Analysis method for measuring carbohydrate composition of polysaccharide

An analytical method and technology for sugar components, which are applied in analytical materials, measuring devices, material separation, etc., can solve the problems of difficult quantitative analysis, poor separation effect, low sensitivity, etc., and achieve the effects of high content, easy operation and high sensitivity

CN104007201AInactive Publication Date: 2014-08-27SOUTH CHINA UNIV OF TECH

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Patent Information

Authority / Receiving Office: CN · China
Current Assignee / Owner: SOUTH CHINA UNIV OF TECH
Publication Date: 2014-08-27
Estimated Expiration: Not applicable · inactive patent

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Abstract

The invention belongs to the technical field of qualitative and quantitative analysis for polysaccharides, and particularly relates to an analysis method for measuring a carbohydrate composition of a polysaccharide. The analysis method for measuring the carbohydrate composition of the polysaccharide comprises the following steps: adequately dissolving the polysaccharide to be measured and an ionic liquid in an oil bath pan to obtain a polysaccharide dissolution liquid, cooling the polysaccharide dissolution liquid and then pouring in a regeneration solvent to obtain a precipitate, repeatedly washing, centrifuging to obtain a regenerated polysaccharide crude product, ventilating to completely volatilize the residual regeneration solvent, and dying to obtain the regenerated polysaccharide; putting the regenerated polysaccharide and an acid solvent in an ampere bottle, sealing, putting the ampere bottle in the oil bath pan and carrying out hydrolysis reaction, centrifuging after the hydrolysis reaction is finished, and carrying out detection pre-treatment on supernatant to obtain a polysaccharide detection liquid; and analysing the type and the content of reducing sugar of the carbohydrate composition in the polysaccharide detection liquid by adopting an ion chromatograph system and an ion chromatograph-pulse amperometry. According to the analysis method disclosed by the invention, the hydrolysis efficiency of the polysaccharide is improved, and the extremely high sensitivity is achieved.

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Description

technical field

[0001] The invention belongs to the technical field of qualitative and quantitative analysis of sugar components in polysaccharides, and in particular relates to an improved analysis method for determining the sugar components of polysaccharides. Background technique

[0002] Polysaccharides are one of the most abundant substances in nature and play a vital role in maintaining life activities. In the life process, polysaccharides not only serve as energy substances and structural substances, but also have various biological activities such as anti-tumor, anti-aging, anti-virus, immune regulation and hypoglycemic, and are widely used in health food, medicine and clinical practice.

[0003] Polysaccharide structure analysis is very important for polysaccharide research: to reveal the structure-activity relationship of polysaccharides and to develop new functional polysaccharides. However, in view of the complexity of polymer polysaccharides, there is no direct...

Claims

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