423results about "Oligosaccharides" patented technology

The invention discloses oligochitosan oligosaccharide, oligochitosan oligosaccharide composite nanoparticles as well as a preparation method and application thereof, and belongs to the technical field of nano materials and food engineering. According to the method disclosed by the invention, the chitosan oligosaccharide with the polymerization degree of 2-6 is prepared by adopting an ultrasonic-microwave synergistic compound enzyme enzymolysis method, so that the yield and the quality of the chitosan oligosaccharide are improved. The chitosan oligosaccharide and zein are assembled to form the chitosan oligosaccharide composite nanoparticles, the chitosan oligosaccharide composite nanoparticles have the advantages of being small in particle size and high in stability, the antioxidant function of the chitosan oligosaccharide is remarkably improved, the preparation method is simple, convenient, economical and practical, and the chitosan oligosaccharide composite nanoparticles can be used as food, health care products and medicine carrier materials and have good market application prospects.

The application provides a Huangdache oligosaccharide with GLP-1 agonist activity and application thereof, and belongs to the technical field of medicines. 3 The oligosaccharide component ELYP-3 has a molecular weight of 3.2*10 3 Da, and the molar ratio of monosaccharides is rhamnose:galacturonic acid:glucose:galactose:arabinose = 1:32.42:45.37:9.11:6.54. The Huangdache oligosaccharide fragment obtained by glycosidase degradation has significant hypoglycemic and weight loss activities, the preparation process is simple, specific and green, and provides scientific basis and theoretical support for the development of natural GLP-1 agonists. Meanwhile, the functional oligosaccharide provides a wide application prospect for the development of medicines with blood glucose regulation.

The invention relates to the technical field of biology, and particularly discloses a method for removing lactulose-based isomers in an oligosaccharide separation and purification process. The method comprises the following steps: taking oligosaccharide fermentation liquor for producing oligosaccharide through fermentation by using lactose as a substrate, sequentially carrying out thallus removal, decoloration, electrodialysis desalination, ion exchange resin desalination, concentration, sequential simulated moving bed chromatographic purification, ultrafiltration, re-concentration and spray drying, and detecting the oligosaccharide and the lactulose-based isomer in the oligosaccharide. According to the method disclosed by the invention, the content of lactulose-based isomers in the separation and purification process is greatly reduced, so that the product purity is effectively improved; the method can be applied to the synthesis process of other milk oligosaccharides such as fucose lactose (FL), lactose-N-tetraose (LNT), lactose-N-neotetraose (LNnT), lactose-N-fucopentasaccharide (LNPFI) and the like or other carbohydrates containing a lactosyl group, has a very high application value, and accords with laws and regulations.

Described are methods of treating a neurodegenerative disease, such as an age- related neurodegenerative disease in a patient in need thereof, comprising administering a composition comprising a therapeutically effective amount of at least one human milk oligosaccharide (HMO). Also described are methods of treating Parkinson's Disease (PD) in a patient in need thereof comprising administering to said patient an effective amount of an HMO.

The invention relates to a preparation method of ginkgo oligosaccharide and a ginkgo oligosaccharide sugar chain plant vaccine, and relates to the field of plant vaccines. The preparation method of the ginkgo oligosaccharide comprises the step that the ginkgo oligosaccharide is extracted from ginkgo testa through an enhanced extraction method, the enhanced extraction method is an extraction method combining an ultrasonic extraction method and a water extraction and alcohol precipitation method, the purity of the oligosaccharide is improved, the structure of the oligosaccharide is not changed, and the cost is reduced. The ginkgo oligosaccharide is used for preparing the plant vaccine, and the vaccine takes the ginkgo oligosaccharide extracted from ginkgo testa as a main component, and has the function of inducing plants to generate stress resistance such as disease resistance, drought resistance, cold resistance and hot air resistance.

The present disclosure describes compositions and methods including liposome or lipid nanoparticles (LNPs) including carbohydrate-based ligands that target the liver, lungs, and / or kidneys. More particularly, the present disclosure relates to compositions and methods including liposome or lipid nanoparticles (LNPs) including carbohydrate-based ligands, such as di-lactobionic acid (LBA) ligand, and a suitable cargo such as nucleic acids and small molecule to target the liver, lungs, and / or kidneys for treatment and / or diagnosis of diseases and conditions.

