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Analysis method for measuring carbohydrate composition of polysaccharide

An analytical method and technology for sugar components, which are applied in analytical materials, measuring devices, material separation, etc., can solve the problems of difficult quantitative analysis, poor separation effect, low sensitivity, etc., and achieve the effects of high content, easy operation and high sensitivity

Inactive Publication Date: 2014-08-27
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The currently reported methods for the determination of monosaccharides mainly include gas chromatography, liquid chromatography, affinity chromatography, enzymology and capillary electrophoresis, etc. These analytical methods generally have low sensitivity and poor separation effect. The current general method is gas chromatography method, which solves the problem of partial separation of various isomers of monosaccharides by the above instruments, but this method requires complex derivatization treatment, and at the same time, the loss of monosaccharides is also caused during the derivatization process, which brings great inconvenience to quantitative analysis. difficulty
Ion chromatography-pulse amperometric sugar detection is a new method developed in recent years. This method can analyze almost all monosaccharides and most oligosaccharides and oligosaccharides without derivatization. It is easy to operate and does not require complicated sample pretreatment, let alone derivatization. , and has extremely high sensitivity, and can usually detect sugars at the pmoL level, but combined with acid hydrolysis to analyze the sugar components and content in polysaccharides with high crystallinity, there is no report

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  • Analysis method for measuring carbohydrate composition of polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A method for measuring polysaccharide sugar components, the specific steps are as follows:

[0038] 1. Ionic liquid pretreatment and polysaccharide regeneration: Weigh 0.2g of yeast β-glucan dried in a vacuum oven at 70°C with a vacuum degree of 0.098kPa for 2h at a constant temperature, mix it with 4g of regeneration solvent [BMIM]Cl in oil Fully dissolve in the bath for 2 hours to obtain yeast β-glucan solution; add yeast β-glucan solution to 5 times the volume of ionic liquid in absolute ethanol for regeneration, rinse repeatedly, and centrifugally settle to obtain regenerated yeast β-glucan - Crude glucan product, place the crude product of regenerated yeast β-glucan in a fume hood to fully volatilize the regeneration solvent, and then dry it for 5 hours in an environment with a temperature of 50°C and a vacuum of 0.1KPa to obtain the regenerated yeast β-glucan sugar;

[0039] 2. Hydrolysis with acid solvent: mix the regenerated yeast β-glucan obtained in step 1 wi...

Embodiment 2

[0045] A method for measuring polysaccharide sugar components, the specific steps are as follows:

[0046] 1. Ionic liquid pretreatment and polysaccharide regeneration: Weigh 0.2g of Cordyceps polysaccharide that was dried at a constant temperature in a vacuum oven with a vacuum degree of 0.098kPa at 50°C for 3h, mix it with 3g of regeneration solvent [Emim]Ac in an oil bath and fully Dissolve for 3 hours to obtain a solution of Cordyceps polysaccharide; add the solution of Cordyceps polysaccharide to deionized water with 10 times the volume of ionic liquid for regeneration, wash repeatedly, and centrifugally settle to obtain the crude product of regenerated Cordyceps polysaccharide, and place the crude product of regenerated Cordyceps polysaccharide in a fume hood Fully volatilize the regenerated solvent in the medium, and then dry for 4 hours in an environment with a temperature of 75°C and a vacuum of 0.08KPa to obtain the regenerated Cordyceps polysaccharide;

[0047] 2. A...

Embodiment 3

[0054] A method for measuring polysaccharide sugar components, the specific steps are as follows:

[0055] 1. Ionic liquid pretreatment and polysaccharide regeneration: Weigh 0.2g of condensed polysaccharide that was dried in a vacuum oven at 60°C with a vacuum degree of 0.098kPa for 4 hours, and mix it with 5g of regeneration solvent [Amim]Cl in an oil bath. Dissolve for 4 hours to obtain the coagulated polysaccharide solution; add the coagulated polysaccharide solution to anhydrous ethanol with 5 times the volume of the ionic liquid for regeneration, wash repeatedly, and centrifugally settle to obtain the crude product of the regenerated coagulated polysaccharide, and place the crude product of the regenerated coagulated polysaccharide in ventilation Fully volatilize the regenerated solvent in the cabinet, and then dry for 2 hours in an environment with a temperature of 80°C and a vacuum of 0.05KPa to obtain the regenerated coagulated polysaccharide;

[0056] 2. Acid solvent...

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Abstract

The invention belongs to the technical field of qualitative and quantitative analysis for polysaccharides, and particularly relates to an analysis method for measuring a carbohydrate composition of a polysaccharide. The analysis method for measuring the carbohydrate composition of the polysaccharide comprises the following steps: adequately dissolving the polysaccharide to be measured and an ionic liquid in an oil bath pan to obtain a polysaccharide dissolution liquid, cooling the polysaccharide dissolution liquid and then pouring in a regeneration solvent to obtain a precipitate, repeatedly washing, centrifuging to obtain a regenerated polysaccharide crude product, ventilating to completely volatilize the residual regeneration solvent, and dying to obtain the regenerated polysaccharide; putting the regenerated polysaccharide and an acid solvent in an ampere bottle, sealing, putting the ampere bottle in the oil bath pan and carrying out hydrolysis reaction, centrifuging after the hydrolysis reaction is finished, and carrying out detection pre-treatment on supernatant to obtain a polysaccharide detection liquid; and analysing the type and the content of reducing sugar of the carbohydrate composition in the polysaccharide detection liquid by adopting an ion chromatograph system and an ion chromatograph-pulse amperometry. According to the analysis method disclosed by the invention, the hydrolysis efficiency of the polysaccharide is improved, and the extremely high sensitivity is achieved.

Description

technical field [0001] The invention belongs to the technical field of qualitative and quantitative analysis of sugar components in polysaccharides, and in particular relates to an improved analysis method for determining the sugar components of polysaccharides. Background technique [0002] Polysaccharides are one of the most abundant substances in nature and play a vital role in maintaining life activities. In the life process, polysaccharides not only serve as energy substances and structural substances, but also have various biological activities such as anti-tumor, anti-aging, anti-virus, immune regulation and hypoglycemic, and are widely used in health food, medicine and clinical practice. [0003] Polysaccharide structure analysis is very important for polysaccharide research: to reveal the structure-activity relationship of polysaccharides and to develop new functional polysaccharides. However, in view of the complexity of polymer polysaccharides, there is no direct...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N27/48
Inventor 王兆梅廖伟李满凤
Owner SOUTH CHINA UNIV OF TECH
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