Preparing method of gynostemma pentaphyllum extraction product rich in gypenosides

A technology of Gynostemma pentaphyllum extract and Gypenoside, applied in the field of preparation of Gynostemma pentaphyllum extract, which can solve the problems of low yield and consumption of medicinal resources

Inactive Publication Date: 2014-07-09
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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If traditional methods are used for separation, not only a large amou

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  • Preparing method of gynostemma pentaphyllum extraction product rich in gypenosides
  • Preparing method of gynostemma pentaphyllum extraction product rich in gypenosides

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Embodiment 1

[0023] The preparation of embodiment 1 Gynostemma pentaphyllum extract

[0024] 1. Preparation of Gynostemma pentaphyllum ethanol extract before fermentation

[0025] Get Gynostemma pentaphyllum and use 8 times the amount (v / w, mL / g) of 80% ethanol to reflux extract 3 times, each time for 2h, concentrate to obtain Gynostemma pentaphyllum ethanol extract; in plantaractobacillus medium, directly freeze-dry to obtain fermented Ex Gynostemma ethanol extract.

[0026] 2. Preparation of Gynostemma pentaphyllum fermented re-extract

[0027] Get Gynostemma pentaphyllum and use 8 times the amount (v / w, mL / g) of 80% ethanol to reflux extract 3 times, each time for 2h, concentrate, the concentrated solution was inoculated with Lactobacillus plantarum, Arthrobacter, Bacillus subtilis, yeast and In the culture medium of Lactobacillus bulgaricus, ferment at 29°C for 72 hours, extract with equal volume of n-butanol three times, combine the n-butanol extracts, concentrate under reduced pres...

Embodiment 2

[0036] Use the inoculation loop to take 2 loops of bacteria or yeast and put them into 150mL of sterilized medium and cultivate them for 24 hours as the experimental seed solution. The culture temperatures are Lactobacillus delbrueckii subsp. bulgaricus at 43°C and Lactobacillus plantarum at 37°C , Yeast, Bacillus subtilis subsp.

[0037] After adding the above-mentioned bacterial seed solution to 6 Erlenmeyer flasks each containing 150 mL of medium according to the inoculum amount of 5%, 500 μL of the fermentation substrate was added to each of them, and cultured for 24 hours at the same temperature and rotation speed as the seed solution.

[0038] After the fermentation is complete, add 50 μL of 1 mg / mL ginsenoside Rd standard substance to each bottle as an internal standard, extract twice with an equal volume of water-saturated n-butanol, combine the organic phases, concentrate under reduced pressure, and dissolve them to 0.22 μm with 3 mL of methanol Filter the membrane, a...

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Abstract

The invention provides a preparing method of a gynostemma pentaphyllum extraction product rich in gypenosides. The method includes fermenting a gynostemma pentaphyllum ethanol extraction product in a microbe culture medium at 27-40 DEG C for 1-7 days, extracting the fermented total gynostemma pentaphyllum extraction product with n-butanol with the same volume for 1-6 times, concentrating under vacuum, and drying to obtain a gynostemma pentaphyllum post-fermentation extraction product. The microbe is lactobacillus plantarum or saccharomycetes.

Description

technical field [0001] The present invention relates to the extraction of plant effective components, in particular, the present invention relates to a preparation method of Gynostemma pentaphyllum extract rich in Damulin A, Damulin B, gypenoside L and gypenoside LI. Background technique [0002] Gynostemma is the whole plant of Gynostemma pentaphyllum (Thunb.) Makino of Cucurbitaceae (Cucurbitaceae), Gynostemma pentaphyllum (Thunb.), which has the functions of clearing heat and detoxification, relieving cough and expectorant. Because Gynostemma pentaphyllum contains dammarane saponins similar to ginseng, it is also dubbed "Southern ginseng" and "Second ginseng". [0003] Saponins have many different biological activities. For example, ginsenoside Rb1 has sugar groups at C3 and C20, which can protect nerves and improve memory. Ginsenosides Rg3 and Rh2, which only have sugar groups at the C3 position, have strong antitumor activity. Studies have found that when the number o...

Claims

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Application Information

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IPC IPC(8): A61K36/424
Inventor 朴香淑李平曾志凯赵泮峰许啸王红亮李青云刘君地张赛田启宇
Owner CHINA AGRI UNIV
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