluent is collected; the effluent is purified and concentrated to obtain soybean oligosaccharides; an ethanol solution is used to elute the resin column, and eluate is collected; the eluate is recovered by ethanol and dried to obtain soybean saponins; the composite enzyme comprises amylase and beta-glucosidase. The method can significantly improve the extraction rate and purity of soybean saponins and soybean oligosaccharides, the whole process uses water as the main solvent, the consumption of organic solvents and the pollution to the environment are reduced, and the unit process water consumption, unit energy consumption and unit cost are reduced.

This invention discloses an acid-catalyzed controlled degradation and recycling method for waste polyester and polyester-cotton blended fabrics. Using waste polyester or waste polyester-cotton blended fabrics as raw materials, under the action of an acidic catalyst, in a mixed solvent composed of a polar aprotic solvent and water, the method involves heating and degradation, causing the polyester in the waste polyester or polyester-cotton blended fabrics to depolymerize or co-depolymerize, generating organic raw materials. This invention achieves complete depolymerization and recycling of polyester with a relatively low acid dosage, effectively avoiding the corrosion problems of equipment and excessive degradation of cotton fibers caused by existing technologies. It is also more conducive to the generation and recovery of glucose and 5-hydroxymethylfurfural. Furthermore, the separation steps are simple, greatly reducing separation costs, and high-yield recovery of terephthalic acid, glucose, and 5-hydroxymethylfurfural is achieved, realizing high-value recycling and utilization of various components in textiles, reducing waste, and improving resource utilization.

The present invention provides a new antibacterial compound named nidaramycin, which is derived from a novel biosynthetic gene cluster (BGC), and its use. In addition, this specification provides novel genes and nucleic acid molecules encoding the biosynthetic machinery for the production of nidaramycin, as well as constructs, vectors, and host cells for expressing the BGC, and methods for producing the compound.

The invention belongs to the technical field of biomass conversion and functional food ingredients, and discloses a method for preparing galactooligosaccharide prebiotics from cellulose. The method comprises the following steps: mixing cellulose with a galactose aqueous solution, and drying to obtain galactose pre-impregnated cellulose; impregnating the cellulose pre-impregnated with galactose with an acid catalyst solution, and drying to obtain cellulose impregnated with galactose and an acid catalyst; carrying out solvent-free ball milling treatment on the cellulose impregnated with the galactose and the acid catalyst, so that the cellulose is synchronously subjected to depolymerization and grafting reaction with the galactose to obtain a crude product; and carrying out purification treatment on the crude product, and removing the residual acid catalyst to obtain the galactooligosaccharide prebiotics. According to the galactooligosaccharide prepared by the preparation method disclosed by the invention, the water solubility of the oligosaccharide is greatly improved due to the introduction of a galactose branched chain, the yield of a final product is high, and the prebiotic activity of the final product is equivalent to that of a high-cost enzymic method commercial product and even is better for a specific strain.

Provided herein are methods for preparing a product including predominantly prebiotic oligosaccharides by non-enzymatic methods of glycosylation of monosaccharides, disaccharides, and / or polysaccharides. The methods may include mixing one or more types of monosaccharides, disaccharides, and / or polysaccharides with an effective amount of a dehydrating acid at a temperature sufficient to form a product including predominantly prebiotic oligosaccharides.

The application provides a yeast mannose oligosaccharide and a preparation method and application thereof. The preparation method comprises the following steps: S1, mixing yeast cell walls with water to obtain a mixed solution; S2, adjusting the pH of the mixed solution to 4-9, performing first enzymolysis on the mixed solution by using a protease to form a first enzymolysis product; secondly, performing second enzymolysis on the first enzymolysis product by using a polysaccharide enzyme to obtain a second enzymolysis product; the amount of the protease is greater than or equal to 0.2% of the weight of the yeast cell walls, and the amount of the polysaccharide enzyme is greater than or equal to 0.1% of the weight of the yeast cell walls; S3, centrifuging the second enzymolysis product to obtain a supernatant and a centrifugal heavy phase; filtering the supernatant by using an ultrafiltration membrane, and the retentate is the yeast mannose oligosaccharide, the permeate is a yeast extract, and the centrifugal heavy phase is a yeast glucan. The yeast mannose oligosaccharide obtained by the application has high purity and high yield, the mannose oligosaccharide is clean, can be fermented by beneficial bacteria, and can be applied as a prebiotic in food.

