The invention discloses a carbohydrate composition containing yeast beta-glucan. The carbohydrate composition is formed by combining yeast beta-glucan with oligosaccharide in the mass ratio of the yeast beta-glucan to the oligosaccharide being (1 to 1000) to (1000 to 1), wherein the oligosaccharide is one or several kinds of fructo-oligosaccharide, isomalto-oligosaccharide, lactose-oligosaccharide, galactooligosaccharide, and xylooligosaccharide; and the carbohydrate composition also comprises one or several kinds of monosaccharide, disaccharide, polysaccharide, maltodextrin and glucose polymers. The invention further discloses an application of the carbohydrate composition for formula foods with special medicine use, wherein the percentage by mass of the carbohydrate composition is 1-90%. The yeast beta-glucan is non-toxic and free from allergic reaction, a special molecular structure can specially activate the immunocyte of organisms, the immune function of the organisms is strengthened, the carbohydrate composition containing the yeast beta-glucan can provide energy, improve immunity, improve the functions of the intestine and the stomach before the operation and can be made into the formula foods to be convenient for patients to use, and the recovery function of the patients after the operation is improved.