To provide a binding syrup composition for formulating food products containing allulose and low sugar syrups.SOLUTION: The binding syrup composition overcomes limitations of allulose-containing products including product stickiness and failure to maintain shape and texture of the products. More specifically, the binding syrup compositions described herein contain low sugar syrups with low mono- and di-saccharide content in combination with allulose to impart binding and textural attributes to the allulose-containing food products that are comparable to full sugar products. Food products containing the allulose binding syrup compositions described herein and methods of producing the same are also provided. Beneficially, food products having reduced caloric and sugar content are provided when employing the allulose-containing binding syrup compositions described herein.SELECTED DRAWING: Figure 1

The present invention provides methods for glycosylation and compound preparation. The compounds include galactosylated, sialylated, fucosylated, and N-acetylglucosaminated compounds from simple oligosaccharides and sugars of animal, plant, or microbial origin. In certain embodiments, the invention provides for trinucleotide-free enzymatic production of oligosaccharides starting from simple sugars, including plant-based sugars. The present invention also provides methods of enzymatic production of fucosylated oligosaccharides and fucosylated antibody-glycan conjugates from ordinary enzymes. The production may be a cell-free, one-pot synthesis using an enzyme and, in some embodiments, an immobilized enzyme. The synthesis is a highly customizable and efficient cell-free manufacturing process. In some embodiments, lactose derivatives and human milk oligosaccharides (HMOs) are produced.

The invention relates to the field of xylooligosaccharide production, and discloses a xylooligosaccharide production method, which comprises: raw material pretreatment: removing impurities from a raw material rich in lignocellulose, and crushing; acid liquid is sprayed into the fine powder, mixing and stirring are conducted at the same time, and heating is conducted for a sugar soaking reaction; gradient sugar washing: adding deionized water into the residue sugar mixture to carry out multiple rounds of gradient sugar washing, and separating to obtain a high-concentration sugar solution; electrodialysis acid recovery: carrying out deacidification treatment on the high-concentration sugar solution by using electrodialysis, and recovering the acid solution for reutilization; and continuous separation and preparation of xylooligosaccharide: adjusting the pH value of the sugar solution to 4.0-6.5, separating the xylooligosaccharide by adopting a nanofiltration membrane, and adding xylanase into a nanofiltration membrane concentration tank at the same time, so that the intercepted xylan is continuously degraded into the xylooligosaccharide. According to the method, xylooligosaccharide separation and xylooligosaccharide preparation are combined together, so that xylooligosaccharide obtained by xylanase hydrolysis can penetrate through a nanofiltration membrane in time, xylooligosaccharide is prevented from being continuously hydrolyzed into xylose, and generation of by-products is reduced.

The preparation method comprises the following steps: (1) dissolving agar in a citric acid monohydrate solution, and carrying out acidolysis to obtain an acidolysis solution; centrifuging, filtering, adding beta-agarase AgWH50B, and carrying out enzymolysis for 20-28 hours at the temperature of 30-35 DEG C; boiling, centrifuging and filtering to obtain supernate, namely crude oligosaccharide liquid containing agarotriose; (2) dissolving agar in a phosphoric acid solution, and degrading for 55-65 minutes at the temperature of 97-103 DEG C; centrifuging and filtering to obtain supernate, namely crude oligosaccharide liquid containing agarobiose; (3) dissolving agar in deionized water, adding alpha-agarase A33, and carrying out enzymolysis at 38-42 DEG C for 20-28 hours; and boiling, centrifuging and filtering to obtain supernate, namely crude oligosaccharide liquid containing agarotetraose. The preparation method of the agaro-oligosaccharide is used for preparing agarotriose, agarobiose and agarotetraose, and the product purity is high. The method can provide guidance for subsequent large-scale industrial production of agaro-oligosaccharide.

The invention discloses a method for preparing high-purity brown algae oligosaccharide, and belongs to the field of marine chemical technology. The method for preparing brown algae oligosaccharide of the invention comprises the following steps: after sodium alginate is divided into three times for enzymatic hydrolysis, the enzymatic hydrolysis liquid is ultrafiltered to obtain a brown algae oligosaccharide clear liquid; the brown algae oligosaccharide clear liquid is spray-dried to obtain the brown algae oligosaccharide. In the invention, the sodium alginate has a high degree of degradation, the obtained brown algae oligosaccharide has a high purity, and the production process is safe and efficient.