The present invention provides a method of treating constipation and compositions useful in said method. The method comprises administering to a subject in need thereof an effective amount of a crosslinked carboxymethylcellulose having high elastic modulus coupled with high absorbance capacity when swollen in simulated gastric fluid / water (1:8) and simulated intestinal fluids.

An object is to provide a method for effectively utilizing byproducts of the production of dairy products. Another object is to provide a method for producing galactosylkojibiose having a superior prebiotic ability. Another object is to provide a solution or composition comprising galactosylkojibiose, wherein at least 80% of galactosylkojibiose is maintained even after heated. A method for producing a composition comprising an oligosaccharide, the method comprising the step of allowing a glucansucrase to act on a composition comprising lactose and sucrose to obtain a composition comprising galactosylkojibiose is provided. It is preferable to use a lactic acid bacterium that constitutively expresses a membrane-bound type glucansucrase. There are provided a solution containing galactosylkojibiose, which has a pH lower than 7, and a composition comprising galactosylkojibiose, wherein when the composition is made into a solution having a solid content concentration of 10 to 80%, the solution has a pH lower than 7.

This invention relates to a catalyst for degrading polysaccharides, its preparation method, and its application, belonging to the field of polysaccharide degradation technology. The invention provides a method for preparing a catalyst for degrading polysaccharides, comprising: weighing fumaric acid and hydrogen peroxide, mixing them, and preparing the catalyst. The molar ratio of fumaric acid to hydrogen peroxide is 0.5–2:0.5–2. The invention also provides a catalyst for degrading polysaccharides, obtained by the above preparation method. The catalyst prepared by this invention using fumaric acid and hydrogen peroxide for the degradation of galactomannan can improve the polysaccharide degradation efficiency, resulting in high yields of low molecular weight galactomannan and galactomannan oligosaccharides, thus increasing productivity.

The invention discloses a preparation technology of astragalus polysaccharide camouflage monosaccharide, and relates to the technical field of biological product production, the preparation technology comprises the following steps: S1, extraction, S2, purification: carrying out purification treatment on astragalus polysaccharide extracted in the step 1, S3, degradation camouflage: dissolving the astragalus polysaccharide purified in the step 2 in a solvent, carrying out degradation treatment on the astragalus polysaccharide by adopting ultrasonic waves, and carrying out purification treatment on the astragalus polysaccharide to obtain the astragalus polysaccharide camouflage monosaccharide. A specific glucan structural unit in a polysaccharide chain is degraded and disguised into a monosaccharide or oligosaccharide structure; and S4, separating to obtain a product. Astragalus polysaccharide is directionally camouflaged as a monosaccharide / oligosaccharide structure, the recognition and uptake efficiency of astragalus polysaccharide by cancer cells is remarkably enhanced, so that the targeting of inducing apoptosis of cancer cells is improved, the structure can effectively activate and train immune cells such as dendritic cells and strengthen anti-tumor immune response, and meanwhile, the product can interfere with a metabolic pathway of cancer cells and reverse the drug resistance of cancer cells, so that the anti-tumor effect is improved. Nutrition supply is blocked to inhibit tumor growth, the production process is green and controllable, and good clinical transformation and industrialization prospects are achieved.

The invention discloses a preparation method of low-moisture and low-water-activity xylooligosaccharide powder, and belongs to the field of functional sugar alcohol. The method comprises the following steps: taking a xylooligosaccharide solution with the refractive index of 35-50%, adding activated carbon for decoloration, filtering, adding deionized water to adjust the refractive index to 40-48%, and purifying through cation exchange resin, anion exchange resin, anion exchange resin and cation exchange resin in sequence; evaporating the purified solution until the refractive index is 50-55%; homogenizing the concentrated solution, spraying the homogenized concentrated solution into a spray drying tower through a spray head, and drying the homogenized concentrated solution in a heating evaporation area and a cooling collection area under the pressure of 100-150Pa in the tower; and drying, screening with a 60-mesh screen, feeding into a clean room, cooling, and packaging. The water content of the prepared xylooligosaccharide powder is 0.1%-1%, the water activity is 0.1% or below, the product fluidity is good, crushing treatment is not needed, the process is simple and efficient, and the xylooligosaccharide powder is suitable for industrial production.

