2results about How to "Easily hydrolyzed" patented technology

The application discloses a method for preparing high-yield galacturonic acid by combining chemical and biological methods, which comprises the following steps: firstly, mixing gelidium and a maleic acid solution, and performing microwave heating and acidolysis to obtain D-galactose; and then taking engineered Pseudomonas putida as a biological catalyst to efficiently oxidize the D-galactose into galacturonic acid. In the application, the D-galactose obtained in the microwave heating and acidolysis step has high concentration and high yield, and the D-galactose can be completely oxidized into galacturonic acid in the subsequent biological catalysis step. The method does not need strong acid or special catalyst, is green and environment-friendly, has low raw material cost, simple process, high galacturonic acid yield, and realizes high-value development and utilization of the biomass gelidium.

This invention provides a method for preparing oat polypeptides and their applications and compositions, relating to the field of cosmetics technology. The preparation method of this invention includes the following steps: (1) oat swells, is dried after steam explosion treatment, and then pectinase, cellulase, and amylase are added for enzymatic hydrolysis of carbohydrates to obtain enzymatically hydrolyzed oat residue; (2) a complex protease is added to the enzymatically hydrolyzed oat residue, and a first enzymatic hydrolysis is performed under the condition of 3-8% volume concentration of ethanol to obtain enzymatic hydrolysate A; the complex protease is papain, bromelain, and flavor protease in a mass ratio of 3-6:3-6:2; (3) trypsin is added to enzymatic hydrolysate A for a second enzymatic hydrolysis to obtain enzymatic hydrolysate B; (4) enzymatic hydrolysate B is ultrafiltered to obtain oat polypeptides. This application obtains oat polypeptides with small molecular weight and high content, which, when used in the preparation of cosmetics, have significant moisturizing, anti-wrinkle, and skin roughness-reducing effects.