The application discloses a high-precision intelligent control and adjustment method for stachydrine fermentation and purification, and relates to the field of biological fermentation process control. The method comprises the following steps: obtaining a stachydrine mixed solution in a first sugar content interval; inoculating Eurotium cristatum at a first preset temperature after sterilization to start fermentation and obtain an initial fermentation liquor; monitoring the content ratio change of stachydrine and non-target sugars in real time through HPLC to obtain sugar content monitoring data; introducing a membrane separation device for selective dialysis to obtain membrane permeation rate monitoring data; performing dynamic evaluation on stachydrine enrichment according to the sugar content monitoring data and the membrane permeation rate monitoring data, generating a dynamic adjustment strategy for fermentation conditions based on the evaluation results; and performing fermentation process adjustment control. The method solves the technical problem that the existing stachydrine fermentation and purification cannot realize real-time and accurate monitoring and dynamic adjustment of fermentation conditions, resulting in low fermentation efficiency and low purity, and achieves the technical effect of improving the fermentation efficiency and purity of stachydrine through intelligent control of the fermentation process.

The invention relates to the technical field of breast milk oligosaccharide production, and particularly to an Escherichia coli K-12 MG1655 BLBYZT6 and an application thereof. The Escherichia coli K-12 MG1655 BLBYZT6 is preserved in the China General Microbiological Culture Collection Center, with the collection number of CGMCC No. 28317, the collection date of Aug. 31, 2023, and the collection institution address of No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The Escherichia coli K-12 MG1655 BLBYZT6 of the invention is applied in fermentation production of 2′-fucosyllactose and difucosyllactose. The invention improves a yield of the 2′-fucosyllactose, reduces a dry matter proportion of a non-target product, realizes simple separation and purification, is beneficial for industrial production, and can co-produce the difucosyllactose with a purity greater than 90% at the same time.

Aspects of the disclosure relate to oligosaccharide compositions and methods of making the same. Also provided are methods of using oligosaccharide compositions as microbiome metabolic therapies for reducing ammonia levels and for the treatment of hyperammonemia-related diseases (e.g., urea cycle disorders and hepatic encephalopathy).

The invention relates to a method for simultaneously extracting dietary fiber and oligosaccharide from beet pulp, which comprises the following steps: mixing pretreated beet pulp with water, extracting, filtering to obtain a first filter residue and a first supernatant, mixing the first filter residue with alkali, and centrifuging to obtain a second filter residue and a second supernatant; mixing the second filter residue with an acid, and centrifuging to obtain a third filter residue and a third supernatant; mixing the third filter residue with an enzyme for enzymolysis, and centrifuging to obtain a fourth filter residue and a fourth supernate; mixing all the supernatants, performing ultrafiltration concentration to obtain a concentrated solution with the molecular weight of 500-10kDa, and performing freeze drying to obtain oligosaccharide; the concentrated solution with the molecular weight larger than 10 kDa is subjected to ethanol precipitation, and soluble dietary fibers are obtained; and mixing the fourth filter residue with a hydrogen peroxide solution, boiling, and filtering to obtain a precipitate which is the insoluble dietary fiber. According to the method, three substances are simultaneously extracted in one technological process, so that resources are comprehensively utilized, and the extraction efficiency is maximized.

The present invention aims to provide a saccharide composition suitable for a cyclic-tetrasaccharide-containing starch syrup which has low viscosity, low water activity, low coloration property, and low calorie content, and which is unlikely to cause precipitation of crystals of saccharides during storage, and to provide a use of the composition and a production method for the composition. The object is achieved by providing a cyclic-tetrasaccharide-containing saccharide composition having the following characteristics (1) to (3): (1) the saccharide composition includes a branched cyclic tetrasaccharide in addition to the cyclic tetrasaccharide, wherein the content of the cyclic tetrasaccharide with respect to the total solid content of the saccharide composition obtained by allowing glucoamylase and α-glucosidase to act on the above saccharide composition is 38% by mass or higher, on a dry solid basis; (2) the ratio of α-1,4-linked glucose in the total glucose residues constituting the saccharide composition in methylation analysis is over 9% and 15% or lower; and (3) the ratio of α-1,4,6-linked glucose in the total glucose residues constituting the saccharide composition in methylation analysis is less than 6%; and providing a use of the composition and a production method for the composition.