The invention discloses a high-efficiency separation method of breast milk oligosaccharide and application of the breast milk oligosaccharide in specific recognition of influenza A virus hemagglutinin protein.The method comprises the steps that a DE-52 weak anion exchange column, a graphite carbon column and Bio-GelP10 column chromatography are combined for use, breast milk oligosaccharide components with low abundance and high molecular weight are efficiently enriched, and the specific recognition of influenza A virus hemagglutinin protein is achieved; after derivatization labeling of a bifunctional reagent 2-amino-N-(2-aminoethyl) benzamide (AEAB), 56 breast milk oligosaccharide monomers with the purity higher than 90% are separated from the isomer level through one-dimensional amide normal phase chromatography and two-dimensional C18 reverse phase circulation chromatography, and a monomer substance library is constructed. AEAB is used as a connecting arm to construct a breast milk oligosaccharide chip, an active monomer DSLNT II which is specifically combined with four subtype influenza A virus hemagglutinin proteins is screened out in a high-throughput manner, and fine structure analysis is completed in combination with electrospray tandem mass spectrometry. According to the method, the large-scale preparation of the low-abundance breast milk oligosaccharide monomer is realized, and a key material basis and theoretical guidance are provided for researching an antiviral mechanism and developing related functional foods and medicines.

The present invention relates to the use of a cosmetic active ingredient comprising an extract of the biomass of at least one yeast of the species Aureobasidium pullulans, characterized in that the extract contains at least 25% by weight of sugars based on the dry matter of the extract, as a cosmetic active ingredient in a use or composition comprising the same.

The invention relates to a method for the separation of two hydrophilic neutral oligosaccharides from each other with a chromatography on a bromine functionalized polystyrene cross-linked with divinylbenzene (BPS-DVB) stationary medium.

The invention discloses a marine polysaccharide degradation method and marine oligosaccharide, and relates to the technical field of marine polysaccharide degradation. The method comprises the following steps: S1, adding H2O2 into a 0.5%-2.5% (w / v) marine polysaccharide solution until the concentration of H2O2 in the solution reaches 1%-5% (w / v), and adjusting the pH value of the solution to 3-7 to obtain a first mixed solution; s2, performing microwave-ultrasonic treatment on the first mixed solution, and terminating the reaction after the microwave-ultrasonic treatment is completed to obtain a second mixed solution; s3, separating and purifying the second mixed solution, and freeze-drying to obtain the marine oligosaccharide. The method is easy and convenient to operate, green and environmentally friendly, the low-molecular-weight marine oligosaccharide can be efficiently prepared, and the prepared marine oligosaccharide is small in molecular weight and low in apparent viscosity.

The present invention relates to molecules having a soluble tag and methods for increasing the solubility of molecules. Additionally, the present invention relates to antibody-drug conjugates having a soluble tag, methods and compounds for preparing such antibody-drug conjugates, methods for increasing the solubility of antibody-drug conjugates, antibody-drug conjugates prepared by such methods, and the use of such antibody-drug conjugates in medicine.

This invention discloses a method for preparing a mixture of mussel oligosaccharides and the application of the product in inflammatory bowel disease. This invention uses mussel polysaccharide MP-1 as a raw material, processed by α-amylase or Bacillus subtilis (… Bacillus subtilis BNCC185269) obtained mussel oligosaccharide mixtures MOS-A and MOS-B composed of different oligosaccharides. Animal experiments have shown that the two oligosaccharide mixtures prepared by this invention can effectively alleviate intestinal inflammation. Therefore, the mussel oligosaccharide mixtures prepared by this invention are natural and safe drugs or foods for improving inflammatory bowel disease. They can effectively improve inflammatory bowel disease, are safe, non-toxic, and have no side effects. The raw materials are abundant, the steps are simple, the cost is low, and the effects are significant.

The present disclosure describes compositions and methods including liposome or lipid nanoparticles (LNPs) including carbohydrate-based ligands that target the liver, lungs, and / or kidneys. More particularly, the present disclosure relates to compositions and methods including liposome or lipid nanoparticles (LNPs) including carbohydrate-based ligands, such as di-lactobionic acid (LBA) ligand, and a suitable cargo such as nucleic acids and small molecule to target the liver, lungs, and / or kidneys for treatment and / or diagnosis of diseases and conditions.