The invention discloses a method for extracting and preparing an oligosaccharide extract from codonopsis pilosula and application of the oligosaccharide extract in prevention and treatment of neurodegenerative disease Alzheimer's disease, and belongs to the technical field of traditional Chinese medicine pharmacy. According to the present invention, the oligosaccharide extract is enriched and prepared from Codonopsis pilosala (Franch.) Nanf., wherein the Codonopsis pilosala (Franch.) Nanf. Is a plant of the family of Platycodon grandiflorum, and the activity test of the oligosaccharide extract on the improvement of cognitive dysfunction in the animal body is performed; results show that in a mouse cognitive function impairment model caused by sleep deprivation, the high-dose codonopsis pilosula oligosaccharide has a prominent effect on improving the learning and memory ability of a Morris water maze and a neuroprotective effect, the efficacy of the codonopsis pilosula oligosaccharide is equivalent to that of a positive control drug manntranet, and no obvious toxic or side effect is found. The codonopsis pilosula oligosaccharide is expected to be further developed into functional food, health care products and / or medicines with the effect of preventing and / or improving cognitive impairment.

The method comprises the following steps: S1, adding a formic acid-sodium formate solution into raw material powder, carrying out high-temperature oil bath, cooling to room temperature, carrying out suction filtration to obtain a supernatant A containing xylooligosaccharide, repeatedly cleaning and carrying out suction filtration until the extracted liquid is neutral, and drying at low temperature to obtain the xylooligosaccharide powder; pre-treated lignin raw materials are obtained; s2, putting the supernate into a dialysis bag, and magnetically stirring to obtain primary dialysis liquid B; s3, putting the preliminary dialysis liquid B into a dialysis bag, and magnetically stirring to obtain dialysis liquid C; s4, concentrating the dialysis liquid C to obtain concentrated liquid D; and S5, pre-freezing the concentrated liquid D, and freeze-drying the sample to obtain xylooligosaccharide powder E. According to the method, formic acid-sodium formate is used as a catalytic medium, different acid concentrations and molar ratios are adopted, high-temperature oil bath and dialysis freeze-drying are utilized for two-step extraction, and the yield of xylooligosaccharide after catalysis is obviously increased.

The present invention relates to a pentose intermediate of fondaparinux sodium. It uses an automated system with automatic sample loading and automatic sampling and monitoring to automate the production of the three components (D+EF+GH), obtaining the fully protected pentose intermediate of fondaparinux sodium (Formula I). ​​This automated synthesis of the pentose intermediate of fondaparinux sodium saves labor, improves efficiency and productivity, and provides high safety and reproducibility. Direct online monitoring facilitates real-time optimization and monitoring of the reaction. Automatic temperature control better meets the needs for temperature rise and fall. A "pre-activation" one-pot mode is adopted, reducing the number of isolation steps and simplifying the operation. The commonly used ester protecting group is used, resulting in high stereoselectivity and yield, and a commonly used deprotection method can be used. [Formula 1] JPEG2023553939000024.jpg53170

Disclosed is a wood-derived carbohydrate composition, wherein the carbohydrate composition comprises at least 40 weight-% of carbohydrates based on the total dry matter content of the carbohydrate composition, at least 30 weight-% of the carbohydrates are xylose, and at least 50 weight-% of the xylose is oligomeric xylose. Further is disclosed the use of the wood-derived carbohydrate composition.

The application discloses a method for extracting soybean saponins and soybean oligosaccharides, and belongs to the technical field of natural product extraction. The method comprises the following steps: puffing treatment of soybean meal, wall breaking treatment in a high-voltage electric field; acid precipitation, collection of supernatant; after ultrasonic oscillation of the supernatant, a composite enzyme is added for enzymolysis, and a material liquid after enzymolysis is obtained; after filtration of the material liquid, the material liquid is loaded onto a macroporous resin column for adsorption, and after completion of the adsorption, effluent is collected; the effluent is purified and concentrated to obtain soybean oligosaccharides; an ethanol solution is used to elute the resin column, and eluate is collected; the eluate is recovered by ethanol and dried to obtain soybean saponins; the composite enzyme comprises amylase and beta-glucosidase. The method can significantly improve the extraction rate and purity of soybean saponins and soybean oligosaccharides, the whole process uses water as the main solvent, the consumption of organic solvents and the pollution to the environment are reduced, and the unit process water consumption, unit energy consumption and unit cost are reduced.