The present invention relates to milk products comprising a high amount of in situ produced galactooligosaccharides (GOS) and methods of producing same. Milk substrate having a lactose content of at least 20wt% lactose is treated with an enzyme having transgalactosylating activity. The transgalactosylating activity of said enzyme may have been increased via glycation of lysine and / or arginine residues by incubation of said enzyme with high concentrations of glucose at elevated temperatures.

Described herein are polysaccharide polymers comprising a saccharide moiety modified with a hydroxyl-modifying agent (e.g., a saccharide monomer of Formula (I)), as well as related compositions, hydrogels, implantable elements, and methods of use thereof.

The invention relates to the field of chitosan oligosaccharide preparation, and mainly relates to a method for preparing chitosan oligosaccharide through uniform degradation of chitin in a high-solid-liquid-ratio system and the chitosan oligosaccharide. The invention discloses a method for preparing chitosan oligosaccharide by uniformly degrading chitin in a high-solid-liquid-ratio system. The method comprises the following steps: preparing an acid reaction solution; the acid reaction liquid comprises a medium strong acid or a compound liquid of the medium strong acid and a weak acid or a compound liquid of the strong acid and the weak acid; chitin and an organic reagent are stirred and mixed, an acid reaction solution is added, and the mass ratio of chitin to the acid reaction solution is 1: (2-5); after the chitin particles are deposited, pouring out the organic reagent to obtain a mixture; reacting the mixture for 6-12 hours under the condition of 20-80 DEG C; after the reaction is finished, carrying out solid-liquid separation to obtain a chitosan oligosaccharide mixed solution; and treating the chitosan oligosaccharide mixed solution to obtain the chitosan oligosaccharide. According to the present invention, the accurate and controllable preparation of the chitosan oligosaccharide in the aspects of molecular weight size, molecular weight distribution and product yield can be achieved, and the stability of the product quality and the operability of the process can be significantly improved.

The present invention relates to the field of modified proteins, immunogenic compositions and vaccines comprising the modified proteins, their manufacture and the use of such compositions in medicine. More particularly, it relates to a modified Sap2 (Secreted Aspartyl Proteinase 2 of Candida albicans') protein. The modified Sap2 protein can be used as a carrier protein for other antigens, particularly saccharide antigens or other antigens lacking T cell epitopes.

This invention relates to the field of agricultural and forestry waste treatment and high-value utilization technology, specifically to a method for co-producing furfural, oligodextrose, and lignin from agricultural and forestry waste. The method includes the following steps: mixing agricultural and forestry waste with a p-toluenesulfonic acid solution and performing a first heat treatment; after the reaction, solid-liquid separation is performed to obtain a first filtrate and a first residue; adding aluminum chloride and methyl isobutyl ketone to the first filtrate and performing a second heat treatment; after the reaction, separating the organic and aqueous phases, collecting the organic phase, and distilling to obtain furfural; washing the first residue to neutrality, adding it to a lithium chloride solution, and adding HZSM-5 molecular sieves for a third heat treatment; after the reaction, solid-liquid separation is performed to obtain a second filtrate and a second residue; concentrating the second filtrate and adding methanol, collecting the precipitate as oligodextrose; adding acidic dioxane / water to the second residue, dissolving it completely, performing solid-liquid separation, collecting the third filtrate, and removing the dioxane solvent to obtain lignin.

This invention relates to the fields of functional foods and bioengineering technology, specifically to a soybean oligosaccharide extract, its processing method, and its application in weight management. It aims to address the problems of low extract purity, high energy consumption, and significant environmental pollution in existing technologies. This invention employs a three-stage tandem extraction process for soybean raw materials, effectively removing fat-soluble impurities and easily fermentable small-molecule sugars; it utilizes ceramic membrane ultrafiltration to remove large-molecule impurities; and then concentrates the extract at low temperature using rotating cone molecular distillation. Furthermore, this invention employs yeast-lactic acid bacteria dual fermentation to directionally transform byproducts, achieving comprehensive resource utilization. The soybean oligosaccharide extract obtained by this invention has stachyose and raffinose as its main components, with significantly improved purity. The extract of this invention can effectively reduce calorie intake and improve the bioavailability of polyphenols and other active substances, thereby helping to control weight gain.