The invention discloses a Enteromorpha oligosaccharide and its preparation method and application. The Enteromorpha oligosaccharide is prepared by degradation of Enteromorpha polysaccharide, has a degree of polymerization of 2-10, contains rhamnose, glucose, glucuronic acid and xylose, and has a simple preparation method. The present invention verifies for the first time that Enteromorpha oligosaccharide can significantly reduce the fasting blood sugar level of Type 2 diabetic mice, reduce the insulin content and glycosylated serum protein level in serum, improve the blood lipid metabolism ability of Type 2 diabetic mice, improve the glycogen synthesis ability in the liver of mice, improve the liver function abnormality of Type 2 diabetic mice, and Enteromorpha oligosaccharide has high safety, and has good prospects in developing food or medicine with the function of controlling Type 2 diabetes.

This invention discloses an acid-catalyzed controlled degradation and recycling method for waste polyester and polyester-cotton blended fabrics. Using waste polyester or waste polyester-cotton blended fabrics as raw materials, under the action of an acidic catalyst, in a mixed solvent composed of a polar aprotic solvent and water, the method involves heating and degradation, causing the polyester in the waste polyester or polyester-cotton blended fabrics to depolymerize or co-depolymerize, generating organic raw materials. This invention achieves complete depolymerization and recycling of polyester with a relatively low acid dosage, effectively avoiding the corrosion problems of equipment and excessive degradation of cotton fibers caused by existing technologies. It is also more conducive to the generation and recovery of glucose and 5-hydroxymethylfurfural. Furthermore, the separation steps are simple, greatly reducing separation costs, and high-yield recovery of terephthalic acid, glucose, and 5-hydroxymethylfurfural is achieved, realizing high-value recycling and utilization of various components in textiles, reducing waste, and improving resource utilization.

The present disclosure discloses the identification and introduction of a specific heterologous gene encoding an alpha-1,2-fucosyltransferase (denoted smob) into LNT producing strains for the specific production of LNFP-I. The smob gene is derived from the microorganism Sulfuriflexus mobilis (https: / / www.dsmz.de / collection / catalogue / details / culture / DSM-102939), a sulfur-oxidizing bacterium isolated from a saline lake sediment.

This invention relates to a process for the production and purification of fructooligosaccharides (FOS) and the product obtained by such process having low or no residue of glucose, or fructose, or sucrose, with the FOS having a different ratio of oligomers (GF2, GF3, GF4, GF5 and GF6) and their isomers, to be used in animal and human nutrition due to their superior properties of modulating the intestinal microbiota and direct and indirect immunomodulation. The process for producing and purifying fructooligosaccharides according to the invention employs at least two microbes or yeasts, simultaneously or separately, with one of the microbes or yeasts being unable to produce extracellular invertase in a medium containing sucrose, in order to convert sucrose into fructooligosaccharides (FOS), with total consumption of glucose from the medium and evaporation of alcohol or ethanol during drying of the final product.

A binding syrup composition for formulating food products containing allulose and low sugar syrups is provided. The binding syrup composition overcomes limitations of allulose-containing products including product stickiness and failure to maintain shape and texture of the products. More particularly, the binding syrup compositions described herein contain low sugar syrups with low mono- and di-saccharide content in combination with allulose to provide binding and textural attributes to the allulose-containing food products that are comparable to full sugar products. Food products containing the allulose binding syrup compositions described herein and methods of making the same are also provided. Beneficially, food products having reduced caloric and sugar content are provided when employing the allulose-containing binding syrup compositions described herein.

The present disclosure relates to the production of complex fucosylated Human Milk Oligosaccharides (HMOs) and in particular to the production of complex fucosylated HMOs with five or more monosaccharide units, such as LNFP-III, LNFP-VI and LNDFH-III as well as mixtures thereof. The present disclosure also relates to genetically engineered cells and α-1,3-fucosyltransferases suitable for use in said production, as well as to methods for producing said fucosylated HMOs.

The present invention provides a new antibacterial compound named nidaramycin, which is derived from a novel biosynthetic gene cluster (BGC), and its use. In addition, this specification provides novel genes and nucleic acid molecules encoding the biosynthetic machinery for the production of nidaramycin, as well as constructs, vectors, and host cells for expressing the BGC, and methods for producing the compound.